异莲心碱对D-半乳糖致衰老小鼠抗氧化作用的研究

异莲心碱对D-半乳糖致衰老小鼠抗氧化作用的研究
刘斯灵;郝亚荣;秦健;余莉
【期刊名称】《中国医药导报》
【年(卷),期】2011(8)20
【摘要】Objective: To study the antioxidative effect of isoliensinine in D-Galactose-induced aged mice.Methods: The fifty mouses were randomly divided into five groups involved in black group, model group, low-dose isoliensinine group (10 mg/kg), high-dose isoliensinine group (20 mg/kg) and the group treated with Vitamin C.The body weight and the liver in -dex were determined and the activities of super-oxide dismutase (SOD), glutathione peroxidase (GSH-Px) and the contents of malonic dialdehyde (MDA) in serum and liver tissue were assayed respectively.Results: Compared with black group, for aging model mice, the body weight and the liver index declined, the data were 32.87 g and 3.87 mg/g, the activities of SOD and GSH-Px in surum and liver tissue also declined, the data were 247, 408 U/ml and 358, 319 U/mgpro, and the contents of MDA raised, the data were 20.62 nmol/ml and 13.01 nmol/mgpro (P＜0.01).Dn comparison with the model group, the loss of body weight and the liver index were markedly counteracted, the activities of SOD and GSH-Px in surum and liver tissue of isoliensinine group (20 mg/kg) were all increased and the contents of MIA got high reduction (P＜0.01), the data were 36.29 g,4.53 mg/g, 305, 459 U/ml, 438, 355 U/mgpro and 16.47 nmol/ml
respectively.The activities of GSH-Px in liver tissue was 333 U/mgpro and had no marked effect compared with model group.Conclusion: Isoliensinine can significantly increase the antioxidative effect of D-Galactose-induced aged mice.%目的:探讨异莲心碱对D-半乳糖致衰老小鼠抗
氧化的作用.方法:选取昆明种成年小鼠50只,随机将其分为空白组、模型组、异莲
心碱低剂量组(10 mg/kg)、高剂量组(20 mg/kg)及维生素C(Vitamin C)对照组,
测定小鼠体重和肝脏指数,以小鼠血清和肝脏组织中的超氧化物歧化酶(SOD)、谷
胱甘肽过氧化物酶(GSH-Px)的活性及 MDA 含量为指标,考察异莲心碱在体内的抗氧化性能.结果:与空白组比较,模型组小鼠体重和肝脏指数明显下降,为32.87 g和3.87 mg/g;血清和肝脏组织 SOD和GSH-Px 活性明显降低,其值分别为247 U/ml、408 U/ml和358、319 U/mgpro;MDA含量为20.62 nmol/ml和13.01
nmol/mgpro,明显增加(P＜0.01);与模型组比较,异莲心碱20 mg/kg可明显拮抗
小鼠体重和肝脏指数的降低、增强血清和肝脏组织 SOD和GSH-Px 活性、减少MDA含量(P＜0.01),其值分别为36.29 g,4.53 mg/g,305、459 U/ml,438、355
U/mgpro和16.47 nmol/ml.异莲心碱低剂量组小鼠肝脏组织GSH-Px 活性为
333 U/mgpro,与模型组比较差异无统计学意义.结论:异莲心碱能显著提高D-半乳糖致衰老小鼠的抗氧化能力.
【总页数】3页(P5-6,9)
【作者】刘斯灵;郝亚荣;秦健;余莉
【作者单位】武汉大学人民医院老年病科,湖北武汉,430060;武汉大学人民医院老
年病科,湖北武汉,430060;武汉大学人民医院老年病科,湖北武汉,430060;武汉大学
人民医院老年病科,湖北武汉,430060
【正文语种】中文
【中图分类】R965
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