大豆结荚习性、荚色和种皮色相关野生片段分析

A bstract:Stem termination(ST),pod color(PC)and seed coat color(SCC)are important morphological traits,w hich are related to evolution in soybean.B y using a wild soybean(Glycine soja Sieb et Zucc.)chrom osom e segm ent substitution line(CSSL) population,designated as SojaCSSLPl com posed of 151 lines,one ST,three PC and two SCC wild segm ents/alleles were detected based on the com parison of different CSSL groups with a sam e phenotype on the respective trait.A m ong them ,five wild alleles/segm ents identified in this study were corresponding to Dt1,L2,L1,G and i,respectively,w hich indicated that there existed allele differentiation happened between wild and cultivated soybean as well as between cultivated soybeans on these loci/segm ents and that the genes/segm ents involved with dom estication and evolution.The wild segm ent of Satt273 for PC might be a novel gene/segm ent,w hich needed further verification.The identification of the genes/segm ents provide basic materials for cloning the wild alleles,studying the wild allele function and recom bination of the wild alleles/segm ents. K eywords:W ild soybean;Chrom osom e segm ent substitution line;Pod color;Stem termination;Seed coat color
与结荚习性、荚色和种皮色相关的1、3和2个野生片段（基因）。其中，5个野生片段（基因）分别与前人在栽培豆中检 测到的无限结荚Dt1、荚色L1、荚色L2、绿种皮G 和黑种皮i基因相对应，说明野生大豆与栽培大豆间、栽培大豆 与栽培大豆间在这些片段上均存在等位变异分化，是与大豆进化相关的基因/片段。另一个与荚色相关的Satt273 野 生片段能使大豆表现黑荚，可能是本研究的新发现，但还需进一步验证。 关键词：野生大豆；染色体片段代换系；荚色；结荚习性；种皮色
Id e n tifica tio n of Wild Seg men ts Asso c ia ted w ith S tem a n d Seed Co a t Co lo r in S o y b e a n
wk.baidu.com
T e r min a tio n ，P o d Co lo r,
W A N G Wu-B in,H E Qing-Y uan,Y A N G H ong-Y an,X IA N G Shi-H ua,X IN G Guang-N an,Z H A O Tuan-Jie*, and G A I Jun-Y i*
大豆结荚习性、荚色和种皮色相关野生片段分析
王吴彬 何庆元 杨红燕 向仕华 邢光南 赵团结* 盖钧镒*
南京农业大学大豆研究所/国家大豆改良中心/农业部大豆生物学与遗传育种重点实验室（综合）/作物遗传与种质创新国家重点实 验室，江苏南京 210095
摘 要：结荚习性、荚色和种皮色是大豆的重要形态性状，与进化密切相关。利用由151个家系组成的野生大豆 （Glycine soja Sieb et Zucc）染色体片段代换系（CSSL）群体（SojaCSSLP1），通过不同表型CSSL组间比对，分别检测到
大豆[Glycine max（L.）Merr.]原产于中国，是当 今世界最重要的植物蛋白与植物油来源。野生大豆 （G. soja Sieb.et Zucc.）是栽培大豆的野生祖先，具有 无限结荚、黑荚及黑种皮等典型特征。
大豆结荚习性（stem termination,ST），分为有 限、亚有限和无限3种类型。根据SoyBase（http：//
作物学报 ACTA AGRONOMICA SINICA 2013，39（7）：1155-1163 ISSN 0496-3490；CODEN TSHPA9
D O I：10.3724/SP.J.1006.2013.01155
http：//www.chinacrops.org/zwxb/ E-mail:xbzw@chinajournal.net.cn
Soybean Research Institute/National Center for Soybean Improvement/MOA Key Laboratory for Biology and Genetic Improvement of Soybean (General)/National Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China