微生物代谢组学-肠道微生物代谢组学研究
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• Cumbersome detection process
• In-house database establishment essential
2.3 Metabolomics Products of Biotree
Untargeted Metabolomics
• Lipidomics • Medical Metabolomics • Pharmaco-Metabolomics • Plant Metabolomics • Microbial Metabolomics • Subcellular Metabolomics • Amino-Metabolomics
Metabolomics
The comprehensive and quantitative analysis of all metabolites of the biological system under study".(Oliver Fiehn, 2001)
1.2 A correlation between the main omics strategies used in systems biology studies
1.1 A Brief History of Metabolomics and ItsRelevance in Systems Biology
Metabolome
The change in the relative concentrations of metabolites as the result of deletion or overexpression of a gene.(Oliver et al., 1998)
1.6 Metabolic signatures of chronic diseases unveiled by metabolomics
Part 2: Workflow for studies in metabolomics
2.1 Workflow for studies in metabolomics
1.2 Current Major Research Labs in Metabolomics
Oliver Fiehn | University of
Gary Siuzdak | the Scripps
California, Davis, US
Research Institute, La Jolla,
• Targeted metabolites measurement
• Clinical biomarkers validation
Widely-targeted Metabolomics
• Metabolites Measurement • Potential biomarkers disovery • Focused on metabolites in the
Xijun Wang |
Heilongjiang University Weijia | Shanghai
Huiru Tang | Fudan Of Chinese Medicine, University, Shanghai, Haerbin, China
Guowang Xu | Dalian
China
An untargeted metabolomics approach is based
primarily on the qualitative or semiquantitative analysis of the largest possible number of metabolites from a diversity of chemical and biological classes contained in a biological specimen.
2.2 Metabolomics Research Caቤተ መጻሕፍቲ ባይዱegories
Technology Objective
Untargeted Metabolomics
• Metabolites detection • Potential biomarkers
disovery
Targeted Metabolomics
1.5 Novel biomarkers for pre-diabetes identified by metabolomics
Rui Wang-Sattler et al., Novel biomarkers for pre-diabetes identified by metabolomics. Molecular Systems Biology. 2012; 8:615 10
California, US
David Wishart | University
Jeremy Nicholson |
of Alberta, Canada
Imperial College
Jan van der Greef |
London, UK
Leiden University,
Leiden, the Netherlands
Science, Dalian, China
Chemistry, Shanghai, China
Metabolomics publications
数据来源：Pubmed 关键词：metabolomics
1.3 Disease Diagnosis
Plantar blood collection
Newborn Genetic Metabolic Disease Screening
Jiao Tong University, Shanghai, China Zhengjiang Zhu | the
Institute of Chemical
Interdisciplinary Research
Physics, Chinese Academy of
Center of Biology and
微生物代谢组学-肠道微生物代谢 组学研究
Contents
Introductions to Metabolomics
Workflow for studies in metabolomics
Microbial Metabolomics
Metabolomics
Part 1: Introductions to Metabolomics
2 0 0 0 D ' 2 # 1 4 0 0 RT: 1 3 . 4 9 A V : 1 N L : 4 . 3 3 E 5 T: F TM S + c E S I Full m s [ 5 0 . 0 0 -1 0 0 0 . 0 0 ]
1 4 4 .9 8 100
95
90
85
80
75
70
65
60
targeted pathway
Advantages
• High-throughput • Easy handling • Capable to discover new
potential biomarkers and cellular mechanism
Disadvantages • Difficult to accurate identification of metabolites
1.4 Precision Nutrition
Zeevi D,etal. Personalized Nutrition by Prediction of Glycemic Responses. Cell. 2015 Nov 19;163(5):1079-94. doi: 10.1016/j.cell.2015.11.001.
593.64
300 400 500 600 700 800 900 1000 m/z
682.58
773.51 860.44 934.04
MS detection
Data analysis
Results reports
Intelligent knife
MasSpec Pen
1 Balog J, etal. Intraoperative Tissue Identification Using Rapid Evaporative Ionization Mass Spectrometry. Sci Transl Med. 2013 Jul 17;5(194):194ra93. 2 Zhang J,etal. Nondestructive tissue analysis for ex vivo and in vivo cancer diagnosis using a handheld mass spectrometry system. Sci Transl Med. 2017 Sep 6;9(406).
Unargeted metabolomics
Targeted metabolomics Metabolic flux
Emily G. Armitage, Coral Barbas. Metabolomics in cancer biomarker discovery: Current trends and future perspectives. Journal of Pharmaceutical and Biomedical Analysis
Metabonomics
Quantitative measurement of the dynamic multiparametric metabolic response of living systems to physiopathological stimuli or genetic modifications. (Jeremy Nicholoson, 1999)
Genomics and proteomics could tell you what might happen, but metabolomics could tell you what has happened.
Janet K. Jansson, etal. A multi-omic future for microbiome studies. NMICROBIOL. 2016 April 26.
essential • Low-throughput
• Accurate identification of metabolites in batches
• High sensitivity • Capable to measure low
abundance metabolites
• Complicated sample preparation
55
50
45 34 50 1 2 8 . 0 7 2 3 3 . 9 2 30 118.09 25 90.06 20
2 2 6 .1 6
15
1 7 4 .0 9
10
5 0
100 200
3 2 4 .8 5
3 1 6 .0 5
4 1 3 .7 8
255.10
341.87 430.81
502.71 5 3 6 .1 7
A targeted metabolomics approach is defined as a
quantitative analysis (concentrations are determined) or semiquantitative analysis(relative intensities are registered) of a few metabolites and/or substrates of metabolic reactions that might be associated to common chemical classes or linked to selected metabolic pathways.
• Low sensitivity • Hard to detect low
abundance metabolites • Dependent of public
database
• Absolute qualitation and quantitation
• Standards essential • Standard curves
• In-house database establishment essential
2.3 Metabolomics Products of Biotree
Untargeted Metabolomics
• Lipidomics • Medical Metabolomics • Pharmaco-Metabolomics • Plant Metabolomics • Microbial Metabolomics • Subcellular Metabolomics • Amino-Metabolomics
Metabolomics
The comprehensive and quantitative analysis of all metabolites of the biological system under study".(Oliver Fiehn, 2001)
1.2 A correlation between the main omics strategies used in systems biology studies
1.1 A Brief History of Metabolomics and ItsRelevance in Systems Biology
Metabolome
The change in the relative concentrations of metabolites as the result of deletion or overexpression of a gene.(Oliver et al., 1998)
1.6 Metabolic signatures of chronic diseases unveiled by metabolomics
Part 2: Workflow for studies in metabolomics
2.1 Workflow for studies in metabolomics
1.2 Current Major Research Labs in Metabolomics
Oliver Fiehn | University of
Gary Siuzdak | the Scripps
California, Davis, US
Research Institute, La Jolla,
• Targeted metabolites measurement
• Clinical biomarkers validation
Widely-targeted Metabolomics
• Metabolites Measurement • Potential biomarkers disovery • Focused on metabolites in the
Xijun Wang |
Heilongjiang University Weijia | Shanghai
Huiru Tang | Fudan Of Chinese Medicine, University, Shanghai, Haerbin, China
Guowang Xu | Dalian
China
An untargeted metabolomics approach is based
primarily on the qualitative or semiquantitative analysis of the largest possible number of metabolites from a diversity of chemical and biological classes contained in a biological specimen.
2.2 Metabolomics Research Caቤተ መጻሕፍቲ ባይዱegories
Technology Objective
Untargeted Metabolomics
• Metabolites detection • Potential biomarkers
disovery
Targeted Metabolomics
1.5 Novel biomarkers for pre-diabetes identified by metabolomics
Rui Wang-Sattler et al., Novel biomarkers for pre-diabetes identified by metabolomics. Molecular Systems Biology. 2012; 8:615 10
California, US
David Wishart | University
Jeremy Nicholson |
of Alberta, Canada
Imperial College
Jan van der Greef |
London, UK
Leiden University,
Leiden, the Netherlands
Science, Dalian, China
Chemistry, Shanghai, China
Metabolomics publications
数据来源：Pubmed 关键词：metabolomics
1.3 Disease Diagnosis
Plantar blood collection
Newborn Genetic Metabolic Disease Screening
Jiao Tong University, Shanghai, China Zhengjiang Zhu | the
Institute of Chemical
Interdisciplinary Research
Physics, Chinese Academy of
Center of Biology and
微生物代谢组学-肠道微生物代谢 组学研究
Contents
Introductions to Metabolomics
Workflow for studies in metabolomics
Microbial Metabolomics
Metabolomics
Part 1: Introductions to Metabolomics
2 0 0 0 D ' 2 # 1 4 0 0 RT: 1 3 . 4 9 A V : 1 N L : 4 . 3 3 E 5 T: F TM S + c E S I Full m s [ 5 0 . 0 0 -1 0 0 0 . 0 0 ]
1 4 4 .9 8 100
95
90
85
80
75
70
65
60
targeted pathway
Advantages
• High-throughput • Easy handling • Capable to discover new
potential biomarkers and cellular mechanism
Disadvantages • Difficult to accurate identification of metabolites
1.4 Precision Nutrition
Zeevi D,etal. Personalized Nutrition by Prediction of Glycemic Responses. Cell. 2015 Nov 19;163(5):1079-94. doi: 10.1016/j.cell.2015.11.001.
593.64
300 400 500 600 700 800 900 1000 m/z
682.58
773.51 860.44 934.04
MS detection
Data analysis
Results reports
Intelligent knife
MasSpec Pen
1 Balog J, etal. Intraoperative Tissue Identification Using Rapid Evaporative Ionization Mass Spectrometry. Sci Transl Med. 2013 Jul 17;5(194):194ra93. 2 Zhang J,etal. Nondestructive tissue analysis for ex vivo and in vivo cancer diagnosis using a handheld mass spectrometry system. Sci Transl Med. 2017 Sep 6;9(406).
Unargeted metabolomics
Targeted metabolomics Metabolic flux
Emily G. Armitage, Coral Barbas. Metabolomics in cancer biomarker discovery: Current trends and future perspectives. Journal of Pharmaceutical and Biomedical Analysis
Metabonomics
Quantitative measurement of the dynamic multiparametric metabolic response of living systems to physiopathological stimuli or genetic modifications. (Jeremy Nicholoson, 1999)
Genomics and proteomics could tell you what might happen, but metabolomics could tell you what has happened.
Janet K. Jansson, etal. A multi-omic future for microbiome studies. NMICROBIOL. 2016 April 26.
essential • Low-throughput
• Accurate identification of metabolites in batches
• High sensitivity • Capable to measure low
abundance metabolites
• Complicated sample preparation
55
50
45 34 50 1 2 8 . 0 7 2 3 3 . 9 2 30 118.09 25 90.06 20
2 2 6 .1 6
15
1 7 4 .0 9
10
5 0
100 200
3 2 4 .8 5
3 1 6 .0 5
4 1 3 .7 8
255.10
341.87 430.81
502.71 5 3 6 .1 7
A targeted metabolomics approach is defined as a
quantitative analysis (concentrations are determined) or semiquantitative analysis(relative intensities are registered) of a few metabolites and/or substrates of metabolic reactions that might be associated to common chemical classes or linked to selected metabolic pathways.
• Low sensitivity • Hard to detect low
abundance metabolites • Dependent of public
database
• Absolute qualitation and quantitation
• Standards essential • Standard curves