Total Contents (Volume 19
Stata软件的参考手册列表说明书
TitleDescriptionThis entry describes the organization of the reference manuals.RemarksThe complete list of reference manuals is as follows:[R]Stata Base Reference ManualVolume1,A–HVolume2,I–PVolume3,Q–Z[D]Stata Data-Management Reference Manual[G]Stata Graphics Reference Manual[XT]Stata Longitudinal-Data/Panel-Data Reference Manual[MI]Stata Multiple-Imputation Reference Manual[MV]Stata Multivariate Statistics Reference Manual[P]Stata Programming Reference Manual[SVY]Stata Survey Data Reference Manual[ST]Stata Survival Analysis and Epidemiological Tables Reference Manual[TS]Stata Time-Series Reference Manual[I]Stata Quick Reference and Index[M]Mata Reference ManualWhen we refer to“reference manuals”,we mean all manuals listed above.When we refer to the reference manuals,we mean all manuals listed above except the Mata Reference Manual.When we refer to the Base Reference Manual,we mean just the three-volume Base Reference Manual,known as[R].When we refer to the specialty manuals,we mean all the manuals listed above except[R]and[I], the Quick Reference and Index.Detailed information about each of these manuals can be found online at/manuals/12intro—Introduction to base reference manualintro—Introduction to base reference manual3mmarize varlist if in weight ,optionsoptions descriptionetail display additional statisticsmeanormat use variable’s display formatsepvarlist may contain factor variables;see[U]11.4.3Factor variables.varlist may contain time-series operators;see[U]11.4.4Time-series varlists.by is allowed;see[D]by.aweight s,fweight s,and iweight s are allowed.However,iweight s may not be used with the detailoption;see[U]11.1.6weight.Items in the typewriter-style font should be typed exactly as they appear in the diagram, although they may be abbreviated.Underliningmmarize may be abbreviated su,sum,summ,etc.,or it may be spelled out completely.Items in the typewriter font that are not underlined may not be abbreviated.Square brackets denote optional items.In the syntax diagram above,varlist,if,in,weight,and the options are optional.The options are listed in a table immediately following the diagram,along with a brief description of each.Items typed in italics represent arguments for which you are to substitute variable names,observation numbers,and the like.The diagrams use the following symbols:#Indicates a literal number,e.g.,5;see[U]12.2Numbers.Anything enclosed in brackets is optional.At least one of the items enclosed in braces must appear.|The vertical bar separates alternatives.%fmt Any Stata format,e.g.,%8.2f;see[U]12.5Formats:Controlling how data are displayed. depvar The dependent variable in an estimation command;see[U]20Estimation and postesti-mation commands.exp Any algebraic expression,e.g.,(5+myvar)/2;see[U]13Functions and expressions.filename Anyfilename;see[U]11.6File-naming conventions.indepvars The independent variables in an estimation command;see[U]20Estimation and postestimation commands.newvar A variable that will be created by the current command;see[U]11.4.2Lists of new variables.4intro—Introduction to base reference manual£...£SE/Robust£...£Maximizationintro—Introduction to base reference manual5。
威斯康辛大学图书馆FRUS下载步骤
威斯康辛大学图书馆FRUS下载步骤：作者:cnziyun1.在地址栏里面输入下面这个网站链接：/cgi-bin/FRUS/FRUS-idx?type=browse&scope=FRUS.FRUS1，出现的图像如下图（图一）：2.在页面里面点击某年某卷的打开，如united States department foreign relations of the united States：diplomatic papers,1945.general:the united nations volume I(1945).出现如下界面：这时，在下载文件的存放位置建立文件夹，命名为“1945，Volume I”3.看到Contents：Contents下面有被分成各章的各部分件链接，前后各有一个[cover]。
处于顶端的[cover]，出现如下界面：4.这时看到出现了本卷的封面图片，不用理会,图像格式的也有批量下载方法,不过个人以为下载paf版本的更好一些。
看本卷黑色标题下面的，这个表示了封面文件的pdf版本的链接，在其上面右键单击鼠标，选择属性，会显示出这个文件的地址。
如下图：5.复制下地址（URL），建批量下载要用。
再返回本卷的总Content界面。
6.打开content底端的[cover]，这个是本卷的封底。
和第4步一样，看其属性，记住最后的文件序号，比如本卷总共被分为14部分，则最后的序号为14：frus.frus1945v01.i0014.pdf。
7.现在打开下载软件。
以迅雷为例。
选择新建批量下载，出现对话框如下图8.将刚才复制的文件链接地址粘贴到URL框里面，如本卷的/FRUS/EFacs/1945v01/reference/frus.frus1945v01.i0001.pdf.将i0001.pdf中的01改为（*）。
（括号一定要用半角）9.在里面填从01 到14，通配符长为2.。
10.然后点确定，出现下面的窗口11.将存储目录改为第2步命名为1945，Volume I的文件夹，点确定，就可以下载了。
不同机械化整地技术对稻田土壤理化性质和水稻产量的影响
不同机械化整地技术对稻田土壤理化性质和水稻产量的影响作者：***来源：《安徽农业科学》2021年第14期摘要 [目的]了解不同机械化整地技术对稻田土壤理化性状和水稻产量的影响，为机械化技术模式的推广和开发新型农业机械提供参考。
[方法]2017—2019年在安徽省肥东县进行田间试验，比较3种不同机械化整地方式旋耕（处理①）、深松（处理②）、深翻（处理③）对稻田0～10、10～20、20～30 cm土层土壤理化性质和水稻产量的影响。
[结果]与处理①相比，处理②和③在0～20 cm土层可导致土壤含水率降低9.67%～26.79%，土壤容重增加0.08～0.16g/cm3，且差异达到显著水平，土壤透气性有所提高，土壤孔隙度降低3.05%～11.67%;各土层总体表现出有机碳、全氮和碱解氮含量均逐渐降低，0～10 cm土层的有机质、全氮和碱解氮含量均低于处理①;处理②条件下水稻产量2年持续增加，第1、2年分别增产4.91%和9.54%，处理③对水稻产量影响较小，仅在第2年呈现出增长的趋势，但是差异不显著。
[结论]深松可以提高稻田土壤向下的透水性和容重，对土壤有机质和氮素影响较小，可连年增加水稻产量。
深翻虽然也可以改善土壤的理化性质，但是效果较深松处理差，且会降低上层土壤肥力，水稻产量增加不明显。
关键词机械化;整地技术;水稻;土壤;理化性质;产量中图分类号 S 28 文献标识码 A 文章编号 0517-6611（2021）14-0200-03Abstract [Objective] To understand the effects of different mechanized soil preparation technologies on the physical and chemical properties of paddy soil and rice yield， and to provide references for the promotion of mechanized technology models and the development of new agricultural machinery.[Method]Field trials were carried out in Feidong County， Anhui Province from 2017 to 2019. Three different mechanized land preparation methods were compared with rotary tillage （treatment ①）， subsoiling （treatment ②） and deep plowing （treatment ③） on rice fields of 0-10，10-20，20-30 cm soil physical and chemical properties and the influence of rice yield.[Result]Compared with treatment ①， treatments ② and ③ in the 0-20 cm soil layer can reduce the soil moisture content by 9.67% to 26.79%，soil bulk density increased by 0.08-0.16 g/cm3， and the difference reached a significant level， soil air permeability was improved， and soil porosity decreased by 3.05%-11.67%，the overall soil layers showed that the contents of organic carbon，total nitrogen and alkali hydrolyzed nitrogen gradually decreased， and the contents of organic matter， total nitrogen and alkali hydrolyzed nitrogen in the 0-10 cm soil layer were all lower than treatment ①;under the condition of treatment ②， the rice yield increased continuously for 2 years，and the yield increased by 4.91% and 9.54% in the first and second years respectively. Treatment ③had little effect on rice yield， and showed an increasing trend only in the second year， but the difference was not significant.[Conclusion]Subsoiling can increase the downward water permeability and bulk density of the paddy soil， and has little effect on soil organic matter and nitrogen， and can increase rice yield year after year.Although deep plowing can also improve the physical and chemical properties of the soil， it is less effective than deep loosening， and will reduce the fertility of the upper soil， and the increase in rice yield is not obvious.Key words Mechanization;Land preparation technology;Rice;Soil;Physical and chemical properties;Yield作者简介周秀梅（1976—），女，安徽合肥人，工程师，从事农业机械监管、农业机械新技术推广等工作。
iLoud Micro Monitor 用户手册说明书
Studio reference monitor USER MANUALEnglishPortuguês中文日本語ContentsT able of ContentsContents 2 English 4 iLoud Micro Monitor 4 Register your iLoud Micro Monitor 4 iLoud Micro Monitor overview 5 Installation and setup 6 Control panel 10 Audio inputs 10 Speaker’s connection ports 10 Bluetooth pairing 11 Volume 11 Power switch and DC input 11 Acoustic setup 12 Desktop filter 12 High frequency filter 13 Low frequency filter 13 LED 13 Drivers 13 Woofer 13 Tweeter 13 Bass port 14 Speakers placement 14 Desktop positioning 14 Listening position 15 Room acoustics 15 Microphone stand mounting 16 Block diagram 16 Troubleshooting 17 Specifications 17 Graphics 18 Warranty 1 9 Support and more info 19 Português 20 iLoud Micro Monitor 20 Registre seu iLoud Micro Monitor 20 Visão Geral do iLoud Micro Monitor 21 Instalação e configuração 22 Painel de controle 26 Entradas de áudio 26 Portas de conexão entre os monitores 26 Emparelhamento Bluetooth 26 Volume 27iLoud Micro MonitorThank you for purchasing iLoud Micro Monitor.Y our package contains• iLoud Micro Monitor speakers• Power supply unit• Speakers connection cable• RCA cable• Quick start guide• Registration cardIt’s a pair of portable studio monitors with a professional-quality bi-amplified design that provides an honest and accurate sound with a true linear frequency response.iLoud Micro Monitor is powered by four amps that deliver a combined 50 W RMS of power. Each offer extreme clarity and superior bass response via two 3/4” tweeters and two 3” woofers.These features are augmented by an on-board 56-bit DSP processor. It gives you total control of the monitor’s performance, allowing for stunningly accurate and detailed sound that’s hardly believable considering the size and price.It can be adjusted to suit different playback situations thanks to its speakers’ two positionable angles of inclination and three dedicated EQ switches: bass, treble and a “free field”/”desktop” compensation switch. These switches let you optimize its sound for your listening environment.It also comes with a full range of source options that includes Bluetooth streaming as well as RCA and 1/8” inputs.iLoud Micro Monitor is perfect for mixing, mastering, composing, producing, critically listening and more in a wide variety of situations both while traveling and while at home or in the studio.Register your iLoud Micro MonitorBy registering, you can access technical support, activate your warranty and receive free JamPoints™ which will be added to your account. JamPoints™ allow you to obtain discounts on future IK purchases! Registering also keeps you informed of all the latest software updates and IK products.Register at: /registrationiLoud Micro Monitor overviewActive speaker (left)Installation and setupWe recommend using high-quality audio cables to guarantee optimal performance. It is important to ensure the speakers stand firmly on a solid ground. Please note that the loudspeakers will need a few days to achieve optimum sonic performance.1. Make sure that the volume control of iLoud Micro Monitor is set to minimum. Make sure that the ON/OFF®7. Play audio from your audio source and slowly raise the volume of the iLoud Micro Monitor to a comfortable8. If needed, adjust the speakers response with the dedicated controls on the rear panel of the left speaker.Note: Before plugging in and turning on, remember the “last on, first off” rule of powered speakers. When powering up your system, be sure that all the wires are connected, turn your mixer/interface and any other outboard gear on, and then last turn on your iLoud Micro Monitor. When powering down, turn your iLoud Micro Monitor off first and then your mixer/interface and outboard gear.Control panelAudio inputsRCA unbalanced line inputThese RCA jacks allows for the connection of line sources (i.e., mixer/audio interface bal/unbal line out) both for the Left and Right channel.1/8” AUX unbalanced inputThis unbalanced line input allows for the connection of stereo unbalanced audio sources (i.e., computer stereo output, music player stereo output, smartphone/tablet stereo output).Speaker’s connection portsThese ports (present on both speakers) allows you to connect the active speaker (Left port) with the passive speaker (Right one). These are 4 pins connectors: in this way is it possible to carry the powered signal both for the HF driver and the LF driver.Bluetooth pairingThe iLoud Micro Monitor uses Bluetooth® to stream high quality audio from your Bluetooth® enabled device without the need to connect any audio cable. In order for your device to work with the iLoud Micro Monitor it must first be paired. T o pairing your device:• Turn the back panel volume control counter-clockwise to the -INF position.• With the iLoud Micro Monitor and your Bluetooth® device powered on: press the PAIRING button on the rear panel of the Left speaker to make the speaker discoverable. The front panel indicator will slowly flash blue.• In the settings of your Bluetooth® device, set it to “discover” available devices.• From the Bluetooth® device list, select the “iLoud Micro Monitor” device.• iLoud Micro Monitor indicates that pairing is complete when the indicator returns steady white. Y ou can now stream audio from your device to the iLoud Micro Monitor: only when there’s an audio streaming the LED turns steady blue.• With the volume control on your Bluetooth® device turned up, play some music and slowly raise the iLoud Micro Monitor volume control until you have reached the desired level.VolumeThis potentiometer allows you to adjust the input level of the audio source.Power switch and DC inputPower switch: this rocker-style switch allows to power on and off iLoud Micro Monitor.DC IN: connect the (included) PSU to this DC socket.IMPORTANT: before to connect/disconnect the DC input, make sure that the power switch is set to Off.Acoustic setupThese three switches on the back panel will help you to fit perfectly iLoud Micro Monitor in every acoustic environment.Desktop filterThis switch sets a notch filter to compensate the typical acoustic effect of a console or a desktop. Such placement usually results in a peak in the lower midrange. The Desktop switch activates a bell shaped notch filter, with a +3.5dB between 1kHz and 10kHz and -1dB below 400Hz.High frequency filterThis switch controls the high frequency level using a shelf-type EQ. The level can be set to 0dB or +2dB (above 4kHz).Low frequency filterThis switch controls the low frequency level using a shelf-type EQ. The level can be set to 0dB or -3dB (below 250Hz).This filter is used to adjust the proximity effect of side walls and ceiling. Use this filter whenever the acoustics of your room cause problems in the low frequency range.LEDThe frontal LED on the Left speaker will show important information about iLoud Micro Monitor’s status: Steady white: iLoud Micro Monitor is ON.Steady red: clipping.Flashing blue: iLoud Micro Monitor is pairing with your Bluetooth device.Steady blue: the Bluetooth connected device is streaming audio to the speakers.DriversWooferThe woofer unit is a custom 3” paper cone capable to delivers balanced mid- and low-frequency response.T weeterBy employing a specially developed 3/4” silk cone with neodymium magnet, the tweeter can deliver an extremely natural response on the top-end.Bass portThe hole in the front panel is a bass reflex port. It is designed to discharge the low frequency signals and enhance the overall bass response.Speakers placementPlease make sure that no obstacles are presents in the way from the monitors to your ears. Y ou should be able to see the speakers completely. iLoud Micro Monitor has been designed for a vertical set up. The speakers must be positioned on a firm ground or a stand.Please note that vibrating parts of nearby objects can mask the sound.Desktop positioningThe point between the woofer and the tweeter should be positioned approximately at the height of your ears. In case you need to position the speakers in a significant lower or higher position, the monitors should be angled accordingly. If the speakers are placed lower than the ear line, you can tilt them with the integrated rubber pads that, also, improves the acoustic decoupling of the speakers from the surface.Listening positionWhen you use iLoud Micro Monitor for stereo applications, the optimum listening position should be located in the middle of an imaginary triangle. This means that your listening point (sweet point) will be located at the top of an equilateral triangle and the two loudspeakers should be placed at the other two points of this triangle. Furthermore, a symmetrical positioning is also important: this applies to the distance between speakers as well as to the walls, ceiling and floor. In order to have a symmetrical stereo image it is also important to have symmetrical reflections: we recommend to keep a distance of at least 20 cm (8 inches) from the walls, in order to avoid low frequency boosting.Room acousticsThe room acoustics plays a key role in the performance of your monitoring system. It is always advisable to make, at least, a minimum of acoustic treatment of your environment. This, along with the correct monitor’s positioning, will improve the linearity and the precision of the listening.Microphone stand mountingThanks to the thread adapter on the bottom of the speakers, iLoud Micro Monitor can be mounted on any standard 3/8” microphone stand. This allows you to place the speakers at the perfect height and, most important, helps to reduce the typical reflections of the desktop (or shell) and, consequently, improves the frequency response/linearity of the speaker.Block diagramTroubleshootingI have connected my device to iLoud Micro Monitor, but no sound comes out.Make sure to set up the volume with the volume potentiometer on iLoud Micro Monitor and/or with your device’s volume control.Sound is distorted.When a noticeable distortion occurs, please turn down the level of the speakers, or of the connected audio source.Specifications- T ype: 2-way active, bi-amped, studio monitor- Frequency response (-3dB): 55Hz - 20kHz- Frequency response (-10dB): 45 Hz - 22 kHz- Crossover Frequency: 3kHz- T weeter: 3/4” silk dome and neodymium magnet- Woofer: 3” paper cone- Maximum SPL @ 50cm (with two speakers playing, averaged sinewave from 100 Hz to 10 kHz): 107dB - Number of amplifiers: 4- Amplifiers type: Class-D- T otal output power (both speakers): 70W (peak) - 50W RMS- Woofer output power (single speaker): 18W RMS- T weeter output power (single speaker): 7W RMS- Acoustic settings: high shelf (0dB or +2dB from 4kHz up); low shelf (0dB or -3dB from 250Hz down); desktop (+3.5dB between 1kHz and 10kHz and -1dB below 400Hz)- High performance Bass-Reflex port- Bluetooth® compatible with A2DP protocol- 56-bit DSP internal resolution- System total latency (analog input): 1.02 ms- System total latency (Bluetooth input): 155 ms (depends on host implementation, tested on iPhone 6S / iOS 10.0.1)- Connectors: 2x RCA unbalanced input; 1/8” AUX stereo input (unbalanced); 4-pin speakers connector- Dimensions (inches): 180mm x 135mm x 90mm (7” x 5.31” x 3.54”)- Weight (oz): 920g (32.45oz) left speaker - 800g (28.22oz) right speakerGraphics 100M a g n i t u d e (d B )Frequency (Hz)-20-1010201K 10KILOUD MICRO MONITOR FREQUENCY RESPONSE(normalized at 0dB)Measured at 1 meter distance, in anechoic chamber, on tweeter axis.All calibration switches set to “FLAT”.100M a g n i t u d e (d B S P L )Frequency (Hz)607080901001101K 10KILOUD MICRO MONITOR MAXIMUM SPLMeasured at 50 cm distance, with two speakers playing, for 10% THD.WarrantyPlease visit:/warranty for the complete warranty policy.Support and more info /support 。
完工破舱稳性计算
图样和技术文件履历 PLAN HISTORY日期 DATE 版本 REV. 标记 MARK 数量Q’TY修改通知单号REVISE NO. 说 明 DESCRIPTION 设绘 DESIGNED 校对 CHECKED 审定 APPROVED 2005-12-23A 完工文件 黄小勇 李从波杨葆和OT1100-1 FINAL DESIGN图号 DWG.NO DL594-050-006JS标记 数量 修改单号 签字 日期 110,000吨级载重量原油船 110,000DWT Crude Oil Tanker 代码 CODE版本REV .A 重量kg MASS 比例 SCALE 设绘DESIGNED校对CHECKED共 63 页 TOTAL SHEETS 第 1 页 SHEET 审核REVIEWED完工破舱稳性计算 FINAL DAMAGE STABILITY CALCULATION 标检STANDARDIZED审定APPROVED 天鹅洲 TIAN E ZHOU 大连造船重工有限责任公司 船 舶 设 计 研 究 所 DALIAN SHIPYARD CO . , LTDSHIP DESIGN & RESEARCH INSTITUTECONTENTS1.PRINCIPAL PARTICULAR (3)1.1.G ENERAL (3)1.2.P RINCIPAL D IMENSIONS (3)2.EXPLANATION TO THE CALCULATION (3)2.1.ICLL1966/1988 (3)2.2.MARPOL 73/78 (6)3.DAMAGE CASE (8)4.RESTRICT (12)4.1.D OWN-F LOODING P OINTS (12)4.2.W EATHER-T IGHT P OINTS (12)5.RESULTS OF THE DAMAGE STABILITY CALCULATION (13)1. Principal Particular1.1. GeneralHull No.: OT1100-1CCS Con. No.: SP046069Owner: Nanjing Changjiang Oil Transportation Co.Builder: Dalian Shipyard Co., Ltd.Designer: MARIC82Navigation Area: Unrestricted Ocean-goingClass: CSA, Oil Tanker, Double Hull, F.P.≤60°C, CCSS, ESP,Ice Class B, In Water Survey, Single Point Mooring,Emergency Towing Arrangements, Loading ComputerS.I.D.CSM, AUT-0, SCM, IGS, CMSDimensions1.2. PrincipalLength over all: about 244.500 mLength, registered: 234.980 mLength between perpendiculars: 233.000 mBreadth (Moulded): 42.000 mDepth (Moulded): 21.900 mCamber: 0.780 mDesign Draught (Moulded): 12.000 mScantling (Maximum Summer) Draught (Moulded): 15.500 mSummer Freeboard (from Top of Freeboard Deck): 6.416 mDisplacement (Design/Scantling Draught): 97771/130201 tLightship Weight (assumed): 19930.4 tDeadweight (Design/Scantling Draught): about 77840.6 t/110270.6 tBlock coefficient (Design/Scantling Draught): 0.8123/0.8374Total Overall Area of Bilge Keels : 53.2m2 (Fr.118~ Fr.163)(Fr.168~Fr.208)Capacity:Cargo Oil Tanks (100%Full, including SLOP T.): 126656.7 m3Heavy Fuel Oil Tanks (100%Full, including H.F.O.Overflow T.) 3420.4 m3Diesel Oil Tanks (100%Full) 230.8 m3Lubrication Oil Tanks (100%Full) 169.9 m3Ballast Water Tanks (100%Full) 39042.1 m3Fresh Water Tanks (100%Full) 519.6 m32. Explanation to the CalculationThe proof of the damage stability is effected according to the criteria of ICLL66/1988protocol and “MARPOL 73/78” Consolidated Edition 20022.1. ICLL1966/19882.1.1 Type of ship and intended useThis ship’s purpose of freeboard computation is type A (See FREEBOARD CALCULATION, Drawing No. DL594-101-005JS), she is designed to carry liquid cargoes in bulk; she has a high integrity of exposed deck with only small access openings to cargo compartments, closed by watertight gasketed covers of steel or equivalent material; and has low permeability of load cargo compartments.The type A ship when loaded in accordance with the requirements of paragraph 2.1.2 shall be able to withstand the flooding of any compartment or compartments, with an assumed permeability of 0.95, consequent upon the damage assumptions specified in paragraph 2.1.2, and shall remain afloat in a satisfactory condition of equilibrium as specified in paragraph 2.1.3. In such a ship the machinery space shall be treated as a floodable compartment, but with a permeability 0.85.2.1.2 Initial Conditions of LoadingThe initial condition of loading before flooding shall be determined as follows:a) The ship is loaded to its summer load waterline on an imaginary even keel.b) When calculating the vertical center of gravity, the following principles apply:(i) Homogeneous cargo is carried.(ii) All cargo compartments, except those referred to under (iii) of this sub-paragraph, but including compartments intended to be partially filled, shall be consideredfully loaded except that in the case of fluid cargoes each compartment shall betreated as 98 percent full.(iii) If the ship is intended to operate at its summer load waterline with empty compartments, such compartments shall be considered empty provided the heightof the center of gravity so calculated is not less than as calculated under (ii) of thissub-paragraph.(iv) Fifty percent of the individual total capacity of all tanks and spaces fitted to contain consumable liquids and stores is allowed for. It shall be assumed that foreach type of liquid, at least on transverse pair or a single center line tank hasmaximum free surfaces is the greatest; in each tank the center of gravity of thecontents shall be taken at the center of volume of the tank. The remaining tanksshall be assumed either completely empty or completely filled, and thedistribution of consumable liquids between these tanks shall be effected so as toobtain the greatest possible height above the keel for the center of gravity.(v) At an angle of heel of not more than 5 degree in each compartment containing liquids, as prescribed in (ii) of this sub-paragraph except that in the case ofcompartments containing consumable fluids, as prescribed in (iv) of thissub-paragraph of this paragraph, the maximum free surface effect shall be takeninto account. Alternatively, the actual free surface effects may be used, providedthe methods of calculation are acceptable to the Administration.(vi) Weight shall be calculated on the basis of the following values for specific gravities:Salt Water 1.025Fresh Water 1.000Oil Fuel 0.950Diesel Oil 0.900Lubricating Oil 0.900Calculation of this initial loading condition “Load Line”INIT LOADLINET, 15.5TRIM, 0KG, 12.58GMR 1.25 (GM-reduction)OK2.1.3 Damage AssumptionsThe following principles regarding the character of the assumed damage apply:a) The vertical extent of damage in all case is assumed to be from base line upwardswithout limit.b) The transverse extent of damage is equal to B/5=8.4m (or 11.5m, whichever is thelesser), measured inboard from the side of the ship perpendicularly to the centerline at the level of the summer load waterline.c) If damage of a lesser extent than specified in sub-paragraphs a) and b) of thisparagraph results in a more severe condition, such lesser extent shall be assumed.d) The flooding shall be confined to a single compartment between adjacent transversebulkheads provided the inner longitudinal boundary of the compartment is not in aposition within the transverse extent of assumed damage. Transverse boundarybulkheads of wing tanks, which do not extend over the full breadth of the ship shallbe assumed not to be damaged, provided they extend beyond the transverse extentof assumed damage prescribed in sub-paragraph b) of this paragraph. If in atransverse bulkhead there are steps or recesses of not more than 3.00 meters inlength located within the transverse extent of assumed damage as defined insub-paragraph b) of this paragraph, such transverse bulkhead may be consideredintact and the adjacent compartment may be floodable singly. If, however, withinthe transverse extent of assumed damage there is a step or recess of more than 3.00meters in length in a transverse bulkhead, the two compartments adjacent to thisbulkhead shall be considered as flooded. The step formed by the after peakbulkhead and the after peak tank top shall not be regarded as a step for the purposeof this Regulation.e) Where the flooding of any two adjacent fore and aft compartments is envisagedmain transverse watertight bulkheads shall be spaced at least 1/3L2/3=12.69m (or14.5m, whichever is the lesser), in order to be considered effective. Wheretransverse bulkhead are spaced at a lesser distance. One or more of these bulkheadsshall be assumed as non-existent in order to achieve the minimum spacing betweenbulkheads.2.1.4 Condition of Equilibriuma) The final waterline after flooding, taking into account sinkage, heel, and trim, isbelow the lower edge of any opening through which progressive flooding may takeplace. Such openings shall in include air pipes, ventilators and openings which areclosed by means of weathertight doors or hatch covers, and may exclude thoseopenings closed by means of manhole covers and flush scuttles, cargo hatch covers,remotely operated sliding watertight doors, and side scuttles of the non-openingtype.(PROGR.LL)b) If pipes, ducts or tunnels are situated within the assumed extent of damagepenetration as defined in paragraph 2.1.3 b), arrangements are to be made so thatprogressive flooding cannot thereby extend to compartments other than thoseassumed to be floodable in the calculation for each case of damage.c) The angle of heel due to unsymmetrical flooding does not exceed 15 degrees. If nopart of the deck is immersed, an angle of heel of up to 17 degrees may beaccepted.(MAXHEEL.LL)d) The metacentric height in the flooded condition is positive.(MINGM.LL)e) When any part of the deck outside the compartment assumed flooded in particularcase of damage is immersed, or in any case where the margin of stability in theflooded condition may be considered doubtful, the residual stability is to beinvestigated. It may be regarded as sufficient if the righting lever curve hasminimum range of 20 degrees beyond the position of equilibrium(RANGE.LL)with a maximum righting lever of at least 0.1 meter within thisrange(MAXGZ.LL). The area under the righting lever curve within this range shallbe not less than 0.0175 meter-radians(MINAREA.LL). The administration shallThe following provisions regarding the extent and the character of the assumeddamage shall apply:a) Side Damage(i) Longitudinal extent 1/3L2/3=12.96m (or 14.5 m, whichever is less)(ii) Transverse extent B/5=8.4m (or 11.5 m, whichever is less) (inboard from the ship’s side at right angles to the center-line at the level of the summer load line)(iii) Vertical extent from the moulded line of the bottom shell plating atcenter-line, upwards without limitb) Bottom DamageFor 0.3L (#203+484) from the F.P. Any other part of the ship(i) Longitudinal extent 1/3L2/3=12.96 meters 5 meters(or 14.5m, whichever is less) (or 1/3L2/3, whichever is less) (ii) Transverse extent B/6= 7 meters 5 meters(or 10m, whichever is less) (or B/6, whichever is less) (iii) Vertical extent B/15=2.8 meters(or 6m, whichever is less measured from the moulded line of thebottom shell plating at centerline)c) Bottom Raking Damage(i) Longitudinal extent 0.6L=140.988m measured from F.P. (from #117+510)(ii) Transverse extent B/3=16 meters anywhere in the bottom(iii) Vertical extent breach of the outer hulld) If any damage of a lesser extent than the maximum extent of damage specified in a)and b) of this paragraph would result in a more severe condition, such damage shallbe considered.e) Where the damage involving transverse bulkheads is envisaged as specified insubparagraph 2.2.1, transverse watertight bulkheads shall be spaced at least at adistance equal to the longitudinal extent of assumed damage specified in a) of thisparagraph in order to be considered effective. Where transverse bulkheads are spacedat a lesser distance, one or more of these bulkheads within such extent of damageshall be assumed as non-existent for the purpose of determining floodedcompartments.f) If pipes, ducts or tunnels are situated within the assumed extent of damage,arrangements shall be made so that progressive flooding cannot thereby extend tocompartments other than those assumed to be floodable for each of dagame.2.2.4 Damage Stability CriteriaThis ship shall be regarded as complying with the damage stability criteria if thefollowing requirements are met:a) The final waterline, taking into account sinkage, heel and trim, shall be below thelower edge of any opening through which progressive flooding may take place. Suchopenings shall include air-pipes and those which are closed by means of watertightdoors or hatch covers and may exclude those openings closed by means of watertightmanhole covers and flush scuttles, small watertight cargo tank hatch covers whichmaintain the high integrity of the deck, remotely operated watertight sliding doors,and sidescuttles of the non-opening type.(PROGR.M)b) In the final stage of flooding, the angle of heel due to unsymmetrical flooding shallnot exceed 25 degrees, provided that this angle may be increased up to 30 degrees ifno deck edge immersion occurs.(MAXHEEL.M)c) The stability in the final stage of flooding shall be investigated and may be regardedas sufficient I the righting lever curve has at least a range of 20 degrees beyond theposition of equilibrium(RANGE.M) in association with a maximum residual rightinglever of at least 0.1m within the 20 degrees range(MAXGZ.M); the area under thecurve within this range shall not be less than 0.0175 meter radians(MINAREA.M).5. RESULT OF DAMAGE STABILITY CALCULATIONABBREVIATIONS USED IN THE OUTPUT LISTSCASE initial cond/damage case T draught, moulded m TR trim m HEEL heeling angle degree RANGE range of righting lever degree MAXGZ maximum of GZ-curve m MAXKG maximum KG m FAUN fl. angle of unprotected openings degree FAWE fl. angle of weathertight openings degree CASE initial cond/damage case RCR relevant criteria SIDE side of ship SB/PS REQ required value ATTV attained value UNIT unit STAT status of stability crit.5.1 DAMAGE STABILITY FOR ICLL TYPERESULTS---------------------------------------------------------------------- CASE T TR HEEL RANGE MAXGZ MAXKG FAUN FAWE m m degree degree m m degree degree ---------------------------------------------------------------------- IL/LL01 15.628 0.865 0.0 79.9 2.43 17.772 40.1 20.3 IL/LL02 17.128 8.793 0.0 75.1 1.70 16.806 - 8.3 IL/LL03 16.546 3.874 8.3 68.5 1.33 14.061 31.3 14.7 IL/LL04 16.458 3.360 8.4 69.9 1.42 14.494 32.5 15.9 IL/LL05 16.424 1.307 8.2 69.8 1.43 15.124 35.6 16.6 IL/LL06 16.436 -0.495 8.3 68.9 1.38 15.039 38.3 16.9 IL/LL07 16.463 -2.354 8.5 67.6 1.29 14.876 41.1 15.1 IL/LL08 16.506 -4.421 8.9 65.8 1.17 14.307 44.2 13.1 IL/LL09 16.453 -5.648 7.3 67.6 1.30 14.760 46.7 12.2 IL/LL10 15.883 -2.360 0.0 79.2 2.30 17.823 44.6 17.5 ----------------------------------------------------------------------STABILITY CRITERIA---------------------------------------------------------------------- CASE RCR SIDE REQ ATTV UNIT STAT ---------------------------------------------------------------------- IL/LL01 PROGR.LL SB 0.0000 6.5998 m OK IL/LL01 MAXHEEL.LL SB 17.0000 0.0000 deg OK IL/LL01 MINGM.LL SB 0.0000 5.2083 m OK IL/LL01 MAXGZ.LL SB 0.1000 1.9180 m OK IL/LL01 MINAREA.LL SB 0.0175 0.3306 mrad OK IL/LL01 RANGE.LL SB 20.0000 40.1243 deg OK IL/LL02 PROGR.LL SB 0.0000 1.4605 m OK IL/LL02 MAXHEEL.LL SB 17.0000 0.0290 deg OK IL/LL02 MINGM.LL SB 0.0000 4.7575 m OK IL/LL02 MAXGZ.LL SB 0.1000 1.4645 m OK IL/LL02 MINAREA.LL SB 0.0175 0.2776 mrad OK IL/LL02 RANGE.LL SB 20.0000 75.0842 deg OK IL/LL03 PROGR.LL SB 0.0000 2.3562 m OK IL/LL03 MAXHEEL.LL SB 17.0000 8.3447 deg OK IL/LL03 MINGM.LL SB 0.0000 5.6932 m OK IL/LL03 MAXGZ.LL SB 0.1000 1.2612 m OK IL/LL03 MINAREA.LL SB 0.0175 0.2758 mrad OK IL/LL03 RANGE.LL SB 20.0000 22.9367 deg OK IL/LL04 PROGR.LL SB 0.0000 2.7109 m OK IL/LL04 MAXHEEL.LL SB 17.0000 8.4008 deg OK IL/LL04 MINGM.LL SB 0.0000 5.7522 m OK IL/LL04 MAXGZ.LL SB 0.1000 1.3361 m OK IL/LL04 MINAREA.LL SB 0.0175 0.2897 mrad OK IL/LL04 RANGE.LL SB 20.0000 24.1371 deg OK IL/LL05 PROGR.LL SB 0.0000 2.9611 m OK IL/LL05 MAXHEEL.LL SB 17.0000 8.1788 deg OK IL/LL05 MINGM.LL SB 0.0000 5.7075 m OK IL/LL05 MAXGZ.LL SB 0.1000 1.3602 m OK IL/LL05 MINAREA.LL SB 0.0175 0.2985 mrad OK IL/LL05 RANGE.LL SB 20.0000 27.3969 deg OK IL/LL06 PROGR.LL SB 0.0000 3.1765 m OK IL/LL06 MAXHEEL.LL SB 17.0000 8.2596 deg OK IL/LL06 MINGM.LL SB 0.0000 5.6442 m OK---------------------------------------------------------------------- CASE RCR SIDE REQ ATTV UNIT STAT ---------------------------------------------------------------------- IL/LL07 PROGR.LL SB 0.0000 2.4445 m OK IL/LL07 MAXHEEL.LL SB 17.0000 8.5016 deg OK IL/LL07 MINGM.LL SB 0.0000 5.5918 m OK IL/LL07 MAXGZ.LL SB 0.1000 1.2463 m OK IL/LL07 MINAREA.LL SB 0.0175 0.2799 mrad OK IL/LL07 RANGE.LL SB 20.0000 32.6124 deg OK IL/LL08 PROGR.LL SB 0.0000 1.5488 m OK IL/LL08 MAXHEEL.LL SB 17.0000 8.9487 deg OK IL/LL08 MINGM.LL SB 0.0000 5.5140 m OK IL/LL08 MAXGZ.LL SB 0.1000 1.1355 m OK IL/LL08 MINAREA.LL SB 0.0175 0.2569 mrad OK IL/LL08 RANGE.LL SB 20.0000 35.2638 deg OK IL/LL09 PROGR.LL SB 0.0000 1.8294 m OK IL/LL09 MAXHEEL.LL SB 17.0000 7.2563 deg OK IL/LL09 MINGM.LL SB 0.0000 5.3629 m OK IL/LL09 MAXGZ.LL SB 0.1000 1.2409 m OK IL/LL09 MINAREA.LL SB 0.0175 0.2693 mrad OK IL/LL09 RANGE.LL SB 20.0000 39.4721 deg OK IL/LL10 PROGR.LL SB 0.0000 6.0562 m OK IL/LL10 MAXHEEL.LL SB 17.0000 0.0000 deg OK IL/LL10 MINGM.LL SB 0.0000 5.3163 m OK IL/LL10 MAXGZ.LL SB 0.1000 1.8956 m OK IL/LL10 MINAREA.LL SB 0.0175 0.3337 mrad OK IL/LL10 RANGE.LL SB 20.0000 44.6467 deg OK ----------------------------------------------------------------------5.2 DAMAGE STABILITY FOR MARPOLINITIAL CONDITION : I01RESULTS---------------------------------------------------------------------- CASE T TR HEEL RANGE MAXGZ MAXKG FAUN FAWE m m degree degree m m degree degree ---------------------------------------------------------------------- I01/SD01 16.726 7.407 0.1 75.4 1.72 16.495 - 10.8 I01/SD02 17.129 9.334 3.9 69.1 1.24 14.925 - 9.7 I01/SD03 15.569 0.712 2.4 76.5 1.95 16.291 37.9 19.6 I01/SD04 15.725 1.210 5.1 73.6 1.60 15.533 36.1 19.0 I01/SD05 15.606 0.490 3.8 74.6 1.66 15.736 38.5 19.3 I01/SD06 15.605 0.257 3.8 74.2 1.61 15.671 40.1 19.0 I01/SD07 15.607 0.025 3.8 73.6 1.54 15.558 41.9 18.1 I01/SD08 15.661 -0.484 3.9 72.7 1.48 15.441 43.8 17.8 I01/SD09 16.057 -2.964 2.8 72.5 1.63 15.941 45.9 15.2 I01/BB01 16.650 -4.496 15.0 64.4 0.86 13.483 41.9 18.2 I01/BB02 18.507 -6.851 0.3 79.7 1.49 16.426 42.1 14.4 I01/AD01 17.158 9.465 3.2 71.2 1.47 15.675 - 9.9 I01/AD02 15.783 1.536 4.7 74.8 2.05 16.273 38.9 19.4 I01/AD03 15.956 1.840 7.6 72.4 1.80 15.695 37.4 19.0 I01/AD04 15.894 0.611 6.7 73.0 1.86 15.837 39.5 19.2 I01/AD05 15.901 -0.243 6.8 72.7 1.83 15.790 40.6 18.9 I01/AD06 15.907 -1.103 6.9 72.4 1.80 15.740 41.8 18.0 I01/AD07 15.940 -2.068 6.7 71.9 1.74 15.679 43.2 17.9 I01/AD08 16.159 -3.585 3.6 72.7 1.76 16.066 45.4 15.3 ---------------------------------------------------------------------- STABILITY CRITERIA---------------------------------------------------------------------- CASE RCR SIDE REQ ATTV UNIT STAT ---------------------------------------------------------------------- I01/SD01 PROGR.M SB 0.0000 3.6047 m OK I01/SD01 MAXHEEL.M SB 30.0000 0.0622 deg OK I01/SD01 RANGE.M SB 20.0000 75.4464 deg OK I01/SD01 MAXGZ.M SB 0.1000 1.4167 m OK I01/SD01 MINAREA.M SB 0.0175 0.2531 mrad OK I01/SD02 PROGR.M SB 0.0000 2.2401 m OK I01/SD02 MAXHEEL.M SB 30.0000 3.9033 deg OK I01/SD02 RANGE.M SB 20.0000 69.0815 deg OK I01/SD02 MAXGZ.M SB 0.1000 1.1139 m OK I01/SD02 MINAREA.M SB 0.0175 0.2182 mrad OK I01/SD03 PROGR.M SB 0.0000 6.2186 m OK I01/SD03 MAXHEEL.M SB 30.0000 2.3821 deg OK I01/SD03 RANGE.M SB 20.0000 35.5630 deg OK I01/SD03 MAXGZ.M SB 0.1000 1.6231 m OK I01/SD03 MINAREA.M SB 0.0175 0.2844 mrad OK I01/SD04 PROGR.M SB 0.0000 5.1755 m OK I01/SD04 MAXHEEL.M SB 30.0000 5.1316 deg OK I01/SD04 RANGE.M SB 20.0000 30.9867 deg OK I01/SD04 MAXGZ.M SB 0.1000 1.4238 m OK I01/SD04 MINAREA.M SB 0.0175 0.2729 mrad OK---------------------------------------------------------------------- I01/SD05 MAXGZ.M SB 0.1000 1.4675 m OK I01/SD05 MINAREA.M SB 0.0175 0.2707 mrad OK I01/SD06 PROGR.M SB 0.0000 5.7500 m OK I01/SD06 MAXHEEL.M SB 30.0000 3.7807 deg OK I01/SD06 RANGE.M SB 20.0000 36.3582 deg OK I01/SD06 MAXGZ.M SB 0.1000 1.4409 m OK I01/SD06 MINAREA.M SB 0.0175 0.2666 mrad OK I01/SD07 PROGR.M SB 0.0000 5.8232 m OK I01/SD07 MAXHEEL.M SB 30.0000 3.7786 deg OK I01/SD07 RANGE.M SB 20.0000 38.1306 deg OK I01/SD07 MAXGZ.M SB 0.1000 1.3902 m OK I01/SD07 MINAREA.M SB 0.0175 0.2578 mrad OK I01/SD08 PROGR.M SB 0.0000 5.6213 m OK I01/SD08 MAXHEEL.M SB 30.0000 3.9415 deg OK I01/SD08 RANGE.M SB 20.0000 39.9153 deg OK I01/SD08 MAXGZ.M SB 0.1000 1.3400 m OK I01/SD08 MINAREA.M SB 0.0175 0.2522 mrad OK I01/SD09 PROGR.M SB 0.0000 4.7735 m OK I01/SD09 MAXHEEL.M SB 30.0000 2.7706 deg OK I01/SD09 RANGE.M SB 20.0000 43.1754 deg OK I01/SD09 MAXGZ.M SB 0.1000 1.4878 m OK I01/SD09 MINAREA.M SB 0.0175 0.2809 mrad OK I01/BB01 PROGR.M SB 0.0000 1.1593 m OK I01/BB01 MAXHEEL.M SB 30.0000 14.9772 deg OK I01/BB01 RANGE.M SB 20.0000 26.9608 deg OK I01/BB01 MAXGZ.M SB 0.1000 0.8032 m OK I01/BB01 MINAREA.M SB 0.0175 0.1846 mrad OK I01/BB02 PROGR.M SB 0.0000 1.1797 m OK I01/BB02 MAXHEEL.M SB 30.0000 0.3366 deg OK I01/BB02 RANGE.M SB 20.0000 41.7704 deg OK I01/BB02 MAXGZ.M SB 0.1000 1.3516 m OK I01/BB02 MINAREA.M SB 0.0175 0.2708 mrad OK I01/AD01 PROGR.M SB 0.0000 2.4491 m OK I01/AD01 MAXHEEL.M SB 30.0000 3.1838 deg OK I01/AD01 RANGE.M SB 20.0000 71.1723 deg OK I01/AD01 MAXGZ.M SB 0.1000 1.3319 m OK I01/AD01 MINAREA.M SB 0.0175 0.2651 mrad OK I01/AD02 PROGR.M SB 0.0000 5.0713 m OK I01/AD02 MAXHEEL.M SB 30.0000 4.7022 deg OK I01/AD02 RANGE.M SB 20.0000 34.1705 deg OK I01/AD02 MAXGZ.M SB 0.1000 1.7514 m OK I01/AD02 MINAREA.M SB 0.0175 0.3265 mrad OK I01/AD03 PROGR.M SB 0.0000 4.0023 m OK I01/AD03 MAXHEEL.M SB 30.0000 7.5870 deg OK I01/AD03 RANGE.M SB 20.0000 29.8682 deg OK I01/AD03 MAXGZ.M SB 0.1000 1.5989 m OK I01/AD03 MINAREA.M SB 0.0175 0.3230 mrad OK I01/AD04 PROGR.M SB 0.0000 4.2642 m OK I01/AD04 MAXHEEL.M SB 30.0000 6.7359 deg OK I01/AD04 RANGE.M SB 20.0000 32.7438 deg OK I01/AD04 MAXGZ.M SB 0.1000 1.6402 m OK I01/AD04 MINAREA.M SB 0.0175 0.3240 mrad OK I01/AD05 PROGR.M SB 0.0000 4.3450 m OK I01/AD05 MAXHEEL.M SB 30.0000 6.8126 deg OK I01/AD05 RANGE.M SB 20.0000 33.8385 deg OK---------------------------------------------------------------------- I01/AD06 MAXHEEL.M SB 30.0000 6.8793 deg OK I01/AD06 RANGE.M SB 20.0000 34.9723 deg OK I01/AD06 MAXGZ.M SB 0.1000 1.6020 m OK I01/AD06 MINAREA.M SB 0.0175 0.3187 mrad OK I01/AD07 PROGR.M SB 0.0000 4.0134 m OK I01/AD07 MAXHEEL.M SB 30.0000 6.6923 deg OK I01/AD07 RANGE.M SB 20.0000 36.4987 deg OK I01/AD07 MAXGZ.M SB 0.1000 1.5638 m OK I01/AD07 MINAREA.M SB 0.0175 0.3110 mrad OK I01/AD08 PROGR.M SB 0.0000 4.1796 m OK I01/AD08 MAXHEEL.M SB 30.0000 3.5919 deg OK I01/AD08 RANGE.M SB 20.0000 41.7904 deg OK I01/AD08 MAXGZ.M SB 0.1000 1.5932 m OK I01/AD08 MINAREA.M SB 0.0175 0.3031 mrad OK ----------------------------------------------------------------------INITIAL CONDITION : I02RESULTS---------------------------------------------------------------------- CASE T TR HEEL RANGE MAXGZ MAXKG FAUN FAWE m m degree degree m m degree degree ---------------------------------------------------------------------- I02/SD01 17.069 8.911 0.1 75.6 1.67 16.334 - 8.4 I02/SD02 17.349 10.232 3.0 71.1 1.28 14.849 - 8.6 I02/SD03 15.430 0.102 0.9 79.1 2.16 16.484 38.9 20.1 I02/SD04 15.585 0.607 3.5 76.5 1.82 15.681 37.1 19.4 I02/SD05 15.604 0.404 3.4 76.6 1.79 15.699 38.6 19.4 I02/SD06 15.604 0.186 3.4 76.6 1.74 15.630 40.2 18.9 I02/SD07 15.607 -0.035 3.4 76.6 1.67 15.511 42.0 18.0 I02/SD08 15.663 -0.539 3.6 75.9 1.60 15.397 44.0 17.7 I02/SD09 16.062 -3.053 2.5 75.4 1.75 15.902 46.1 15.1 I02/BB01 16.694 -4.518 14.2 65.8 1.00 13.460 42.0 18.3 I02/BB02 18.508 -6.933 0.2 79.8 1.61 16.424 42.2 14.1 I02/AD01 16.809 7.369 2.1 76.6 1.80 16.106 - 12.4 I02/AD02 15.697 1.073 3.6 76.4 2.25 16.375 39.8 20.0 I02/AD03 15.884 1.387 6.5 73.5 2.01 15.784 38.4 19.4 I02/AD04 15.907 0.532 6.3 73.7 1.99 15.784 39.6 19.3 I02/AD05 15.915 -0.317 6.4 73.6 1.96 15.735 40.8 18.8 I02/AD06 15.922 -1.174 6.4 73.6 1.92 15.684 42.0 18.0 I02/AD07 15.953 -2.136 6.3 73.7 1.87 15.620 43.3 17.8 I02/AD08 16.165 -3.671 3.3 75.5 1.88 16.025 45.5 15.2 ---------------------------------------------------------------------- STABILITY CRITERIA---------------------------------------------------------------------- CASE RCR SIDE REQ ATTV UNIT STAT ---------------------------------------------------------------------- I02/SD01 PROGR.M SB 0.0000 2.7764 m OK I02/SD01 MAXHEEL.M SB 30.0000 0.0632 deg OK I02/SD01 RANGE.M SB 20.0000 75.5976 deg OK I02/SD01 MAXGZ.M SB 0.1000 1.4088 m OK I02/SD01 MINAREA.M SB 0.0175 0.2592 mrad OK I02/SD02 PROGR.M SB 0.0000 2.1374 m OK I02/SD02 MAXHEEL.M SB 30.0000 3.0243 deg OK I02/SD02 RANGE.M SB 20.0000 71.0840 deg OK I02/SD02 MAXGZ.M SB 0.1000 1.1374 m OK I02/SD02 MINAREA.M SB 0.0175 0.2213 mrad OK I02/SD03 PROGR.M SB 0.0000 6.9188 m OK I02/SD03 MAXHEEL.M SB 30.0000 0.8790 deg OK I02/SD03 RANGE.M SB 20.0000 38.0389 deg OK I02/SD03 MAXGZ.M SB 0.1000 1.6909 m OK I02/SD03 MINAREA.M SB 0.0175 0.2837 mrad OK I02/SD04 PROGR.M SB 0.0000 5.9294 m OK I02/SD04 MAXHEEL.M SB 30.0000 3.4957 deg OK I02/SD04 RANGE.M SB 20.0000 33.5669 deg OK I02/SD04 MAXGZ.M SB 0.1000 1.5385 m OK I02/SD04 MINAREA.M SB 0.0175 0.2786 mrad OK I02/SD05 PROGR.M SB 0.0000 5.8372 m OK I02/SD05 MAXHEEL.M SB 30.0000 3.4061 deg OK I02/SD05 RANGE.M SB 20.0000 35.2194 deg OK---------------------------------------------------------------------- CASE RCR SIDE REQ ATTV UNIT STAT ---------------------------------------------------------------------- I02/SD06 MAXHEEL.M SB 30.0000 3.4074 deg OK I02/SD06 RANGE.M SB 20.0000 36.8415 deg OK I02/SD06 MAXGZ.M SB 0.1000 1.5195 m OK I02/SD06 MINAREA.M SB 0.0175 0.2792 mrad OK I02/SD07 PROGR.M SB 0.0000 5.9505 m OK I02/SD07 MAXHEEL.M SB 30.0000 3.4133 deg OK I02/SD07 RANGE.M SB 20.0000 38.6122 deg OK I02/SD07 MAXGZ.M SB 0.1000 1.4683 m OK I02/SD07 MINAREA.M SB 0.0175 0.2704 mrad OK I02/SD08 PROGR.M SB 0.0000 5.7355 m OK I02/SD08 MAXHEEL.M SB 30.0000 3.5863 deg OK I02/SD08 RANGE.M SB 20.0000 40.3902 deg OK I02/SD08 MAXGZ.M SB 0.1000 1.4181 m OK I02/SD08 MINAREA.M SB 0.0175 0.2651 mrad OK I02/SD09 PROGR.M SB 0.0000 4.8327 m OK I02/SD09 MAXHEEL.M SB 30.0000 2.5055 deg OK I02/SD09 RANGE.M SB 20.0000 43.5737 deg OK I02/SD09 MAXGZ.M SB 0.1000 1.5638 m OK I02/SD09 MINAREA.M SB 0.0175 0.2943 mrad OK I02/BB01 PROGR.M SB 0.0000 1.4314 m OK I02/BB01 MAXHEEL.M SB 30.0000 14.2365 deg OK I02/BB01 RANGE.M SB 20.0000 27.8222 deg OK I02/BB01 MAXGZ.M SB 0.1000 0.9087 m OK I02/BB01 MINAREA.M SB 0.0175 0.2058 mrad OK I02/BB02 PROGR.M SB 0.0000 1.1376 m OK I02/BB02 MAXHEEL.M SB 30.0000 0.2173 deg OK I02/BB02 RANGE.M SB 20.0000 42.0328 deg OK I02/BB02 MAXGZ.M SB 0.1000 1.4289 m OK I02/BB02 MINAREA.M SB 0.0175 0.2855 mrad OK I02/AD01 PROGR.M SB 0.0000 3.6164 m OK I02/AD01 MAXHEEL.M SB 30.0000 2.1134 deg OK I02/AD01 RANGE.M SB 20.0000 76.5889 deg OK I02/AD01 MAXGZ.M SB 0.1000 1.5589 m OK I02/AD01 MINAREA.M SB 0.0175 0.2981 mrad OK I02/AD02 PROGR.M SB 0.0000 5.6340 m OK I02/AD02 MAXHEEL.M SB 30.0000 3.5865 deg OK I02/AD02 RANGE.M SB 20.0000 36.2347 deg OK I02/AD02 MAXGZ.M SB 0.1000 1.8507 m OK I02/AD02 MINAREA.M SB 0.0175 0.3327 mrad OK I02/AD03 PROGR.M SB 0.0000 4.5032 m OK I02/AD03 MAXHEEL.M SB 30.0000 6.4999 deg OK I02/AD03 RANGE.M SB 20.0000 31.8955 deg OK I02/AD03 MAXGZ.M SB 0.1000 1.7291 m OK I02/AD03 MINAREA.M SB 0.0175 0.3368 mrad OK I02/AD04 PROGR.M SB 0.0000 4.4394 m OK I02/AD04 MAXHEEL.M SB 30.0000 6.3000 deg OK I02/AD04 RANGE.M SB 20.0000 33.2899 deg OK I02/AD04 MAXGZ.M SB 0.1000 1.7232 m OK I02/AD04 MINAREA.M SB 0.0175 0.3370 mrad OK I02/AD05 PROGR.M SB 0.0000 4.4718 m OK I02/AD05 MAXHEEL.M SB 30.0000 6.3724 deg OK I02/AD05 RANGE.M SB 20.0000 34.3911 deg OK I02/AD05 MAXGZ.M SB 0.1000 1.7048 m OK I02/AD05 MINAREA.M SB 0.0175 0.3345 mrad OK I02/AD06 PROGR.M SB 0.0000 4.1599 m OK。
甘肃省嘉峪关市市级名校2024年中考英语最后冲刺浓缩精华卷含答案
甘肃省嘉峪关市市级名校2024年中考英语最后冲刺浓缩精华卷含答案注意事项：1．答卷前，考生务必将自己的姓名、准考证号、考场号和座位号填写在试题卷和答题卡上。
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Ⅰ. 单项选择1、– I ______ a lot of time playing computer games every day.–That’s bad for your eyes.A．spendB．takeC．payD．cost2、--How can I get some ________ about the Boao Forum for Asia(博鳌亚洲论坛)?--Why not search the Internet?A．information B．experience C．practice3、Here is a message for Jake from Peter. What does Peter want?Hi JakeSorry, I can't make it to thecinema this weekend as a friendis coming to stay. Can we go nextSaturday? Call me and I'll bookthe ticketsPeterA．He wants his friend to get the tickets.B．He wants Jake to come to meet his friendC．He wants to go to the cinema one week later.D．He wants to bring another friend along to the cinema.4、Think of others more than yourself. A person______cares for others is popular everywhere.A．who B．which C．whom D．whose5、There will be an important test tomorrow morning. You be late.A．may not B．needn’tC．mustn't D．couldn’t6、students in the classroom twenty. The others are in the library.A．The number of; isB．A number of; areC．The number of; are7、Mr. Green is very strict with his children, and he ______ allows them to watch TV on school days.A．always B．usually C．sometimes D．never8、This is one of the most interesting cities I have ever visited.A．who B．that C．which D．where9、I the school bus this morning. Luckily, Tony's father gave me a ride.A．took B．caught C．saw D．missed10、The girl ______ long black hair is Lily. She is pretty.A．with B．at C．inⅡ. 完形填空11、Many people believe that 3G mobile phones will be used in almost all everyday activities. It____that we won’t go shopping because most things will be available(可得到的) on the Internet. There will be no more____ because all texts will be available in electronic libraries. The Internet will be used to plan holidays, rent films and_____ food. Most telephone calls will be ____ by 3G mobile phones as well.Some people are ____ about the new development. Others, ho wever, don’t think that 3G mobile phones will replace the old 1 of shopping and communicating.Let’s look at books, for example. Some people believe that one day we won’t read books made of paper. ____, we’ll read books by using 3G mobile phones. These mobile phones will store the contents（内容）of many different books at the same time. We won’t need to turn lots of pages and paper will be ____. Is the Internet shopping as fun as traditional shopping? Many people say it’s not. It’s 2 to go into shops and look at things. It’s also 3 that most people will want to read books on their 3G mobile phones. Maybe 3G mobile phones won’t change these two habits.1．A．is thinking B．has thought C．is thought D．to think2．A．dictionaries B．people C．letter D．books3．A．order B．heat C．eat D．cook4．A．built B．made C．bought D．used5．A．afraid B．interested C．worried D．excited6．A．ways B．time C．places D．houses7．A．Besides B．However C．Instead D．So8．A．sold B．saved C．wasted D．collected9．A．boring B．interested C．terrible D．fun10．A．important B．comfortable C．impossible D．normalⅢ. 语法填空12、A：Li Ping, have you seen the science fiction film “The Wandering Earth”（流浪地球）recently?B：Yes, I have. I saw it during the Spring Festival.A：It was reported that the film was great. What does it tell about?B：It tells a story about 1．humans, threatened（威胁）by a dying and swelling sun, build very large engines to push the planet out of the solar system, starting a centuries-long journey in search of a new sun.A：Y ou mean our earth is in great danger and we should try to save it, right?B：Yes, quite right. As we all know, with the 2．of modern agriculture and industry, more and more pollution is produced. We all should have a strong sense of 3．protection. Or we humans won’t find places to live in.A：Sure. But in your opinion, what 4．of pollution are there?B：There are many. The most important ones are water, air and land pollution.A：Can you tell me what caused water pollution and how we should solve these problems？B：As we can see, people are 5．litter into the river and factories are putting waste into the river, so water is polluted.I think we should require our government to close down those factories and call on people to clean up the river and 6．every kind of pollution.A：Do you have any ideas for solving the air pollution?B：Well, to cut down air pollution, we should take the bus or subway instead of driving. Of course, riding a bike is also a good choice. It’s good for health and it 7．costs anything!A：What else can we do to help save the earth?B：Nothing is waste if we have a 8．mind. We can put those things we don’t need to good use. We can rethink, reuse and recycle them!A：Any good examples to share with me?B：We can learn from Amy Hays, a most unusual woman in the UK. She lives in a house that she built 9．out of rubbish. Jessica Wong from Hong Kong made handbags by using old clothes, especially old jeans. Both of them are good at recycling and they are a(n) 10．to us all.A：They’re great! If only we join together to fight against pollution and learn to recycle, we will make a difference and lead to a better earthⅣ. 阅读理解A13、Having a teenager at home can be a real headache. Many teenagers smoke, wear strange hairstyle and even fight. Basically (基本上) , they are often taking risks.Earlier studies have shown that teenagers are more likely to makeirrationaldecisions than people from any other age group, including children and adults. Is it that teenagers are too young to tell right from wrong? Not really. So what’s the reason?According to Laurence, a teacher from a US university, the reason is that teenagers care a lot about how their peers (同龄人) view them—that is “peer influence”.As children enter their teenage years, they spend more time with their friends and classmates, and also they care more about what they think of them. This makes teenagers make decisions without thinking about the costs.In a test, a group of teenagers were asked to play a video driving game. When they played with their friends watching around them, they took more chances and drove more carelessly because that would increase their possibility of winning. But when they played alone, they drove more safely.Why do peers have such a big influence on teens’ behavior?As Laurence sees it, a teenager’s brain is like a car with a good accelerator (油门) but a weak brake (刹车). The “accelerator” is fully developed by teenage years while the “brake” is still not fully develop ed. When teenagers are watched by their peers, they usually push hard on the accelerator. With their weak brake, it is likely that they are going to end up in an accident. But the good news is that a violent (暴力的) teenager doesn’t necessarily become a viol ent adult. About two-thirds to three-quarters of violent youth grow out of it. “They get more self-controlled.”1．What does the underlined word “irrational” in the 2nd paragraph mean?A．Quick. B．Crazy.C．Careful. D．Correct.2．Why are teenagers often taking risksaccording to the text?A．They like difficult tasks.B．They don’t care about costs.C．They are too young to tell right from wrong.D．They care a lot about what their peers think of them.3．How does Laurence explain the influence of peer influence on t eens’ behavior?A．By using a metaphor (比喻).B．By giving examples.C．By presenting research findings.D．By performing an in-depth analysis (分析).4．According to the text, with peers around them, teenagers tend to (倾向于) ______. A．use more self-controlB．become more careful about what they doC．perform better than when they are aloneD．focus more on short-term benefits（利益）5．What can we learn from the text?A．Teenagers are easy to have traffic accidents.B．Peer influence is bad on teenagers.C．Most of the teenagers become less violent when they grow up.D．Teenagers shouldn’t spend all the free time with their friends.B14、1．What is the most probably reason the sweater is the girl’s favorite?A．The girl’s grandma made it.B．The girl got it at the age of three.C．The yarn is the color of bluebells.D．The cat uses it for the kittens.2．The word “kitten”in this poem most probably means ____.A．a kind of food B．a baby catC．a kind of drink D．a bed for a baby3．Why has the girl given the sweater away?A．The girl wants her grandma to make a new sweater.B．The girl will no longer like the sweater.C．The girl likes her new jacket better than the sweater.D．The girl is too big for the sweaterC15、In 1996, Joe Richman had been working as a radio reporter on US national radio for several years and was looking for a new idea for a radio programme. He met a teenager called John Cutler. John had Tourette's Syndrome—a brain illness. This meant that Josh did not always have control of his movements and speech.John was just beginning high school when he agreed to work with Joy on a story about his illness. Joe wanted John to keep a radio diary of his daily life. He gave John a tape recorder and asked him to record what happened to him every day. John and Joe agreed that an important part of his story would be talking to his school friends about Tourette's Syndrome. John was embarrassed(尴尬) to record at school—his friends might laugh.One day, John took the tape recorder to school, but he kept it inside his bag all day. He was too afraid to bring it out. After months of feeling afraid, he took the tape recorder out on lunch time. What happened next was a total surprise. John tells it best:“Everyone jumped at the chance. Everyone started asking me questions about that, I had never really talking about it to anyone—except my mom and dad. ”That was an important day in John's life because he learned to be more open about his condition. Now John says he wants to do radio diaries until he's 80 years old. He learnt a lot about himself by doing the radio diary. It made a great radio programme, too. Being a reporter is like having a passport. You can ask questions, be curious and explore new worlds. Radio is the perfect way to be on the radio and everyone likes to listen!1．Who was Joe Richman?A．John's teacher. B．A radio reporter.C．John's doctor. D．One of John's classmates.2．What did Joe want John to do?A．To keep a radio diary of his daily life. B．To talk to his school friends.C．To take the tape recorder to school. D．To control his movements and speech.3．How did John's classmates feel when he recorded for the first time?A．Afraid. B．Shy.C．Excited. D．Strange.4．What did John learn by doing the radio diary?A．To be more open. B．A lot about himself.C．To make more friends. D．Much about radio programmeD16、As a little boy, there was nothing I liked better than Sunday afternoons at my grandfather's farm in western Pennsylvania. Surrounded by miles of winding stonewalls, the house and farm provided endless hours of fun for a city kid like me.I can still remember one afternoon when I was eight years old. Since my first visit to the farm, I'd wanted more than anything to be allowed to climb the stonewalls surrounding the farm. My parents would never agree. The walls were old; some stones were missing, others loose and falling into pieces. Still, my wish to climb across those walls grew so strong that finally, one spring afternoon, I summoned (鼓起) all my courage and entered the living room, where the adults had gathered after Sunday dinner."I, uh, I want to climb the stonewalls," I said hesitantly. Everyone looked up. "Can I climb the stone walls?" Immediately a chorus went up from the women in the room. "Heavens, no!" they cried, "You'll hurt yourself!" I wasn't too disappointed; the response was just as I'd expected. But before I could leave the room, I was stopped by my grandfather's loud voice. "Now hold on just a minute," I heard him say, "Let the boy climb the stone walls. He has to learn to do things for himself.'"Go," he said to me, "and come and see me when you get back. ' For the next two and a half hours I climbed those old walls and had the time of my life. Later I met with my grandfather to tell him about my adventure. I'll never forget what he said. "Fred," he said, smiling widely, "you made this day a special day just by being yourself. Always remember: there's only one person in this whole world like you, and I like you as you are."Many years have passed since then, and today I host the television program Mister Rogers'Neighborhood, seen by millions of children throughout America. There have been changes over the years, but one thing remains the same: my message to children at the end of almost every visit."There's only one person in this whole world like you," the kids can count on hearing my say, "and people can like you as you are."1．Which of the statements is TRUE according to the passage?A．As a city kid, Fred was unwilling to go to his grandfather's farm.B．Fred helped his grandfather build the stonewalls to protect the farm.C．Fred was sure that his parents would agree with his climbing the stonewalls.D．Fred' grandfather encouraged him to do things for himself.2．Why wasn't Fred allowed to climb the stonewalls at first?A．Because he was too young to climb the stonewalls on his own.B．Because his parents were very strict with him.C．Because his family thought it was dangerous to climb the stonewalls.D．Because his grandpa didn't like him going out.3．What can we infer from the passage?A．The TV program Mister Rogers' Neighborhood is popular with children in America.B．Fred's grandpa wanted Fred to be a host of the TV program.C．There's only one person in this whole world that likes Fred as he is.D．When Fred was young, he didn't take his parents' opinions seriously.E17、When you look up into the night sky, you can see the moon. But did you know that you’re only seeing one side of the moon? The other side, also known as the far side of the moon, has long been a secret to us__But now, we have a chance to see what’s on the far side.On Jan 3, China’s Chang’e 4 probe (探测器) landed on the far side of the moon. It is the first probe in history to land there.Chang’e 4 has already done a lot of things on the moon. One of its jobs is to grow plants. Chang’e 4 took six living things to the moon, such as cotton , potatoes and fruit flies. The cotton has already started growing. This is the first time humans have grown plants on the moon.Chang’e 4 also took a lot of pictures of the moon’s far side. With these pictures, scientists will learn more about the moon.1．What does the underlined sentence in Paragraph 1 mean?A．There is a secret place on the other side.B．We are not interested in the far side.C．We don’t know much about t he other side.D．The other side has already been known to humans.2．What do we know about the Chang’e 4 probe?A．It landed on the far side of the moon.B．It left Earth on Jan 3.C．It is the first probe made by China.D．It has found out all of the moon’s se crets.3．What has Chang’e 4 done so far?a. It has grown some plants on the moon.b. It has become the first probe to land on the moon.c. It has built a probe on the moon.d. It has taken photos of the far side of the moon.A．a b B．a d C．c d D．b cF18、Have you heard of Aqua exercises? They are exercises done in water. They are also called water exercises,. It is believed that aqua exercises can help people burn more fat than exercises on dry land .There are two main types of aqua exercises.First, aqua jogging. We must wear a special belt which can protect us and help us floating as we run in the water. Although we are in water, we should run very hard as if we were jogging on dry land.Aqua jogging can protect our joints(关节)and at the same time make our muscles (肌肉) work hard.The famous actress, Jennifer Aniston, is reported to aqua jog for fifteen minutes every day. Now there are many aqua jogging clubs and classes all over the UK.Second, aqua Tai Chi .Tai Chi on land is an ancient Chinese exercise designed to improve balance, reduce stress and help people to relax. Aqua Tai Chi can do this better. Peter Chou , who has established a Tai Chi school , says, “Tai Chi is about fluidity(流动性) and relaxation. For thousands of years, experts have been asking people to imagine being in water when they are practicing Tai Chi, so why not actually put them in water? When their hands and feet move slowly through water, the people can experience what fluidity feels like both mentally and physically, and understandt his exercise better. The water can also make them slower and more relaxed.”People will need an understanding of Tai Chi and should be able to perform some of the simple movements on land before trying aqua Tai Chi. It is not suitable for beginners becaus e they need to see how the teachers’ hands and feet are moving, which is not easily done underwater.根据材料内容选择最佳答案，并将其标号填入题前括号内。
颅内压增高、颅内高压症英文课件
Ⅰ、 Anatomy--- The cranial cavity
The cranial cavity: closed container / poorly distensible
Ⅰ、 Anatomy--- The cranial cavity
brain tissue (80%、1400ml)
cerebral blood
(3) Brain tissue compromise little room Brain tissue compromise little room to regulate ICP
2. Cranial cavity of the adult can’t regulate ICP Cranial cavity of the adult is fixed
NEUROSURGERY
Increased Intracranial Pressure
(Increased ICP, Raised ICP, Intracranial Hypertension)
Increased Intracranial Pressure
Ⅰ、 Anatomy Ⅱ、 Regulation of ICP Ⅲ、 Increased Intracranial Pressure Ⅳ、 Pathogenesis Ⅴ、 Clinical Symptoms and Signs Ⅵ、 Diagnosis of Increased Intracranial Pressure Ⅶ、 Effects of Increased Intracranial Pressure Ⅷ、 Treatment Ⅸ、 Brain herniation
Superior sagittal sinus
雷蛇Kraken 7.1 Chroma USB游戏耳机 说明书
Get the complete 7.1 surround sound gaming experience with the Razer Kraken 7.1 Chroma USB gaming headset. This headset adopts the comfortable form factor of the Razer Kraken Pro, tested by numerous professional gamers to determine the optimal ergonomics for extended gaming sessions.The full potential of the Razer Kraken 7.1 Chroma is unleashed by its advanced 7.1 virtual surround sound engine. Driven by Razer’s powerful Synapse unified configuration software, this highly customizable engine modulates sound to simulate a 360⁰ surround sound experience, allowing you to precisely pinpoint directional audio to know exactly where your enemies are. The headset’s 40mm neodymium magnet drivers power a sound signature that features deep bass, warm mids, and crystal-clear highs for total gaming immersion.Featuring a retractable microphone in the left ear cup that stays hidden when not in use, the Razer Kraken 7.1 Chroma maintains a sleek form factor while protecting the microphone during transportation and storage. This flexible, omnidirectional digital microphone comes equipped with an optimized algorithm, promising pristine voice quality unachievable through traditional analog microphones. Boasting an impressive signal-to-noise ratio and an extended wideband frequency response, the digital microphone ensures a clear, natural sounding voice reproduction with minimal noise. Now with Chroma customizable lighting on the ear cups this headset offers both personalized surround sound and color.TABLE OF CONTENTS1. PACKAGE CONTENTS / SYSTEM REQUIREMENTS (2)2. REGISTRATION / TECHNICAL SUPPORT (2)3. TECHNICAL SPECIFICATIONS (3)4. DEVICE LAYOUT (4)5. USING YOUR RAZER KRAKEN 7.1 CHROMA (5)6. INSTALLING YOUR RAZER KRAKEN 7.1 CHROMA (8)7. CONFIGURING YOUR RAZER KRAKEN 7.1 CHROMA (9)8. SAFETY AND MAINTENANCE (19)9. LEGALESE (20)1. PACKAGE CONTENTS / SYSTEM REQUIREMENTSPACKAGE CONTENTS∙Razer Kraken 7.1 Chroma Surround Sound USB Gaming Headset∙Important Product Information GuideSYSTEM REQUIREMENTS∙PC/Mac with USB port∙Windows® 8 / Windows® 7 / Windows Vista® / Mac OS X (10.7-10.9)∙Internet connection (for driver installation)∙At least 100MB of free hard disk space2. REGISTRATION / TECHNICAL SUPPORTREGISTRATIONSign up now for a Razer Synapse account to get real-time information on your product’s warranty status. To learn more about Razer Synapse and all its features, visit /synapse.If you are already a Razer Synapse user, register your product by clicking on your email address at the Razer Synapse application and select Warranty Status from the dropdown list.To register your product online, visit /registration. Please note that you will not be able to view your warranty status if you register via the website. TECHNICAL SUPPORTWhat you’ll get:• 1 year limited manufacturer’s warranty.• Free online technical support at .3. TECHNICAL SPECIFICATIONS HEADPHONES∙Drivers: 40mm neodymium magnets∙Frequency Response: 20Hz - 20kHz∙Impedance: 32 Ω∙Sensitivity @ 1kHz: 112dB∙Output Power: 30mW∙Connector: Gold plated USB∙Cable Length: 2m / 6 ft braided USB cable ∙Approximate Weight: 340g MICROPHONE∙Frequency response: 100Hz –12kHz∙Sensitivity @ 1kHz: -40dB ± 4dB∙Signal-to-noise ratio: 63 dB∙Pick-up pattern: Unidirectional4. DEVICE LAYOUTA.Adjustable padded headbandB.Foldable ear cup designC.Circumaural, leatherette ear cushionsD.Multi-color LED logo lightingE.Retractable microphoneF.Microphone mute/unmute LEDG.Microphone mute/unmute buttonH.Gold plated USB connector5. USING YOUR RAZER KRAKEN 7.1 CHROMASETTING THE DEFAULT PLAYBACK DEVICEIf you are using the Razer Kraken 7.1 Chroma headset for the first time, you may be required to set up the device as your system’s default playb ack device.For Windows usersStep 1: Open your Sound settings from Control Panel > Hardware and Sound > Manage audio devices. You can also right-click your sound icon on the system tray and select Playback devices.Step 2: In the Playback tab, select Razer Kraken 7.1 Chroma from the list and click the Set Default button.For Mac usersStep 1: Open your Sound settings from System Preferences > Sound.Step 2: In the Output tab, select Razer Kraken 7.1 Chroma from the list.Step 3: In the Input tab, select Razer Kraken 7.1 Chroma from the list.USING THE RETRACTABLE MICROPHONEPress the button to mute/unmute the microphone. The microphone LED will light up when muted.EXTENDING YOUR HEADPHONE’S LIFESPANWe recommend stretching the headphones gently apart before placing them over your head to minimize headband stress. However, please avoid overstretching the headphones beyond its technical limits.Step 1: Connect your Razer device to the USB port of your computer.Step 2: Install Razer Synapse when prompted* or download the installer from /synapse.Step 3: Create your Razer ID or login to Synapse with your existing Razer ID.*Applicable for Windows 8 or later.Note:∙By default, the Razer Kraken 7.1 Chroma headset works out of the box as a (stereo) headset. To achieve7.1 surround sound, please install Synapse to set up virtual 7.1 surround sound.∙By default, the Razer Kraken 7.1 Chroma is set to spectrum cycling. To customize the lighting, pleaseinstall Razer Synapse.Disclaimer: The features listed here require you to log in to Razer Synapse. These features are also subject to change based on the current software version and your Operating System.Your Razer Kraken 7.1 Chroma headset is equipped with various software customizable features to unleash its full power.CALIBRATION TABA 3 step calibration wizard helps you set up your Razer Kraken 7.1 Chroma for virtual7.1 surround sound. If you are setting up your audio device for the first time, we recommend using this wizard for a step by step guide on the calibration process.Click START to launch the wizard.Step 1 of 3This step introduces the calibration process. Read the instructions carefully and ensure that you are in a quiet environment in order to hear the audio prompts properly.In this figure, the mannequin represents your position. The green arrows on each segment represent the target direction of the audio playback for that particular segment. Each orange line represents the last saved calibration settings configured on Razer Surround.Click Calibrate Now once you are ready to begin the calibration.Step 2 of 3Position yourself according to the point of reference indicated by the mannequin. The calibration process is divided into a series of segments. An audio prompt will be played for each segment.For the first segment, the sound should be perceived as coming from the direction marked by the green target arrow, roughly North-East of your point of reference. Your aim is to move the sound you hear such that you perceive it to be coming from this target direction. To achieve this,1.Listen carefully to the audio prompt2.The sound you hear will change direction as you move the mouse scroll wheel orthe up/down arrow keys.3.Continue changing the direction of the sound until you perceive it to come fromthe North-East position or as close to the green target arrow as possible. Note: The orange line indicates the relative change to the previously saved alignment setting. It does not affect your current calibration, as it serves merely as a change indicator.Click Next to continue to the next segment. Repeat the above, using the green target arrow for each segment as the target direction and continue until you complete the circle.Once all segments are calibrated, click Next to go to the next step.Step 3 of 3Click Experience Surround Sound to test your personalized sound environment. To tweak a particular section, simply click it to play its audio prompt and adjust the settings as desired. To reset the entire configuration, click Reset All. Otherwise, click Finish to confirm the settings.A confirmation screen will be displayed. Click OK to save and exit the wizard.AUDIO TABThe Audio Tab lets you control various options related to the audio you hear from your headset.Using the various sliders, you can:∙Adjust the overall volume of your headset∙Improve the bass output of your headset∙Normalize the loudness of audio played to avoid sudden and unpleasant increase in volume from effects such as explosions∙Enable voice clarity adjustments to improve the quality of incoming voice conversations. Voice Clarity adjustments includes both clarity (Presence Level) and volume (Volume Level) of incoming voice conversations.The Mic Tab lets you control various options related to how your headset handles voice input via its microphone.Using the various sliders and options you can:∙Adjust the microphone input volume. You can also choose to mute your microphone.∙Adjust volume normalization level for microphone input∙Adjust the microphone’s sensitivity so it cuts out background noises and only picks up your voice.∙Enhance the clarity of microphone input by reducing ambient noise. Use the slider to determine the level of noise reduction desired.The Mixer Tab allow you to adjust the volume for programs currently running on your system individually.Note: The Mixer Tab is not available for Mac OS systems.Customize the volume of audio for each program using the sliders.EQ TABThe EQ Tab lets you filter various audio frequencies, controlling the overall tone of your audio output.A list of preset equalizer settings can be selected from the drop down menu, giving you easy access to various commonly used audio tones. You can also manually adjust each audio frequency using the sliders.LIGHTING TABThe Lighting Tab is where you can customize the color of Razer logo lighting or select pre-loaded lighting effects. Changes made in this tab will be automatically saved to the current profile.Note that the color of the microphone LED cannot be changed.Lighting options include:∙Spectrum Cycling: Logo lighting will cycle between 16 million colors indefinitely.This is the default lighting effect.∙Breathing: making the logo lighting fade in and out of the selected color.8. SAFETY AND MAINTENANCESAFETY GUIDELINESIn order to achieve maximum safety while using your Razer Kraken 7.1 Chroma, we suggest that you adopt the following guidelines:1. Should you have trouble operating the device properly and troubleshooting does not work, unplug the device and contact the Razer hotline or go to for support. Do not attempt to service or fix the device yourself at any time.2. Do not take apart the device (doing so will void your warranty) and do not attempt to service it yourself or operate it under abnormal current loads.3. Keep your device away from liquid, humidity or moisture. Operate your device only within the specified temperature range of 0˚C (32˚F) to 40˚C (104˚F). Should you operate it in a temperature that is beyond this range, unplug and switch off the device in order to let the temperature stabilize within the optimal temperature range.4. The device isolates external ambient sounds even at low volumes, resulting in lowered awareness of your external surroundings. Please remove the device when engaging in any activities that requires active awareness of your surroundings.5. Listening to excessively loud volumes over extended periods of time can damage your hearing. Furthermore, legislation of certain countries permits a maximum sound level of 86db to affect your hearing for 8 hours a day. We therefore recommend that you reduce the volume to a comfortable level when listening for prolonged periods of time. Please, take good care of your hearing. MAINTENANCE AND USEThe Razer Kraken 7.1 Chroma requires minimum maintenance to keep it in optimum condition. Once a month we recommend you unplug the device and clean it using a soft cloth or cotton swab with a bit of warm water to prevent dirt buildup. Do not use soap or harsh cleaning agents.9. LEGALESECOPYRIGHT AND INTELLECTUAL PROPERTY INFORMATIONCopyright ©2014 Razer Inc. All Rights Reserved. Razer, the Razer Triple-Headed Snake logo, the Razer distressed word logo and other trademarks contained herein are trademarks or registered trademarks of Razer Inc. and/or its affiliated or associated companies, registered in the United States and/or other countries. Windows and the Windows logo are trademarks of the Microsoft group of companies. Mac OS, Mac and the Mac logo are trademarks or registered trademarks of Apple. All other trademarks are the property of their respective owners.Razer Inc. (“Razer”) may have copyright, trademarks, trade secrets, patents, patent applications, or other intellectual property rights (whether registered or unregistered) concerning the product in this guide. Furnishing of this guide does not give you a license to any such copyright, trademark, patent or other intellectual property right. The Razer Kraken 7.1 Chroma (the “Product”) may differ from pictures whether on packaging or otherwise. Razer assumes no responsibility for such differences or for any errors that may appear. Information contained herein is subject to change without notice.LIMITED PRODUCT WARRANTYFor the latest and current terms of the Limited Product Warranty, please visit /warranty.LIMITATION OF LIABILITYRazer shall in no event be liable for any lost profits, loss of information or data, special, incidental, indirect, punitive or consequential or incidental damages, arising in any way out of distribution of, sale of, resale of, use of, or inability to use the Product. In no event shall Razer’s liability exceed the retail purchase p rice of the Product.COSTS OF PROCUREMENTFor the avoidance of doubt, in no event will Razer be liable for any costs of procurement unless it has been advised of the possibility of such damages, and in no case shall Razer be liable for any costs of procurement liability exceeding the retail purchase price of the Product.20 | For gamers by gamers™GENERALThese terms shall be governed by and construed under the laws of the jurisdiction in which the Product was purchased. If any term herein is held to be invalid or unenforceable, then such term (in so far as it is invalid or unenforceable) shall be given no effect and deemed to be excluded without invalidating any of the remaining terms. Razer reserves the right to amend any term at any time without notice.21 | razer。
Wang et al
International Journal of the Physical Sciences Vol. 6(1), pp. 112-115, 4 January, 2011Available online at /IJPSISSN 1992 - 1950 ©2011 Academic JournalsFull Length Research PaperProtective effect of Morinda officinalis polysaccharides on bone degeneration in the aged ratsZong-Bao Wang1 Qing-You Lu2* Hai-Yan Lu1 Wei-Ming Liao3, Zhuo-Peng Wu1 Guang-ZhiKuang1 and Hua-Jie Feng11Science Research Base of Kaiping Central Hospital Postdoctoral Mobile Station of Sun Yat-sen University Kaiping529300 China.2Department of Traumatic Surgery Dongfang Hospital Affiliated to Tongji University Shanghai 200120 China.3Department of Orthopedic First Hospital Affiliated to Tongji University, Guangzhou 510700 China.Accepted 22 December, 2010In the current study, we examined the effect of Morinda officinalis polysaccharides (MOP) on bone quality in aged rats. Rats were given orally by daily gavage at 200 and 400 mg/kg/day for 3 months, respectively. The results showed that there was a significant decrease in serum alkaline phosphatase (ALP), Interleukin 6 (IL-6) content and a significant increase in density and strength of femur and lumbar vertebrae in aged rats 3 months after Morinda officinalis polysaccharides treatment. In conclusion, these data suggest that Morinda officinalis polysaccharides can protect the age-induced bone degeneration.Key words:Morinda officinalis polysaccharides, bone, osteoporosis, ALP.INTRODUCTIONOsteoporosis is characterized by low bone mass and enhanced fragility of the bones, making the bones susceptible to fractures from minor trauma, and it is often associated with aging. Until recently, the focus of age-related bone loss has been on postmenopausal women mainly because women start loosing bone earlier than men and the bone loss proceeds more rapidly in women than in men (Ferrari, 2008; International Osteoporosis Foundation, 2006). In addition to perfusion, systemic metabolism and the influence of medication, age plays an important role as a biological factor. Animal experiments in rats as well as clinical studies in humans show a delayed course of bone healing with increasing age (Meyer et al., 2001; Skak and Jensen, 1988). As reasons for this, a reduced number of osteogenic stem cells, their reduced proliferation and differentiation potential, and reduced systemic or local blood flow have been discussed (Battmann et al., 1997; Bloomfield et al., 2002;*Corresponding author E-mail: qingyou266@ Quarto, et al., 1995; Silbermann et al., 1987). Chinese traditional tonic herbal medicine BaJiTian (Morinda officinalis) has been extensively used in China for about two thousand years, for tonifying kidney, strengthening Yang-qi, relieving rheumatism and so on. It is used in Chinese medicine to nourish “Kidney-Yang” and strengthen the “Bone and Muscle” indicated for impotence, emission, enuresis, infertility, and to treat Cold and Dampness syndromes such as rheumatism, pain and fatigue (Zhang et al., 2002). Recently, studies on M. officinalis including chemical ingredients and relevant pharmacological properties have been performed. Iridoids isolated from M. officinalis have displayed obvious antinociceptive and anti-inflammatory effects (Choi et al., 2005), oligosaccharides have been shown to possess antidepressant effects and its anthraquinones have been isolated (Li et al., 2004). Polysaccharides have also been determined and recently were shown to have protective effect against bone loss (Wu et al., 2005). The objective of the present study was to evaluate the protective effect of M. officinalis polysaccharides on bone degeneration in the aged rats.MATERIALS AND METHODSMaterialsM. officinalis was obtained from Changsheng herb shop, China. The material (No. 2009-0004) was identified by Professor C.Y Wang, College of Pharmacy, Our University, China.Extraction of M. officinalis polysaccharidesM. officinalis samples were grind into fine powder and defatted with ethanol for 10 h and extracted three times with boiling water (100°C), each time for 2 h. The extracts were then filtered and concentrated under reduced pressure. The crude polysaccharide fraction, termed MOP, was obtained by ethanol precipitation at the final concentrations of 80% of ethanol.Animals and treatmentsThirty-two male Wistar rats were provided from our institute (Guangzhou city; China). They were kept in a temperature-controlled environment (20±1°C) with a 12:12-h light–dark cycle, and fed standard chow, for at least 1 week before any manipulation. Rats were kept and treated in accordance with the China regulations concerning animal care, in an approved environment. Animals were divided into four group: Normal control group (NC) (aged 3 months, body weight 137±11 g), Model control group (MC) (aged 18 months, body weight 327±18 g), two MOP-treated group (aged 18 months). Each group included eight animals. In the two MOP-treated groups, M. officinalis polysaccharides was given orally by daily gavage at 200 and 400 mg/kg/day for 3 months, respectively, with eight animals per dose. In normal control group (NC), rats was given orally with equal volume of saline for 3 months. In the model control group (MC), rats was given orally with equal volume of saline for 3 months.At the end of the experiment, all animals were anesthetized via an intraperitoneal injection of Ketamine/Xylazine (Ketamine: 100 mg/ml, 90 mg/kg; Xylazine: 20 mg/ml, 10 mg/kg), and then exsanguinated via cardiac puncture. Blood and tissue samples were removed for further analysis.Serum biochemical parameters assaySerum ALP was measured on an automatic analyzer (Ciba-Corning 550, USA) using a diagnostic reagent kit. Serum IL-6 was estimated using an ELISA kit according to the manufacturer's instructions.Femur and lumbar vertebrae density measurements by peripheral quantitative computed tomography (pQCT)Femur length was measured with digital calipers (Stoelting, Wood Dale, IL). Isolated femurs and lumbar vertebrae were measured for density using pQCT (Stratec XCT 960 M, Norland Medical Systems, Ft. Atkinson, WI). Femurs and lumbar vertebrae were assessed for cross-sectional and volumetric bone parameters using a multispecimen holder designed for the XCT 960 M. Analysis was carried out by a modification of the previously described method (Beamer et al., 1998). Voxel size was reduced to 0.07 mm for the analysis. The bone scans were analyzed with two different outer threshold settings to separate bone from soft tissue. An outer (and inner) threshold of 630 mg/cm3 was used to determine bone areas and surfaces. These thresholds were selected to yield area values consistent with histomorphometrically derived values. To determine mineral content, a second analysis was carried out with an outerWang et al. 113threshold setting of 230 mg/cm3. This lower threshold was selected so that mineral from most partial voxels would be included in the analysis. Density values were calculated from the analyzed areas and associated mineral contents. Total volumetric density (vBMD) values were calculated by dividing the total mineral content by the total bone volume (bone and marrow).Biomechanical strength testing of femurs and lumbar vertebraesBiomechanical strength testing of right femurs and lumbar vertebrae was performed at the femur or lumbar vertebrae midpoint and femur or lumbar vertebrae neck as previously described (Ward and Fonseca, 2007). Femurs or lumbar vertebrae were soaked in physiological saline (9 g NaCl/L) for 4 h at room temperature prior to testing. Three-point bending at the femur or lumbar vertebrae midpoint and femur or lumbar vertebrae neck fracture was performed using a materials testing system and a specialized software program.Statistical analysesData obtained were expressed as mean ± SD (n = 8). The significance of differences between groups were determined by unpaired student t test using the InStat Statistical software (GraphPad Software Inc, San Diego, Calif). Differences with P < 0.05 were considered statistically significant.RESULTSCompared with normal control rats, blood ALP and IL-6 levels in model control rats were markedly increased. There was a significant increase in the plasma ALP and IL-6 levels when the MOP was administered to rats (Table 1).When compared with the normal control rats, femur and lumbar vertebrae density in model control group were significantly reduced. The MOP treatment for 3 months showed that femur and lumbar vertebrae density were significantly lower in the MOP-treated groups than those in model control group. Moreover, the levels increased with increasing dose of MOP (Figure 1).Compared with normal control rats, maximum load (N) of femur and lumbar vertebrae in model control rats was markedly decreased. Three months after MOP (200 and 400 mg/kg body weight) was orally administered to rats, the maximum load (N) of femur and lumbar vertebrae was significantly enhanced in a dose-dependent manner, as shown in Figure 2.DISCUSSIONM. officinalis have been widely used for thousands of years to treat fractures and joint diseases. The theory of Chinese medicine is to restore balances at all effective levels. Although the herbal medicines are considered as a cost-effective alternative by their traditional users, their international acceptance as an alternative therapeutic114 Int. J. Phys. Sci.Table 1. Effect of Morinda officinalis polysaccharides on blood ALP and IL-6 level.Group ALP (U/L) IL-6 (pg/ml)NC 146.33±7.31 19.78±1.09MC 199.36±8.04 b 31.47±2.05 bMOP I 180.84±8.34 d 26.04±1.35 cMOP II 156.31±7.93 d 20.32±1.47 db p<0.05, compared with group NC;c p<0.05,d p<0.01, compared with group MC.vertebrae density (g/cm2). b p<0.05, compared with group NC; d p<0.05,compared with group MC.(N) of femur and lumbar vertebrae. b p<0.05, compared with group NC; dp<0.01, compared with group MC.regime for prevention and treatment of osteoporosis will require extensive research using modern science (Thoo et al., 2010).The reduction of bone mass and predicted bone strength in aged rats was demonstrated in our study. The age-induced increase in bone resorption was confirmed by the increase in the serum level of ALP and IL-6. Alkaline phosphatase (ALP, ALKP) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The normal range is 20 to 140 IU/L (Trumble et al., 2008)./wiki/Alkaline_phosphatase High ALP levels can show that the bile ducts are blocked (Osathanon et al., 2009). Levels are significantly higher in children and pregnant women. Also, elevated ALP indicates that there could be active bone formation occurring as ALP is a by product of osteoblast activity (such as the case in Paget's disease of bone) (Withold et al., 1994). Our study revealed that aging appeared to stimulate the process of osteoclastogenesis in rats like increased serum level of ALP and IL-6. MOP treatment significantly reduced serum level of ALP and IL-6 in rats fed with MOP.The significant weight increase in aged rats has been reported in other experimental studies. These have noted the increase in body weight as a mechanism to provide an additional stimulus for bone neoformation, serving as a partial protection against the osteopenia which occurs in long bones due to supporting the body weight (Kalu, 1991; Notomi et al., 2003). In the present study, our work confirmed that aging can reduce bone density and bone strength, which was in agreement with above-mentioned reports. Moreover, we demonstrated for the first time that the M. officinalis polysaccharides prevent the reduction of bone mass and bone strength induced in aged rats.REFERENCESBattmann A, Battmann A, Jundt G, Schulz A (1997). Endosteal human bone cells (EBC) show age-related activity in vitro, Exp Clin Endocrinol. Diabetes, 105: 98–102.Beamer WG, Donahue LR, Rosen CJ, Baylink DJ (1998). Genetic variability in adult bone density among inbred strains of mice, Bone, 18: 397–403.Bloomfield SA, Hogan HA, Delp MD (2002). 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Contents
doi:10.1016/S0304-4238(05)00179-2Scientia Horticulturae 105 (2005) 533–536VOL. 105, ISSUE 130 MAY 2005Regular papersSensitivity of root system to low temperature appears to be associated with the root hydraulic properties through aquaporin activityS.H. Lee and G.C. Chung (Gwangju, South Korea). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1Growth and productivity of potato as influenced by cultivar and reproductive growth. I. Stomatal conductance, rate of transpiration, net photosynthesis, and dry matter production and allocationT. Tekalign and P.S. Hammes (Pretoria, South Africa). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13Growth and productivity of potato as influenced by cultivar and reproductive growth. II. Growth analysis, tuber yield and qualityT. Tekalign and P.S. Hammes (Pretoria, South Africa). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29Identification of Olea europaea L. cultivars using inter-simple sequence repeat markersP.J. Terzopoulos (Athens, Greece), B. Kolano (Katowice, Poland), P.J. Bebeli, P.J. Kaltsikes (Athens, Greece) and I. Metzidakis (Chania, Greece). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45Segregation patterns of several morphological characters and RAPD markers in interspecific hybrids between Dianthus giganteus and D. carthusianorumS.Y . Lee, B.W. Yae (Suwon, South Korea) and K.S. Kim (Seoul, South Korea). . . . . . . . . . . . . . . . 53Calcium translocation to fleshy fruit: its mechanism and endogenous controlM.C. Saure (Moisburg, Germany). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65Phenotypic variation in native walnut populations of Northern AlbaniaG. Zeneli, H. Kola and M. Dida (Tirana, Albania). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91Physiological acclimation of seashore paspalum and bermudagrass to low lightY . Jiang, R.N. Carrow (Griffin, GA, USA) and R.R. Duncan (San Antonio, TX, USA). . . . . . . . . . 101Somatic embryogenesis from floral tissues of feijoa (Feijoa sellowiana Berg)S. Stefanello (Toledo, Brazil), L.L.D. Vesco (Programa, Brazil), J.P.H.J. Ducroquet (Brazil),R.O. Nodari and M.P. Guerra (Florianópolis, Brazil). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117Temperature effects on corm dormancy and growth of Zephyra elegans D.DonP. Yañez, H. Ohno and K. Ohkawa (Shizuoka City, Japan). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127Short communicationsImproved technique for counting chromosomes in almondP. Martínez-Gómez, R. Sánchez-Pérez (Espinardo (Murcia), Spain), Y . Vaknin (Davis, CA, USA),F.Dicenta (Espinardo (Murcia), Spain) and T.M. Gradziel (Davis, CA, USA). . . . . . . . . . . . . . . . . 139Contents of Scientia HorticulturaeVolume 105 (2005)Growth responses and endogenous IAA and iPAs changes of litchi (Litchi chinensis Sonn.) seedlings induced by arbuscular mycorrhizal fungal inoculationQ. Yao, H.H. Zhu and J.Z. Chen (Guangzhou, China). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145 Guide for Authors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153 VOL. 105, ISSUE 210 JUNE 2005 Regular papersEvaluation and modelling of greenhouse cucumber-crop transpiration under high and low radiation conditionsE. Medrano, P. Lorenzo, M.C. Sánchez-Guerrero (Almería, Spain) and J.I. Montero(Cabrils, Spain). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 163 Growth, yield, fruit quality and nutrient uptake of hydroponically cultivated zucchini squash as affected by irrigation systems and growing seasonsY. Rouphael and G. Colla (Viterbo, Italy). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177Net CO2exchange rate of in vitro plum cultures during growth evolution at different photosynthetic pho-ton flux densityS. Morini and M. Melai (Pisa, Italy). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 197 Leaf phenolic content of pear cultivars resistant or susceptible to fire blightY. Gunen, A. Misirli and R. Gulcan (Izmir, Turkey). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 213 Influences of cold deprivation during dormancy on carbohydrate contents of vegetative and floral primordia and nearby structures of peach buds (Prunus persica L. Batch)M. Bonhomme, R. Rageau, A. Lacointe (Clermont-Ferrand, France) and M. Gendraud(Aubière, France). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 223 Inheritance and expression of fruit texture melting, non-melting and stony hard in peach T. Haji, H. Yaegaki and M. Yamaguchi (Ibaraki, Japan). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 241 Organic acid analysis and plant water status of two Aechmea cultivars grown under greenhouse condi-tions: implications on leaf qualityE. Londers, J. Ceusters, I. Vervaeke (Heverlee, Belgium), R. Deroose (Evergem, Belgium) and M.P.De Proft (Heverlee, Belgium). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 249 DNA ploidy level of colchicine-treated hops (Humulus lupulus L.)A. Koutoulis, A.T. Roy, A. Price, L. Sherriff and G. Leggett (Tasmania, Australia). . . . . . . . . . . . . 263 Nutrient solution effects on the development and yield of Anthurium andreanum Lind. in tropical soilless conditionsL. Dufour (Petit-Bourg, France) and V. Guérin (Beaucouzé, France). . . . . . . . . . . . . . . . . . . . . . . . 269 Short communicationIdentification of persimmon (Diospyros kaki) cultivars and phenetic relationships between Diospyros species by more effective RAPD analysisM. Yamagishi, S. Matsumoto, A. Nakatsuka and H. Itamura (Shimane, Japan). . . . . . . . . . . . . . . . 283 VOL. 105, ISSUE 3 4 JULY 2005 Regular papersEffects of fruit shape and plant density on seed yield and quality of squashH. Nerson (Ramat Yishay, Israel). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 293 Yield of scarlet eggplant (Solanum aethiopicum L.) as influenced by planting date of companion cowpea K. Ofori (Legon, Ghana) and D.K. Gamedoagbao (Bunso, Ghana). . . . . . . . . . . . . . . . . . . . . . . . . 305 Effective pollination period estimation in olive (Olea europaea L.): a pollen monitoring applicationF. Orlandi, B. Romano and M. Fornaciari (Perugia, Italy). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 313 Decreased anthocyanin biosynthesis in grape berries grown under elevated night temperature condition K. Mori, S. Sugaya and H. Gemma (Ibaraki, Japan). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 319 534Contents of Volume 105Contents of Volume 105535 Promotion of seed germination and subsequent seedling growth of loquat (Eriobotrya japonica, Lindl)by moist-chilling and GA3applicationsE.-R.F.A. El-Dengawy (El-Mansoura, Egypt). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 331 Arsenic as a factor affecting virus infection in tomato plants: changes in plant growth, peroxidase activ-ity and chloroplast pigmentsE. Miteva, D. Hristova (Kostinbrod, Bulgaria), V. Nenova (Sofia, Bulgaria) and S. Maneva(Kostinbrod, Bulgaria). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 343 Agrobacterium-mediated transformation of Musa acuminata cv. “Grand Nain” scalps by vacuum infiltrationP.O.M. Acereto-Escoffié, B.H. Chi-Manzanero, S. Echeverría-Echeverría, R. Grijalva, A.J. Kay,T. González-Estrada, E. Castaño and L.C. Rodríguez-Zapata (Yucatán, México). . . . . . . . . . . . . . . 359 Modeling the mass of apples by geometrical attributesA. Tabatabaeefar and A. Rajabipour (Karaj, Iran). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 373 Effects of cold storage on postharvest leaf and flower quality of potted Oriental-, Asiatic- and LA-hybrid lily cultivarsA.P. Ranwala and W.B. Miller (Ithaca, NY, USA). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 383 Enhanced thermotolerance of the vegetative part of MT-sHSP transgenic tomato lineP.C. Nautiyal (Junagadh, India), M. Shono and Y. Egawa (Okinawa, Japan). . . . . . . . . . . . . . . . . . 393 Short communicationInduction of somatic embryogenesis in lotus (Nelumbo nucifera Geartn.)S. Arunyanart (Bangkok, Thailand) and M. Chaitrayagun (Phuket, Thailand). . . . . . . . . . . . . . . . . 411 VOL. 105, ISSUE 429 JULY 2005 Regular papersAerial tubers induced in turnip (Brassica rapa L. var. rapa(L.) Hartm.) by gibberellin treatment T. Nishijima, H. Sugii, N. Fukino and T. Mochizuki (Kusawa, Japan). . . . . . . . . . . . . . . . . . . . . . . 423 Changes during the ripening of the very late season Spanish peach cultivar Calanda. Feasibility of using CIELAB coordinates as maturity indicesA. Ferrer, S. Remón, A.I. Negueruela and R. Oria (Zaragoza, Spain). . . . . . . . . . . . . . . . . . . . . . . . 435 Involvement of cell proliferation and cell enlargement in increasing the fruit size of Malus species T. Harada, W. Kurahashi (Hirosaki, Japan), M. Yanai (Rokkasho, Japan), Y. Wakasa(Tsukuba, Japan) and T. Satoh (Kuroishi, Japan). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 447 Yield, fruit quality, and tree health of ‘Allen Eureka’ lemon on seven rootstocks in Saudi ArabiaA. Al-Jaleel (Najran, Saudi Arabia), M. Zekri (LaBelle, FL, USA) and Y. Hammam(Najran, Saudi Arabia). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 457 Effect of temperature on seed and fruit development in three mango (Mangifera indica L.) cultivars N. Sukhvibul (Chiang Rai, Thailand), A.W. Whiley and M.K. Smith (Nambour, Australia). . . . . . . 467 Factors affecting tissue culture of Damask rose (Rosa damascena Mill.)Z. Jabbarzadeh and M. Khosh-Khui (Fars, Iran). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 475 Alterations in endogenous polyamines in bulbs of tuberose (Polianthes tuberosa L.) during dormancy S. Sood and P.K. Nagar (Himachal Pradesh, India). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 483 Phenological growth stages of the cherimoya tree (Annona cherimola Mill.)R. Cautín (Quillota, Chile) and M. Agustí (Valencia, Spain). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 491 The influence of exogenous ethylene on growth and photosynthesis of mustard (Brassica juncea) following defoliationN.A. Khan (Aligarh, India). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 499 Short communicationsIsolation and characterization of a new d-limonene synthase gene with a different expression pattern in Citrus unshiu MarcT. Shimada, T. Endo, H. Fujii and M. Omura (Shizuoka, Japan). . . . . . . . . . . . . . . . . . . . . . . . . . . 507536Contents of Volume 105Metabolic stability of plants regenerated from cryopreserved shoot tips of Dioscorea deltoidea– an endangered medicinal plantS. Dixit-Sharma (Bangalore, India), S. Ahuja-Ghosh (Charlottesville, V A, USA), B. Bushan Mandaland P.S. Srivastava (New Delhi, India). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 513 Author Index Scientia Horticulturae Volume 105. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 519 Subject Index Scientia Horticulturae Volume 105. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 523 Contents of Scientia Horticulturae Volume 105. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 533。
CJJT 187-2012 建设电子档案元数据标准
2英文名称：宜采用元素或子元素的英文表达，多个英文 字中间不应留空格，每个英文字的首个字母应大写。
3目的：应规定元素的用途。 4使用：应规定元素的使用方法，并应符合下列规定：
本标准由住房和城乡建设部负责管理，由住房和城乡建设部 城建档案工作办公室负责具体技术内容的解释。执行过程中如有 意见或建议，请寄送住房和城乡建设部城建档案工作办公室（地 址：北京市海淀区三里河路9号，邮政编码：100835）0
本标准主编单位：住房和城乡建设部城建档案工作办 公室 珠海市城市建设档案馆
文件系列 Records Series
由逻辑上联系、物理上分离的若干文件组合聚合而 成的文件管理单元
文件集 Records Set
由一个或若干个具有内在联系的职能活动所形成的 全部文件的集合组成的文件管理单元
4.2.3文件标识应为同一文件管理系统中文件实体的唯一标识, 其属性应符合表4. 2. 3的规定。当子元素文件标识码采用多种方 案标识时，应与文件标识方案同时使用。
6
编号
Ml
表4. 2. 2-1文件层级属性
英文名称
RecordCategory
1便于组织与管理文件管理系统中各类活动过程中形成的电子文件 。 冃的
2用于关联文件与形成文件的背景信息
适用性
使用条件
可选性
使用 应与形成文件实体的业务层级、
文件实休 责任者层级相关联
必选
可重 复性
否
包含 子元素
否
取值
取值类型 字符串
中华人民共和国反倾销(英文版)
反倾销条例（英文版）Anti-dumping Regulation of T he People’s Republic of ChinaDecree [2001] No.328 of the state CouncilThe Anti-Dumping Regulation of the People’’s Republic of China, which were adopted at the 46th executive meeting of the State Council on October 31, 2001, are hereby promulgated, and shall come into force on January 1, 2001. Premier of the State Council: Zhu RongjiNovember 26, 2001Attachment:Anti-dumping Regulation of the People’’s Republic of ChinaChapter I General ProvisionsArticle 1The present Regulation has been enacted in accordance with the relevant provisions of the Foreign Trade Law of the People’’s Republic of China with a view to maintaining foreign trade order and fair competition.Article 2In case that imported products enter the market of the People’’s Republic of China by way of dumping, and cause material damage or constitute a threat of material damage to an already established domestic industry, or cause a material impediment to the establishment of a domestic industry, an investigation shall be conducted and anti-dumping measures shall be taken in accordance with the present Regulation.Chapter II Dumping and DamageArticle 3The term "dumping" shall refer to the entry of imported products into the market of the Peo ple’’s Republic of China, in the ordinary course of trade, with their export price lower than their normal value. The Ministry of Foreign Trade and Economic Cooperation (hereinafter referred to as the MOFTEC) shall be responsible for the investigation on and determination of dumping.Article 4The normal value of imported products shall be determined in the following ways on the basis of different circumstances: (1) in case that the products of the same category as that of the imported products have a comparable price in the domestic market of the exporting country (region) in the ordinary course of trade, that comparable price shall be the normal value;(2) in case that the products of the same category as that of the imported products are not sold on the domestic market of the exporting country (region) in the ordinary course of trade, or the price or quantity of the products of the same category cannot be used as a basis for fair comparison, the normal value shall be the comparable price at which the products of the same category are exported to a proper third country (region) or shall be the production cost of the products of the same category in the country (region) of origin plus reasonable expenses and profits.Where the imported products do not directly come from the country (region) of origin, the normal value shall be determined in accordance with Item (1) of the preceding paragraph; however, if the products are transported only through the exporting country (region) or the products are not produced in the exporting country (region) or no comparable price exists in the exporting country (region), etc., the price of the said products of the same category in the country (region) of origin may be regarded as the normal value.Article 5The export price of the imported products shall be determined in the following ways according to different circumstances: (1) in case the imported products have an actual payment price or a payable price, such price shall be the export price;(2) in case the imported products do not have an export price or its price is not reliable, the price presumed on the basis of the price at which the imported products are re-sold for the first time to an independent buyer shall be regarded as the export price; however, if the imported products are not re-sold to an independent buyer or not re-sold in the status when they are imported, the price presumed by the MOFTEC on a reasonable basis may be regarded as the export price. Article 6The margin between the export price of imported products which is lower than their normal value shall be the dumping margin.The export price of the imported products and the normal value shall be compared in a fair and reasonable manner by taking the various comparable factors which may impact the price into consideration.The dumping margin shall be determined with the weighted average normal value and the weighted average price of all the comparable export transactions being compared, or with the normal value and the export price being compared transaction by transaction.Where the export prices are considerably different between different buyers, regions or periods, and thus are difficult to be compared in the ways provided in the preceding paragraph, the weighted average normal value may be compared with the price of a single export transaction.Article 7The term "damage" shall refer to the fact that dumping has caused material damage or constitute a threat of material damage to an already established domestic industry, or caused a material impediment to the establishment of a domestic industry.The State Economic and Trade Commission (hereinafter referred to as the SETC) shall be responsible for the investigation on and determination of damage; while the anti-dumping investigation on the damage to a domestic industry relating to agricultural products shall be conducted by the SETC in collaboration with the Ministry of Agriculture.Article 8When determining the damage caused to a domestic industry by dumping, the following items shall be examined:(1) the quantity of the dumped imports, including a sharp increase in the absolute quantity of the dumped imports or the quantity as compared with the production or consumption of the domestic products of the same category, or the possibility of a sharp increase in the quantity of the dumped imports;(2) the price of the dumped imports, including the price cuts of the dumped imports or the impacts such as great restraint or reduction, etc. to the price of the domestic products of the same category;(3) the impacts of the dumped imports upon the relevant economic factors and targets of the domestic industry;(4) the production capacity, export capability of the exporting country (region) and the country (region) of origin on the dumped imports as well as the inventory of the investigated products;(5) other factors which cause damage to the domestic industry. The threat of material damage shall be determined on the basis of the facts instead of the accusation, presumption or minor possibility.The damage caused by dumping to a domestic industry shall be determined on the basis of affirmative evidence, and no factor other than dumping which causes damage shall be attributed to dumping.Article 9Where the dumped imports come from two or more countries (regions), and meanwhile meet the following conditions, a cumulative evaluation may be conducted upon the impacts caused by the dumped imports to a domestic industry:(1) the dumping margin of the dumped imports from each country (region) shall be no lower than 2%, and the import volume of the products may not be negligible;(2) it is proper to conduct a cumulative evaluation on the basis of the competition conditions between the dumpedimports as well as between the dumped imports and the domestic products of the same category.The term "to be negligible" shall refer to the fact that the proportion of the quantity of the dumped imports from a country (region) to the total import volume of products of the same category shall be lower than 3%; except where the total import volume lower than 3% from some countries (regions) exceeds 7% of the total import volume of the products of the same category.Article 10The evaluated impacts on the dumped imports shall be separately determined with regard to the production of the domestic products of the same category; where they may not be separately determined with regard to the production of the domestic products of the same category, the production of the narrowest product group or scope which includes the products of the same category shall be examined.Article 11Th e term "domestic industry" shall refer to all the manufacturers within the People’’s Republic of China of the domestic products of the same category or the manufacturers within the People’’s Republic of China whose total output accounts for the major part of the aggregate output of the domestic products of the same category; however, if a domestic manufacturer is associated with an export business operator or import business operator, or he himself is an import business operator of the dumped imports, he may be excluded from the domestic industry.Where, under particular circumstances, the domestic manufacturers in a regional market sell the whole or nearly the whole of the products of the same category in the said market, and the products of the same category in the said market are not mainly supplied by the domestic manufacturers of other places, they may be regarded as a separate industry. Article 12The term "products of the same category" shall refer to the products identical to the dumped imports; where there are no identical products, the products which are most similar to the features of the dumped imports shall be the products of the same category.Chapter III Anti-Dumping InvestigationsArticle 13A domestic industry or a natural person, legal person or relevant organization representing a domestic industry (hereinafter uniformly referred to as the applicant) may file a written application on anti-dumping investigation to the MOFTEC in accordance with these Rules.Article 14The application letter shall contain the following contents:(1) name, address and relevant information of the applicant;(2) complete specifications on the imported products under application for investigation, including product name, the exporting country (region) or country (region) of origin involved, the known export business operator or manufacturer, information on the price of the products when they are consumed in the domestic market of the exporting country (region) or country (region) of origin, information on export price, etc.;(3) a statement on the quantity and value of the domestic products of the same category;(4) the impacts of the quantity and price of the imported products under application for investigation on the domestic industry;(5) other contents which the applicant considers it necessary to state.Article 15The application letter shall be attached with the following evidence:(1) the existence of dumping of the imported products under application for investigation;(2) the damage to domestic industry;(3) the causal link between the dumping and damage.Article 16The MOFTEC shall, within 60 days as of its receipt of the application letter and the relevant evidence submitted by the applicant, examine whether the application is filed by the domestic industry or filed by representing the domestic industry, the contents of the application letter and the evidence attached to it, etc., and shall, upon consultation with the SETC, decide to initiate an investigation or not.The MOFTEC shall, before deciding to initiate an investigation, notify the government of the relevant exporting country (region).Article 17Where, among the manufacturers in a domestic industry who are in favor or disfavor of the application, the output of those who are in favor accounts for 50% or more of the total output of those who are in favor and disfavor, it shall be deemed that the application is filed by the domestic industry or filed by representing the domestic industry, and the anti-dumping investigation may be initiated; however, if the output of the domestic manufacturers who support the application is less than 25% of the total output of the domestic products of the same category, the anti-dumping investigation shall not be initiated.Article 18Where, under particular circumstances, the MOFTEC does not accept the written application for anti-dumping investigation, but has sufficient evidence to believe that there exist a dumping and damages and there is a causal link between the dumping and damages, it may, upon consultation with the SETC, decide to initiate an investigation.The MOFTEC or the SETC is hereinafter uniformly referred to as the investigation organ.Article 19The decisions on initiating an investigation shall be announced by the MOFTEC, and shall be notified to the applicant, the known export business operators and import business operators, the government of the exporting country (region) as well as other interested organizations or individuals (hereinafter uniformly referred to as the interested parties).Once the decisions on initiating an investigation is announced, the MOFTEC shall provide the known export business operators and the government of the exporting country (region) with a copy of the application letter.Article 20The investigation organ may obtain information from the interested parties and conduct the investigation by means of questionnaires, samples, hearings and on-the-spot checks, etc..The investigation organ shall provide the relevant interested parties with opportunities for stating their viewpoints and grounds of argument.The MOFTEC may, when considering it necessary, send functionaries to the relevant country (region) for conducting the investigation, except where the relevant country (region) concerned objects to the investigation.Article 21When the investigation organ is conducting an investigation, the interested parties shall tell the truth and provide relevant materials. Where the interested parties fail to tell the truth or fail to provide relevant materials, or fail to provide necessary information within a reasonable time limit, or seriously hamper the investigation in other forms, the investigation organ may make an adjudication on the basis of the already obtained facts and the best available information.Article 22Where the interested parties consider that the divulgence of the materials provided by them will cause seriously bad effects, they may apply to the investigation organ for treating the materials as confidential.Where the investigation organ considers the application for confidentiality is justified, it shall treat the materialsprovided by the interested parties as confidential, and meanwhile request the interested parties to provide a copy of non-confidential outline of the materials.The materials treated as confidential materials shall not be divulged without the consent of the interested parties who provide them.Article 23The investigation organ shall permit the applicant and the interested parties to have access to the relevant materials of the case, except where the materials are treated as confidential.Article 24The MOFTEC and the SETC shall, upon the investigation result, make separate an initial award on dumping and damage as well as on whether the causal link between the dumping and damage is tenable, which shall be announced by the MOFTEC.Article 25Where the initial award affirms the dumping and damage as well as the causal link between the dumping and damage, the MOFTEC and the SETC shall continue the investigation on the dumping, the dumping margin, the damage and its extent, and shall make separate a final award upon the investigation result, which shall be announced by the MOFTEC.Before the making of the final award, the MOFTEC shall notify all the known interested parties of the basic facts upon which the final award is made.Article 26An anti-dumping investigation shall be ended within 12 months as of the date of announcement of the decision on initiating the investigation; under particular circumstances, the time limit may be extended, provided that the extension shall not exceed 6 months.Article 27Under any of the following circumstances, the anti-dumping investigation shall be terminated and be announced by the MOFTEC:(1) the applicant revokes the application;(2) there is not enough evidence to prove the existence of dumping, damage or the causal link between the dumping and damage;(3) the dumping margin is lower than 2%;(4) the actual or potential import volume of the dumped imports or the damage is negligible;(5) the MOFTEC and the SETC both consider it is not appropriate to continue the anti-dumping investigation.Where the investigated products from one or more countries (regions) are under any of the circumstances listed in Items (2), (3), and (4) of the preceding paragraph, the anti-dumping investigation with regard to the involved products shall be terminated.Chapter IV Anti-Dumping MeasuresSection 1 Provisional Anti-Dumping MeasuresArticle 28Where the initial award affirms the dumping and the consequent damage to a domestic industry, the following provisional anti-dumping measures may be taken:(1) to levy provisional anti-dumping tariffs;(2) to request the provision of cash deposits, guaranty letter or other forms of guaranty. The amount of the provisional anti-dumping tariffs, the cash deposits, the guaranty letter and other forms of guaranty shall not exceed the dumping margin ascertained in the initial awards.Article 29The levy of the provisional anti-dumping tariffs shall be proposed by the MOFTEC and be decided on by the Tariff Policy Committee under the State Council upon the proposition of the MOFTEC, and shall be announced by the MOFTEC. The request for the provision of cash deposits, guaranty letter or other forms of guaranty shall be decided on and announced by the MOFTEC. The customs shall implement the decision as of the date provided in the announcement. Article 30The time limit for the provisional anti-dumping measures shall not exceed 4 months as of the date of entry into force of the announcement of the decisions on provisional anti-dumping measures; however, such time limit may be extended to 9 months under particular circumstances.No provisional anti-dumping measure shall be taken within 60 days as of the date when the decisions on initiating an anti-dumping investigation is announced.Section 2 Pricing CommitmentsArticle 31The export business operators of the dumped imports may, during the period of anti-dumping investigation, make pricing commitments to the MOFTEC on changing the price or ceasing export at a dumping price.The MOFTEC may propose suggestions on pricing commitments to the export business operators.The investigation organ may not force the export business operators to make pricing commitments.Article 32The export business operators’’ refusal to make pricing commitments or to accept the suggestions on pricing commitments shall not hamper the investigation of and determination on the anti-dumping cases. Where the export business operators continue dumping the imported products, the investigation organ shall have the right to determine that the threat of damage is more possible to arise.Article 33Where the MOFTEC considers the pricing commitments made by the export business operators are acceptable, it may, upon consultation with the SETC, decide to suspend or terminate the anti-dumping investigations, instead of taking any provisional anti-dumping measure or levying anti-dumping tariffs. The decisions on suspending or terminating the anti-dumping investigation shall be announced by the MOFTEC.Where the MOFTEC does not accept the pricing commitments, it shall state the reason to the relevant export business operators.The investigation organ shall not, before making an affirmative initial award on dumping and damage caused therefrom, seek or accept pricing commitments.Article 34After suspending or terminating the anti-dumping investigation in accordance with Paragraph 1 ofArticle 33 of the present Regulation, the investigation organ may, upon request by the export business operators or if considering it necessary, continue investigating the dumping and damage.Upon the investigation result as mentioned in the preceding paragraph, if a negative award on dumping or damageis made, the pricing commitments shall automatically become invalid; while if an affirmative award on dumping or damage is made, the pricing commitments shall continue to be valid.Article 35The MOFTEC may require the export business operators to regularly provide the relevant information and materials for implementing the pricing commitments, and may verify such information and materials.Article 36In case any export business operator violates its pricing commitments, the MOFTEC may, upon consultation with the SETC, immediately decide to resume the anti-dumping investigation in accordance with the present Regulation; and may,upon the best available information, decide to take the provisional anti-dumping measures, as well as retrospect to the levy of the anti-dumping tariffs on the products imported within 90 days before the provisional anti-dumping measures were taken, except where the products were imported before the pricing commitments are violated.Section 3 Anti-dumping tariffsArticle 37Where the final adjudication decisions establish the dumping and the consequent damage caused to the domestic industry, the anti-dumping tariffs may be levied.Article 38The levy of the anti-dumping tariffs shall be proposed by the MOFTEC and be decided on by the Tariff Policy Committee under the State Council upon the proposition of the MOFTEC, and shall be announced by the MOFTEC. The customs shall execute such levy as of the date provided in the announcement.Article 39The anti-dumping tariffs shall be applicable to the products imported after the final award has been announced, exceptfor the circumstances provided inArticle s 36, 43 and 44 of the present Regulation.Article 40The taxpayers of the anti-dumping tariffs shall be the import business operators of the dumped imports.Article 41The anti-dumping tariffs shall be separately determined on the basis of the dumping margins of different export business operators. Where the anti-dumping tariffs needs to be levied upon the dumped imports of the export business operators beyond the scope of examination, the applicable anti-dumping tariffs shall be determined in a reasonable method.Article 42The amount of anti-dumping tariffs shall not exceed the dumping margin determined in the final adjudication decision. Article 43Where the final award affirms the existence of the material damage, and prior to which a provisional anti-dumping measure has been taken, the anti-dumping tariffs may be levied in retrospect to the period of the provisional anti-dumping measure.Where the final award affirms the existence of the threat of material damage, and a provisional anti-dumping measure has been taken under the circumstance that an adjudication of material damage will be made if no provisional anti-dumping measure has been taken in advance, the anti-dumping tariffs may be levied in retrospect to the period of the provisional anti-dumping measure.Where the anti-dumping tariffs determined in the final award is higher than the paid or payable provisional anti-dumping tariffs or the amount valuated for the sake of guaranty, the difference shall not be collected; where it is lower than the paid or payable provisional anti-dumping tariffs or the amount valuated for the sake of guaranty, the difference shall be refunded upon the specific circumstance or the amount of the duty shall be re-calculated.Article 44Where the following two circumstances coexist, the anti-dumping tariffs may be retrospectively levied upon the products imported within 90 days before the provisional anti-dumping measures were taken, except for the products imported prior to the initiation of the investigation:(1) the dumped imports has a record of dumping causing damage to the domestic industry, or the import business operators of the products know or ought to know that the export business operators are dumping products and that dumping would lead to damage to domestic industry;(2) the dumped imports are massively imported within a short period, and are possible to seriously destroy the remedialeffect of the anti-dumping tariffs to be levied immediately.Article 45Where the final award determines not to levy the anti-dumping tariffs or not to retrospectively levy the anti-dumping tariffs, the levied provisional anti-dumping tariffs and the collected cash deposits shall be refunded, and the guaranty letter or other forms of guaranty shall be cancelled.Article 46Where an import business operator of dumped imports has evidence to prove that the amount of paid anti-dumping tariffs exceeds the dumping margin, he may apply to the MOFTEC for refund of the tariffs levied; after the MOFTEC has examined and verified the application and proposed the refund, the Tariff Policy Committee under the State Council may, upon the proposition of the MOFTEC, make the decision on the refund, and the customs shall execute the refund. Article 47Where, after the anti-dumping tariffs has been levied upon the imported products, a new export business operator who has not exported such products to the People’’s Republic of China within the period of investigation but could prove the irrelevance between he himself and the export business operator against who anti-dumping tariffs were levied, he may apply to the MOFTEC for separate determination of the dumping margin. The MOFTEC shall make a rapid examination and make a final award. It may, during the period of examination, take the measures provided in Item (2) of Paragraph 1 ofArticle 28 of the present Regulation, provided it shall not levy the anti-dumping tariffs upon these products.Chapter V Time Limit for and Re-examination of Anti-dumping tariffs and Pricing CommitmentsArticle 48Neither the time limit for levying the anti-dumping tariffs nor that for implementing the pricing commitments shall exceed 5 years; however, where it is re-examined and determined that the termination of the levy of the anti-dumping tariffs is possible to lead to the continuance or re-occurrence of the dumping or damage, the time limit for levying the anti-dumping tariffs may be appropriately extended.Article 49After the anti-dumping tariffs has taken effect, the MOFTEC may, with a justifiable reason and upon consultation with the SETC, decide to re-examine the necessity of continuing the levying of anti-dumping tariffs; it may also, after a reasonable period of time, upon the request of the interested parties and after having examined the corresponding evidence provided by the interested parties, decide to re-examine the necessity of continuing the levying of anti-dumping tariffs.After the pricing commitments have taken effect, the MOFTEC may, with a justifiable reason, decide to re-examine the necessity of continuing to implement the pricing commitments; it may also, after a reasonable period of time, upon the request of the interested parties and after having examined the corresponding evidence provided by the interested parties, decide to re-examine the necessity of the continuing to implement the pricing commitments.Article 50The reservation, amendment or cancellation of the anti-dumping tariffs shall be proposed by the MOFTEC upon the re-examination result and in accordance with the present Regulation, shall be decided by the Tariff Policy Committee under the State Council upon the proposition of the MOFTEC, and shall be announced by the MOFTEC. The MOFTEC may also, in accordance with the present Regulation and upon consultation with the SETC, make the decision on reserving, amending or canceling the pricing commitments and shall announce such decision.Article 51The re-examination procedures shall be followed with reference to the relevant provisions in the present Regulation on anti-dumping investigations.The time limit for re-examination shall not exceed 12 months, commencing from the date when the re-examination。
香港会计准则
MEMBERS' HANDBOOKCONTENTS OF VOLUME II(Updated to May 2017)Issue/(Reviewdate) PREFACE AND FRAMEWORKPREFACE Preface to Hong Kong Financial Reporting Standards .....................................10/06(4/15)CONCEPTUALFRAMEWORKConceptual Framework for Financial Reporting ................................................10/10HONG KONG ACCOUNTING STANDARDS (HKAS)HKAS 1RevisedPresentation of Financial Statements................................................................12/07(1/17) HKAS 2 Inventories .........................................................................................................3/04(2/14) HKAS 7 Statement of Cash Flows ..................................................................................12/04(6/16) HKAS 8 Accounting Policies, Changes in Accounting Estimates and Errors .................9/04(2/14) HKAS 10 Events after the Reporting Period .....................................................................3/04(2/14) HKAS 11 Construction Contracts ......................................................................................12/04(3/10) HKAS 12 Income Taxes ....................................................................................................11/04(6/16) HKAS 16 Property, Plant and Equipment .........................................................................11/05(1/17) HKAS 17 Leases ...............................................................................................................12/04(1/17) HKAS 18 Revenue ............................................................................................................11/04(5/14) HKAS 19(2011)Employee Benefits.............................................................................................7/11(12/16)HKAS 20 Accounting for Government Grants and Disclosure of GovernmentAssistance ...................................................................................................12/04(2/14) HKAS 21 The Effects of Changes in Foreign Exchange Rates ........................................3/04(5/14) HKAS 23RevisedBorrowing Cost……………………………….......................................................6/07(1/17)HKAS 24RevisedRelated Party Disclosures .................................................................................11/09 (11/16) HKAS 26 Accounting and Reporting by Retirement Benefit Plans ...................................8/04HKAS 27(2011)Separate Financial Statements .........................................................................6/11(1/17)HKAS 28 (2011) Investments in Associates and Joint Ventures ..................................................6/11(1/17)HKAS 29 Financial Reporting in Hyperinflationary Economies .....................................3/04(4/10) HKAS 32 Financial Instruments: Presentation ...............................................................11/04(11/14) HKAS 33 Earnings per Share ........................................................................................3/04(5/14) HKAS 34 Interim Financial Reporting ............................................................................10/04(12/16) HKAS 36 Impairment of Assets .....................................................................................8/04(1/17) HKAS 37 Provisions, Contingent Liabilities and Contingent Assets ..............................11/04(11/16) HKAS 38 Intangible Assets ............................................................................................8/04(1/17) HKAS 39 Financial Instruments: Recognition and Measurement ..................................1/06(11/16) HKAS 40 Investment Property .......................................................................................11/05(4/17) HKAS 41 Agriculture ......................................................................................................12/04(1/17)HONG KONG FINANCIAL REPORTING STANDARDS (HKFRS)First-time Adoption of Hong Kong Financial Reporting Standards ...............12/08(1/17) HKFRS 1RevisedHKFRS 2 Share-based Payment ...................................................................................4/04(11/16) HKFRS 3Business Combinations ..................................................................................3/08(11/16) RevisedHKFRS 4 Insurance Contracts .......................................................................................3/06(01/17) HKFRS 5 Non-current Assets Held for Sale and Discontinued Operations ...................8/04(12/16) HKFRS 6 Exploration for and Evaluation of Mineral Resources ....................................2/05(2/10) HKFRS 7 Financial Instruments: Disclosures ................................................................9/05(12/16) HKFRS 8 Operating Segments .....................................................................................3/07(12/16) HKFRS 9 Financial Instruments .....................................................................................11/09 (09/14) HKFRS 9 Financial Instruments (Hedge Accounting) ...................................................12/13 Financial Instruments .....................................................................................09/14 HKFRS 9(2014)HKFRS 10 Consolidated Financial Statements ...............................................................6/11(1/17) HKFRS 11 Joint Arrangements ........................................................................................6/11(12/16) HKFRS 12 Disclosure of Interests in Other Entities .........................................................6/11(1/17) HKFRS 13 Fair Value Measurement ................................................................................6/11(11/16) HKFRS 14 Regulatory Deferral Accounts ........................................................................2/14(1/17) HKFRS 15 Revenue from Contracts with Customers ......................................................7/14(6/16) HKFRS 16 Leases ............................................................................................................5/16 Annual Improvements to HKFRSs 2014-2016 Cycle.....................................3/17 ANNUALIMPROVEMENTSHONG KONG (IFRIC) INTERPRETATIONS (HK(IFRIC)-Int)HK(IFRIC)-Int 1 Changes in Existing Decommissioning, Restoration and Similar Liabilities ......8/04(7/10) HK(IFRIC)-Int 2 Members’ Shares in Co-operative Entities and Similar Instruments .................2/05(2/14) HK(IFRIC)-Int 4 Determining whether an Arrangement contains a Lease ..................................2/05(2/14) HK(IFRIC)-Int 5 Rights to Interests arising from Decommissioning, Restoration andEnvironmental Rehabilitation Funds ............................................................2/05(2/14) HK(IFRIC)-Int 6 Liabilities arising from Participating in a Specific Market – WasteElectrical and Electronic Equipment .............................................................9/05 HK(IFRIC)-Int 7 Applying the Restatement Approach under HKAS 29 Financial Reportingin Hyperinflationary Economies...................................................................1/06(7/10) HK(IFRIC)-Int 9 Reassessment of Embedded Derivatives .........................................................5/06(2/14) HK(IFRIC)-Int 10 Interim Financial Reporting and Impairment ....................................................9/06(7/12) HK(IFRIC)-Int 12 Service Concession Arrangements ...................................................................3/07(1/17) HK(IFRIC)-Int 13 Customer Loyalty Programmes .........................................................................9/07(2/14) HK(IFRIC)-Int 14 HKAS 19 —The Limit on a Defined Benefit Asset, Minimum Funding9/07(11/16) Requirements and their Interaction ..............................................................HK(IFRIC)-Int 15 Agreements for the Construction of Real Estate ...............................................8/08(8/10) HK(IFRIC)-Int 16 Hedges of a Net Investment in a Foreign Operation .........................................8/08(5/14) HK(IFRIC)-Int 17 Distributions of Non-cash Assets to Owners .....................................................12/08(5/14) HK(IFRIC)-Int 18 Transfers of Assets from Customers .................................................................2/09(8/10) HK(IFRIC)-Int 19 Extinguishing Financial Liabilities with Equity Instruments ...............................12/09(5/14) HK(IFRIC)-Int 20 Stripping Costs in the Production Phase of a Surface Mine 11/11 HK(IFRIC)-Int 21 Levies 6/13 HONG KONG INTERPRETATIONS (HK-Int)*HK-Int 4 Leases – Determination of the Length of Lease Term in respect of HongKong Land Leases .......................................................................................6/06 (12/09) HK-Int 5 Presentation of Financial Statements – Classification by the Borrower ofa Term Loan that Contains a Repayment on Demand Clause ....................11/10Note: * With effect from 24 May 2005, all Interpretations that are developed locally by the Institute are named Hong Kong Interpretations.HONG KONG (SIC) INTERPRETATIONS (HK(SIC)-Int)HK(SIC)-Int 10 Government Assistance – No Specific Relation to Operating Activities ..........12/04(8/10) HK(SIC)-Int 15 Operating Leases – Incentives .........................................................................12/04(9/10) HK(SIC)-Int 25 Income Taxes – Changes in the Tax Status of an Enterprise or itsShareholders ................................................................................................12/04(8/10) HK(SIC)-Int 27 Evaluating the Substance of Transactions Involving the Legal Form of aLease ............................................................................................................12/04(9/10)HK(SIC)-Int 29 Service Concession Arrangements: Disclosures ..............................................12/04(8/10) HK(SIC)-Int 31 Revenue – Barter Transactions Involving Advertising Services .......................12/04(5/14) HK(SIC)-Int 32 Intangible Assets – Web Site Costs ..................................................................12/04(5/14)GLOSSARY Glossary of Terms Relating to Hong Kong Financial Reporting Standards ......... 3/08(11/14) HONG KONG FINANCIAL REPORTING STANDARD FOR PRIVATEENTITIES (HKFRS-PE)HKFRS-PE HONG KONG FINANCIAL REPORTING STANDARD FORPRIVATE ENTITIES ................................................................................. 4/10 (9/15)HKFRS-PE (Revised) HONG KONG FINANCIAL REPORTING STANDARD FORPRIVATE ENTITIES (REVISED) ........................................................... 5/17 SMALL AND MEDIUM-SIZED ENTITY FINANCIAL REPORTING FRAMEWORKAND FINANCIAL REPORTING STANDARD (SME-FRF & SME-FRS)SME-FRF &SME-FRSSME-FRF & SME-FRF .................................................................................... 8/05 (2/11)SME-FRF &SME-FRS(Revised)SME-FRF & SME-FRF (Revised)..................................................................... 3/14(12/15)ACCOUNTING GUIDELINES (AG)AG 1 Preparation and Presentation of Accounts from Incomplete Records ..............3/84AG 5 Merger Accounting for Common Control Combinations ...................................05/11 (11/13) AG 7 Preparation of Pro Forma Financial Information for Inclusion inInvestment Circulars.....................................................................................3/06ACCOUNTING BULLETINS (AB)AB 3 Guidance on Disclosure of Directors’ Remuneration ........................................1/00AB 4 Guidance on the Determination of Realised Profits and Losses in theContext of Distributions under the Hong Kong Companies Ordinance........5/10AB 5 Guidance for the Preparation of a Business Review under the Hong KongCompanies Ordinance Cap. 622..................................................................7/14AB 6 Guidance on the Requirements of Section 436 of the Hong KongCompanies Ordinance Cap. 622..................................................................6/15。
美国药典热原测试USP151
151 PYROGEN TESTThe pyrogen test is designed to limit to an acceptable level the risks of febrile reaction in the patient to the administration, by injection, of the product concerned. The test involves measuring the rise in temperature of rabbits following the intravenous injection of a test solution and is designed for products that can be tolerated by the test rabbit in a dose not to exceed 10 mL per kg injected intravenously within a period of not more than 10 minutes. For products that require preliminary preparation or are subject to special conditions of administration, follow the additional directions given in the individual monograph or, in the case of antibiotics or biologics, the additional directions given in the federal regulations (see Biologics 1041).APPARATUS AND DILUENTSRender the syringes, needles, and glassware free from pyrogens by heating at 250 for not less than 30 minutes or by any other suitable method. Treat all diluents and solutions for washing and rinsing of devices or parenteral injection assemblies in a manner that will assure that they are sterile and pyrogen-free. Periodically perform control pyrogen tests on representative portions of the diluents and solutions for washing or rinsing of the apparatus. Where Sodium Chloride Injection is specified as a diluent, use Injection containing 0.9 percent of NaCl.TEMPERATURE RECORDINGUse an accurate temperature-sensing device such as a clinical thermometer, or thermistor probes or similar probes that have been calibrated to assure an accuracy of ±0.1 and have been tested to determine that a maximum reading is reached in less than 5 minutes. Insert the temperature-sensing probe into the rectum of the test rabbit to a depth of not less than 7.5 cm, and, after a period of time not less than that previously determined as sufficient, record the rabbit's body temperature.TEST ANIMALSUse healthy, mature rabbits. House the rabbits individually in an area of uniform temperature between 20 and 23 and free from disturbances likely to excite them. The temperature varies not more than ±3 from the selected temperature. Before using a rabbit for the first time in a pyrogen test, condition it not more than seven days before use by a sham test that includes all of the steps as directed for Procedure except injection. Do not use a rabbit for pyrogen testing more frequently than once every 48 hours, nor prior to 2 weeks following a maximum rise of its temperature of 0.6 or more while being subjected to the pyrogen test, or following its having been given a test specimen that was adjudged pyrogenic.PROCEDUREPerform the test in a separate area designated solely for pyrogen testing and under environmental conditions similar to those under which the animals are housed and free from disturbances likely to excite them. Withhold all food from the rabbits used during the period of the test. Access towater is allowed at all times, but may be restricted during the test. If rectal temperature-measuring probes remain inserted throughout the testing period, restrain the rabbits with light-fitting neck stocks that allow the rabbits to assume a natural resting posture. Not more than 30 minutes prior to the injection of the test dose, determine the “control temperature” of each rabbit: this is the base for the determination of any temperature increase resulting from the injection of a test solution. In any one group of test rabbits, use only those rabbits whose control temperatures do not vary by more than 1 from each other, and do not use any rabbit having a temperature exceeding 39.8.Unless otherwise specified in the individual monograph, inject into an ear vein of each of three rabbits 10 mL of the test solution per kg of body weight, completing each injection within 10 minutes after start of administration. The test solution is either the product, constituted if necessary as directed in the labeling, or the material under test treated as directed in the individual monograph and injected in the dose specified therein. For pyrogen testing of devices or injection assemblies, use washings or rinsings of the surfaces that come in contact with the parenterally administered material or with the injection site or internal tissues of the patient. Assure that all test solutions are protected from contamination. Perform the injection after warming the test solution to a temperature of 37 ± 2. Record the temperature at 30-minute intervals between 1 and 3 hours subsequent to the injection.TEST INTERPRETATION AND CONTINUATIONConsider any temperature decreases as zero rise. If no rabbit shows an individual rise in temperature of 0.5 or more above its respective control temperature, the product meets the requirements for the absence of pyrogens. If any rabbit shows an individual temperature rise of 0.5 or more, continue the test using five other rabbits. If not more than three of the eight rabbits show individual rises in temperature of 0.5 or more and if the sum of the eight individual maximum temperature rises does not exceed 3.3, the material under examination meets the requirements for the absence of pyrogens.RADIOACTIVE PHARMACEUTICALSTest Dose for Preformulated, Ready-to-Use Products Labeled with RadioactivityAGGREGATED ALBUMIN and OTHER PARTICLE-CONTAINING PRODUCTSFor the rabbit pyrogen test, dilute the product with Sodium Chloride Injection to not less than 100 µCi per mL, and inject a dose of 3 mL per kg of body weight into each rabbit.OTHER PRODUCTSWhere Physical Half-life of Radionuclide Is Greater Than 1 Day— Calculate the maximum volume of the product that might be injected into a human subject. This calculation takes into account the maximum recommended radioactive dose of the product, in µCi, and the radioactive assay, in µCi per mL, of the product at its expiration date or time. Using this information, calculate the maximum volume dose per kg to a 70-kg human subject.For the rabbit pyrogen test, inject a minimum of 10 times this dose per kg of body weight into eachrabbit. If necessary, dilute with Sodium Chloride Injection. The total injected volume per rabbit is not less than 1 mL and not more than 10 mL of solution.Where Physical Half-life of Radionuclide is Less Than 1 Day— For products labeled with radionuclides having a half-life of less than 1 day, the dosage calculations are identical to those described in the first paragraph under Other Products. These products may be released fordistribution prior to completion of the rabbit pyrogen test, but such test shall be initiated at not more than 36 hours after release.Test Dose for Pharmaceutical Constituents or Reagents to Be LabeledThe following test dose requirements pertain to reagents that are to be labeled or constituted prior to use by the direct addition of radioactive solutions such as Sodium Pertechnetate Tc 99m Injection, i.e., “cold kits”.Assume that the entire contents of the vial of nonradioactive reagent will be injected into a 70-kg human subject, or that 1/70 of the total contents per kg will be injected. If the contents are dry, constitute with a measured volume of Sodium Chloride Injection.For the rabbit pyrogen test, inject (1/7) of the vial contents per kg of body weight into each rabbit. The maximum dose per rabbit is the entire contents of a single vial. The total injected volume per rabbit is not less than 1 mL and not more than 10 mL of solution.Auxiliary Information— Please check for your question in the FAQs before contacting USP. USP34–NF29 Page 115 Topic/Question Contact Expert CommitteeGeneral Chapter Radhakrishna S Tirumalai, Ph.D. Principal Scientific Liaison1-301-816-8339(GCM2010) General Chapters - Microbiology。
SMIT快捷命令总结
Application --------------------------------------------Fast Path -------------------------------------------------------------------------Software Installation and Maintenance -------------------installSoftware License Management -----------------------------licensesDevices -------------------------------------------------dev System Storage Management (Physical & Logical Storage) --storageSecurity and Users --------------------------------------security Communications Applications and Services ----------------commoPrint Spooling ------------------------------------------spooler Problem Determination -----------------------------------problemPerformance and Resource Scheduling----------------------performanceSystem Environments -------------------------------------systemProcesses and Subsystems --------------------------------src Software Installation and Maintenance-------------------------------------Fast path to menu: installTask -------------------------------------------------Fast Path-------------------------------------------------------------------------Install and Update Software ---------------------------install_updateInstall Software --------------------------------------install_latestUpdate Installed Software to Latest Level (Update All) update_allInstall Software Bundle -------------------------------install_bundleUpdate Software by Fix (APAR) -------------------------update_by_fixInstall and Update from ALL Available Software --------install_allList Software and Related Information -----------------list_softwareList Installed Software and Related Information -------list_installedList Installed Software -------------------------------list_installed_swList Applied but Not Committed Software Updates -------list_applied_swShow Software Installation History --------------------show_historyShow Fix (APAR) Installation Status -------------------show_apar_statList Fileset Requisites -------------------------------list_requisitesList Fileset Dependents -------------------------------list_dependentsList Files Included in a Fileset ----------------------list_files List Fileset Containing File --------------------------what_filesetShow Installed License Agreements ---------------------installed_licenseList Software on Media and Related Information --------list_mediaList Filesets in a Bundle -----------------------------list_bundle List Software on Installation Media -------------------list_media_swList Software Fixes (APARs) on Installation Media -----list_media_fixesList Supplemental Fileset Information on Installation Media ------------------------------------------------------------------list_media_infoShow License Agreements on Installation Media --------license_on_mediaSoftware Maintenance and Utilities ----- ----- ----- --maintain_softwareCommit Applied Software Updates(Remove Saved Files) - -commitReject Applied Software Updates(Use Previous Version) -rejectRemove Installed Software ----- ----- ----- ----- ---- remove Copy Software to Hard Disk for Future Installation ---bffcreateCheck Software File Sizes After Installation ----- ----check_filesVerify Software Installation and Requisites ----- -----verify_installSystem Backup Manager ----- ----- ----- ----- ----- ---backsysBack Up the System ----- ----- ----- ----- ----- -----sysbackupBack Up This System to Tape/File ----- ----- ----- ----mksysb Create a Generic Backup CD ----- ----- ----- ----- ---mkcdgenericList Files in a System Image ----- ----- ----- ----- - lsmksysb Restore Files in a System Image ----- ----- ----- -----restmksysbSoftware License Management---------------------------Fast path to menu: licensesTask ----- ----- ----- ----- ----- ----- --- Fast Path-------------------------------------------------------------------------Manage Nodelocked Licenses ----- ----- --- -- --manage_nodelockedAdd Nodelocked License from a File ----- - -- -add_nodelocked_from_fileAdd Nodelocked License from the Keyboard - -add_nodelocked_from_keyboardDelete a Nodelocked License ----- ----- - --- --delete_nodelockedManage License Servers and License Databases --manage_serversShow Server Characteristics ----- ----- -----show_server_characteristicsManage Concurrent Use and Use Once Licenses ----manage_prod_licensesManage Vendor Information in License Databases -manage_vendorsShow License Usage on Servers -- -- -- -- -- --show_server_statusShow License Usage Summary -- -- -- -- -- -- --show_total_license_usageShow Licenses Currently Being Used -- -- -- --show_current_license_usageShow License Information by Server -- -- -- -- -show_installed_licensesShow Licenses Held by a Specific User -- -- -- -show_user_license_heldShow License Agreements -- -- -- -- -- -- -- --show_license_agreeShow Installed License Agreements -- -- -- -- -installed_licenseShow License Agreements on Installation Media -license_on_mediaDevices-------Fast path to menu: devTask-- -- -- -- -- -- -- -- -- -- -- -- -- -- Fast Path-------------------------------------------------------------------------Install/Configure Devices Added After IPL -- -cfgmgr Printer/Plotter -- -- -- -- -- -- -- -- -- -- printerTTY -- -- -- -- -- -- -- -- -- -- -- -- -- -- ttyPTY -- -- -- -- -- -- -- -- -- -- -- -- -- -- ptyConsole -- -- -- -- -- -- -- -- -- -- -- -- --consoleFixed Disk -- -- -- -- -- -- -- -- -- -- -- --diskCD ROM Drive -- -- -- -- -- -- -- -- -- -- -- cdromRead/Write Optical Drive -- -- -- -- -- -- -- rwoptDiskette Drive -- -- -- -- -- -- -- -- -- -- -disketteTape Drive -- -- -- -- -- -- -- -- -- -- -- --tape Communication -- -- -- -- -- -- -- -- -- -- --commodev Graphic Displays -- -- -- -- -- -- -- -- -- --g_displayGraphic Input Devices -- -- -- -- -- -- -- -- inputLow Function Terminal (LFT) -- -- -- -- -- -- lftSCSI Initiator Device -- -- -- -- -- -- -- -- scsiidSCSI Adapter -- -- -- -- -- -- -- -- -- -- -- scsia Asynchronous I/O -- -- -- -- -- -- -- -- -- --aioMultimedia -- -- -- -- -- -- -- -- -- -- -- --mmList Devices -- -- -- -- -- -- -- -- -- -- -- lsattrConfigure/Unconfigure Devices -- -- -- -- -- -devcfg Unconfigure a Device -- -- -- -- -- -- -- -- -devcfg_ucfg Configure a Defined Device -- -- -- -- -- -- -devcfg_cfgInstall Additional Device Software -- -- -- --devinstPCI Hot Plug Manager -- -- -- -- -- -- -- -- -devdrpci Unconfigure a Device -- -- -- -- -- -- -- -- -rmdevConfigure a Defined Device -- -- -- -- -- -- -mkdevInstall/Configure Devices Added After IPL -- -cfgmgrISA Adapters devisaSystem Storage Management-------------------------Fast path to menu: storageTask -- -- -- -- -- -- -- -- -- -- -- -- -- Fast Path-------------------------------------------------------------------------Logical Volume Manager -- -- -- -- -- -- -- lvmVolume Groups -- -- -- -- -- -- -- -- -- -- vgList All Volume Groups -- -- -- -- -- -- -- lsvg2Add a Volume Groups -- -- -- -- -- -- -- -- mkvgSet Characteristics of a Volume Group -- -- vgscList Contents of a Volume Group -- -- -- -- lsvg1Remove a Volume Group -- -- -- -- -- -- -- -reducevg2 Activate a Volume Group -- -- -- -- -- -- --varyonvg Deactivate a Volume Group -- -- -- -- -- -- varyoffvgImport a Volume Group -- -- -- -- -- -- -- -importvgExport a Volume Group -- -- -- -- -- -- -- -exportvgMirror a Volume Group -- -- -- - -- -- -- - mirrorvgUnmirror a Volume Group -- -- -- - -- -- -- unmirrorvg Synchronize LVM Mirrors -- -- -- - -- -- -- syncvgBack Up a Volume Group -- -- -- - -- -- -- -vgbackup Remake a Volume Group -- -- -- - -- -- -- - restvgList Files in a Volume Group Backup -- -- --lsbackvgRestore Files in a Volume Group Backup -- --restsavevg Logical Volumes -- -- -- - -- -- -- - -- -- lvList All Logical Volumes by Volume Group -- lsvgAdd a Logical Volume -- -- -- - -- -- -- - -mklvSet Characteristics of a Logical Volume -- -lvscShow Characteristics of a Logical Volume -- lslvRemove a Logical Volume -- -- -- - -- -- -- rmlvCopy a Logical Volume -- -- -- - -- -- -- - cplvPhysical Volumes -- -- -- - -- -- -- - -- -- -pvAdd a Disk -- -- -- - -- -- -- - -- -- -- - --makdskChange Characteristics of a Physical Volume --chpvList Contents of a Physical Volume -- -- -- --lspvMove Contents of a Physical Volume -- -- -- - migratepv Paging Space pgspAdd Another Paging Space -- -- -- - -- -- -- --mkps Change/Show Characteristics of a Paging Space -chps Remove a Paging Space -- -- -- - -- -- -- - -- rmps Activate a Paging Space -- -- -- - -- -- -- - -swapon Deactivate a Paging Space -- -- -- - -- -- -- -swapoffFile Systems -- -- -- - -- -- -- - -- -- -- -fsList All File Systems -- -- -- - -- -- -- - -lsfsList All Mounted File Systems -- -- -- - -- -mountAdd/Change/Show/Delete File Systems -- -- -- manfs Mount a File System -- -- -- - -- -- -- - -- mountfsMount a Group of File Systems -- -- -- - -- -mountg Unmount a File System -- -- -- - -- -- -- - -umountfs Unmount a Group of File Systems -- -- -- - --umountg Verify a File System -- -- -- - -- -- -- - - fsckBackup a File System -- -- -- - -- -- -- - --backfilesys Restore a File System -- -- -- - -- -- -- - -restfilesysList Contents of a Backup -- -- -- - -- -- --listtocFiles & Directories -- -- -- - -- -- -- - -- filemgrBackup a File or Directory -- -- -- - -- -- -backfileRestore a File or Directory -- -- -- - -- -- restfileList Contents of a Backup -- -- -- - -- -- --listtoc Removable Disk Management -- -- -- - -- -- --rdsList All Mounted File Systems on a Disk -- --lsmntdsk Unmount File Systems on a Disk -- -- -- - -- umntdsk Remove a Disk from the Operating System -- --removedsk Remove a Disk -- -- -- - -- -- -- - -- -- -- rmvdsk1Open Door -- -- -- - -- -- -- - -- -- -- - --open_doorSystem Backup Manager -- -- -- - -- -- -- - -backsysBack Up the System -- -- -- - -- -- -- - -- -sysbackupList Files in a System Image -- -- -- - -- --lsmksysbRestore Files in a System Image -- -- -- - --restmksysb Security & Users----------------Fast path to menu: securityTask -- -- -- - -- -- -- - -- -- -- - -- Fast Path-------------------------------------------------------------------------Users -- -- -- - -- -- -- - -- -- -- -- -usersAdd a User -- -- -- - -- -- -- - -- - -- mkuserChange a User-s Password -- -- -- - - -- passwdChange/Show Characteristics of a User -- chuserLock/Unlock a User-s Account -- -- -- - -lockuserReset User-s Failed Login Count -- -- -- failed_logins Remove a Use -- -- -- - -- -- -- - -- -- rrmuserList All Users -- -- -- - -- -- -- - -- -lsuserGroups -- -- -- - -- -- -- - -- -- -- - -groupsList All Groups -- -- -- - -- -- -- - -- lsgroupAdd a Group -- -- -- - -- -- -- - -- -- -mkgroupChange/Show Characteristics of a Group --chgroupRemove a Group -- -- -- - -- -- -- - -- -rmgroupPasswords -- -- -- - -- -- -- - -- -- -- -- passwordsChange a User-s Password -- -- -- - -- -- --passwdChange/Show Password Attributes for a User -passwdattrs Login Controls -- -- -- - -- -- -- - -- - loginsChange/Show Login Attributes for a User --login_user Change/Show Login Attributes for a Port --login_portRoles -- -- -- - -- -- -- - -- -- -- - -- rolesAdd a Role -- -- -- - -- -- -- - -- -- -- mkroleChange/Show Characteristics of a Role -- -chroleRemove a Role -- -- -- - -- -- -- - -- -- rmroleList All Roles -- -- -- - -- -- -- - -- --lsroleCommunications Applications and Services----------------------------------------Fast path to menu: commoTask -- -- -- - -- -- -- - -- -- -- - -- -- --Fast Path-------------------------------------------------------------------------TCP/IP -- -- -- - -- -- -- - -- -- -- -- -- ---tcpipMinimum Configuration & Startup -- -- -- -- ---mktcpip Further Configuration -- -- -- - -- - -- -- ---configtcp Hostname -- -- -- - -- -- -- - -- -- -- -- -- -hostnameStatic Routes -- -- -- - -- -- -- - - -- -- ---routeNetwork Interfaces -- -- -- - -- -- - -- -- - -netinterfaceName Resolution -- -- -- - -- -- -- - -- -- ---namerslvClient Network Services -- -- -- - -- -- -- ---clientnetServer Network Services -- -- -- - -- -- -- -- ruserManage Print Server -- -- -- - -- -- -- -- -- -serverSelect BSD style rc Configuration -- -- -- -- -setbootup_optionAuthentication Configuration -- -- -- -- -- -- auth_configUse DHCP for TCPIP Configuration & Startup -- -usedhcpIPV6 Configuration -- -- -- - -- -- -- - -- -- configtcp6IPV6 Static Routes -- -- -- - -- -- -- - -- -- route6IPV6 Network Interfaces -- -- -- - -- -- -- - -inet6IPV6 Daemon/Process Configuration -- -- -- - --daemon6 Quality of Service Configuration & Startup -- -configqosStart Using the QoS Subsystem -- -- -- - -- -- startqosStop Using the QoS Subsystem -- -- -- - -- -- -stopqosNFS -- -- -- - -- -- -- - -- -- -- - -- -- -- -nfs_menus Configure TCP/IP (If Not Already Configured) --tcpipNetwork File System (NFS) -- -- -- - -- -- -- -nfsConfigure NFS on This System -- -- -- - -- -- -nfsconfigure Add a Directory to Exports List -- -- -- - -- -mknfsexp Change/Show Attributes of an Exported Directory -- chnfsexp Remove a Directory from Exports List -- -- -- -rmnfsexpAdd a File System for Mounting -- -- -- - -- --mknfsmnt Change/Show Attributes of an NFS File System --chnfsmnt Remove Remove an NFS File System -- -- -- - -- rmnfsmnt Print Spooling--------------Fast path to menu: spoolerTask -- -- -- - -- -- -- - -- -- -- - -- -- --- - - - Fast Path-------------------------------------------------------------------------Start a Print Job -- -- -- - -- -- -- - -- ---- - - - qprtManage Print Jobs - -- - -- -- -- - -- -- - --- - - - jobsCancel a Print Job - -- - -- -- -- - -- -- - -- - - - qcanShow the Status of Print Jobs - -- - -- -- - -- - - - qchk Prioritize a Print Job - -- - -- -- -- - --- -- - - - qpriHold/Release a Print Job - -- - -- -- -- - ---- - - - qhldMove a Job Between Print Queues - -- - -- -- -- - - - qmove Manage Print Queues - -- - -- -- -- - -- -- --- - - - pqmanage Show Status of Print Queues - -- - -- -- -- --- - - - qstatus Stop a Print Queue - -- - -- -- -- - -- -- - -- - - - qstopStart a Print Queue - -- - -- -- -- - -- -- --- - - - qstartSet the System-s Default Print Queue - -- - --- - - - qdefault Add a Print Queue - -- - -- -- -- - -- -- -- -- - - - mkpqAdd an Additional Printer to an Existing Print Queue -mkqprtChange/Show Print Queue Characteristics -- - - - -- - chpq Remove a Print Queue -- - - - -- - - - -- - - - -- - -rmpq Manage Print Server server Programming Tools -- - - -pqtoolsProblem Determination---------------------Fast path to menu: problemTask -- - - - -- - - - -- - - - -- - - - -- - - - Fast Path-------------------------------------------------------------------------Error Log -- - - - -- - - - -- - - - -- - - - -- - -- - error Generate Error Report -- - - - -- - - - -- - - - -- --- errpt Change/Show Characteristics of the Error Log -- - - - --errdemonClean the Error Log -- - - - -- - - - -- - - - -- - - - errclear System Dump -- - - - -- - - - -- - - - -- - - - -- - - -dump Change the Primary Dump Device -- - - - -- - - - -- - -dumpchgpChange the Secondary Dump Device -- - - - -- - - - -- -dumpchgsChange the Directory to which Dump is Copied on Boot --dumpchgdCopy a System Dump from a Dump Device to a File -- - - -dump_copy_fileCopy a System Dump from a Dump Device to Diskette -- - -dump_copy_dsktAlways Allow System Dump -- - - - -- - - - -- - - - -- -dump_allowSystem Dump Compression -- - - - -- - - - -- - - - -- -dump_comprsCheck Dump Resources Utility -- - - - -- - - - -- - - -dump_checkrAlog -- - - - -- - - - -- - - - -- - - - -- - - - -- - -alogShow an Alog file -- - - - -- - - - -- - - - -- - - - --alog_show Change/Show Characteristics of an Alog File -- - - - --alog_changeHardware Diagnostics -- - - - -- - - - -- - - - -- - - -diagVerify Software Installation and Requisites -- - - - --verify_installPerformance & Resource Scheduling---------------------------------Fast path to menu: performanceTask -- - - - -- - - - -- - - - -- - - - -- - Fast Path-------------------------------------------------------------------------Resource Status & Monitors -- - - - -- - - - -monitorsAnalysis Tools -- - - - -- - - - -- - - - -- -analysisResource Controls -- - - - -- - - - -- - - - -controlsRemove a Process killSet Initial Priority of a Process -- - - - -- niceChange Initial Priority of a Process -- - - - reniceSet System Run Level -- - - - -- - - - -- - - telinitSchedule Jobs -- - - - -- - - - -- - - - -- - atPower Management -- - - - -- - - - -- - - - --pmConfigure/Unconfigure Power Management -- - - pmConfig System State Transition from Enable State -- -pmState Display Power Management -- - - - -- - - - -- pmDisplaySelect Battery -- - - - -- - - - -- - - - -- - - - --pmBatteryWorkload Management -- - - - -- - - - -- - - -wlmWork on alternate configurations - - - - - - -wlmconfigCopy a configuration - - - - - - - - - - - - -wlmconfig_copy Create a configuration - - - - - - - - - - - -wlmconfig_create Select a configuration - - - - - - - - - - - -wlmconfig_selectEnter configuration description - - - - - - - wlmconfig_enter Remove a configuration - - - - - - - - - - - -wlmconfig_delete Work on a set of Subclasses - - - - - - - - - wlmsubclassAdd a class - - - - - - - - - - - - - - - - - wlmaddclassChange/Show Characteristics of a class - - - -wlmchclass General characteristics of a class - - - - - -wlmclass_galCPU resource management - - - - - - - - - - - wlmclass_cpu Memory resource management - - - - - - - - - -wlmclass_memdiskIO resource management - - - - - - - - - -wlmclass_bio Remove a class - - - - - - - - - - - - - - - -wlmrclassClass assignment rules - - - - - - - - - - - -wlmrsCreate a new Rule - - - - - - - - - - - - - - crewlmrsChange/Show Characteristics of a Rule - - - - chgwlmrsStart/Stop/Update WLM - - - - - - - - - - - - wlmmanageStart Workload Managementwlmmanage - - - - - -wlmstart Update Workload Management - - - - - - - - - -wlmupdate Stop Workload Management - - - - - - - - - - -wlmoffAssign/Unassign processes to a class/subclass - - wlmassign System Environments-------------------Fast path to menu: systemTask - - - - - - - - - - - - - - - - - - - - - - - -Fast Path-------------------------------------------------------------------------Stop the System - - - - - - - - - - - - - - - - - - systemAssign the Console - - - - - - - - - - - - - - - - -chconsChange/Show Date and Time - - - - - - - - - - - - - chtz_date Change/Show Date & Time - - - - - - - - - - - - - - date Change Time Zone Using System Defined Values - - - -chtzChange Time Zone Using User Inputted Values - - - -chtz_userManage Language Environment - - - - - - - - - - - - mlang Change/Show Primary Language Environment - - - - - -chlangAdd Additional Language Environments - - - - - - - -mle_add_langRemove Language Environments - - - - - - - - - - - -mle_rm_lang_hdrChange/Show Language Hierarchy - - - - - - - - - - -mle_hier_cmd_hdrSet User Languages - - - - - - - - - - - - - - - - -chlang_user Change/Show Applications for a Language - - - - - -mle_chapp_menuConvert System Messages and Flat Files - - - - - - -nu_iconv Change/Show Characteristics of Operating System - - chgsys Change/Show Number of Licensed Users - - - - - - - -chlicenseManage AIX Floating User Licenses for this Server -netls_serverBroadcast Message to all Users - - - - - - - - - - -wallManage System Logs - - - - - - - - - - - - - - - - -logsChange/Show Characteristics of System Dump - - - - -dump Internet and Documentation Services - - - - - - -web_configureChange/Show Default Browser - - - - - - - - - - -change_default_browserChange Documentation and Search Server - - - - -change_doc_search_serverChange/Show Default Documentation Language - - -chdoclangWeb-based System Manager - - - - - - - - - - - -web_based_system_managerChange System User Interface - - - - - - - - - - dt_config Change/Show Default Documentation Language - - -chdoclangManage Remote Reboot Facility - - - - - - - - - -rrbttyManage System Hang Detection - - - - - - - - - - shd Processes & Subsystems----------------------Fast path to menu: srcTask - - - - - - - - - - - - - - - - -Fast Path-------------------------------------------------------------------------Processes - - - - - - - - - - - - - - -processRemove a Process - - - - - - - - - - - killBind a Process to a Processor - - - - -bindprocUnbind a Process - - - - - - - - - - - unbindprocSubsystems - - - - - - - - - - - - - - subsysQuery a Subsystem - - - - - - - - - - -qssysStart a Subsystem - - - - - - - - - - -startssysStop a Subsystem - - - - - - - - - - - stopssysStop a Single Subsystem - - - - - - - -stopassysStop All Subsystems - - - - - - - - - -stopallssysRefresh a Subsystem - - - - - - - - - -refreshTrace Subsystem - - - - - - - - - - - -tracessysStart Trace - - - - - - - - - - - - - -tracessysonStop Trace - - - - - - - - - - - - - - tracessysoffSubservers - - - - - - - - - - - - - - subserverQuery a Subserver - - - - - - - - - - -qserverStart a Subserver - - - - - - - - - - -startserverStop a Subserver - - - - - - - - - - - stopserverTrace Subserver - - - - - - - - - - - -traceserverStart Trace - - - - - - - - - - - - - -startserver.traceStop Trace - - - - - - - - - - - - - - stopserver.trace。
Stata 19 综述及命令概述说明书
19Immediate commandsContents19.1Overview19.1.1Examples19.1.2A list of the immediate commands19.2The display command19.3The power command19.1OverviewAn immediate command is a command that obtains data not from the data stored in memory but from numbers typed as arguments.Immediate commands,in effect,turn Stata into a glorified hand calculator.There are many instances when you may not have the data,but you do know something about the data,and what you know is adequate to perform statistical tests.For instance,you do not have to have individual-level data to obtain the standard error of the mean,and thereby a confidence interval, if you know the mean,standard deviation,and number of observations.In other instances,you may actually have the data,and you could enter the data and perform the test,but it would be easier if you could just ask for the statistic based on a summary.For instance,youflip a coin10times,and it comes up heads twice.You could enter a10-observation dataset with two ones(standing for heads) and eight zeros(meaning tails).Immediate commands are meant to solve those problems.Immediate commands have the following properties:1.They never disturb the data in memory.You can perform an immediate calculation as an asidewithout changing your data.2.The syntax for these commands is the same,the command name followed by numbers,whichare the summary statistics from which the statistic is calculated.The numbers are almost always summary statistics,and the order in which they are specified is in some sense“natural”.3.Immediate commands all end in the letter i,although the converse is not ually,if thereis an immediate command,there is a nonimmediate form also,that is,a form that works on the data in memory.For every statistical command in Stata,we have included an immediate form if it is reasonable to assume that you might know the requisite summary statistics without having the underlying data and if typing those statistics is not absurdly burdensome.4.Immediate commands are documented along with their nonimmediate counterparts.Thus,if youwant to obtain a confidence interval,whether it be from summary data with an immediate command or using the data in memory,use the table of contents or index to discover that[R]ci discusses confidence intervals.There,you learn that ci calculates confidence intervals by using the data in memory and that cii does the same with the data specified immediately following the command.12[U]19Immediate commands19.1.1ExamplesExample1Let’s take the example of confidence intervals.Professional papers often publish the mean,standard deviation,and number of observations for variables used in the analysis.Those statistics are sufficient for calculating a confidence interval.If we know that the mean mileage rating of cars in some sample is24,that the standard deviation is6,and that there are97cars in the sample,we can calculate .cii97246Variable Obs Mean Std.Err.[95%Conf.Interval]9724.609207722.7907325.20927We learn that the mean’s standard error is0.61and its95%confidence interval is[22.8,25.2].To obtain this,we typed cii(the immediate form of the ci command)followed by the number of observations,the mean,and the standard deviation.We knew the order in which to specify the numbers because we had read[R]ci.We could use the immediate form of the ttest command to test the hypothesis that the true mean is22:.ttesti9724622One-sample t testObs Mean Std.Err.Std.Dev.[95%Conf.Interval]x9724.6092077622.7907325.20927 mean=mean(x)t= 3.2830 Ho:mean=22degrees of freedom=96 Ha:mean<22Ha:mean!=22Ha:mean>22 Pr(T<t)=0.9993Pr(|T|>|t|)=0.0014Pr(T>t)=0.0007Thefirst three numbers were as we specified in the cii command.ttesti requires a fourth number, which is the constant against which the mean is being tested;see[R]ttest.Example2We mentionedflipping a coin10times and having it come up heads twice.The99%confidence interval can also be obtained from ci:.cii102,level(99)Binomial ExactVariable Obs Mean Std.Err.[99%Conf.Interval]10.2.1264911.0108505.6482012In the previous example,we specified cii with three numbers following it;in this example,we specify2.Immediate commands often determine what to do by the number of arguments following the command.With two arguments,ci assumes that we are specifying the number of trials and successes from a binomial experiment;see[R]ci.[U]19Immediate commands3The immediate form of the bitest command performs exact hypothesis testing:.bitesti102.5N Observed k Expected k Assumed p Observed p10250.500000.20000Pr(k>=2)=0.989258(one-sided test)Pr(k<=2)=0.054688(one-sided test)Pr(k<=2or k>=8)=0.109375(two-sided test)For a full explanation of this output,see[R]bitest.Example3Stata’s tabulate command makes tables and calculates various measures of association.The immediate form,tabi,does the same,but we specify the contents of the table following the command:.tabi510\214colrow12Total151015221416Total72431Fisher’s exact=0.2201-sided Fisher’s exact=0.170The tabi command is slightly different from most immediate commands because it uses‘\’to indicate where one row ends and another begins.4[U]19Immediate commands19.1.2A list of the immediate commandsCommand Reference Descriptionbitesti[R]bitest Binomial probability testcci[ST]epitab Tables for epidemiologistscsiirimccicii[R]ci Confidence intervals for means,proportions,and counts esizei[R]esize Effect size based on mean comparisonprtesti[R]prtest Tests of proportionssdtesti[R]sdtest Variance comparison testssymmi[R]symmetry Symmetry and marginal homogeneity teststabi[R]tabulate twoway Two-way tables of frequenciesttesti[R]ttest Mean comparison teststwoway pci[G-2]graph twoway pci Paired-coordinate plot with spikes or linestwoway pcarrowi[G-2]graph twoway pcarrowi Paired-coordinate plot with arrowstwoway scatteri[G-2]graph twoway scatteri Twoway scatterplot19.2The display commanddisplay is not really an immediate command,but it can be used as a hand calculator..display2+57.display sqrt(2+sqrt(3^2-4*2*-2))/(2*3).44095855See[R]display.19.3The power commandpower is not technically an immediate command because it does not do something on typed numbers that another command does on the dataset.It does,however,work strictly on numbers you type on the command line and does not disturb the data in memory.power performs power and sample-size analysis.See Stata Power and Sample-Size Reference Manual.。
英文净含量的标准表示
英文净含量可以使用以下标准表示：
1."Net weight"：用于表示产品的净重，通常用于固体和液体商品，如食品、化
妆品、清洁剂等。
2."Net contents"：用于表示产品的净含量，主要用于液体、散装或非固定形状的
商品，如果汁、药剂、颗粒状产品等。
3."Net volume"：用于表示产品的净体积，通常用于液体和气体产品，如饮料、
气体罐装产品等。
这些标准表示方式通常会紧跟在产品名称或描述后面，并以统一的单位（如
g、ml、oz等）进行补充。
例如，一个食品包装上可以标注为"Net weight:
200g"，一个饮料瓶上可以标注为"Net volume: 500ml"。
需要注意的是，英文净含量标准表示可能会根据不同的国家和地区而有所不同。
因此，在进行国际贸易或销售时，最好遵守当地法规和规定，以确保产品标签的合规性和正确性。
用于Illumina MiSeq 系统的Embgenix PGT-A试剂盒（RUO）Protocol
I. IntroductionThe Embgenix™ PGT-A Kit (RUO) (Cat. No. 634760) has been developed to analyze DNA obtained frombiopsies of single or multiple embryonic cells for preimplantation genetic testing for aneuploidies (PGT-A).NOTE: This document provides brief instructions for the protocol steps and should only be used by personnel who are experienced with performing this experiment. The Embgenix PGT-A (RUO) User Manual for Illumina® MiSeq System should be referred to by newer users or for additional protocol details.II. General ConsiderationsRefer to the user manual for more detailed information about the points below.•Requirements for Preventing Contamination: Make sure you have two physically separated workstations.Refer to the user manual for more detailed information.•Sample Recommendations and Requirements: Cells from trophectoderm (TE) biopsies and low amounts of Genomic DNA (gDNA).•Positive and Negative Controls: Use 30 pg of freshly diluted reference gDNA as a positive control by adding 2.5 µl of a 12 pg/μl dilution. Use 2.5 µl of 1X PBS (Ca2+- and Mg2+-free) as a negative control.•During the protocol, thaw components on ice and briefly mix and quick-spin component tubes before use. III. ProtocolA. Cell Lysis/gDNA Extraction1.Thaw the Cell Extraction Buffer, WGA Buffer, and WGA Nuclease-Free Water on ice.2.Calculate and prepare the Cell Extraction Master Mix (CEMM) on ice as described below for allreactions, plus 10% extra. Mix thoroughly with a pipette and then spin down to collect the contents atthe bottom of the tube.4.8 µl Cell Extraction Buffer0.6 µl Cell Extraction Enzyme22.1µl WGA Nuclease-Free Water27.5 µl Total volume per reactionNOTE: The CEMM volume should be 27.5 μl for each 2.5 µl of sample in a final reaction volume of30 µl (Step 3). If the sample volume is larger than 2.5 µl, refer to the user manual.3.To each sample or control, add [30.0 μl – Sample Volume] of CEMM from Step 2.4.Spin briefly to collect the contents at the bottom of the tubes.5.Place the samples in a thermal cycler with a heated lid set to 100°C–105°C. Perform the Cell LysisReaction using the following program:75°C 10 min95°C 4 min4°C Hold6.Once the thermal cycler reaches 4°C, spin briefly to collect the contents at the bottom of the tubes andimmediately proceed to the next section.B. Whole Genome Amplification1.On ice, assemble the WGA Master Mix for all reactions, plus 10% extra. Mix thoroughly by gentlevortexing for 5 sec, and then spin briefly to collect the contents at the bottom of the tubes.29.8 µl WGA Buffer1.0 µl WGA Enzyme14.2 µl WGA Nuclease-Free Water45.0 µl Total Volume per reaction2.Add 45 μl of WGA Master Mix to each well/tube of the Cell Lysis reaction (Section III.A, Step 6).Mix by gentle vortexing for 5 sec and then spin briefly to collect the contents at the bottom of thetubes.3.Return the tubes to the thermal cycler with the heated lid set to 101°C–105°C. Perform the WGAReaction using the following cycling conditions:95°C 2 min12 cycles:95°C 15 sec15°C 50 sec25°C 40 sec35°C 30 sec65°C 40 sec75°C 40 sec14 cycles:95°C 15 sec65°C 1 min75°C 1 min75°C 5 min4°C Hold4.At the end of the amplification, spin briefly to collect the contents at the bottom of the tubes.SAFE STOPPING POINT: The samples can be stored in the thermal cycler at 4°C overnight ortransferred to –20°C for up to a week.C. Dilution of Whole-Genome-Amplified ProductsDilution of the WGA product 1:20 (WD1)1.Pipette 76 µl of Embgenix WGA Dilution Solution to each well or tube labeled WD1.2.To this, add 4 µl of the WGA product (from previous section) from samples, positive control, or no-template control.3.Seal the plate or close the tubes, mix by gentle vortexing and spin briefly to collect the contents at thebottom of the well or tubes.Dilution of the WGA product 1:240 (WD2)4.Pipette 55 µl of Embgenix WGA Dilution Solution to each well or tube labeled WD2.5.To this, add 5 µl of the contents of WD1 from Step 3.6.Seal the plate or close the tubes, mix by gentle vortexing, and spin briefly to collect the contents at thebottom of the well or tube.D. Determination of WGA Product Quality and Quantity (Optional)Quantification of WGA product (Optional):•WGA products can be quantified using a fluorescence-detection-based method such as Qubit dsDNA HS Assay on the Qubit Fluorometer.See Qubit dsDNA HS Assay user manual for detailedinstructions.•Use 10 µl of the WD1 from Section III.C, Step 3 for sample measurement.The concentration of the WGA product or positive control is determined by multiplying the sampleconcentration measurement with the dilution factor 20. The result is usually greater than 24 ng/µl.Determination of WGA Quality and Size (Optional): The quality and size of the WGA product can be assessed using either the 4200 TapeStation or the 2100 BioAnalyzer from Agilent.•Use 2 µl of WD1 from Section III.C, Step 3 for size assessment using the 4200 TapeStation with High Sensitivity D5000 Reagents from Agilent.•Alternatively, you can use 1 µl of WD1 for validation using the Agilent 2100 Bioanalyzer with Agilent’s High Sensitivity DNA Kit.The expected average size of the WGA products or positive control should range between 500–900 bp.E. Library Preparation: Fragmentation and Adapter Ligation1.Thaw the following items from Embgenix Library Preparation Reagents (Box 2) on ice: FE DilutionBuffer, Lib Prep Buffer, Rxn Enhancer, and Stem-Loop Adapters.NOTE:Keep the 10X FE and the Library Prep Enzyme in the –20°C freezer until needed. Use abenchtop cooler to keep the enzymes cold while working with them at the bench.2.Once thawed, gently vortex each of the components and then spin down the tubes briefly to collectthe contents at the bottom of the tubes. Keep on ice.3.On ice, add 4 µl of Stem-Loop Adapters to each tube/well of PCR tubes, 8-tube PCR strips, or a 96-well PCR plate, according to the number of reactions to be performed.4.Add 8 µl of freshly diluted WD2 (1:240 dilution) from Section III.C, Step 6 or 8 µl WGA DilutionSolution as a negative control to a tube/well containing the 4 µl of Stem-Loop Adapters.5.On ice, prepare 1X FE by diluting the 10X FE in thawed but cold FE Dilution Buffer in a 1:9 ratio (1part 10X FE to 9 parts FE Dilution Buffer).•Prepare enough material to accommodate the Library Prep Master Mix in Step 6, plus 10% of the total reaction mix volume.• A minimum of 40 µl of 1X FE Preparation should be assembled.•Mix gently by pipetting up and down 10 times.•Spin down and keep on ice.Component 1 rxn 1–36 rxns*FE Dilution Buffer 0.9 µl 36.0 µl 10X FE 0.1 µl 4.0 µlTotal volume (1X FE) 1.0 µl 40.0 µl *Volumes in µl include 10% overageAfter preparing, immediately proceed to the next step.6.Calculate and prepare the Library Prep Master Mix on ice for all reactions plus 10% of the totalreaction mix volume. Combine the following reagents in the order shown:4.0 µl Lib Prep Buffer3.5 µl Rxn Enhancer2.0 µl Lib Prep Enzyme1.0 µl 1X FE (from Step 5)10.5 µl Total volume per reaction7.Assemble the library preparation reaction on ice. To each tube/well from Step 4, add 10.5 µl of theLibrary Prep Master Mix prepared in Step 5.8.Mix by gentle vortexing for 5 sec, and then spin the tubes briefly to collect the contents at the bottomof the tubes/wells.Keep the samples cold (4°C) until placed in the thermal cycler.9.Perform the library preparation reaction by placing the tube/plate in a precooled thermal cycler andrunning the following program:20°C 40 min85°C 5 min4°C Hold10.Once the thermal cycler reaches 4°C, spin briefly.SAFE STOPPING POINT: Samples can be stored in the thermal cycler at 4°C overnight or at –20°C for up to a week .F. Library Amplification and Indexing with UDI1.Thaw the library samples and the following items on ice: Unique Dual Index (1–96) andAmplification Buffer. Once thawed, mix each component by gentle vortexing, and then spin down thetubes briefly to collect the contents at the bottom of the tubes. Keep on ice.NOTE: Keep the PrimeSTAR® HS DNA Polymerase (5 U/µl) in the –20°C freezer until needed. Usea benchtop cooler to keep the enzyme cold while working with it on the bench.2.Calculate and prepare on ice the Library Amplification Master Mix for all reactions plus 10% ofthe total reaction mix volume by combining the following reagents:21.5 µl Amplification Buffer3 µl Nuclease-Free Water1.0 µl PrimeSTAR HS DNA Polymerase25.5 µl Total volume per reaction3.Add 25.5 µl of the Library Amplification Master Mix to each reaction from Section III.E, Step 8.4.Add 2 µl of a different index from the Unique Dual Index (1–96) to each reaction. Use a new pipettetip for each UDI.5.Mix by vortexing for 5 sec and then spin briefly to collect the contents at the bottom of thetubes/plate.6.Place in a thermal cycler with a heated lid (105°C) and perform PCR amplification using thefollowing program:72°C 3 min85°C 2 min98°C 2 min15 cycles98°C 20 sec60°C 75 sec68°C 5 min4°C Hold7.Once the thermal cycler reaches 4°C, spin briefly to collect the contents at the bottom of the tubes.SAFE STOPPING POINT: The samples can be stored in the thermal cycler at 4°C overnight or at−20°C for up to a week.G. Purification of Amplified Libraries1.Bring the NucleoMag NGS Clean-up and Size Select beads and the Nuclease-Free Water to roomtemperature for at least 30 min and mix well by vortexing.2.Prepare fresh 80% ethanol. You will need ~400 μl per sample.3.A dd 40 μl of NucleoMag NGS Clean-up and Size Select beads to the amplified libraries fromSection III.B, Step 7. Mix well by vortexing or pipetting the entire mixture up and down 10 times.4.Incubate at room temperature for 5 min to let the libraries bind to the beads.5.Briefly spin the samples to collect the liquid to the bottom of the tubes.6.Place the tubes on a magnetic stand until the liquid appears to be completely clear and there are nobeads left in the supernatant (~2 min).7.Remove and discard the supernatant while samples are on the magnetic stand without disturbing thebeads.8.Keep the samples on the magnetic stand. Add 200 μl of fresh 80% ethanol to each sample withoutdisturbing the beads. Incubate for 30 sec, and then remove and discard the supernatant, taking carenot to disturb the beads.9.Repeat the ethanol wash (Step 8).10.Briefly centrifuge the samples to collect the liquid at the bottom of the tubes.11.Place the samples on the magnetic stand for 30 sec, then remove any residual ethanol with a pipette.12.Incubate the samples at room temperature until the pellet is no longer shiny, but before cracks appear(~5–10 mins). Check the pellet frequently during this time and continue to Step 13 when it is dryenough.13.Once the beads are dry, add 27 μl of Nuclease-Free Water to each sample.14.Remove the samples from the magnetic stand and vortex the tubes for 5–10 sec to resuspend thebeads.15.Incubate at room temperature for ~5 min to rehydrate the beads.16.Briefly spin the samples to collect the liquid from the side of the tubes.17.Place the tubes back on the magnetic stand until the solution is completely clear (~2 min or longer).18.Transfer 25 μl of supernatant containing purified libraries to a nuclease-free, low-adhesion tubetaking care to not touch the magnetic bead pellet. Label each tube with sample information.SAFE STOPPING POINT: The purified libraries can be immediately validated or stored at –20°C.H. Validation and Quantification of Amplified LibrariesQuantification:•Libraries are quantified using fluorescence-detection-based methods such as Qubit 2.0 Fluorometer with the Qubit dsDNA HS Assay kit or Quant-iT dsDNA Assay kit. See assay user manuals for moredetails•Use 2 µl of the purified libraries from Section III.G, Step 18 for the measurement.•The measured concentrations of the libraries or positive control should be greater than 4 ng/µl.Quality and Size Assessment (optional):• A fragment analyzer such as the Agilent 4200 TapeStation or the 2100 Bioanalyzer is used to assess the quality and size of the libraries. For detailed instructions, see the Agilent High Sensitivity D1000ScreenTape System or Agilent High Sensitivity DNA Kit User Manual.•Dilute aliquots of the purified libraries from Section III.G, Step 18 in Nuclease-Free Water to 3 ng/µl.o For validation using the 4200 TapeStation, use 2 µl of these diluted samples.o Alternatively, use 1 µl of these diluted samples for size assessment using the 2100 Bioanalyzer.The libraries should have an average fragment size between 300–600 bp using a region of 150–1,000 bp.I. Library Pooling and Illumina SequencingMultiplexed libraries are sequenced simultaneously in a single sequencing run. The different UDIs allowthe demultiplexing of the sequencing data specific to each sample. Refer to the Embgenix PGT-A Kit(RUO) User Manual for MiSeq System for details.Sample Pooling and loading concentration of pooled libraries:When using the MiSeq Reagent Kit v3, up to 24 libraries can be pooled into a single run. We recommenda loading concentration of 11 pM for the denatured pooled libraries.Contact UsCustomer Service/Ordering Technical Supporttel: 800.662.2566 (toll-free) tel: 800.662.2566 (toll-free)fax: 800.424.1350 (toll-free) fax: 800.424.1350 (toll-free)web: /service web: /supporte-mail: **********************e-mail: *******************************Notice to PurchaserOur products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.Your use of this product is also subject to compliance with any applicable licensing requirements described on the product’s web page at . It is your responsibility to review, understand and adhere to any restrictions imposed by such statements© 2022 Takara Bio Inc. All Rights Reserved.All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at .This document has been reviewed and approved by the Quality Department.。