Development of a PC Based Generic Radar Display Simulator
WHO社区康复指南导论篇
华中科技大学 同济医院 世界卫生组织康复培训与研究合作中心(武汉) 中山大学 附属第一医院 世界卫生组织康复合作中心(广州) 香港复康会 世界卫生组织复康协作中心(香港)
2011-9
Ⅲ
社区康复指南 内容
1. 导论篇 2. 健康篇 3. 教育篇 4. 谋生篇 5. 社会篇 6. 赋能篇 7. 增补篇
中文版:由 华中科技大学 同济医院 世界卫生组织康复培训与研究合作中心(武汉)、 中山大学 附属第一医院 世界卫生组织康复合作中心(广州)与 香港复康会 世界卫生组织复康协作中心(香港) 联合组织译编
印刷: 中国 2011
朝向 包融性社区发展
Ⅰ
序言
《社区康复指南》出版了,对于残疾人工作者这是一本重要的手册。当我们 面对需要各种帮助的残疾人,或许会有困惑和疑问:怎样帮助他们获得更好的康 复和服务,怎样帮助他们融入社会生活。我想,建立社区服务是一个最好的途径。 近些年,我们看到一些国家和地区越来越重视残疾人的公共服务、在保障残疾人 权益方面有了新的进步。但是,什么才是最好的服务模式呢?这是一个值得探索 和探讨的问题。《社区康复指南》所记录的正是 30 多年来有关国际组织,包括 中国在内的 90 多个国家探索社区服务的模式和经验。
中国残疾人联合会主席 张海迪
2011 年 9 月 7 日
Ⅱ
前言
根据 2011.6 月世界卫生组织正式公布的《世界残疾报告》,世界人口中至少 有 15%的人带有残疾,他们对康复的需求殷切。机构康复以及延伸服务,远远不 能满足其要求。世界卫生组织 1978 年国际初级卫生保健大会及阿拉木图宣言之 后,提倡社区康复(Community-based rehabilitation, CBR),作为一种策略,在发 展中国家促使广大残疾人得到康复服务,在过去 30 年,其范围已得到相当大的 扩展。1994 年国际劳工组织、联合国教科文组织以及世界卫生组织共同制定了 《社区康复联合意见书》。2003 年,在赫尔辛基召开的国际社区康复回顾与咨询 大会做出了很多重要建议。随后,国际劳工组织、联合国教科文组织以及世界卫 生组织的共同修订了《2004 社区康复联合意见书》。在 2004 年 11 月,该三国际 组织邀请了 65 位社区康复及残疾、发育方面的专家开始制定《社区康复指南》。 广泛收集资料,有 150 多位作者为该指南撰稿。草稿经过代表世界卫生组织各地 区的 29 个国家的广泛确定。总共有 300 多位完成社区康复的项目人对草稿提供 了反馈。指南于 2010 年 5 月 19 日被批准出版。预计指南的有效期可保持到 2020 年。指南受到《残疾人权利公约》的深刻影响,是贯彻执行公约的具体体现。
英文作文伟大发明家
英文作文伟大发明家Title: The Greatest Inventors: Shaping the World Through Ingenuity.In the annals of history, the names of great inventors stand tall, their contributions etching indelible marks on the tapestry of human progress. These are the men and women who, through their brilliant minds and relentless dedication, have transformed our world, making it safer, more convenient, and incredibly interconnected.Thomas Edison, a paragon of perseverance, is often remembered for his groundbreaking invention of the light bulb. However, his legacy extends far beyond this single accomplishment. Edison's laboratories were hotbeds of innovation, giving birth to the phonograph, the motion picture camera, and countless other groundbreaking technologies. His perseverance in the face of repeated failures, as he famously said, "I have not failed. I'vejust found 10,000 ways that won't work," is an inspirationto us all.Albert Einstein's contributions to science are so profound that they have altered our fundamental understanding of the universe. His Theory of Relativity revolutionized physics, introducing the concepts of space-time curvature and the equivalence of mass and energy, expressed in the famous equation E=mc^2. Einstein's legacy is not just in his groundbreaking theories but also in his unwavering commitment to intellectual curiosity and the search for truth.Another towering figure in the history of invention is James Watson and Francis Crick, who, along with Rosalind Franklin, unlocked the secret of life itself with their discovery of the structure of DNA. This molecular double helix underpins all known life forms, encoding the genetic information that determines our very existence. Watson and Crick's groundbreaking work has had profound implications for medicine, biotechnology, and our understanding of ourselves.In the realm of computing, few names are as synonymous with innovation as Steve Jobs. As the cofounder of Apple Inc., Jobs revolutionized personal computing with products like the Macintosh, introducing the world to graphical user interfaces, intuitive mouse controls, and the concept of "personal" computing. His visionary leadership and design sensibilities have left an indelible mark on the technology industry, influencing not just computers but also smartphones, tablets, and other devices that have become ubiquitous in modern life.These are just a few examples of the great inventors who have shaped our world. Each of them embodies the spirit of invention: a relentless curiosity, an unwavering commitment to solving problems, and a refusal to be satisfied with the status quo. Their legacies are not just in the specific inventions they created but also in the way they transformed our worldviews and expectations.The journey of invention is often fraught with challenges and failures, but it is also filled with the promise of progress and transformation. As we look to thefuture, it is exciting to imagine what new technologies and ideas await us. Will we see the development of sustainable energy solutions that power our world cleanly and efficiently? Will advances in biotechnology lead to new cures for diseases that have plagued humanity for centuries? Will artificial intelligence and machine learning lead to a new era of human-computer symbiosis?The answers to these questions remain to be seen, but one thing is certain: the spirit of invention will continue to drive human progress. Just as Edison, Einstein, Watson, Crick, and Jobs have done before us, future generations of inventors will continue to push the boundaries of what is possible, shaping our world in ways we can only imagine. In their footsteps, we too can contribute to this legacy of progress and transformation, making our own marks on the tapestry of human history.。
微软无线光学桌面4000系列说明书
Version InformationProduct Name Microsoft® Wireless Optical Desktop 4000Product Version Microsoft Wireless Optical Desktop 4000 v1.0Keyboard Version Microsoft Wireless Comfort Keyboard 1.0aMouse Version Microsoft Wireless Optical Mouse 2.0aReceiver Version Microsoft Wireless Optical Desktop Receiver 3.0aProduct DimensionsKeyboard Length19.2 inches (487 millimeters)Keyboard Width9.69 inches (246 millimeters) includes palm restKeyboard Depth/Height 2.24 inches (57 millimeters) with tilt legs extendedKeyboard Weight34.3 ounces (971 grams) includes 2 AA alkaline batteries; typical battery weight may varyMouse Length 4.82 inches (123 millimeters)Mouse Width 2.89 inches (73.5 millimeters)Mouse Depth/Height 1.69 inches (43.0 millimeters)Mouse Weight 4.81 ounces (136 grams) includes 2 AA alkaline batteries; typical battery weight may varyReceiver Length 4.72 inches (120 millimeters)Receiver Width 3.15 inches (80.0 millimeters)Receiver Depth/Height 1.13 inches (28.7 millimeters)Receiver Weight 5.92 ounces (168 grams)Receiver Cable Length60 inches + 6/-0 inches (1524 millimeters +152/-0 millimeters)Compatibility and LocalizationInterface USB and PS/2 CompatibleOperating Systems• Microsoft Windows® XP Professional/Home Edition/Media Center Edition/Tablet PC Edition/Windows 2000• Macintosh Mac OS X versions 10.1.x-10.3.x (excludes Mac OS X version 10.0)Top-line System Requirements For a Microsoft Windows-based PC:• Microsoft Windows XP Professional/Home Edition/Media Center Edition/Tablet PC Edition/Windows 2000• Pentium 233 MHz or higher processor• 128 MB of RAM• 60 MB of available hard disk space (install 100 MB free)• USB port or PS/2 port• CD driveFor a Macintosh PC:• Macintosh Mac OS X versions 10.1.x-10.3.x (excludes Mac OS X version 10.0)• 30 MB of available hard disk space• USB port• CD driveCompatibility Logos• Designed for Microsoft Windows XP and Windows 2000• Mac logo• Certified USB logoSoftware Version Microsoft IntelliPoint software, version 5.3 or higher, is required in order to enjoy full product functionality (including Tilt Wheel Technology, Smart Receiver Technology, and button programmability features). Microsoft IntelliType Pro, version 5.3 orhigher, is required in order to enjoy full product functionality (including all Zoom Slider, Hot Key, and Media Key features).Internet Hot Keys are supported for use with:• Windows-based PC: Microsoft Internet Explorer version 5.0 or later; MSN® Explorer versions 7.0 and 8.0; NetscapeNavigator versions 6.1, 6.2, and 7.0; and AOL versions 7.0, 8.0, and 9.0• Macintosh PC: Microsoft Internet Explorer for the Macintosh version 5.0 or later and Netscape Navigator version 6.1 or 7.0Multimedia Hot Keys are supported for use with:• Windows-based PC: Versions of Microsoft CD Player, Microsoft Deluxe CD, Microsoft Windows Media® Player, and certainversions of other popular media players and browsers• Macintosh: iTunesSleep Hot Key functionality is available if your system supports power management and has it enabled, and if all peripheralsare compatible with power management.Software Localization For a Microsoft Windows-based PC:Microsoft IntelliPoint and IntelliType Pro software may be installed in Simplified Chinese, Traditional Chinese, English,French, German, Italian, Japanese, Korean, Brazilian Portuguese, Iberian Portuguese, or Spanish. If available, standardsetup will install the software in the default OS language. Otherwise, the English language version will be installed.For a Macintosh PC:Microsoft IntelliPoint for Mac and IntelliType Pro for Mac will install in English, French, German, Italian, Japanese, BrazilianPortuguese, and Spanish. User settings for OS language preference determine which localized version of the software isdisplayed. In the event that OS language preferences are not supported, the English language versions of IntelliPoint for Macand IntelliType Pro for Mac will be displayed.Keyboard Localization Available language sets will vary by product and sales channel.104 key configuration: Chinese (Traditional), English (North America), Greek, International English (EURO), andInternational English (ROW)105 key configuration: Danish, English (UK), Finnish/Swedish, French (Belgium), French (Canada), French (France),German (Germany), German (Switzerland/Belgium), Hebrew, Italian, Norwegian, Portuguese (Portugal), Spanish (EURO),and Spanish (Latin America)106 key configuration: Korean109 key configuration: Chinese (Simplified)and JapaneseTracking TechnologyMouse Tracking System Microsoft-proprietary optical technologyImaging Rate Dynamically adaptable to 6000 frames per secondX-Y Resolution400 points per inch (15.75 points per millimeter)Tracking Speed Up to 36 inches (914 millimeters) per secondWireless TechnologyWireless Platform27 MHz Radio Frequency (RF)Wireless Channels• Keyboard: 27.095 MHz channel 0, 27.195 MHz channel 1• Mouse: 27.145 MHz Mouse channel 1Wireless IDs• Keyboard: Over 65,000 random identification codes• Mouse: Over 65,000 random identification codesWireless Range 6 feet (1.83 meters) typical. Note: RF range is affected by many factors, such as nearby metallic objects and relativepositioning of the keyboard, mouse, and receiver.Product Feature PerformanceQWERTY Key Life1,000,000 actuations per keyZoom Slider Life250,000 actuations in either directionHot Key Features Web/Home, Calendar, Mail, Messenger, My Documents, Show My Favorites, My Favorites 1, My Favorites 2, My Favorites 3, My Favorites 4, My Favorites 5, Calculator, Log Off, and SleepHot Key Life500,000 actuations per keyMedia Key Features Mute, Volume -, Volume +, Play/Pause, Stop (Media), Previous Track, and Next TrackMedia Key Life500,000 actuations per keyEnhanced Function Key Features Help, Undo, Redo, New, Open, Close, Reply, Forward, Send, Spell, Save, and PrintTyping Speed1000 characters per minuteMouse Button Features 3 buttons including scroll wheel buttonRight & Left Button Life1,000,000 actuations at no more than 4 actuations per secondWheel Button Life150,000 actuations at no more than 4 actuations per secondMouse Scrolling Features Tilt wheel enables vertical and horizontal scrollingWheel Vertical Scrolling Life• 100,000 revolutions (away from user)• 100,000 revolutions (towards user)Wheel Horizontal Scrolling Life350,000 actuations per side at no more than 4 actuations per secondStorage Temperature & Humidity-40 °F (-40 °C) to 140 °F (60 °C) at < 5% to 65% relative humidity (non-condensing)Operating Temperature & Humidity14 °F (-10 °C) to 104 °F (40 °C) at <5% to 80% relative humidity (non-condensing)Power RequirementsBattery Type and Quantity• Keyboard: 2 AA alkaline batteries (included)• Mouse: 2 AA alkaline batteries (included)Battery Life• Keyboard: 6 months typical• Mouse: 6 months typicalCertification InformationCountry of Manufacture Keyboard and Receiver: Thailand and Mouse: People's Republic of China (PRC)ISO 9002 Qualified Manufacturer YesFCC ID This device complies with part 15 of the FCC Rules and Industry Canada RSS-210. Operation is subject to the following two conditions: (1) This device may not cause harmful interference, and (2) this device must accept any interference received,including interference that may cause undesired operation. Tested to comply with FCC standards. For home and office use.Model numbers: 1027, Wireless Optical Comfort Keyboard 1.0; 1008, Wireless Optical Mouse 2.0; and 1029, WirelessOptical Desktop Receiver 3.0. FCC IDs: C3K1027 and C3K1008.Agency and Regulatory Approvals• FCC Declaration of Conformity (USA)• UL and cUL Listed Accessory (USA and Canada)• RSS-210 and ICES-003 data on file (Canada)• TUV-T Certificate (European Union)• R&TTE Declaration of Conformity, Safety and EMC (European Union)• GOST Certificate (Russia)• VCCI Certificate (Japan)• ACA/MED Declaration of Conformity (Australia and New Zealand)• BSMI and DGT Certificates (Taiwan)• MIC Certificate (Korea)• CMII Certificate (China)• NOM Certificates (Mexico)• SABS Certificate (South Africa)• CB Scheme Certificate (International)• WHQL (International) ID: 890572Results stated herein are based on internal Microsoft testing. Individual results and performance may vary. Any device images shown are not actual size. This document is provided for informational purposes only and is subject to change without notice. Microsoft makes no warranty, express or implied, with this document or the information contained herein. Review any public use or publications of any data herein with your local legal counsel.©2006 Microsoft Corporation. All rights reserved. Microsoft, the IntelliEye logo, IntelliMouse, MSN, the Laser Technology logo, the Optical Technology logo, Natural, Windows, and Windows NT are either registered trademarks or trademarks of Microsoft Corporation in the U.S. and/or other countries. Mac and the Mac logo are trademarks of Apple Computer, Inc., registered in the U.S. and/or other countries. The names of actual companies and products mentioned herein may be trademarks of their respective owners.。
戴尔 PowerSwitch S3048-ON 1000BASE-T 交换机说明书
High density 1000BASE-T switchThe Dell PowerSwitch S3048-ON 1000BASE-T top-of-rack (ToR) switch is the industry’s first 1GbE enterprise switching platform to deliver both an industry hardened OS and support for open networking, providing freedom to run third-party operating systems (OS).This open networking platform is built for high-performance, software-defined data centers and provides the features to run traditional workloadsand the flexibility to deploy new workloads such as Hadoop, SDS and Big Data. The S3048-ON offersthe flexibility to run OS options optimized for diverse deployment needs on a common hardware platform and architecture.The S3048-ON features a non-blocking switching architecture coupled with OS9.X software, delivering line-rate L2/L3 features for maximized network performance. The S3048-ON design provides (48) 1000BASE-T ports that support 10MbE/100MbE/1GbE and four 10GbE SFP+ uplinks. Each 10GbE interface can be used as uplinks to the network spine/core,as stack ports to connect up to six units in a stacked configuration, or a combination of both, dependingon network architecture and uplink/stack bandwidth requirements.The S3048-ON incorporates multiple architectural features that optimize data center network flexibility, efficiency and availability including:• I/O panel to PSU airflow or PSU to I/O panel airflow for hot/cold aisle environments• Redundant, hot-swappable power supplies and fans with color coded touch points for ease of identification/removal• Dell ReadyRails for efficient installation of the switch into data center cabinetsThe S3048-ON also supports Dell Technologies’ Embedded Open Automation Framework, which provides advanced network automation and virtualization capabilities for virtual data center environments. Embedded Open Automation Framework is a suite of network management apps that can be used together or independently to provide a network that is flexible, available and manageable while helping to reduce operational expenses. Key applications• High-density 1000BASE-T ToR server aggregation in high-performance data centers environments• Active Fabric™ designs with the S- or Z-Series core switch to create a two tier, 1/10/40GbE data centernetwork architecture• Enterprise, Web 2.0 and cloud service providers’ data center networks for ToR applications• High-performance SDN/OpenFlow 1.3 enabled with ability to inter-operate with industry standard Open-Flow controllersKey features• Scalable L2 and L3 Ethernet switching with QoS anda full complement of standards-based IPv4 and IPv6features, including OSPF, BGP and PBR (Policy Based Routing) support• Four SFP+ 10GbE ports for maximum flexibility and investment protection• I/O panel to PSU airflow or PSU to I/O panel airflow • Redundant, hot-swappable power supplies and fans • Supports ONIE for zero-touch installation of alternate network operating systems• Open Networking offers choice of OS, such as Dell SmartFabric OS10 and Dell OS9, for inherent stability and feature richness, or the flexibility of a third-partyOS• VRF-lite enables sharing of networking infrastructure and provides L3 traffic isolation acrosstenants (including support for multicast and IPv6routing)• Enhanced automation capabilities (puppet agent, REST API extensions)• Supports jumbo frames for high-end performance in virtualized environments and IP storage/server communi-cation• Increase VM Mobility region by stretching L2 VLAN within or across two DCs with unique VLT capabilities like Routed VLT, VLT Proxy Gateway• User port stacking support for up to six units managed as one logical device• Embedded Open Automation Framework adds VM awareness automated configuration and provisioningDELL POWERSWITCH S3048-ON 1GbE top-of-rack open networking switchNote: In-field change of airflow direction not supported. *Ordered separatelyPhysical48 line-rate 1000BASE-T ports4 line-rate 10GbE SFP+ ports1 RJ45 console/management port with RS232signalingSize: 1 RU, 1.71”h x 17.09” w x 12.6” d (4.4 h x43.4 w x 32.0 cm d)Weight: 12.8 lbs (5.84 kg) with 1 power supply,14.8 lbs (6.74kg) with 2 power suppliesISO 7779 A-weighted sound pressure level: <36 dBA at 78.8°F (26°C)Power supply: 90–264 VAC 50/60 Hz1) AC forward airflow2) AC reverse airflowMax. thermal output: 290 BTU/hMax. current draw per system:<1A at 100/120V VAC <0.5A at 200/240VAC Max. power consumption: 87WTyp. power consumption: 65 WattsMax. operating specifications:Operating temperature: 32° to 113°F (0° to 45°C)Operating humidity: 5 to 85% (RH), non-condensingOperating altitude: 0ft to 10,000ft above sea levelMax. non-operating specifications:Storage temperature: –40° to 158°F (–40° to70°C)Storage humidity: 5 to 95% (RH), non-condensingRedundancyHot swappable redundant power suppliesHot swappable redundant fansUser port stacking up to 6 unitsPerformanceMAC addresses: up to 80kIPv4 routes: 16KIPv6 routes: 8K (shared CAM space with IPv4) Switch fabric capacity: 260Gbps (full-duplex) 130 Gbps (half-duplex)Forwarding capacity: 131 MppsLink aggregation: 16 links per group, 128 groups per stackQueues per port: 8 queuesLayer 2 VLANs: 4KMSTP : 64 instancesVRF-lite: 64 instancesLine-rate Layer 2 switching: all protocols, including IPv4 and IPv6Line-rate Layer 3 routing: IPv4 and IPv6IPv4 host table size up to 40k maxIPv6 host table size 8KIPv4 Multicast table size 8KLAG load balancing: based on Layer 2, IPv4 or IPv6 headersLatency 3.7 μsec for 1000BASE-T, ~1.8 μsec for SFP+Packet buffer memory: 4MBCPU memory: 2GBIEEE compliance802.1AB LLDP802.1D Bridging, STP802.1p L2 Prioritization802.1Q VLAN T agging802.1s MSTP802.1w RSTP802.1X Network Access Control802.3ab Gigabit Ethernet (1000BASE-T)802.3ac Frame Extensions for VLAN T agging 802.3ad Link Aggregation with LACP802.3ae 10 Gigabit Ethernet (10GBASE-X) on optical ports 802.3az Energy Efficient Ethernet (EEE)802.3u Fast Ethernet (100BASE-TX)on mgmt ports802.3x Flow Control802.3z Gigabit Ethernet(1000BASE-X)ANSI/TIA-1057 LLDP-MEDForce10 PVST+MTU 12,000 bytesRFC and I-D complianceGeneral Internet protocols768 UDP793 TCP854 Telnet959 FTPGeneral IPv4 protocols791 IPv4792 ICMP826 ARP1027 Proxy ARP1035 DNS (client)1042 Ethernet Transmission1305 NTPv31519 CIDR1542 BOOTP (relay)1812 Requirements for IPv4 Routers1918 Address Allocation for PrivateInternets2474 Diffserv Field in IPv4 and Ipv6Headers2596 Assured Forwarding PHB Group3164 BSD Syslog3195 Reliable Delivery for Syslog3246 Expedited Assured Forwarding4364 VRF-lite (IPv4 VRF with OSPF,BGP, IS-IS, and v4 multicast)5798 VRRPGeneral IPv6 protocols1981 Path MTU Discovery Features2460 Internet Protocol, Version 6(IPv6) Specification2464 Transmission of IPv6 Packetsover Ethernet Networks2711 IPv6 Router Alert Option4007 IPv6 Scoped AddressArchitecture4213 Basic Transition Mechanisms forIPv6 Hosts and Routers4291 IPv6 Addressing Architecture4443 ICMP for IPv64861 Neighbor Discovery for IPv64862 IPv6 Stateless AddressAutoconfiguration5095 Deprecation of Type 0 RoutingHeaders in IPv6IPv6 Management support (telnet,FTP, TACACS, RADIUS, SSH,NTP)VRF-Lite (IPv6 VRF with OSPFv3, BGPv6, andIS-IS)RIP1058 RIPv1 2453 RIPv2OSPF (v2/v3)1587 NSSA 4552 Authentication/2154 OSPF Digital Signatures2328 OSPFv2 OSPFv32370 Opaque LSA 5340 OSPF forIPv6IS-IS5301 Dynamic hostname exchangemechanism for IS-IS5302 Domain-wide prefix distributionwith two-level IS-IS5303 Three way handshake for IS-IS point-to-pointadjacencies5308 IS-IS for IPv6BGP1997 Communities2385 MD52545 BGP-4 Multiprotocol Extensions forIPv6 Inter-Domain Routing2439 Route Flap Damping2796 Route Reflection2842 Capabilities2858 Multiprotocol Extensions2918 Route Refresh3065 Confederations4360 Extended Communities4893 4-byte ASN5396 4-byte ASN representationsdraft-ietf-idr-bgp4-20 BGPv4draft-michaelson-4byte-as-representation-054-byte ASN Representation (partial)draft-ietf-idr-add-paths-04.txt ADD PATHMulticast1112 IGMPv12236 IGMPv23376 IGMPv3MSDPdraft-ietf-pim-sm-v2-new-05PIM-SMwNetwork management1155 SMIv11157 SNMPv11212 Concise MIB Definitions1215 SNMP Traps1493 Bridges MIB1850 OSPFv2 MIB1901 Community-Based SNMPv22011 IP MIB2096 IP Forwarding Table MIB2578 SMIv22579 Textual Conventions for SMIv22580 Conformance Statements for SMIv22618 RADIUS Authentication MIB2665 Ethernet-Like Interfaces MIB2674 Extended Bridge MIB2787 VRRP MIB2819 RMON MIB (groups 1, 2, 3, 9)2863 Interfaces MIB3273 RMON High Capacity MIB3410 SNMPv33411 SNMPv3 Management Framework3412 Message Processing and Dispatchingfor the Simple Network ManagementProtocol(SNMP)3413 SNMP Applications3414 User-based Security Model (USM) forSNMPv33415 VACM for SNMP3416 SNMPv23417 Transport mappings for SNMP3418 SNMP MIB3434 RMON High Capacity Alarm MIB3584 Coexistance between SNMP v1, v2andv34022 IP MIB4087 IP Tunnel MIB4113 UDP MIB4133Entity MIB4292 MIB for IP4293 MIB for IPv6 Textual Conventions 4502 RMONv2 (groups 1,2,3,9)5060 PIM MIBANSI/TIA-1057 LLDP-MED MIBDell_ITA.Rev_1_1 MIBdraft-grant-tacacs-02 TACACS+draft-ietf-idr-bgp4-mib-06 BGP MIBv1IEEE 802.1AB LLDP MIBIEEE 802.1AB LLDP DOT1 MIBIEEE 802.1AB LLDP DOT3 MIB sFlowv5 sFlowv5 MIB (version 1.3) FORCE10-BGP4-V2-MIB Force10 BGP MIB (draft-ietf-idr-bgp4-mibv2-05)FORCE10-IF-EXTENSION-MIBFORCE10-LINKAGG-MIBFORCE10-COPY-CONFIG-MIBFORCE10-PRODUCTS-MIBFORCE10-SS-CHASSIS-MIBFORCE10-SMIFORCE10-TC-MIBFORCE10-TRAP-ALARM-MIBFORCE10-FORWARDINGPLANE-STATS-MIB 3376 IGMPv3MSDPdraft-ietf-pim-sm-v2-new-05 PIM-SMw Regulatory complianceSafetyUL/CSA 60950-1, Second EditionEN 60950-1, Second EditionIEC 60950-1, Second Edition Including AllNational Deviations and Group DifferencesEN 60825-1 Safety of Laser Products Part 1:Equipment Classification Requirementsand User’s GuideEN 60825-2 Safety of Laser Products Part 2:Safety of Optical Fibre CommunicationSystemsFDA Regulation 21 CFR 1040.10 and 1040.11EmissionsAustralia/New Zealand: AS/NZS CISPR 22:2006, Class ACanada: ICES-003, Issue-4, Class AEurope: EN 55022: 2006+A1:2007 (CISPR 22: 2006),Class AJapan: VCCI V3/2009 Class AUSA: FCC CFR 47 Part 15, Subpart B:2011,Class AImmunityEN 300 386 V1.4.1:2008 EMC for NetworkEquipmentEN 55024: 1998 + A1: 2001 + A2: 2003EN 61000-3-2: Harmonic Current EmissionsEN 61000-3-3: Voltage Fluctuations and FlickerEN 61000-4-2: ESDEN 61000-4-3: Radiated ImmunityEN 61000-4-4: EFTEN 61000-4-5: SurgeEN 61000-4-6: Low FrequencyConducted ImmunityRoHSAll S Series components are EU RoHScompliant.CertificationsAvailable with US Trade Agreements Act (TAA)complianceUSGv6 Host and Router Certified on DellNetworking OS 9.7 and greaterIPv6 Ready for both Host and RouterUCR DoD APL (core and distribution ASLANswitch)Tested to meet or exceed Hi Pot and GroundContinuity testing per UL 60950-1Warranty1 year return to depotContact a Dell Technologies ExpertView more resourcesLearn more about Dell Networking solutions Join the conversation with@DellNetworkingIT Lifecycle Services for NetworkingExperts, insights and easeOur highly trained experts, with innovative tools and proven processes, help you transform your IT investments into strategic advantages.Plan & DesignLet us analyze your multivendor environment and deliver a comprehensive report and action plan to build upon the existing network and improve performance.Deploy & IntegrateGet new wired or wireless network technology installed and configured with ProDeploy. Reduce costs, save time, and get up and running fast.EducateEnsure your staff builds the right skills for long-term success. Get certified on Dell Networking technology and learn how to increase performance and optimize infrastructure.Manage & SupportGain access to technical experts and quickly resolve multivendor networking challenges with ProSupport. Spend less time resolving network issues and more time innovating.OptimizeMaximize performance for dynamic ITenvironments with Dell Optimize. Benefit from in-depth predictive analysis, remote monitoring and a dedicated systems analyst for your network.RetireWe can help you resell or retire excess hardware while meeting local regulatory guidelines and acting in an environmentally responsible way.Learn more at /Services。
IEC-61854架空线.隔离层的要求和检验
NORMEINTERNATIONALECEI IEC INTERNATIONALSTANDARD 61854Première éditionFirst edition1998-09Lignes aériennes –Exigences et essais applicables aux entretoisesOverhead lines –Requirements and tests for spacersCommission Electrotechnique InternationaleInternational Electrotechnical Commission Pour prix, voir catalogue en vigueurFor price, see current catalogue© IEC 1998 Droits de reproduction réservés Copyright - all rights reservedAucune partie de cette publication ne peut être reproduite niutilisée sous quelque forme que ce soit et par aucunprocédé, électronique ou mécanique, y compris la photo-copie et les microfilms, sans l'accord écrit de l'éditeur.No part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical,including photocopying and microfilm, without permission in writing from the publisher.International Electrotechnical Commission 3, rue de Varembé Geneva, SwitzerlandTelefax: +41 22 919 0300e-mail: inmail@iec.ch IEC web site http: //www.iec.chCODE PRIX PRICE CODE X– 2 –61854 © CEI:1998SOMMAIREPages AVANT-PROPOS (6)Articles1Domaine d'application (8)2Références normatives (8)3Définitions (12)4Exigences générales (12)4.1Conception (12)4.2Matériaux (14)4.2.1Généralités (14)4.2.2Matériaux non métalliques (14)4.3Masse, dimensions et tolérances (14)4.4Protection contre la corrosion (14)4.5Aspect et finition de fabrication (14)4.6Marquage (14)4.7Consignes d'installation (14)5Assurance de la qualité (16)6Classification des essais (16)6.1Essais de type (16)6.1.1Généralités (16)6.1.2Application (16)6.2Essais sur échantillon (16)6.2.1Généralités (16)6.2.2Application (16)6.2.3Echantillonnage et critères de réception (18)6.3Essais individuels de série (18)6.3.1Généralités (18)6.3.2Application et critères de réception (18)6.4Tableau des essais à effectuer (18)7Méthodes d'essai (22)7.1Contrôle visuel (22)7.2Vérification des dimensions, des matériaux et de la masse (22)7.3Essai de protection contre la corrosion (22)7.3.1Composants revêtus par galvanisation à chaud (autres queles fils d'acier galvanisés toronnés) (22)7.3.2Produits en fer protégés contre la corrosion par des méthodes autresque la galvanisation à chaud (24)7.3.3Fils d'acier galvanisé toronnés (24)7.3.4Corrosion causée par des composants non métalliques (24)7.4Essais non destructifs (24)61854 © IEC:1998– 3 –CONTENTSPage FOREWORD (7)Clause1Scope (9)2Normative references (9)3Definitions (13)4General requirements (13)4.1Design (13)4.2Materials (15)4.2.1General (15)4.2.2Non-metallic materials (15)4.3Mass, dimensions and tolerances (15)4.4Protection against corrosion (15)4.5Manufacturing appearance and finish (15)4.6Marking (15)4.7Installation instructions (15)5Quality assurance (17)6Classification of tests (17)6.1Type tests (17)6.1.1General (17)6.1.2Application (17)6.2Sample tests (17)6.2.1General (17)6.2.2Application (17)6.2.3Sampling and acceptance criteria (19)6.3Routine tests (19)6.3.1General (19)6.3.2Application and acceptance criteria (19)6.4Table of tests to be applied (19)7Test methods (23)7.1Visual examination (23)7.2Verification of dimensions, materials and mass (23)7.3Corrosion protection test (23)7.3.1Hot dip galvanized components (other than stranded galvanizedsteel wires) (23)7.3.2Ferrous components protected from corrosion by methods other thanhot dip galvanizing (25)7.3.3Stranded galvanized steel wires (25)7.3.4Corrosion caused by non-metallic components (25)7.4Non-destructive tests (25)– 4 –61854 © CEI:1998 Articles Pages7.5Essais mécaniques (26)7.5.1Essais de glissement des pinces (26)7.5.1.1Essai de glissement longitudinal (26)7.5.1.2Essai de glissement en torsion (28)7.5.2Essai de boulon fusible (28)7.5.3Essai de serrage des boulons de pince (30)7.5.4Essais de courant de court-circuit simulé et essais de compressionet de traction (30)7.5.4.1Essai de courant de court-circuit simulé (30)7.5.4.2Essai de compression et de traction (32)7.5.5Caractérisation des propriétés élastiques et d'amortissement (32)7.5.6Essais de flexibilité (38)7.5.7Essais de fatigue (38)7.5.7.1Généralités (38)7.5.7.2Oscillation de sous-portée (40)7.5.7.3Vibrations éoliennes (40)7.6Essais de caractérisation des élastomères (42)7.6.1Généralités (42)7.6.2Essais (42)7.6.3Essai de résistance à l'ozone (46)7.7Essais électriques (46)7.7.1Essais d'effet couronne et de tension de perturbations radioélectriques..467.7.2Essai de résistance électrique (46)7.8Vérification du comportement vibratoire du système faisceau/entretoise (48)Annexe A (normative) Informations techniques minimales à convenirentre acheteur et fournisseur (64)Annexe B (informative) Forces de compression dans l'essai de courantde court-circuit simulé (66)Annexe C (informative) Caractérisation des propriétés élastiques et d'amortissementMéthode de détermination de la rigidité et de l'amortissement (70)Annexe D (informative) Contrôle du comportement vibratoire du systèmefaisceau/entretoise (74)Bibliographie (80)Figures (50)Tableau 1 – Essais sur les entretoises (20)Tableau 2 – Essais sur les élastomères (44)61854 © IEC:1998– 5 –Clause Page7.5Mechanical tests (27)7.5.1Clamp slip tests (27)7.5.1.1Longitudinal slip test (27)7.5.1.2Torsional slip test (29)7.5.2Breakaway bolt test (29)7.5.3Clamp bolt tightening test (31)7.5.4Simulated short-circuit current test and compression and tension tests (31)7.5.4.1Simulated short-circuit current test (31)7.5.4.2Compression and tension test (33)7.5.5Characterisation of the elastic and damping properties (33)7.5.6Flexibility tests (39)7.5.7Fatigue tests (39)7.5.7.1General (39)7.5.7.2Subspan oscillation (41)7.5.7.3Aeolian vibration (41)7.6Tests to characterise elastomers (43)7.6.1General (43)7.6.2Tests (43)7.6.3Ozone resistance test (47)7.7Electrical tests (47)7.7.1Corona and radio interference voltage (RIV) tests (47)7.7.2Electrical resistance test (47)7.8Verification of vibration behaviour of the bundle-spacer system (49)Annex A (normative) Minimum technical details to be agreed betweenpurchaser and supplier (65)Annex B (informative) Compressive forces in the simulated short-circuit current test (67)Annex C (informative) Characterisation of the elastic and damping propertiesStiffness-Damping Method (71)Annex D (informative) Verification of vibration behaviour of the bundle/spacer system (75)Bibliography (81)Figures (51)Table 1 – Tests on spacers (21)Table 2 – Tests on elastomers (45)– 6 –61854 © CEI:1998 COMMISSION ÉLECTROTECHNIQUE INTERNATIONALE––––––––––LIGNES AÉRIENNES –EXIGENCES ET ESSAIS APPLICABLES AUX ENTRETOISESAVANT-PROPOS1)La CEI (Commission Electrotechnique Internationale) est une organisation mondiale de normalisation composéede l'ensemble des comités électrotechniques nationaux (Comités nationaux de la CEI). La CEI a pour objet de favoriser la coopération internationale pour toutes les questions de normalisation dans les domaines de l'électricité et de l'électronique. A cet effet, la CEI, entre autres activités, publie des Normes internationales.Leur élaboration est confiée à des comités d'études, aux travaux desquels tout Comité national intéressé par le sujet traité peut participer. Les organisations internationales, gouvernementales et non gouvernementales, en liaison avec la CEI, participent également aux travaux. La CEI collabore étroitement avec l'Organisation Internationale de Normalisation (ISO), selon des conditions fixées par accord entre les deux organisations.2)Les décisions ou accords officiels de la CEI concernant les questions techniques représentent, dans la mesuredu possible un accord international sur les sujets étudiés, étant donné que les Comités nationaux intéressés sont représentés dans chaque comité d’études.3)Les documents produits se présentent sous la forme de recommandations internationales. Ils sont publiéscomme normes, rapports techniques ou guides et agréés comme tels par les Comités nationaux.4)Dans le but d'encourager l'unification internationale, les Comités nationaux de la CEI s'engagent à appliquer defaçon transparente, dans toute la mesure possible, les Normes internationales de la CEI dans leurs normes nationales et régionales. Toute divergence entre la norme de la CEI et la norme nationale ou régionale correspondante doit être indiquée en termes clairs dans cette dernière.5)La CEI n’a fixé aucune procédure concernant le marquage comme indication d’approbation et sa responsabilitén’est pas engagée quand un matériel est déclaré conforme à l’une de ses normes.6) L’attention est attirée sur le fait que certains des éléments de la présente Norme internationale peuvent fairel’objet de droits de propriété intellectuelle ou de droits analogues. La CEI ne saurait être tenue pour responsable de ne pas avoir identifié de tels droits de propriété et de ne pas avoir signalé leur existence.La Norme internationale CEI 61854 a été établie par le comité d'études 11 de la CEI: Lignes aériennes.Le texte de cette norme est issu des documents suivants:FDIS Rapport de vote11/141/FDIS11/143/RVDLe rapport de vote indiqué dans le tableau ci-dessus donne toute information sur le vote ayant abouti à l'approbation de cette norme.L’annexe A fait partie intégrante de cette norme.Les annexes B, C et D sont données uniquement à titre d’information.61854 © IEC:1998– 7 –INTERNATIONAL ELECTROTECHNICAL COMMISSION––––––––––OVERHEAD LINES –REQUIREMENTS AND TESTS FOR SPACERSFOREWORD1)The IEC (International Electrotechnical Commission) is a worldwide organization for standardization comprisingall national electrotechnical committees (IEC National Committees). The object of the IEC is to promote international co-operation on all questions concerning standardization in the electrical and electronic fields. To this end and in addition to other activities, the IEC publishes International Standards. Their preparation is entrusted to technical committees; any IEC National Committee interested in the subject dealt with may participate in this preparatory work. International, governmental and non-governmental organizations liaising with the IEC also participate in this preparation. The IEC collaborates closely with the International Organization for Standardization (ISO) in accordance with conditions determined by agreement between the two organizations.2)The formal decisions or agreements of the IEC on technical matters express, as nearly as possible, aninternational consensus of opinion on the relevant subjects since each technical committee has representation from all interested National Committees.3)The documents produced have the form of recommendations for international use and are published in the formof standards, technical reports or guides and they are accepted by the National Committees in that sense.4)In order to promote international unification, IEC National Committees undertake to apply IEC InternationalStandards transparently to the maximum extent possible in their national and regional standards. Any divergence between the IEC Standard and the corresponding national or regional standard shall be clearly indicated in the latter.5)The IEC provides no marking procedure to indicate its approval and cannot be rendered responsible for anyequipment declared to be in conformity with one of its standards.6) Attention is drawn to the possibility that some of the elements of this International Standard may be the subjectof patent rights. The IEC shall not be held responsible for identifying any or all such patent rights. International Standard IEC 61854 has been prepared by IEC technical committee 11: Overhead lines.The text of this standard is based on the following documents:FDIS Report on voting11/141/FDIS11/143/RVDFull information on the voting for the approval of this standard can be found in the report on voting indicated in the above table.Annex A forms an integral part of this standard.Annexes B, C and D are for information only.– 8 –61854 © CEI:1998LIGNES AÉRIENNES –EXIGENCES ET ESSAIS APPLICABLES AUX ENTRETOISES1 Domaine d'applicationLa présente Norme internationale s'applique aux entretoises destinées aux faisceaux de conducteurs de lignes aériennes. Elle recouvre les entretoises rigides, les entretoises flexibles et les entretoises amortissantes.Elle ne s'applique pas aux espaceurs, aux écarteurs à anneaux et aux entretoises de mise à la terre.NOTE – La présente norme est applicable aux pratiques de conception de lignes et aux entretoises les plus couramment utilisées au moment de sa rédaction. Il peut exister d'autres entretoises auxquelles les essais spécifiques décrits dans la présente norme ne s'appliquent pas.Dans de nombreux cas, les procédures d'essai et les valeurs d'essai sont convenues entre l'acheteur et le fournisseur et sont énoncées dans le contrat d'approvisionnement. L'acheteur est le mieux à même d'évaluer les conditions de service prévues, qu'il convient d'utiliser comme base à la définition de la sévérité des essais.La liste des informations techniques minimales à convenir entre acheteur et fournisseur est fournie en annexe A.2 Références normativesLes documents normatifs suivants contiennent des dispositions qui, par suite de la référence qui y est faite, constituent des dispositions valables pour la présente Norme internationale. Au moment de la publication, les éditions indiquées étaient en vigueur. Tout document normatif est sujet à révision et les parties prenantes aux accords fondés sur la présente Norme internationale sont invitées à rechercher la possibilité d'appliquer les éditions les plus récentes des documents normatifs indiqués ci-après. Les membres de la CEI et de l'ISO possèdent le registre des Normes internationales en vigueur.CEI 60050(466):1990, Vocabulaire Electrotechnique International (VEI) – Chapitre 466: Lignes aériennesCEI 61284:1997, Lignes aériennes – Exigences et essais pour le matériel d'équipementCEI 60888:1987, Fils en acier zingué pour conducteurs câblésISO 34-1:1994, Caoutchouc vulcanisé ou thermoplastique – Détermination de la résistance au déchirement – Partie 1: Eprouvettes pantalon, angulaire et croissantISO 34-2:1996, Caoutchouc vulcanisé ou thermoplastique – Détermination de la résistance au déchirement – Partie 2: Petites éprouvettes (éprouvettes de Delft)ISO 37:1994, Caoutchouc vulcanisé ou thermoplastique – Détermination des caractéristiques de contrainte-déformation en traction61854 © IEC:1998– 9 –OVERHEAD LINES –REQUIREMENTS AND TESTS FOR SPACERS1 ScopeThis International Standard applies to spacers for conductor bundles of overhead lines. It covers rigid spacers, flexible spacers and spacer dampers.It does not apply to interphase spacers, hoop spacers and bonding spacers.NOTE – This standard is written to cover the line design practices and spacers most commonly used at the time of writing. There may be other spacers available for which the specific tests reported in this standard may not be applicable.In many cases, test procedures and test values are left to agreement between purchaser and supplier and are stated in the procurement contract. The purchaser is best able to evaluate the intended service conditions, which should be the basis for establishing the test severity.In annex A, the minimum technical details to be agreed between purchaser and supplier are listed.2 Normative referencesThe following normative documents contain provisions which, through reference in this text, constitute provisions of this International Standard. At the time of publication of this standard, the editions indicated were valid. All normative documents are subject to revision, and parties to agreements based on this International Standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. Members of IEC and ISO maintain registers of currently valid International Standards.IEC 60050(466):1990, International Electrotechnical vocabulary (IEV) – Chapter 466: Overhead linesIEC 61284:1997, Overhead lines – Requirements and tests for fittingsIEC 60888:1987, Zinc-coated steel wires for stranded conductorsISO 34-1:1994, Rubber, vulcanized or thermoplastic – Determination of tear strength – Part 1: Trouser, angle and crescent test piecesISO 34-2:1996, Rubber, vulcanized or thermoplastic – Determination of tear strength – Part 2: Small (Delft) test piecesISO 37:1994, Rubber, vulcanized or thermoplastic – Determination of tensile stress-strain properties– 10 –61854 © CEI:1998 ISO 188:1982, Caoutchouc vulcanisé – Essais de résistance au vieillissement accéléré ou à la chaleurISO 812:1991, Caoutchouc vulcanisé – Détermination de la fragilité à basse températureISO 815:1991, Caoutchouc vulcanisé ou thermoplastique – Détermination de la déformation rémanente après compression aux températures ambiantes, élevées ou bassesISO 868:1985, Plastiques et ébonite – Détermination de la dureté par pénétration au moyen d'un duromètre (dureté Shore)ISO 1183:1987, Plastiques – Méthodes pour déterminer la masse volumique et la densitérelative des plastiques non alvéolairesISO 1431-1:1989, Caoutchouc vulcanisé ou thermoplastique – Résistance au craquelage par l'ozone – Partie 1: Essai sous allongement statiqueISO 1461,— Revêtements de galvanisation à chaud sur produits finis ferreux – Spécifications1) ISO 1817:1985, Caoutchouc vulcanisé – Détermination de l'action des liquidesISO 2781:1988, Caoutchouc vulcanisé – Détermination de la masse volumiqueISO 2859-1:1989, Règles d'échantillonnage pour les contrôles par attributs – Partie 1: Plans d'échantillonnage pour les contrôles lot par lot, indexés d'après le niveau de qualité acceptable (NQA)ISO 2859-2:1985, Règles d'échantillonnage pour les contrôles par attributs – Partie 2: Plans d'échantillonnage pour les contrôles de lots isolés, indexés d'après la qualité limite (QL)ISO 2921:1982, Caoutchouc vulcanisé – Détermination des caractéristiques à basse température – Méthode température-retrait (essai TR)ISO 3417:1991, Caoutchouc – Détermination des caractéristiques de vulcanisation à l'aide du rhéomètre à disque oscillantISO 3951:1989, Règles et tables d'échantillonnage pour les contrôles par mesures des pourcentages de non conformesISO 4649:1985, Caoutchouc – Détermination de la résistance à l'abrasion à l'aide d'un dispositif à tambour tournantISO 4662:1986, Caoutchouc – Détermination de la résilience de rebondissement des vulcanisats––––––––––1) A publierThis is a preview - click here to buy the full publication61854 © IEC:1998– 11 –ISO 188:1982, Rubber, vulcanized – Accelerated ageing or heat-resistance testsISO 812:1991, Rubber, vulcanized – Determination of low temperature brittlenessISO 815:1991, Rubber, vulcanized or thermoplastic – Determination of compression set at ambient, elevated or low temperaturesISO 868:1985, Plastics and ebonite – Determination of indentation hardness by means of a durometer (Shore hardness)ISO 1183:1987, Plastics – Methods for determining the density and relative density of non-cellular plasticsISO 1431-1:1989, Rubber, vulcanized or thermoplastic – Resistance to ozone cracking –Part 1: static strain testISO 1461, — Hot dip galvanized coatings on fabricated ferrous products – Specifications1)ISO 1817:1985, Rubber, vulcanized – Determination of the effect of liquidsISO 2781:1988, Rubber, vulcanized – Determination of densityISO 2859-1:1989, Sampling procedures for inspection by attributes – Part 1: Sampling plans indexed by acceptable quality level (AQL) for lot-by-lot inspectionISO 2859-2:1985, Sampling procedures for inspection by attributes – Part 2: Sampling plans indexed by limiting quality level (LQ) for isolated lot inspectionISO 2921:1982, Rubber, vulcanized – Determination of low temperature characteristics –Temperature-retraction procedure (TR test)ISO 3417:1991, Rubber – Measurement of vulcanization characteristics with the oscillating disc curemeterISO 3951:1989, Sampling procedures and charts for inspection by variables for percent nonconformingISO 4649:1985, Rubber – Determination of abrasion resistance using a rotating cylindrical drum deviceISO 4662:1986, Rubber – Determination of rebound resilience of vulcanizates–––––––––1) To be published.。
r8s使用指南
r8s使用指南中国科学院植物研究所张金龙编译zhangjl@前言r8s是美国加利福尼亚大学戴维斯分校的进化生物学家Mike Sanderson编写的用于估算进化树分化时间的软件,在进化生物学、分子生物地理学等学科有着广泛的应用,已经成为估算分化时间不可或缺的软件之一。
该软件中的一些方法如NPRS和PL是软件作者最先提出的,目前在同类的其他软件中还难以实现。
R8s的运行平台为MacOS和Linux,在国内应用的还不多,也难以找到中文的练习资料和说明。
本文基于当前版本r8s 1.7.1,参照其说明书,介绍该软件在Linux下的安装和操作,并对其模块的功能和选项进行简要的说明。
译者于北京香山2010年1月23日目录一r8s下载与安装 (1)下载 (1)安装 (1)1 在MacOS上 (1)2 在Linux上(以Ubuntu 9.0为例) (1)(1)下载源程序 (1)(2)解压缩 (1)(3) 源代码的编译 (1)注:g77编译器的安装 (1)3 Windows用户 (2)二程序运行 (2)1 在Linux中(Ubuntu linux 或PHYLIS) (2)2 在WindowXP中运行 (3)程序运行模式 (3)1 交互模式 (3)2 批处理模式 (3)三命令行说明 (4)blformat命令: 进化树的基本信息 (4)mrca命令为节点定名 (5)fixage命令:设定节点的分化时间 (5)constrain命令:限定节点的分化时间 (5)divtime 命令分化时间估算 (5)showage 显示分化时间和分化速率: (6)describe 显示进化树及树的说明 (6)set 命令 (7)calibrate 时间校对 (7)profile 从多个树中提取某个节点的信息 (7)rrlike 检验进化速率 (7)四数据处理过程中的建议 (7)关于进化模型的说明 (7)局部进化时间模型localmodel (7)对于获得时间的建议 (8)关于时间估算的bootstrap的方法 (8)改错 (8)五实例分析 (8)附录命令参考 (11)blformat (11)calibrate (11)cleartrees (11)collapse (11)constrain (11)describe (11)divtime (11)execute (12)fixage (12)localmodel (12)mrca (12)profile (12)prune (12)quit (12)reroot (12)rrlike (12)set (12)showage (13)unfixage (13)mrp (13)bd (14)一r8s下载与安装下载r8s的下载网址/r8s//r8s/r8s1.71.dist.tar.Z安装1 在MacOS上在MacOS上运行,在UNIX shell中运行已经编译好的可执行文件即可。
新一代计算机——DNA计算机的研究及开发
新一代计算机——DNA计算机的研究及开发计算机是人类科技进步的杰出成果之一,它的发展从最初的机械计算到电子计算机、智能计算机、量子计算机等,已经跨越了数个世纪。
然而,计算机领域仍然存在许多挑战和难题,其中之一就是如何提高计算速度和存储容量,以满足人类对信息处理和存储的需求。
近年来,生物技术逐渐成为计算机业界新的研究方向,新一代计算机——DNA计算机应运而生。
本文将探讨DNA 计算机的研究及开发现状和前景。
DNA计算机是什么?DNA计算机起源于1994年,由阿迪耶夫(Leonard Adleman)提出。
他提出了一个新颖的思想,即利用分子生物学领域的DNA (脱氧核糖核酸)分子来进行计算。
DNA分子是生物体内存储遗传信息的基本分子,具有极高的存储密度和大容量,而且具有自我复制、自组装等自主性质。
这些特性使得DNA分子被认为是进行复杂计算的理想介质。
DNA计算机便是利用这些特性,将DNA 分子作为信息存储和计算的基本单位,开发出一种新型计算机。
DNA计算机的基本工作原理DNA计算机的基本工作原理是将计算问题转化为DNA分子的相关问题,再通过实验方法对DNA分子进行处理,最终得到正确答案。
例如,某个复杂计算问题A可以转化为某种DNA分子序列,然后在实验室中进行DNA分子的处理和操作,最终得到计算问题A的正确答案。
这些实验操作包括DNA分子的合成、分离、连接、分子修饰等,同时也包括生物学实验等其他技术手段。
整个计算过程需要经过多个步骤,其中许多步骤需要复杂的实验操作和数据分析。
因此,DNA计算机的设计与操作需要涉及许多学科领域,如分子生物学、生物信息学、电子工程、计算机科学等。
DNA计算机的优点和应用DNA计算机相对于传统计算机具有许多优点。
首先,由于DNA分子的极小尺寸和高存储密度,DNA计算机可以在极小的空间内存储大量数据和信息。
其次,DNA计算机具有自主性、并行性、大容量、可以通过分子间相互作用完成计算任务等特点,可以在某些特定的计算任务中显示出超越传统计算机的优越性。
MCP 70-528 考试题库
D. Add the following code segment to the Web site's configuration file.<pages theme="ThemeName" />Answer: C2. You create a Web Form. The Web Form allows users to calculate values and display the results in a label named lblResults.You need to capture all unhandled exceptions on the Web Form through the Error event. The Error event must capture each unhandled exception and display it on the Web Form.Which code segment should you use?A. Protected Sub Page_Error(ByVal sender As Object, _ByVal e As System.EventArgs) Handles Me.ErrorlblResults.Text = e.ToString()e = NothingEnd SubB. Protected Sub Page_Error(ByVal sender As Object, _ByVal e As System.EventArgs) Handles Me.Error?lblResults.Text = Server.GetLastError().ToString()Server.ClearError()End SubC. Protected Sub Page_Error(ByVal sender As Object, _ByVal e As System.EventArgs) Handles Me.Error?Response.Write(e.ToString())e = NothingEnd SubD. Protected Sub Page_Error(ByVal sender As Object, _ByVal e As System.EventArgs) Handles Me.ErrorResponse.Write(Server.GetLastError().ToString())Server.ClearError()End SubAnswer: D3. You write a Web application. This application must support multiple languages. You store the localized strings in the application as resources. You want these resources to be accessed according to a users language preference. You create the following resource files in the App_GlobalResources folder of your application.Each resource file stores a localized version of the following strings: Name, E-mail, Address, and Phone. You create a Web Form that contains one label for each of these strings.You need to ensure that the correct localized version of each string is displayed in each label, according to a users language preference. What should you do?A. Add the following configuration section to the Web.config file.<globalization culture="Auto" />B. Set the directive for each page in your site as follows:<%@ Page UICulture="Auto" %>C. Add the following code segment to the pages load event.lblName.Text = @"{myStrings}Name";lblAddress.Text = @"{myStrings}Address";lblEmail.Text = @"{myStrings}Email";lblPhone.Text = @"{myStrings}Phone";D. Add the following code segment to the pages load event.lblName.Text = ;lblAddress.Text = Resources.myStrings.Address;lblEmail.Text = Resources.myStrings.Email;lblPhone.Text = Resources.myStrings.Phone;Answer: D4. You create a Web Form. The Web Form allows users to calculate values and display the results in a label named lblResults.You need to capture all unhandled exceptions on the Web Form through the Error event. The Error event must capture each unhandled exception and display it on the Web Form.Which code segment should you use?A. protected void Page_Error(object sender, EventArgs e) {lblResults.Text = e.ToString();e=null;}B. protected void Page_Error(object sender, EventArgs e) {lblResults.Text = Server.GetLastError().ToString();Server.ClearError();}C. protected void Page_Error(object sender, EventArgs e) {Response.Write(e.ToString());e=null;}D. protected void Page_Error(object sender, EventArgs e) {Response.Write(Server.GetLastError().ToString());Server.ClearError();}Answer: D5. You create a Web Form. The Web Form uses the FormView control to enable a user to edit a record in the database.When the user clicks the Update button on the FormView control, the application must validate that the user has entered data in all of the fields.You need to ensure that the Web Form does not update if the user has not entered data in all of the fields.Which code segment should you use?A. protected void FormView1_ItemUpdating(object sender, FormViewUpdateEventArgs e) {foreach (DictionaryEntry entry in e.Keys) {if (entry.Value.ToString() == System.String.Empty) {e.Cancel = true;return;}}}B. protected void FormView1_ItemUpdated(object sender, FormViewUpdatedEventArgs e) {foreach (DictionaryEntry entry in e.NewValues) {if (entry.Value.Equals("")) {e.KeepInEditMode = true;return;}}}C. protected void FormView1_ItemUpdating(object sender, FormViewUpdateEventArgs e) {foreach (DictionaryEntry entry in e.NewValues) {if (entry.Value.Equals("")) {e.Cancel = true;return;}}}D. protected void FormView1_ItemUpdated(object sender, FormViewUpdatedEventArgs e) {foreach (DictionaryEntry entry in e.Keys) {if (entry.Value.ToString() == System.String.Empty) {e.KeepInEditMode = true;return;}}}Answer: C6. You are creating a Web Form. The Web Form allows users to rename or delete products in a list. You create a DataTable named dtProducts that is bound to a GridView. DataTable has the following four rows.The user utilizes a Web Form to delete the first product.You need to set the RowStateFilter property of the DataTables DefaultView so that only products that have not been deleted are shown. To which value should you set the DataTabless DefaultView.RowStateFilter?A. DataViewRowState.ModifiedOriginalB. DataViewRowState.ModifiedCurrentC. DataViewRowState.CurrentRowsD. DataViewRowState.AddedAnswer: C7. You are creating a Web Form. You write the following code segment to create a SqlCommand object.You need to display the number of customers in the Customers table.Which two code segments can you use to achieve this goal? (Each correct answer presents a complete solution. Choose two.)A. object customerCount = cmd.ExecuteScalar();lblCompanyName.Text = customerCount.ToString();B. int customerCount = cmd.ExecuteNonQuery();lblCompanyName.Text = customerCount.ToString();C. SqlDataReader dr = cmd.ExecuteReader();dr.Read();lblCompanyName.Text = dr[0].ToString();D. SqlDataReader dr = cmd.ExecuteReader();dr.Read();lblCompanyName.Text = dr.ToString();Answer: A AND C8. You have an SQL query that takes one minute to execute. You use the following code segment to execute the SQL query asynchronously.IAsyncResult ar = cmd.BeginExecuteReader();You need to execute a method named DoWork() that takes one second to run while the SQL query is executing. DoWork() must run as many times as possible while the SQL query is executing.Which code segment should you use?A. while (ar.AsyncWaitHandle == null) {DoWork();}dr = cmd.EndExecuteReader(ar);B. while (!ar.IsCompleted) {DoWork();}dr = cmd.EndExecuteReader(ar);C. while (Thread.CurrentThread.ThreadState == ThreadState.Running) {DoWork();}dr = cmd.EndExecuteReader(ar);D. while (!ar.AsyncWaitHandle.WaitOne()) {DoWork();}dr = cmd.EndExecuteReader(ar);Answer: B9. Your Web site processes book orders. One of the application methods contains the following code segment.You need to remove the discount element from X mlDocument.Which two code segments can you use to achieve this goal? (Each correct answer presents a complete solution. Choose two.)A. Dim root As X mlNode = doc.DocumentElementroot.RemoveChild(root.FirstChild)B. Dim root As X mlNode = doc.DocumentElementroot.RemoveChild(root.SelectSingleNode("discount"))C.doc.RemoveChild(doc.FirstChild)D.doc.DocumentElement.RemoveChild(doc.FirstChild)Answer: B AND A10. You are creating a DataTable. You use the following code segment to create the DataTable. (Line numbers are included for reference only.)You need to ensure that the Total column is set to the value of the Price column multiplied by the Quantity column when new rows are added or changed.What should you do?A. Add the following code segment after line 05.dc.ExtendedProperties("Total") = "Price * Quantity"B. Add the following code segment after line 05.dc.Expression = "Price * Quantity"C. Write an event handler for the DataTable's TableNewRow event that updates the row's Total.D. Write an event handler for the DataTable's ColumnChanged event that updates the row's Total.Answer: B11. You create a Web Form that displays a GridView. The GridViews data source is a DataSet named dsOrders. The DataSet contains two DataTables named Orders and OrderDetails. You create a relation between the two DataTables using the following code segment. (Line numbers are included for reference only.)You need to find the cause of the exception being raised in line 05.What should you do?A. Ensure that the child column and the parent column have the same names.B. Ensure that the child table and the parent table have the same names.C. Ensure that the child column and the parent column have the same data types.D. Ensure that each row in the child table has a corresponding row in the parent table.E. Ensure that the tables have an explicit relationship defined by a foreign key constraint in the database.Answer: C12. Your Web site uses custom Themes. Your Web site must support additional Themes based on the user's company name.The company name is set when a user logs on to the Web site. The company's Theme name is stored in a variable named ThemeName. You need to use this variable to dynamically set the Web site's Theme.What should you do?A. Add the following code segment to the markup source of each page on the Web site.<%@ Page Theme="ThemeName" ... %>B. Add the following code segment to the Load event of each page on the Web site.Page.Theme = ThemeNameC. Add the following code segment to the PreInit event of each page on the Web site.Page.Theme = ThemeNameD. Add the following code segment to the Web site's configuration file.<pages theme="ThemeName" />Answer: C13. You are creating a Web Form. The Web Form allows users to rename or delete products in a list. You create a DataTable named dtProducts that is bound to a GridView. DataTable has the following four rows.The user utilizes a Web Form to delete the first product.You need to set the RowStateFilter property of the DataTables DefaultView so that only products that have not been deleted are shown. To which value should you set the DataTabless DefaultView.RowStateFilter?A. DataViewRowState.ModifiedOriginal;B. DataViewRowState.ModifiedCurrent;C. DataViewRowState.CurrentRows;D. DataViewRowState.Added;Answer: C14. Your Web site processes book orders. One of the application methods contains the following code segment.You need to remove the discount element from X mlDocument.Which two code segments can you use to achieve this goal? (Each correct answer presents a complete solution. Choose two.)A.X mlNode root = doc.DocumentElement;root.RemoveChild(root.FirstChild);B.X mlNode root = doc.DocumentElement;root.RemoveChild(root.SelectSingleNode("discount"));C.doc.RemoveChild(doc.FirstChild);D.doc.DocumentElement.RemoveChild(doc.FirstChild);Answer: B AND A15. You write a Web application. This application must support multiple languages. You store the localized strings in the application as resources. You want these resources to be accessed according to a users language preference. You create the following resource files in the App_GlobalResources folder of your application.Each resource file stores a localized version of the following strings: Name, E-mail, Address, and Phone. You create a Web Form that contains one label for each of these strings.You need to ensure that the correct localized version of each string is displayed in each label, according to a users language preference. What should you do?A. Add the following configuration section to the Web.config file.<globalization culture="Auto" />B. Set the directive for each page in your site as follows:<%@ Page UICulture="Auto" %>C. Add the following code segment to the pages load event.lblName.Text = "{myStrings}Name"lblAddress.Text = "{myStrings}Address"lblEmail.Text = "{myStrings}Email"lblPhone.Text = "{myStrings}Phone"D. Add the following code segment to the pages load event.lblName.Text = lblAddress.Text = Resources.myStrings.AddresslblEmail.Text = Resources.myStrings.EmaillblPhone.Text = Resources.myStrings.PhoneAnswer: D16. You are creating a DataTable. You use the following code segment to create the DataTable. (Line numbers are included for reference only.)You need to ensure that the Total column is set to the value of the Price column multiplied by the Quantity column when new rows are added or changed.What should you do?A. Add the following code segment after line 05.dc.ExtendedProperties["Total"] = "Price * Quantity";B. Add the following code segment after line 05.dc.Expression = "Price * Quantity";C. Write an event handler for the DataTable's TableNewRow event that updates the row's Total.D. Write an event handler for the DataTable's ColumnChanged event that updates the row's Total.Answer: B17. You create a Web Form. The Web Form uses the FormView control to enable a user to edit a record in the database.When the user clicks the Update button on the FormView control, the application must validate that the user has entered data in all of the fields.You need to ensure that the Web Form does not update if the user has not entered data in all of the fields.Which code segment should you use?A. Protected Sub FormView1_ItemUpdating(ByVal sender As Object, _ByVal e As System.Web.UI.WebControls.FormViewUpdateEventArgs) _Handles FormView1.ItemUpdatingDim entry As DictionaryEntryFor Each entry In e.KeysIf entry.Value.ToString() = System.String.Empty Thene.Cancel = TrueReturnEnd IfNext entryEnd SubB. Protected Sub FormView1_ItemUpdated(ByVal sender As Object, _ByVal e As System.Web.UI.WebControls.FormViewUpdatedEventArgs) _Handles FormView1.ItemUpdatedDim entry As DictionaryEntryFor Each entry In e.NewValuesIf entry.Value.Equals("") Thene.KeepInEditMode = TrueReturnEnd IfNext entryEnd SubC. Protected Sub FormView1_ItemUpdating(ByVal sender As Object, _ByVal e As System.Web.UI.WebControls.FormViewUpdateEventArgs) _Handles FormView1.ItemUpdatingDim entry As DictionaryEntryFor Each entry In e.NewValuesIf entry.Value.Equals("") Thene.Cancel = TrueReturnEnd IfNext entryEnd SubD. Protected Sub FormView1_ItemUpdated(ByVal sender As Object, _ByVal e As System.Web.UI.WebControls.FormViewUpdatedEventArgs) _Handles FormView1.ItemUpdatedDim entry As DictionaryEntryFor Each entry In e.KeysIf entry.Value.ToString() = System.String.Empty Thene.KeepInEditMode = TrueReturnEnd IfNext entryEnd SubAnswer: C18. You create a Web Form that displays a GridView. The GridView's data source is a DataSet named dsOrders. The DataSet contains two DataTables named Orders and OrderDetails. You create a relation between the two DataTables using the following code segment. (Line numbers are included for reference only.)You need to find the cause of the exception being raised in line 05.What should you do?A. Ensure that the child column and the parent column have the same names.B. Ensure that the child table and the parent table have the same names.C. Ensure that the child column and the parent column have the same data types.D. Ensure that each row in the child table has a corresponding row in the parent table.E. Ensure that the tables have an explicit relationship defined by a foreign key constraint in the database.Answer: C19. You have an SQL query that takes one minute to execute. You use the following code segment to execute the SQL query asynchronously.Dim ar As IAsyncResult = cmd.BeginExecuteReader()You need to execute a method named DoWork() that takes one second to run while the SQL query is executing. DoWork() must run as many times as possible while the SQL query is executing.Which code segment should you use?A. While ar.AsyncWaitHandle Is NothingDoWork()End Whiledr = cmd.EndExecuteReader(ar)B. While Not ar.IsCompletedDoWork()End Whiledr = cmd.EndExecuteReader(ar)C. While Thread.CurrentThread.ThreadState = ThreadState.RunningDoWork()End Whiledr = cmd.EndExecuteReader(ar)D. While Not ar.AsyncWaitHandle.WaitOne()DoWork()End Whiledr = cmd.EndExecuteReader(ar)Answer: B20. You are creating a Web Form. You write the following code segment to create a SqlCommand object.You need to display the number of customers in the Customers table.Which two code segments can you use to achieve this goal? (Each correct answer presents a complete solution. Choose two.)A. Dim customerCount As Object = cmd.ExecuteScalar()lblCompanyName.Text = customerCount.ToString()B. Dim customerCount As Integer = cmd.ExecuteNonQuery()lblCompanyName.Text = customerCount.ToString()。
一种从大熊猫粪便中提取DNA的改进方法
动物学报49 (5) :670~674 , 2003Acta Zoologica S i nica一种从大熊猫粪便中提取D NA 的改进方法3钟华①赖旭龙②魏荣平③刘中来①33( ①华中师范大学生命科学学院, 武汉430079)( ②中国地质大学地球科学学院, 武汉430074) ( ③中国保护大熊猫研究中心, 四川卧龙623006)摘要本研究描述一个改进的方法, 使从大熊猫粪便中提取DNA 用于PCR 扩增变得更加容易。
在粪便DNA 的提取过程中采用一个新的预处理方法, 将粪便用预冷的丙酮洗2~3 次, 除去粪便中含有的大量PCR 抑制物, 然后用蛋白酶K 裂解、酚- 氯仿抽提, 能提取到纯度很高的DNA 供PCR 扩增。
本实验PCR 扩增了大熊猫脑源性神经营养因子(BDNF) 基因和线粒体细胞色素 b 基因片段, 并进行测序分析, 证实了提取的可靠性。
对比本方法和未经丙酮预处理的方法提取的DNA 进行PCR 扩增, 前者的扩增结果明显优于后者[ 动物学报49 (5) : 670~674 , 2003 ] 。
关键词大熊猫粪便DNA 丙酮DNA 抽提非损伤性取样An improved protocol for D NA extraction from the faeces of the giant panda 3 ZHON G Hua ① LA I Xu2Long ② W EI Rong2Ping ③ L IU Zhong2Lai ①33( ①College of L if e S cience , Cent ral China Nor mal U niversit y , W uh an430079 , China)( ②Faculty of Earth Sciences , China U niversit y of Geosciences , W uhan430074 , China)( ③China Cons ervation and Res earch Center f or th e Gi ant Pan da , W olong623006 , S ichuan , China) Abstract An improved method that facilitates the extraction of PCR2compatible faecal DNA from giant pand a’s faeces is described. The method involved a novel preprocessing step in DNA extraction. The faeces was washed two or three timeswith precooled acetone , which removed numerous potential PCR inhibitors , and then digested with proteinase K. The DNA was purified with phenol/ chloroform. The faecal DNA obtained was sufficiently pure to support reliable amplifica2 tion , and was applied as template DNA to amplify a portion of the giant panda brain derived neurotrophic factor (BDNF) gene and mitochondrial cytochrome b gene. The sequenced results of PCR products confirmed that the extracted DNA was from the giant panda. Comparison with the PCR products demonstrated that the faecal DNA extracted b y the improved protocol was better than the faecal DNA extracted without acetone preprocessing. [ Acta Zoologica S inica 49 (5) : 670 - 674 , 2003 ] .K ey words Giant panda ( A il uropoda melanoleuca) , Faecal DNA , Acetone , DNA extraction , Noninvasive sampling在大熊猫的遗传多样性、种群数量调查、进化和分类、亲子鉴定等研究中, DNA 分析是重要的研究手段。
ptahdao风险提示函
Ptahdao风险提示函1. 引言本风险提示函旨在向投资者提供关于Ptahdao项目的风险提示信息,帮助投资者全面了解并评估项目的风险与收益。
请投资者在决策投资前详细阅读本函并谨慎考虑。
2. 项目概述Ptahdao是一个基于区块链技术的去中心化金融(DeFi)项目。
该项目旨在提供一种安全、高效、透明的金融服务平台,为用户提供借贷、存款、理财等功能。
投资者可以通过购买Ptahdao代币(PTD)来参与项目,并享受相应的收益。
3. 风险提示3.1 技术风险Ptahdao项目依赖于区块链技术的稳定和安全性。
然而,区块链技术仍处于发展阶段,存在许多技术风险。
投资者应意识到以下可能的技术风险:•网络攻击风险:由于区块链的开放性和去中心化特性,Ptahdao项目可能面临来自黑客和恶意攻击者的网络攻击风险。
这可能导致投资者的资金损失。
•智能合约漏洞风险:智能合约是Ptahdao项目的核心组成部分,但智能合约可能存在漏洞。
一旦智能合约出现漏洞,攻击者可能利用漏洞进行恶意操作,导致投资者的资金损失。
•网络拥堵风险:区块链网络可能面临拥堵问题,导致交易延迟和高额的交易手续费。
投资者应该注意网络拥堵可能对交易执行和用户体验产生的不利影响。
3.2 市场风险Ptahdao代币(PTD)的价格受市场供求关系的影响,可能存在以下市场风险:•价格波动风险:Ptahdao代币的价格可能受到市场供求关系、投资者情绪、宏观经济因素等多种因素的影响,可能出现剧烈波动。
投资者应该意识到价格波动可能导致投资本金的损失。
•流动性风险:Ptahdao代币市场可能存在流动性不足的情况,投资者在买卖代币时可能面临成交困难和价格滑点的风险。
3.3 法律合规风险Ptahdao项目的运营和发展可能受到法律法规的影响,投资者应该注意以下法律合规风险:•监管风险:区块链技术和加密货币行业的监管环境尚不明确,可能存在政策风险和监管风险。
投资者应该关注相关法律法规的变化和政策风险对项目的影响。
Operating System
Operating SystemAn operating system (OS) is a set of programs that manage computer hardware resources and provide common services for application software. The operating system is a vital component of the system software in a computer system. Application programs require an operating system which are usually separate programs, but can be combined in simple systems.Time-sharing operating systems schedule tasks for efficient use of the system and may also include accounting for cost allocation of processor time, mass storage, printing, and other resources.For hardware functions such as input and output and memory allocation, the operating system acts as an intermediary between application programs and the computer hardware,[1][2] although the application code is usually executed directly by the hardware and will frequently make a system call to an OS function or be interrupted by it. Operating systems are found on almost any device that contains a computer—from cellular phones and video game consoles to supercomputers and web servers.Examples of popular modern operating systems include Android, BSD, iOS,Linux, Mac OS X, Microsoft Windows,[3]Windows Phone, and IBM z/OS. All these, except Windows and z/OS, share roots in UNIX.UNIX and UNIX-like operating systemsKen Thompson wrote B, mainly based on BCPL, which he used to write Unix, based on his experience in the MULTICS project. B was replaced by C, and Unix developed into a large,complex family of inter-related operating systems which have been influential in every modern operating system (see History).The UNIX-like family is a diverse group of operating systems, with several major sub-categories including System V, BSD, and GNU/Linux. The name "UNIX" is a trademark of The Open Group which licenses it for use with any operating system that has been shown to conform to their definitions. "UNIX-like" is commonly used to refer to the large set of operating systems which resemble the original UNIX.Unix-like systems run on a wide variety of computer architectures. They are used heavily for servers in business, as well as workstations in academic and engineering environments. Free UNIX variants, such as GNU/Linux and BSD, are popular in these areas.Four operating systems are certified by the The Open Group (holder of the Unix trademark) as Unix. HP's HP-UX and IBM's AIX are both descendants of the original System V Unix and are designed to run only on their respective vendor's hardware. In contrast, Sun Microsystems's Solaris Operating System can run on multiple types of hardware, including x86and Sparc servers, and PCs. Apple's Mac OS X, a replacement for Apple's earlier (non-Unix) Mac OS, is a hybrid kernel-based BSD variant derived from NeXTSTEP, Mach, and FreeBSD.Unix interoperability was sought by establishing the POSIX standard. The POSIX standard can be applied to any operating system, although it was originally created for various Unix variants.Mac OS XThe standard user interface of Mac OS XMain article: Mac OS XMac OS X is a line of open core graphical operating systems developed, marketed, and sold by Apple Inc., the latest of which is pre-loaded on all currently shipping Macintosh computers. Mac OS X is the successor to the original Mac OS, which had been Apple's primary operating system since 1984. Unlike its predecessor, Mac OS X is a UNIX operating system built on technology that had been developed at NeXT through the second half of the 1980s and up until Apple purchased the company in early 1997.The operating system was first released in 1999 as Mac OS X Server 1.0, with a desktop-oriented version (Mac OS X v10.0 "Cheetah") following in March 2001. Since then, six more distinct "client" and "server" editions of Mac OS X have been released, the most recent being OS X 10.8 "Mountain Lion", which was first made available on February 16, 2012 for developers, and to be released to the public late summer 2012. Releases of Mac OS X are named after big cats.The server edition, Mac OS X Server, is architecturally identical to its desktop counterpart but usually runs on Apple's line of Macintosh server hardware. Mac OS X Server includes work group management and administration software tools that provide simplified access to key network services, including a mail transfer agent, a Samba server, an LDAP server, a domain name server, and others. In Mac OS X v10.7 Lion, all server aspects of Mac OS X Server have been integrated into the client version.[5]Linux and GNUUbuntu, desktop Linux distributionAndroid, a popular mobile operating system using the Linux kernelLinux (or GNU/Linux) is a Unix-like operating system that was developed without any actual Unix code, unlike BSD and its variants. Linux can be used on a wide range of devices from supercomputers to wristwatches. The Linux kernel is released under an open source license, so anyone can read and modify its code. It has been modified to run on a large variety of electronics. Although estimates suggest that Linux is used on 1.82% of all personal computers,[6][7]it has been widely adopted for use in servers[8]and embedded systems[9] (such as cell phones). Linux has superseded Unix in most places[which?], and is used on the 10 most powerful supercomputers in the world.[10] The Linux kernel is used in some popular distributions, such as Red Hat, Debian, Ubuntu, Linux Mint and Google's Android.The GNU project is a mass collaboration of programmers who seek to create a completely free and open operating system that was similar to Unix but with completely original code. It was started in 1983 by Richard Stallman, and is responsible for many of the parts of most Linux variants. Thousands of pieces of software for virtually every operating system are licensed under the GNU General Public License. Meanwhile, the Linux kernel began as a side project of Linus Torvalds, a university student from Finland. In 1991, Torvalds began work on it, and posted information about his project on a newsgroup for computer students and programmers. He received a wave of support and volunteers who ended up creating a full-fledged kernel. Programmers from GNU took notice, and members of both projects worked to integrate the finished GNU parts with the Linux kernel in order to create a full-fledged operating system.Google Chrome OSChrome is an operating system based on the Linux kernel and designed by Google. Since Chrome OS targets computer users who spend most of their time on the Internet, it is mainly a web browser with no ability to run applications. It relies on Internet applications (or Web apps) used in the web browser to accomplish tasks such as word processing and media viewing, as well as online storage for storing most files.Microsoft WindowsBootable Windows To Go USB flash driveMicrosoft Windows is a family of proprietary operating systems designed by Microsoft Corporation and primarily targeted to Intel architecture based computers, with an estimated 88.9 percent total usage share on Web connected computers.[7][11][12][13] The newest version is Windows 7 for workstations and Windows Server 2008 R2 for servers. Windows 7 recently overtook Windows XP as most used OS.[14][15][16]Microsoft Windows originated in 1985 as an application running on top of MS-DOS, which was the standard operating system shipped on most Intel architecture personal computers at the time. In 1995, Windows 95 was released which only used MS-DOS as a bootstrap. For backwards compatibility, Win9x could run real-mode MS-DOS[17][18]and 16 bits Windows 3.x[19] drivers. Windows Me, released in 2000, was the last version in the Win9x family. Later versions have all been based on the Windows NTkernel. Current versions of Windows run on IA-32 and x86-64microprocessors, although Windows 8 will support ARM architecture. In the past, Windows NT supported non-Intel architectures.Server editions of Windows are widely used. In recent years, Microsoft has expendedsignificant capital in an effort to promote the use of Windows as a server operating environment. However, Windows' usage on servers is not as widespread as on personal computers, as Windows competes against Linux and BSD for server market share.。
我最喜欢的发明 英语作文
In the vast expanse of human history,countless inventions have shaped the way we live,work,and interact with the world around us.Among these,there is one particular invention that has captured my heart and admiration:the Internet.The Dawn of ConnectivityThe Internet,a global network of interconnected computers,was born out of the need for a robust and flexible communication system.Its origins can be traced back to the late 1960s with the development of ARPANET,a project funded by the U.S.Department of Defense.Over the decades,it has evolved from a tool used primarily by researchers and academics to a ubiquitous presence in the daily lives of billions.The Power of InformationWhat makes the Internet my favorite invention is its unparalleled ability to disseminate information.It has democratized access to knowledge,allowing anyone with a connection to learn about virtually any subject.From the comfort of ones home,one can explore the depths of the ocean,the mysteries of the universe,or the intricacies of ancient civilizations.The Catalyst for InnovationThe Internet has also been a catalyst for innovation,spawning new industries and transforming existing ones.Ecommerce,online education,telemedicine,and social media are just a few examples of how the Internet has revolutionized the way we conduct business and maintain social relationships.The Bridge to Global CommunityMoreover,the Internet has served as a bridge to a global community,breaking down geographical barriers and fostering a sense of interconnectedness.It has enabled people from different cultures and backgrounds to collaborate,share ideas,and build friendships that transcend borders.The Challenges and OpportunitiesWhile the Internet has brought about remarkable benefits,it has also presented challenges, such as issues of privacy,security,and the digital divide.These challenges,however,are opportunities for further innovation and improvement,as we strive to create a more inclusive and secure digital environment.The Future of the InternetAs we look to the future,the potential of the Internet continues to expand with advancements in technology like artificial intelligence,virtual reality,and the Internet of Things.The possibilities are endless,and the Internet remains a testament to human ingenuity and our relentless pursuit of progress.In conclusion,the Internet is more than just an invention it is a living,breathing entity that continues to evolve and shape our world in ways we are only beginning to understand.It is a source of inspiration,a tool for empowerment,and a symbol of our collective potential.For these reasons and more,the Internet holds a special place in my heart as my favorite invention.。
一种新的部分神经进化网络的股票预测(英文)
一种新的部分神经进化网络的股票预测(英文)一种新的部分神经进化网络的股票预测自从股票市场的出现以来,人们一直在寻求能够提前预测股票走势的方法。
许多投资者和研究人员尝试使用各种技术分析工具和模型来预测股票未来的走势,但是股票市场的复杂性和难以预测性使得这变得困难重重。
因此,寻找一种能够准确预测股票走势的方法一直是金融界的热点问题。
近年来,人工智能技术在金融领域的应用日益增多。
其中,神经网络是一种被广泛使用的工具,它可以自动学习和识别模式,并根据所学的模式进行预测。
然而,传统神经网络在预测股票市场方面存在诸多问题,例如过拟合和难以处理大量数据等。
为了克服这些问题,本文提出了一种新的部分神经进化网络(Partial Neural Evolving Network, PNEN)模型来预测股票走势。
PNEN模型将神经网络和进化算法相结合,通过优化和训练来实现更准确的预测结果。
PNEN模型的核心思想是将神经网络的隐藏层拆分为多个小模块,每个小模块只负责处理一部分输入数据。
通过这种方式,模型可以更好地适应不同的市场情况和模式。
同时,采用进化算法来优化模型的参数,可以进一步提高模型的预测性能。
具体而言,PNEN模型包括以下几个步骤:1. 数据准备:从股票市场获取历史交易数据,并对数据进行预处理和归一化处理,以便更好地输入到模型中。
2. 构建模型结构:将神经网络的隐藏层拆分为多个小模块,通过进化算法来确定每个小模块的结构和参数。
进化算法通过优化模型的准确性和稳定性,以获得更好的预测结果。
3. 训练模型:使用历史数据集对模型进行训练,并通过反向传播算法来更新模型的权重和偏置。
同时,通过与进化算法的交互,不断调整模型结构和参数。
4. 预测结果:使用训练好的模型对未来的股票走势进行预测。
通过模型对市场的分析和判断,可以为投资者提供决策参考。
为了验证PNEN模型的效果,我们在实际的股票市场数据上进行了实验。
结果表明,与传统神经网络模型相比,PNEN 模型在预测股票走势方面具有更好的准确性和稳定性。
基于遗传算法的地面中重频PD雷达PRF组选择
PD 为一组 PR F采用 M /N 准则的检测概率 ,则可得
PD
N
=Σ
N
( Pd ) k (1 - Pd ) (N - k)
( 1)
k =M k
式中 : N 为总的 PRF 数 目 ; M 为 检测 所需的 PRF 数 目 ; k为清晰的 PRF数目 。取 Pd = 0. 5 ,以 3 /7 准则为 例 , 图 1给出了不同 Nc 下的 PD ~Pd 关系曲线 。当单 一 PRF 上的检测概率 Pd 一定时 , 增加清晰 PRF 的数 目可以提高系统的检测性能 。
1 最优 PRF 组的选择
PRF 脉冲重复频率的选择应使得 盲区的范围减 小 ,同时还应使得目标的发现概率最大 。 1. 1 M /N准则检测概率
假设各 PRF 上的检测概率 Pd 相同且相互独立 ,
图 1 采用 M /N准则单重 PR F上 Pd 与 PD 关系曲线
1. 2 地面雷达 3 /7准则合理论证 为了减小距离和多普勒盲区 ,机载中 PRF 雷达一
【 Key word s】PD rada r; PRF set; generic a lgorithm; blind area
0 引 言
近年来 ,为进一步增强杂波抑制能力 ,地面对空情 报雷达开始采用中重 复频率脉冲多普 勒 ( PD )体制 。 中重频 PD雷达在距离和速度上都是模糊的 ,从而造 成距离和速度二维盲区 。对于大多数采用脉冲压缩体 制的地面雷达来说 , 距离盲区尤其严重 。中重频雷达 PRF 的选择受到多种因 素的限制 [ 1 ] , 如重频范围 、脉 冲宽度 、盲区大小 、幻影目标等 ,因此重频的选择是一
《2024年橙皮素早期干预对APPswe-PS1dE9双转基因小鼠Aβ生成的影响》范文
《橙皮素早期干预对APPswe-PS1dE9双转基因小鼠Aβ生成的影响》篇一橙皮素早期干预对APPswe-PS1dE9双转基因小鼠Aβ生成的影响一、引言近年来,阿尔茨海默病(AD)已成为全球范围内的公共卫生问题。
在AD的发病机制中,Aβ的异常沉积与沉积引起的神经毒性是关键因素之一。
为了探讨有效降低Aβ生成及减缓AD发展的方法,越来越多的研究开始关注天然产物的应用。
橙皮素作为一种天然的黄酮类化合物,其抗氧化、抗炎及神经保护作用备受关注。
本篇论文旨在研究橙皮素早期干预对APPswe/PS1dE9双转基因小鼠Aβ生成的影响。
二、材料与方法1. 材料橙皮素:选用纯度较高的橙皮素原料。
APPswe/PS1dE9双转基因小鼠:用于模拟AD病理过程的小鼠模型。
实验试剂与仪器:包括酶联免疫吸附试验(ELISA)试剂、显微镜、离心机等。
2. 方法(1)分组与干预:将APPswe/PS1dE9双转基因小鼠随机分为实验组和对照组,实验组自出生后即开始给予橙皮素干预,对照组则不作处理。
(2)样本收集:在干预一定时间后,收集小鼠脑组织样本。
(3)Aβ检测:采用ELISA法检测脑组织中Aβ的含量。
(4)统计分析:对实验数据进行统计分析,比较两组小鼠Aβ含量的差异。
三、实验结果1. 橙皮素对APPswe/PS1dE9双转基因小鼠体重的影响:实验期间,两组小鼠体重均呈增长趋势,但两组间体重无明显差异,说明橙皮素的干预未对小鼠体重造成影响。
2. 橙皮素对APPswe/PS1dE9双转基因小鼠脑组织Aβ含量的影响:实验组小鼠脑组织中Aβ含量较对照组显著降低(P<0.05),说明橙皮素早期干预能够显著降低Aβ的生成。
3. 统计分析结果:通过t检验分析两组小鼠Aβ含量的差异,结果显示P值小于0.05,具有统计学意义。
四、讨论本实验结果表明,橙皮素早期干预能够显著降低APPswe/PS1dE9双转基因小鼠脑组织中Aβ的生成。
这可能与橙皮素的抗氧化、抗炎及神经保护作用有关。
基因毒性杂质之结构警示(欧洲)
EUR 23844 EN -2009Development of structural alerts for the in vivo micronucleus assay inrodentsRomualdo Benigni a , Cecilia Bossa a , Olga Tcheremenskaia aand Andrew Worthb aIstituto Superiore di Sanita’, Environment and Health Department,Rome, Italy b Institute for Health & Consumer Protection, European Commission -Joint Research Centre, Ispra, ItalyThe mission of the IHCP is to provide scientific support to the development and implementation of EU policies related to health and consumer protection.The IHCP carries out research to improve the understanding of potential health risks posed by chemical, physical and biological agents from various sources to which consumers are exposed.European CommissionJoint Research CentreInstitute for Health and Consumer ProtectionContact informationAddress: TP 582E-mail: andrew.worth@ec.europa.euTel.: +39 0332 789566Fax: +39 0332 786717http://http://ecb.jrc.ec.europa.eu/qsar/http://ec.europa.eu/dgs/jrc/Legal NoticeNeither the European Commission nor any person acting on behalf of the Commission is responsible for the use which might be made of this publication.A great deal of additional information on the European Union is available on the Internet.It can be accessed through the Europa serverhttp://europa.eu/JRC 52274EUR23844 ENISSN 1018-5593Luxembourg: Office for Official Publications of the European Communities© European Communities, 2009Reproduction is authorised provided the source is acknowledgedPrinted in ItalyABSTRACTIn vivo mutagenicity and carcinogenicity studies are posing a high demand for test-related resources. Among these studies, the micronucleus test in rodents is the most widely used, as follow up to positive in vitro mutagenicity results. A recent survey of the (Q)SAR models for mutagenicity and carcinogenicity has indicated that no (Q)SAR models for in vivo micronucleus are available in the public domain. Therefore, the development and extensive use of estimation techniques such as (Q)SARs, read-across and grouping of chemicals, promises to have a huge animal saving potential for this endpoint. In this report, we describe the identification of structural alerts for the in vivo micronucleus assay, and provide the list of underlying chemical structures. These structural alerts provide a coarse-grain filter for the preliminary screening of potential in vivo mutagens.LIST OF ABBREVIATIONSEPA Environmental Protection AgencyEU European UnionFDA Food and Drug AdministrationHOMO Highest Occupied Molecular OrbitalISS Istituto Superiore di Sanita’JRC Joint Research CentreLUMO Lowest Unccupied Molecular OrbitalOECD Organisation for Economic Cooperation and Development(Q)SAR(Quantitative)Structure-Activity RelationshipREACH Registration Evaluation and Authorisation of CHemicalsROC Receiver Operating CurveSA Structural AlertSA_BB Benigni-Bossa structural alerts for mutagnicity /carcinogenicity in ToxtreeSA_Mic Structural alerts refers for the in vivo micronucleus assay inToxtreeSA_Prot Structural alerts for protein binding in the OECD QSAR ToolboxCONTENTS1.Introduction (6)2.Structural alerts (8)3.Development of structural alerts for the in vivo micronucleus assay (10)4. Final considerations (20)5.References (21)Appendix 1 (23)1.IntroductionMutagenicity testing is an important part of the regulatory hazard assessment of chemicals. It is undertaken for two main reasons: a) to detect chemicals that might cause genetic damage in germ cells, and thus increase the burden of heritable (genetic) disease in the human population; and b) to detect chemicals that might be carcinogenic (based on the assumption that mutagenesis, for example in somatic cells, is a key event in the process of carcinogenesis). Since no method is able alone to detect all possible genotoxic events, a wide array of test systems has been developed and accepted internationally in regulatory schemes.Most often, these methods are used within a 2-tiered integrated testing approach: Tier 1 includes in vivo assays, and Tier 2 includes in vivo assays. As a matter of fact, mutagenicity testing was the first toxicity endpoint for which in vivo assays were accepted for regulatory testing, some 25 years ago. The latter usually comprise bacterial mutagenicity and cytogenetics tests, although gene mutation testing in cultured mammalian cells is sometimes also undertaken.Tier 2 of the testing strategy involves the use of short-term in vivo studies (usually a bone-marrow cytogenetics assay) to assess whether any potential for genotoxicity detected at the Tier 1 in vivo stage is actually expressed in the whole animal. Thus, negative results in vivo are usually considered sufficient to indicate lack of mutagenicity, whereas a positive result is not considered sufficient to indicate that the chemical represents a mutagenic hazard (i.e. it could be a false positive). The above approach to genotoxicity testing has been adopted throughout the EU1,and has been recommended internationally as part of the strategy for predicting and quantifying mutagenic and carcinogenic hazard (Ashby et al.,1996; Combes et al.,2007; Kirkland and Speit,2008; Lilienblum et al.,2008).1http://guidance.echa.europa.eu/docs/guidance_document/information_requirements_r7a_en.p df?vers=20_08_08According to an assessment carried out by the former European Chemicals Bureau (ECB), the in vivo mutagenicity studies, shortly followed by carcinogenicity, are posing high demand for test-related recourses (Pedersen et al.,2003; Van der Jagt et al.,2004). Among those, the micronucleus test in rodents is the most widely used, as follow up to positive in vivo mutagenicity results. A recent survey of the (Q)SAR models for mutagenicity and carcinogenicity (performed jointly by ISS and the JRC) has indicated that no (Q)SAR models for in vivo micronucleus are available in the public domain (Benigni et al.,2007): therefore, the development and extensive use of estimation techniques such as (Q)SARs, read-across and grouping of chemicals, might have a huge saving potential for this endpoint.In this report, we describe: a) the collection of data on chemicals tested with the in vivo micronucleus assay; b) preliminary analyses of the data; c) the identification of Structural Alerts (SA) proper to this toxicological endpoint. First, some background information on the concept of SA is provided.2.Structural alertsThe SAs for a toxicological endpoint are molecular functional groups or substructures known to be linked to that type of toxicity.The SAs are a coarse-grained approach to the use of Structure-Activity Relationships (SAR) to understand the toxicity mechanisms and to predict the toxic activity of chemicals. Because of their nature, the SAs have the role of pointing to chemicals potentially toxic, whereas no conclusions or indications about nontoxic chemicals are possible (except by exclusion) (Benigni and Bossa,2006; Benigni and Bossa,2008).A set of chemicals characterized by the same SA constitute a family (class) of compounds that share the same mechanism of action. The reactivity of a SA can be modulated or abolished by the remaining part of the molecule in which the SA is embedded. At a coarse-grain level, such modulating effects can be represented by other molecular substructures (e.g., bulky groups ortho to an aromatic amine group) that are known to have an influence on the reactivity of the SA. Usually, the knowledge on the modulating substructures is quite limited for most of the SAs, thus it provides limited help in deciding which chemicals in a class will actually be toxic and viceversa. A powerful generalization of the Structure-Activity Relationships is provided by the Quantitative Structure-Activity Relationship (QSAR) analysis, which produces a mathematical model that links the biological activity to a limited number of physical chemical or other molecular properties (descriptors) with general relevance. Since most of the descriptors have continuous values, the QSARs provide fine-tuned models of the biological activity,and can give account of subtle differences. General introductions on QSAR are given elsewhere (Hansch and Leo, 1995, Hansch et al.,2002). Thus the SAs are not a discriminant model on the same ground of the QSAR models: the latter produce estimates for both positive and negative chemicals, as well as for the gradation of toxic potency.The main role of the SAs is that of preliminary, or large-scale screenings. They are excellent tools for coarse-grain characterization of chemicals, including: description of sets of chemicals, preliminary hazard characterization, category formation and priority setting (enrichment). Since fine-tuned QSARs do not exist for many types of chemicals, the models based on SAs hold a special place in predictive toxicology. Theknowledge on the action mechanisms as exemplified by the SAs is routinely used in SAR assessment in the regulatory context (see, for example, the mechanistically-based reasoning as presented in Woo et al. (2002). In addition, the SAs are at the basis of popular commercial (e.g., DEREK, by Lhasa Ltd.2) and non-commercial software systems (e.g., Oncologic, by US Environmental Protection Agency[EPA]3).Recently, as follow-up of the collaboration between ISS and JRC,a rulebase for mutagens and carcinogens has been designed and implemented in the software Toxtree 1.51. It uses a structure-based approach consisting of a new compilation of SAs for carcinogenicity and mutagenicity. It also offers three mechanistically based QSARs for congeneric classes (aromatic amines and aldehydes) (Benigni et al., 2008a). Toxtree 1.51 is freely available from the JRC website.42/3/oppt/newchems/tools/oncologic.htm4http://ecb.jrc.ec.europa.eu/qsar/qsar-tools/index.php?c=TOXTREE3.Development of structural alerts for the in vivomicronucleus assay3.1 DataThe compilation of SAs for the in vivo micronucleus assay in rodents provided here, is based on both the existing knowledge on the mechanisms of toxic action and a structural analysis of the chemicals tested in the assay.The in vivo micronucleus data in the public domain is quite limited. A search of the Chemical Carcinogenesis Research Information System(CCRIS) at the Toxnet website with the query: “in vivo micronucleus” points only to 240 chemicals.5For this work, the remarkably larger commercial database by Leadscope Inc., called “FDA SAR Genetox Database” was used.6This database contains more than 700 chemicals tested in in vivo micronucleus with rodents, and includes data from both the public domain and the US Food and Drug Administration (FDA) files. A large majority of data were based on the analysis of micronuclei in bone marrow cells; for details on the technique, see for example, Krishna and Hayashi (2000).3.1 Preliminary analysesSince the main role of the in vivo micronucleus assay in regulatory schemes is that of confirming (or disproving) the positive in vitro results, it is of interest to check how the in vivo micronucleus results relate to the rodent carcinogenicity data and to the primary in vitro prediction test, i.e., the Salmonella typhimurium(Ames) test.Tables I and II display the relationships between the in vivo micronucleus ad the two reference tests. The results for rodent carcinogenicity and the Ames test were retrieved from the freely available ISSCAN v3a database,7which is characterized by:5/cgi-bin/sis/search6/product_info.php?products_id=777http://www.iss.it/ampp/dati/cont.php?id=233&lang=1&tipo=7a) the high quality of both chemical and biological information; b) the QSAR-ready format (Benigni et al.,2008b). Obviously, the total numbers of chemicals in the two tables are relative only to those chemicals tested in both systems.Table I. Contingency table comparing the results of the rodent carcinogenicity testwith the micronucleus testTable II:Contingency table comparing the results of the Salmonella typhimuriumassay with the micronucleus testTable I shows that is the in vivo micronucleus assay is poorly sensitive to the rodent carcinogens: about 60% of the rodent carcinogens are not detected by the micronucleus. The poor sensitivity of the micronucleus assay to potential genotoxins is also apparent from Table II.It should be emphasized that the present results obtained with the large Leadscope micronucleus database are in agreement with previous analyses based on smaller datasets in the public domain (Benigni,1995).In a second round of analyses, the extent to which the micronucleus data are related to well established indicators of DNA and protein binding was checked. This in view of the plethora of the reported mechanisms of micronucleus induction. As a matter of fact, micronuclei are markers of both aneugenic (change in the chromosomes number, usually by loss) and clastogenic (chromosome breakage) effects. It is generally assumed that such effects are generated through a range of different pathways. Evidence (mainly gathered from in vitro studies) indicates that micronuclei can be induced e.g., by typical DNA-attacking agents (e.g., alkylating agents like methylmethane sulfonate), by mitotic spindle poisons (e.g., colcemide, vincristine), or by inhibitors of cytokinesis (e.g., cytochalasin B). The latter effects are probably due to interference with proteins. Other chemicals are thought to be clastogenic through aspecific disturbance of cytokinesis due to lipophilicity (Dorn et al.,2007).The relative influence of DNA and protein binding on micronucleus generation was checked by recording the distribution of structural alerts for the two effects in the Leadscope in vivo micronucleus database. As probes for DNA binding, we used the structural alerts for carcinogenicity / mutagenicity implemented in Toxtree 1.51. As a matter of fact, the large majority of these alerts refer to genotoxic carcinogenicity, which is assumed to be caused through direct interaction with DNA (Benigni and Bossa, 2008). As probes for protein binding, we used the alerts implemented in the Organisation for Economic Cooperation and Development (OECD) QSAR Toolbox.8 These alerts were mainly developed from the mechanistic knowledge on skin sensitization, and model the covalent binding to proteins.The results of the above analysis is displayed in Figure 1 as a ROC graph. It appears that the structural alerts for carcinogenicity /mutagenicity correlate to some extent with the induction of micronuclei, whereas those for protein covalent binding show no correlation (in the graph, they are on the diagonal line which represents random results).8/document/23/0,3343,en_2649_34379_33957015_1_1_1_1,00.htmlFigure 1. Receiver Operating Curve showing the concordance of two sets of structural alerts with the results of the in vivo micronucleus assay(SA_BB refers to the Benigni-Bossa alerts in Toxtree; SA_Prot refers to the alerts for proteinbinding in the OECD QSAR Toolbox)3.3 Structural Alerts for in vivo micronucleus assaySince the above analyses pointed to genotoxic effects as an important determinant of micronuclei induction, we developed the list of Structural alerts for in vivo micronucleus using the carcinogenicity / mutagenicity alerts in Toxtree as a core , and then searching for additional substructures specific to the micronucleus-positive chemicals. From the Toxtree alerts for carcinogenicity / mutagenicity,we excluded four alerts specific for non-genotoxic mechanisms of carcinogenicity.Using linear discriminant analysis as an analytical tool and ROC plots as a graphical tool, a series of additional substructures were added / removed to / from the Toxtreealerts in order to increase sensitivity and specificity.In these exploratory analyses, wescreened the very large collection of substructural patterns and functional groups (more than 27,000) contained in the software Leadscope Enteprise 2.4.15-6. We also re-checked the Toolbox protein binding alerts for individual substructures related with micronucleus induction.The result is the optimized list of alerts in Appendix 1. Together with the Toxtree alerts, it contains five additional substructures identified in the course of this research. For the sake of clarity,the codes of the alerts in Toxtree are maintained, whereas the five additional alerts have new codes.Figure 2 displays the agreement between the alerts for in vivo micronucleus, and the experimental results for this endpoint. Out of 547 negatives, the specificity of the SAs is 0.57. The sensitivity is 0.65 out of 182 positives. The overall accuracy is 0.59. For a comparison, the ROC graph shows the newly developed alerts for micronucleus together with those for DNA and protein binding. It appears that the performance of the final list of alerts is considerably higher than that of the DNA binding and Protein binding alerts.Table III gives the true positive rate for the individual alerts.Figure 2 Receiver Operating Curve showing the concordance of structural alerts for the in vivo micronucleus assay with the experiemtnal results for this assay(SA_Mic refers to the in vivo micronucleus alerts in Toxtree)Table III: Characterisation of Structural Alerts.3.4 Further analyses on the alerts for micronucleusA striking evidence in Table III is the relatively low percentage of true positives identified by many SAs. In other words, often the toxic potential of the alerts is not translated into actual toxicity in the experimental system. For a comparison, the True Positive Rate of the various alerts for mutagenicity /carcinogenicity in Toxtree is remarkably higher, ranging from 70 to 100% (Benigni and Bossa,2008).The above result contributes to better understand the evidence in Tables I and II, where it appears that the micronucleus assay has many more negatives than the carcinogenicity bioassay and the Salmonella mutagenicity test. Table III indicates that the low sensitivity of the micronucleus assay is largely due to the fact that often,chemical functionalities and substructures which are supposed to be reactive do not exert their potential reactivity in this experimental system.The issue of the low sensitivity of the micronucleus assay has been recognized by scientists involved in research aimed at improving the available short-term mutagenicity assays; as a matter of fact, validation of further, more sensitive in vivo assays (e.g., in vivo Comet assay) is presently in progress (Kirkland and Speit,2008).In the context of this research, we investigated if a general effect of bioavailability on the limited sensitivity of micronucleus was apparent. To this aim, we considered two chemical descriptors well known as to be linked to bioavailability: logP (hydrophobicity) and Molar Refractivity (MR) (Hansch and Leo,1995). The two descriptors were calculated with the C-QSAR software (Daylight, Inc.)9for all the chemicals in the micronucleus database. For the two parameters, Table IV reports the ranges of values for positive and negative micronucleus results.Table IV: Ranges of C-logP and C-MR in chemicals assayed withthe micronucleus testIt appears that the micronucleus positives cover a more limited range of logP values than the micronucleus negatives; however, the consideration of exclusion values for logP in combination with the SAs did not improve the overall performance (results not shown).Whereas no general effect of logP (or MR) was found, analyses on the individual chemical classes showed that logP cut-offs can be identified for the classes of Nitroaromatics (Negatives at logP > 0.0), Aromatic Diazo (Negatives at logP < 3.7),9/about/index.htmland Oxolanes (Negatives at logP > 1.5). The consideration of these cut-offs increases the specificity of the SAs from0.57 to 0.60.The above result suggests a possible strategy to understand and modeling the many negative results observed with the micronucleus. Since the bone marrow (main target of the test) is an organ easily accessible by the blood stream, it can be hypothesized that the lack of effect shown by several chemicals with SAs (hence potentially reactive) is due to the many possible targets for reaction encountered in the in vivo situation; this diminishes the probability for the chemicals of reaching, and interacting with the molecular target(s) of the micronucleus test. For example, highly reactive chemicals will probably react with any target encountered in their way (e.g., proteins, water)before reaching the bone marrow. Thus it can be envisaged that QSARs for individual chemical classes should be developed, and that they should consider parameters linked to chemical reactivity(such as HOMO and LUMO energies). It can be hypothesized that the models derived from these QSARs will contribute to modulate the individual SAs.4. Final considerationsStructural alerts point to classes of chemicals with the potential to cause toxic effects (here, in vivo micronucleus). Since this potential is modulated in each molecule by the rest of the structure (e.g., other functional groups, electronic structure, bulky groups), not all chemicals in a class are equally toxic. In the case of the SAs identified in the present study for the in vivo micronucleus test, the percentage of chemicals that have SAs but are not active in the test system is particularly high. This evidence agrees with, and rationalizes the notion that this test system is sensitive to genotoxins to a limited extent, and does not respond to a large number of recognized carcinogens and mutagens. For this reason, a positive in vivo micronucleus result adds a strong weight to an in vivo positive mutagenicity result, whereas a negative in vivo micronucleus result has a much lower relevance. The availability of a wider range of in vivo mutagenicity assays is a priority for the present regulatory strategies.Within the above perspective, the SAs identified in this study provide a coarse-grain filter for a preliminary screening of potentially in vivo mutagens. In a risk assessment process, further information(e.g., QSARs for individual classes, experiments) is necessary to complete this initial screening step.5.ReferencesAshby,J., M.D.Waters, J.Preston, I.D.Adler, G.R.Douglas, R.Fielder, M.D.Shelby,D.Anderson, T.Sofuni, H.N.B.Gopalan, G.Becking and C.Sonich-Mullin(1996). IPCS harmonization of methods for the prediction and quantification of human carcinogenic/mutagenic hazard, and for indicating the probable mechanism of action of carcinogens. Mutat.Res./Fundamental and Molecular Mechanisms of Mutagenesis. 352:153-157.Benigni,R. (1995). Mouse bone marrow micronucleus assay: relationships with in Vitro mutagenicity and rodent carcinogenicity. J.Toxicol.Environ.Health.45:337-347.Benigni,R. and C.Bossa(2006). Structural alerts of mutagens and carcinogens.put.-Aid.Drug Des.2:169-176.Benigni,R. and C.Bossa(2008). Structure Alerts for carcinogenicity,and the Salmonella assay system: a novel insight through the chemical relational databases technology. Mutat.Res.Revs.659:248-261.Benigni,R., C.Bossa, N.G.Jeliazkova, zeva and A.P.Worth(2008a). The Benigni / Bossa rulebase for mutagenicity and carcinogenicity -a module of Toxtree. JRC Report EUR 23241 EN. European Commission Joint Research Centre, Ispra, Italy.http://ecb.jrc.ec.europa.eu/DOCUMENTS/QSAR/EUR_23241_EN.pdfBenigni,R., C.Bossa, A.M.Richard, and C.Yang (2008b). A novel approach: chemical relational databases, and the role of the ISSCAN database on assessing chemical carcinogenicity.Ann.Ist.Super.Sanità. 44:48-56.Benigni,R., C.Bossa, zeva and A.P.Worth(2007).Collection and evaluation of (Q)SAR models for mutagenicity and carcinogenicity. JRC Report EUR 22772 EN. European Commission Joint Research Centre, Ispra, Italy.http://ecb.jrc.ec.europa.eu /documents/QSAR/EUR_22772_EN.pdfCombes,R., C.Grindon, M.T.D.Cronin, D.W.Roberts and J.Garrod(2007). Proposed integrated decision-tree testing strategies for mutagenicity and carcinogenicity in relation to the EU REACH legislation.ATLA. 35:267-287.Dorn,S.B., G.H.Degen, T.Müller, D.Bonacker, H.F.P.Joosten, J.van der Louw,F.A.A.van Acker and H.M.Bolt(2007). Proposed criteria for specific and non-specific chromosomal genotoxicity based on hydrophobic interactions.Mutat.Res./Genetic Toxicology and Environmental Mutagenesis. 628:67-75. Hansch,C., D.Hoekman, A.Leo, D.Weininger and C.D.Selassie(2002).Chem-bioinformatics: comparative QSAR at the interface between chemistry and biology.Chem.Revs.102:783-812.Hansch,C. and A.Leo (1995). Exploring QSAR. 1. Fundamentals and applications in chemistry and biology.American Chemical Society. Washington, D.C.Kirkland,D. and G.Speit(2008). Evaluation of the ability of a battery of three in vitro genotoxicity tests to discriminate rodent carcinogens and non-carcinogens III.Appropriate follow-up testing in vivo.Mutat.Res.654:114-132.Krishna,G. and M.Hayashi(2000). In vivo rodent micronucleus assay: protocol, conduct and data interpretation.Mutat.Res.455:155-166.Lilienblum,W., W.Dekant, H.Foth, T.Gebel, J.G.Hengstler, R.Kahl, P.J.Kramer,H.Schweinfurth and K.M.Wollin(2008). Alternative methods to safety studiesin experimental animals: role in the risk assessment of chemicals under the new European Chemicals Legislation (REACH).Regulat.Toxicol.82:211-236.Pedersen,F., J.de Brujin, S.J.Munn and K.Van Leeuwen(2003).Assessment of additional testing needs under REACH. Effects of (Q)SARs, risk based testing and voluntary industry initiatives. JRC report EUR 20863 EN. European Commission Joint Research Centre, Ispra, Italy.http://ecb.jrc.ec.europa.eu/home.php?CONTENU=/DOCUMENTS/REACH/PUBLICATIONS/ Van der Jagt,K., S.J.Munn, J.Torslov and J.de Brujin (2004).Alternative approaches can reduce the use of test animals under REACH. Addendum to the Report "Assessment of addtional testing needs under REACH. Effects of (Q)SARs, risk based testing and voluntary industry initiatives. JRC Report EUR 21405 EN.European Commission Joint Research Centre, Ispra, Italy.http://ecb.jrc.ec.europa.eu/home.php?CONTENU=/DOCUMENTS/REACH/PUBLICATIONS/ Woo,Y.T., i, J.L.McLain, M.Ko Manibusan and V.Dellarco(2002). Use of mechanism-based structure-activity relationships analysis in carcinogenic potential ranking for drinking water disinfection by-products. Environ.Health Perspect.110:75-87.Appendix 1European CommissionEUR 23844 EN–Joint Research Centre –Institute for Health and Consumer ProtectionTitle: Development of Structural alerts for the in vivo micronucleus assay in rodentsAuthor(s): Benigni R, Bossa C, Tcheremenskaia O and Worth A Luxembourg: Office for Official Publications of the European Communities 2009–42pp. –21x 29.7cmEUR –Scientific and Technical Research series –ISSN 1018-5593AbstractIn vivo mutagenicity and carcinogenicity studies are posing a high demand for test-related resources. Among these studies, the micronucleus test in rodents is the most widely used, as follow up to positive in vitro mutagenicity results. A recent survey of the (Q)SAR models for mutagenicity and carcinogenicity has indicated that no (Q)SAR models for in vivo micronucleus are available in the public domain. Therefore, the development and extensive use of estimation techniques such as (Q)SARs, read-across and grouping of chemicals, promises to have a huge animal saving potential for this endpoint. In this report, we describe the identification of structural alerts for the in vivo micronucleus assay, and provide the list of underlying chemical structures. These structural alerts provide a coarse-grain filter for the preliminary screening of potential in vivo mutagens.。
211132683_东南亚十二节段RNA病毒荧光定量qRT-PCR和常规RT-PCR检测方法的建立
·研究论文·Chinese Journal of Animal Infectious Diseases中国动物传染病学报摘 要:东南亚十二节段RNA 病毒属为呼肠孤病毒科中的一个新属,该属包括版纳病毒(BAV )、芒市病毒(MSV )、卡皮罗病毒(KDV )和辽宁病毒(LNV )四种病毒。
为建立上述四种病毒的群特异性核酸检测方法,本研究根据BAV 、MSV 、KDV 和LNV 毒株VP12基因序列的保守区,设计特异性引物和TaqMan 探针,建立了荧光定量qRT-PCR 和常规RT-PCR 检测方法,并分别进行了特异性、灵敏性和重复性的检测。
实验结果表明,两种检测方法分别对四种病毒均有特异性的扩增和荧光信号检出,而对流行性出血病病毒(EHDV )、蓝舌病病毒(BTV )、中山病病毒(CHUV )、广西环状病毒(GXOV )、阿卡斑病毒(AKAV )、云南环状病毒(YUOV )等病毒无扩增和无有效荧光信号检出,具有较好的特异性。
灵敏性实验结果显示,荧光定量qRT-PCR 检测四种病毒核酸的下限可达10 copies/μL ,常规RT-PCR 的检测下限为102 copies/μL ,荧光定量方法灵敏度是常规方法的10倍。
四种病毒荧光定量qRT-PCR 方法的组内和组间重复试验标准差均小于0.8,变异系数均小于3%,表明该方法均具有良好的稳定性。
以上结果表明,本研究建立的BAV 、MSV 、KDV 和LNV 四种病毒的荧光定量qRT-PCR 和常规RT-PCR 方法具有快速、准确、稳定等优点,可为四种病毒的早期诊断、快速检测、流行病学调查和实验研究等提供有效的技术方法。
关键词:版纳病毒;芒市病毒;卡地皮诺病毒;辽宁病毒;RT-PCR ;病毒检测中图分类号:S852.65文献标志码:A文章编号:1674-6422(2023)01-0098-08Development of Real-time Fluorescent Quantitative RT-PCR and ConventionalRT-PCR for Detection of Southeastern Asian Dodeca RNA Viruses收稿日期:2020-09-28基金项目:国家重点研发计划(2017YFC1200505);国家公益性行业(农业)科研专项(201303035);云南省中青年学术和技术带头人后备人才培养项目(2017HB055)作者简介:杨振兴,男,硕士,副研究员,主要从事牛羊虫媒病毒研究;李占鸿,男,硕士,助理研究员,主要从事牛羊虫媒病毒研究通信作者:廖德芳,E-mail:*******************;杨恒,E-mail:*************************东南亚十二节段RNA 病毒荧光定量qRT-PCR 和常规RT-PCR 检测方法的建立杨振兴,李占鸿,李卓然,李华春,廖德芳,杨 恒(云南省畜牧兽医科学院 热带亚热带动物病毒病重点实验室,昆明650224)2023,31(1):98-105Abstract: Southeastern Asian dodeca RNA viruses (Seadornavirus ) belong to a new genus of the Reoviridae family, which includes Banna virus (BA V), Mangshi virus (MSV), Kadipiro Virus (KDV) and Liaoning virus (LNV). In order to develop a group-specifi c nucleic acid detection method for BA V , MSV , KDV and LNV , specifi c primers and TaqMan probes were designed based on the conserved regions of the VP12 gene sequences of the 4 viruses for development of a real-time quantitative RT-PCR (qRT-PCR) and conventional RT-PCR. The results showed that both assays specifi cally amplifi ed the 4 viruses b u t not for epizootic hemorrhagic disease virus (EHDV), bluetongue Virus (BTV), Chuzan virus (CHUV), Guangxi Orbivirus (GXOV), Akabane virus (AKA V) and Yunnan Orbivirus (YUOV). The detectionYANG Zhenxing, LI Zhanhong, LI Zhuoran, LI Huachun, LIAO Defang, YANG Heng(Yunnan Tropical and Subtropical Animal Virus Disease Laboratory, Yunnan V eterinary and Animal Science Institute, Kunming 650224, China)· 99 ·杨振兴等:东南亚十二节段RNA 病毒荧光定量qRT-PCR 和常规RT-PCR 检测方法的建立第31卷第1期东南亚十二节段RNA病毒属(Seadornavirus )隶属呼肠孤病毒科(Reoviridae),为2005年国际病毒分类委员会(International Committee for the Taxonomy of Viruses, ICTV)第八次会议新确认的一个病毒属[1]。
基于2b-RAD测序的四倍体马铃薯熟性相关的分子标记开发
DOI: 10.3724/SP.J.1006.2022.14138基于2b-RAD测序的四倍体马铃薯熟性相关的分子标记开发惠志明徐建飞简银巧卞春松段绍光胡军李广存金黎平*中国农业科学院蔬菜花卉研究所/ 农业农村部薯类作物生物学和遗传育种重点实验室,北京100081摘要:马铃薯熟性是由多基因控制的数量性状,是我国不同栽培区划选择适宜品种的重要指标之一。
本研究以晚熟品种中薯18号和早熟品种中薯5号及其F1分离群体为材料,2018—2019年连续2年对“中薯18号(母本) ×中薯5号(父本)”杂交分离群体进行熟性评价,从中筛选出极端晚熟和极端早熟的基因型各30个,并分别构建极端晚熟和极端早熟基因组DNA 混池。
利用简化基因组2b-RAD (2b-restriction site-associated DNA)技术测序,寻找差异标签开发出3个与熟性连锁的分子标记SCARA2-2、SCARA4-21和SCARA5-16,3个分子标记联合使用对熟性分离群体子代进行验证,晚、早熟表型符合率分别到达了87.5%和93.0%,这些分子标记的开发和联合使用对辅助马铃薯熟性选择具有重要的参考价值。
关键词:马铃薯;熟性;2b-RAD;分子标记2b-RAD based maturity associated molecular marker identification in tetraploid potato (Solanum tuberosum L.)HUI Zhi-Ming, XU Jian-Fei, JIAN Yin-Qiao, BIAN Chun-Song, DUAN Shao-Guang, HU Jun, LI Guang-Cun, and JIN Li-Ping*Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaAbstract: Potato maturity is a quantitative trait controlled by multiple genes and it is one of the major agronomic characteristics for selecting suitable varieties in different agro-ecological zones. In this study, the maturity of the segregating population derived from Zhongshu 18 (♀) ×Zhongshu 5 (♂) was evaluated in 2018and 2019. Respectively, 30 offsprings with extremely late or early maturity were selected and used to construct early and late maturing genomic pool. Three molecular markers (SCARA2-2, SCARA4-21, and SCARA5-16) linked to maturity were identified by simplified genome 2b-RAD (2b-restriction site-associated DNA) sequencing. The maturity phenotypic association rate of the three-marker-combination for the late and early maturity genotype verification reached 87.5% and 93.0%, respectively. Thus, these molecular markers are valuable for markers assisted selection in potato maturity breeding.Keywords: potato; maturity; 2b-RAD; molecular markers马铃薯(Solanum tuberosum L.)是世界第三大粮食作物,在我国各个生态区域都有广泛种植,为缓解我国粮食安全压力和振兴西部边远山区经济建设具有重要的战略意义[1]。
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Development of a PC BasedGeneric Radar Display SimulatorCommander Ian Curl, RAN and Mr. Andrew Weisz, Serco Australia Pty LtdRoyal Australian Navy Surface Warfare SchoolHMAS WATSONSUMMARYSurface Warfare training for Royal Australian Navy (RAN) personnel is undertaken at HMAS WATSON in Sydney. Individual operator, command team and task group level tactical training is conducted on a variety of systems, including functional simulators, Milspec equipment and CBT suites. Most of the smaller training systems have been developed at WATSON by personnel from the RAN and Serco Australia Pty Ltd, the major on-site engineering support contractor. Even though these training systems have been developed and built in-house at very low cost, they have proven to be extremely effective for shore based operator training.This paper discusses the development of a medium fidelity PC based Generic Radar Display Simulator that has been designed to provide shore based radar operator instruction for training functions that previously could only be carried out at sea. The simulator was developed to run on a ‘standard’ PC, and incorporates an emulated UPA-59 IFF (Identify Friend or Foe) panel. The simulator provides the ability to generate emulated radar displays incorporating features such as sea clutter, atmospheric interference, coast lines, hostile jamming, mutual interference as well as surface and air contacts.The performance and fidelity achieved on a PC with SVGA display adapter, the design approach taken and the various development tools utilised are described. The impact of the new RAD (Rapid Application Development) tools like Delphi is also discussed.INTRODUCTION1.1 Initial User RequirementIn January 1995, the Combat Systems (CS) faculty at HMAS WATSON raised the requirement for a training tool that would facilitate the introduction of novice radar operators to the display unit and controls of typical radars in use by the RAN. Based on this request, WATSON’s on-site Systems Engineering contractor looked at the feasibility of developing a low to medium fidelity Radar Display Simulator. As only introductory training was involved, fidelity issues were considered relatively unimportant. However, with both the RAN and the RAAF having very limited access to active jamming equipment, the ability to simulate the effects of various types of hostile jamming was identified as one of the major priorities.The incorporation of an emulated UPA-59 IFF (Identify Friend or Foe) Panel was mentioned in passing as a possible future enhancement, if a satisfactory simulator could be developed.As WATSON already had a CBT (Computer Based Training) classroom with ten PC’s available, part of the requirement was to run the simulator on standard PC’s. Furthermore, the CS faculty was encouraged to consider a PC based simulator as a result of a number of PC based simulators developed earlier at WATSON, as described in a SimTecT-96 paper [1] by the authors.1.2 The Developers ReactionEven though the on-site development team had been very enthusiastic supporters of the PC platform up to this time, the immediate reaction was that the PC had been ‘over-sold’. Even though the suitability of PC’s for training systems has been pointed out by various authors, including Sweet [2] and Moriarity [3], t he consensus of opinion was that the standard PC of the time, namely a 486DX-66 running DOS would not be capable of supporting an acceptable radar simulator. However, in early 1995 two fortuitous events were to change this attitude.Firstly, while up till the end of 1994, all PC development work at WATSON had been undertaken for DOS, with the expectation of being able to by-pass 16 bit Windows by changing over to Windows-95, the constant delays to Windows-95, was making it imperative to make the change to Windows 3.1. So 16 bit Windows development had to be tackled. The Radar Display Simulator project was considered a good project to begin with. If an acceptable simulator could be developed, anything else in the future would be a ‘breeze’, and if the project failed; well, everyone had been warned that it couldn’t be done.The second event that influenced the project, was a chance encounter with an American developed DOS based Radar Display Simulator. While this particular package left a lot to be desired, it did provide the inspiration required to start WATSON’s development work.1.3 Initial Investigation of the PlatformWith the recently acquired conviction that the project was feasible, a study of the minimum hardware platform required was undertaken.It was very quickly determined that acceptable fidelity would require as a minimum, SVGA resolution (800x 600) with 256 colour palette depth. Minimum memory required was thought to be 8MB, thus the required platform emerged as a 8MB 486DX-66, with a 256 colour SVGA adapter. This configuration was not too far removed from the ‘standard’ PC of the day.STAGE 1 OF THE PROJECT2.1 Detailed User RequirementThe development proper began with detailed discussions with the subject matter experts, the radar instructors. The requirements for a useful training tools were identified as....• Low fidelity reproduction of a radar display • Provision of controls for On/Off, Video Intensity, Sweep Intensity, Focus, Range Ring Intensity, Range Selection (2 to 512 nautical miles), Range Gate On/Off, Range Gate Size, Ball Tab (cursor) On/Off & Position Control• Selectable Aerial Rotation (6 & 12 r.p.m.)• Selectable Beam Width (2 & 11 dgs)• Sector Blanking (inside & outside sector)• FTC & STC filtering• Air & Surface Contacts (with movement)• Sea Clutter and Atmospheric Interference• Coast Lines (with own ship movement)• Various types of Active Hostile Jamming• Effects of Mutual Interference from other radars• Display of Identification Friend or Foe (IFF )• Provision of ‘Out the Window’ visualsFigure 1.The OJ-194 Multi Function Display ConsoleFigure 1. shows a typical command and control console with radar display used by the RAN. The IFF control panel may be seen adjacent and to the right of the console.2.2 Design ApproachDetailed software design began with a crash course in Radar Displays for the developers. The instructors explained basic radar operation, demonstrated a typical radar display, the OJ-194 as fitted to the DDG’s and FFG’s, and passed on several ancient Royal Navy radar training videos produced in the fifties.With this new found knowledge about radars, a project Impact Analysis was undertaken, which indicated that most of the facilities detailed by the user requirements would be achievable, with the exception of the Focus Control and the provision of ‘Out the Window’ visuals. These two requirements were immediately identified as being beyond the scope of WATSON’s limited resources. However the remainder were considered not too demanding to implement.2.3 The ImplementationThe development tool chosen was Microsoft’s Visual C/C++, and the preferred approach was quickly determined as a combination of pre-computed meta-files for the selected image, and palette animation for the sweep rotation and the simulation of phosphor decay. This approach turned out to be virtually identical to that taken by Sawler & Mielke [4] in their development of a low cost PC based Simulated Ground-Based Radar System.The computation of the meta-file data was found to require around 10 seconds of processing on a 486DX-66, but after the initial computation, simulation of the sweep could be achieved with practically zero processor load by the use of palette animation.Even though a circular sweep was being simulated, surprisingly it was found to be unnecessary to use anything but small straight lines, albeit, a lot of small straight lines.2.4 Palette AnimationPalette animation in the context of the Radar Display Simulator, refers to the technique of splitting the display into 72 sectors representing 5 degree sections, and allocating different colour codes (of the 256 available) to the various lines within each section. Animation of the display then consists simply of varying the actual colour (in reality the shade of colour, as the display is monochrome), as the simulated sweep makes it’s way around the display. The intensity controls are simply implemented by varying the ‘colour’ corresponding to each code. As only the 256 entry colour palette needs to be updated for each movement of the sweep, it can be achieved with very little processor load. 2.5 Functionality AchievedOf the original functionality promised, only one item was found to be too difficult to implement. Because of the approach chosen, with all of the image computation being performed when the Radar Display is turned ‘ON’, it became impossible to allow Own-Ship motion relative to coast lines and other major portions of the image (like clouds). As the image computation was taking 10 seconds on the chosen platform, it was out of the question to perform the computation ‘on-line’ with the emulated radar display operating. Other minor deficiencies included having to re-compute the image whenever the range was changed.The major shortcoming was the somewhat large amount of memory required (12MB, 8MB for the application and 4MB for Windows).2.6 Fidelity AchievedWhile the functionality achieved was simple to quantify, questions of fidelity are much more difficult to measure. Furthermore, evaluations of fidelity tend to become subjective, with some people considering a given level of fidelity relatively low, while others might consider the same fidelity medium or even relatively high. Suffice to say that the users were more than satisfied with the fidelity achieved, and while it does not reproduce the fidelity of military radar displays, it is undoubtably a useful training tool. Some idea of the fidelity achieved may be gained from the greyscale image shown below.Figure 2.The Emulated Radar ImageThis partial screen capture shows the emulation of the sweep, with the range rings, a coast line, clouds and some jamming towards the bottom of the screen.2.7 Implementation EffortThe simulator was implemented with approximately 4 man-months of effort, and stage one was completed by the middle of 1995. Emboldened by the ease with which implementation of the Radar Display Simulator had been achieved, it was decided to extend the project to Stage 2, and evaluate the requirements for the UPA-59 Panel IFF emulation.STAGE 2 OF THE PROJECT3.1 The IFF PanelThe UPA-59 panel is a small (6 inch by 12 inch) but complex device. The panel is shown in figure 3. It consists of....• 16 two & three position toggle switches• 14 push buttons• 14 single digit mechanical displays• 5 indicator lamps (with engraving)• 4 five digit numeric LED display unit (not shown) • Complex engravings on the panelFigure 3.The UPA-59 IFF Control PanelWhile several possible approaches to the emulation of this panel were being considered, an event occurred which has since turned out to be one of the major advances regarding Windows programming. Borland released a product that took an entirely new approach to Windows software development. That product was Delphi, a visual software development tool based on Borland’s object orientated version of Pascal.3.2 Rapid Application Development (RAD) ToolsDelphi became available just as the preferred solution for the emulation of the panel was emerging as the animation of a scanned image. As Delphi immediately caught the imagination as the most powerful development tool encountered, and the animation of the switches using traditional C/C++ tools was quite daunting, it was decided to try Delphi by prototyping possible approaches to the emulation. Thus by getting a ‘feel’ for what would be required for the emulation, an estimated time for the implementation could hopefully be arrived at.To provide the scanned image, a photograph of an actual panel was taken in the Milspec FFG Ops Room at WATSON. The animation of the toggle switches was undertaken as the first attempt at using Delphi. The power of Delphi exceeded our wildest expectations, and instead of providing an estimate of the effort that would be required for the implementation, stage 2 of the project was completed entirely in a single week.The animation of the switches on the panel was implemented in two days, and only three days were required for the logical interconnection of the emulated IFF panel with the emulated Radar Display.3.3 Other RAD PackagesIt should also be noted that Delphi is not the only RAD tool available for Windows. There are similar packages like Visual Basic, Protogen, Power Builder, Authorware, IconAuthor and the latest offering, Optima++ from Powersoft. At the time of writing Optima++ was not yet available in Australia, but all the others have been evaluated and we have found Delphi vastly superior for the types of projects undertaken.3.4 Problems with Stage 2Even though stage 2 of the project was a great success, there was one significant problem. Up to this time, it was confidently thought that a 256 colour video adapter would be adequate for the complete simulator. It had already been established that 256 colours were sufficient for the Radar Display emulation, and during the development of the IFF panel emulation, 256 colours were also sufficient. Unfortunately, the two emulations required different 256 colours, so to handle both simultaneously, a video adapter with 65,000 colour depth was essential.By the end of 1995, with the ever increasing power and video resolution available for PC’s, the final platform requirements were not that outrageous. However it did mean an upgrade to the 10 PC’s in the CBT classroom, but the utility of the completed simulator was sufficient justification for the cost involved.FUTURE ENHANCEMENTS4.1 Improved PerformanceIn the first quarter of 1996, the development PC’s used at WATSON were upgraded from 486DX-66’s to Pentium-90’s with accelerated video cards. Executing the simulator on these new machines had the effect of reducing the image computation and initial display time from the earlier 10 seconds to around 3 seconds. This immediately raised the possibility of achieving the original requirement of allowing own-ship motion. It is felt that once sub one second computation time has been achieved, ‘real-time’ image computation will become feasible. Only minor additional processing power, together with minor optimisation of the software, should enable this goal to be achieved shortly.4.2 Networking with Other SimulatorsAs a ‘proof of concept’, several demonstrations have already been held at WATSON, with the Radar Display Simulator networked with some of the PC based trainers, like the AN/SLQ-32 Electronic Warfare Countermeasures simulator. If the networking were to be implemented using the DIS (Distributed Interactive Simulation) Protocol, rather than proprietary protocols it would be possible to use very low cost PC’s as additional radar displays attached to the major Command Team Trainers at WATSON. The ANZAC and FFG/DDG Trainers are to be linked in 1998 using DIS.LESSONS LEARNED5.1 Constant Increase in PC Computation PowerEven though we thought we were well aware of the ever increasing computational power of PC’s, the pace of the increase caught us by surprise. Our experience with the development of the Radar Display Simulator demonstrated yet again, that even if you feel that PC’s are not powerful enough for a given application, develop the software anyway. By the time the software is complete, chances are PC’s will provide the required power.5.2 You Never Know Until You TryAs has been the case on several occasions in the past, the major hurdle to be overcome with the project was to be convinced that it was possible to achieve. Once that conviction had been arrived at, a way was found to solve the problem. In the case of the Radar Display Simulation project, that conviction was supplied by a totally unacceptable implementation, but it was enough to convince us that a solution was possible.5.3 Never be Afraid to Change to New ToolsEven though we had been using C/C++ development tools for five years at WATSON, the benefits of the new Delphi were compelling. Naturally enough, there was some resistance on the part of some individuals to the change, based on the argument that we would be loosing all the experience gained over the past years. This is a false argument, as any new tool that requires this decision to be faced will by necessity be of compelling superiority. It only took several weeks for the sceptics to concede the advantages of the new tools, and wholeheartedly embrace the new approach to software development.We are finding that using Delphi, implementation time has been typically reduced by half, and sometimes by a significantly greater percentage.5.4 Concentrate on the Actual Training NeedThe Radar Display Simulator project has reinforced the lessons from all earlier development projects, namely the importance of concentrating on the training requirement. In the case of the Radar Display Simulator, the operative word was display. We were not trying to simulate a radar set, that was not the training requirement. Thus we did not have to concern ourselves with issues such as detection range, radar horizon, the effect of atmospheric conditions on the radar beam etc. We only produced an emulated radar display, showing the typical sorts of images that will be produced, if the given items are detectable.5.5 Use Rapid Prototyping and DemonstrateAs for all other development projects undertaken at WATSON, rapid prototyping was used for the software development, with constant demonstrations to the subject matter experts. Verbal or written descriptions of the proposed system were minimal, with system design heavily based on user feedback. Only through demonstration will the two way communication between the subject matter experts and the developers become a reality, and only with that will the minimum effort solution to meet the training needs be achievable.To make the Rapid Prototyping approach a practical proposition for Windows, it is essential to use RAD tools like Delphi. This enables ‘proof of concept’ implementations to be achieved in hours, or sometimes even minutes. Thus several alternatives may beprototyped for consideration by the subject matter experts.CONCLUSIONS6.1 The Power of the PCWe have found that by the middle of 1995, the processing power and display resolution of the ‘standard’ PC’ had reached the stage where relatively complex real-time simulations like a Radar Display Simulator were easily achievable.While initially Windows programming was quiet demanding due to the high housekeeping overheads and the phenomenal complexity of the API, recent progress with development tools like Delphi has changed the situation dramatically. With these new tools and the coming of age of 32 bit Windows (both Windows-95 and Windows-NT), software development for Windows has become simple and extremely rapid. It is conceded that Windows-NT has been around for quite a while, but the earlier lack of 32 bit development tools and it’s large memory requirements (we find 32MB a minimum) has only recently made NT suitable for mainstream applications.6.2 RecommendationsBased on all of our development activities at HMAS WATSON, and the Radar Display Simulator project in particular, we would recommend the consideration of the PC platform for all training applications. In particular, we very strongly recommend that Delphi under Windows-NT be evaluated for all projects involving the development of training systems, especially for relatively small projects of the type undertaken at HMAS WATSON.ABOUT THE AUTHORS7.1 Cmdr Ian Curl, RANCommander Ian Curl is the Deputy Director of Systems Support and Development, HMAS WATSON. He is a Weapons Electrical Engineering specialist in the Royal Australian Navy and holds a degree in Electronic Engineering from the University of South Australia. During his twenty years service with the RAN, Commander Curl has served in a variety of Combat Systems engineering positions, both at sea and ashore. The majority of his sea experience has been in US designed and built Guided Missile Frigates (FFG-7) and Destroyers (DDG-2). More interesting shore postings have included three years with NAVSEA in Washington DC working within the USN FFG-7 CDS Program and more recently, OIC of the RAN’s Test & Evaluation organisation in Sydney. Commander Curl has presided over the development of numerous Part Task Combat Systems Operations Training Simulators during his three years at WATSON and as a consequence, he is a strong advocate of the PC for use in this type of application.7.2 Mr Andrew Weisz, Serco Australia Pty LtdAndrew Weisz is the Contract Manager for WATSON’s on-site Systems Engineering Contractor, Serco Australia Pty Ltd. He has been employed in that capacity since 1991, prior to which he was the Technical Director with a small SCADA (Supervisory, Control and Data Acquisition) System manufacturer for 15 years. Since he has been at WATSON, he has applied the Man-Machine Interface design experience gained in his previous position to the development of numerous Part Task Trainers for the Royal Australian Navy. In 1995 he was the recipient of a Commanding Officer’s Commendation in recognition of his contribution to the development of training facilities at HMAS WATSON. Mr. Weisz has a Bachelor of Electrical Engineering degree from the University of New South Wales, Sydney Australia.REFERENCES[1] Curl, I., Weisz, A. Training Systems Development at HMAS WATSON. 1st International SimTecT Conference, March 25-26, 1996 - Melbourne Australia.[2] Sweet, P.E. Cost Effective Air Traffic Control Training Systems. 4th ITEC Conference, May 4-6, 1993 - London UK.[3] Moriarity, T.M. PC-Based Electronic Combat Simulators. 17th I/ITSEC Conference, November 13-16, 1995 - Albuquerque NM USA.[4] Sawler, R.J., Mielke, D.J. A Low-Cost Solution to Simulated Ground-Based Radar Systems. 17th I/ITSEC Conference, November 13-16, 1995 - Albuquerque NM USA.。