骨碎补提取液对体外分离破骨细胞性骨吸收的作用

・论 著・

骨碎补提取液对体外分离破骨

细胞性骨吸收的作用

樊粤光　黄永明　曾意荣　王海彬　黄　荷

(广州中医大学第一附属院骨科　广东　广州　510405)

摘要　目的:研究骨碎补对体外分离破骨细胞性骨吸收的作用。方法:体外分离出破骨细胞,与牛骨磨片共同培养,通过L eica图像分析仪观察破骨细胞所形成骨吸收陷窝的数目与面积,反映破骨细胞性骨吸收情况。结果用SPSS软件包分析。结果:与空白血清组相比,20%骨碎补提取液使骨磨片上形成的吸收陷窝数和面积从(28.05±2.75)个 片和(1239.45±523.21)Λm2减少到(13.20±1.26)个 片和(683.17±341.38)Λm2,空白血清组与20%骨碎补血清组比较差异具有显著性意义(P<0.01),而10%低浓度骨碎补提取液对破骨细胞在骨磨片上形成的吸收陷窝数和面积从(28.05±2.75)个 片和(1239.45±523.21)Λm2减少到(24.15±

1.12)个 片和(823.52±527.18)Λm2,二者比较无显著性意义(P>0.05)。结论:骨碎补提取液对破骨细胞性

骨吸收有抑制作用,但与浓度有关。

关键词　骨碎补　破骨细胞　体外培养　骨吸收

中图分类号:Q813.1 文献标识码:A 文章编号:1005-0205(2003)06-0004-03

Effects of D rynar i a Rh izo m e on Cultiva ted O steocla sts of Bone Resorption i n V itro

Fan Yueguang,H uang Yongm ing,Zeng Y irong,et al

T he F rist A f f ilia ted H osp ita l Guanz huo U n iversity of T CM,Guanz huo,510405,Ch ina

ABSTRACT　Objective:To study effect of Ch inese traditi onal m edicine-D rynaria rh izom e on cu ltivated o s2 teoclasts of bone reso rp ti on in vitro.M ethods:Cu ltivated o steoclasts from long bones of w istar rats,and even ly inocu lated on24w ell cu ltivaed p lates w h ich pu t cow’s tib ial slices in advance.T he area and num ber of the reso rp ti on p its that reflected effects of D rynaria rh izom e on cu ltu red o steoclasts of bone reso rp ti on in vit2 ro w ere determ ined w ith the L eica Q uan ti m en t500system.T he resu lts w ere analysed by statistical package

养状况。同时又可促进成纤维细胞、成肌细胞增殖,使损伤组织迅速修复,达到愈合。因此,以活血化瘀,消肿止痛为主要功效的复方西红花膏对损伤组织修复作用与碱性成纤维细胞生长因子的活性密切相关。

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