SD1-11-12-TP2[1]
MT6260 GPIO table_V1.0
VDD_EMI VDD_EMI VDD_EMI VDD_EMI VDD_EMI VDD_EMI VDD_EMI VDD_EMI VDD33 VDD33 VDD33 VDD33 VDD33 VDD33 VDD33 VDD33 VDD33 VDD33 VDD33 VDD33 VDD33
LPA0 LPCE0_B Design output high LSCE1_B Design output high LPCE1_B Design output high LSRSTB WATCHDOG LPTE LPRSTB CMDAT0 CMDAT1 CMDAT2 CMDAT3 CMDAT4 CMDAT5 CMDAT6 CMDAT7 CMHREF CMVREF CMPDN(SF voltage) (HW trap) 0:1.8V 1:3.0V CMMCLK CMPCLK CMRST(USB 2vcom) (HW trap) 0: disabled(default) 1: enabled EDIDAT
VDD33
VDD33
VDD33
BPI_BUS3 Design output low 00: X (BPI_BUS1 , BPI_BUS3 ) 01 : KEYPAD (BPI_BUS1 , BPI_BUS3 ) 10: MSDC (BPI_BUS1 , BPI_BUS3 ) 11 :CAM (BPI_BUS1 , BPI_BUS3 )
EINT5 (MCINS)
PD
PU HIGH PD PD PD PD PD PD PD PD PD PD PD PD PD PD PD
MCCK MCDA0 MCCM0 NLD8 NLD7 NLD6 NLD5 NLD4 NLD3 NLD2 NLD1 NLD0 LWRB LRDB
Fast Mutagenesis System
FM111-01 (5 rxns) 20 units 100 µl 15 µl 50 µl 6 µl 5 µl 5 µl
5 支 (50 µl/支)
FM111-02 (20 rxns) 60 units 400 µl 50 µl 50 µl 22 µl 25 µl 25 µl
20支 (50 µl/支)
PCR 体系 Control Plasmid Control Primers 5×TransStart FastPfu buffer 10 mM dNTPs TransStart FastPfu DNA Polymerase ddH2O
1-5 ng 1µl
10 µl 1µl 1µl
to 50 µl
Fast Mutagenesis System
中 (在感受态细胞刚刚解冻时加入产物),轻弹混匀,冰
浴30 分钟。
b. 42℃准确热激45秒,立即置于冰上2 min。
c. 加250 µl平衡至室温的SOC,225转,37℃培养1小时。
d. 取200 µl菌液铺板,培养过夜(为得到较多的克隆,
4000 rpm 离心1min,弃掉部分上清,保留100-150 µl,
TransGen Biotech Order Line: 010-51296890 Customer Service: 010-83011923 E-mail : tr System
目录号:FM111 保存: DMT Chemically Competent Cell -70℃保存六个月,其它-20℃保存一年。 试剂盒组成
Mutagenic Forward primer: Mutagenic Reverse primer:
注意:质粒大小大于4 kb,dNTPs 使用量为2 µl。
乐鑫ESP-WROVER-KIT开发板原理图
PWREN# SUSPEND#
10
1 5 11 15 25 35 47 51
R37/0R :ON 32K-CLK:R36/0R、 R38/0R、 R39/0R :OFF
GPIO:R36/0R、 R37/0R :OFF R38/0R、 R39/0R :ON
GND FT_GPIO3 GND FT_GPIO2 FT_GPIO1 VDD1V8 VDD1V8 VDD1V8 VDD33 VDD33 VDD33 VDD33 FT_GPIO0 C15 C16 C17 C18 C19 C20 C21 GND R40 R41 R42 2K(1%) 2K(1%) 2K(1%) D10 1 D11 1 D12 1 RED LED 2 RED LED 2 RED LED 2 R39 2K(1%) D9 1 RED LED 2
7 8
DM DP FT2232HL
D3 D4 GND VDD33 R4 R5 12K(1%) REF 1K(1%) RESET# 6 14 REF RESET#
FT_GPIO0 FT_GPIO1 FT_GPIO2 FT_GPIO3 TXD RXD nRTS nCTS nDTR nDSR nDCD nRI TXDEN 2 nRXLED nTXLED nPWRSAV nPWREN nSUSPEND D6 D5 2 BLUE LED 1 R9 RED LED 1 R15
Please place near IC-Pins.
VDD33
VDD33
VDD33 R2
Micro USB 5V&USB-UART
GND
GND C5 C8
GND 4.7uF/25V 4.7uF/25V
GND VDD33 50 VDD1V8 49 VREGIN
QPCR及QRT-PCR系列产品
Invitrogen的ICFC系列产品促销1.QPCR及QRT-PCR系列产品Invitrogen公司专门为中国客户提供的定量PCR试剂盒,结合了 UDG 防止残余污染技术和SYBR® Green I 荧光染料(存在于SYBR® Green I荧光定量PCR试剂盒中),在美国接受了严格的质量监控,可提供极高灵敏度的目的序列定量检测,线性剂量低,反应浓度范围很大。
qPCR Supermix-- 即用型反应剂,专为高特异性、实时定量DNA扩增设计UDG-- 防止携带污染物,减少克隆片段假阳性结果ROX参考染料-- 适用ABI仪器的校正染料产品信息活动时间:即日起至2009年4月30日2.Gibco南美胎牛血清即日起凡优惠价¥1780购买Gibco胎牛血清500ml(目录号:C2027050)即可获赠送价值¥250现金抵用券。
您可以凭现金抵用券在英韦创津公司购买任何商品,此券有效期至2009年5月31日。
产品信息活动时间:即日起至2009年4月30日独特的采集方式:GIBCO采用无菌心脏穿刺的方式采血原装直送,避免污染:原产地采集、加工、检测、包装。
完善的质控:采集、处理、检测、运输等环节都有文件和证书。
3.Invitrogen TA Cloning克隆产品专门用于克隆Taq聚合酶扩增的PCR产物。
采用pCR载体,能产生80%以上的重组产物,90%以上重组产物都包含插入片段。
产品信息活动时间:即日起至2009年5月31日附:pCR载体优点及图谱:3’-T突出端可直接连接Taq扩增的PCR产物可选择T7或T7和Sp6启动子进行体外RNA转录和测序侧向EcoRⅠ位点的通用多接头位点方便了插入片段的切离可以选择卡那霉素或氨苄青霉素进行筛选非常简便的蓝/白克隆筛选具有M13正向和反向引物位点,方便测序4.GIBCO液体培养基系列产品创立近50年的历史,品质优秀,产品种类丰富;为了中国用户利益,特建立国内生产线;所有产品,从原材料到生产全部按照GIBCO质量标准进行,每批均送抵美国公司总部质检合格后,才在国内销售。
安捷伦产品目录
15
Real-Time PCR
16
Mx3000P QPCR System
17
Brilliant III Ultra-Fast SYBR Green QPCR and QRT-PCR Reagents
18
Brilliant III Ultra-Fast QPCR and QRT-PCR Reagents
Agilent / STRATAGENE
Agilent website: /genomics
Welgene | Agilent Stratagene
威健股份有限公司 | Stratagene 總代理
Table of Content
Table of Contents
/ XL1-Red Competent Cells SoloPack Gold Supercompetent Cells
/ TK Competent Cells Specialty Cells
/ Classic Cells / Fine Chemicals For Competent Cells
適用於 UNG 去汙染或 bisulphite
sequencing
適用於 TA Cloning
最高敏感性
取代傳統 Taq 的好選擇
-
2
威健股份有限公司 | Stratagene 總代理
PCR Enzyme & Instrument
Agilent SureCycler 8800
市場上領先的 cycling 速度和 sample 體積 10 ~ 100 μL 簡易快速可以選擇 96 well 和 384 well 操作盤 優秀的溫控設備讓各個 well 都能保持溫度的穩定 七吋的高解析度觸控螢幕讓操作上更為簡便 可以透過網路遠端操控儀器及監控儀器 Agilent 專業的技術支援可以幫助您應對各種 PCR 的問題
博士德试剂盒说明
产品货号:SA1023产品价格:290元/1/2盒(可以做80张左右的切片),550元/1盒(可以做160张左右的切片)产品编号:SA1023—山羊IgG(适于一抗为山羊多克隆抗体)SA1024—人IgG (适于人自身抗体的检测)SA1025—大鼠IgG(适于一抗为大鼠单克隆抗体)试剂的保存:4℃可保存一年,应避免冷冻。
工作原理:SABC 是专为免疫组化和其他免疫检测而设计的,用以显示组织和细胞中抗原分布。
链霉亲和素是一种从链霉菌中提取的蛋白质,分子量47000。
同亲和素一样,对生物素分子有极高的亲和力,是一般抗原抗体亲和力的一百万倍。
亲和素是一个碱性蛋白质(IP=10),经改造后可以转变成中性蛋白质。
链霉亲和素等电点接近中性,IP=6.0~6.5,对组织和细胞的非特异吸附很低,基于链霉亲和素的免疫组化方法背景很低。
SABC 即StreptAvidin—BiotinComplex,。
根据研究,SABC 大约可形成一百个左右的过氧化物酶和五十个左右的链霉亲和素所构成的复合物。
大量的酶将保证SABC 具有很高的敏感性。
SABC 兼具高敏感性,低背景和操作简便的优点。
试剂盒中内容1.正常兔血清封闭液:12ml。
用于组织切片的封闭。
2.二抗:12ml。
亲和纯化抗体,标记长臂生物素。
兔抗大鼠IgG(或IgM)或明或暗兔抗山羊IgG 或兔抗人IgG。
3.SABC:12ml。
链酶亲和素—过氧化物酶复合物。
用户自备试剂:1.粘片剂APES 或POLY-L-LYSINE(博士德公司有售)。
2.免疫组化专用PBS(pH7.2-7.6)配法:1000ml 蒸馏水中加氯化钠8.5g, Na2HPO4 2.8g,NaH2PO4 0.4g。
如果用的是含水磷酸盐,应加上分子式中水的含量。
3.0.01M 枸橼酸盐缓冲液:1000ml 蒸馏水中加枸橼酸三钠(C6H5Na3O7·2H2O)3g, 枸橼酸(C6H8O7·H2O ) 0.4g。
Vishay 集成电路 LED 产品说明书
Dome Lens SMD LEDDESCRIPTIONThe dome lens SMD LED series has been designed in a small untinted and clear molded package with lens for surface mounting as gullwing or reverse gullwing version. The VLD.1235... series is using recent ultrabright AlInGaP / Si chip technology with high luminous flux and large chip size allowing a high DC forward current up to 70 mA. PRODUCT GROUP AND PACKAGE DATA •Product group: LED•Product series: power•Package: SMD dome lens•Angle of half intensity: ± 11°FEATURES•Utilizing latest advanced AlInGaP technology•Package type: surface-mount•Package form: gullwing, reverse gullwing•Dimensions (L x W x H in mm): 2.3 x 2.3 x 2.8•High luminous flux and luminous intensity•Luminous intensity and color categorized perpacking unit•Luminous intensity ratio per packing unitI Vmax./I Vmin.≤ 1.6•ESD-withstand voltage: up to 2 kV according to JESD22-A114-B•Preconditioning according to JEDEC® level 2a•Suitable for reflow soldering according to J-STD-020•Material categorization: for definitions of compliance please see /doc?99912APPLICATIONS•Traffic signals and signs•Interior and exterior lighting•Indicator and backlighting purposes for audio, video,LCDs switches, symbols, illuminated advertising etc.VLD.1235R...VLD.1235G...PARTS TABLEPART COLORLUMINOUSINTENSITY(mcd)atI F(mA)WAVELENGTH(nm)atI F(mA)FORWARDVOLTAGE(V)atI F(mA)TECHNOLOGY MIN.TYP.MAX.MIN.TYP.MAX.MIN.TYP.MAX.VLDS1235G Super red560011 00022 4005062663063750 1.9 2.2 2.750AlInGaP on Si VLDS1235R Super red560011 00022 4005062663063750 1.9 2.2 2.750AlInGaP on Si VLDR1235G Red900014 50035 5005061962463150 1.9 2.2 2.750AlInGaP on Si VLDR1235R Red900014 50035 5005061962463150 1.9 2.2 2.750AlInGaP on Si VLDK1235G Amber900018 00035 5005061161662150 1.9 2.25 2.750AlInGaP on Si VLDK1235R Amber900018 00035 5005061161662150 1.9 2.25 2.750AlInGaP on Si VLDY1235G Yellow900018 00035 5005058358959550 1.9 2.3 2.750AlInGaP on Si VLDY1235R Yellow900018 00035 5005058358959550 1.9 2.3 2.750AlInGaP on Si VLDS1235G-08Super red560011 00022 4005062663063750 1.9 2.2 2.750AlInGaP on Si VLDS1235R-08Super red560011 00022 4005062663063750 1.9 2.2 2.750AlInGaP on Si VLDR1235G-08Red900014 50035 5005061962463150 1.9 2.2 2.750AlInGaP on Si VLDR1235R-08Red900014 50035 5005061962463150 1.9 2.2 2.750AlInGaP on Si VLDK1235G-08Amber900018 00035 5005061161662150 1.9 2.25 2.750AlInGaP on Si VLDK1235R-08Amber900018 00035 5005061161662150 1.9 2.25 2.750AlInGaP on Si VLDY1235G-08Yellow900018 00035 5005058358959550 1.9 2.3 2.750AlInGaP on Si VLDY1235R-08Yellow900018 00035 5005058358959550 1.9 2.3 2.750AlInGaP on SiNote(1)Driving the LED in reverse direction is suitable for a short term application onlyNote(1)Tolerances: ± 15 % for I V , ± 0.1 V for V F , ± 1 nm for λdNote(1)Tolerances: ± 15 % for I V , ± 0.1 V for V F , ± 1 nm for λdNote(1)Tolerances: ± 15 % for I V , ± 0.1 V for V F , ± 1 nm for λdABSOLUTE MAXIMUM RATINGS (T amb = 25 °C, unless otherwise specified) VLDS1235..., VLDR1235..., VLDK1235..., VLDY1235...PARAMETER TEST CONDITION SYMBOLVALUE UNIT Reverse voltage (1)Short term application onlyV R 5V DC Forward current T amb ≤ 60 °CI F 70mA Power dissipation P V 200mW Junction temperatureT j 125°C Operating temperature range T amb -40 to +100°C Storage temperature rangeT stg -40 to +100°C Thermal resistance junction-to-ambientMounted on PC board (pad size > 16 mm 2)R thJA325K/WOPTICAL AND ELECTRICAL CHARACTERISTICS (T amb = 25 °C, unless otherwise specified) VLDS1235G, VLDS1235R, SUPER REDPARAMETERTEST CONDITIONSYMBOLMIN.TYP.MAX.UNIT Luminous intensity (1)I F = 50 mAI V 560011 00022 400mcd Luminous flux/luminous intensity φV /I V -0.5-mlm/mcd Dominant wavelength (1)I F = 50 mA λd 626630637nm Peak wavelengthI F = 50 mA λp -639-nm Spectral bandwidth at 50 % I rel max.I F = 50 mA Δλ-18-nm Angle of half intensity I F = 50 mA ϕ-± 11-deg Forward voltage (1)I F = 50 mA V F 1.9 2.2 2.7V Reverse currentV R = 5 VI R-0.0110μAOPTICAL AND ELECTRICAL CHARACTERISTICS (T amb = 25 °C, unless otherwise specified) VLDR1235G, VLDR1235R, REDPARAMETERTEST CONDITIONSYMBOLMIN.TYP.MAX.UNIT Luminous intensity (1)I F = 50 mAI V 900014 50035 500mcd Luminous flux/luminous intensity φV /I V -0.5-mlm/mcd Dominant wavelength (1)I F = 50 mA λd 619624631nm Peak wavelengthI F = 50 mA λp -632-nm Spectral bandwidth at 50 % I rel max.I F = 50 mA Δλ-18-nm Angle of half intensity I F = 50 mA ϕ-± 11-deg Forward voltage (1)I F = 50 mA V F 1.9 2.2 2.7V Reverse currentV R = 5 VI R-0.0110μAOPTICAL AND ELECTRICAL CHARACTERISTICS (T amb = 25 °C, unless otherwise specified) VLDK1235G, VLDK1235R, AMBERPARAMETERTEST CONDITIONSYMBOLMIN.TYP.MAX.UNIT Luminous intensity (1)I F = 50 mAI V 900018 00035 500mcd Luminous flux/luminous intensity φV /I V -0.5-mlm/mcd Dominant wavelength (1)I F = 50 mA λd 611616621nm Peak wavelengthI F = 50 mA λp -622-nm Spectral bandwidth at 50 % I rel max.I F = 50 mA Δλ-18-nm Angle of half intensity I F = 50 mA ϕ-± 11-deg Forward voltage (1)I F = 50 mA V F 1.9 2.25 2.7V Reverse currentV R = 5 VI R-0.0110μANote(1)Tolerances: ± 15 % for I V , ± 0.1 V for V F , ± 1 nm for λdNote•Wavelengths are tested at a current pulse duration of 25 ms and an accuracy of ± 1 nmNote•Luminous intensity is tested at a current pulse duration of 25 ms and an accuracy of ± 15 %.The above type numbers represent the order groups which include only a few brightness groups. Only one group will be shipped on each reel (there will be no mixing of two groups on each reel).In order to ensure availability, single brightness groups will not be orderable.In a similar manner for colors where wavelength groups are measured and binned, single wavelength groups will be shipped on any one reel. In order to ensure availability, single wavelength groups will not be orderableOPTICAL AND ELECTRICAL CHARACTERISTICS (T amb = 25 °C, unless otherwise specified) VLDY1235G, VLDY1235R, YELLOWPARAMETER TEST CONDITIONSYMBOLMIN.TYP.MAX.UNIT Luminous intensity (1)I F = 50 mAI V 900018 00035 500mcd Luminous flux/luminous intensity φV /I V -0.5-mlm/mcd Dominant wavelength (1)I F = 50 mA λd 583589595nm Peak wavelengthI F = 50 mA λp -591-nm Spectral bandwidth at 50 % I rel max.I F = 50 mA Δλ17nm Angle of half intensity I F = 50 mA ϕ± 11deg Forward voltage (1)I F = 50 mA V F 1.9 2.3 2.7V Reverse currentV R = 5 V I R0.0110μACOLOR CLASSIFICATIONGROUPDOMINANT WAVELENGTH (nm)AMBERYELLOWMIN.MAX.MIN.MAX.26116163616621583586458658955895926592595LUMINOUS INTENSITY CLASSIFICATIONGROUP LUMINOUS INTENSITY (mcd)STANDARDMIN.MAX.DB 56007100EA 71009000EB 900011 200FA 11 20014 000FB 14 00018 000GA 18 00022 400GB 22 40028 000HA28 00035 500TYPICAL CHARACTERISTICS (T amb = 25 °C, unless otherwise specified)Fig. 1 - Maximum Permissible Forward Current vs.Ambient TemperatureFig. 2 - Relative Luminous Intensity vs. Angular DisplacementFig. 3 - Forward Current vs. Pulse LengthFig. 4 - Relative Intensity vs. WavelengthFig. 5 - Forward Current vs. Forward VoltageFig. 6 - Relative Luminous Intensity vs. Forward Current0.000.020.040.060.080.100.12t p - Pulse Length (s)I F - F o r w a r d C u r r e n t (A )10-510-410-310-210-110010110217044Fig. 7 - Change of Forward Voltage vs. Ambient TemperatureFig. 8 - Relative Luminous Intensity vs. Ambient TemperatureFig. 9 - Change of Dominant Wavelength vs. Ambient TemperaturePACKAGE DIMENSIONS in millimeters: VLD.1235G.. (gullwing)PACKAGE DIMENSIONS in millimeters: VLD.1235R.. (reverse gullwing)TAPING AND REEL DIMENSIONS in millimeters: VLD.1235G (gullwing)TAPING AND REEL DIMENSIONS in millimeters: VLD.1235R (reverse gullwing)TAPING AND REEL DIMENSIONS in millimeters: VLD.1235G-08 (gullwing)TAPING AND REEL DIMENSIONS in millimeters: VLD.1235R-08 (reverse gullwing)COVER TAPE PEEL STRENGTH According to DIN EN 60286-30.1 N to 1.3 N300 ± 10 mm/min165° to 180° peel angle LABELStandard bar code labels for finished goodsThe standard bar code labels are product labels and used for identification of goods. The finished goods are packed in final packing area. The standard packing units are labeled with standard bar code labels before transported as finished goods to warehouses. The labels are on each packing unit and contain Vishay Semiconductor GmbH specific data.SOLDERING PROFILEFig. 10 - V ishay Lead (P b)-free Reflo w Soldering Profile(according to J-STD-020)BAR CODE PRODUCT LABEL (example)A.2D barcodeB.PartNo = Vishay part numberC.QTY = QuantityD.SelCode = selection code (binning)E.PTC = Code of manufacturing plantF.Batch = date code: year / week / plant codeG.Region codeH.SL = sales locationI.Terminations finishingK.Lead (Pb)-free symbolL.Halogen-free symbolM.RoHS symbolDRY PACKINGThe reel is packed in an anti-humidity bag to protect the devices from absorbing moisture during transportation and storage.FINAL PACKINGThe sealed reel is packed into a cardboard box. A secondary cardboard box is used for shipping purposes. RECOMMENDED METHOD OF STORAGEDry box storage is recommended as soon as the aluminum bag has been opened to prevent moisture absorption. The following conditions should be observed, if dry boxes are not available:•Storage temperature 10 °C to 30 °C•Storage humidity ≤ 60 % RH max.After more than 672 h under these conditions moisture content will be too high for reflow soldering.In case of moisture absorption, the devices will recover to the former condition by drying under the following condition: 192 h at 40 °C + 5 °C / - 0 °C and < 5 % RH (dry air / nitrogen) or96 h at 60 °C + 5 °C and < 5 % RH for all device containersor24 h at 100 °C + 5 °C not suitable for reel or tubes.An EIA JEDEC standard JESD22-A112 level 2a label isincluded on all dry bags.Example of JESD22-A112 level 2a labelESD PRECAUTIONProper storage and handling procedures should be followedto prevent ESD damage to the devices especially when theyare removed from the antistatic shielding bag. Electrostaticsensitive devices warning labels are on the packaging.VISHAY SEMICONDUCTORS STANDARDBAR CODE LABELThe Vishay Semiconductors standard bar code labels areprinted at final packing areas. The labels are on eachpacking unit and contain Vishay Semiconductors specificdata.FG HI MLKLegal Disclaimer Notice VishayDisclaimerALL PRODU CT, PRODU CT SPECIFICATIONS AND DATA ARE SU BJECT TO CHANGE WITHOU T NOTICE TO IMPROVE RELIABILITY, FUNCTION OR DESIGN OR OTHERWISE.Vishay Intertechnology, Inc., its affiliates, agents, and employees, and all persons acting on its or their behalf (collectively,“Vishay”), disclaim any and all liability for any errors, inaccuracies or incompleteness contained in any datasheet or in any other disclosure relating to any product.Vishay makes no warranty, representation or guarantee regarding the suitability of the products for any particular purpose or the continuing production of any product. To the maximum extent permitted by applicable law, Vishay disclaims (i) any and all liability arising out of the application or use of any product, (ii) any and all liability, including without limitation special, consequential or incidental damages, and (iii) any and all implied warranties, including warranties of fitness for particular purpose, non-infringement and merchantability.Statements regarding the suitability of products for certain types of applications are based on Vishay’s knowledge of typical requirements that are often placed on Vishay products in generic applications. Such statements are not binding statements about the suitability of products for a particular application. It is the customer’s responsibility to validate that a particular product with the properties described in the product specification is suitable for use in a particular application. Parameters provided in datasheets and / or specifications may vary in different applications and performance may vary over time. All operating parameters, including typical parameters, must be validated for each customer application by the customer’s technical experts. Product specifications do not expand or otherwise modify Vishay’s terms and conditions of purchase, including but not limited to the warranty expressed therein.Except as expressly indicated in writing, Vishay products are not designed for use in medical, life-saving, or life-sustaining applications or for any other application in which the failure of the Vishay product could result in personal injury or death. Customers using or selling Vishay products not expressly indicated for use in such applications do so at their own risk. Please contact authorized Vishay personnel to obtain written terms and conditions regarding products designed for such applications.No license, express or implied, by estoppel or otherwise, to any intellectual property rights is granted by this document or by any conduct of Vishay. Product names and markings noted herein may be trademarks of their respective owners.© 2021 VISHAY INTERTECHNOLOGY, INC. ALL RIGHTS RESERVED。
琥珀酸脱氢酶(SDH)活性检测试剂盒说明书
琥珀酸脱氢酶(SDH )活性检测试剂盒说明书可见分光光度法注意:本产品试剂有所变动,请注意并严格按照该说明书操作。
货号:BC0950 规格:50T/48S产品组成:使用前请认真核对试剂体积与瓶内体积是否一致,有疑问请及时联系索莱宝工作人员。
试剂名称 规格 保存条件 试剂一 液体60 mL×1瓶 -20℃保存 试剂二 液体0.6 mL×1支 -20℃保存 试剂三 液体5 mL×1瓶 2-8℃保存 试剂四 液体4mL×1瓶 2-8℃保存 试剂五液体3mL×1瓶2-8℃保存溶液的配制:1、 试剂二:为易挥发试剂,用完后尽快密封,-20℃保存; 产品说明:SDH (EC 1.3.5.1)广泛存在于动物、植物、微生物和培养细胞中。
SDH 是线粒体的一种标志酶,位于线粒体内膜上的一种膜结合酶,是连接呼吸电子传递和氧化磷酸化的枢纽之一。
此外,为多种原核细胞产能的呼吸链提供电子。
SDH 催化琥珀酸脱氢生成延胡索酸,脱下的氢通过吩嗪二甲酯硫酸(PMS )传递还原2,6-二氯酚靛酚(DCPIP ),并且在600nm 处具有特征吸收峰,通过600nm 吸光度的变化,测定2,6-DCPIP 的还原速度,代表SDH 酶活性。
Succinic Acid + FAD Fumaric Acid + FADHFADH + PMS FAD + PMSH 2PMSH 2 + Dichlorophenolindophenol (600nm) PMS + Reduced Dichlorophenolindophenol 注意:实验之前建议选择2-3个预期差异大的样本做预实验。
如果样本吸光值不在测量范围内建议稀释或者增加样本量进行检测。
需自备的仪器和用品:可见分光光度计、水浴锅、台式离心机、可调式移液器、1 mL 玻璃比色皿、研钵/匀浆器、冰和蒸馏水。
操作步骤:一、样本处理(可适当调整待测样本量,具体比例可以参考文献)1. 组织样本:称取约 0.1g 组织,加入1mL 试剂一和 10μL 试剂二,用冰浴匀浆器或研钵匀浆充分研磨,4℃11000g 离心10min ,取上清,置冰上待测。
碧云天生物技术 Beyotime Biotechnology - Eltrombopag (TpoR
碧云天生物技术/Beyotime Biotechnology订货热线:400-168-3301或800-8283301订货e-mail:******************技术咨询:*****************网址:碧云天网站微信公众号Eltrombopag (TpoR激动剂)产品编号产品名称包装SF1127-10mM Eltrombopag (TpoR激动剂) 10mM×0.2mlSF1127-5mg Eltrombopag (TpoR激动剂) 5mgSF1127-25mg Eltrombopag (TpoR激动剂) 25mg产品简介:化学信息:化学名3-[(5E)-5-[[2-(3,4-dimethylphenyl)-5-methyl-3-oxo-1H-pyrazol-4-yl]hydrazinylidene]-6-oxocyclohexa-1,3-dien-1-yl]benzoic acid简称Eltrombopag别名Promacta, Revolade, SB-497115, SB 497115, SB497115 中文名伊屈泼帕化学式C25H22N4O4分子量442.47CAS号496775-61-2纯度98%溶剂/溶解度Water <1mg/ml; DMSO 26mg/ml warming; Ethanol <1mg/ml溶液配制5mg加入1.13ml DMSO,或每4.42mg加入1ml DMSO,配制成10mM溶液。
SF1127-10mM用DMSO配制。
生物信息:产品描述Eltrombopag,属于biarylhydrazone小分子,是一种thrombopoietin receptor (TpoR)的非肽类激动剂,用于治疗慢性丙型肝炎相关的血小板减少和慢性免疫性(自发的)血小板减少(ITP)。
信号通路Others靶点thrombopoietinreceptor (TpoR) ----IC50 -----体外研究含有STAT激活的IRF-1启动子和人TpoR (BAF3/IRF-1/hTpoR)调控的转染荧光素酶报告基因的小鼠BAF3细胞中,Eltrombopag表现出的半数最大有效浓度(EC50)为0.27μM。
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TP2:le scoringLe ScoringLe problème auquel on va s'intéresser est le suivant:une banque chercheàdéterminer lesquels de ses clients ne rembourseront pas les prêts qui leur ont étéaccordés.Elle dispose de données relativesàd'anciens clients dont elle sait s'ils ont rembourséleur prêt et chercheàen déduire un pro l type du client"àrisque".1Récupérer les donnéesAller sur Didel et télécharger le chier TP2.csv.Noter l'emplacement oùle chier aétésauvegardédans l'arborescence.L'extension.csv signi e"comma-separated values",c'est le format standard pour l'analyse de données(tous les tableurs peuvent exporter en.csv par exemple), ce n'est en fait rien de plus qu'un chier texte contenant nos données,les di é-rents champsétant séparés par des virgules(comma).1.Ouvrir le chier TP2.csv dans un bien y a-t-il de colonnes? De lignes?Les di érentes colonnes contiennent des renseignements sur les clients:La première colonne n'a pas de nom,elle contient des entiers qui sont des identi ants clients.La deuxième colonne est"PlusDe2AnsSansPayer",elle contient des0et des1;que signi ent ces valeurs?La troisième colonne"CreditConso",contient le rapport entre le montant des créditsàla consommation contractés par le client divisépar le mon-tant maximum auquel le client a droit comme créditàla consommation.Pouvez-vous encadrer les valeurs possibles de cette colonne?La quatrième colonne contient l'âge des clients.La cinquième colonne"RatioDettes"contient le rapport entre les men-sualités du client et son salaire mensuel.Pouvez-vous encadrer les valeurs possibles de cette colonne?La sixième colonne contient le revenu mensuel du client.La septième colonne"NbreRetards90Jours"précise le nombre de fois oùle client aétéen retard de90jours ou moins pour son remboursement.La huitième colonne précise le nombre de personnesàla charge du client (enfants,...).12Charger les données dans ROuvrir une session R ainsi que l'éditeur de manièreàpouvoir conserver votre code.2.Véri er dans quel répertoire travaille R avec la commande getwd()(wd= working directory).Si R ne travaille pas làoùvous avez sauvegardéTP2.csv il faut lui dire oùchercher,pour ce faire on utilise la commande setwd().Exemple:setwd("C:\\Documents\\DonnéesR\\TP2SD1")3.Maintenant que R sait oùchercher on peut lui faire charger nos données. R dispose d'une commande faite pour importer les chiers en.csv:read.csv(). Importer les données et les charger dans une variable data0.Quel est le type de "data0"?4.A n de véri er que l'import s'est bien passéfaire un summary()de data0. Combien data a-t-il de colonnes?De lignes?3Nettoyage des données5.Regarder attentivement les colonnes"RevenuMensuel"et"NbreDeDepen-dants"dans le summary.Que remarquez vous?Que signi e"NA"?6.Créer un dataframe data2qui contienne les lignes de data sans les lignes avec des"NA".On pourra utiliser la fonction is.na().Combien de lignes a-t'on perdu?Quelle est la proportion de lignes avec de NA dans data0?7.E ectuer un nouveau summary de data2.Remarquez-vous des incohé-rences?Pouvez vous créer data3qui ne contiennent pas ces incohérences?Com-bien de lignes a-t'on perdu au nal?Quel ratio?4Analyse de la colonne des revenusOn va commencer par installer un package.En e et on peut facilement ajou-teràR de nouvelles fonctionnalités en installant un package.8.Entrer la commande:install.packages("ggplot2").On pourra choisir France: Toulouse comme miroir.9.Créer un vecteur Revenu qui contienne les valeurs de"RevenuMensuel".10.Quelle est la moyenne des revenus?La variance?Les quartiles?Com-menter.211.Tracer la fonction de répartition(utiliser la commande ecdf pour empiric cumulative distribution function).12.Tracer un histogramme avec ggplot2,on commencera par charger la li-brairie avec la commande require(ggplot2).On utilisera alors la commande qplot(Revenu,geom="histogram").Que fairepour mieux comprendre la répartition des revenus dans la zone moyenne?5Analyse graphique de data13.On commencera par créer un dataframe dataplot qui contienne une partie seulement des données de data3àl'aide de la commande sample(utiliser l'aide).14.Tracer et commenter di érents graphiquesàl'aide de la commande qplot.Aide relativeàqplot:la syntaxe de base est la suivante qplot(x,y,data=da-taplot),qui imprime les points aux coordonnées x,y dans le jeu de données dataplot.On peut aussi modi er la couleur des points(ainsi que leur forme ouleur taille).Rentrer la commandeqplot(RevenuMensuel,RatioDettes,data=dataplot,colour=factor(PlusDe2AnsSansPayer)) Expliquer et commenter.15.Pouvez vousétablir un critère empirique distinguant les futurs mauvaispayeurs des autres?(exemple:si revenu<tant et nbrededependants>tantalors mauvais payeur)3。