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ZEISS Axio Imager Light Manager手册说明书
Unlike motorised stands, every value must be saved individually via the LM Set button before an objective, tube lens or reflector ischanged. Light Manager values for transmitted light (TL) and reflected light (RL) are always processed separately.In this case the encoding of objective and reflector turret is recognised (⇒ up to two "Master" components) according to the position ofthe toggle switch RL/TL.1. Toggle switch RL/TL in position TL:Saves the brightness values for the objective turret (max. 7)2. Toggle switch RL/TL in position RL:Saves the brightness values for a matrix from the objective turret (max. 7) x reflector turret (6 or 10) (see table below)Dependent components (if encoded)Type of illuminationObjective turret Reflector turretTransmitted lightX (halogen lamp for transmitted light, toggleswitch RL/TL on TL)Reflected lightX X (halogen lamp for reflected light, toggle switchRL/TL on RL)In this case the tube lens turret is under PC control (⇒ up to three "Master" components).1. Toggle switch RL/TL in position TL:Saves the brightness values for a matrix from the objective turret (max. 7) x tube lens turret (4)(Bertrand lens position is not included: only one brightness can be saved for this position)2. Toggle switch RL/TL in position RL:Saves the brightness values for a matrix from the objective turret (max. 7) x tube lens turret (4) x reflector turret (max.10) (see tablebelow)Dependent components (if encoded))Type of illuminationObjective turret Reflector turret Tube lens turretTransmitted light:X X Toggle switch RL/TL on TLReflected light:X X X Toggle switch RL/TL on RLDefault setting of the stand after switching on:Transmitted light:• Toggle switch RL/TL on TLButton TL on (shutter open or lamp on)Button RL offReflected light:• Toggle switch RL/TL on RLButton TL offButton RL on (shutter open or lamp on)Saving LM value:• To save the current lamp voltage for the current objective turret position press the LM Set button brieflySaving 3200K:This function determines whether the stand is set at 3200K when it is switched on.• To set 3200K to be active on switching on: activate 3200K and press LM Set button.• To set 3200K to be inactive on switching on: deactivate 3200K and press LM Set button.The 3200K setting is saved globally and is independent of other LM values that have already been saved. The normal LM values are available at any time as soon as 3200K is deactivated.Overwriting the LM values:• To save the new value at the relevant position press the LM Set buttonDeleting of the LM values:This is not possible.Activating an LM value:This is done by switching on and changing the position of a "Master" component.To permanently deactivate/activate Light Manager (LM) & antiglare device (AG)• Keep the "RL" button pressed down when you switch on:• One beep signifies deactivation.• Two beeps signify activation.To permanently deactivate/ activate Light Manager only• Keep "3200"button pressed down when you switch on:One beep signifies deactivation. Two beeps signify activation.To permanently deactivate/ activate antiglare device only• Keep “TL” button pressed down when you switch on:One beep signifies deactivation. Two beeps signify activation• If button "RL" is pressed when you switch on and only one of the two functions is activated, that function will be deactivated:Starting condition OutcomeLM AG LM AG⇒0 01 1⇒0 01 0⇒0 00 1⇒ 1 10 0These parameters can also be set via MTB 2004 for motorised stands.Antiglare device:If there is a shutter in the TL optical path, the lamp voltage remains constant when the objective is changed and the shutter takes over thefunction of the antiglare device.If no shutter is present, the lamp is switched off.Safety function:If the reflector turret flap is opened or the reflector turret is completely removed, the safety switch-off device automatically closes thereflected light shutter. In addition, the shutter can no longer be opened by pressing a button as long as the reflected light path is "open".The shutter also closes automatically when the stand is switched off.The brightness of the Light Control LEDs can be adjusted by the user.Manual stands:• Keep SET button pressed down for about 3 seconds until a long beep is heard.All LEDs go on.The brightness of the LEDs can now be adjusted by the brightness control (control knob).However, the brightness cannot be completely extinguished!Activating the control knob in this mode has no effect on the lamp voltage!This mode is exited automatically by releasing the LM Set buttonThe setting is saved permanently!Motorised stands:The adjustment of the LED brightness is linked with the brightness control of the TFT Display.All motorised reflector turrets can be mounted on the stands D1 and D1m. The reflector turrets for the D1 stands have been incorporated into the MTB 2004 in the same way as those for the motorised stands.The motorised reflector turrets can be operated either by the AxioVision Software or by the keyring in Z drive. If a motorised reflector turret is recognised when the microscope is switched, keys are assigned automatically in the following order:Refl.turret to the right (Pos. +), Refl.turret to the left (Pos. -), RL shutter, lamp voltage +, lamp voltage -.Otherwise the default assignment of keys applies:TL shutter, RL shutter, unassigned, lamp voltage +, lamp voltage -.The position indication is shown by the LED Bargraph. As soon as a motorised reflector turret is recognised, the LED Bargraph indicates the reflector turret position, if ”RL on” was set (by pressing button on the Light Control or on the keyring or via Software). If TL is switched on as well (only possible if the toggle switch HAL is on TL), the reflector position will continue to be displayed, overriding the display of the lamp brightness.。
ZEISS Axio Imager 2 开放微观系统，适用于自动材料分析说明书
ZEISS Axio Imager 2Your Open Microscope System for Automated Material AnalysisProduct Information Version 1.2Axio Imager 2 from ZEISS is your system platform tailored to demanding materials analysis tasks, development of new materials as well as quality control.You always profit from crisp images and high optical performance. This applies in particular to sophisticated contrasting techniques, e.g. like the Circular Differential Interference Contrast (C-DIC) and polarization contrast.Use the motorized stand to achieve reproducible illumination settings and, consequently, constant image quality. You always obtain comparable results and high productivity by automating your workflow. Axio Imager 2 offers a high degree of adaptability in line with your future requirements. The stands are open to expand and cover a wide range of applications.Your Open Microscope System for Automated Material Analysis› In Brief › The Advantages › The Applications › The System› Technology and Details ›ServiceAnimation20 µmSimpler. More Intelligent. More Integrated.Profit from an Open Microscope System Whether in research, testing or failure analysis, materials microscopy faces quite various challenges. With Axio Imager 2 from ZEISS you will be able to meet and win these challenges. Attach application-specific components and perform e.g. particle analysis, investigate non-metallic inclusions (NMI), liquid crystals or semiconductor-based MEMs. Axio Imager 2 supports the correlative workflow to electron microscopic investigations, too.Achieve Reliable, Reproducible ResultsStability is essential if you want to obtain good results. You will appreciate the stable imaging conditions of Axio Imager 2, especially when working with high magnifications and performing time dependent studies. Due to the motorization of Axio Imager 2 you will achieve quick and repro-ducible results while you always work under constant conditions. For instance, the motorized apertures and the illumination control, which automatically adjusts the color temperature via filter wheels. Experience Competence in all Contrasting TechniquesChoose from a variety of contrasting techniques to achieve an optimum image quality for your dedicated applications. Examine your samples in reflected light in brightfield, darkfield, Differential Interference Contrast (DIC), Circular Differential Interference Contrast (C-DIC), polarization or fluorescence contrast. For transmitted light you can choose between brightfield, darkfield, Differential Interference Contrast (DIC), polarization or circular polarization. Minimized stray light enables homogenous illumination. You achieve outstanding image contrast, even at high magnifications.Carbon fiber-reinforced polymer (CFRP), Differential Interference Contrast (DIC); Objective: EC Epiplan-NEOFLUAR 50×/0.8Stage insert with correlative sample holder for a big variety of specimen.Appreciate the stable imaging conditions with Axio Imager 2.› In Brief› The Advantages › The Applications › The System› Technology and Details › ServiceBrightfield and Darkfield: Maximum Homogeneity and a Stray Light Free Image Background In brightfield Axio Imager 2 provides homoge-neous illumination and exceptional contrast. By minimizing disturbing stray light and reducing the longitudinal color aberration of the illumination optics, the darkfield illumination contrast is suitable for the most challenging samples and impresses even when faced with finest structures. Switching between the techniques only requires a simple turn. The motorized stands allow you to work particularly quickly and conveniently.C-DIC: Perfect for All StructuresCircular Differential Interference Contrast (C-DIC) is a polarization-optical technique which, in contrast to ordinary Differential Interference Contrast (DIC), uses circularly polarized light. This technique has a number of decisive advantages for the contrasting of differently aligned object structures. The speci-men no longer has to be rotated for best imageExpand Your PossibilitiesCopper casting, brightfield.Objective: EC Epiplan-NEOFLUAR 20×/0.5Copper casting, darkfield.Objective: EC Epiplan-NEOFLUAR 20×/0.5contrast and quality, as it is the case in basic DIC. With C-DIC it is simply enough to adjust the position of the C-DIC prism to achieve best image quality whether it is for contrast and/or resolution independent of sample orientation. And all this is possible using one C-DIC prism for a homoge-neous unsurpassed quality image.Copper casting, C-DIC.Objective: EC Epiplan-NEOFLUAR 20×/0.5Experience Competence in all Contrasting Techniques › In Brief› The Advantages › The Applications › The System› Technology and Details › Service200 µm Experience Competence in All Contrasting TechniquesExpand Your PossibilitiesBrightfieldDarkfieldC-DICSample: pure aluminum; Objective: EC Epiplan-NEOFLUAR 10×/0.25, same position acquired with different contrasting techniquesPolarization Contrast Polarization with Additional Lambda Plate› In Brief› The Advantages › The Applications › The System› Technology and Details › ServiceTailored Precisely to Your Applications› The Advantages› The Applications› The System› Technology and Details› ServiceTailored Precisely to Your Applications› The Advantages› The Applications› The System› Technology and Details› Service20 µm20 µm20 µm20 µm200 µm200 µmZEISS Axio Imager 2 at WorkAviation and Space IndustryCarbon fiber-reinforced polymer (CFRP), brightfield, objective: EC Epiplan-NEOFLUAR 50×/0.8Carbon fiber-reinforced polymer (CFRP), darkfield, objective: EC Epiplan-NEOFLUAR 50×/0.8Carbon fiber-reinforced polymer (CFRP), DIC, objective: EC Epiplan-NEOFLUAR 50×/0.8Raw iron, brightfield,objective: EC Epiplan-NEOFLUAR 50×/0.8Aluminium, polarization,objective: EC Epiplan-NEOFLUAR 10×/0.25Metal Producing and Processing IndustryAluminium, polarization with Lambda plate, objective: EC Epiplan-NEOFLUAR 10×/0.25› In Brief › The Advantages › The Applications › The System› Technology and Details › Service10 µm20 µm50 µmZEISS Axio Imager 2 at WorkOil, Gas and Mining IndustryVitrinite,objective: EC Epiplan-NEOFLUAR 50×/1.0 Oil PolCast iron, brightfield,objective: EC Epiplan-APOCHROMAT 50×/0.95Particle analysis, brightfield,objective: EC Epiplan-NEOFLUAR 20×/0.5Automotive IndustryParticle Analysis› In Brief › The Advantages › The Applications › The System› Technology and Details › ServiceExpand Your PossibilitiesAnalyze Tiny Particles: Accurately and ReproduciblyParticle Analyzer is a milestone for your quality control. With the fully motorized light microscope Axio Imager 2 you measure particles down to 2 µm.Particle Analyzer software supports the standards for cleanliness testing ISO 16232, VDA 19, and oil analysis ISO 4406, ISO 4407, and SAE AS 4059. With the system solutions from ZEISS, you ensure that the required microscope settings are always selected correctly. You receive reliable, reproducible results nearly independent of the user carrying out the analysis. By carrying out correlative particle analyzes, you expand the depth of information contained within your findings to include the results of element and materials characterization.› In Brief › The Advantages › The Applications › The System› Technology and Details › Service100 µmExpand Your PossibilitiesCompletely characterize residual dirt particles with Correlative Automated Particle Analysis from ZEISS. Detect particles with your Axio Imager 2 and relocate preselected particles automatically, using your SEM from ZEISS. Perform an EDX analyisis to reveal information of their elemental composition. Correlative Particle Analyzer automatically documents the results from both, the light microscopic and electron microscopic analysis. You receive a combined, informative report at the touch of a button.As an experienced user, you can inspect the results of the combined light microscopic and electron microscopic analysis on an interactive overview screen. Retrieve particles at the touch of a button, automatically start new EDX analyzes, and auto-matically generate a report. With Correlative Particle Analyzer, your results will be available up to ten times faster than first conducting an analysis with a light microscope and then sub-sequently with an electron microscope. You can systematically focus on potentially process-critical particles.The complementary material characterization from both microscopic worlds gives you added security.Correlative Automated Particle Analysis (CAPA): More Knowledge. Higher Quality.Image of a metallic particle from a light microscopeImage of the same metallic particle from an electron microscopeOverlay of the images from both systems; chemical element composition via EDX analysis; graphical EDX overlay prepared with Bruker Esprit softwareCorrelative sample holder for efficient relocation of particles in your ZEISS scanning electron microscope.› In Brief › The Advantages › The Applications › The System› Technology and Details › Service20 µm 20 µm20 µm20 µmExpand Your PossibilitiesCLEM (Correlative Light and Electron Microscopy) image of a region of interest from an aged Li-ion battery with different contrasts of brightfield (a) and polarized light (b) in LM as well as BSE signal (c) and EDS mapping (d) in SEM.Correlative Microscopy withZEISS Axio Imager 2: Bridging the Micro and Nano WorldAre you looking for a way to combine imaging and analytical methods effectively?Shuttle & Find offers precisely this: An easy-to-use, highly productive workflow from a light to an electron microscope – and vice versa.The workflow between the two systems has never been so easy. The precise recall of regions of interest enhances productivity. Instead of wasting valuable time searching, you now gain new insights into your samples with a few mouse clicks. Regions of interest, marked on one system, you can instantely relocate on the other system. Open up new dimensions of information in numerous material analysis applications. Absolutely reproducible.› In Brief › The Advantages › The Applications › The System› Technology and Details › ServiceExpand Your PossibilitiesExaminations in the fields of research and industrial production (e.g. surface examinations of reflective, low-contrast specimens such as metallographic specimens and polished or textured wafers) require a fast focusing system that ensures high precision levels of max. 0.3 times the objective’s depth of field. This requirement can be easily met by com-bining your Axio Imager 2 with the Auto Focus system to benefit from fast and accurate focusing across a wide capture range of up to 12,000 µm. The Auto Focus system is designed to work with reflected light and transmitted light in brightfield, darkfield, polarized light and DIC.How it WorksThe objective guides the structured light produced by an LED in the Auto Focus system onto the specimen, with the specimen’s surface reflecting it back. During this process, Auto Focus permanently analyses the signal and derives the appropriate control signals for the focus drive, to bring the surface into focus. The Auto Focus sensor detects changes and deviations in the focus position and compensates them automatically. The Auto Focus system comes with three different modes corre-sponding to different specimen characteristics (reflective/partially reflective/diffuse) and with three different precision levels (precision/balance/speed).How the Auto Focus system works: 1) LED 2) Sensor module 3) Sensor 4) Beam splitter 5) O bjective 6) Specimen› In Brief › The Advantages › The Applications › The System› Technology and Details › Service5362141 Microscope• Axio Imager.A2m (encoded)• Axio Imager.D2m (encoded, partly motorizable)• Axio Imager.M2m (motorizable, TL manual)• Axio Imager.Z2m (motorizable, TL motorized)2 Objectives Reflected Light • EC EPIPLAN• EC Epiplan-NEOFLUAR • EC Epiplan-APOCHROMAT Transmitted Light • N-ACHROPLAN • EC Plan-NEOFLUAR • Plan-APOCHROMAT • C-APOCHROMAT • FLUARLong Working Distance • LD EPIPLAN• LD EC Epiplan-NEOFLUAR 3 Illumination Reflected Light • MicroLED • VisLED • Halogen • HBO / HXP Transmitted Light • MicroLED • VisLED • HalogenYour Flexible Choice of Components4 Cameras • Axiocam 105 • Axiocam 305• Axiocam 506• Axiocam 705• Axiocam 7125 Software • ZEN core • ZEN starter6 Accessories • Auto Focus• Linkam heating- and cooling stages • Focus Linear Sensor • Correlative Microscopy› In Brief › The Advantages › The Applications › The System› Technology and Details › ServiceSystem Overview› The Advantages› The Applications› The System› Technology and Details› ServiceSystem Overview› The Advantages› The Applications› The System› Technology and Details› ServiceSystem Overview› The Advantages› The Applications› The System› Technology and Details› ServiceTechnical Specifications› The Advantages› The Applications› The System› Technology and Details› ServiceTechnical Specifications› The Advantages› The Applications› The System› Technology and Details› ServiceTechnical Specifications› The Advantages› The Applications› The System› Technology and Details› ServiceTechnical Specifications› The Advantages› The Applications› The System› Technology and Details› ServiceBecause the ZEISS microscope system is one of your most important tools, we make sure it is always ready to perform. What’s more, we’ll see to it that you are employing all the options that get the best from your microscope. You can choose from a range of service products, each delivered by highly qualified ZEISS specialists who will support you long beyond the purchase of your system. Our aim is to enable you to experience those special moments that inspire your work.Repair. Maintain. Optimize.Attain maximum uptime with your microscope. A ZEISS Protect Service Agreement lets you budget for operating costs, all the while reducing costly downtime and achieving the best results through the improved performance of your system. Choose from service agreements designed to give you a range of options and control levels. We’ll work with you to select the service program that addresses your system needs and usage requirements, in line with your organization’s standard practices.Our service on-demand also brings you distinct advantages. ZEISS service staff will analyze issues at hand and resolve them – whether using remote maintenance software or working on site. Enhance Your Microscope System.Your ZEISS microscope system is designed for a variety of updates: open interfaces allow you to maintain a high technological level at all times. As a result you’ll work more efficiently now, while extending the productive lifetime of your microscope as new update possibilities come on stream.Profit from the optimized performance of your microscope system with services from ZEISS – now and for years to come.Count on Service in the True Sense of the Word>> /microservice› In Brief › The Advantages › The Applications › The System› Technology and Details › ServiceCarl Zeiss Microscopy GmbH 07745 Jena, Germany******************** /axioimager-mat Notfortherapeuticuse,treatmentormedicaldiagnosticevidence.Notallproductsareavailableineverycountry.ContactyourlocalZEISSrepresentativeformoreinformation.EN_42_11_31|CZ11-219|Design,scopeofdelivery,andtechnicalprogresssubjecttochangewithoutnotice.|©CarlZeissMicroscopyGmbH。
ZEISS Axio Imager 2研究显微镜说明书
Pilidium larvae of the Nemertean ribbon worm,Cerebratulus lacteus. Courtesy of the Marine BiologyLaboratory & Development journalEsophagus (guinea pig)ZEISS Axio Imager 2Your Upright Research Microscope for Advanced ImagingAxio Imager 2 combines everything you’veever asked for in your research environment:brilliant optics, bright fl uorescence and vari-ous methods of transmitted light contrast.The contrast manager and light manager en-sure defi ned conditions and reproducible re-sults at all times. With your Axio Imager.Z2you can use ACR to automatically detect andconfi gure objectives and fi lter sets.Choose your system out of six different standversions. Whether you are simply observingand recording, or performing highly compleximaging experiments, it’s easy to adapt thesystem components to the application athand. The research microscope Axio Imager2 resolves fi nest details of your samples -from tissue sections in pathology to brainspecimens in neuroscience to multi-colorFISH samples.Highlights• Encoding: read-out magnifi cation,illumination and contrast settings, andautomatic transfer to ZEN Imagingsoftware• Motorization for reproducible compo-nent settings and automated processes• Excellent optics and homogeneousillumination in both transmitted lightand fl uorescence applications• Research microscope with high perfor-mance focus for maximum precisionand 24/7 operation• Smart control concept for ergonomicworking conditions and multi-usermanagement for up to ten differentusers or working scenarios.• Extend your Axio Imager 2 with high endfl uorescence imaging systems, such asApotome.2, LSM 800 and LSM 880Ease of UseUse your motorized Axio Imager 2 withequipment tailored precisely to yourneeds - and upgrade your system easilyat any time.The smart automated contrast settingslet you work quickly and effi ciently. Themotorized aperture and luminous fi elddiaphragm and light intensity areautomatically adjusted. Achieve repro-ducible results and control all componentsvia optional TFT monitor.***************/axioimagerN o t a l l p r o d u c t s a r e a v a i l a b l e i n e v e r y c o u n t r y . U s e o f p r o d u c t s f o r m e d i c a l d i a g n o s t i c , t h e r a p e u t i c o r t r e a t m e n t p u r p o s e s m a y b e l i m i t e d b y l o c a l r e g u l a t i o n s . C o n t a c t y o u r l o c a l Z E I S S r e p r e s e n t a t i v e f o r m o r e i n f o r m a t i o n .E N _41_012_113 | C Z 08-2015 | D e s i g n , s c o p e o f d e l i v e r y a n d t e c h n i c a l p r o g r e s s s u b j e c t t o c h a n g e w i t h o u t n o t i c e . | © C a r l Z e i s s M i c r o s c o p y G m bHPerformance:The motorized reflector turret accommo-dates either six or ten Push & Click filtermodules. Auto-configure all motorized components with Smart Setup of the ZEN imaging software.Acquire fluorescence images with an excellent signal-to-noise ratio. The fluorescence beam path and high efficiency fluorescence filter sets of this research microscope deliver exposure times that are up to 50 percent shorter.Stand Versions:• Axio Imager.A2 LED • Axio Imager.A2• Axio Imager.D2• Axio Imager.M2p • Axio Imager.M2• Axio Imager.Z2 Suitable Applications:• Cell biology • Neuroscience • Molecular genetics • PathologyNote: This product is primarily for research use. Only Axio Imager.M2p is for use in diagnostic procedures or patient management.ZEISS Axio Imager 2Your Upright Research Microscope for Advanced Imaging。
最新AxiIMAGER说明(中英对照)汇总
A x i I M A G E R说明(中英对照)蔡司图像分析系统Carl Zeiss Imaging Analysis Systems北京普瑞赛司仪器有限公司BEIJING PRECISE INSTRUMENT CO., LTDAxio ImagerA1M技术说明Axio ImagerA1M Technical Specifications用户名称：Username:日期：2009-4-30Date: 2009-4-30尊敬的杜工您好！首先感谢贵方的询问及询价！我方根据贵方对材料检验研究的最新要求，推荐ZEISS顶级研究级倒置万能材料显微镜Axio ImagerA1MDear Manager Du,Hello!First of all, thank you for your Inquiries!According to your refreshed requirements on Materials analysis, we recommend Axio Imager A1M to you.品牌介绍：世界顶级品牌，可见光及电子光学领导企业-----蔡司是一家致力於应用研究，对於光学、玻璃技术、精密技术以及电子等高品质的产品开发、制造、销售有着突出贡献的德国军工企业。
自1846 年开始，carl zeiss已有160多年的传统与创新。
百年历史缔造了蔡司在光学领域不可撼动的领导地位，至今显微镜的生产标准中的83%是以蔡司厂标为基准。
国际物镜的检测标准是以蔡司物镜为基准。
蔡司显微镜以其不断领先的技术和可靠的质量推动了世界材料科学的发展同时也受到知名科学家和诺贝尔奖得主的青睐！作为显微镜的鼻祖国际标准的缔造者，蔡司公司将以更新的技术延续carl zeiss成功的传奇故事！Brand Introduction:The world's top brand, the leading enterprises of visible light and electron optical——Carl Zeiss is a German military enterprises with outstanding contributions to the development, manufacturing and sales of optics, glass technology, microtechnic and electronic products. Since 1846, Carl Zeiss has a history of tradition and innovation for 160 years. The nearly I00 years history has established the leadership of Carl Zeiss. Up to now, 83% of the microscope production use Zeiss criterion and the international objective testing standard are based on Carl Zeiss. Zeiss microscopes have also win favor of famous scientists and Nobel Prize winner by its technology and quality. As the originator of the microscope international standards, Carl Zeiss will update its technology constantly and carry on the legend!设备名称：德国蔡司金相显微镜Device Name: ZEISS Metallographic Microscopy (Germany)规格型号: Axio ImagerA1MModel: Axio ImagerA1M用途:对钢铁有色金属等材料的显微组织观察和分析。
激光共聚焦系统使用说明 Imager Vario LSM
共聚焦显微镜操作入门指南(仅供内部使用)文档作者：李宇(liyu@) 日期：2012-12-04文档校对：李宇日期：2012-12-04卡尔蔡司中国版权所有不得复制目录1开机 (1)1 .1接通总电源 (1)1 .2打开激光器 (1)1 .3打开控制器、主控电脑 (1)2使用激光共聚焦扫描软件Zen 2010 (2)2 .1打开软件 (2)2 .2切换到明场观察模式（目镜筒） (2)2 .3放入样品并在明场模式下找到焦平面 (3)2 .4切换到共聚焦扫描模式 (6)2 .5设置激光扫描参数，找到样品最亮的焦平面位置 (6)2 .6设置Z-stack扫描上下限 (8)2 .7开始扫描 (10)2 .8分析扫描结果，进行三维观测 (11)3关机 (15)4附：目镜中，使用明场、暗场和偏光模式观察样品 (16)4 .1明场模式 (16)4 .2暗场模式 (17)4 .3偏光模式 (17)5附：使用相机（CCD）拍摄明场、暗场和偏光图 (18)5 .1拍摄明场图 (18)5 .2拍摄暗场图 (19)5 .3拍摄偏光图 (19)1 开机1 .1接通总电源图 1 从左至右依次为：墙体总电源、稳压器电源、激光器和电脑电源1 .2打开激光器图 2 转动激光器钥匙，打开激光器，LED指示灯亮1 .3打开控制器、主控电脑图 3 依次打开左图显微镜主机控制器电源、右图电脑主机电源提示：当仅使用CCD拍图，或者长时间不用机器时，建议关闭激光器，以延长其寿命。
2 使用激光共聚焦扫描软件Zen 20102 .1打开软件双击图标，然后点击“Start System ”进入软件。
2 .2切换到明场观察模式（目镜筒）2 .2.1 在共聚焦软件中切换为明场观察模式：点击“Locate ”标签，选择“Online ”，点击“BF ”（Bright Field 的缩写）。
此时系统打开卤素灯，并将明场光学模块转入光路。
图 4 切换为明场观察模式提示：如果出现硬件通讯问题，软件左下角会弹出信息对话框，此时一般的解决方法是：1）重启Zen 软件；2）如果仍无效，关闭整个系统，过5分钟后再重启系统。
Axio Imager M2显微镜使用手册
8、调节荧光光强
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9、调节焦距
显微镜主机的左右两侧都有调焦旋钮
左侧调焦旋钮 外圈为粗调，里圈为微调
微调
粗调
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右侧调焦旋钮 外圈为粗调，里圈为微调
粗调
微调
ZeiSsseitWe angying
10、移动载物台
通过此手柄移动电动载物台
荧光观察
ZeiSsseitWe angying
1、打开电源
按下开关，打开显微镜总电源
ZeiSsseitWe angying
2、打开显微镜开关
按下显微镜主机体左下方开关，打开显微镜
ZeiSsseitWe angying
1、打开荧光灯源
ZeiSsseitWe angying
1、打开显微镜开关后，显微镜右侧电子 显示屏会启动,
2、直到出现此界面，显微镜启动完成 3、白色为激活状态，如Home等
注意：准备好样本和选择好滤 光块前，此状态一定为off
ZeiSsseitWe angying
3、降低载物台
点击触摸屏上“Load position”，载 物台下降
ZeiSsseitWe angying
4、放入样本
1，将样品放入样本夹并夹紧
ZeiSsseitWe angying
明场观察
ZeiSsseitWe angying
1、打开电源
按下开关，打开显微镜总电源
ZeiSsseitWe angying
2、打开显微镜开关
按下显微镜主机体左下方开关，打开显微镜
ZeiSsseitWe angying
1、打开显微镜开关后，显微镜右侧电子 显示屏会启动,
“一枝独秀”之馆藏曲柄铜盉的科学分析与考古初探
“一枝独秀”之馆藏曲柄铜盉的科学分析与考古初探作者：穆浴阳李文欢来源：《文物鉴定与鉴赏》2024年第03期摘要：利用掃描电镜-能谱仪（SEM-EDS）及金（矿）相显微镜等研究手段，对江西九江共青城市博物馆馆藏唯一一件曲柄铜盉进行金相组织观察和成分检测分析，并初步进行考古研究。
结果表明：此曲柄铜盉的材质为铜-锡-铅（Cu-Sn-Pb）三元合金，制作工艺为铸造而成，其成熟的铜锡铅配比和加工方法佐证了商周青铜器的铸造技术。
虽然曲柄铜盉的出土地为江西地区，但是通过形制初步判断其为群舒文化典型青铜盉，在一定程度上说明了吴、楚、越文化和群舒文化在先秦时期的融合情况。
截至目前，此曲柄铜盉在江西地区尚属首次和唯一出现，填补了江西此类型青铜器研究的空白，对于揭示江西先秦青铜器冶金技术内涵及探讨江西与其周边地区的交流传播具有重要的价值意义。
关键词：江西先秦古史；西周春秋时期；吴楚越文化；群舒文化DOI：10.20005/ki.issn.1674-8697.2024.03.0270 引言在人类技术发展过程中，自使用青铜器伊始，称为青铜时代。
中国的青铜时代始于公元前2000年左右，历经夏商周时期。
青铜器的使用随着商周时期青铜冶铸业的发展达到鼎盛，其产生的青铜艺术亦是亚洲大陆上一颗璀璨的明珠①。
江西的考古自中华人民共和国成立后逐步发展，商周考古取得了较大的成绩，这些大量古文化遗存和丰富的文化遗物作为“无字地书”对于探寻江西先秦古史的意义重大②。
随着江西考古的发展，出土青铜器等文物逐步问世，其种类繁多、遍布全境，年代上溯商周下至汉代。
在江西省九江市共青城市博物馆中，馆藏的唯一一件青铜器—曲柄铜盉较为特殊，其为截至目前该地区出土青铜器中首次且唯一出现的青铜盉类型，不仅填补了江西地区此类型青铜器的空白，更是对于研究江西先秦古史具有重要的价值。
本文拟通过对江西省九江市共青城市博物馆馆藏曲柄铜盉进行科学分析，初步揭示其制作工艺及冶金技术内涵，同时为先秦时期江西与其周边地区交流和发展的研究提供实物证据。
Axio Imager M2显微镜使用手册
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Zeiss Wangying
明场观察
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Zeiss Wangying
1、打开电 源
按下开关，打开显微镜总电源
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Zeiss Wangying
2、打开显微镜开 关
按下显微镜主机体左下方开关，打开显微镜
Seite
Zeiss Wangying
1、打开显微镜开关后，显微镜右侧电子 显示屏会启动,
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Zeiss Wangying
2、打开显微镜开 关
按下显微镜主机体左下方开关，打开显微镜
Seite
Zeiss Wangying
1、打开显微镜开关后，显微镜右侧电子 显示屏会启动,
2、直到出现此界面，显微镜启动完成
3、白色为激活状态，如Home等
Seite
Zeiss Wangying
3、降低载物 台
点击触摸屏上“Load position”， 载 物台下降
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Zeiss Wangying
4、放入样 本
1，将样品放入样本夹并夹紧
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Zeiss Wangying
5、升高载物 台
点击触摸屏上红色箭头所示 按钮，载物台会自动上升到 原来位置
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Zeiss Wangying
6、选择物 镜
左侧调焦旋钮 外圈为粗调，里圈为微调
微调
粗调
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Zeiss Wangying
右侧调焦旋钮 外圈为粗调，里圈为微调
粗调
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微调
Zeiss Wangying
10、移动载物 台
通过此手柄移动电动载物台
Zeiss Wangying
Zeiss axio image z1
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另外一种用于Axio Imager.Z1上的Z轴马达，其步距为10 nm，重复性为 ± 10 nm，同时速度加快了3倍。闭合环路系统 —— 高精度调焦如果您对调焦 精确程度有特别高的要求，装配有线性调焦传感器的 Axio Imager.Z1 可以在 Z轴方向上达到 ± 1nm 的精确度。一方面，传感器可探测显微镜载物台发生 的与实验无关的移动并做出相应的调整，另一方面，该系统通过 Z 轴方向上 相同的步距确保了极高的精确性以及三维序列的重复性，可提供给您最大程 度上的控制的可靠性。 "Imaging Cell" —— 无震动观测 Axio Imager的核心 组成部分，如物镜转换器、调焦系统以及载物台，已经作为一个整体——“稳 定核心”——与显微镜主机架分离。该系统的设计使其不受震动的影响，并 且很长时间内不受外界温度变化的影响。因此，它为成像，特别是长时间时 间序列图像的采集过程创造了一个很好的条件。在工作中得到完全的放松 卡 尔·蔡司为 Axio Imager 设计了一个超前的工作理念并且最大程度的简化了许 多功能的操作过程。我们的目标是将您从长时间、繁重的工作压力中解放出 来，从而让您完全把精力投入实验之中。所有的这一切皆得益于可以直观操 作的技术，并不取决于电动或者手动。触控屏 —— 关键信息一览无遗 化繁 为简，电动 Axio Imager 首次将所有功能的操控集成在TFT液晶显示屏上： 您能十分便捷的通过触击显示屏上的按钮来操控所有的电动化
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色温 • 发光二极管照明已成为替代传统卤素灯照明的最佳方案。它具有诸多关键性的优点：恒定的， 不受亮度影响的色温，低热辐射及高耐久性。发光二极管照明系统包括一个滤片座，以满足您对于 不同色温的个别需求。它可被直接固定在聚光镜下方，形成固定式科勒照明，简化了各种反差技术 下的调节过程。发光二极管照明也可以安装在传统卤素灯的位置以提供完全的科勒照明。 • 完美的 微分干涉 —— 更均匀的照明 • Axio Imager 可以在从5倍到100倍的不同视野范围内保持均匀的干涉 反差。这样，特别对于数码成像，您无需再进行费时的阴影校正处理。另一个关键优点是，您可以 为 Plan-Apochromat 63x/1.4 和 100x/1.4 物镜选择两种不同的DIC插片：HR可提供最高分辨率， HC 则带来最佳反差效果——可以更加适宜的迎合您的应用。转换更快的荧光滤色镜转盘 —— 6 孔 或10孔速度是荧光技术中的一个关键问题。卡尔·蔡司针对此问题，量身定做了一系列组件：高速 的，电动的，可装载6个即插即用荧光滤色镜模块的转盘，用于快速的多通道荧光成像，节省时间， 避免不必要的荧光漂白。如果您想同时使用多于6种的染料，如M-FISH应用，可快速转换的10孔电 动荧光滤色镜转盘将能够帮助您取得最佳的效果。即使是极微弱的荧光，您也可以采集到完美的、 无漂移的信号。电动光阑 —— 可靠的重复性在反射和透射光路中，电动的智能化孔径光阑及视场 光阑都可自动调节反差及照明效果。比如，根据所用物镜的光圈调节可在任何时间进行记录或者调 用。这意味着轻触一键即可完成观察条件的恢复。高效滤色镜组——无比的亮度高光效（HE）荧 光滤色镜是首次随 Axio Imager 推出，它显著地提高了荧光信噪比。由于更高的激发光、发射光透 过率以及更精确的波长选择边界，荧光信号的分离效果更好，效率更高。这缩短了多达50%的曝光 时间，从而使您的样品得到保护。自动定心汞灯 —— 可重复的荧光专为用户着想，方便实用：可 自动定心的汞灯。在每次更换灯泡或者启动后，汞灯会自动定心。这确保了稳定的、优化的设置及 视野范围内的均匀照明，也保证了在整个汞灯使用寿命内的可重复性。AxioVision —— 数码智能 AxioVision 高性能软件是
蔡司 Axioscope 显微镜产品资料说明书
产品资料版本1.0蔡司Axioscope材料实验室用于日常工作和研究的显微镜Axioscope 正置式光学显微镜专为材料实验室最常见的光学成像要求而设计。
具备带编码和自动化功能，特别适合于对数据质量和可重复性要求较高的检测工作。
但 Axioscope 的功能并不只有这些。
它还善于进行材料科学研究中的高级光学显微分析。
Axioscope 可以对晶粒尺寸、物相含量以及膜层厚度进行测量，还可对石墨颗粒进行评级，为科研与工业中的金相学和材料科学提供了一套完整的解决方案。
具有多种成熟的观察模式可以分析您的样品。
先进的照明管理可以确保您的样品始终处于优化的照明状态。
Axioscope 功能多样，处理日常工作得心应手，是实验室检测设备的理想之选。
全力服务于研究和日常检测› 概述› 优势› 应用› 系统› 技术与详细介绍› 服务更简单、更智能、更集成经济实惠，性能卓越材料实验室的工作特点在于结合了常规的日常任务和具有挑战性的高级分析任务。
当需要高性能成像和更丰富的观察方式时，适合于常规应用的显微镜会迅速达到性能极限，但另一方面，昂贵的研究级显微镜所提供的丰富功能有时候也经常会被束之高阁。
Axioscope 具有出色的用途多样性和先进的自动化功能，是要求苛刻的日常工作的理想选择。
它的价格极具吸引力，并提供通常只有更先进的研究级光学显微镜才配有的强大功能。
数字集成选择蔡司的理由之一便是其全方位的集成平台，可以连接所有蔡司显微镜的数据。
将 Axioscope 与蔡司 Axiocam 系列相机和蔡司 ZEN 2 core 成像软件相结合，Axioscope 如今能够成为一套功能强大的数字记录系统。
从设备控制到图像拍摄、从分析记录到归档您宝贵的分析数据，Axioscope 提供完全数字化的工作流程。
此外，Axioscope 还可以通过 Shuttle & Find 集成到关联工作流程中，提供与电镜以及其他显微成像设备关联分析同一样品的可能。
ZEISS Axio Observer 逆向显微镜系统说明书
ZEISS Axio ObserverYour Inverted Microscope System for MetallographyProduct InformationVersion 1.050 µmSpherulitic graphite in nodular cast iron seen in C-DIC contrast.Your Inverted Microscope System for MetallographyFast, flexible, economic: Take advantage of Axio Observer’s inverted construction to investigate a large number of samples in no time at all – or to explore heavy ones, just as efficiently. There’s no need to refocus, even when changing magnifi-cation or switching samples. Axio Observer combines the proven quality of ZEISS optics with automated components to give you reliable, reproducible results. Using dedicated software modules you can analyze, for example, non-metallic inclusions, grain sizes and phases – it’s fully automatic. Axio Observer is your open imaging platform: invest in only the features you need today.As requirements change, a simple upgrade keeps your system ready for all materials applications.› In Brief › The Advantages › The Applications › The System› Technology and Details ›ServiceAnimationSimpler. More intelligent. More integrated.Save Time in Metallographic Investigations As an inverted microscope platform, Axio Observer makes work so much more enjoyable. Whether investigating a large number or even heavy samples, you’ll save time in both sample preparation and investigation. Meanwhile, its inverted design facilitates parallel alignment to the objective lens. Observe more samples in less time: simply put your specimen on the stage, focus once and keep the focus for all further magnifications and samples.Upgrade Your SystemKeep an eye on your budget as well as your samples.With Axio Observer, you invest only in the featuresyou need now. You can always upgrade your system,simply and economically, any time you need to.Choose between encoded or motorized compo-nents and a range of accessories – you can dependon having any relevant contrasting techniques yourapplication requires.Count on Reliable Results and Brilliant ImagesYou will appreciate the stable imaging conditionsof Axio Observer, especially when working withhigh magnifications. Homogeneous illuminationacross the entire field of view produces brilliantimages. And you will get reliable, reproducibleresults every time, thanks to the proven opticalquality of ZEISS combined with automated com-ponents. Profit from short time-to-image for yourmetallographic structure analysis with dedicatedsoftware modules, e.g. NMI, Grains, Multiphase.› In Brief› The Advantages› The Applications› The System› Technology and Details › ServiceExpand Your PossibilitiesChoose Between Three Different Stands• Control all motorized components of yourAxio Observer 7 materials with its touchscreendisplay. Automatic Component Recognition(ACR) means it will always recognize the settingsfor objectives and filtersets you have chosen.• Axio Observer 5 materials – nearly all compo-nents can be read out or even motorized• Axio Observer 3 materials with an encodednosepiece, light manager, CAN and USB inter-face that enables a read-out of the magnificationGet Crisp Images with Polarization ContrastInvestigate your samples with polarization contrastusing fixed analyzers, a measuring analyzer rotatingthrough 360° and a rotating analyzer with rotatingfull-wave plate.Now, you can also use a rotatable polarizer tochange the direction of incidence of the polarizedlight. This also makes bireflection and pleochroismvisible on anisotropic samples. In addition, someore phases display anisotropy in the polarized re-flected light, whereby a color change is generateddepending on the placement of the polarizer a fewdegrees +/- from the marked position.Take Advantage of a Variety of Stage InsertsSelect from a variety of stage inserts to tailor thesystem to your needs. The high-grade spring steelwill not yield under loads, even when examiningmany samples. Thus you can be sure that the opticalreference plane is maintained. Stage inserts comewith different inside apertures to match standardspecimen diameters, plus a 10 mm aperture forvery small specimens.› In Brief› The Advantages› The Applications› The System› Technology and Details› ServiceTailored Precisely to Your Applications› The Advantages› The Applications› The System› Technology and Details› Service50 µm50 µm50 µm50 µmBrightfieldDarkfieldPolarization ContrastPolarization with Additional Lambda PlateZEISS Axio Observer at WorkSpherulitic graphite in nodular grey cast iron, spheruliths with ferrite envelope and perlitic ground mass, same position acquired in reflected light with different contrasting techniques, objective: EC Epiplan-NEOFLUAR 50×/0.80 HD DIC› In Brief › The Advantages › The Applications › The System› Technology and Details › Service50 µm50 µm50 µm50 µm50 µmCast aluminum-silicon, reflected light, brightfield, objective: EC Epiplan-NEOFLUAR 20×/0.50 HD DICZEISS Axio Observer at WorkCast aluminum-silicon, reflected light, darkfield, objective: EC Epiplan-NEOFLUAR 20×/0.50 HD DICNiccolite, reflected light, polarization contrast with lambda plate, objective: EC Epiplan-NEOFLUAR 20×/0.50 HD DICZinc, reflected light, polarization contrast with lambda plate, objective: EC Epiplan-NEOFLUAR 20×/0.50 HD DICNiccolite, reflected light, polarization contrast with slightly twisted polarizers, objective: EC Epiplan-NEOFLUAR 20×/0.50 HD DIC› In Brief › The Advantages › The Applications › The System› Technology and Details › Service500 µm500 µm500 µm500 µmBarker-etched aluminum, reflected light, polarization contrast, objective: EC Epiplan-NEOFLUAR 5×/0.13 HD DICZEISS Axio Observer at WorkBarker-etched aluminum, reflected light, polarization contrast with lambda plate, objective: EC Epiplan-NEOFLUAR 5×/0.13 HD DICBarker-etched aluminum, reflected light, circular polarization contrast, objective: EC Epiplan-NEOFLUAR 5×/0.13 HD DICBarker-etched aluminum, reflected light, differential interference contrast with circular polarized light (C-DIC), objective: EC Epiplan-NEOFLUAR 5×/0.13 HD DIC› In Brief › The Advantages › The Applications › The System› Technology and Details › Service1234561 Microscope• Axio Observer 3 materials (encoded)• Axio Observer 5 materials (encoded, partly motorized)• Axio Observer 7 materials (motorized)2 Objectives • EC Epiplan• EC Epiplan-NEOFLUAR • EC Epiplan-APOCHROMATYour Flexible Choice of Components3 Illumination Reflected light:• microLED • HAL 100• HBOTransmitted light:• HAL 100• microLED4 Cameras • Axiocam HRc • Axiocam MRc 5• Axiocam MRc • Axiocam 506 color • Axiocam 503 color • Axiocam ICc 5• Axiocam ICc 1• Axiocam 105 color5 Software • AxioVision • AxioVision LE • ZEN 2 core • ZEN 2 starter6 Accessories• Correlative Microscopy • Fixed, measuring,rotating analyzer and polarizers • Gliding stage, scanning stages› In Brief › The Advantages › The Applications › The System› Technology and Details › ServiceSystem Overview› The Advantages› The Applications› The System› Technology and Details› ServiceSystem Overview› The Advantages› The Applications› The System› Technology and Details› ServiceTechnical Specifications› The Advantages› The Applications› The System› Technology and Details› ServiceTechnical Specifications› The Advantages› The Applications› The System› Technology and Details› ServiceTechnical Specifications› The Advantages› The Applications› The System› Technology and Details› ServiceTechnical Specifications› The Advantages› The Applications› The System› Technology and Details› ServiceTechnical Specifications› The Advantages› The Applications› The System› Technology and Details› ServiceTechnical Specifications› The Advantages› The Applications› The System› Technology and Details› ServiceTechnical Specifications› The Advantages› The Applications› The System› Technology and Details› ServiceBecause the ZEISS microscope system is one of your most important tools, we make sure it is always ready to perform. What’s more, we’ll see to it that you are employing all the options that get the best from your microscope. You can choose from a range of service products, each delivered by highly qualified ZEISS specialists who will support you long beyond the purchase of your system. Our aim is to enable you to experience those special moments that inspire your work.Repair. Maintain. Optimize.Attain maximum uptime with your microscope. A ZEISS Protect Service Agreement lets you budget for operating costs, all the while reducing costly downtime and achieving the best results through the improved performance of your system. Choose from service agreements designed to give you a range of options and control levels. We’ll work with you to select the service program that addresses your system needs and usage requirements, in line with your organization’s standard practices.Our service on-demand also brings you distinct advantages. ZEISS service staff will analyze issues at hand and resolve them – whether using remote maintenance software or working on site. Enhance Your Microscope System.Your ZEISS microscope system is designed for a variety of updates: open interfaces allow you to maintain a high technological level at all times. As a result you’ll work more efficiently now, while extending the productive lifetime of your microscope as new update possibilities come on stream.Profit from the optimized performance of your microscope system with services from ZEISS – now and for years to come.Count on Service in the True Sense of the Word› In Brief › The Advantages › The Applications › The System› Technology and Details › ServiceE N _42_011_176 | C Z 10-2015 | D e s i g n , s c o p e o f d e l i v e r y , a n d t e c h n i c a l p r o g r e s s s u b j e c t t o c h a n g e w i t h o u t n o t i c e . | © C a r l Z e i s s M i c r o s c o p y G m b HCarl Zeiss Microscopy GmbH 07745 Jena, Germany ********************。
金相显微镜Axio Imager M2m
研究级智能全自动万能金相显微镜Axio Imager M2mZEISS一百多年的骄人历史从发明世界上首台显微镜开始。
一个世纪后的今天，ZEISS仍致力于为用户研发最具创造力的显微镜系列产品。
通过我们不断改进的显微技术，我们正在为全世界的用户开拓一条探索微观世界的道路。
今天的显微镜与以往相比，它们的成像质量更好、效率更高、机械性能更加稳定，并且更加环保。
总体描述：金相学主要指借助光学（金相）显微镜和体视显微镜等对材料显微组织、低倍组织和断口组织等进行分析研究和表征的材料学科分支，既包含材料显微组织的成像及其定性、定量表征，亦包含必要的样品制备、准备和取样方法。
其主要反映和表征构成材料的相和组织组成物、晶粒（亦包括可能存在的亚晶）、非金属夹杂物乃至某些晶体缺陷（例如位错）的数量、形貌、大小、分布、取向、空间排布状态等。
金相学的兴起给金属材料研究带来了历史性的变革，而蔡司长久以来一直致力于金相显微镜的研发与应用，并将金相学的科研水平推向一个又一个高点。
2010年蔡司最新推出的金相显微镜Axio Imager M2m再次为金相学的长足发展了提供最佳检测工具。
旷世经典、延续传奇！蔡司研究级智能全自动正立万能材料显微镜Axio Imager M2m的诞生源于蔡司高端智能显微镜Axio Imager M1m升级产品，在研究级智能全自动万能材料显微镜Axio Imager M1m卓越的产品性能基础上，对光路设计尤其是照明系统进行了全新的升级，将光学系统的优化发挥到了极致，展现给您无微不至的细节和最锐利的显微图像。
Axio Imager M2m可通过AxioVision软件、TFT液晶显示屏、远程、手动等方式进行显微镜的所有操作控制，操作菜单和所采集的图像及显微镜功能控制同界面，是至今为止智能化程度最高的研究级显微镜。
Axio Imager M2m的诞生给智能化显微镜提出了全新的标准，将蔡司的显微技术又一次推向了巅峰。
ZEISS ZEN 软件用户指南说明书
Guided Acquisition: Automate your microscopy -Detect rare events with easeZEN Guided Acquisitionfor Life Sciences Applications/ZEN1. Overview ScanThe purpose of the overview scan is to quickly tile-scan large areas using low magnification objectives and fast imaging settings (e.g. single DAPI channel using a camera with short exposure time and 2x2binning). The image quality of this overview scan must be just goodenough, for the following image analysis step to reliably detect the objects of interest. Imaging parameters for theoverview scan can be adjusted and saved into one“Experiment” setting. The focusing strategy can be specified as part of the “Experiment” setting complemented by additional Guided Acquisition options.Both the hardware focusing deviceDefinite Focus 2 and Software Autofocus can combined for highest flexibility. An optional image processing step allows to perform, e.g. Airyscan processing or shading correction, of the overview scan prior to Object Detection if necessary.2. Object DetectionFor the detection of objects of interest in the overview scan, Guided Acquisition uses the powerful and flexible ZEN Image Analysis module. Objects are isolated by image segmentation, using algorithms based on global thresholding, local variance, or Machine Learning (requires additionally the ZEN Intellesis module).ZEN Module Guided AcquisitionAutomate your microscopy, detect rare events with easeRare event detection in high demandIn life science research it is often necessary to selectively examine specific objects from a large population, e.g. to identify and selectively image a few dividing cells in a petri dish, to trace one specific neuron in a sectioned brain slice, or to acquire a 3-dimensional volume of cultured organoids with a certain size and shape. Such experiments are usually time consuming and prone to bias depending on the individual operator, especially if the events happen rarely. The ZEN Module Guided Acquisition has been designed to simplify thisprocess by combining microscopy automation with image analysis. It can be used with multiple ZEISS imaging platforms such as Axio Observer 7 with scanning stage, Celldiscoverer 7, or LSM 980 with Airyscan 2.Guided Acquisition Workflow1. Scan a large area with lowmagnification and fast imaging modality 2. Perform a pre-defined image analysis to detect objects of interest3. Acquire detailed images for every detected object using specified settings Once the Guided Acquisitionworkflow is optimized for a given sample, all settings can be saved and reused for another similar sample with one simple click.Additional filtering refines the list ofdetected objects based on their intensity,size or shape. Image analysis can be performed on both multi-channelfluorescent images and RGB color images,with various bit-depths. For downstream Detailed Acquisition, the location (X/Y scanning stage coordinates) and size (X/Y bounding box) of the detected objects is automatically recorded.3. Detailed AcquisitionThe third step consists of a different set of "Experiment" settings, typically with high magnification, high resolution, and multiple dimensions, which is performed for each detected object. If the size of a detected object is larger than a single field of view, a tile scan will beautomatically configured, based on its bounding box size. All objects that were previously detected by the image analysis step will be acquired sequentially based on their stage coordinates. For each object, a different focus offset can be defined to accommodate samples with differing depths.At the end of the workflow, all images (overview scan and detailed acquisitions)and settings (experiment, processing and analysis settings, and tables of detected objects) will be stored in one folder foreasy access.Low magnification Large areaHigh throughputImage Analysis High specificity High efficiencyHigh resolution Multi-dimension Full flexibility2Guided Acquisition in ActionMitotic Cell Detection from Petri Dish In this example, porcine kidney cells (LLC-PK1) were cultured in a 35 mm glass bottom petri dish. The nuclei were labeled with Histone 2B mCherry, and microtubles with tubulin mEmerald. The goal was to detect the mitotic cells in the population. The experiment was performed using ZEISS Celldiscoverer 7. The overview scan was acquired with a Plan-Apochromat 5x/0.35objective, 1x magnification changer,and the Axiocam 506 mono; the detailed acquisition was performed with a Plan-Apochromat 50x/1.2water immersion objective, 0.5x magnification changer, and Airyscan MPLX HS mode. Image Analysis was performed on the nuclear channel,where mean intensity and area were used to detect the mitotic cells.Sample obtained from ZEISS Oberkochen demo labLabeled neuron detection from mouse brain sectionsIn this example, 15 sectioned mouse brains were prepared on a standard microscope glass slide. The nuclei were labeled with DAPI, and the cells-of-interest are cortical interneurons which expressmembrane Tdtomato by low titre retroviral infection. The experiment was conducted using ZEISSCelldiscoverer 7. The overview scan was acquired with a Plan-Apochromat5x/0.35 objective, 0.5x magnification changer, and the Axiocam 506mono; the detailed acquisition was performed with a Plan-Apochromat 20x/0.95 objective, 0.5xmagnification, Airyscan MPLX HS mode, and Z-stacks (figure shows maximum intensity projection of the detected neuron). Image Analysis was performed on the neuronal channel,where mean and range of intensity were used for detection.Sample courtesy of Dr. L. Lim, Katholieke Universiteit Leuven/VIB Center for Brain & Disease Research,BelgiumDrosophila embryo detection with lateral oriented gut structure from a prepared slideIn this example, a group of fixed drosophila embryos were prepared on a standard microscope glass slide.Longitudinal visceral muscles (one type of gut muscles) were labeled with Alexa 488, and Cut (one type of homeodomain transcription factor)with Cy3. The experiment wasperformed using ZEISS Celldiscoverer 7. The overview scan was acquired with a Plan-Apochromat 5x/0.35objective, 0.5x magnificationchanger, and the Axiocam 506 mono;the detailed acquisition wasperformed with a Plan-Apochromat 20x/0.95 objective, 0.5xmagnification changer, Airyscan MPLX HS mode, and Z-stacks (figure shows maximum intensity projection of the detected embryo). Image Analysis was performed on the gut structure, where green positiveembryos were detected first by mean intensity, then filtered by geometric features to identify those with preferred lateral orientation.Sample courtesy of Dr. G. Wolfstetter, University of Gothenburg, Germany3Guided Acquisition is available for multiple platformsDefinite Focus 2 is recommended for Axio Observer 7*Front page image shows Guided Acquisition for detection of cell-cell interaction between mammalian U2OS cells expressing late endosome (Rab5-mEmerald) or actin (lifeAct-tdTomato). Sample from ZEISS Oberkochen demo lab4Hardware Requirements:Axio Observer Z1/7Axio Imager M1/M2/Z1/Z2Axio Examiner Axioscope 7Axio Zoom.V16Celldiscoverer 7 (with LSM 900)LSM 800 (with Airyscan)LSM 800 MATLSM 900 (with Airyscan 2)LSM 900 MATLSM 980 (with Airyscan 2)Scanning stage is required for all standsMotorozed objective nosepiece is recommendedDefinite Focus 2 is recommended for Axio Observer 7Software Requirements:ZEN blue 3.1 and aboveZEN blue 3.2 is required for overview image processing and detector parcentricity correction ZEN module Image Analysis is requiredZEN module Tile & Position is recommendedZEN module autofocus is recommended for software autofocusZEN module Intellesis is recommended for machine learning based image segmentationAdditional automation possible via the ZEN module Macro EnvironmentSeamless integration with ZEN Connect and Direct Processing modulesN o t a l l p r o d u c t s a r e a v a i l a b l e i n e v e r y c o u n t r y . U s e o f p r o d u c t s f o r m e d i c a l d i a g n o s t i c , t h e r a p e u t i c o r t r e a t m e n t p u r p o s e s m a y b e l i m i t e d b y l o c a l r e g u l a t i o n s . C o n t a c t y o u r l o c a l Z E I S S r e p r e s e n t a t i v e f o r m o r e i n f o r m a t i o n .E N _41_012_246 | C Z 03-2021 | D e s i g n , s c o p e o f d e l i v e r y , a n d t e c h n i c a l p r o g r e s s s u b j e c t t o c h a n g e w i t h o u t n o t i c e . | © C a r l Z e i s s M i c r o s c o p y G m b HCarl Zeiss Microscopy GmbH 07745 Jena, Germany ********************/microscopy。
蔡司显微镜AxioImager系列
蔡司显微镜AxioImager系列M i c r o s c o p y f r o m C a r l Z e i s sThe Future-oriented Imaging Platform for Biomedical Research and Materials ScienceAxio Imager 2Flexibility Meets ProgressThe best tools for biomedical sciences and for material applications. The new Axio Imager product generation will convince you with its outstanding perfor-mance, brilliant optics and maximum convenience in all applications.Axio Imager 2: a leap forward in quality and economyMore flexibility for greater performance: From observa-tion and documentation to complex analysis, the new Axio Imager product generation offers six stand configu-rations for biology and medicine and four for materials science. They can be adapted to precisely fit your ap-plication by means of individual system components. What they all have in common are pioneering features and technical innovations that ensure reliable and suc-cessful results.Excellent optics and homogeneous illumination in fluorescence, reflected-light and transmitted-lightEncoding: all important parameters are stored for later use ? Motorization for reproducible settingsPreconfigured stand configurations for a wide range of applicationsWhether you are working in the field of biology, medicine or materials science, with Axio Imager 2 you are ready to meet your growing needs.Flexibility for Life Sciences: Explore Life in All DimensionsThe diversity and complexity of tasks grow and so do the requirements placed on microscope technology as well. To be able to respond to changes quickly, systems are required that feature powerful components and can be equipped individually. With a range of applications that leaves all your options open, Axio Imager 2 precisely offers you the application that you need.this stand also allows Phase Contrast, darkfield, and DIC as well as reflected-light fluorescence at all magnifica-tions. When you are acquiring images with a camera, the color temperature can be kept constant across a wide intensity range using neutral density filters. Light and contrast settings can be stored in the light manager.Axio Imager.D2:more convenience in fluorescence applicationsFor more demanding applications, a motorized 6- or 10-position reflector turret is available for this stand. Like all Axio Imager 2 stands, this variant is also encoded. Axio Imager.D2 is particularly suited to applications such as Multi-color FISH involving a number of different fluo-rescent markers, for which corresponding single, double or multiple filter sets are used inquick succession. For optical sections, Axio Imager.D2 can also be equipped with ApoTome.Axio Imager.A2 LED:high-level basic configurationThe basic version of the new product family is the op-timum choice for examining specimens in transmitted-light brightfield. An LED light source for Fixed-Koehler illumination is a standard feature and guarantees a con-stant color temperature across the entire intensity range. If necessary, fluorescence or DIC can be upgraded at any time. The light manager stores the brightness settings for each magnification separately: an extremely convenient solution for sophisticated routine and research applica-tions.Axio Imager.A2: Koehler illumination for flexible contrasting techniquesUsers who perform more applications using transmitted- light allowing Koehler illumination are best served by the traditional halogen illumination offered by Axio Imager.A2. In addition to transmitted-light brightfield,Axio Imager 2 for Biology and Medicine Axio Imager.A2 LED Axio Imager.A2Axio Imager.D2Axio Imager.A2m:the flexible basic configurationThis basic stand for simple documentation, measurement, and analysis in materials microscopy can be used for ap-plications that do not require motorization. Typical areas of application include quality control, inspection or mea-surements. Thanks to the stand’s encoding, key micro-scope parameters can be acquired by the AxioVision soft-ware. The objective and total magnification, contrasting technique and brightness settings are stored and can be retrieved at any time.Axio Imager.D2m:epi-fluorescence and reflected-light brightfield easily combinedAxio Imager.D2m can be partially motorized and has been developed for more sophisticated requirements in material applications. With a motorized 6- or 10-position reflector turret and in combination with a motorizedswitching mirror for two lamps, it is also possible for fluorescent samples to be analyzed conveniently in either reflected-light brightfield or fluorescence.Axio Imager.M2m:operating comfort and reproducibilityThis is the high-performance stand for demanding qual-ity control, R&D and failure analysis applications per -formed in reflected-light. Its motorized focus drive makes Axio Imager.M2m ideal for complex applications such as topographic analyses of silver strip conductors on silicon solar cells. Thanks to the motorized reflected-light beam path, all light and contrast settings are repeatable. This instrument, which is equipped with a touchscreen for displaying the microscope status and controlling the motorized components, guarantees a highdegree of operating comfort and reproducibility. And the integrated user administration mode means that work can be man-aged simply and clearly – even in a multi-user environment.Axio Imager 2 for Materials ApplicationsAxio Imager.A2m Axio Imager.D2m Axio Imager.M2mIncreased Functionality for Materials Science:Stay Focused on Your ApplicationAxio Imager 2 is the microscope platform for all applications in the fields of materials analysis, materials development and quality control. It is available in four configura-tions –from encoded to fully motorized stands. Technology that provides the best possible support for your application and delivers quick and reliable results.Axio Imager.Z2m: the pinnacle in performance for the toughest challenges during continuous operation The Axio Imager.Z2m research platform can be fully motorized and perfectly equipped to cope with complex research and routine applications in which requirements change. As a microscope platform within the Particle Analyzer system, Axio Imager.Z2 can perform cleanli -ness analysis with extremely high precision. This stand is available with a motorized reflected-light beam path and also, as an option, with a motorized transmitted-light beam path. Reproducible illumination and contrast set-ting is possible thanks to the motorized features of this microscope. A motorizedfocus, which performs accurate focus movements even with heavy materials samples, guarantees maximum precision. It is also ideal for con-tinuous operation, e. g. for the automatic examination of large numbers of specimens.Axio Imager 2 for polarization microscopy:modularity means versatility from A to ZFrom Axio Imager.A2 to Axio Imager.Z2, each stand with-in the new product generation is suitable for polarized light microscopy. Axio Imager delivers reliable results with the best image quality in all polarized light microscopic analyses from mineral and structural characterizations to conoscopic analyses. Thanks to the modular system architecture, the instruments can be equipped flexibly and individually and can be easily expanded as your requirements grow. From mineralogy, crystallography, geology, and the glass and building materials industries to the fiber and textile industries, Axio Imager 2 can be used with polarization across all areas of application.Axio Imager.Z2m Axio Imager.A2 for polarization Axio Imager.Z2 for polarizationAxio Imager.M2p:geared for perfect pathologyThis variant of Axio Imager.M2 has been specifically tailored to the needs of pathologists. Equipped with an encoded nosepiece and a motorized focus drive, this stand has been designed to allow convenient, efficient, and ergonomic use of the microscope at a high sample throughput rate. When switching between overview and detail magnification, the parfocal adjustment function automatically sets the optimal focus position. The contrast manager, in conjunction with the motorized condenser, always adjusts the right contrast settingsfrom overview and higher magnification. This makes the evaluation pro-cess convincingly quick and easy.Axio Imager.M2: well-designed operating concept with touchscreen TFTPreconfigured with motorized transmitted-light beam path and focus drive and operated via a touch-sensitive TFT display or directly on the microscope, Axio Imager.M2 offers a substantially higher level of convenience and performance in every detail. The system is ideal for ana-lyzing and documenting specimens using different trans -mitted-light contrasting techniques with reproducible settings. The automatic switching between reflected- light and transmitted-light contrasting techniques as well as their compatibility enables Axio Imager.M2 to be used in applications at the boundary between the areas of bio-medicine and materials science. Typical experiments arefor example the combination of fluorescent markers, such as DAPI, FITC, and Rhodamine, with DIC in transmitted- light. Options for scanning stages allow full mosaic tiling and ApoT ome allows for structured illumination based on optical sectioning, thus expanding the flexibility of imaging modalities.Axio Imager.Z2:the imaging platform that meets highest demands Axio Imager.Z2, the flagship of the new product family, can rise to any challenge. It has been developed for con-tinuous operation in high-end research. The integrated user administration means that work can be managed simply and clearly –even by large groups. If you want to analyze large numbers of samples automatically over -night or achieve accurate focus movements over long periods using large scanning stages, Axio Imager.Z2 will be the perfect choice. It can be controlled using buttonsdirectly on the microscope or by means of the TFT display. The docking station allows you to control all motorized components remotely. And, when it comes to imaging, the motorization of the microscope guarantees definable and reproducible illumination and contrast settings. A particular highlight of Axio Imager.Z2 is the motorized DIC turret for combining transmitted-light DIC with fluo-rescence. This swings the DIC prism out of the beam path automatically as soon as you start the acquisition of a fluorescence image to ensure artifact free imaging.Axio Imager.M2p Axio Imager.M2Axio Imager.Z2* for imaging, reporting, interactive measurements, image analysis, and automation tasks:- AxioCam- AxioVision modules: Multidimensional Image Acquisition, Physiology module, Interactive Measurement, Image Analysis modules, Commander module, VBA moduleHippocampus (rat), Multichannel Fluorescence Objective: C-APOCHROMAT 10x/0,45 Trachea – brush border with microvilli (human)Objective: Plan-APOCHROMAT 20x/0,8Muscle (mouse)Objective: EC Plan-NEOFLUAR 40x/0,75Liquid-crystalline phase of [C 14mim]Br Polarization contrastEC EPIPLAN 10x/0.20 at 100 °C in a THMS600Linkam heating stageAnna Getsis and Anja-Verena Mudring, Faculty of Chemistry and Biochemistry, Solid-State Chemistry and Materials, Ruhr University Bochum, GermanyMaraging steel recast structure, nital etch with white,unetched areasDifferential Interference Contrast EC Epiplan-NEOFLUAR, 50x/0.80Sébastien Reymann, University of Applied Sciences,Materials Engineering Working Group, Aalen, GermanyPolycrystalline silicon solar cell BrightfieldEC EPIPLAN 100x/0.75Carl Zeiss MicroImaging GmbH, Light Microscopy,G?ttingen, Germany* for documentation, measuring tasks and analysis:- AxioCam- AxioVision modules: Interactive measurement, AutMess, Particle Analyzer, Graphite, Grains, Phase, Non-metallic inclusions, Linkam heating stage control。
蔡司 Axiolab 5 智能显微镜说明书
蔡司 Axiolab 5智能显微镜，让您的实验室日常工作更高效产品信息 版本 1.0Axiolab 5 适用于实验室中进行的常规显微镜检查工作。
其紧凑且符合人体工程学的设计可节省空间且易于操作。
Axiolab 5 是研究团队中的得力助手。
与 Axiocam 208 color 组合，充分发挥智能显微镜功能的优势——体验全新的显微数码成像方式。
只需聚焦样品并按下一个按钮，便可轻松获得清晰的真彩图像。
数字图像与您从目镜中观察的效果一致，所有细节和细微色差均清晰可辨。
另外，Axiolab 5 还会自动向图像添加正确的比例尺信息。
这一系列操作可单机完成，无需使用计算机或任何其他软件。
使用 Axiolab 5 既节省时间又节约成本，而且还可节约实验室的宝贵空间。
显微数码成像从未如此简单。
智能显微镜，让您的实验室日常工作更高效› 简介› 优势› 应用› 系统› 技术参数›售后服务更简单、 更智能、 更高度整合提升实验室日常工作的效率找到感兴趣区域后，只需按下显微镜主机两侧的拍照按钮，即可获得图像，操作简单便捷。
Axiolab 5 易于操作且符合人体工程学的设计理念，使其成为实验室日常工作的好帮手。
您甚至无需移动手的位置即可控制显微镜及其连接的相机。
智能显微镜系统将自动调节参数，以当时所显示的样品状况进行精确地记录，并获得包含细节信息的真彩图像。
同时也会自动添加正确的比例尺信息。
您无需再额外购买计算机或软件。
智能显微镜可以让您的工作更高效，始终专注于样品。
更经济、更可靠Axiolab 5 对您而言既节省成本又节能。
例如，启用 Eco 模式后，Axiolab 5 将在闲置 15 分钟后自动进入待机模式。
这一操作不仅节能，而且还延长了光源的使用寿命。
与传统照明系统相比，LED 的使用寿命更长。
在透射光下，全新的高性能白光 LED 让您能够观察到原色样品的图像。
即使是细微色差，仍清晰可辨。
在荧光应用中，具有不同波长的内置 LED 相比于传统汞灯使用更方便且更安全。
Zen Starter简易操作手册——【蔡司体视显微镜】
• 点击停止，完成拼图拍摄
单个视野
重叠区域
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ZEN 2 core拍照测量报告流程 点击可生成数据表
• 根据样品的情况选择拍摄方式拍 摄图像（单幅，景深或拼图）
蔡司Zen Starter简易操作手册
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目录
ZEN 2 core软件介绍……………………………………3
➢ 单幅图像拍摄…………………………………………7 ➢ 手动景深扩展拍摄……………………………………8 ➢ 手动拼图拍摄…………………………………………9
➢ 典型工作流：拍照测量生成报告………………10 ➢ 典型工作流：拍照测量保存图像………………11 ➢ 典型工作流：制作标尺信息…………………………12 ➢ 典型工作流：相机阴影校正…………………………13 ➢ 典型工作流：相机水平校准…………………………14 ➢ 典型工作流：修改测量标注属性流程………………15
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启动软件
• 双击桌面上的软件图标ZEN 2 core v2.5启动软件 • 进入软件后点击自由测试
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ZEN 2 core操作界面
添加工作台
添加工具
预览窗口
图库
工作台
工具
图像显示调节
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ZEN 2 core添加工作台
• 点击添加工作台，选择工作台类型，然后选择添加所需工作台，如 2D图像拍摄
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ZEN 2 core添加工具
• 点击添加工具，选择工具类型，然后选择添加所需工具，如 直方图
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ZEN 2 core单幅图像拍摄
ZEISS Axio Imager Vario 大规模物理观察设备说明书
ZEISS Axio Imager VarioExamine Large Specimens – Automated and Compatible with Clean RoomsProduct Information Version 1.0Axio Imager Vario brings out your research, development, and quality assurance specimens so that they are larger than life, regardless of whether you are working with tiny MEMS sensors or XXL wafers. On top of this, a maximum specimen size of 300 mm × 300 mm and an impressive maximum specimen height of 254 mm make sure that you will be able to analyze large specimens nondestructively. And all this supported by the stability provided by a column design. Examine your wafers in your clean room – Axio Imager Vario is DIN EN ISO 14644-1-certified and meets the requirements corresponding to clean room class ISO 5. Finally, a motorized Z-axis drive and the Hardware Auto Focus system ensure that you will always be able to automatically bring low-contrast, reflective specimens into perfect focus so that you will always get optimum results.Bring Large Specimens Into Focus – Quickly and Reproducibly› In Brief › The Advantages › The Applications › The System› Technology and Details › ServiceAnimationZEISS Axio Imager Vario: Simpler. More intelligent. More integrated.Stretch Your LimitsSelect from two manual and one motorized columns and take advantage of a maximum specimen size of 300 mm × 300 mm and an impressive maxi-mum specimen height of 254 mm. Whether dealing with heavy specimens or working in combination with the LSM 700 laser scanning microscope – the sturdy column design provides reliable stability and prevents vibrations. In addition, you can expand your possibilities even further by selecting from various stages for reflected and transmitted light applications, as well as from various specimen holders.In Focus at all TimesIf you need to examine the surface of reflective,low-contrast specimens, you can simply equipAxio Imager Vario with the fast and efficientHardware Auto Focus system. This systemguarantees a high precision of up to 0.3 timesthe objective’s depth of field, and is well suitedto both reflected light and transmitted light appli-cations. The sensor detects changes in the focusposition, any deviations are compensated auto-matically. This means that even large specimenswill remain perfectly focused while being movedin the X- and Y-axis directions.Certified for Clean Room WorkWafer and photomask inspections are subjectto extremely strict requirements concerningcleanliness. This is why Axio Imager Vario isDIN EN ISO 14644-1-certified and, togetherwith a clean room kit, meets the requirementscorresponding to clean room class ISO 5. More-over, extensive accessories, such as a seven-posi-tion objective turret with a guard designed toprotect against foreign particles and a sneezingguard, ensure that your specimens will alwaysremain perfectly clean. All of this, of course, withyour components retaining all of their functio-nality and performance.› In Brief› The Advantages› The Applications› The System› Technology and Details › ServiceYour Insight into the Technology Behind ItExaminations in the fields of research and industrial production (e.g. surface examinations of reflective, low-contrast specimens such as metallographic speci-mens and polished or textured wafers) require a fast focusing system that ensures high precision levels of max. 0.3 times the objective’s depth of field. This requirement can be easily met by combining your Axio Imager Vario with the Hardware Auto Focus system to benefit from fast and accurate focusing across a wide capture range of up to 12,000 µm. The Hardware Auto Focus system is designed to work with reflected light and transmitted light microscopy in brightfield, darkfield, polarized light, DIC, and oblique illumination applications.How it WorksThe objective guides the structured light produced by an LED in the Auto Focus system’s sensor module onto the specimen, with the specimen’s surface reflecting it back. During this process, Auto Focus permanently analyses the properties of the reflected LED light and derives the appropriate control signals for the focus drive, to bring the surface into focus. The Auto Focus system comes with three different modes corresponding to different specimen characteristics (reflective/partially reflective/diffuse) and with three different precision levels (precision/balance/speed). The Auto Focus sensor detects changes and deviations in the focus position. These are then automatically compensated by the direct access of Auto Focuscontroller to the microscope’s Z-drive.How the Hardware Auto Focus system works: 1) LED 2) Sensor module 3) Sensor 4) Beam splitter 5) O bjective 6) Specimen› In Brief› The Advantages › The Applications › The System› Technology and Details › ServiceYour Insight into the Technology Behind ItSemiconductor device fabrication work and wafer inspection operations are performed in clean rooms in order to protect components from impurities that could have an impact on their operation. Accordingly, the use of clean rooms is accompanied by especially strict requirements concerning air quality. Clean rooms are categorized into various classes as per DIN EN ISO 14644-1, which relies on the amount and size of particles per cubic meter as defining criteria. Axio Imager Vario is certified for use in clean rooms in accordance with DIN EN ISO 14644-1 and, when used in conjunction with a clean room kit, meets the requirements of the ISO 5 clean room class, which is the one most frequently used; it corresponds to class 100 in the original FED STD 209E (1992) standard. This clean room kit comes with a special seven-position objective turret, as well as with guards providing protection against particles and sneezing. All components are delivered indouble packaging, properly cleaned and airlock-ready.Clean Room Classes as per DIN EN ISO 14644-1› In Brief› The Advantages › The Applications › The System› Technology and Details › ServiceExpand Your PossibilitiesThe small manual column allows for specimen sizes of up to 200 mm × 200 mm in the X- andY-axis plane and specimen heights of up to 254 mm.The large manual column allows for specimensizes of up to 300 mm × 300 mm in the X- andY-axis plane and specimen heights of up to 254mm. This track stand post is suitable for use withLSM 700.The motorized column allows for specimen sizesof up to 300 mm × 300 mm in the X- and Y-axisplane and specimen heights of up to 254 mm,and features three-button controls as per indus-trial standards. This track stand post is suitable foruse with LSM 700.› In Brief› The Advantages› The Applications› The System› Technology and Details › ServiceExpand Your PossibilitiesZEISS Axio Imager Vario and ZEISS LSM 700Combining Axio Imager Vario and LSM 700 opens up a new world of possibilities. In fact, specimens that need to be analyzed at high resolutions with no contact almost seem to have been made spe-cifically for this combination. Extremely fine lateral fragments with sizes as small as approx. 120 nm (scribe line structure/width) can be optically resolved in great detail. And with LSM 700, you can detect the smallest surface defects (with a size of only a few nanometers) with extreme precision so that you can pinpoint their exact location. By using Axio Imager Vario in combination with LSM 700, you can obtain laser scribe topographies and thin-film solar cell surface topologies. You will be able to measure laser scribes and determine surface roughnesses with much greater precision. Another typical application is being able to obtain topographies of the silver paste in crystalline silicon solar cells in order to evaluate the quality of the corresponding print screen.› In Brief› The Advantages › The Applications › The System› Technology and Details › ServiceTailored Precisely to Your Applications› The Advantages› The Applications› The System› Technology and Details› Service200 µmZEISS Axio Imager Vario at WorkReflected light, C-DIC, EC Epiplan-APOCHROMAT 50x/0.95Silver finger on polycrystalline silicon solar cell; EC Epiplan-APOCHROMAT 20x/0.60Silver finger: 3-D reconstruction on monocrystalline silicon solar cell; EC Epiplan-NEOFLUAR 20x/0.50Monocrystalline Silicon Solar CellLaser edge isolation scribe: laser-textured edge isolation scribe on monocrystalline silicon solar cell; EC Epiplan-APOCHROMAT 20x/0.60› In Brief › The Advantages › The Applications › The System› Technology and Details › ServiceZEISS Axio Imager Vario at WorkReflected light, darkfield; EC Epiplan-NEOFLUAR 10x/0.25Reflected light, darkfield; EC Epiplan-NEOFLUAR 50x/0.953-D reconstruction: a Z-series was captured with the AxioVision Topography module and shown as a 3-D reconstruction.Print ScreenRotated 3-D reconstruction› In Brief › The Advantages › The Applications › The System› Technology and Details › ServiceZEISS Axio Imager Vario at WorkReflected light, polarized light; EC Epiplan-NEOFLUAR 50x/0.80CdTe thin-film solar cell: laser texture on thin-film solar cell in TCO coating on glass; reflected light, polarized light; EC Epi-plan-NEOFLUAR 50x/0.80Silicon thin-film solar cell: surface of a thin-film solar cell; re-flected light, polarized light; EC Epiplan-APOCHROMAT 50x/0.95Thin-film Solar CellSilicon thin-film solar cell: surface of a thin-film solar cell; reflected light, polarized light with lambda plate; EC Epiplan-APOCHROMAT 50x/0.95› In Brief › The Advantages › The Applications › The System› Technology and Details › ServiceZEISS Axio Imager Vario at WorkReflected light, darkfield; EC Epiplan-APOCHROMAT 10x/0.30Wafer with debris: reflected light, C-DIC, EC Epiplan-APOCHROMAT 50x/0.95Pattern defects; reflected light, brightfield, EC Epiplan-APOCHROMAT 50x/0.95WaferReticle pattern: transmitted light, brightfield, EC Epiplan-APOCHROMAT 10x/0.30› In Brief › The Advantages › The Applications › The System› Technology and Details › ServiceZEISS Axio Imager Vario at WorkTransmitted light, brightfield, EC Epiplan-APOCHROMAT 10x/0.30Hot stuck pixel: bright spot on black background caused by blue subpixel stuck in the “ON” state.Dead stuck pixel: dark spot on white background caused by red subpixel stuck in the “OFF” state.TFT DisplayDebris on LCD: can result in dark sports; can be distinguished from dead subpixels under a microscope.› In Brief › The Advantages › The Applications › The System› Technology and Details › Service5214365 Software• AxioVision, AxioVision LE Recommended AxioVision modules:• MosaiX (image acquisition, scanning stage)• Graphite, Grains, Multiphase, NMI, ParticleA nalyzer, Comparative Diagrams, OnlineM easurement, Shuttle & Find (image analysis)6 Accessories • Hardware Auto Focus • Linear sensor• Stages: XY stage, reflected light/transmitted light, 200 × 200 RXY stage, reflected light, 300 × 300 R Scanning stage, 200 × 300 STEP Scanning stage, 300 × 300 STEP3 Illumination • 12 V 100 W halogen • 100 W HBO • microLED4 CamerasRecommended cameras:• AxioCam HRc • AxioCam MRc5• AxioCam MRc • AxioCam ICc 51 Microscopes• Axio Imager.A2 Vario (manual, coded)• Axio Imager.Z2 Vario (capable of being fully motorized)• Axio Imager.Z2 Vario (without turret focus)2 Objectives• Reflected light: EC EPIPLAN, EC Epiplan- NEOFLUAR, EC Epiplan-APOCHROMAT • Transmitted light: N-ACHROPLAN, EC Plan- NEOFLUAR, Plan-APOCHROMAT, C-APOCHROMAT, FLUAR• Special purpose: LD EPIPLAN, LD EC Epiplan-NEOFLUARZEISS Axio Imager Vario: Your Flexible Choice of Components› In Brief › The Advantages › The Applications › The System› Technology and Details › ServiceZEISS Axio Imager.A2 Vario: System Overview› In Brief› The Advantages› The Applications› The System› Technology and Details› ServiceZEISS Axio Imager.Z2 Vario: System Overview› In Brief› The Advantages› The Applications› The System› Technology and Details› ServiceTechnical Specifications› In Brief › The Advantages › The Applications › The System› Technology and Details › ServiceTechnical Specifications› The Advantages› The Applications› The System› Technology and Details› ServiceTechnical Specifications› In Brief› The Advantages› The Applications› The System› Technology and Details› ServiceTechnical Specifications› The Advantages› The Applications› The System› Technology and Details› ServiceTechnical Specifications› In Brief› The Advantages› The Applications› The System› Technology and Details› ServiceBecause the ZEISS microscope system is one of your most important tools, we make sure it is always ready to perform. What’s more, we’ll see to it that you are employing all the options that get the best from your microscope. You can choose from a range of service products, each delivered by highly qualified ZEISS specialists who will support you long beyond the purchase of your system. Our aim is to enable you to experience those special moments that inspire your work.Repair. Maintain. Optimize.Attain maximum uptime with your microscope. A ZEISS Protect Service Agreement lets you budget for operating costs, all the while reducing costly downtime and achieving the best results through the improved performance of your system. Choose from service agreements designed to give you a range of options and control levels. We’ll work with you to select the service program that addresses your system needs and usage requirements, in line with your organization’s standard practices.Our service on-demand also brings you distinct advantages. ZEISS service staff will analyze issues at hand and resolve it – whether using remote maintenance software or working on site. Enhance Your Microscope System.Your ZEISS microscope system is designed for a variety of updates; open interfaces allow you to maintain a high technological level at all times. As a result you’ll work more efficiently now, while extending the productive lifetime of your microscope as new update possibilities come on stream.Please note that our service products are always being adjusted to meet market needs and maybe be subject to change.Profit from the optimized performance of your microscope system with services from ZEISS – now and for years to come.Count on Service in the True Sense of the Word>> /microservice› In Brief › The Advantages › The Applications › The System› Technology and Details › ServiceThe moment "I think" becomes "I know".This is the moment we work for.MADE By ZEISS› In Brief › The Advantages › The Applications › The System› Technology and Details › ServiceE N _42_011_037 | C Z 07-2013 | D e s i g n , s c o p e o f d e l i v e r y a n d t e c h n i c a l p r o g r e s s s u b j e c t t o c h a n g e w i t h o u t n o t i c e . | © C a r l Z e i s s M i c r o s c o p y G m b HCarl Zeiss Microscopy GmbH 07745 Jena, Deutschland Materials********************/axioimagervario。
蔡司 Axiovert 5 数码款细胞成像系统说明书
/axiovert-digital蔡司Axiovert 5数码款您的一体式细胞成像系统质臻至简，配备蔡司AI 技术100 µm从自动驾驶和智能家居，到为智能手机加密的面部识别系统，人工智能（AI ）已为我们的日常生活提供了诸多便利。
是时候将人工智能也带入您的细胞实验室了。
Axiovert 5数码款采用人工智能和自动功能，助您轻松完成日常工作。
它能让您享受更高效的工作流程，并获得可重复性更高的结果。
即使面对繁多的工作任务，您也可以轻松应对。
Axiovert 5数码款的人工智能经过预先训练，汲取了蔡司丰富的经验：我们已导入大量数据集，使其尤为可靠。
只需按下一个按钮，便可以获得实时结果。
您的一体式细胞成像系统单击此处观看本段视频› 简介› 优势› 应用› 系统› 技术参数› 售后服务更简单、更智能、更高度集成开箱即用畅想一体式显微镜系统的诸多优势。
从常规的科学工作到基础研究，从相差到多通道荧光成像，使用Axiovert 5数码款，即使是新手也能采集到出色的图像。
打开系统，设置和调整已然就绪，您仅需专注于样品，无需进行繁琐操作，便可立即投入工作。
您也不必担心细胞在密闭培养箱内的状态，可以随时关注它们的变化。
Axiovert 5 数码款将可重复性和数据质量提升至新水平。
您可以始终依靠仪器的出色性能，得到可供发表的图像。
简单易用Axiovert 5数码款的设计支持相应的系统操作，是您多用户环境的理想之选。
其一体式成像系统具有直观的操作理念，只需点击一下拍照按钮即可实现以下功能：• 多达5个通道的图像采集（包括多通道 成像）• AI 细胞计数和融合度工作流，采集并实时分析图像• 视频记录Axiovert 5数码款将可靠的光学质量和简单易用巧妙结合。
节省时间，让人工智能为您效力借助Axiovert 5数码款，轻松节省您的宝贵时间，而这些时间可能对细胞的活力至关重要。
无论是设置系统和采集参数、培训新同事、采集图像，还是从图像到产生结果，在各个环节上您都可以节省时间。