ITE_448906-42-1_DataSheet_MedChemExpress

合集下载

碧云天生物技术 Beyotime Biotechnology 生物素标记EMSA探针说明书

碧云天生物技术/Beyotime Biotechnology订货热线：400-168-3301或800-8283301订货e-mail：******************技术咨询：*****************网址：碧云天网站微信公众号生物素标记EMSA探针－β-Catenin/TCF (0.2μM)产品编号产品名称包装GS018B 生物素标记EMSA探针－β-Catenin/TCF (0.2µM) 200µl产品简介：生物素标记EMSA探针－β-Catenin/TCF是用于EMSA(也称gel shift)研究的生物素(Biotin)标记的β-Catenin/TCF consensus oligonucleotide。

这个生物素标记的双链寡核苷酸含有公认的β-Catenin/TCF结合位点，可以用作EMSA研究时的探针。

β-Catenin/TCF consensus oligo的序列如下：5'-CCC TTT GAT CTT ACC-3'3'-GGG AAA CTA GAA TGG-5'本生物素标记EMSA探针已经过纯化，可以直接用于EMSA结合反应。

本生物素标记EMSA探针可以和碧云天的化学发光法EMSA试剂盒(GS009)配套使用。

一个包装的生物素标记探针可以进行约200-400个样品的EMSA检测。

包装清单：产品编号产品名称包装GS018B 生物素标记EMSA探针－β-Catenin/TCF (0.2µM) 200µl—说明书1份保存条件：-20ºC保存，一年有效。

注意事项：避免加热到40ºC以上，温度过高会导致双链DNA探针解聚成单链。

而单链无法用于EMSA研究。

对于基于生物素标记的EMSA检测的详细操作可以参考碧云天的化学发光法EMSA试剂盒(GS009)的使用说明。

本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。

EL-102_SDS_MedChemExpress

Inhibitors, Agonists, Screening LibrariesSafety Data Sheet Revision Date:May-24-2017Print Date:May-24-20171. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifierProduct name :EL-102Catalog No. :HY-16187CAS No. :1233948-61-21.2 Relevant identified uses of the substance or mixture and uses advised againstIdentified uses :Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data sheetCompany:MedChemExpress USATel:609-228-6898Fax:609-228-5909E-mail:sales@1.4 Emergency telephone numberEmergency Phone #:609-228-68982. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureNot a hazardous substance or mixture.2.2 GHS Label elements, including precautionary statementsNot a hazardous substance or mixture.2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms:EL 102; EL102Formula:C19H16N2O3S2Molecular Weight:384.47CAS No. :1233948-61-24. FIRST AID MEASURES4.1 Description of first aid measuresEye contactRemove any contact lenses, locate eye-wash station, and flush eyes immediately with large amounts of water. Separate eyelids with fingers to ensure adequate flushing. Promptly call a physician.Skin contactRinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.InhalationImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.IngestionWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2).4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. FIRE FIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, dry chemical, foam, and carbon dioxide fire extinguisher.5.2 Special hazards arising from the substance or mixtureDuring combustion, may emit irritant fumes.5.3 Advice for firefightersWear self-contained breathing apparatus and protective clothing.6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresUse full personal protective equipment. Avoid breathing vapors, mist, dust or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.Refer to protective measures listed in sections 8.6.2 Environmental precautionsTry to prevent further leakage or spillage. Keep the product away from drains or water courses.6.3 Methods and materials for containment and cleaning upAbsorb solutions with finely-powdered liquid-binding material (diatomite, universal binders); Decontaminate surfaces and equipment by scrubbing with alcohol; Dispose of contaminated material according to Section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingAvoid inhalation, contact with eyes and skin. Avoid dust and aerosol formation. Use only in areas with appropriate exhaust ventilation.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly sealed in cool, well-ventilated area. Keep away from direct sunlight and sources of ignition.Recommended storage temperature:Powder-20°C 3 years4°C 2 yearsIn solvent-80°C 6 months-20°C 1 monthShipping at room temperature if less than 2 weeks.7.3 Specific end use(s)No data available.8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parametersComponents with workplace control parametersThis product contains no substances with occupational exposure limit values.8.2 Exposure controlsEngineering controlsEnsure adequate ventilation. Provide accessible safety shower and eye wash station.Personal protective equipmentEye protection Safety goggles with side-shields.Hand protection Protective gloves.Skin and body protection Impervious clothing.Respiratory protection Suitable respirator.Environmental exposure controls Keep the product away from drains, water courses or the soil. Cleanspillages in a safe way as soon as possible.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesAppearance Light yellow to yellow (Solid)Odor No data availableOdor threshold No data availablepH No data availableMelting/freezing point No data availableBoiling point/range No data availableFlash point No data availableEvaporation rate No data availableFlammability (solid, gas)No data availableUpper/lower flammability or explosive limits No data availableVapor pressure No data availableVapor density No data availableRelative density No data availableWater Solubility No data availablePartition coefficient No data availableAuto-ignition temperature No data availableDecomposition temperature No data availableViscosity No data availableExplosive properties No data availableOxidizing properties No data available9.2 Other safety informationNo data available.10. STABILITY AND REACTIVITY10.1 ReactivityNo data available.10.2 Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsNo data available.10.4 Conditions to avoidNo data available.10.5 Incompatible materialsStrong acids/alkalis, strong oxidising/reducing agents.10.6 Hazardous decomposition productsUnder fire conditions, may decompose and emit toxic fumes.Other decomposition products - no data available.11.TOXICOLOGICAL INFORMATION11.1 Information on toxicological effectsAcute toxicityClassified based on available data. For more details, see section 2Skin corrosion/irritationClassified based on available data. For more details, see section 2Serious eye damage/irritationClassified based on available data. For more details, see section 2Respiratory or skin sensitizationClassified based on available data. For more details, see section 2Germ cell mutagenicityClassified based on available data. For more details, see section 2CarcinogenicityIARC: No component of this product present at a level equal to or greater than 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.ACGIH: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by ACGIH.NTP: No component of this product present at a level equal to or greater than 0.1% is identified as a anticipated or confirmed carcinogen by NTP.OSHA: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by OSHA.Reproductive toxicityClassified based on available data. For more details, see section 2Specific target organ toxicity - single exposureClassified based on available data. For more details, see section 2Specific target organ toxicity - repeated exposureClassified based on available data. For more details, see section 2Aspiration hazardClassified based on available data. For more details, see section 212. ECOLOGICAL INFORMATION12.1 ToxicityNo data available.12.2 Persistence and degradabilityNo data available.12.3 Bioaccumlative potentialNo data available.12.4 Mobility in soilNo data available.12.5 Results of PBT and vPvB assessmentPBT/vPvB assessment unavailable as chemical safety assessment not required or not conducted.12.6 Other adverse effectsNo data available.13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsProductDispose substance in accordance with prevailing country, federal, state and local regulations.Contaminated packagingConduct recycling or disposal in accordance with prevailing country, federal, state and local regulations.14. TRANSPORT INFORMATIONDOT (US)This substance is considered to be non-hazardous for transport.IMDGThis substance is considered to be non-hazardous for transport.IATAThis substance is considered to be non-hazardous for transport.15. REGULATORY INFORMATIONSARA 302 Components:No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.SARA 313 Components:This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.SARA 311/312 Hazards:No SARA Hazards.Massachusetts Right To Know Components:No components are subject to the Massachusetts Right to Know Act.Pennsylvania Right To Know Components:No components are subject to the Pennsylvania Right to Know Act.New Jersey Right To Know Components:No components are subject to the New Jersey Right to Know Act.California Prop. 65 Components:This product does not contain any chemicals known to State of California to cause cancer, birth defects, or anyother reproductive harm.16. OTHER INFORMATIONCopyright 2017 MedChemExpress. The above information is correct to the best of our present knowledge but does not purport to be all inclusive and should be used only as a guide. The product is for research use only and for experienced personnel. It must only be handled by suitably qualified experienced scientists in appropriately equipped and authorized facilities. The burden of safe use of this material rests entirely with the user. MedChemExpress disclaims all liability for any damage resulting from handling or from contact with this product.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。

碧云天生物技术 Beyotime Biotechnology BeyoFusion

碧云天生物技术/Beyotime Biotechnology订货热线: 400-1683301或800-8283301订货e-mail:******************技术咨询: *****************网址: 碧云天网站微信公众号BeyoFusion™ DNA Polymerase产品编号产品名称包装D7220 BeyoFusion™ DNA Polymerase 200U产品简介：碧云天生产的BeyoFusion™ DNA Polymerase，是一种以嗜热古细菌DNA聚合酶(hyperthermophilic archaeon Pyrococcus-like DNA polymerase)为基础通过突变等改造而获得的超高性能DNA聚合酶，它具有扩增速度快、保真度极高、扩增片段可以轻松达到12kb等优点。

BeyoFusion™ DNA Polymerase扩增速度极快，扩增小于6kb的DNA片段时，延伸1kb只需要15秒(参考表1)。

普通的DNA聚合酶延伸1kb通常需要1-2分钟。

BeyoFusion™ DNA polymerase由于其超快的扩增速度，可以显著缩短PCR扩增所需的时间。

BeyoFusion™ DNA Polymerase是一种高保真DNA聚合酶。

BeyoFusion™ DNA Polymerase不仅可以非常高效地催化5'至3'方向的依赖于DNA模板的脱氧核苷酸的聚合反应，它同时还具有3'至5'的外切酶活性(proofreading activity)，它的错误发生概率比Taq酶要低52倍，比pfu酶要约低6倍(参考表1)。

表1. BeyoFusion™ DNA polymerase的主要性能与Taq酶及同类产品的比较。

Product Name Concentration Manufacture Velocity Target Size Fidelity Product end Taq 5U/μl Various 1min/kb <3kb 2.3×10-5/nt/cycle3'overhangs Pfu 5U/μl Various 2min/kb <5kb 2.6×10-6/nt/cycle Blunt Platinum Taq 5U/μl Thermo 30s/kb 15kb 3.8×10-6/nt/cycle 3'overhangs Phusion HF 2U/μl Thermo 15-30s/kb 20kb 4.4×10-7/nt/cycle BluntLongAmp Taq 2.5U/μl NEB 50s/kb 30kb 1.2×10-5/nt/cycle 3'overhangs Phusion HF 2U/μl NEB 15-30s/kb 20kb 4.6×10-7/nt/cycle BluntPfuUltra HF 2.5U/μl Agilent 1-2min/kb 17kb 1.3×10-6/nt/cycle BluntPfuUltra II FH - Agilent 15-30s/kb 19kb 1.2×10-6/nt/cycle BluntKOD Dash 2.5U/μl TOYOBO 30s/kb 18kb 5.8×10-6/nt/cycle 3'overhangsKOD FX 1U/μl TOYOBO 30s-1min/kb 40kb 2.1×10-6/nt/cycle BluntBeyoFusion™ 2.5U/μl Beyotime 15-60sec/kb >12kb 4.4×10-7/nt/cycle Blunt BeyoFusion™ Plus 2.5U/μl Beyotime 15-60sec/kb >12kb 2.2×10-6/nt/cycle 3' overhangs BeyoFusion™ DNA Polymerase的扩增长度长，可以达到12kb。

牛柠檬酸合成酶(CS)酶联免疫分析(ELISA)

牛柠檬酸合成酶(CS)酶联免疫分析（ELISA）试剂盒使用说明书本试剂仅供研究使用目的：本试剂盒用于测定牛血清，血浆及相关液体样本中肿瘤坏死因子相关凋亡诱导配体1(TRAIL-R1)的含量。

实验原理：本试剂盒应用双抗体夹心法测定标本中牛柠檬酸合成酶(CS)水平。

用纯化的牛柠檬酸合成酶(CS)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入肿瘤坏死因子相关凋亡诱导配体1(TRAIL-R1)，再与HRP标记的肿瘤坏死因子相关凋亡诱导配体1(TRAIL-R1)抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。

TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。

颜色的深浅和样品中的肿瘤坏死因子相关凋亡诱导配体1(TRAIL-R1)呈正相关。

用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中牛柠檬酸合成酶(CS)浓度。

试剂盒组成：试剂盒组成48孔配置96孔配置保存说明书1份1份封板膜2片（48）2片（96）密封袋1个1个酶标包被板1×48 1×96 2-8℃保存标准品：2700ng/L0.5ml×1瓶0.5ml×1瓶2-8℃保存标准品稀释液 1.5ml×1瓶 1.5ml×1瓶2-8℃保存酶标试剂 3 ml×1瓶 6 ml×1瓶2-8℃保存样品稀释液 3 ml×1瓶 6 ml×1瓶2-8℃保存显色剂A液 3 ml×1瓶 6 ml×1瓶2-8℃保存显色剂B液 3 ml×1瓶 6 ml×1瓶2-8℃保存终止液3ml×1瓶6ml×1瓶2-8℃保存浓缩洗涤液（20ml×20倍）×1瓶（20ml×30倍）×1瓶2-8℃保存样本处理及要求：1. 血清：室温血液自然凝固10-20分钟，离心20分钟左右（2000-3000转/分）。

迈勒泰尼生物技术说明书-MACS

.06Miltenyi Biotec B.V. & Co. KGpage 1/4Contents1. Description 1.1 Principle of the MACS® Separation 1.2 Background information 1.3 Applications1.4 Reagent and instrument requirements2. Protocol2.1 Sample preparation 2.2 Magnetic labeling 2.3 Magnetic separation2.4 C ell separation with the autoMACS® Pro Separator3. Example of a separation using CD45 (TIL) MicroBeadsWarningsReagents contain sodium azide. Under acidic conditions sodium azide yields hydrazoic acid, which is extremely toxic. Azide compounds should be diluted with running water before discarding. These precautions are recommended to avoid deposits in plumbing where explosive conditions may develop.1. DescriptionThis product is for research use ponents1 mL CD45 (TIL) MicroBeads, mouse:MicroBeads conjugated to monoclonal anti-mouse CD45 antibodies (isotype: rat IgG2b).CapacityFor 10⁹ total cells, up to 100 separations.Product format CD45 (TIL) MicroBeads are supplied in buffercontaining stabilizer and 0.05% sodium azide.StorageStore protected from light at 2−8 °C. Do not freeze. The expiration date is indicated on the vial label.1.1 Principle of the MACS® SeparationFirst, the CD45+cells are magnetically labeled with CD45 (TIL) MicroBeads. Then, the cell suspension is loaded onto a MACS® Column, which is placed in the magnetic field of a MACS Separator. The magnetically labeled CD45+ cells are retained within the column. The unlabeled cells run through; this cell fraction is thus depleted of CD45+ cells. After removing the column from the magnetic field, the magnetically retained CD45+ cells can be eluted as the positively selected cell fraction. To increase the purity, the positively selected cell fraction containing the CD45+ cells must be separated over a second column.1.2 Background informationCD45 (TIL) MicroBeads, mouse have been developed for theisolation of tumor-infiltrating leukocytes (TILs) from single-cell suspensions of solid mouse tumors. The CD45 antigen is expressed on all cells of hematopoietic origin except erythrocytes and platelets.1.3 Applications●Positive selection of CD45+ leukocytes from solid mouse tumors, e.g., B16F10, 4T1, or CT26.WT.1.4 Reagent and instrument requirements●Buffer: Prepare a solution containing phosphate-buffered saline (PBS), pH 7.2, 0.5% bovine serum albumin (BSA), and 2 mM EDTA by diluting MACS® BSA Stock Solution (# 130-091-376) 1:20 with autoMACS® Rinsing Solution (# 130-091-222). Keep buffer cold (2−8 °C). Degas buffer before use, as air bubbles could block the column. Always use freshly prepared buffer. ▲ Note: EDTA can be replaced by other supplements such as anticoagulant citrate dextrose formula-A (ACD-A) or citrate phosphate dextrose (CPD). BSA can be replaced by other proteins such as mouse serum albumin, mouse serum, or fetal bovine serum (FBS). Buffers or media containing Ca2+ or Mg2+ are not recommended for use.●MACS Columns and MACS Separators: CD45+ cells can be enriched by using MS or LS Columns. Positive selection can also be performed by using the autoMACS Pro or the MultiMACS™ Cell24 Separator.Positive selection MS 10⁷ 2 ×10⁷MiniMACS, OctoMACS, VarioMACS, SuperMACS II LS4 ×10⁷4 ×10⁷5 ×10⁷5 ×10⁷MidiMACS, QuadroMACS, VarioMACS, SuperMACS IIMultiMACS Cell24 Separator PlusautoMACS5 ×10⁷10⁸autoMACS ProMulti-24 Column Block (per column)2 ×10⁷2.5 ×10⁷MultiMACS Cell24 Separator Plus ▲Note: Column adapters are required to insert certain columns into the VarioMACS™ or SuperMACS™ II Separators. For details refer to the respective MACS Separator data sheet. ▲Note: If separating with LS Columns and the MultiMACS Cell24 Separator Plus use the Single-Column Adapter. Refer to the user manual for details.●Tumor Dissociation Kit, mouse (# 130-096-730) for the generation of single-cell suspension from tumor tissues.CD45 (TIL) MicroBeadsmouseOrder no. 130-110-618●gentleMACS™ Dissociator (# 130-093-235), gentleMACS OctoDissociator (# 130-095-937), or gentleMACS Octo Dissociatorwith Heaters (# 130-096-427)●gentleMACS C Tubes (# 130-093-237, # 130-096-334)●(Optional) Fluorochrome-conjugated REA (REAfinity™antibodies: recombinantly engineered, lacking Fcγ-bindingsite) CD45 antibodies for flow cytometric analysis, e.g., CD45-VioBlue®. For more information about antibodies refer to/antibodies.▲Note: Due to expression of Fcγ receptors on tumor-infiltrating leukocytesREA antibodies are recommended.●(Optional) Propidium Iodide Solution (# 130-093-233), DAPIStaining Solution (# 130-111-570), 7-AAD Staining Solution(# 130-111-568), or Viobility™ Fixable Dyes (# 130-109-812,# 130-109-814, # 130-109-816) for flow cytometric exclusion ofdead cells.●(Optional) Dead Cell Removal Kit (# 130-090-101) for thedepletion of dead cells.●(Optional) Pre-Separation Filters (30 µm) (# 130-041-407) toremove cell clumps.●(Optional) MACS SmartStrainers (30 µm) (# 130-098-458) toremove cell clumps.2. Protocol2.1 Sample preparationFor preparation of a single-cell suspension from solid mouse tumors use the Tumor Dissociation Kit, mouse (# 130-096-730) in combination with the gentleMACS™ Dissociators.For details refer to /protocols.▲ Dead cells may bind non-specifically to MACS® MicroBeads. To remove dead cells, we recommend using the Dead Cell Removal Kit (# 130-090-101).2.2 Magnetic labeling▲ Cells can be labeled with MACS MicroBeads using the autolabeling function of the autoMACS® Pro Separator. For more information refer to section 2.4.▲ Work fast, keep cells cold, and use pre-cooled solutions. This will prevent capping of antibodies on the cell surface and non-specific cell labeling.▲ Volumes for magnetic labeling given below are for up to 10⁷ total cells. When working with fewer than 10⁷ cells, use the same volumes as indicated. When working with higher cell numbers, scale up all reagent volumes and total volumes accordingly (e.g. for 2×10⁷ total cells, use twice the volume of all indicated reagent volumes and total volumes).▲ For optimal performance it is important to obtain a single-cell suspension before magnetic labeling. Pass cells through 30 µm nylon mesh (MACS SmartStrainers (30 µm), # 130-098-458). Moisten filter with buffer before use.▲ The recommended incubation temperature is 2–8 °C. Higher temperatures and/or longer incubation times may lead to non-specific cell labeling. Working on ice may require increased incubation times.1. Determine cell number.2. Centrifuge cell suspension at 300×g for 5 minutes. Aspiratesupernatant completely.3. Resuspend cell pellet in 90 µL of buffer per 10⁷ total cells.▲ Note: Always use freshly prepared buffer.4. Add 10 µL of CD45 (TIL) MicroBeads per 10⁷ total cells.5. Mix well and incubate for 15 minutes in the dark in therefrigerator (2−8 °C).6. (Optional) Add staining antibodies according tomanufacturer’s recommendations.7. Add buffer to a final volume of 500 μL for up to 5×10⁷ cells.▲Note: If more cells were used, split the sample onto multiple columns duringmagnetic separation.▲Note: For higher cell numbers, scale up buffer volume accordingly.8.Proceed to magnetic separation (2.3).2.3 Magnetic separation▲ Choose an appropriate MACS Column and MACS Separator according to the number of total cells and the number of CD45+ cells. For details refer to the table in section 1.4.▲ Note: MS Columns are recommended for highest purity of CD45+ cells. LSColumns are recommended for highest recovery of CD45+ cells.▲ For optimal performance it is important to obtain a single-cell suspension before magnetic separation. Pass cells through 30 µm nylon mesh (Pre-Separation Filters (30 µm), # 130-041-407) to remove cell clumps which may clog the column. Moisten filter with buffer before use.▲Always wait until the column reservoir is empty before proceeding to the next step.Magnetic separation with MS or LS Columns1. Place column in the magnetic field of a suitable MACSSeparator. For details refer to the respective MACS Columndata sheet.2. Prepare column by rinsing with the appropriate amount ofbuffer:MS: 500 µL LS: 3 mL3. Apply cell suspension onto the column. Collect flow-throughcontaining unlabeled cells.4. Wash column with the appropriate amount of buffer. Collectunlabeled cells that pass through and combine with theflow-through from step 3.MS: 3×500 µL LS: 2×1 mL▲ Note:Perform washing steps by adding buffer aliquots as soon as the columnreservoir is empty.5. Remove column from the separator and place it on a suitablecollection tube.6. Pipette the appropriate amount of buffer onto the column.Immediately flush out the magnetically labeled cells by firmly pushing the plunger into the column.MS: 1 mL LS: 3 mL7. (Optional) To increase the purity of CD45+cells, the elutedfraction can be enriched over a second MS or LS Column.Repeat the magnetic separation procedure as described in steps 1 to 6 by using a new column.Magnetic separation with the MultiMACS™ Cell24 Separator Refer to the the MultiMACS™ Cell Separator user manual for instructions on how to use the MultiMACS Cell24 Separator.2.4 Cell separation with the autoMACS® Pro Separator▲Refer to the user manual for instructions on how to use the autoMACS® Pro Separator.▲ All buffer temperatures should be ≥10 °C.▲ For appropriate resuspension volumes and cell concentrations, please visit /autolabeling.▲ Place tubes in the following Chill Rack positions:position A = sample, position B = negative fraction,position C = positive fraction.2.4.1 F ully automated cell labeling and separation1. Switch on the instrument for automatic initialization.2. Go to the Reagent menu and select Read Reagent. Scan the2D barcode of each reagent vial with the barcode scanner on the autoMACS® Pro Separator. Place the reagent into the appropriate position on the reagent rack.3. Place sample and collection tubes into the Chill Rack.4. G o to the Separation menu and select the reagent name foreach sample from the Labeling submenu (the correct labeling, separation, and wash protocols will be selected automatically).5. Enter sample volume into the Volume submenu. Press Enter.6. Select Run.2.4.2 M agnetic separation using manual labeling1. Label the sample as described in section2.2 Magnetic labeling.2. Prepare and prime the instrument.3. Apply tube containing the sample and provide tubes forcollecting the labeled and unlabeled cell fractions. Place sample and collection tubes into the Chill Rack.4. For a standard separation choose one the following programs:Positive selection:Posseld2for highest purityorPossels for highest recoveryCollect positive fraction in row C of the tube rack.3. Example of a separation usingCD45 (TIL) MicroBeadsA tumor induced by the B16F10 cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD45+ TILs were isolated from the single-cell suspension using CD45 (TIL) MicroBeads, an MS Column, and a MiniMACS™ Separator.Cells were fluorescently stained with CD45-PE and Labeling-Check-Reagent-VioBlue® and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.Before separation10³-1110¹10²10³10²10¹CD45-PELabelingCheckReagent-VioBlue-11CD45+ cells10³-1110¹10²10³10²10¹CD45-PELabelingCheckReagent-VioBlue-11Refer to for all data sheets and protocols. Miltenyi Biotec provides technical support worldwide. Visit /local to find your nearest Miltenyi Biotec contact.Legal noticesLimited product warrantyMiltenyi Biotec B.V. & Co. KG and/or its affiliate(s) warrant this product to be free from material defects in workmanship and materials and to conform substantially with Miltenyi Biotec’s published specifications for the product at the time of order, under normal use and conditions in accordance with its applicable documentation, for a period beginning on the date of delivery of the product by Miltenyi Biotec or its authorized distributor and ending on the expiration date of the product’s applicable shelf life stated on the product label, packaging or documentation (as applicable) or, in the absence thereof, ONE (1) YEAR from date of delivery (“Product Warranty”). Miltenyi Biotec’s Product Warranty is provided subject to the warranty terms as set forth in Miltenyi Biotec’s G eneral Terms and Conditions for the Sale of Products and Services available on Miltenyi Biotec’s website at , as in effect at the time of order (“Product Warranty”). Additional terms may apply. BY USE OF THIS PRODUCT, THE CUSTOMER AGREES TO BE BOUND BY THESE TERMS.THE CUSTOMER IS SOLELY RESPONSIBLE FOR DETERMINING IF A PRODUCT IS SUITABLE FOR CUSTOMER’S PARTICULAR PURPOSE AND APPLICATION METHODS.Technical informationThe technical information, data, protocols, and other statements provided by Miltenyi Biotec in this document are based on information, tests, or experience which Miltenyi Biotec believes to be reliable, but the accuracy or completeness of such information is not guaranteed. Such technical information and data are intended for persons with knowledge and technical skills sufficient to assess and apply their own informed judgment to the information. Miltenyi Biotec shall not be liable for any technical or editorial errors or omissions contained herein.All information and specifications are subject to change without prior notice. Please contact Miltenyi Biotec Technical Support or visit for the most up-to-date information on Miltenyi Biotec products.LicensesThis product and/or its use may be covered by one or more pending or issued patents and/or may have certain limitations. Certain uses may be excluded by separate terms and conditions. Please contact your local Miltenyi Biotec representative or visit Miltenyi Biotec’s website at for more information.The purchase of this product conveys to the customer the non-transferable right to use the purchased amount of the product in research conducted by the customer (whether the customer is an academic or for-profit entity). This product may not be further sold. Additional terms and conditions (including the terms of a Limited Use Label License) may apply.CUSTOMER’S USE OF THIS PRODUCT MAY REQUIRE ADDITIONAL LICENSES DEPENDING ON THE SPECIFIC APPLICATION. THE CUSTOMER IS SOLELY RESPONSIBLE FOR DETERMINING FOR ITSELF WHETHER IT HAS ALL APPROPRIATE LICENSES IN PLACE. Miltenyi Biotec provides no warranty that customer’s use of this product does not and will not infringe intellectual property rights owned by a third party. BY USE OF THIS PRODUCT, THE CUSTOMER AGREES TO BE BOUND BY THESE TERMS.TrademarksautoMACS, gentleMACS, MACS, MACSQuant, MidiMACS, the Miltenyi Biotec logo, MiniMACS, MultiMACS, OctoMACS, QuadroMACS, REAfinity, SuperMACS, VarioMACS, Viobility, and VioBlue are registered trademarks or trademarks of Miltenyi Biotec and/or its affiliates in various countries worldwide.Copyright © 2020 Miltenyi Biotec and/or its affiliates. All rights reserved.。

安捷伦产品目录

15
Real-Time PCR
16
Mx3000P QPCR System
17
Brilliant III Ultra-Fast SYBR Green QPCR and QRT-PCR Reagents
18
Brilliant III Ultra-Fast QPCR and QRT-PCR Reagents
Agilent / STRATAGENE
Agilent website: /genomics
Welgene | Agilent Stratagene
威健股份有限公司 | Stratagene 總代理
Table of Content
Table of Contents
/ XL1-Red Competent Cells SoloPack Gold Supercompetent Cells
/ TK Competent Cells Specialty Cells
/ Classic Cells / Fine Chemicals For Competent Cells
適用於 UNG 去汙染或 bisulphite
sequencing
適用於 TA Cloning
最高敏感性
取代傳統 Taq 的好選擇
-
2
威健股份有限公司 | Stratagene 總代理
PCR Enzyme & Instrument
Agilent SureCycler 8800
市場上領先的 cycling 速度和 sample 體積 10 ～ 100 μL 簡易快速可以選擇 96 well 和 384 well 操作盤優秀的溫控設備讓各個 well 都能保持溫度的穩定七吋的高解析度觸控螢幕讓操作上更為簡便可以透過網路遠端操控儀器及監控儀器 Agilent 專業的技術支援可以幫助您應對各種 PCR 的問題

超高效液相色谱-串联质谱法测定化妆品中15种N-亚硝胺化合物

第42 卷第 11 期2023 年11 月Vol.42 No.111469~1478分析测试学报FENXI CESHI XUEBAO（Journal of Instrumental Analysis）超高效液相色谱-串联质谱法测定化妆品中15种N-亚硝胺化合物汪毅1，梁文耀1，何国山1，陈张好2，周智明2，吴谦1，席绍峰1，谭建华1*（1．广州质量监督检测研究院，国家化妆品质量检验检测中心（广州），广东广州511447；2．广东省药品检验所，广东广州510663）摘要：采用超高效液相色谱-串联质谱（UPLC-MS/MS）建立了化妆品中15种痕量N-亚硝胺化合物的分析方法。

水剂样品以水或乙腈分组超声提取，膏霜乳液样品采用亚铁氰化钾-乙酸锌溶液沉淀大分子或者饱和氯化钠-乙腈盐析分组处理后，以Agilent Poroshell 120 SB-Aq（100 mm×3.0 mm，2.7 μm）色谱柱分离，经大气压化学电离源（APCI）电离，多反应监测模式检测，以同位素内标法定量。

结果表明，15种N-亚硝胺化合物在相应质量浓度范围内线性关系良好（r2＞0.995），检出限和定量下限分别为5~15 ng/g和15~45 ng/g。

水、乳、膏霜3种化妆品基质在25、50、100 ng/g加标水平下的平均回收率为88.0%~111%，相对标准偏差（RSD，n=6）为1.4%~9.8%。

该方法用于市售化妆品检测，发现13批次样品检出N-亚硝基二乙醇胺（NDELA），其中1批次超限量值。

方法的专属性强，灵敏度高，精密度好，解决了N-亚硝胺化合物稳定性差、易被干扰等问题，适用于化妆品中15种N-亚硝胺化合物的痕量测定。

关键词：N-亚硝胺化合物；化妆品；超高效液相色谱-串联质谱法（UPLC-MS/MS）；大气压化学电离源中图分类号：O657.63；O623.732文献标识码：A 文章编号：1004-4957（2023）11-1469-10 Determination of Fifteen N-nitrosamine Compounds in Cosmetics by Ultra Performance Liquid Chromatography-TandemMass SpectrometryWANG Yi1，LIANG Wen-yao1，HE Guo-shan1，CHEN Zhang-hao2，ZHOU Zhi-ming2，WU Qian1，XI Shao-feng1，TAN Jian-hua1*（1．Guangzhou Quality Supervision and Testing Institute，National Quality Supervision and Testing Center for Cosmetics（Guangzhou），Guangzhou　511447，China；2．Guangdong Institute for Drug Control，Guangzhou　510663）Abstract：An ultra performance liquid chromatography-tandem mass spectrometric（UPLC-MS/MS）method was established for detecting 15 trace N-nitrosamine compounds in cosmetics. The final estab⁃lished method involved ultrasonic extraction of cosmetics using water or acetonitrile for different com⁃pounds. The samples were treated with potassium ferrocyanide-zinc acetate solution for precipitating macromolecules or saturated sodium chloride-acetonitrile for salting out.An Agilent Poroshell 120 SB-Aq（100 mm × 3.0 mm，2.7 μm） chromatography column was used for separation，followed by atmospheric pressure chemical ionization（APCI） source and multiple reaction monitoring mode detec⁃tion in the isotope internal standard method for quantification. The result showed good linearity（r2> 0.995） for the 15 N-nitrosamine compounds in their respective concentration ranges，with detection and quantitation limits of 5-15 ng/g and 15-45 ng/g，respectively.The average recoveries for the three cosmetic matrices（aqueous，emulsion，cream） at spiked levels of 25，50，100 ng/g were be⁃tween 88.0% and 111%，with relative standard deviations（RSD，n=6） of 1.4%-9.8%. The method was applied to the detection of commercial cosmetics and N-nitrosodiethanolamine（NDELA） was de⁃tected in 13 batches，with one batch exceeding the limit. The strong specificity，high sensitivity，and good precision made the method could solve the problems of poor stability and easy interference ofdoi：10.19969/j.fxcsxb.23051602收稿日期：2023－05－16；修回日期：2023－06－10基金项目：广东省药品监督管理局化妆品风险评估重点实验室专项（2021ZDZ03）；广东省市场监督管理局科技项目（2022CZ06）∗通讯作者：谭建华，博士，正高级工程师，研究方向：色谱－质谱检测技术研究，E-mail：tanjianhua0734@第 42 卷分析测试学报N-nitrosamine compounds，and was suitable for the trace determination of 15 N-nitrosamine com⁃pounds in cosmetics.Key words：N-nitrosamine compounds；cosmetics；ultra performance liquid chromatography-tan⁃dem mass spectrometry（UPLC-MS/MS）；atmospheric pressure chemical ionization（APCI） sourceN-亚硝胺化合物是一类具有N-亚硝基结构的化合物，因取代基的不同，形成了种类繁多的同系物，目前已发现超过300种［1］。

加速溶剂萃取-超高效液相色谱-串联质谱法测定大枣中3种五环三萜酸

食品与药品Food and Drug2021年第23卷第1期17加速溶剂萃取-超高效液相色谱-串联质谱法测定大枣中3种五环三祜酸张萍，何婷，王颖，胡克特，顾丁，陈荣祥**(遵义医科大学基础医学院，贵州遵义563000)摘要：目的建立加速溶剂萃取-超高效液相色谱-串联质谱法(ASE-UPLC-MS/MS)同时测定大枣中桦木酸、齐墩果酸和熊果酸的方法。

方法样品釆用ASE提取，优化提取条件，并与超声辅助提取法进行比较。

优化后的提取条件为：以80%甲醇为提取溶剂，提取温度100-C,静态萃取时间15min,萃取1次。

提取液釆用Waters ACQUITY BEH C18色谱柱分离，以乙ffi-15mmol/L乙酸钱(pH9.3)为流动相，梯度洗脱，经UPLC-MSZMS仪，釆用电喷雾电离源，负离子模式下多反应监测模式检测。

结果桦木酸、齐墩果酸、熊果酸在0.5~10 mg/L范围内，浓度与峰面积线性关系良好，相关系数大于0.9900。

不同浓度3种化合物的加标回收率93.6%〜101.7%,相对标准偏差在1.18%~6.83%之间。

结论此法简单快速、准确稳定、重复性好，可用于大枣中桦木酸、齐墩果酸、熊果酸的含量测定。

关键词：加速溶剂萃取；超高效液相色谱；串联质谱法；大枣中图分类号：R284.1文献标识码：A文章编号：1672-979X(2021)01-0017-06DOI：10.3969/j.issn.l672-979X.2021.01.004Simultaneous Determination of Three Pentacyclic Triterpenic Acids in Jujubae Fructus byAccelerated Solvent Extraction-UPLC-MS/MSZHANG Ping,HE Ting,WANG Ying,HU Ke-te,GU Ding,CHEN Rong-xiang(School of B asic Medical Sciences,Zunyi Medical University,Zu^yi563000,China)Abstract:Objective To establish a method for the simultaneous determination of betulinic acid,oleanolic acid and ursolic acid in Jujubae fructus by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)with accelerated solvent extraction(ASE).Methods The extract parameters of A SE were optimized and the efficiency was compared with the ultrasound-assisted extraction method.The optimum extraction conditions were as follows:80%methanol was selected as extraction solvent,oven temperature was100°C,the static extraction time was 15min and one extraction cycle was adopted.A waters ACQUITY BEH C18(2.1mmx]00mn,1.7“m)column was used as the stationary phase,acetonitrile and ammonium acetate solution(15mmol/L,pH9.3)was used as the mobile phase.Mass detection was conducted by electrospray ionization in negative ion multiple reaction monitoring mode. Results The calibration curves were linear over a concentration range of0.5-10mg/L for betulinic acid,oleanolic acid and ursolic acid.The correlation coefficients were greater than0.9900.The recoveries of different spiked levels were between93.6%and101.7%,with RSDs between1.18%and6.83%.Conclusion The method is simple,rapid,收稿日期：2020-09-04基金项目：国家自然科学基金(31660131,81760652)；贵州省联合基金(黔科合J字LKZ[2013]17号)；遵义医学院博士启动基金(F-568)作者简介：张萍，硕士研究生，研究方向：药用植物开发与利用E-mail:******************通讯作者：陈荣祥，教授，博士，研究方向：药用植物开发与利用E-mail:*************************18食品与药品Food and Drug2021年第23卷第1期accurate,stable and reproducible.It can be used for the determination of betulinic acid,oleanolic acid and ursolic acid in Jujubae f ructus.Key Words:accelerated solvent extraction;ultra-high performance liquid chromatography;tandem mass spectrometry; Jujubae f ructus.大枣为鼠李科植物枣树（Ziziphus jujuba Mill.）的干燥成熟果实，不仅作为食物，还是传统中医药中的常用药材。

IFCC Aspartate Aminotransferase 检测手册说明书

ASTAspartate Aminotransferase IFCCMANUAL RX MONZAINTENDED USEFor the quantitative in vitro determination of AspartateAminotransferase (AST) in serum and plasma. This product is suitable for manual use and on the Rx Monza analyser.Cat. No. AS 1202 R1a. Buffer/Substrate 1 x 70 ml 20 x 2 ml R1b. Enzyme/Coenzyme/ 20 x 2 ml α-oxoglutarate GTIN: 05055273200416AS 1204 R1a. Buffer/Substrate 1 x 105 ml 10 x 10 ml R1b. Enzyme/Coenzyme/ 10 x 10 ml α-oxoglutarate GTIN: 05055273200423AS 1267 R1a. Buffer/Substrate 1 x 105 ml 5 x 20 ml R1b. Enzyme/Coenzyme/ 5 x 20 ml α-oxoglutarate GTIN: 05055273200430AS 2359 R1a. Buffer/Substrate 5 x 100 ml 5 x 100 ml R1b. Enzyme/Coenzyme/ 5 x 100 ml α-oxoglutarate GTIN: 05055273200454UV METHODThis is an optimised standard method according to the concentrations recommended by the IFCC.CLINICAL SIGNIFICANCE (1,2,3,4)The aminotransferases are a group of enzymes that catalyse the inter conversions of amino acids and α-oxoacids by transfer of amino groups. AST (aspartate aminotransferase or glutamate oxaloacetatetransaminase) has been found in the cytoplasm and the mitochondria of cells that have been studied. In cases of mild tissue damage, e.g. liver, the predominant form of serum AST is that from the cytoplasm, with a smaller amount coming from the mitochondria. Severe tissue damage will result in more mitochondrial enzyme being released. Elevated levels of AST can signal myocardial infarction, hepatic disease, muscular dystrophy and organ damage.Although heart muscle is found to have the most activity of the enzyme, significant activity has also been seen in the brain, liver, gastric mucosa, adipose tissue and kidneys of humans.The IFCC has now recommended (1980) standardised procedures for AST determinations including:-1. optimization of substrate concentrations.2. Employment of Tris buffers (instead of phosphate, which has beenshown to inhibit recombination of the apoenzyme with pyridoxal phosphate).3. Pre-incubation of combined buffer and serum to allow sidereactions with NADH to occur. 4. Substrate start (α-oxoglutarate)5. Optional pyridoxal phosphate activation.This is an optimised standard method according to the recommendations of the IFCC.PRINCIPLEα-oxoglutarate reacts with L-aspartate in the presence of AST to form L-glutamate plus oxaloacetate. The indicator reaction utilises the oxaloacetate for a kinetic determination of NADH consumption. AST -oxoglutarate + L-aspartate L-glutamate + oxaloacetate MDH oxaloacetate + NADH + H + L-malate + NAD +SPECIMEN COLLECTION AND PREPARATION (5) Serum:- Use serum free from haemolysis.Plasma:- EDTA or heparin can be used as the anticoagulant.Plasma should be separated from cells within one hour after collection.Specimens should be refrigerated if not used immediately:-Specimens stored longer than 3 days should be frozen at -20︒C.REAGENT COMPOSITIONContents Concentrations in the TestR1a. Buffer/Substrate Tris buffer 80 mmol/l, pH 7.5 L-aspartate 240 mmol/l R1b. Enzyme/Coenzyme/α-oxoglutarate α-oxoglutarate 12 mmol/l MDH ≥420 U/l LD ≥600 U/l NADH 0.18 mmol/lSAFETY PRECAUTIONS AND WARNINGS For in vitro diagnostic use only. Do not pipette by mouth.Exercise the normal precautions required for handling laboratory reagents.Solution R1a contains Sodium Azide. Avoid ingestion or contact with skin or mucous membranes. In case of skin contact, flush affected area with copious amounts of water. In case of contact with eyes or if ingested, seek immediate medical attention.Sodium Azide reacts with lead and copper plumbing, to form potentially explosive azides. When disposing of such reagents flush with large volumes of water to prevent azide build up. Exposed metal surfaces should be cleaned with 10% sodium hydroxide.Health and Safety data sheets available on request.The reagents must be used only for the purpose intended by suitably qualified laboratory personnel, under appropriate laboratory conditions.STABILITY AND PREPARATION OF REAGENTS R1a. Buffer/SubstrateContents ready for use. Stable up to the expiry date when stored at +2 to +8︒C.R1b. Enzyme/Coenzyme/α-oxoglutarate Reconstitute one vial of Enzyme/Coenzyme/α-oxoglutarate R1b with the appropriate volume of Buffer/Substrate R1a: 2 ml for the 20 x 2 ml kit (AS 1202) 10 ml for the 10 x 10 ml kit (AS 1204) 20 ml for the 5 x 20 ml kit (AS 1267) Stable for 14 days at +2 to +8︒C or 24 hours at +15 to +25︒C. Cat. AS 2359 5 x 100 mlReconstitute one vial of Enzyme/Coenzyme/α-oxoglutarate R1b with a portion of Buffer/Substrate R1a and then transfer the entire contents to bottle R1a rinsing bottle R1b several times. Stable for 14 days at +2 to +8︒C or 24 hours at +15 to +25︒C.MATERIALS PROVIDED Buffer/SubstrateEnzyme/Coenzyme/ -oxoglutarateMATERIALS REQUIRED BUT NOT PROVIDEDRandox Assayed Multisera Level 2 (Cat. No. HN 1530) and Level 3 (Cat. No. HE 1532)Randox Calibration Serum Level 3 (Cat. No. CAL 2351) RX series Saline (Cat. No. SA 3854)PROCEDUREAspirate fresh ddH 2O and perform a new Gain Calibration in flow cell mode. Select AST in the Run Test screen and carry out a water blank as instructed.Pipette into a test tube:Sample 0.05 ml Reagent 0.5 mlMix and aspirate into the Rx Monza.CALIBRATION FOR RX MONZAThe use of Saline and Randox Calibration Serum Level 3 isrecommended for calibration. Calibration is recommended with change of reagent lot or as indicated by quality control procedures.FOR MANUAL USEWavelength: 340 nm (Hg 334 nm or Hg 365 nm) Cuvette: 1 cm light path Temperature: 25/30/37︒C Measurement: against airPipette into cuvette: Macro MicroSample 0.2 ml 0.1 ml Enzyme/Coenzyme/ α-oxoglutarate R1 2.0 ml 1.0 mlMix, read initial absorbance after 1 minute. Read again after 1, 2 and 3 minutes. Note: If the absorbance change per minute is between 0.11 and 0.16 at 340/Hg 334 nm 0.06 and 0.08 at Hg 365 nmuse only the values for the first 2 minutes for the calculation.MANUAL CALCULATIONTo calculate the AST activity, use the following formulae:U/l = 1746 x A 340 nm/min U/l = 1780 x A Hg 334 nm/min U/l = 3235 x A Hg 365 nm/minSTANDARDISATIONRandox Calibration Serum Level 3 is traceable to AST reference material JSCC TS01.QUALITY CONTROLRandox Assayed Multisera, Level 2 and Level 3 are recommended for daily quality control. Two levels of controls should be assayed at least once a day. Values obtained should fall within a specified range. If these values fall outside the range and repetition excludes error the following steps should be taken:1. Check instrument settings and light source.2. Check cleanliness of all equipment in use.3. Check water. Contaminants, i.e. bacterial growth, maycontribute to inaccurate results. 4. Check reaction temperature.5. Check expiry date of kit and contents.6. Contact Randox Laboratories Customer Technical Services, Northern Ireland +44 (0) 28 9445 1070.SPECIFICITY/INTERFERENCE (6,7)Gross haemolysis will produce falsely elevated test results. The effects of various drugs on AST activity should be taken intoconsideration in the case of patients receiving large doses of drugs.The analytes below were tested up to the following levels and were found not to interfere: Haemoglobin 250 mg/dl Free Bilirubin 25 mg/dl Conjugate Bilirubin 25 mg/dl Triglycerides 1000 mg/dlIntralipid ® 200 mg/dlA list of substances and conditions known to effect AST activity in vivo is given by both Young et al and Friedman et al. Norepresentation is made by Randox Laboratories Ltd regarding the completeness of these lists and the accuracy of the information contained therein.NORMAL VALUES IN SERUM (8,9) +25︒C +30︒C +37︒C Men up to 18 U/l up to 25 U/l up to 37 U/l Women up to 15 U/l up to 21 U/l up to 31 U/lIt is recommended that each laboratory establish its own reference range to reflect the age, sex, diet and geographical location of the population.SPECIFIC PERFORMANCE CHARACTERISTICS The following performance data were obtained using an Rx Monza analyser running at +37o C.LINEARITYThis method is linear up to 562 U/l. If the sample concentration exceeds this value, dilute the sample 1+9 with 0.9% NaCl solution and re-assay. Multiply the result by 10.SENSITIVITYThe minimum detectable concentration of AST with an acceptable level of precision was determined as 9.3 U/l.PRECISIONIntra AssayLevel 2 Level 3Mean (U/l) 35.6 153SD 1.66 1.47CV(%) 4.65 0.96n 20 20Inter AssayLevel 2 Level 3Mean (U/l) 35.6 153SD 1.77 7.10CV(%) 4.96 4.63n 20 20CORRELATIONThis method (Y) was compared with another commerciallyavailable method (X) and the following linear regression equationobtained:Y = 1.07X + 4.9and a correlation coefficient of r = 0.997543 patient samples were analysed spanning the range 28 to 559U/l.REFERENCES1. Wroblewski F, La Due J.S: Ann Intern Med. 1956; 45: 801.2. Wroblewski F, La Due J.S: Proc Soc Exp Biol Med 1956;91: 569.3. Bergmeyer HU, Bowers GN Jr, et al: Clin Chem 1977; 23:887.4. Bergmeyer HU, Bowers GN Jr, et al: J.Clin Chem ClinBiochem 1980; 18: 521-534.5. Tietz N W: Fundamentals of Clinical Chemistry ed 3.Philadelphia, WB Saunders Co. 1987, pg 372.6. Young D S, et al: Clin Chem 1975, 21; No5.7. Friedman RB, et al: Clin Chem 1980, 26; No4.8. Wallnofer H, Schmidt.E, Schmidt FW, eds: Synopsis derLeberkrankheiten Stuttgart, Georg Thieme Verlag, 1974.9. Thefeld W, et al: Dtsch Med Wschr 1974; 99: 343.Revised 26 Apr 16 biRev. 003THIS PAGE IS INTENTIONALLY BLANK。

细胞内钙离子的检测方法

产品说明书
细胞内钙离子检测试剂盒（BBcellProbeTM F3 法）
产品组成：
产品编号规格
钙离子染色液
BB-48112-1 50 -500T 25ul
BB-48112-2 100 -1000T
50ul
储存条件： -学研究使用，不可用于诊断或治疗。 ● 螺旋盖微量试剂管装的试剂在开盖前请短暂离心，将盖和管内壁上的液体离心至管底，避免开盖时试剂损失。 ● 样品或试剂被细菌或真菌污染或试剂交叉污染可能会导致错误的结果。 ● 最好使用一次性吸头、管、瓶或玻璃器皿，可重复使用的玻璃器皿必须在使用前清洗并彻底清除残留清洁剂。 ● 避免皮肤或粘膜与试剂接触。 ● 钙离子染色液为 DMSO 溶液，冬季气温较低时在室温时为凝固状态，极易粘附在管壁、吸头壁。注意需要加热溶解，吸头也需要放在培养箱预热，否者容易再次凝固在吸头内壁产生损耗。 ● 荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。
波长 526nm。
相关产品：
产品 Annexin V-FITC/PI 凋亡试剂盒 Annexin V-EGFP/PI 凋亡试剂盒 MTT 细胞增殖及毒性检测试剂盒 CCK-8 细胞增殖毒性检测试剂盒 WST-1 细胞增殖毒性检测试剂盒 MTS 细胞增殖与毒性检测试剂盒 Hoechst33342/PI 双染试剂盒 DAPI 染色试剂盒细胞存活率检测试剂盒
定最佳条件。以下方法仅供参考。 ● 可以在染色溶液中加入等体积的 20% Pluronic F127 溶液，Pluronic F127 可以帮助 BBcellProbeTM
F3 更快进入细胞，不建议在 Pluronic F127 中长期保存 BBcellProbeTM F3 溶液。
1、染色工作液的配制：用 HBSS 稀释 BBcellProbeTM F3 溶液 400 倍-4000 倍，配制成 BBcellProbeTM F3 染色工作液。【注】：推荐该探针加载浓度在 400-4000 倍稀释，具体的使用浓度需根据实验要求进行优化。为了避免过度加载造成细胞毒性，建议在取得有效结果的基础上尽量使用最低探针浓度。

PfuTurbo Cx Hotstart DNA Polymerase 说明书

PfuTurbo Cx Hotstart DNA Polymerase, Part Number 600410*************(24小时)化学品安全技术说明书GHS product identifier 应急咨询电话（带值班时间）::供应商/ 制造商:安捷伦科技贸易（上海）有限公司中国（上海）外高桥自由贸易试验区英伦路412号（邮编:200131)电话号码： 800-820-3278传真号码: 0086 (21) 5048 2818PfuTurbo Cx Hotstart DNA Polymerase, Part Number 600410化学品的推荐用途和限制用途DMSO 600260-53PfuTurbo Cx Hotstart DNA Polymerase 600410-5110X PfuTurbo Cx Reaction Buffer 600410-52部件号:部件号（化学品试剂盒）:600410安全技术说明书根据 GB/ T 16483-2008 和 GB/ T 17519-2013GHS化学品标识:PfuTurbo Cx 热启动 DNA 聚合酶，货号 600410推荐用途DMSO 1 mlPfuTurbo Cx Hotstart DNA Polymerase40 μl (100 U 2.5 U/µl)600410-5210X PfuTurbo Cx Reaction Buffer4 x 1 ml:紧急情况概述DMSO 液体。

[透明。

]PfuTurbo Cx Hotstart DNA Polymerase液体。

10X PfuTurbo Cx Reaction Buffer 液体。

DMSO 无色。

PfuTurbo Cx Hotstart DNA Polymerase无资料。

10X PfuTurbo Cx Reaction Buffer 无资料。

DMSO 无气味的。

[轻微]PfuTurbo Cx Hotstart DNA Polymerase无资料。

PH值测试预装试剂Method photometric pH 6.4 - 8.8 Spectroq

Millipore- 1.01744页码 1 的 6The life science business of Merck operates as MilliporeSigma in the US化学品安全技术说明书按照GB/T 16483、GB/T 17519编制版本8.2修订日期12.10.2022打印日期21.10.2022最初编制日期23.03.2022SDS 编号Millipore - 1.01744产品编号Millipore - 1.01744PH值测试预装试剂 Method: photometric pH 6.4 - 8.8 Spectroquant®第1部分：化学品及企业标识1.1 产品标识产品名称: PH值测试预装试剂 Method: photometric pH 6.4 -8.8 Spectroquant®pH Cell Test Method: photometric pH 6.4 -8.8 Spectroquant®产品编号: 1.01744产品编号: 101744品牌: Millipore1.2 安全技术说明书提供者的详情制造商或供应商名称: Sigma-Aldrich (Shanghai) Trading Co.Ltd.509 Renqing RoadZhangjiang High Tech East Park, PudongSHANGHAI201201 SHANGHAICHINA西格玛奥德里奇（上海）贸易有限公司上海市浦东新区仁庆路509号10幢邮政编码：201201默克股份两合公司64271 达姆施塔特德国Millipore- 1.01744页码 2 的 6The life science business of Merck operates as MilliporeSigma in the USPhone:+49(0)6151 72-2440电话号码: +86 21 6141-5566传真: +86 21 6141-55671.3 应急咨询电话紧急联系电话: +86 532 838890901.4 物质或混合物的推荐用途和限制用途已确认的各用途: 研发用试剂这是此产品概括的物质安全资料表(SDS),如需第16项中所列的每一成分完整的物质安全资料表(SDS),请浏览我们的网站.第2部分：危险性概述2.1 GHS危险性类别非危险物质或混合物。

练习使用SciFinder Schlor 检索

练习使用SciFinder Schlor 检索Chemical Abstract数据库一、练习Explore、Locate、和Browse检索。

检索物质：富马酸二甲酯（Dimethyl Fumarate）A .Explore<略>B .Locatelocate liturature<ii>locate substances检索dimethyl fumarate，得到结构式如右C .Browse在“Journals 505 -513 of 2128”中查看Crystal期刊 volume 2 2012二、自选题目进行Explore下的各项检索，并进行Analyze or Refine，提交检索报告（检索内容，和结论）。

检索物质：富马酸二甲酯（Dimethyl Fumarate）A .Explore LiteratureResearch Topic1924 references were found containing "dimethyl fumarate" as entered.3297 references were found containing the concept "dimethyl fumarate".共有4221条检索结果摘录第一篇Zou, Jie; Zhang, Zheng; Yan, Chunrong; Lu, Jian. Determination ofdimethyl fumarate in pastry and spicy snacks by GPC-GC/MS.Shipin Kexue(Beijing, China) (2011), 32(24), 267-269. CODEN: SPKHD5 ISSN:1002-6630. AN2012:400892 CAPLUS [1]<ii>Author Name检索Zou, Jie; 共有41条检索结果，得到进入选中作者的条目中，reference中第一篇即同中摘录文章相同<iii>Company Name/Organizaton[1]文章摘录和原文网址附在附录1检索Shipin Kexue，共有一条结果：Xu, Honghua. The relationship between diet andnutrition and cancer.Shipin Kexue (Beijing) (1996), 17(6), 57-59. CODEN: SPKHD5ISSN:1002-6630. CAN 126:329885 AN 1997:316317 CAPLUS检索结果与中发刊机构相同B .Explore SubsatncesChemical StructureExact search检出 680个物质Substructure search检出 45739 个物质（只能显示前10000个）Similarity search 检出如下对Substructure search检出 45739 个物质进行Analyze or Refinea.Analyze第1步使用real-atom attachments （原子分析）第2步使用 stereo （构相）第3步使用 Precision （精密分析）第4步使用 Ring skeletons （环结构分析）b.Refine对结果的801种物质进行限定分析第1步使用chemical structure 将双键限定为E型得到25个物质第2步用Isotope-containing substance去除C、O的放射性同位素得到13个物质第3步使用commercial availability（商业化限定）得到2个物质至此得到了要检索的物质富马酸二甲酯（Demethyl Fumarate）<ii>Molecular formula<略，输入C6H804再同上即可得>C .Explore Reactions检索醚→烯的反应反应步设定为1，检索得到68755个反应假如反应物规定为乙醚，则剩余84反应；如果要获得不是乙醚为反应物的反应，则回到上一步保存一个*.sfr文件，再按上述步骤进行后即可得到9995个不从乙醚出发的反应。

胶原酶IVsigmac5138pis__说明书

Collagenasefrom Clostridium histolyticum Product Number C5138 Storage Temperature −0 °CProduct DescriptionCAS Number: 9001-12-1Molecular Weight: This collagenase obtained from the culture filtrate of Clostridium histolyticum. The culture filtrate that is obtained is thought to contain at least7 different proteases ranging in molecular weight from 68-130 kDa.1Many references exist for using collagenase to digest various tissues. The choice of one technique over another is often arbitrary and based more on past experience than on an understanding of why the method works and what modifications could lead to better results. Concentrations typically vary from0.1 to 5 mg/ml, and digestion time is something that should be experimentally monitored using a very gentle agitation system to check for tissue dissociation. Collagenase treatment can cause some cells to die. Satisfactory efficiency of cell dissociation without causing too much cell death typically is achieved from 15 minutes to several hours, but can fall outside of this range if the concentration is unusual. The preferred buffer to use is Krebs Ringer Buffer with calcium and BSA. Zn2+ is required for activity, but it is tightly bound to the collagenase during purification. Additional Zn2+ should not be necessary as long as no chelator is added to the solution during digestion. When this enzyme is tested for suitability for the release of hepatocytes, the collagenase is used at approximately 1 mg/ml in a total volume of 100 ml for each rat liver.If excessive cell death is observed with concentrations previously used, the new lot used might have a higher specific activity. Lowering the enzyme concentration and/or adding BSA or serum (0.5% and 5-10%, respectively) is recommended. These components are added to stabilize the cells to futher digestion by the enzyme. To sterile filter solutions of collagenase, first centrifuge the solution or filter through a 0.8 µm filter to remove insolubles. This will remove particulates and reduce the probability of clogging the 0.2 µm filter during sterile filtration.Radiolabeled gelatin has been used to measure the activity and mechanism of collagenase digestion.2Mandl units have the same description as Sigma collagen digestion units. The conversion factor for Mandl units/Wuensch units to Sigma units is approximately 1000-2000 to 1.Most of the proteases in this preparation may be inhibited by a combination of benzamidine hydrochloride (Product No. B6506) and TLCK (Product No. T7254) without affecting the activity of collagenase. However, there is no guarantee that all the proteases will be inhibited. For general applications, the working concentrations for benzamidine hydrochloride is about 1 mM and TLCK is 0.100-0.135 mM. If a collagenase with no significant protease activity is required, Product No. C0773 is recommended, since this product has been tested for any residual protease activity and found to contain less than 1 unit of neutral protease per mg protein. Precautions and DisclaimerFor Laboratory Use Only. Not for drug, household or other uses.Preparation InstructionsFor enzymatic activity measurement, an enzyme stock solution is prepared by dissolving 0.05 - 0.1 mg/ml collagenase in 50 mM TES buffer, pH 7.4, (37 °C) containing 0.36 mM calcium chloride. Final concentrations in the reaction mixture are 50 mM TES, 0.36 mM calcium chloride, 25 mg collagen (Product No. C9879), and 0.005-0.01 mg collagenase.Storage/StabilitySolutions at neutral pH and with adequate calcium ion (0.3-0.5 mM) will retain activity for at least 5 hours at 37 °C.Solutions at -20 °C are stable for several months.3 References1. Angleton, E.L., et. al., Preparation andreconstitution with divalent metal ions of class Iand class II Clostridium histolyticumapocollagenases. Biochemistry, 27, 7406-7412(1988).2. Mookhtiar, K.A., et al., Properties of radiolabeledtype I, II, and III collagens related to their use assubstrates in collegenase assays. Anal.Biochem., 158, 322-333 (1986).3. Schomburg, D., et al., Enzyme Handbook,(Springer-Verlag Berlin Heidelberg:1991), pp. 1-6.MWM/NSB 5/06Sigma brand products are sold through Sigma-Aldrich, Inc.Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side ofthe invoice or packing slip.。

EMSAprotocol

EMSAprotocolEMSA PROTOCOL IMPORTANT REAGENT Boitin-N4-CTP (invitrogen # 15918-18) TdT (NEB # M0252L)Poly(dI.dC) (sigma ＃P4929)Hybond-N+ 尼龙膜(Amersham ＃RPN303B) ECL发光液(Perkin Emor)BUFFERBuffer A(store at 4℃)Hepes (pH7.9) 10mMKCl 10mMEDTA 0.1mMDTT(fresh added) 1mMPMSF(fresh added) 0.5mMBuffer B (store at 4℃)0.5M EDTA in PBS (pH7.4)Buffer C(store at 4℃)Hepes (pH7.9) 20mMNaCl 0.4MEDTA 1mMDTT(fresh added) 1mMPMSF(fresh added) 1MmBuffer I (store at RT)Tris (pH7.5) 0.1MNaCl 1MMgCl2 2mMBuffer II (pH7.5) (store at RT)马来酸100mMNaCl 150mMBuffer III (store at RT)Tris(pH9.5) 100mMNaCl 100mMMgCl2 50mMBlocking Buffer (store at 4℃)0.3％Tween-20, 0.3%Triton X-100, 5%BSA in Buffer IWash Buffer0.3％Tween-20 in Buffer II20×SSC (1L pH7.0)NaCl 175.3g柠檬酸钠88.2gBinding Buffer (store at -20℃)1×10×25mM HEPES (pH7.4) 250mM HEPES (pH7.4) 50mM KCl 500mM KCl5mM MgCl2 50mM MgCl20.5mM EDTA 5mM EDTA1mM DTT 10mM DTT5% Glycerol 50% 0 Glycerol 10mg/ml BSA (TE, pH8.0, store at -20℃)Poly(dI.dC) (store at -20℃)SA-HRP(储存液store at -20℃工作液store at 4℃)1mg/ml in 50%PBS (pH7.2) 50%Glycerin5×TBE (1L)Tris 54g硼酸27.5gEDTA 3.72g 20ml 0.5mol/L EDTA（pH8.0）5×TdT Reaction Buffer (store at -20℃) (pH7.2)Sodium cacodylate 0.5MCoCl210mMTCEP 1mMBiotin-N4-CTP (store at -20℃)Biotin-N4-CTP 50mMTris (pH7.5) 10mMEDTA 1mMTE buffer10mM Tris，1mM EDTA，pH8.0PROTOCOL生物素标记探针1 按如下顺序加入反应物，温和混匀，切勿涡漩超纯水25μl5×TdT Reaction Buffer 10μl探针(1μM) 5μlBiotin-N4-CTP 5μlTdT (2U/μl) 5μl37℃反应30分钟2 加入2.5μl 0.2M EDTA终止反应3加入50μl 酚：氯仿，短暂涡漩，15000g离心2min，保留上层水相，－20℃保存。

GC-MS法测定甲磺酸中3种甲磺酸烷基酯类遗传毒性杂质_NormalPdf

学报Journal of China Pharmaceutical University2020，51（4）：472-478472GC-MS法测定甲磺酸中3种甲磺酸烷基酯类遗传毒性杂质陈忆铃1，冯江江2，杨海雪3，时雅萍1，李龙囡2，冯芳1*（1中国药科大学药物分析系，南京211198；2无锡市药品检验所，无锡214028；3扬子江药业集团南京海陵药业有限公司，南京210049）摘要建立一种液液萃取GC-MS分析方法，用于测定甲磺酸中遗传毒性杂质甲磺酸甲酯、甲磺酸乙酯、甲磺酸异丙酯。

采用甲基聚硅氧烷毛细管色谱柱，程序升温，进样口温度220℃，不分流高压进样；质谱正离子模式，选择性监测m/z56、m/z 79、m/z80及m/z123离子。

实验结果显示，甲磺酸甲酯、甲磺酸乙酯、甲磺酸异丙酯对应色谱峰之间实现了基线分离，空白提取溶液无干扰；3种遗传毒性杂质在37~1480ng/mL范围内线性关系良好，且不同浓度水平平均回收率分别为104.99%、107.26%及108.85%，RSD均不超过4.54%。

该方法具有专属、灵敏、准确、稳定、通用性好的特点，已用于多种不同来源市售甲磺酸中甲磺酸烷基酯类杂质的检测和控制。

关键词甲磺酸；甲磺酸烷基酯；遗传毒性杂质；液液萃取；GC-MS法；含量测定中图分类号R917文献标志码A文章编号1000-5048（2020）04-0472-07doi：10.11665/j.issn.1000-5048.20200413引用本文陈忆铃，冯江江，杨海雪，等.GC-MS法测定甲磺酸中3种甲磺酸烷基酯类遗传毒性杂质［J］.中国药科大学学报，2020，51（4）：472–478.Cite this article as：CHEN Yiling，FENG Jiangjiang，YANG Haixue，et al.Determination of genotoxic impurities of alkyl methanesulfonates in methanesulfonic acid by gas chromatography–mass spectrometry［J］.J China Pharm Univ，2020，51（4）：472–478.Determination of genotoxic impurities of alkyl methanesulfonates in meth⁃anesulfonic acid by gas chromatography–mass spectrometryCHEN Yiling1,FENG Jiangjiang2,YANG Haixue3,SHI Yaping1,LI Longnan2,FENG Fang1*1Department of Pharmaceutical Analysis,China Pharmaceutical University,Nanjing211198;2Wuxi Center for Drug Safety Control, Wuxi214028;3Yangtze River Pharmaceutical Group Nanjing Hailing Pharmaceutical Co.,Ltd.,Nanjing210049,ChinaAbstract An analytical liquid-liquid extraction-gas chromatography–mass spectrometry(LLE-GC-MS)method was established for the determination of genotoxic impurities including methyl methanesulfonate(MMS),ethyl methanesulfonate(EMS)and isopropyl methanesulfonate(IMS)in methanesulfonic acid.An Agilent HP-1MS capillary column(30m×0.32m,1µm)was used for separating the analytes by programmed heating with the inlet temperature of220°C.Mass spectrometry was operated in positive ion mode,and selective ion monitors were set at m/z80for MMS,m/z79for EMS,m/z123for IMS and m/z56for internal standard butyl methanesulfonate(BMS).Results showed that the baseline separation of MMS,EMS and IMS was achieved,and the blank extraction solution had no interference;good linearity was achieved in the range of37-1480ng/mL for three alkyl methanesulfonates;The mean recoveries of MMS,EMS,IMS were104.99%,107.26%,108.85%, respectively,with RSD≤4.54%.The established method has the characteristics of good specific,sensitive, accurate,stable and versatility,and has been used for the detection and control of alkyl methanesulfonate impurities in methanesulfonic acid from a variety of manufacturers.Key words alkyl methanesulfonates;genotoxic impurities;methanesulfonic acid;liquid-liquid extraction;GC-MS;determination of content收稿日期2020-05-08*通信作者Tel：139********E-mail：fengfang@基金项目国家药典委员会药品标准制修订研究课题资助（No.ZG2018-4-03）第51卷第4期陈忆铃，等：GC-MS法测定甲磺酸中3种甲磺酸烷基酯类遗传毒性杂质This study was supported by Chinese Pharmacopoeia Commission on the Research and Revision of Drug Standards(No.ZG2018-4-03)甲磺酸甲酯、甲磺酸乙酯、甲磺酸异丙酯是DNA反应活性物质，在极低水平下仍存在致突变/致癌风险［1-4］，为此，全球药品监督管理部门高度重视，纷纷要求相关企业在药物研发、生产过程中对其严格控制［5-7］，国家药典委员会在即将执行的2020版《中华人民共和国药典》中新增了“遗传毒性杂质控制指导原则”［8］。

Aptima 组合2检测试纸与其他生物标志物检测说明书

Collection Device
ThinPrep Pap Test Aptima Multitest Swab, Aptima Urine
Aptima Unisex Swab ThinPrep Pap Test Aptima Multitest Swab Aptima Multitest Swab Aptima Multitest Swab
Trichomonas vaginalis NAAT Chlamydia trachomatis / Neisseria gonorrhoeae / Trichomonas vaginalis NAAT Herpes Simplex Virus 1 & 2 NAAT
Test Code
LAB491 87491.11 LAB502323 LAB1093 LAB1092 LAB1091 LAB2097
Sample Type
Cervicovaginal Vaginal, Throat, Rectal, Urine Conjunctiva, Male Urethral, Endocervical
Cervicovaginal Vaginal Vaginal
Anogential lesion
8/31/2021
8/31/2021
Bellin Health Test Order Codes
Test Description
Image-Guided Pap Test Image-Guided Pap Test with reflex to Aptima HPV when ASCUS Image-Guided Pap Test with reflex to Aptima HPV when ASCUS or Negative Image-Guided Pap Test and Aptima HPV Aptima HPV Genotype 16, 18/45 Cytology Non-Gynecological