Hall, J.K et al., (2005)

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国际小学科学教育的发展趋势

国际小学科学教育的发展趋势

国际小学科学教育的发展趋势丁邦平作者工作单位:北京师大国际与比较教育研究所——兼谈我国小学自然课的若干问题一、小学科学教育的发展历程在西方,19世纪初,裴士泰洛齐就倡导在小学进行实物教学(obje-ct teaching)。

随着裴士泰洛齐教育思想19世纪中期在欧美各国逐渐传播,以及初等义务教育在这些国家的实施,实物教学形态的科学启蒙教育开始在工业化国家得到推广。

例如,19世纪50年代,实物教学的思想传到美国后,在全国教育协会发起和支持的澳滋威格计划(Oswego Pian)推动下,美国小学也盛行实物教学。

实物教学的主要作法是教儿童详细描述各种动物、植物和矿物,使其观察和学习自然现象。

通过实物教学,希望儿童学会观察和交流——两种“研究”科学的基本能力。

但在当时的实际教学过程中,由于绝大多数小学教师缺乏科学训练,实物教学多为对实物的机械描述和记忆。

到19世纪末期,这种实物教学法在美国便不再流行了。

19世纪90年代至20世纪头10年期间,美国教育开始摆脱欧洲教育传统的影响,进入教育理论和实践的创新时期。

当时,美国著名心理学家霍尔(S.Hall)的儿童研究和杜威的实用主义教育思想开始对美国教育产生影响。

此后小学科学教育发生了较大的变化。

首先是学科名称上的改变,即以“自然学习”(nature study)取代了实物教学。

其次是教学内容上的变化,这个时期科学启蒙教育注重儿童自身的需要,因而健康和卫生加入到小学科学教育内容中。

第三,强调科学方法的训练。

杜威认为,科学方法的训练与获得实际科学知识至少同样重要。

在奉行杜威实用主义教育哲学的学校里,科学教学还注重解决实际问题。

第四,这一时期美国科学教育研究工作在一些大学里开展起来,如1927年哥伦比亚大学克雷格(Gerald S.Craig)的博士论文被公认为小学科学教育发展中里程碑式著作。

(注:Esler,W.K.et al.(1993),Teaching Elementary,Science,Belmont,Califor nia:Wadsworth Publishing Company,pp.9-10.)克雷格指出了科学在卫生和安全等方面的实用价值,并认为科学对于一个公民的普通教育是至关重要的。

跨文化交际理论主要参考书目

跨文化交际理论主要参考书目

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Pluralism:the Philosophy and Politics of Diversity, New York: Routledge, 2000.Ball, T. “From paradigms to research Program: Toward a post Kuhnian political science”, in H. B. Asher at al. (eds.) Theory Building and Data Analysis in the Social Sciences, Knoxville: The University of Tennessee Press, 1984, pp. 23-43. Beiner, R. et al. (eds.). Canadian Political Philosophy, New York: Oxford University Press, 2001.Benet-Martinez, V. et al. “Biculturalism and cognitive complexity: Expertise in cultural representations”, in Journal of Cross-Cultural Psychology, 2006, 37, pp. 368-407.Bennett, M. “T owards ethnorelativism: A development model of intercultural sensitivity”, in M. Paige (ed.).Education for the Intercultural Experience (second edition) , Yarmouth: Intercultural Press, 1986.Burgoon, J. K. “Interpersonal expectancy violations, and emotional communication”, in Journal of Language and Social Psychology, 1993, 12, pp. 30-48.Burgoon, J. 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J. “I ntercultural communication competence: A synthesis”, in Communication Yearbook, 19, 1996, pp. 353-383.Chen, G. M. &Starocta, W. J. Foundations of Intercultural Communication, Boston: Allyn & Bacon, 1998.Chen, G. M. &Starocta, W. J. (eds). Communication and Global Society, New York: Peter Lang, 2000.Chen, G. M. “Toward transcultural understanding: A harmony theory of Chinese communication”, in V. H. Milhouse et al.(eds). Transcultural Realities: Interdisciplinary Perspectives on Cross-cultural Relations, 2001, Thousand Oaks: Sage Publications, pp.55-70. Chen, G. M. “A model of global communication competence”, in China Media Research, 2005, 1, pp. 3-11.Chen, G. M. “Asian communication studies: What and where to now”, in The Review of Communication, 2006, 6, pp. 295-311. Chen, L. “Interaction involvement and partners of topical talk: A comparison of intercultural and intracultural dyads”, in International Journal of Intercultural Relations, 19, 1995, pp.463-482. 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Readings in Cultural Contexts, Mountain View: Mayfeild Publishing Company, 2002. Glazer, N. et al. (eds.). Ethnicity: Theory and Experience, Cambridge: Harvard University Press, 1975.Goffman, E. Interaction Ritual: Essays on Face-to-Face Behavior, New York: Pantheon Books, 1967.Gorton, M. M. Assimilation in American Life: The Role of Race, Religion, and National Origion, New York: Oxford University Press, 1964.Grace, G. W. The Linguistic Construction of Reality, New York: Groom Helm, 1987. Gudykunst, W. B. (ed.).Intercultural Communication Theory: Current Perspectives, Beverly Hills, Sage Publications, 1983. Gudykunst, W. B. “Anxiety/ uncertainty management(AUM) theory”in R. L. Wiseman(ed.). Intercultural Communication Theory,Thousand Oaks: Sage Publications, Inc., 1995, pp.8-58.Gudykunst, W. B. “Applying anxiety/ uncertainty management(AUM) theory to intercultural adjustment training”, in International Journal of Intercultural Relations, 1998, 22, pp.227-250. Gudykunst, W. B.& Nishida, T. “Anxiety, uncertainty, and perceiced effectiveness of communication across relationships andcultures”, in International Journal of Intercultural Relations, 2001, 25, pp.55-71.Gudykunst, W. B.& Mody, B. (eds). Handbook of International and Intercultural Communication (second edition), Thousand Oaks: Sage Publications, Inc., 2002. Gudykunst, W. B. (ed.). Cross-Cultural and Intercultural Communication, Thousand Oaks: Sage Publications,2003.Gudykunst, W. B. &Kim, Y. Y. Communicating with Strangers: An Approach to Intercultural Communication (forth edition), New York: The McGraw-Hill Companies, Inc., 2003.Gudykunst, W. B. (ed.). Theorizing about Intercultural Communication, Thousand Oaks: Sage Publications, Inc.,2005.Guibernau, M. “National identity and modernity”, in A.Dieckoff& N. Gutierrez(eds.). Modern Roots: Studies of National Identity, Aldershot: AshgatePublishing Limited, 2001.Gullahorn, J. T. & Gullahorn J. 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Conflict Thought: Selfhood as Creative Transformation, New York: State University of New York Press, 1985. Vago, J. M. (ed.). Culture Bound, Cambridge: Cambridge University Press, 1986. Ward, C. “Thinking outside Berry boxes: New perspectives on identity, acculturation and intercultural relations”, in International Journal of Intercultural Relations, 32, 2008, pp. 105-114.White, S. K. (ed.). The Cambridge Companion to Habermas, Beijing: SDX Joint Publishing Company, 2006.Willett, C. (ed.). Theorizing Multiculturalism: A Guide to the Current Debate, Malden: Blackwell Publishers Ltd., 1998.Williams, R. Keywords: A Vocabulary of Culture and Society, London: Fontana Press, 1983.Wiseman, R. L. (ed.).Intercultural Communication Theory, Thousand Oaks: Sage Publications, Inc.,1995.Wood, J. T. Communication Theories in Action: An Introduction (second edition), Belmont: Wadsworth, 2000.Worchel, S. “Culture’s role in conflict and conflict management: Some suggestions, many questions”, in International Journal of Intercultural Relations, 2005, 29, pp. 739-757.Xiao, X. “Zhong (centrality): Aneverlasting subject of Chinese discourse”, in Intercultural Communication Studies, 2003, 12, pp. 137-149.Yang, R. P. et al. “Multiple routes to cross-cultural adaptation for international students: Mapping the paths between self-construals, English language confidence, and adjustment”, in International Journal of Intercultural Relations, 2006, 30, pp. 478-506.Zilles, A. M. S. &King, K. “Self-presentation in sociolinguistic interviews: Identities and language variation in Panambi”, in Sociolinguistics, 2005, 9, pp. 74-75.中文参考书目【英】安德鲁·埃德加著,杨礼根与朱松峰译:《哈贝马斯:关键概念》,南京:江苏人民出版社,2009年版。

全皮下埋藏式心脏转复除颤器

全皮下埋藏式心脏转复除颤器

全皮下埋藏式心脏转复除颤器:安全有效的心脏除颤器摘要:全皮下埋藏式心脏转复除颤器(Subcutaneous implantable cardioverter defibrillator,S—ICD)目前已在欧洲、新西兰、美国投入临床使用。

S-ICD主要由脉冲发生器、电极导线组成,与经静脉植入型心律转复除颤器(Transvenous implantable cardioverter defibrillator,TV—ICD)不同的是,S—ICD的脉冲发生器经皮下置于左侧胸壁,电极是由两个感知电极及一个电击线圈组成的,经皮下置于胸骨旁。

作为全皮下心脏除颤系统,S-ICD可在一定程度上避免与静脉导线相关的围手术期及长期并发症,且手术过程不需要在透视下进行.S-ICD的适应人群主要包括伴有静脉畸形或梗阻不适合经静脉行心脏转复除颤器的儿童、感染风险大以及有猝死风险的年轻心律失常患者(如Brugada综合症、长QT综合症及肥厚性心肌病)。

然而,由于缺乏腔内电极,S—ICD无起搏功能(除电击后短期起搏外),因此,I-SCD不适合缓慢性心律失常患者及有心脏再同步化治疗(Cardiac resynchronization therapy,CRT)适应症的患者.引言埋藏式心律转复除颤器(Implantable cardioverter—defibrillator,ICD)目前是心源性猝死的一级预防[1,2]及二级预防[3]的有效手段。

迄今为止,大部分ICD植入系统均通过传统植入方式,即经胸腔内大静脉将除颤电极置于右室,然而,TV—ICD具有较高的围手术期及远期并发症.最近的一项大规模研究表明,TV—ICD主要并发症的发生率大约为1。

5%,这些主要并发症包括:院内死亡、心脏骤停、心脏穿孔、心脏瓣膜损伤、冠状静脉损伤、血胸、气胸、深静脉炎、短暂性脑缺血发作、中风、心肌梗死、心包填塞以及动静脉瘘[4]。

同时,随着时间的推移,电极绝缘故障导致的电极故障的发生率日益增加,有研究显示,电极植入10年后电极故障的发生率约为20%[5].因此,尽管TV—ICD能够有效治疗快速性室性心律失常,它的缺点也是显而易见的。

植物内生菌的研究与应用

植物内生菌的研究与应用

植物内生菌的研究与应用目录第一章植物内生菌的研究 (3)第二章分离过内生菌的植物种类 (17)第三章植物内生菌种类 (122)第三章相关作物内生菌的研究 (165)第四章植物内生菌研究参考文献 (166)第一章植物内生菌的研究1 内生菌的发现和定义植物体内普遍存在着内生菌,但是由于这些内生菌生活在没有外在感染症状的健康植物组织内部,所以内生菌的存在和作用长期以来被忽视。

直到20世纪30年代,发现造成畜牧业重大损失是由于牲畜食了感染内生真菌的牧草,这才开始对内生菌的深入研究(Leuchtmann A 1992)。

1886年,德国科学家Barry首先提出了内生菌一词“endophyte”(李能章彭远义2004)。

Carrol在1986年将内生菌阐述为生活在地上部分、活的植物组织内不引起明显症状的微生物(何劲刘蕴哲康冀川2006)。

1991年,Petrini提出内生菌是指生活史的一定阶段生活在活体植物组织内不引起植物明显病害的微生物(Petrini O 1986)。

1992年Kleopper等认为植物内生菌是指能够定殖在植物细胞间隙或细胞内,并与寄主植物建立和谐联合关系的一类微生物,并首次提出“植物内生细菌”的概念,他认为能在植物体内定殖的致病菌和菌根菌不属于内生菌(何红邱思鑫胡方平关雄2004)。

1997年Hallmann对植物内生细菌的概念进行补充,认为植物内生细菌是从表面消毒组织中分离得到或从植物内生细菌是从表面消毒的植物组织中分离得到或从植物内部汁液获得的,并对植物表观上无危害及明显症状的,但它们的存在并未使植物的表型特征和功能有任何改变的细菌(Hallmann J Kleopper J W Rodriguez-Kabana R 1997)。

目前,较被公认的定义为:指那些在其生活史的一定阶段或者全部阶段生活于健康植物的各种组织和器官内部的真菌或者细菌,被感染的宿主植物(至少是暂时)不表现出外在症状。

地铁车站PM2.5和PM10颗粒物浓度实测及分析

地铁车站PM2.5和PM10颗粒物浓度实测及分析

地铁车站 PM2.5 和 PM10颗粒物浓度实测及分析刘文龙 何垒 李懋中铁二院华东勘察设计有限责任公司摘 要: 为了进一步了解地铁车站内环境中的颗粒物浓度分布情况, 在 2015 年 11 月对上海市A 、 B 两个地铁车 站进行了实地监测,分析了PM2.5和PM10颗粒物浓度在一天中的变化规律及其影响因素。

测试结果显示站厅公 共区,站台公共区与轨行区的PM2.5浓度在监测时段内逐时变化规律相似。

站厅公共区,站台公共区PM10与 PM2.5在监测时段内逐时变化规律相似。

地铁车站站台内PM2.5/PM10质量浓度比值平均值为0.65~0.93, 颗粒物 污染主要为细颗粒物。

关键词: 站厅 站台 轨行区 PM2.5PM10 监测Site Monitoring and Analysis of Concentrationof PM2.5and PM10in Subway StationLIU Wen­long,HE Lei,LI MaoCREEC East China Survey and Design Co.,Ltd.Abstract: In order to get a better understanding of the Particle Concentration in subway station,in November 2015,two subway stations in Shanghai were monitored,and the changes of the concentration of PM2.5and PM10during the day and its influencing factors was analyzed.The results show that the changes of PM2.5concentration is similar at the public area of the station hall,the platform public area and the railroad area hour by hour;the changes of PM10and PM2.5at the public area of the station hall and the platform public area is similar hour by hour;the average mass concentration ratio of PM2.5/PM10in subway station platforms is 0.65~0.93,and the main particulate contaminant is fine particle.Keywords:station hall,platform,track area,PM2.5,PM10,monitoring收稿日期: 2020­10­10作者简介: 刘文龙 (1988~), 男, 硕士, 工程师; 浙江省杭州市江干区三里亭路57号 (310004); E­mail:*********************0 引言PM2.5和PM10的浓度是影响地铁站站厅站台空气品质的主要参数之一, 有研究表明地铁车站内空气环境中所含的颗粒物与其他场合相比有较大区别 [1­2]。

布托啡诺复合舒芬太尼在乳腺癌根治术后静脉镇痛中的应用_骆喜宝

布托啡诺复合舒芬太尼在乳腺癌根治术后静脉镇痛中的应用_骆喜宝

如芬太尼、舒芬太尼等历来占主导地位。 随着剂量 的增加,各种程度的疼痛能得到有效的控制, 静脉 自控镇痛效果确切,但其副作用多,不良反应发生 率高[3]。 近年来,多模式镇痛受到重视,采用不同镇 痛方案或不同镇痛药物作用的相加和协同,可以达 到充分的镇痛,同时因药物剂量的降低而使不良反 应 减 少 [4]。
2h 4.7 ± 1.4 1.6 ± 1.5* 1.5 ± 1.6*
4h 4.5 ± 1.3 1.5 ± 1.7* 1.7 ± 1.9*
8h 4.5 ± 1.8 1.9 ± 1.4* 2.0 ± 1.3*
12 h 4.6 ± 1. 4 1. Nhomakorabea ± 1.3* 1.8 ± 1.3*
24 h 4.5 ± 1.9 1.7 ± 1.6* 1.9 ± 1.5*
关键词 乳腺肿瘤; 布托啡诺; 舒芬太尼; 静脉镇痛
布托啡诺是一种典型的阿片受体兴奋-拮抗型 止痛药,镇痛效价是吗啡的 5 ~ 8 倍,而呼吸抑制仅 仅为吗啡的 1 / 5[1]。 舒芬太尼镇痛机制与吗啡相似, 为 μ 阿片受体激动剂,术后镇痛作用较强,安全范 围远远大于吗啡[2]。 本研究观察布托啡诺复合舒芬 太尼在乳腺癌改良根治手术后静脉镇痛中的效能和 不良反应,以寻求一种安全、有效的术后镇痛方法。 1 资料与方法 1.1 一般资料 选 择 我 院 2009 年 1 月 至 2010 年 4 月全麻下择期行乳腺癌根治术患者 63 例, 年龄 38 ~ 68 岁,体重 45 ~ 65 kg,ASA Ⅰ~Ⅱ 级;无阿片 药物滥用史,无高血压及肝肾功能异常,无酗酒史; 所有受试者经医院伦理委员会同意并签署同意书 后进入研究。 63 例乳腺癌改良根治手术患者分为 A、B、C 组,3 组患者年龄、身高、体重、手术时间、麻 醉时间等差异均无统计学意义,见表 1。 1.2 麻醉方法 麻醉前肌注苯巴比妥钠 0.1 g、阿 托品 0.5 mg。 以咪达唑仑 0.05 ~ 0.1 mg / kg、丙泊酚 1 ~ 2 mg / kg、芬 太 尼 4 μg / kg、阿 曲 库 铵 0.5 mg / kg 诱导插管,术中丙泊酚维持 4 ~ 8 mg / (kg·h),异 氟 醚吸入维持麻醉。 1.3 镇痛方法 A 组:酒石酸布托啡诺 4 mg(江苏 恒瑞医药股份有限公司生产, 规格:1 mL;1 mg)加 生理盐水共 100 mL。 B 组:舒芬太尼 100 μg (宜昌 人福药业有限公司生产,规格:1 mL;50 μg)加生理 盐水共 100 mL。C 组:酒石酸布托啡诺 4 mg 复合舒 芬 太 尼 50 μg 加 生 理 盐 水 共 100 mL。 术 毕 清 醒 拔 管 后 接 静 脉 镇 痛 泵 行 静 脉 术 后 镇 痛 (PCIA)。 持 续

新视野大学英语读写教程(第二版)第二册课文及翻译

新视野大学英语读写教程(第二版)第二册课文及翻译

Unit 1Time-Conscious AmericansAmericans believe no one stands still. If you are not moving ahead, you are falling behind. This attitude results in a nation of people committed to researching, experimenting and exploring. Time is one of the two elements that Americans save carefully, the other being labor."We are slaves to nothing but the clock," it has been said. Time is treated as if it were something almost real. We budget it, save it, waste it, steal it, kill it, cut it, account for it; we also charge for it. It is a precious resource. Many people have a rather acute sense of the shortness of each lifetime. Once the sands have run out of a person's hourglass, they cannot be replaced. We want every minute to count.A foreigner's first impression of the US is likely to be that everyone is in a rush—often under pressure. City people always appear to be hurrying to get where they are going, restlessly seeking attention in a store, or elbowing others as they try to complete their shopping. Racing through daytime meals is part of the pace of life in this country. Working time is considered precious. Others in public eating-places are waiting for you to finish so they, too, can be served and get back to work within the time allowed. You also find drivers will be abrupt and people will push past you. You will miss smiles, brief conversations, and small exchanges with strangers. Don't take it personally. This is because people value time highly, and they resent someone else "wasting" it beyond a certain appropriate point.Many new arrivals in the States will miss the opening exchanges of a business call, for example. They will miss the ritual interaction that goes with a welcoming cup of tea or coffee that may be a convention in their own country. They may miss leisurely business chats in a restaurant or coffee house. Normally, Americans do not assess their visitors in such relaxed surroundings over extended small talk; much less do they take them out for dinner, or around on the golf course while they develop a sense of trust. Since we generally assess and probe professionally rather than socially, we start talking business very quickly. Time is, therefore, always ticking in our inner ear.Consequently, we work hard at the task of saving time. We produce a steady flow of labor-saving devices; we communicate rapidly through faxes, phone calls or emails rather than through personal contacts, which though pleasant, take longer—especially given our traffic-filled streets. We, therefore, save most personal visiting for after-work hours or for social weekend gatherings.To us the impersonality of electronic communication has little or no relation to the significance of the matter at hand. In some countries no major business is conducted without eye contact, requiring face-to-face conversation. In America, too, a final agreement will normally be signed in person. However, people are meeting increasingly on television screens, conducting "teleconferences" to settle problems not only in this country but also—by satellite—internationally.The US is definitely a telephone country. Almost everyone uses the telephone to conduct business, to chat with friends, to make or break social appointments, to say "Thank you", to shop and to obtain all kinds of information. Telephones save the feet and endless amounts of time. This is due partly to the fact that the telephone service is superb here, whereas the postal service is less efficient.Some new arrivals will come from cultures where it is considered impolite to work too quickly. Unless a certain amount of time is allowed to elapse, it seems in their eyes as if the task being considered were insignificant, not worthy of proper respect. Assignments are, consequently, given added weight by the passage of time. In the US, however, it is taken as a sign of skillfulness or being competent to solve a problem, or fulfill a job successfully, with speed. Usually, the more important a task is, the more capital, energy, and attention will be poured into it in order to "get it moving".美国人认为没有人能停止不前。

magneticcarriers:磁性载体

magneticcarriers:磁性载体

immobilized on magnetic carriers.Proceedings of European Congress of Chemical Engineering (ECCE-6)Copenhagen, 16-20 September 2007Application of magnetic nanostructures in biotechnological processes: Biodiesel production using lipase immobilized on magnetic carriersK. J. Dussán,a O. H. Giraldo,b C. A. Cardona,aa Department of Chemical Engineering, National University of Colombia at Manizales, Cra. 27 No. 64-60, Of. F-505, Manizales, Colombiab Department of Physics and Chemistry, National University of Colombia at Manizales, Cra. 27 No.64-60, Manizales, ColombiaAbstractMagnetic nanostructures have gained a remarkable interest in the last years both for basic research and applied studies. The use of magnetic nanostructures has been proven in biochemistry, biomedicine, and waste treatment among other fields. This broad range of applications is based on the fact that magnetic particles have very large magnetic moments, which allow them to be transported and driven by external magnetic fields. The magnetic nanostructures have also a great potential in biotechnological processes taking into account that they can be utilized as a carrier for enzymes during different biocatalytic transformations. In this way, the biocatalyst can be easily manipulated by a controlled magnetic field allowing it to be located permanently in the zone where the maximum concentration of reagents is present. In this work, some applications are presented. Particularly, the system composed of an immobilized lipase on a magnetic nanostructure for biodiesel production is analyzed. This system makes possible the intensification of the process due to the accomplishment of a reaction-extraction enzymatic process favoring the separation of the products formed during the transesterification reaction. In addition, the magnetic nature of the carrier permits the preferential location of the biocatalyst in the separation surface between the two liquid immiscible phases present in the system. Finally, techniques for the immobilization of different enzymes on magnetic carriers are described. This technology can offer innovative configurations allowing the intensification of enzymatic processes and the reduction of their costs.Keywords: Biodiesel, reaction-extraction process, magnetic nanostructure, lipase.1.IntroductionMagnetic particles (microspheres, nanospheres and ferrofluids) are widely studied for their applications in various fields in biology and medicine such as enzyme and protein immobilization, genes, radiopharmaceuticals, magnetic resonance imaging, diagnostics, immunoassays, RNA and DNA purification, magnetic cell separation and purification, magnetically controlled transport of anti-cancer drugs as well as hyperthermia generation (Ma, Zhang et al., 2003).Applications in biotechnology impose strict requirements on the particles physical, chemical and pharmacological properties, including chemical composition, granulometric uniformity, crystal structure, magnetic behavior, surface structure, adsorption properties, solubility and low own toxicity. The following parameters of the nanomagnets are critical: (a) particle size (small as possible to improve tissular diffusion and to have long sedimentation times and high effective surface areas), (b) surface characteristics (easy encapsulation of the magnetic nanoparticles protects them from degradation and endows biocompatibility), and (c) good magnetic response (possibility of decreasing nanomagnets concentration in blood and therefore diminishing the associated side effects) (Tartaj, Morales et al., 2005).On the other hand, the use of biocatalysts in transforming fats, oils, partial glycerides and fatty acids into higer-value-added derivates is well documented (Hsu, Jones et al., 2002). One area of interest is the utilization of immobilized lipases for catalyzing the synthesis of simple esters of vegetable oils (Clark, Wagner et al., 1984; Selmi and Thomas, 1998; Shimada, Watanabe et al., 1999) and other agricultural lipid feedstocks (Nelson, Foglia et al., 1996; Foglia, Nelson et al., 1997; Hsu, Jones et al., 2001) (Scheme 1).Some efforts have been made on the immobilization of lipase on the surface of ferromagnetic nanoparticles modified by polymer such as poly(ethylene glycol) and its copolymer with maleic acid (Tamaura, Takahashi et al., 1986; Mihama, Yoshimoto et al., 1988) and in magnetic sol-gel matrices (Kuncová and Sivel, 1997; Reetz, Zonta et al., 1998; Chen and Lin, 2003; Zeng, Luo et al., 2006). The former immobilization method seems to be more attractive because using magnetic nanoparticles as support not only yields a sufficiently large specific surface area for enzyme binding but also has no pore-diffusion resistance and fouling problem. Therefore, the system composed of an immobilized lipase on a magnetic nanostructure can be applicable in reaction-extraction processes. Because the magnetic nature of the carrier permits the preferential location of the biocatalyst in the separation surface between the two liquid immiscible phases present in the system and an easy recover the biocatalysts of medium.In the process reaction-extraction is used the immiscibility of liquid phase that can give naturally inside the reaction system or can be introduce with the addition of solvents (Samant, 1998), achieving the selective separation of intermediate compounds or products, prevent their later reaction. This in-situ separation carry to a reactive reconcentration which deceive (when are had reversible reactions) reach higher conversions. Besides the synergic effect reached by the reaction a separation combination, has the advantage of carry out the process in single equipment.immobilized on magnetic carriers.Scheme 1. Immobilized lipase-catalysed transesterification of triacylglycerols and etherification of fatty acids to simple alkyl esters (Hsu, Jones et al., 2002).2.Experimental2.1 Synthesis of magnetite nanoparticlesMagnetite was made according to the method of Zeng (Zeng, Luo et al., 2006). For the preparation of magnetic carriers, a mixture of FeCl2 (0.2 mol/l) and FeCl3 (0.3 mol/l) aqueous solution was added to a flask that containing stearic acid. Then the mixture was stirred vigorously for a while, and NaOH aqueous solution (4 mol/l) was dropped into the flask. A black precipitate was obtained. The precipitate was filtrated, washed and dried.2.2 Silane-coated magnetite nanoparticlesCertain amount of Fe3O4 (magnetic particle) was suspended in distilled water. A mixture of [3-(2-aminoethylamino)propyl]trimethoxysilane (APTS), methanol and NaF (1%) aqueous solution was stirring for 10 minutes. Then tetraethyl orthosilicate was dropped slowly into the flask and stirred vigorously at room temperature for 24 hours. The precipitate was collected, washed and dried.2.3 Lipase- immobilizedFor the immobilization of the enzyme, glutaraldehyde was added to certain amount of particles, and stirred at room temperature. Then, certain amount of lipase Candida Rugosa was added to phosphate buffer solution and stirred until all the lipase was dissolved. To this solution was added the solution prepared previously, and stirred for several minutes at room temperature. The immobilized lipase was separated bycentrifugation and washed with phosphate buffer and dried. All portions of centrifugation were retained for the determination of protein concentration.3. Results and discussion3.1 Uncoated and coated nanoparticles magnetic According to the XRD pattern (figure 1), the size of particle can be calculated in the following equation (Moore and Reynolds, 1997),θβλcos ··K L = (1) Where L is the mean diameter of particle, λ is the wavelength of copper anode (λ = 1.540562 Ǻ), K is a constant (K = 1), β is full width at half maximum-FWHM (β = 1.606). The calculation result is 12.3 nm.Figure 1. XRD pattern of the magnetite nanoparticles In the figure 2 shown FT-IR spectra of uncoated and coated Fe 3O 4 nanoparticles. It can be seen that, compared with the uncoated sample, the coated Fe 3O 4 nanoparticles posses adsorption bands in 1068 cm -1 due to the stretching vibration of Si-O bond, band in 792 cm -1 due to the bending vibration of –NH 2 group. All these reveal the existence of APTS. In addition, in figure 5 (a) and (b) the absorption bands near 3400 and 1630 cm -1 refer to the vibration of remainder H2O in the samples, bands near 2920 and 2850 cm -1 due to stretching vibration of C-H bond, bands near 570 cm -1 due to stretching vibration of Fe-O, bands near 1400 cm -1 due to stretching vibrations of N-H.immobilized on magnetic carriers.Figure 2. FT-IR spectra of the uncoated (a) and coated (b) magnetite nanoparticles3.2 Immobilization of LipaseThe magnetite as support was selected because it has some advantages (Huang, Liao et al., 2003): (i) higher specific surface area obtained for the binding of a larger amount of enzymes, (ii) lower mass transfer resistance and less fouling, and (iii) the selective separation of immobilized enzymes from a reaction mixture by the application of a magnetic field (Halling and Dunnill, 1980). Magnetite (Fe3O4) is one of the famous magnetic materials in common use. As a result of strong magnetic property and low toxicity, its applications in biotechnology and medicine have gained significant attention (Curtis and Wilkinson, 2001).In addition and with the interest to increase this resistance, the addition of glutaraldehyde as covalent link allows improving the properties already mentioned and it increases the degree of acetylation of the support which leads to a bigger fixation of lipase and also to a greater degree of hydrofobicity avoiding the possible catalyst and poisoning (Shao-Hua and Wen-Teng, 2004).Finally to carry out the fixation of the enzyme in the support has been used the impregnating technique, submerging the support with glutaraldehyde in a solution of known concentration and determining the percentage of immobilization of theenzyme by means of espectofotometric tests; the obtained results indicate that the percentage of immobilization of the enzyme is of 40%. These results are corroborated with the Kjeldhal method which determined the total nitrogen (5.84%) that multiply for a constant (k = 6.5) gives the percentage of immobilization (38%). This result is similar to calculated for espectofotometric test.4.ConclusionsLipase was directly bound to Fe3O4 magnetic nanoparticles via glutaraldehyde activation. The analyses of XRD indicated the resultant magnetic nanoparticles were pure Fe3O4 with a mean diameter of 12.3 nm. FTIR spectra were utilized to prove the formation of Fe-O-Si chemical bonds. The percentage of lipase immobilization was 40% and this data were calculated for Kjeldhal method and espectofotometric test. The enzyme-linked, it has been proved that these APTS-coated magnetite nanoparticles could significantly improve the protein immobilization.The process of extractive reaction is begins developed for biodiesel production from palm or castor oil with ethanol catalyzed for immobilized lipase on magnetic particle. This process can be modeling and simulated with the principles of processes intensification and the methodology that has been developed for the Group Investigation: Chemical, Catalytic and Biotechnology Processes of the National University of Colombia at Manizales. The obtained results in the modeling, simulation and experimentation will make part of future publications.Finally, this work will be helpful for the practical application of lipase and of other enzymes.AcknowledgmentsThis work was supported by the Direction of Investigations of the National University of Colombia at Manizales (DIMA).ReferencesClark, S. J., Wagner, L., et al., (1984) J. Am. Oil Chem. Soc., 61, 1632-1638. Curtis, A. and Wilkinson, C., (2001) Trends Biotechnol., 19, 97-101.Chen, J.-P. and Lin, W.-S., (2003) Enzyme and Microbial Technology, 32, 801-811. Foglia, T. A., Nelson, L. A., et al., (1997) J. Am. Oil Chem. Soc., 73, 1191–1195. Halling, P. J. and Dunnill, P., (1980) Enzyme Microb. Technol., 2, 2-10.Hsu, A.-F., Jones, K. C., et al., (2002) Biotechnol. Appl. Biochem., 36, 181-186. Hsu, A.-F., Jones, K. C., et al., (2001) J. Am. Oil Chem. Soc., 78, 585–588.Huang, S.-H., Liao, M.-H., et al., (2003) Biotechnol. Prog., 19, 1095-1100.immobilized on magnetic carriers.Kuncová , G. and Sivel, M., (1997) J. Sol-Gel Sci. Technol., 8, 667-671.Ma, M., Zhang, Y., et al., (2003) Colloids and Surfaces A: Physicochem. Eng. Aspects, 212, 219-226.Mihama, T., Yoshimoto, T., et al., (1988) J. Biotechnol., 7, 141-146.Moore, D. and Reynolds, R., X-Ray Diffraction and the Identification and Analysis of Clay Minerals, Oxford University Press, New York (1997).Nelson, L. A., Foglia, T. A., et al., (1996) J. Am. Oil Chem. Soc., 73, 1191–1195. Reetz, M., Zonta, A., et al., (1998) J. Mol. Catal. A 134, 251-258.Samant, K., (1998) AIChE J. , 44, 1363-1381.Selmi, B. and Thomas, D., (1998) J. Am. Oil Chem. Soc., 75, 691-695.Shao-Hua, C. and Wen-Teng, W., (2004) Biomaterials, 25, 197-204.Shimada, Y., Watanabe, Y., et al., (1999) J. Am. Oil Chem. Soc., 76, 517-521. Tamaura, Y., Takahashi, K., et al., (1986) Biotechnol. Lett., 8, 877-880.Tartaj, P., Morales, M. P., et al., (2005) Journal of Magnetism and Magnetic Materials, 290-291, 28-34.Zeng, L., Luo, K., et al., (2006) Journal of Molecular Catalysis B: Enzymatic, 38, 24-30.。

尊严疗法的效果评价及其在我国的应用与研究

尊严疗法的效果评价及其在我国的应用与研究

尊严疗法的效果评价及其在我国的应用与研究赵圣敏;闫翔宇;赵曼羽;方一安;陈慧平【摘要】“尊严疗法”是一种新兴的心理治疗方法,国外大量研究证实其在提升生命末期患者尊严感、减轻心理痛苦等方面具有显著效果.但尊严疗法在中国的临床应用和研究还处于起步阶段,在其具体的实施过程中仍存在一些问题,且由于东西方文化差异和社会经济发展水平不同,需从医护人员、生命末期患者及患者家属角度出发,进一步探索适合中国国情的生命朱期患者心理特征的实施方案,以改善他们的心理状况,提高他们的生命意义感和存在感,从而提升其生命质量.【期刊名称】《医学与哲学》【年(卷),期】2019(040)002【总页数】4页(P49-51,70)【关键词】尊严疗法;生命末期;心理治疗;生命质量【作者】赵圣敏;闫翔宇;赵曼羽;方一安;陈慧平【作者单位】四川大学华西公共卫生学院四川成都 610041;四川大学华西公共卫生学院四川成都 610041;四川大学华西公共卫生学院四川成都 610041;四川大学华西公共卫生学院四川成都 610041;四川大学华西第四医院四川成都 610041【正文语种】中文【中图分类】R-05相关研究显示,国外有3/4的临终患者遭受着身心折磨[1]。

孤独无助感和失落感是临终患者面临的两个主要的心理问题[2]。

进入临终期的患者,身体机能日益衰竭,基本技能逐渐丧失,很多事情都必须依赖他人完成,他们在遭受着躯体疼痛的同时,尊严感也会因此降低,甚至丧失生存欲望,出现自杀念头。

患者因常常想到死亡的不可抗拒而出现以绝望、恐惧、忧郁为主的心理特征[3]。

越来越多的学者及医护人员关注到了这个问题,临床上也开始不单以治疗身体疾病、延长生存时间为唯一目标,患者在治疗过程中心理、精神等方面的问题也不断地受到重视,维护临终患者的个人尊严逐渐成为临床治疗过程必不可少的任务。

在此背景下,如何提高临终患者的尊严,提升患者生命质量和生存欲望,让生命尊严落幕,成为一个医学界乃至全社会亟待解决的问题,也是医护领域一个重要的研究方向。

信息检索综合考查作业

信息检索综合考查作业

层间氧化带砂岩型铀矿根据国际原子能机构(IAEA)报道,至2001年全世界现己探明的铀资源量中砂岩型铀矿位于各铀矿类型的第二位,占世界探明资源量的1/4强,仅次于在澳、加两国占统治地位的不整合面型铀矿(王正邦,2002;陈祖伊,2003),而其矿床数量占世界探明矿床数量的42.9%,分布范围最广泛(周维勋等,2000),占世界超大型铀矿床(储量大于5万吨)总数的1/3。

砂岩型铀矿根据成因和矿化特征主要分成晚期成岩表生后生渗入叠加型砂岩型铀矿、表生后生渗入型砂岩型铀矿、表生后生渗出渗入型砂岩型铀矿、后生热水叠造型砂岩型铀矿(王正邦,2002)。

层间氧化带砂岩型铀矿属于飙升后生渗入型砂岩型铀矿,具有埋藏浅、适宜地浸开采、分布较普遍和矿床规模大等最具开发价值。

目前,砂岩型铀矿床,特别是可地浸层间氧化带砂岩型铀矿床在世界铀资源中的地位举足轻重,己成为世界铀矿找矿和开发的主攻类型之一。

一、国内外研究现状1.1国外研究现状二十世纪五十年代以来,前苏联、美国等层间氧化带砂岩型铀矿勘查取得了巨大的成功,有关研究也各具特色,形成了―层间氧化带‖和―卷状‖砂岩型铀矿成矿理论两大流派。

成因类型上分为―整合型‖和―卷型‖两大类,形成著名的―卷型‖砂岩铀成矿理论。

在产铀盆地分析、成矿地质、地球化学环境、成矿物质来源、成矿作用机制、找矿判据等研究领域都取得了重要的研究成果,发表了大量的专著和论文。

代表性成果有:H.H.Adler和B.J.Sharp(1967)对卷型铀矿床的地质构造背景、含铀建造沉积环境和成因作了全面总结;R.L.Rackley(1976)对砂岩型铀矿的成因作了深入探讨;E.N.Harshman和S.S.Adams(1981)研究了美国陆相砂岩中卷型铀矿床的地质一地球化学模式和识别判据。

W.RKeefer(1970)对温得河盆地(Wind River Basin)构造背景的研究;J.W. King和S.R.Austin(1965, 1966)对怀俄明盆地气山(Gas Hills)卷型铀矿床的总结;R.E.Melin (1964,1969)对谢利盆地(Shirley Basin)铀矿床的成因研究;D.H.Earhle等(1961)对得克萨斯州东南部海岸平原铀矿床的形成及与油田断裂带中含硫化氢碳氢化合物之间关系进行了探讨;L.Jenson(1958)对砂岩型铀矿床成因与硫同位素的研究;K.R.Ludwig(1978,1979)关于铀矿化年龄的研究;M.F.Kashirtseva(1964)对砂岩型铀矿床中矿物与地球化学分带的研究;W.F.Galloway(1979,1984)对南得克萨斯砂岩型铀矿沉积环境和水文地质条件的研究;P.E.Hart, R.O.Duda和M.T. Einaudi(1978 )及J.Gaschaig (1980)利用计算机对铀矿勘探专家系统的研究;E. N.Harshman与S.S.Adams(1981)对砂岩型铀矿识别判据体系的研究和总结。

IEEE-certification of reference

IEEE-certification of reference

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Stegun, Eds., Handbook of Mathematical Functions (Applied Mathematics Series 55).Washington, DC: NBS, 1964, pp. 32-33.[5] E. F. Moore, “Gedanken-experiments on sequential machines,” in Automata Studies (Ann. of MathematicalStudies, no. 1), C. E. Shannon and J. McCarthy, Eds. Princeton, NJ: Princeton Univ. Press, 1965, pp. 129-153.[6] Westinghouse Electric Corporation (Staff of Technology and Science, Aerospace Div.), Integrated ElectronicSystems . Englewood Cliffs, NJ: Prentice-Hall, 1970.[7] M. Gorkii, “Optimal design,” Dokl. Akad. Nauk SSSR , vol. 12, pp. 111-122, 1961 (Transl.: in L. Pontryagin, Ed.,The Mathematical Theory of Optimal Processes. New York: Interscience, 1962, ch. 2, sec. 3, pp. 127-135).[8] G. O. Young, “Synthetic structure of industrial plastics,” in Plastics, vol. 3, Polymers of Hexadromicon , J. Peters, Ed., 2nd ed. New York: McGraw-Hill, 1964, pp. 15-64. [1] Name of Manual/Handbook , x ed., Abbrev. Name of Co., City of Co., Abbrev. State, year, pp. xx-xx. 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Hall技术治疗乳磨牙龋齿的临床研究

Hall技术治疗乳磨牙龋齿的临床研究

Hall技术治疗乳磨牙龋齿的临床研究摘要:目的:评价乳磨牙龋病治疗中使用Hall技术的临床效果。

方法:选取在2022年1月~2023年2月于我院需行乳磨牙龋齿修复治疗的患儿83例,共133颗患牙,随机数字表法分为传统预成冠治疗组66颗,Hall技术治疗组67颗,随访6个月、12个月、18个月,观察远期临床疗效。

结果:6、12及18个月后Hall技术治疗组成功率为98.51%、98.51%和94.03%,传统预成冠治疗组成功率均为98.48%,两组比较差异无统计学意(P>0.05);Hall技术治疗组时间短于传统预成冠治疗组,家属满意度评分高于传统预成冠治疗组,差异均有统计学意(P<0.05)结论:Hall技术既具有无创特点又具有冠修复优势,缩短治疗时间,提高患者满意度,可以临床运用于乳磨牙龋齿治疗。

关键词:乳磨牙,Hall技术,预成冠乳磨牙龋损是儿童口腔牙病中最常见疾病之一。

传统的树脂充填技术已经逐渐被金属预成冠修复技术所取代,金属预成冠修复技术是局麻下去除腐质后充填后预成冠修复或者直接传统金属预成冠修复[1]。

2006年,苏格兰牙医NornaHall首次提到了Hall技术,Hall技术是基于金属预成冠技术提出的无须局麻、去腐及任何牙体预备,直接将冠用玻璃离子粘固到龋损的乳磨牙上,从而达到控制龋损的发展、保留乳磨牙的治疗方法。

本研究通过临床随机对照,比较Hall技术和普通金属预成冠技术治疗乳磨牙龋齿的效果。

现报告如下。

1一般资料1.1一般资料和方法选取2022年1月~2023年2月于我院需行乳磨牙龋齿修复治疗的患儿83例,男45人,女38人,共133颗患牙,上乳磨牙65颗,下乳磨牙68颗,随机数字表法分为传统预成冠治疗组66颗,Hall技术治疗组67颗,年龄范围4-9岁,平均年龄(7.10±2.89)岁。

本研究获得伦理委员会批准,且患儿及其家属均签署知情同意书。

电话回访并预约患儿及家属来我院进行复查。

风痛宁片中麻黄碱含量测定及其影响因素分析开题报告

风痛宁片中麻黄碱含量测定及其影响因素分析开题报告
样品 配制 鉴别 麻黄 提取 含量 测定 分析 影响 因素 分析 数据 分析
2.1 对照品溶液制备 精密称取 105℃干燥至恒重的盐酸麻黄碱适量, 置量瓶中, 加水溶解稀释 至刻度,摇匀,即得。 2.2 供试品溶液制备 取风痛宁片适量,精密称定,研细,置蒸馏瓶中,加 20﹪氢氧化钠液适 量,水蒸汽蒸馏。收集馏出液,加水稀释至刻度,摇匀,即可。 2.3 波长选择 取对照品 0.8mL 溶液, 加水 4.2mL 及供试品溶液 5mL, 分别加入碳酸钠试 液 1mL,过碘酸钠试液 2mL,摇匀。放置 10min,分别精密加入正己烷 20mL。 振荡 30s,静置 10min 使分层。分取正己烷层,即得对照品和供试品测定液。 在 200-400nm 波长范围内进行光谱扫描。 2.4 含量测定 精密量取对照品溶液和供试品溶液适量,按 2.3 项下操作,在 242nm 波 长处测得麻黄碱的含量。按外标一点法计算风痛宁片中麻黄碱的含量。 2.5 稳定性试验
1
参考文献: [1] 柯长江,马辉明.紫外分光法测定半夏露口含片中麻黄碱的测定[J].药物分析 杂志,1999,19(1):47-54. [2] 姚新标,张文文.盐酸麻黄碱稳定性试验[J].新疆中医药,2005,23(2):10-11. [3] 莫明兴,费寿耆.紫外分光光度法测定麻黄素洗必泰滴鼻液的含量[J].海峡药 学,2002,14(1):42-52. [4] 李俐,陈坚.麻黄及其有效成分研究进展[J].新疆医科大学学报 2003,26(6): 6 06-608. [5] 李计萍.中药新药稳定性研究的现状及思考[J].江西中医学院学报,2004,6 (5):25-28. [6] 李继珩,王鲁燕,李平.铜离子络合法测定盐酸麻黄素含量[J].中国药科大学 学报,2000,3(4):27-31. [7] 陈叶琴.紫外分光光度法测定咳嗽合剂 I 中盐酸麻黄碱的含量[J].江苏药学与 临床研究,2004,12(2):18-19. [8] 陈桂新,李凤祥,曹顺林, 陈文成.息喘灵片中盐酸麻黄碱的含量测定[J].滨州 医学院学报,1999,22(2):97-101. [9] Jesper Hallas, Lars Bjerrum, Henrik Stvring, and e of a Scribed Ephedrine/Caffeine Combination and the Risk of Serious ardiovascular Events: A Registry-based Case-Crossover Study. 2008, 168(8): 123-154. [10] Guoyi Ma, Supriya A.Bavadekar, Yolande M.Davis, Shilpa lchandani, Phar macological Effects of Ephedrine Alkaloids on Human 1-and 2-Adrenergic Rece ptor Subtypes. 2007, 322(1): 214-221. [11] 廖昌军,杨拯.紫外分光光度法测定盐酸麻黄碱滴鼻液的含量[J].成都医学 院院报,2006,1(2):126-128. [12] 宋金英.哮灵片中盐酸麻黄碱的含量测定[J].长春中医药大学学报报, 2007, 12(6):66-67. [13] J.K.Dunnick, G.Kissling, D.K.Gerken, et al. Cardiotoxicity of Ma Huang/Caffein e or Ephedrine/Caffeine in a Rodent Model System. 2007, 35(5): 206-208.

四级硬度 缔造性福

四级硬度  缔造性福

四级硬度 缔造性福 1-4
1. Mulhall J, et al. J Sex Med. 2007;4(2):448-464. 2. King R, et al. Int J Impot Res. 2011;23(4):135-141. 3. Jünemann KP, et al. J Sex Med. 2006;3(suppl 3): 252. (full version) 4. Mulhall JP, et al. Urology. 2006;68(Suppl 3A):17-25.
本研究入选979名ED患者,进行长达4年的开放性研究,给予患者灵活剂量西地那非(25mg、50mg或 100mg)按需服用,并于每年末评估对勃起的治疗效果感到满意的ED患者比例。
McMurray JG, et al. Ther Clin Risk Manag. 2007;3(6):975-981.
总结
4
*
4.2次
每月达到第4级勃起硬度 的次数
3
2
1
0.7次
0 安慰剂(n=205)
西地那非50mg(n=105)
这是一项为期24周的随机、双盲、安慰剂对照研究,532名ED患者接受西地那非(25mg、50mg 或100mg)或安慰剂治疗,观察勃起硬度的改善。
Goldstein I, et al. N Engl J Med. 1998;338(20):1397-1404.
3810100605121620所有治疗相关的不良事件头痛消化不良鼻炎潮红収生率05治疗相关不良事件本研究入选979名ed患者进行长达4年的开放性研究给予患者灵活剂量西地那非25mg50mg或100mg按需服用幵亍每年末评估对勃起的治疗效果感到满意的ed患者比例

铁离子对迟缓爱德华氏菌生物膜形成和基质组成的影响-湖南

铁离子对迟缓爱德华氏菌生物膜形成和基质组成的影响-湖南

铁离子对迟缓爱德华氏菌生物膜形成和基质组成的影响-湖南湖南省大学生研究性学习和创新性实验计划项目申报表项目名称: 铁离子对迟缓爱德华氏菌生物膜形成和基质组成的影响学校名称湖南农业大学学生姓名学号专业性别入学年份钟为铭201440566114 水族科学男2014级钟浪201440566118 水族科学男2014级叶涛201440566113 水族科学男2014级王雅琳201440566111 水族科学男2014级陈康勇201440566135 水族科学男2014级指导教师高志鹏职称讲师项目所属一级学科水产学学生曾经参与科研的情况参与学生均无参与科研的经历。

指导教师承担科研课题情况1、国家自然基金面上项目,鲶爱德华氏菌Ⅲ型与Ⅵ型分泌系统致病的分子机制研究,2010-2012,参与。

2、国家自然基金面上项目,鲶爱德华氏菌多拷贝质粒pEI1和pEI2编码的毒力蛋白的分子致病机理研究,2012-2015,参与。

-2-项目研究和实验的目的、内容和要解决的主要问题一、目的和意义迟缓爱德华氏菌作为重要的鱼类病原菌,其致病性与生物膜形成密切相关,而Fe3+又与细菌生物膜的形成、结构和基质组成密切相关。

因此,本研究拟通过结晶紫染色、CLSM、SEM和FilmTracer™ LIVE/DEAD® kit染色等方法,研究Fe3+在迟缓爱德华氏菌生物膜形成、结构和生物膜内细菌生存能力等方面的作用;通过利用聚丙烯酰胺凝胶电泳(SDS-PAGE)技术和SYPRO® Ruby 染色、FITC-ConA染色和DDAO染色等方法分别研究Fe3+对迟缓爱德华氏菌生物膜基质中蛋白质、多糖和胞外DNA的分布和形成的影响。

最终阐明Fe3+在迟缓爱德华氏菌生物膜形成中的作用机理,迟缓爱德华氏菌与耶尔森氏菌和沙门氏菌等人类病原菌同属于肠杆菌科,研究Fe3+在迟缓爱德华氏菌生物膜形成中的作用机理,可为人类主要肠道病原菌的相关研究提供重要的理论依据。

心理学学术论文:结构方程建模中的题目打包策略

心理学学术论文:结构方程建模中的题目打包策略

心理学学术论文:结构方程建模中的题目打包策略吴艳/温忠麟 【内容提要】结构方程建模中题目打包法的优缺点包括:指标数据质量变好、模型拟合程度提高;估计偏差不大,可校正;估计稳定,但降低了敏感性与可证伪性。

打包法的前提条件是单维、同质,适合结构模型分析,不适合测量模型分析。

对于单维测验,给出了一个打包流程。

对于通常的多个子量表(多维结构)测验,推荐在子量表内打包,每个子量表打包成1个指标或者3个指标,用于结构方程建模。

【关键词】潜变量/结构方程/题目打包/题目小组 1、前言结构方程建模,对样本容量有一定的要求。

有建议说样本容量应当是题目(指标)数量的10倍以上,或者是自由参数的5倍以上(侯杰泰,温忠麟,成子娟,2004 )。

可见,题目越多,所需样本容量越大。

题目数量很多时,要估计的参数也多,若样本容量少,直接用原始题目建模容易产生较大的参数估计偏倚。

Bandalo s(2002)的模拟研究显示,在单维、样本容量为100,严重非正态的情况下,直接使用原始题目建模可产生高达29.5%的参数估计偏倚。

题目打包法(item parceling,也译为题目组合法或题目小组法)是解决此类问题的一种有效方法( Landis, Beal, & Tesluk, 2000)。

题目打包法是将同一量表的两个或以上题目打包成一个新指标,用合成分数(总分或均值)作为新指标的分数进行分析(Kishton& Widaman, 1994; Yang, Nay, & Hoyle, 2010)。

例如,一个量表原来有9个题目,可将每3个题目作为一个题目小组计算合成分数(小组内题目数也可以不相等),形成3个新指标,打包法直接用3个新指标进行分析(Little,Cunningham, Shahar, & Widaman, 2002; Rogers &。

2.2打包法偏差不大、可校正应用研究者关心的一个重要问题是,使用打包法与直接使用原始题目相比,参数估计是否会产生偏差呢?Yuan,Bentler和Kano(1997)的研究显示,当题目在总体中单维、同质时,使用打包法与直接使用原始题目相比,结构参数估计不变(也见Sass& Smith, 2006)。

APA格式参考文献示例

APA格式参考文献示例

APA格式参考文献示例期刊文章1.一位作者写的文章Hu, L. X. [胡莲香]. (2014). 走向大数据知识服务: 大数据时代图书馆服务模式创新. 农业图书情报学刊(2): 173-177.Olsher, D. (2014). Semantically-based priors and nuanced knowledge core for Big Data, Social AI, and language understanding. Neural Networks, 58, 131-147.2.两位作者写的文章Li, J. Z., & Liu, X. M. [李建中, 刘显敏]. (2013). 大数据的一个重要方面: 数据可用性. 计算机研究与发展(6): 1147-1162.Mendel, J. M., & Korjani, M. M. (2014). On establishing nonlinear combinations of variables from small to big data for use in later processing. Information Sciences, 280, 98-110.3. 三位及以上的作者写的文章Weichselbraun, A. et al. (2014). Enriching semantic knowledge bases for opinion mining in big data applications. Knowledge-Based Systems, 69, 78-85.Zhang, P. et al. [张鹏等]. (2013). 云计算环境下适于工作流的数据布局方法. 计算机研究与发展(3): 636-647.专著1.一位作者写的书籍Rossi, P. H. (1989). Down and out in America: The origins of homelessness. Chicago: University of Chicago Press.Wang, B. B. [王彬彬]. (2002).文坛三户:金庸·王朔·余秋雨——当代三大文学论争辨析. 郑州: 大象出版社.2.两位作者写的书籍Plant, R., & Hoover, K. (2014). Conservative capitalism in Britain and the United States: A critical appraisal. London: Routledge.Yin, D., & Shang, H. [隐地, 尚海]. (2001).到绿光咖啡屋听巴赫读余秋雨. 上海: 上海世界图书出版公司.3. 三位作者写的书籍Chen, W. Z. et al. [陈维政等]. (2006).人力资源管理. 大连: 大连理工大学出版社. Hall, S. et al. (1991). Culture, media, language: Working papers in cultural studies, 1972-79 (Cultural studies Birmingham ). London: Routledge.4. 新版书Kail, R. (1990). Memory development in children (3rd ed.). New York: Freeman.编著1. 一位主编编撰的书籍Loshin, D. (Ed.). (2013a). Big data analytics. Boston: Morgan Kaufmann.Zhong, L. F. [钟兰凤] (编). (2014). 英文科技学术话语研究. 镇江: 江苏大学出版社.2. 两位主编编撰的书籍Hyland, K., & Diani, G. (Eds.). (2009). Academic evaluation: Review genres in university settings. London: Palgrave Macmillan.Zhang, D. L., & Zhang, G. [张德禄, 张国] (编). (2011). 英语文体学教程. 北京: 高等教育出版社.3. 三位及以上主编编撰的书籍Zhang, K. D. et al. [张克定等] (编). (2007). 系统评价功能. 北京: 高等教育出版社.Campbell, C. M. et al. (Eds.). (2003). Groups St Andrews 2001 in Oxford: Volume 2.New York: Cambridge University Press.4.书中的文章De la Rosa Algarín, A., & Demurjian, S. A. (2014). An approach to facilitate security assurance for information sharing and exchange in big-data applications. In B.Akhgar & H. R. Arabnia (Eds.), Emerging trends in ICT security(pp. 65-83).Boston: Morgan Kaufmann.He, J. M., & Yu, J. P. [何建敏, 于建平]. (2007). 学术论文引言部分的经验功能分析.张克定等. (编). 系统功能评价(pp. 93-101). 北京: 高等教育出版社.翻译的书籍Bakhtin, M. M. (1981). The dialogic imagination: Four essays(C. Emerson & M.Holquist, Trans.). Austin: University of Texas Press.Le, D. L. [勒代雷]. (2001).释意学派口笔译理论(刘和平译). 北京: 中国对外翻译出版公司.Kontra, M. et al. (2014).语言: 权利和资源(李君, 满文静译). 北京: 外语教学与研究出版社.Wang, R. D., & Yu, Q. Y. [王仁定, 余秋雨]. (2001).吴越之间——余秋雨眼里的中国文化(彩图本)(梁实秋, 董乐天译). 上海: 上海文化出版社.硕博士论文Huan, C. P. (2015). Journalistic stance in Chinese and Australian hard news.Unpublished doctorial dissertation, Macquarie University, Sydney.Wang, X. Z. [王璇子]. (2014). 功能对等视角下的英语长句翻译.南京大学硕士学位论文.注:1.APA格式参考文献中的文章标题、书籍名称,冒号后第一个单词,括号里第一个单词和专有名词的首字母大写,其余单词首字母均小写。

有创足背动脉压与无创肱动脉压测量的对比研究

有创足背动脉压与无创肱动脉压测量的对比研究

有创足背动脉压与无创肱动脉压测量的对比研究上官云芳段思源姚颖【摘要】目的探讨当有创收缩压(ABP)处于不同范围时无创收缩压(NIBP)与ABP 的关系。

方法选取我院2012年6月~2013年5月期间入院即将进行消化道肿瘤手术的患者50例,选用右侧肱动脉测量NIBP,选用足背动脉测量ABP。

所有患者均于静吸复合麻醉下进行手术,记录所有患者足背动脉穿刺诱导后1分钟(T1)、气管插管瞬间(T2)、插管后2分钟(T3)、手术切皮时(T4)各个时间点上的NIBP和相应的ABP,选取111mmHg为临界值,对ABP不同范围内不同时点ABP与NIBP之间的差异进行比较。

结果(1)当ABP<111mmHg 时,仅在插管后2分钟时ABP值大于NIBP,且差异有统计学意义(P<0.05);而在诱导后1分钟(T1)、气管插管瞬间(T2)和手术切皮时(T4)ABP与NIBP 之间的差异没有统计学差异(P>0.05)。

(2)当ABP≥111mmHg时,各个时间点上的ABP值均大于NIBP值,且差异均有统计学差异(P<0.0001);结论当消化道肿瘤手术患者足背ABP高于111 mmHg时,ABP高于NIBP,而当ABP低于111 mmHg时,ABP并不低于NIBP。

【关键词】消化道肿瘤手术;有创动脉血压;无创动脉血压;足背动脉Comparison of arterial blood pressure and non-invasive blood pressureShangguan yun-fang,Duan si-yuan, Yao yin[Abstract] Objective To investigate the relationship between NIBP and ABP when ABP in different range. Methods A self-control study was conducted among 50 cases of patients prepared to accept gastrointestinal tumor operation from June 2012 to May 2013. The blood pressure of brachial artery was measured as NIBP and the blood pressure of dorsalis pedis artery were measured as ABP simultaneously. All patients received under inhalation anesthesia during surgery, NIBP and ABP were recorded after one minute induction (T1), tracheal intubation moment (T2), two minutes after intubation(T3) and the skin incision moment(T4). Systolic pressure of 111mmHg was selected as cut-off point, differences between ABP and NIBP at various time points were compared. Results When ABP<111mmHg, ABP is higher than NIBP (P<0.05)at T2 time point, while no statistical difference between ABP and NIBP appears at other time points. When ABP≥111mmHg, ABP is higher than NIBP at each time points (P<0.0001). Conclusion ABP is higher than NIBP when ABP ≥110mmHg, while ABI is not lower than NIBP when ABP < 110mmHg among gastrointestinal tumor operation patients.Key words Gastrointestinal tumor operation, Arterial Blood Pressure, Non-Invasive Blood Pressure, Dorsalis Pedis Artery血压是反映人体循环系统机能的重要指标,也是临床上用于监测患者生命体征的重要参数之一。

纳米银颗粒在不同溶液中的聚集动力学

纳米银颗粒在不同溶液中的聚集动力学

纳米银颗粒在不同溶液中的聚集动力学张强【摘要】采用Tollens还原反应用柠檬酸钠做分散剂制备了纳米银粒子(AgNP).利用透视电子显微镜(TEM)及动态光散射法(DLS)对纳米银的形貌、粒径分布及在不同盐溶液中的平均粒径进行了表征;同时采用TR-DLS技术对纳米银在不同自然水体中的聚集动力学进行了研究.结果表明:以柠檬酸钠为分散剂的纳米银主要呈球形,平均粒径为30 nm;实验表明,纳米银在海水中的聚集速率最快,在地下水中次之,在地表淡水中聚集速率最小.本研究对纳米银在环境水体中的环境危害评估有重要的借鉴意义.【期刊名称】《广州化工》【年(卷),期】2013(041)017【总页数】3页(P78-79,82)【关键词】纳米银;Tollens法;聚集动力学;聚集速率【作者】张强【作者单位】济南化工厂,山东济南250001【正文语种】中文【中图分类】O614.122统计数据表明,近些年,纳米银粒子(AgNP)被广泛应用于抗菌材料领域[1-9]。

纳米银材料已经占纳米材料市场总份额的23%以上[8]。

目前研究表明,制备AgNP的方法有化学还原法、生物法、及物理法等。

其中,化学还原法被广泛应用于科学研究及工业化生产等领域。

在化学还原法中,Tollens法因其简单的合成步骤及其环境友好性被广泛使用。

常用的纳米银稳定剂包含十二烷基硫酸钠(SDS)、吐温80(Tween-80),聚乙烯吡咯烷酮等作为分散剂常为Tollens法采用[10-11]。

本研究采用柠檬酸盐作为分散剂,柠檬酸盐的还原性有助于Tollens反应的进行,且其具有便宜易得及良好的生物降解性等优点。

作为有效的抗菌纳米材料,释放到环境中的纳米银的毒性与其物理化学性质如表面电荷与颗粒大小直接相关[10-15],因此,研究纳米银颗粒的聚集动力学对其颗粒粒径的预测有重要意义。

虽然已有大量文献研究了纳米银在不同电解质中的聚集速率,但是,对于纳米银在不同自然水体中的聚集动力学的研究报道却一直很少。

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Disruptive technologies, stakeholders and the innovation value-added chain:a framework for evaluating radical technology developmentJeremy K.Hall1and Michael J.C.Martin21University of Calgary,Calgary,Canada.jeremy.hall@haskayne.ucalgary.ca2University of Exeter,Exeter,UK.m.j.c.martin@Contemporary frameworks for evaluating technological innovations contend that innovative success is dependent upon the ability offirms to acquire and assimilate new knowledge without disrupting value chain members such as suppliers,customers and complementary innovators.These frameworks,however,provide little advice on how to deal with radical,controversial innovations that may also introduce new undesirable environmental,health,and social side affects.In addition to technological,commercial and organisational uncertainties,the developers of such technology typically must resolve social uncertainties,a particularly difficult activity because of the added complexities and often conflicting and/or difficult-to-reconcile concerns from secondary stakeholders.Attempts must be made to address the potential unintended and unforeseen consequences of the technology,as well as its potential benefits,if it is to be successfully ing Monsanto’s development of agricultural biotechnology as an illustration,we suggest an evaluation framework that incorporates stakeholder theory,innovation management concepts and Popper’s evolutionary learning methodology of science and its extension to social issues.1.Introduction:radical technologydevelopmentT echnological innovation has long been recog-nised as a difficult and expensive activity,yet often a key to survival and a main source of sustained growth forfirms(Schumpeter,1934, 1942;Penrose,1959).It has the potential to generate unique,difficult to imitate organisa-tional capabilities and competencies leading to competitive advantage(Teece et al.,1997;Eisen-hardt and Martin,2000;Zollo and Winter,2002),but can also be competency destroying(Tushman and Anderson,1986)or disruptive(Christensen, 1997).Current management of technology ap-proaches emphasise the need to recognise the disruptive potential of an innovation on indus-tries(Utterback,1994;Christensen,1997),firms (Tushman and Anderson,1986;Henderson and Clark,1990;Leonard,1995)and innovating sup-ply chains/networks(Afuah and Bahram,1995; Afuah,1998).These approaches may be sufficient for an innovation that solely disrupts its existing industry and market base,as has happened withR&D Management35,3,2005.r Blackwell Publishing Ltd,2005.Published by Blackwell Publishing Ltd,273 9600Garsington Road,Oxford OX42DQ,UK and350Main Street,Malden,MA02148,USA.for example digital photography(Munir,2002). However,they pay scant attention to innovations that may have potentially controversial disruptive impacts on societal third parties,those that Free-man(1984)calls secondary stakeholders.For the purpose of this paper,the term radical technology development is applied to radical technological innovations(often initiating new industries)that are controversial and potentially disruptive to secondary stakeholders.A recent example is the development of genetic technology in agriculture.Given the potentially widespread implications of such developments,consideration must be made for the innovatingfirm’s compe-tencies as well as its impact on secondary stake-holders that may affect,or be affected by the technology.We thus suggest the inclusion of a broader stakeholder analysis,as originally sug-gested by Freeman(1984)and specifically devel-oped within the context of innovation by Hall and Vredenburg(2003)and R&D management by Elias et al.(2002).However,we note that there are added challenges because of the presence of many interacting stakeholders(i.e.greater com-plexity),which may not be easily identified and/or may have contradictory demands,goals or inter-ests(i.e.greater stakeholder ambiguity).To manage these additional challenges,we suggest a framework for considering the added complexity and uncertainty of innovation when stakeholder perceptions and information are especially important.Such situations include ap-plications of emerging radical technologies such as genetic technology and nanotechnology,where the public understanding of science may be a factor and where there is concern about possible environmental,health and social side effects. Building on Afuah’s(1998)innovation value-added chain and Freeman and Soete’s(1997) innovation uncertainties,we distinguish between largely well-recognised technological,commer-cial,and organisational uncertainties as well as the more elusive uncertainties from social side effects.Under circumstances of relatively low complexity and stakeholder ambiguity(e.g.pri-mary stakeholders such as suppliers,customers and complementary innovators),a trial-and-er-ror-elimination learning approach(Popper,1959) is appropriate.In the wider societal secondary stakeholder context of not immediately recog-nised higher complexity and ambiguity,a piece-meal social engineering approach(Popper,1945)is appropriate.We illustrate our approach with Monsanto’s attempts to develop genetic technol-ogy in global agriculture markets.12.Managing innovative uncertainty2Like all innovation,radical technology develop-ment can be viewed as a knowledge quest and creation process within thefirm’s social networks (Nonaka et al.,2000)requiring the reduction of uncertainty(Katz and Shapiro,1985;Arthur, 1988;Freeman and Soete,1997).Uncertainty is particularly high for radical technology develop-ment because it may involve new science,tech-nology,markets,industrial structures,regulatory frameworks,etc.(Ansoff,1957;Utterback,1994). There are thus greater degrees and types of uncertainties for radical technology develop-ments,which for the purpose of this discussion, can be categorised as being concerned with tech-nological feasibility,commercial viability,orga-nisational capability and social acceptability. These levels roughly correspond to Freeman’s (1982)categories of innovation uncertainty(tech-nical,market and political/economic uncertainty) and Rice et al’s(2001)radical technology-to-organisation links(technology,market,and cor-porate strategy-related issues)and are discussed in more detail below.The new knowledge quest typically concen-trates on technological,commercial and organi-sational variables from the perspective of the individualfirm.For example,seminal work by Henderson and Clark(1990)recognised the diffi-culties if introducing new technological knowl-edge(either architectural,component or both)on incumbentfirms,whereas Abernathy and Clark (1985)and Martin(1994)emphasised the impor-tance of new market knowledge.Teece’s(1986) appropriability arguments address organisational uncertainties(i.e.even if a new product or process is technologically and commercially viable,there is no guarantee that the innovator will appropri-ate the benefits of the innovation).Synthesising these concepts,Afuah(1998)and Afuah and Bahram(1995)expanded the scope of this quest by arguing that innovations should also be viewed from the perspectives of a wider social network of suppliers,customers and complementary innova-tors.They give several examples of how compe-tency destroying attributes for customers, suppliers or complementary innovators can have detrimental affects on a new product or process, and that the key to innovative success is to minimise the disruption within the innovation value-added chain.Although an important con-tribution,it is incomplete in that it does not look beyond the innovation value-added chain mem-bers but concentrates only on what FreemanJeremy K.Hall and Michael J.C.Martin274R&D Management35,3,2005r Blackwell Publishing Ltd.2005(1984)terms the primary stakeholders of thefirm. We argue that the disruptive implications of such innovations must also be considered from the perspective of secondary stakeholders,particu-larly those innovations that,while likely to offer substantial benefits,may also introduce potential new adverse environmental,health and social side affects.3.Radical technology development andstakeholder managementAccording to Freeman(1984)stakeholders are ‘any group or individual who can affect or is affected by the achievement of thefirm’s objec-tives’(p.46)and may be either primary(those that have a direct impact on thefirm)or second-ary(those that are not directly involved with the firm but may indirectly influence thefirm via primary stakeholders).Stakeholder management thus differs from conventional input–output perspectives in that it recognises that there is a two-way relationship between thefirm and its stakeholders(Donaldson and Preston,1995),and thus draws parallels with systems thinking(Senge, 1990)and complexity theory(Simon,1962,1969). An important stakeholder management issue concerns the ethical perspective taken by thefirm. Donaldson and Preston(1995)distinguish be-tween normative and instrumental approaches to stakeholder relations.The former is concerned with how managers should deal with corporate stakeholders,whereas the latter suggests that firms view their stakeholders as part of an envir-onment that must be managed in order to assure shareholder value.Phillips et al.(2003)makes the distinction between‘normative stakeholders’, those to whom the organisation has a direct moral obligation,such asfinanciers,employees, customers,suppliers,and local communities,and ‘derivative stakeholders’,those to whom the or-ganisation has no direct moral obligation,but who can harm or benefit the organisation(e.g. competitors,activists,terrorists,the media). Jones(1995)argues that the instrumental benefits of stakeholder management cannot be disenfran-chised from a genuine commitment to ethical principles,and thatfirms that create and sustain stakeholder relationships based on mutual trust and cooperation will have a competitive advan-tage over those that do not(see also Barney and Hansen,1994;Hart and Sharma,2004).A key issue facing stakeholder management is the identification of which stakeholders are most important.Mitchell et al.(1997)argue that salient stakeholders are those that possess power(the ability of one entity to influence another),legiti-macy(socially accepted and expected behaviour), and urgency(the degree to which an issue is regarded as important or time sensitive).These attributes are of course dynamic(Beaulieu and Pasquero,2002;Elias et al.,2004),changing the importance of which stakeholders are important at any given time.Others have recognised that there are different types of stakeholders,and that these differences play an important role in how firms should engage with them.For example, Clarkson(1995)argues that stakeholders have different‘interests,claims or rights’.He narrows Freeman’s primary/secondary stakeholder defini-tion by arguing that the former are those that the corporation needs to survive,such as investors, employees,customers,suppliers,governments and communities that provide infrastructures and markets.Secondary stakeholders can influ-ence,affect(or be influenced or affected by)the firm,but are not engaged in transactions with the corporation and are not essential for its survival. Post et al.(2002)further delineate stakeholder distinctions by characterising them as falling within a corporate,resource base(customers and users,investors,employees),industry struc-ture(regulators,supply chain members,alliance partners)and social–political setting(private or-ganisations,governments,local communities and citizens).They argue that the management of ‘critical’stakeholder relations,a potential core competence for management,can involve stake-holders from all categories.Empirical studies by Berman and Wicks(1999)support the importance of effective stakeholder management:‘Managers not currently considering the effects of decisions regarding resource allocations vis-a-vis key stake-holders are at a competitive disadvantage to those whose thinking is more holistic’(p.503). Tipping et al.(1995)have explicitly recognised the importance of stakeholder analysis in technol-ogy development by noting that there is a var-iance in interests and perspectives among R&D stakeholders.More recently,Elias et al.(2002) recognise the importance of secondary stake-holders,arguing that R&D projects affect differ-ent stakeholders in different ways.They suggest an eight-stage analysis for R&D projects that includes the identification and mapping of key issues and stakeholders as well as the potentially dynamic nature of stakeholder salience.Drawing on the innovation literature discussed above,Hall and Vredenburg(2003)make the distinction be-Disruptive technologies,stakeholders and the innovation value-added chain r Blackwell Publishing Ltd.2005R&D Management35,3,2005275tween stakeholders within the innovation value-added chain and those that are not.They argue that innovation value-added chain stakeholders have similar interests,claims and rights,whereas those outside of the innovation value-added chain are often motivated by different values and ob-jectives,and thus more ambiguous in that they are often difficult to identify as salient,or present conflicting pressures that cannot be reconciled through traditional market transactions.For this reason,they argue that traditional innovation management techniques are often inadequate for dealing with secondary stakeholder pressures. Stakeholder analysis is crucial for radical tech-nology development because it may have wide-spread social and environmental implications that are often controversial(cf.Lipsey and Bekar, 1995;Freeman and Soete,1997).Nelson and Winter(1982)for example argue that technical change leads to human progress,but is uneven among economies and industries,and generates new externalities that must be later dealt with in some way or another.Because of these wide-ranging affects,radical technologies often affect, or are influenced by both primary and secondary stakeholders.Yet the innovation literature fo-cuses only on primary(innovation value-added chain)stakeholders,even though secondary stakeholders such as advocacy groups(e.g.envi-ronmental,anti-globalisation,safety,etc.),inter-governmental organisations,local communities, etc.are playing an increasingly important role in business activities(Waddock et al.,2002;Hall and Vredenburg,2003).A key challenge in radical technology development is the ability to accom-modate such pressures.That is,to exploit the potential benefits as well as assess,manage and minimise the perceived or unforeseen unintended negative consequences of the technology.A wider stakeholder analysis brings the addi-tional challenges of greater complexity and plexity,a situation where there are many interacting variables,may lead to compro-mises or less than ideal options as managers may not fully understand how all variables interact (Simon,1962,1969;Franken,2001;Gatignon et al.,2002).The recognition of more stakeholders thus means that there are that many more inter-acting variables that must be addressed.Accord-ing to Hall and Vredenburg(2003),stakeholder ambiguity is a situation where various stake-holders with disparate goals,demands and opi-nions interpret the same situation differently. They argue that ambiguity can emerge when for example a technology is based on a new science that has yet to be fully accepted(e.g.biotechnol-ogy),or simply because stakeholders have irre-concilable differences based on ethical,religious, cultural or social issues.For example,those within the innovation value-added chain are more likely to have similar‘interests,claims or rights’(Clarkson,1995)because of common goals and pressures associated with a market relation-ship,and have a clear concept of what is expected from the relationship.In contrast,those outside of the innovation value-added chain may have quite dissimilar goals and their behaviour mod-erated by non-market concerns and pressures. Complexity thus requires greater resources to figure out what is going on,whereas stakeholder ambiguity recognises that some stakeholders play by different rules.The inclusion of a broader stakeholder analysis within the innovation value-added chain ap-proach is a useful means by which managers can identify potentially disruptive affects of their radical technology.However,we also suggest that,in addition to identifying salient stake-holders,it is also important to recognise that some stakeholders have differing objectives,and that these variances can lead to conflicting goals. We thus expand on the work of Elias et al.(2002) by arguing that managers need different techni-ques for managing stakeholders depending on their relationship with the innovator.Figure1is an illustration of the innovation value-added chain and secondary stakeholders concerning the development of radical technol-ogy.In the upper left corner is the original Afuah innovation value-added chain model concerned with the degree to which an innovation affects the technological competencies of customers,suppli-ers and complementary innovators.Other pri-mary stakeholders include investors and government entities(e.g.regulators,trade minis-tries,industry promoters,etc).Governments and regulators perhaps straddle both the primary and secondary category as they can directly influence the innovatingfirm and other innovation value-added chain members.The scientific community may also represent both primary and secondary stakeholders,depending on whether or not the innovatingfirm sponsors the research.On the right are examples of secondary stakeholders that may play a disruptive role in radical technol-ogy development by presenting political chal-lenges and creating difficulties and/or disrupting the competencies of innovation value-added chain stakeholders.In contrast to innovation value-added chain stakeholders,the identification ofJeremy K.Hall and Michael J.C.Martin276R&D Management35,3,2005r Blackwell Publishing Ltd.2005salient secondary stakeholders is often elusive (hence the ‘?’stakeholder box).Furthermore,in many cases there may be contradicting pressures (Hall and Vredenburg,2003).For example,ad-vocacy groups may be opposed to a radical technology because of its potentially uncertain health and safety side effects.Conversely inves-tors may not be willing to fund large-scale re-search (with little or no potential for short-term gains)if the technology is only going to have incremental implications on an industry.4.Controversial technology development:the case of MonsantoWe now illustrate our framework of the innova-tion value-added chain for radical technology development with US-based Monsanto’s cele-brated attempts to apply the revolutionary science of biotechnology to agriculture.Of interest is the fact that they were able to satisfy the demands of regulators and most stakeholders within the in-novation value-added chain,but they encoun-tered considerable opposition with disruptive effects,mostly from secondary (non-innovation value-added chain)stakeholders.Genetic technology can allow the design of living organisms through DNA manipulation to produce crops with more marketable character-istics,such as faster growth,higher yields,better taste qualities,etc.Proponents claim that it offers a wide range of environmental and social benefits by reducing pesticide,herbicide,water and soil quality requirements (Hart,1997;Hart and Mil-stein,1999).Monsanto,a former chemicals andnow life sciences company,was a pioneer of the technology.One of their main products is Round-up s herbicide,which kills all plants except those bio-engineered to resist it,which in turn are proprietary products of Monsanto.Farmers that buy Monsanto’s ‘Round-up s ready’bio-engi-neered seeds thus need only one herbicide.The then CEO of Monsanto,Robert Shapiro,argued that biotechnology allows more efficient produc-tion by replacing ‘stuff with information’(Ma-gretta,1997).He claims that up to 90%of conventional herbicides sprayed on crops are wasted.By genetically designing products to act only on targeted problems such as specific pests and weeds,efficiencies are greatly increased.In theory,farmers would find this system attractive as it offers a lower cost-to-output ratio and allows farmers to use herbicides in a more precise way.From an innovation value-added chain perspective,farmers would see this as an incremental innova-tion as it allows them to improve their business operations without disrupting their competences.The potential of this technology appeared to be both financially and environmentally promising.However,a number of academics and advocacy groups were,for example,concerned about the long-term health implications,the risk of gene transfer from GMOs to wild plants,the unin-tended migration of GM seeds,the risk of re-duced gene pools and thus increased vulnerability because of mono-crop techniques,and the possi-bility of increased pest resistance (Hall and Crowther,1998;Goldsmith,2002;Leiss,2002).Other stakeholders were concerned about the social implications.For example,farmers may become increasingly dependent upon seedRange of StakeholdersRelatively Low Stakeholder Complexity and AmbiguityRelatively High Stakeholder Complexity and AmbiguityPrimary StakeholdersSecondary StakeholdersIVC Stake-holdersFigure 1.The innovation value-added chain (IVC),secondary stakeholders and radical technology development.Disruptive technologies,stakeholders and the innovation value-added chainr Blackwell Publishing Ltd.2005R&D Management 35,3,2005277companies,many of whom are located in devel-oping countries.This issue became quite conten-tious when Monsanto attempted to introduce the so-called terminator gene,or one that made seeds sterile,thus preventing the spread of maverick GMOs and cross-fertilisation with other species. In the past,seed companies had the rights tofirst generation seeds,but not the following genera-tions,so farmers could then generate their own. With the sterile genetically modified seeds,farm-ers must continually purchase from the seed companies,increasing their dependence on the latter and relinquishing control over breeding. The loss of tacit farming techniques such as seed breeding may never be recovered and Third World farmers will become increasingly depen-dent upon Western seed companies,thereby in-creasing the wealth gap between rich and poor nations(Charles,2001).Monsanto decided to drop the terminator gene after considerable pres-sure from social and environmental advocates (Graham,2001).The terminator gene illustrates the difficult paradoxes often faced by innovators.Monsanto must try to balance the demands of economic risk (protect their intellectual investments and receive afinancial return),environmental risk(attempt to prevent the spread of unwanted GMOs)and social risk(farmers in the Third World becoming increasingly dependent upon developed nations). This situation generates considerable variance in stakeholder perceptions that cannot be appeased without provoking others.Secondary stake-holders such as representatives of developing nations and anti-globalisation advocates per-ceived the technology as a disruptive social force, and in turn attempted to disrupt the innovation value-added chain by demanding the right for consumers to choose GMO-free bel-ling and the right of consumer choice became particularly contentious in1997when the US Food and Drug Administration(FDA)estab-lished a policy that genetically engineered soy-beans did not have to be separated at source.This was welcomed by the agricultural biotechnology industry,which argued that GMOs were funda-mentally equivalent to natural bel-ling implies that there is something potentially dangerous,and thus increases consumer resis-tance.As a major component of processed food, producers rather than end consumers primarily purchase soybeans.The FDA policy essentially meant that food producers did not have to label their products to indicate whether(or not)they contained GMOs.This conflicted with the pre-dominantly European view that all food contain-ing GMOs should be clearly labelled.Stiff resistance from consumers led to considerable pressure and public protest by Greenpeace con-cerning the sale of unlabelled soy products.This pressure was not exclusively targeted at Mon-santo,but also food producers and European supermarket chains.Soon afterwards the Eur-opean Parliament ruled that in the future all newly approved genetically modified foodstuffs must be labelled,although products already approved could continue to be unlabelled(Buck-ley,1997).Reflecting consumer concerns,some European governments refused to accept the latter concession and in Britain,some super-market chains and restaurants started GMO-free policies.Like the terminator gene,the labelling issue is another case of how secondary stakeholders can disrupt the innovation value-added chain.An original attractive attribute of the technology was that it offered products that had the same processing properties as non-GMO products,thus avoiding any disruption for complementary inno-vators like food belling disrupted the food producers and forced them tofind GMO-free sources,such as Brazil(Reuters,March20, 2003).It also gave consumers the perception that there was indeed a difference between genetically modified and traditional products.By1999there was widespread opposition to GMOs in Europe and developing countries, which was stimulated by increased awareness generated by the media,environmental pressure groups and the ability to spread information quickly via the Internet.Anti-GMO sites sprang up to challenge the scientific facts,most of which they argued were generated by the industry to promote the industry’s interests.The European offices of Monsanto were under considerable pressure to deal with an increasingly hostile pub-lic,to a point where they were starting to avoid public discussions.In2000Monsanto underwent major restructuring,including a change in com-pany leadership and ownership,with some ar-guing that it was because of the poor handling of stakeholder concerns(Barrett,2000;Champy, 2000).They also established thefive-part‘New Monsanto Pledge’that involves greater secondary stakeholder participation.Post et al.(2002)argue that if they fail to meet the pledge,they may very well fail as a company.As of2002,Monsanto continued to suffer,with the abrupt resignation of Shapiro’s replacement,Hendrick Verfaillie(Econ-omist,December21,2002).Jeremy K.Hall and Michael J.C.Martin278R&D Management35,3,2005r Blackwell Publishing Ltd.20055.Radical technology development andPopper’s approaches to science andsocietyWe suggested that radical technology develop-ment could be viewed as a new knowledge quest requiring the reduction of technological,commer-cial,organisational and social uncertainties.Be-cause of its potentially wide-ranging implications, radical technology development may be contro-versial and therefore may be disruptive to a broader range of stakeholders than only those suggested by the innovation value-added chain. Like Elias et al.(2002)and Post et al.(2002),we therefore argue that a more comprehensive sta-keholder analysis,one that includes secondary stakeholders,is thus required.However,we ex-pand on these authors’work by recognising that, because of conflicting and or contradicting goals, interests and interpretations,different techniques for addressing non-innovation value-added chain stakeholders may be needed.Given that these are for the most part philosophical differences,we suggest two approaches derived from Popper’s work on the philosophy and methodology of science.5.1.Popper’s conjecture–refutationapproachUnder relatively non-complex circumstances,i.e. where there are relatively few stakeholders and little or no stakeholder ambiguity,Popper’s (1959)‘trial-and-error-elimination learning’or ‘conjecture–refutation’approach to the evolution of scientific knowledge is appropriate(Hall and Martin,2003).His methodology is widely ac-cepted in research communities and is a common approach to managerial decision-making(cf. Martin,1983,1994;Harper,1996;Dudik,2000, Kaplan and Norton,2001).Such rational ap-proaches are implicitly reflected in such influential writers as Ansoff(1957),Andrews(1971),and Porter(1980).Thus,Popper’s‘trial-and-error-elimination learning’approach may be applied at three levels,within the innovatingfirm and its associated innovation value-added chain:1.Technological uncertainty:It must be demon-strably feasible(i.e.withstand refutations) technologically,based upon corporate scienti-fic and technological competencies.mercial uncertainty:It must be demonstra-bly feasible commercially,especially since the highest status of any technological innovationis an‘as yet unrefuted product’(Martin,1994;Harper,1996).For operations,customers, suppliers,etc.stakeholder ambiguity is com-paratively low as decisions are based on eco-nomic merit.anisational uncertainty:The developmentand exploitation of the technology should be congruent with the overall strategy and cap-abilities of thefirm.Overall Monsanto was successful in overcom-ing the technological,commercial and organisa-tional hurdles.They were able to meet regulatory approval,a key technological hurdle.They were also able to market their technically sophisticated products to farmers,a critically salient stake-holder group,who generally lacked an under-standing of biotechnology.Since the output from their products had no apparent differences than non-GM products,there was no apparent reason to believe that complementary innovators or end consumers would be disrupted.Monsanto was successful in making the difficult transition from a chemicals to a bioscience company(a potentially competency destroying innovation) via in-house development and strategic alliances (McDonald and Cadot,2001).They possessed the requisite competencies and strong intellectual property protection as suggested by Teece (1986).As of2003,there was in fact a black market for their products in GMO-banned Brazil (Reuters,March20,2003),a testament to its technological and commercial appeal.So what went wrong?We argue that Monsanto failed to appreciate that economic,technical and regula-tory pressures are not the only constraints that can hinder the development of a new technology or innovation.5.2.Popper’s piecemeal engineeringapproachPopper’s conjecture refutation approach is only valid if appropriate error-elimination criteria are applicable,which becomes increasingly unlikely under high degrees of complexity and stakeholder ambiguity(Hall and Martin,2003).This can be true when we look at controversial radical tech-nology development at the fourth societal level, where another approach is required.Popper (1945)recognised similar issues when he turned his attention to political and social(as against natural)science matters.Perceiving the threats of communism,fascism and other examples of uto-pian social engineering to society and humanDisruptive technologies,stakeholders and the innovation value-added chain r Blackwell Publishing Ltd.2005R&D Management35,3,2005279。

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