高效液相色谱法测定心可舒片中丹参素钠和原儿茶醛含量

高效液相色谱法测定心可舒片中丹参素钠和原儿茶醛含量
屈蓉;仇雅静;陈骁鹏;叶慧
【期刊名称】《中国药业》
【年(卷),期】2012(021)019
【摘要】目的建立心可舒片中有效成分丹参素钠和原儿茶醛的含量测定方法.方法采用Waters SymmetryC18柱(150mm × 3.9 mm,3.5 μm),以甲醇-0.5％醋酸溶液(10:90)为流动相,检测波长为280 nm,流速为1.0 mL/min.结果丹参素钠质量浓度在50.06～250.30μg/mL范围内与峰面积呈良好线性关系,r=0.999 1,方法平均
回收率为100.61％,RSD=1.23％(n=5);原儿茶醛质量浓度在5.28～47.52 μg/mL
范围内与峰面积呈良好线性关系,r=0.999 5,方法平均回收率为99.84％,RSD=1.69％(n=5).结论 15批样品测定结果表明,该方法简便、准确,可用于心可舒片中丹参素
钠和原儿茶醛的含量测定,为该药的质量评价提供了依据.%Objective To establish a method for simultaneous determination of the contents of sodium danshensu and protocatechuic aldehyde in Xinkeshu Tablets. Methods The HPLC method was carried out with the Waters Symmetry C18 column and methanol -0.5％ acetic acid(10:90) as the mobile phase. The flow rate was 1.0mL/min with the detective wavelength of 280 nm. Results The calibration curve of sodium danshensu as linear within the range of 50. 06 - 250. 30 μg/mL( r = 0. 999 1), the average recovery rate was 100.61％, RSD was 1. 23％ (n =5). The calibration curve of protocatechuic aldehyde was linear within the range of 5. 28-47. 52 μg/mL (r =0. 999 5), the average recovery rate was 99.84％, RSD was 1. 69％ ( n = 5). Conclusion The
detection Results in 15 batches of samples show that this method is easy to operate, accurate, and can be used to determine the contents of sodium danshensu and protocatechuic aldehyde in Xinkeshu Tablets, and provides the basis for the quality evaluation of Xinkeshu Tablets.
【总页数】2页(P23-24)
【作者】屈蓉;仇雅静;陈骁鹏;叶慧
【作者单位】江苏省泰州市食品药品检验所,江苏泰州 225300;江苏省泰州市食品药品检验所,江苏泰州 225300;江苏省泰州市食品药品检验所,江苏泰州 225300;江苏省泰州市食品药品检验所,江苏泰州 225300
【正文语种】中文
【中图分类】R284.1;R286.0
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