3-Indoleacetic_acid_HNMR_23874_MedChemExpress

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美国 Gibco 最小必要基质 MEM 产品说明书

Product Information MINIMUM ESSENTIAL MEDIUM EAGLED-VALINE MODIFICATIONProduct Number M7395Storage Temperature 2-8°CProduct DescriptionMinimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle’s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks’ or Earle’s salts has broadened the usefulness of this medium. The formulation has been further modified by optional elimination of calcium to permit growth of cells in suspension culture.MINIMUM ESSENTIAL MEDIUM EAGLE, Product No. M 7395 is one of the cell culture media available from Sigma. The selection of a nutrient medium is strongly influenced by 1] type of cell, 2] type of culture [monolayer, suspension, clonal] and 3] degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.Components g/L Calcium Chloride•2H2O0.265 Magnesium Sulfate (anhydrous)0.09767 Potassium Chloride0.4 Sodium Chloride 6.8 Sodium Phosphate Monobasic0.122 (anhydrous)L-Arginine•HCl0.126L-Cystine•2HCl0.0313 L-Glutamine0.292L-Histidine•HCl•H2O0.042L-Isoleucine0.052L-Leucine0.052L-Lysine•HCl0.0725 L-Methionine0.015L-Phenylalanine0.032L-Threonine0.048L-Tryptophan0.01L-Tyrosine•2Na•2H2O0.0519D-Valine0.092 Choline Chloride0.001Folic Acid0.001myo-Inositol0.002 Niacinamide0.001D-Pantothenic Acid (hemicalcium)0.001 Pyridoxal•HCl0.001 Riboflavin0.0001 Thiamine•HCl0.001 Glucose 1.0 Phenol Red•Na0.011Precautions and DisclaimerREAGENTFor In Vitro Diagnostic UsePreparation InstructionsPowdered media are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing a concentrated solution of medium is not recommended as precipitates may form. Supplements can be added prior to filtration or introduced aseptically to sterile medium. The nature of the supplement may affect storage conditions and shelf life of the medium.1.Measure out 90% of final required volume ofwater. Water temperature should be 15-20°C.2.While gently stirring the water, add thepowdered medium. Stir until dissolved. Do NOTheat.3.Rinse original package with a small amount ofwater to remove all traces of powder. Add tosolution in step 2.4.To the solution in step 3, add 2.2 g sodiumbicarbonate or 29.3 ml of sodium bicarbonatesolution [7.5%w/v] for each liter of final volumeof medium being prepared. Stir until dissolved.5.While stirring, adjust the pH of the medium to0.1-0.3 pH units below the desired pH since itmay rise during filtration. The use of 1N HCl or1N NaOH is recommended.6.Add additional water to bring the solution tofinal volume.7.Sterilize immediately by filtration using amembrane with a porosity of 0.22 microns.8.Aseptically dispense medium into sterilecontainer.Storage/StabilityStore the dry powdered medium at 2-8°C under dry conditions and liquid medium at 2-8°C in the dark. Deterioration of the powdered medium may be recognized by any or all of the following: [1] color change, [2] granulation/clumping, [3] insolubility. Deterioration of the liquid medium may be recognized by any or all of the following: [1] pH change, [2] precipitate or particulate matter throughout the solution, [3] cloudy appearance [4] color change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.ProcedureWater for tissue culture use [W-3500]Sodium Bicarbonate [S-5761] orSodium Bicarbonate Solution, 7.5% [S-8761]1N Hydrochloric Acid [H-9892]1N Sodium Hydroxide [S-2770]Medium additives as requiredProduct ProfileAppearance off-white powder Moisture content 2.0% Solubility clear solution at 1x concentrationpH at room temperature 5.8 ± 0.3 [without sodium bicarbonate]pH at room temperature 7.5 ± 0.3 [with sodium bicarbonate]Osmolality250 mOsm/kg H2O ± 5% [without sodium bicarbonate]Osmolality290 mOsm/kg H2O ± 5% [with sodium bicarbonate]Amino Acid Analysis Analysis has confirmedby HPLC that amino acids are present atconcentrations consistent withthe formula.Key Element Analysis Analysis has confirmed that by ICAP key elements are present atconcentrations consistent withthe formula.BIOLOGICAL PERFORMANCE CHARACTERISTICS Biological performance is assessed using an appropriate cell line(s). Growth studies are carried through 2 subculture generations. Cells are counted and growth is plotted as a logarithmic function of time in culture. Seeding efficiencies, doubling time, and final cell densities are determined. During the testing period cultures are examined microscopically for atypical morphology and evidence of cytotoxicity. Test results are available upon request.References1.Eagle, H. et al (1956) myo-Inositol as anEssential Growth Factor for Normal andMalignant Human Cells in Tissue Culture.J.Biol. Chem. 214, 845-847.2.Eagle, H.(1976) Media for Animal Cell Culture.Tissue Culture Association Manual. 3, 517-520.3.Eagle, H. (1959). Amino Acid Metabolism inMammalian Cell Cultures. Science. 130, 432-437.4.Eagle, H. (1955) Nutrition Needs of MammalianCells in Culture. Science. 122, 501.5.Gilbert, S.F. and Migeon, B.R. (1975) D-valineas a selective agent for normal human androdent epithelial cells in culture. Cell. 5, 11-17.7H027Sigma brand products are sold through Sigma-Aldrich, Inc.Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side ofthe invoice or packing slip.。

超高效液相色谱-串联质谱法测定人血浆中精氨酸及衍生物含量

超高效液相色谱-串联质谱法测定人血浆中精氨酸及衍生物含量田晔;江骥;胡蓓;薛金萍;王洪允【摘要】建立了超高效液相色谱-串联质谱(UPLC-MS/MS)法同时测定使用艾普拉唑后人血浆中二甲基精氨酸(ADMA)、对称二甲基精氨酸(SDMA)、单甲基精氨酸(NMMA)、瓜氨酸(Cit)和L-精氨酸(L-Arg)的浓度.采用HILIC亲水相互作用色谱和非衍生化的蛋白沉淀法进行分离分析,色谱柱选取Waters Atlantic HILIC柱(2.1 mm×50 mm×3μm),流动相由乙腈(含0.5％乙酸和0.025％三氟乙酸)-水(含0.5％乙酸和0.025％三氟乙酸)(85:15,v/V)组成,流速0.25 mL/min.采用多反应离子监测(MRM)模式,以电喷雾离子源(ESI)正离子方式检测.结果显示,ADMA、SDMA、NMMA、L-Arg和Cit的线性关系良好,相关系数r均大于0.994 0;ADMA、SDMA和NMMA的线性范围为0.1～5 mmol/L,L-Arg和Cit的线性范围为10～250 mmol/L;5种氨基酸的日内、日间精密度均小于15％,准确度在85％～115％之间.该方法快速、简便、灵敏,可为相关疾病的临床诊断提供一种高效的检测手段.【期刊名称】《质谱学报》【年(卷),期】2016(037)005【总页数】7页(P446-452)【关键词】超高效液相色谱-串联质谱(UPLC-MS/MS);艾普拉唑;蛋白沉淀法;亲水性色谱【作者】田晔;江骥;胡蓓;薛金萍;王洪允【作者单位】福州大学化学学院,福建省功能材料工程研究中心,福建省光动力治疗药物与诊疗工程技术研究中心,福建福州350108;中国医学科学院北京协和医院临床药理中心,北京100730;中国医学科学院北京协和医院临床药理中心,北京100730;中国医学科学院北京协和医院临床药理中心,北京100730;福州大学化学学院,福建省功能材料工程研究中心,福建省光动力治疗药物与诊疗工程技术研究中心,福建福州350108;中国医学科学院北京协和医院临床药理中心,北京100730【正文语种】中文【中图分类】O657.63一氧化氮是人体重要的信使分子，L-精氨酸(L-Arg)在一氧化氮全酶(NOS)的催化下，产生一氧化氮(NO)和瓜氨酸(Cit)[1-2]。

分子生物学词汇(中英文对照表 )

华中科技大学学术期刊分类目录(T-D)_最新权威版

24.755 23.917 23.654 23.565 23.333 23.194 22.929 22.864 22.864 22.864 22.490 22.345 22.333 21.757 21.543 21.543 20.833 20.761 20.614 19.966 19.795 19.547 19.352 18.571 18.571 18.038 17.983 17.983 17.949 17.689 17.689 17.689 17.436 17.313 17.215 16.417 16.238 16.179 16.008 16.008 15.766 15.575 15.518 15.389 15.389 15.389 15.333 15.333 15.280 15.280 15.265 15.253 15.251 15.202
ONCOLOGY CLINICAL NEUROLOGY PLANT SCIENCES BIOCHEMICAL RESEARCH METHODS ASTRONOMY & ASTROPHYSICS MATERIALS SCIENCE, MULTIDISCIPLINARY PHYSICS, MULTIDISCIPLINARY BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY MEDICINE, RESEARCH & EXPERIMENTAL MICROBIOLOGY PHARMACOLOGY & PHARMACY PHYSICS, PARTICLES & FIELDS CHEMISTRY, MULTIDISCIPLINARY PHARMACOLOGY & PHARMACY TOXICOLOGY CHEMISTRY, MULTIDISCIPLINARY CELL BIOLOGY NEUROSCIENCES INFECTIOUS DISEASES IMMUNOLOGY PHYSIOLOGY PHYSICS, MULTIDISCIPLINARY BEHAVIORAL SCIENCES NEUROSCIENCES ONCOLOGY CELL BIOLOGY DEVELOPMENTAL BIOLOGY ECOLOGY CHEMISTRY, MULTIDISCIPLINARY MAபைடு நூலகம்ERIALS SCIENCE, MULTIDISCIPLINARY NANOSCIENCE & NANOTECHNOLOGY GENETICS & HEREDITY MICROBIOLOGY MEDICINE, GENERAL & INTERNAL MICROBIOLOGY ASTRONOMY & ASTROPHYSICS MATERIALS SCIENCE, MULTIDISCIPLINARY BEHAVIORAL SCIENCES NEUROSCIENCES NEUROSCIENCES PSYCHOLOGY CLINICAL NEUROLOGY ECOLOGY EVOLUTIONARY BIOLOGY GENETICS & HEREDITY CHEMISTRY, PHYSICAL PHYSICS, CONDENSED MATTER BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY PSYCHOLOGY MEDICINE, GENERAL & INTERNAL NEUROSCIENCES CARDIAC & CARDIOVASCULAR SYSTEMS

非酒精性脂肪性肝病代谢组学研究进展

机制尚未完全明确，１９９８年Ｄａｙ等［１２］提出“二次打击”学说。开。同时ＮＡＦＬＤ肝硬化患者与酒精性肝硬化患者也可有效区
随后Ｔｉｌｇ等［１３－１４］提出“多重平行打击”理论，包括遗传因素、分开（ＡＵＣ＝０．８３）。他们认为此方法可作为区分ＮＡＦＬＤ纤维
ＩＲ、氧化应激、脂毒性、慢性炎症、纤维化、免疫和肠道菌群等，化程度及诊断的无创生物标志物，且可以显著减少对肝活检的
黄酯和１３－ｃｉｓＲＡ呈正相关。他们在人类组织中首次检测到验证；单不饱和ＴＡＧ的增加可能是ＮＡＦＬＤ和ＣＨＢ患者ＮＡＳＨ
ａｔＲＡ的活性代谢物４－ｏｘｏ－ａｔＲＡ，表明这种类维生素Ａ可能的特异性标志物。
有助于人体类维生素Ａ的信号传导。肝脏维生素Ａ的稳态平２．３代谢组学对ＮＡＦＬＤ药物作用与疗效研究的推动作用
录组学、蛋白质组学为代表的系统生物学技术提供了新的技术展的新学科，代谢组学较为全面的展示了机体的代谢结果，为
与思路。区别于其他组学技术，以内源性小分子代谢物为研究临床医学提供了新的技术和方法。
对象的代谢组学可以很好的揭示机体变化的最终代谢结果。因２非酒精性脂肪性肝病（ＮＡＦＬＤ）
收基作ＤＯ稿金者Ｉ：日项简１０期目介．３：：：９１２上栾研６７０９）２海究雨／０ｊ．中婷－ｉｓ医１ｓ（ｎ１药．１－１９大０９０００６学１—；修－附）５回，属２女５日第６，．期七主２０：人２要２１０民．２从００医４事－．院０１慢４２人７－性才１肝７培病养计的划基（础ＸＸ与２０临１９床－通信作者：顼志兵，ｘｚｂ６１６０＠１６３．ｃｏｍ
和遗传易感密切相关的代谢应激性肝损伤，包括非酒精性单纯１代谢组学概述
性肝脂肪变（ＮＡＦＬ）、非酒精性脂肪性肝炎（ＮＡＳＨ）、肝硬化和１．１代谢组学含义代谢组学最初于１９９９年由Ｎｉｃｈｏｌｓｏｎ

黄芩素通过调节HIF-1α

黄芩素通过调节HIF -1α/VEGF 信号通路抑制类风湿关节炎大鼠的炎症反应和病理性血管生成*杜红丽1，张晨宇1，赵清2△［1河南中医药大学第五临床医学院（郑州人民医院）风湿免疫科，河南郑州450053；2河南大学淮河医院风湿免疫科，河南开封475099］［摘要］目的：探讨黄芩素（BA ）调节缺氧诱导因子1α（HIF -1α）/血管内皮生长因子（VEGF ）信号通路对类风湿关节炎（RA ）大鼠炎症反应和病理性血管生成的影响。

方法：按照随机数字表法将SD 大鼠分为对照（control ）组、模型（model ）组、低剂量（10mg/kg ）BA （BA -L ）组、高剂量（30mg/kg ）BA （BA -H ）组、雷公藤多苷片（TWP ；6.25mg/kg ）组和BA -H+HIF -1α激动剂二甲基草酰甘氨酸（DMOG ；40mg/kg ）组，每组12只。

除control 组外，其它组大鼠均采用II 型胶原蛋白-完全弗氏佐剂法诱导RA 大鼠模型。

第2次免疫24h 后开始给药处理，每天给药一次，持续4周。

检测大鼠在给药第0、7、14和28天时的足趾肿胀度，计算关节炎指数；计算大鼠胸腺和脾脏指数；HE 染色检测大鼠踝关节滑膜组织病理损伤；ELISA 法检测大鼠踝关节滑膜组织中肿瘤坏死因子α（TNF -α）和白细胞介素6（IL -6）水平；免疫组化检测大鼠踝关节滑膜组织中VEGF 和VEGF 受体2（又称激酶插入域受体，KDR ）表达；Western blot 检测各组大鼠踝关节滑膜组织中HIF -1α和VEGF 蛋白表达。

结果：与control 组比较，model 组大鼠踝关节滑膜组织病理损伤严重，足趾肿胀度、关节炎指数、胸腺和脾脏指数，以及滑膜组织TNF -α、IL -6、VEGF 、KDR 、HIF -1α和VEGF 水平均显著升高（P <0.05）；与model 组比较，BA -L 组、BA -H 组和TWP 组对应指标变化趋势与上述相反（P <0.05）；BA -H 组与TWP 组比较，上述指标变化差异无统计学意义（P >0.05）；DMOG 减弱了BA -H 对RA 大鼠炎症反应和病理性血管生成的抑制作用。

尚玺胶囊的抗炎作用及其对血清IL-1β、TNF-α和炎症组织中PGE2的影响

6人参研究GINSENG RESEARCH 2021年第6期IL-1β、TNF-αPGE 2韩鸾玮,牛雪妮,吕重宁,路金才*(沈阳药科大学中药学院·辽宁沈阳·110016)摘要:,IL-1β、TNF-αPGE 2。

,。

,(Superoxide dismutase,SOD)、(Malondialdehyde,MDA);(ELISA)1β(IL-1β)、α(TNF-α)E2(PGE 2)。

,,14。

300mg ·kg -1,2(7.58%),(8.30%);,MDA、IL-1βTNF-α、SOD ,PGE 2。

300mg/kg ,IL-1β、TNF-α、PGE 2。

关键词:;;TNF-α;PGE 2、、、、、,。

(Resveratrol,Res),,(),:、、、、、、、、[1-7],,。

,[8]。

:、5-,[9]。

,,。

1实验材料1.1(:81K1556)Sigma;(:190304);(:190124)(Abstract:Objective To evaluate the anti-inflammatory activity of Shang-Xi Capsule (SXC)and explore its efftct onIL-1β,TNF-αin serum,and PGE 2in inflammatory tissues.Methods The carrageenan-induced paw edema experiment was performed to evaluate the anti-inflammatory effect of SXC.And serum superoxide dismutase (SOD),malondialdehyde (MDA),IL-1β,TNF-αand PGE 2were measured.Results Compared with the control group,SXC at the dosage of 300mg·kg -1significantly decreased the swelling of inflammatory paw within 1to 4hours after the administration,and the inhibition rate of paw swelling reached a peak (7.58%)after 2hours,which was close to that of resveratrol (8.30%).In addition,it can reduce the content of PGE 2and NO in inflammatory tissue and the content of NO,MDA,IL-1β,and TNF-αin serum.Conclusion SXC can reduce the acute inflammation induced by carrageenan.Its mechanism of action may be associated with reducing PGE 2synthesis in inflammatory tissue,inhibiting the formation of lipid peroxide products in tissue inflammation,and reducing the content of inflammatory factor TNF-α.Keywords:Shang-Xi Capsule;anti-inflammation;TNF-α;PGE 2Study on Anti-inflammatory Activity of Shang-Xi Capsule and Effect on IL-1β,TNF-αin serum,and PGE 2in inflammatory tissuesHAN Luan-wei,NIU Xue-ni,LV Chong-ning,LU Jin-cai*(School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China)作者简介:,,,。

GC测定盐酸普拉克索中三乙胺残留量

ABSTRACT: OBJECTIVE To determine the triethylamine in pramipexole hydrochloride by GC. METHODS The residual triethylamine was determined by HS-GC with Agilent-INNOWAX capillary column(30 m×0.32 mm, 0.5 μm) and FID detector. The carrier gas was nitrogen and the flow rate was 4.0 mL·min1. The temperature of the injection port was maintaining at 250 ℃ and the same of detector. The oven introduced sequential increasing of temperature programing. The initial column temperature was 50 ℃, then raised the temperature to 150℃ at a rate of 10 ℃·min1, and maintained it at 150 ℃ for 5 min, then raised the temperature to 220 ℃ at a rate of 40 ℃·min1, and maintained it for 5 min. The headspace oven was set at a temperature of 80 ℃ for 30 min. The solvent was 20% sodium hydroxide solution. The triethylamine was quantified external standard. RESULTS The calibration shows a good linearity with the range of 0.31712.68 μg·mL1 for triethylamine. The average recovery was 97.9%(n=9). The precision was 4.18%(n=9). CONCLUSION This method is accurate, reliable and sensitive for the

重组贻贝粘蛋白的表征及功效评价

生物技术进展 2023 年第 13 卷第 4 期 596 ~ 603Current Biotechnology ISSN 2095‑2341研究论文Articles重组贻贝粘蛋白的表征及功效评价李敏，魏文培，乔莎，郝东，周浩，赵硕文，张立峰，侯增淼 *西安德诺海思医疗科技有限公司，西安 710000摘要：为了推进重组贻贝粘蛋白在医疗、化妆品领域的应用，对大肠杆菌规模化发酵及纯化生产获得的重组贻贝粘蛋白进行了表征及功效评价。

经Edman 降解法、基质辅助激光解吸电离飞行时间质谱、PITC 法、非还原型SDS -聚丙烯酰胺凝胶电泳法、凝胶法、改良的Arnow 法对重组贻贝粘蛋白进行氨基酸N 端测序、相对分子量分析、氨基酸组成分析、蛋白纯度分析、内毒素含量测定、多巴含量测定；通过细胞迁移、斑马鱼尾鳍修复效果对重组贻贝粘蛋白进行功效评价。

结果显示，获得的重组贻贝粘蛋白与理论的一级结构一致，蛋白纯度达95%以上，内毒素<10 EU ·mg -1,多巴含量大于5%；重组贻贝粘蛋白浓度为60 μg ·mL -1时能够显著促进细胞增殖的活性（P <0.01）；斑马鱼尾鳍面积样品组与模型对照组相比极显著增加（P <0.001）。

研究结果表明，重组贻贝粘蛋白具有显著的促细胞迁移和修复愈合的功效，具备作为生物医学材料的潜质。

关键词：贻贝粘蛋白；基因重组；生物材料；表征；功效评价DOI ：10.19586/j.20952341.2023.0021 中图分类号：S985.3+1 文献标志码：ACharacterization and Efficacy Evaluation of Recombinant Mussel Adhesive ProteinLI Min ， WEI Wenpei ， QIAO Sha ， HAO Dong ， ZHOU Hao ， ZHAO Shuowen ， ZHANG Lifeng ，HOU Zengmiao *Xi'an DeNovo Hith Medical Technology Co.， Ltd ， Xi'an 710000， ChinaAbstract ：In order to promote the application of recombinant mussel adhesive protein in the medical and cosmetics field ， the recombi⁃nant mussel adhesive protein obtained from scale fermentation and purification of Escherichia coli was characterized and its efficacy was evaluated. Amino acid N -terminal sequencing ， relative molecular weight analysis ， amino acid composition analysis ， protein purityanalysis ， endotoxin content ， dihydroxyphenylalanine （DOPA ） content of recombinant mussel adhesive protein were determined by the following methods ： Edman degradation ， matrix -assisted laser desorption ionization time -of -flight mass spectrometry （MALDI -TOF -MS ）， phenyl -isothiocyanate （PITC ）， nonreductive SDS -polyacrylamide gel electrophoresis （SDS -PAGE ）， gel method ， modified Ar⁃now. The efficacy of recombinant mussel adhesive protein was evaluated by cell migration and repairing effect of zebrafish tail fin. Re⁃sults showed that the obtained recombinant mussel adhesive protein was confirmed to be consistent with the theoretical primary structure ， protein purity of more than 95%， endotoxin <10 EU ·mg -1， DOPA content above 5%. When the recombinant mussel adhesive protein concentration was 60 μg ·mL -1， the effect of promoting cell proliferation was the most obvious ， and it had very significant activity （P <0.01）. The caudal fin area of zebrafish in sample group was significantly increased compared with model control group （P <0.001）. The results indicated that recombinant mussel adhesive protein can promote cell migration and repair healing and has the potential to be used as biomedical materials.Key words ：mussel adhesive protein ； gene recombination ； biological materials ； representation ； efficacy evaluation贻贝粘蛋白（mussel adhesive protein ， MAP ）也称作贻贝足丝蛋白（mussel foot protein ，Mfps ），收稿日期：2023⁃02⁃24；接受日期：2023⁃03⁃31联系方式：李敏 E -mail:*******************;*通信作者侯增淼 E -mail:***********************.cn李敏，等：重组贻贝粘蛋白的表征及功效评价是海洋贝类——紫贻贝（Mytilus galloprovincalis）、厚壳贻贝（Mytilus coruscus）、翡翠贻贝（Perna viri⁃dis）等分泌的一种特殊的蛋白质，贻贝中含有多种贻贝粘蛋白，包括贻贝粘蛋白（Mfp 1～6）、前胶原蛋白（precollagens）和基质蛋白（matrix proteins）等［1］。

天冬酰胺合成酶通过促进β-catenin核转位驱动胆管癌转移

天冬酰胺合成酶通过促进β-catenin 核转位驱动胆管癌转移*褚珍珍1，2，周栩萱1，2，刘力豪1，张鲍欢3△，姚楠1，2△（1暨南大学基础医学院病理生理学系，广东广州 510632；2国家中医药管理局病理生理科研实验室，广东广州510632；3暨南大学基础医学院形态学实验教学中心，广东广州 510632）［摘要］目的：检测天冬酰胺合成酶（ASNS ）在胆管癌（CCA ）中的表达情况，探讨ASNS 在CCA 转移中的作用及其机制。

方法：通过公共数据库分析各肿瘤组织中ASNS 的mRNA 表达；收集CCA 患者病理组织（n =27），构建硫代乙酰胺诱导的大鼠自发CCA 模型和左中位胆管结扎联合二乙基亚硝胺诱导的小鼠自发CCA 模型，通过免疫组化、Western blot 和免疫荧光法检测ASNS 蛋白表达。

采用CCK8、划痕和Transwell 实验检测ASNS 对人CCA 细胞HuCCT1和HCCC -9810增殖、迁移和侵袭的影响。

构建ASNS 稳定敲减的CCA 细胞株HuCCT1shNC 、HuCCT1shASNS 、HCCC -9810shNC 和HCCC -9810shASNS ，通过肝原位种植和尾静脉注射研究ASNS 对CCA 细胞肝内生长和肺转移的影响。

利用公共数据库富集与ASNS 相关的信号通路，并用免疫荧光和Western blot 验证相关分子机制。

结果：无论在人或动物CCA 组织中，ASNS 表达水平均高于癌旁组织（P <0.01）。

ASNS 以酶活性非依赖性方式促进CCA 细胞HuCCT1和HCCC -9810的增殖、迁移与侵袭。

生物信息学分析显示，β-catenin 在ASNS 高表达的CCA 组织中富集，ASNS 通过促进β-catenin 核转位，启动CCA 细胞上皮-间充质转化（EMT ）。

β-catenin 抑制剂XAV -939可显著抑制CCA 细胞的侵袭与迁移。

抗抑郁药---盐酸维拉佐酮

2014.10.20
前言 • MDD治疗的主要药物种类
• 第一代经典抗抑郁药：主要包括单胺氧化酶抑制剂（maoi）和三环类抗抑郁药（tca）。
• 第二代新型抗抑郁药：以选择性五羟色胺（5-ht）再摄取抑剂为主。 • 盐酸维拉佐酮（vilazodone hydrochloride）是首个吲哚烷基胺类新型抗抑郁药。
2014.10.20
药物背景 • 安全性
• 盐酸维拉佐酮在8个临床试验共计2 177例MDD患者的临床研究中显示具有良好的耐受性和安全性。在与安慰剂，对照的Ⅲ临床研究中，因不良反应导致盐酸维拉佐酮治疗组中止治疗的患者占7．1％，安慰剂对照组为3．2％，治疗组导致停药的常见不良反应主耍为恶心(1． 3％)和腹泻(1．2％) 。 • 由于抗抑郁药可增加儿童、青少年和l 8～24岁年轻人服药初期自杀想法和自杀行为的风险，因此以上患者要慎用。 • 维拉唑酮在治疗终止时，尤其突然终止时，会出现戒断症状(烦躁不安、易怒、眩晕、感觉障碍、意识模糊等症状)。
2014.10.20
报告框架
• 1.药物背景
• 2.专利介绍 • 3.合成路线
Viibryd（盐酸维拉佐酮）
2014.10.20
药物背景
• • • • • • • • • • • • • • • • 通用名称：维拉佐酮（Vilazodone）商品名：Viibryd 原研公司：德国Merck KGaA 基本专利： DE19934333254 优先权：1993 年9 月30 日相关中国专利： CN94116585 类别：抑郁症治疗药化合物类型：新分子实体 5-［4-［4-( 5-cyano-1H-indol-3-yl) butyl］-1（New molecular entity） piperazinyl］-2-benzofurancarboxamide 分子式：C26H27N5O2 ·HCl hydrochloride 相对分子质量：477.99 CAS 号：163521-08-2 适应症：重度抑郁症（MDD）化学名： 5-［4-［4-( 5-氰基-1H-吲哚-3-基) 丁基］-1-哌嗪基］-2-苯并呋喃草酰胺盐酸盐; 获批单位：Trovis 制药有限责任公司批准日期：2011 年1 月21 日

迷迭香酸对过氧化氢处理下的皮肤黑色素瘤的抗氧化作用(原文翻译)

迷迭香酸（罗丹酚酸）对H2O2处理过的皮肤黑色素瘤细胞的抗氧化作用Sun Mi Yoo1 and Jeong Ran Kang2*1.韩国光州500-741号东冈大学美容系2.韩国首尔143-701号建国大学生物工程系2009.2.6收到 2009.4.17接收本学科旨在检测迷迭香酸对人工孵育的皮肤黑色素瘤细胞在ROS下的抗氧化作用。

通过XTT比色法，以细胞毒性和抗氧化作用来分析细胞粘附活性，DPPH自由基清除活性以及H2O2处理1-10h和未经处理的两种情况下乳酸脱氢酶的活性。

用20-110 μM 的H2O2处理皮肤黑色素瘤细胞5-7h后，细胞活性的降低呈剂量和时间依赖性。

通过XTT比色法测得H2O2的半抑制浓度（IC50 ）为90μM。

同时H2O2增强了LDH细胞的剂量依赖性。

用50-90μM的H2O2处理8h后测得LDH50为60 μM H2O2。

迷迭香酸能增强细胞活性和DPPH自由基清除活性，降低乳酸盐脱氢酶的活性。

细胞的H2O2处理证实了对人工孵育的皮肤黑色素瘤细胞的强抗氧化作用。

通过H2O2的处理，迷迭香酸能在细胞内能增强细胞活性和DPPH 自由基清除活性，降低乳酸盐脱氢酶的活性。

这被认为是迷迭香酸对ROS（ROS）如H2O2的抗氧化作用。

Key words：DPPH-radical scavenging, LDH, rosmarinic acid, XTT assay关键字：DPPH自由基清除活性，乳酸脱氢酶，迷迭香酸，XTT比色法据研究发现，ROS通过氧化应激对细胞的损伤和一些脑部疾病比如帕金森症或心脏疾病例如心肌梗塞之间有很大的关联[Difazio et al., 1992; Delanty and Dichter, 1998].尤其是研究人员认为ROS是皮肤老化的一个主要的因素后，一直试图从ROS方面研究衰老。

[Yokozawa et al., 1998].据研究表明，ROS的氧化应激会通过萎缩细胞引起各种疾病，例如超氧自由基、H2O2（H2O2）或羟基自由基的巯基蛋白反应中断酶的活性，破坏脱氧RMA（DNA）或RMA（RNA），诱导细胞膜脂质过氧化。

氰基硼氢化钠还原胺化京尼平合成拟生物碱与活性

氰基硼氢化钠还原胺化京尼平合成拟生物碱与活性秦杰琛 1），曾小娟 1），张韶湘 1），张晓梅 2），刘鑫洋 1），邹　澄 1），赵　庆 2）（1）昆明医科大学药学院暨云南省天然药物药理重点实验室，云南昆明　650500；2）云南中医药大学中药学院，云南昆明　650500）[ 摘要 ] 目的　以京尼平苷为原料通过还原胺化反应合成拟生物碱的方法。

方法　京尼平与胺类化合物在氰基硼氢化钠存在下进行还原反应：京尼平与芳基乙胺的甲醇溶液混合后，加入过量氰基硼氢化钠，放置室温下反应3d，产物经石油醚-异丙醇-二乙胺，石油醚-乙酸乙酯等洗脱分离。

结果　合成共得到9个拟生物碱并对部分拟生物碱进行活性筛选，找到治疗Ⅱ型糖尿病的PTP1B 抑制剂。

结论　部分受试化合物对PTP1B 有抑制作用。

一系列活性衍生物的获得为化合物结构及其生物活性间的构效关系研究打下了基础，有利于寻找具有更高活性的PTP1B 抑制剂。

[ 关键词 ] 京尼平；拟生物碱；还原胺化；抗PTP1B 活性[ 中图分类号 ] R284.1 [ 文献标志码 ] A [ 文章编号 ] 2095 − 610X （2021）02 − 0018 − 05Reductive Amination of Genipin with NaBH 3CN toSynthesize Alkaloid-likes and BioactivityQIN Jie-chen 1），ZENG Xiao-juan 1），ZHANG Shao-xiang 1），ZHANG Xiao-mei 2），LIU Xin-yang 1），ZOU Cheng 1），ZHAO Qing 2）（1） School of Pharmaceutical Science & Yunnan Key Laboratory of Pharmacology for Natural Products ，Kunming Medical University ，Kunming Yunnan 650500; 2） Faculty of Pharmacy ，Yunnan University of Chinese Medicine ，Kunming Yunnan 650500，China ）［Abstract ］ Objective To explore a method for the synthesis of alkaloid-likes from Genipin by reductive amination is reported. Methods The reduction of Genipin and amines in the presence of sodium cyanoborohydride：after the methanol solution of Genipin and arylethylamine was mixed，excessive sodium cyanoborohydride was added and the reaction was kept at room temperature for 3 days. The product was eluted and separated on silica gel by petroleum ether-isopropyl alcohol-diethylamine and petroleum ether-ethyl acetate. Results Nine alkaloid-likes were synthesized. Some alkaloid-likes were screened for inhibition activity of PTP1B enzyme for Ⅱ diabetes treatment. Conclusions All of the tested compounds have a certain inhibitory effect on PTP1B. The acquisition of a series of active derivatives has laid a foundation for the study of the structure-activity relationship between the compounds and their bioactivities，so as to facilitate the search for more active PTP1B inhibitors.［Key words ］ Genipin；Alkaloid-likes；Reductive amination；Inhibition activity against PTP1B目前，通过发现先导化合物是现代新药研发的重要出发点，研究者对具有特定生物活性的先导化合物，利用生物、化学方法进行结构修饰，从而减少化合物毒副作用，提高化合物的活性，增强其生物利用度，最终找到一些作用效果明显，副作用少的新药应用于临床治疗。

3-磷酸甘油酸脱氢酶在肿瘤中的研究进展

3-磷酸甘油酸脱氢酶在肿瘤中的研究进展张晶;张淑兰【摘要】Phosphoglycerate dehydrogenase (3-PGDH or PHGDH) is the key enzyme of de novo serine biosynthesis. The PHGDH gene located in human chromosomes 1p12, which is highly expressed in normal tissue, especially for the nervous system function. Recently, it was shown that a part of intermediate metabolites of glycolysis flew to serine biosynthesis. It was found PHGDH gene amplification and over expression in some tumor, PHGDH regulated tumor cell proliferation, apoptosis and invasion. Cell metabolism of tumor is benefit to tumor oncogenesis and progression.The study found that silence PHGDH can inhibit tumor cell proliferation, invasion and increase cell apoptosis. And PHGDH may be a potential treatment target. In this paper, we will review briefly the studies on PHGDH, including structural features, biological function and the genesis and development effect in some tumor.%3-磷酸甘油酸脱氢酶（3-PGDH或PHGDH）是丝氨酸合成途径的关键酶。

Keap1

非小细胞肺癌(non-small cell lung cancer，NSCLC)发病率占据肺癌的75%~80%。

肿瘤细胞进展快且易扩散转移，临床常采用手术、放化疗等进行治疗，但5年生存率低于60%[1-2]。

氧化应激是由活性氧(ROS)生成量增加所致，ROS积累可诱导肺癌细胞凋亡，清除ROS 可阻止癌细胞凋亡，即肺癌细胞存活依赖于癌细胞自身抗氧化能力[3]。

Kelch样环氧氯丙烷相关蛋白-1 (kelch-like epichlorohydrin-associated protein-1，Keap1)/核因子E2相关因子2(nuclear factor E2related factor 2，Nrf2)信号通路在癌症中发挥重要调控作用，氧化应激可激活Keap1，促使Keap1-Nrf2复合物裂解，Nrf2转移至细胞核内，可激活下游靶基因表达，参与肺癌发生发展过程[4]。

Nrf2可维持氧化还原稳态，ROS侵袭细胞时，Nrf2可进入细胞核，结合抗氧化反应元件(ARE)转录编码各种抗氧化蛋白、代谢酶基因，抑制氧化应激反应[5-6]。

目前氧化应激、Keap1/Nrf2信号通路在NSCLC发生过程中的机制尚未明确。

基于此，本研究尝试分析Keap1/Nrf2信号通路与临床病理参数、氧化应激指标的相关性，探讨其在NSCLC氧化应激机制中的作用，为临床研制新药提供参考依据。

1资料与方法1.1一般资料选取2017年4月至2020年4月郑州市第三人民医院收治的100例NSCLC患者为研究对象。

纳入标准：符合NSCLC诊断标准[7]；术前未接受放化疗、免疫治疗者；预计生存期≥6个月；符合手术适应证、禁忌证；Karnofsky功能状态评分≥70分；签署知情同意书。

排除标准：合并凝血功能障碍、肝肾功能障碍、其他恶性肿瘤者；伴有急/慢性感染者；伴有精神疾病者；既往腹部相关外科手术史者。

所有患者均行肺癌根治性切除术，术中收集癌组织、癌旁组织(距离癌组织5cm范围内正常组织)，其中男性63例，女性37例；年龄46~67岁，平均(56.32±3.16)岁；体质量指数(BMI)17~30kg/m2，平均(23.16±2.03)kg/m2；病理类型：鳞癌58例、腺癌42例；病理分级[8]：Ⅰ~Ⅱ级51例、Ⅲ级49例；T分期[9]：T1~T253例、T3~T447例；N分期：N055例、N1~N245例。

消化道多巴胺及其代谢酶的功能研究

消化道多巴胺及其代谢酶的功能研究摘要：多巴胺(dopamine, DA)是一种广泛存在于中枢神经系统和外周组织的儿茶酚胺类神经递质，其功能越来越受到学者们的关注，尤其是近年发现DA可以调节免疫系统功能，DA与肠黏膜炎症相关疾病联系的研究成为热点。

消化道是外周DA的重要来源，DA 不仅产生于肠神经系统和消化道上皮等部位，而且还大量产自于肠道微生物。

机体组织中DA的含量变化除了受其合成酶影响外，还受到两个重要的代谢酶单胺氧化酶(monoamine oxidase,MAO)和儿茶酚-O-甲基转移酶(catechol-O-methyltransferase,COMT)的调控。

本文主要对消化道DA的来源和功能、DA代谢酶的分布和功能进行综述。

关键词：消化道; 多巴胺; 单胺氧化酶; 儿茶酚氧位甲基转移酶;Abstract：Dopamine(DA), as a catecholamine neurotransmitter widely distributed in the central nervous system and the peripheral tissues, has attracted a lot of attention. Especially in recent years, DA has been found to regulate the function of the immune system, and the involvement of DA in the intestinal mucosal inflammation-related diseases has become a hot research topic. The digestive tract is an important source of peripheral DA, and DA is not only produced in the enteric nervous system and gastrointestinal epithelium, but also produced by intestinal microorganisms. In addition to the synthetases of DA, the DA contents in body tissues are also affected by the two kinds of metabolic enzymes, monoamine oxidase(MAO) and catechol-O-methyltransferase(COMT). This article reviewed the sources, metabolism and functions of DA in digestive tract, especially focusing on the distribution and function of MAO and COMT, the enzymes degrading DA.Keyword：gastrointestinal tract; dopamine; monoamine oxidase; catechol-O-methyltransferase;多巴胺(dopamine,DA)是一种重要的单胺类神经递质，不仅在中枢神经系统发挥调控运动、认知、情绪、记忆和奖赏等作用，在外周组织，尤其胃肠道中也发挥重要作用，包括保护胃肠黏膜，调节黏膜离子分泌，调节胃肠动力等。

医学术语谷氨酸脱氢酶英文

医学术语谷氨酸脱氢酶英文Title: Glutamate Dehydrogenase: A Key Enzyme in Amino Acid Metabolism.Glutamate Dehydrogenase (GDH) is an enzyme that plays a crucial role in amino acid metabolism. It catalyzes the reversible conversion of glutamate to alpha-ketoglutarate, a process that involves the oxidation of glutamate and the reduction of NAD+ to NADH+H+. This enzyme is found in various tissues of the body, including the liver, kidney, and brain, and it plays a vital role in maintaining the balance of amino acids in the body.The structure of GDH is complex, with multiple subunits that come together to form the active enzyme. The enzyme requires NAD+ or NADP+ as a cofactor for its activity, and it can exist in either the mitochondrial or cytosolic form, depending on the tissue and species.The function of GDH is diverse and depends on itssubcellular location. In the mitochondria, GDH plays a role in the Krebs cycle, where it converts glutamate to alpha-ketoglutarate, generating NADH+H+ in the process. This NADH+H+ can then be used by the electron transport chain to generate ATP, the cell's main energy currency. In the cytosol, GDH plays a role in amino acid catabolism, whereit converts glutamate to alpha-ketoglutarate, generating NADH+H+ in the process. This NADH+H+ can then be used by other enzymes in the cytosol for various biosynthetic reactions.The regulation of GDH activity is complex and involves multiple mechanisms. One of the main regulators of GDH activity is the availability of its substrates and cofactors. For example, an increase in the concentration of glutamate or NAD+ can stimulate GDH activity, while a decrease in these substrates can inhibit the enzyme. Additionally, GDH activity can be regulated by allosteric effectors, which bind to the enzyme and modify its activity. Some allosteric effectors, such as ADP and ATP, can inhibit GDH activity, while others, such as GTP, can stimulate the enzyme.The role of GDH in disease is also well-documented. Alterations in GDH activity have been observed in various diseases, including liver disease, kidney disease, and neurological disorders. For example, in liver disease, a decrease in GDH activity can lead to a decrease in the conversion of glutamate to alpha-ketoglutarate, resultingin an accumulation of glutamate in the blood. This accumulation can lead to neurological symptoms such as seizures and coma. Similarly, in kidney disease, a decrease in GDH activity can lead to an accumulation of glutamate in the urine, which can contribute to the development ofkidney stones.In conclusion, Glutamate Dehydrogenase is a key enzymein amino acid metabolism that plays a crucial role in maintaining the balance of amino acids in the body. Its function is diverse and depends on its subcellular location, and its activity is regulated by multiple mechanisms. Alterations in GDH activity have been observed in various diseases, indicating its importance in maintaining thehealth of the body. Future research on GDH may lead to abetter understanding of its role in disease and the development of new therapeutic strategies.。

尿中3-甲基腺嘌呤的毛细管气相色谱-质谱分析

尿中3-甲基腺嘌呤的毛细管气相色谱-质谱分析
许后效;金秀兰
【期刊名称】《环境化学》
【年(卷),期】1995(14)3
【摘要】生物标志(Biomarker)是目前环境化学致突变剂研究的重要问题之一,我们研究了尿中3-甲基腺嘌呤作为人类暴露于环境中甲基化致突变剂的生物标志的可能性,本文首先概述了尿中3-甲基腺嘌呤的生成机理,研究了用气相色谱质谱联用仪(GC/MS)测定尿中3-甲基腺嘌呤的方法以及尿样的纯化分离程序,还测定了正常健康人和吸烟者尿样中3-甲基腺嘌呤的含量水平.结果表明,吸烟者尿中的3-甲基腺嘌呤的含量水平高于正常健康人的两倍左右.文章最后讨论了关于3-甲基腺嘌呤作为甲基致突变剂暴露的生物标志的可能性及其应用前景.
【总页数】4页(P211-214)
【关键词】甲基化致突变剂;甲基腺嘌呤;生物标志;气相色谱
【作者】许后效;金秀兰
【作者单位】中国科学院生态环境研究中心
【正文语种】中文
【中图分类】X132
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2.气相色谱-质谱联用技术在3-羟基-3-甲基戊二酸尿症诊断中的应用 [J], 江敏妍;刘丽;李秀珍;程静;蔡燕娜;彭敏芝
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