Promentum-ATCA-4310

合集下载

一种氧化葡萄糖酸杆菌中基因抑制的方法[发明专利]

一种氧化葡萄糖酸杆菌中基因抑制的方法[发明专利]

专利名称:一种氧化葡萄糖酸杆菌中基因抑制的方法专利类型:发明专利
发明人:周景文,秦志杰,刘立,陈坚,曾伟主,堵国成
申请号:CN202010978371.2
申请日:20200917
公开号:CN112094843A
公开日:
20201218
专利内容由知识产权出版社提供
摘要:本发明公开了一种氧化葡萄糖酸杆菌中基因抑制的方法,属于基因工程和生物工程技术领域。

本发明将相应靶基因crRNA在氧化葡萄糖酸杆菌中进行表达。

通过验证对质粒上mCherry荧光表达的抑制作用,表明重组氧化葡萄糖酸杆菌有基因抑制的作用,并且通过对氧化葡萄糖酸杆菌基因组中的其他基因进行抑制,也达到了类似的抑制效果,进一步证明了此系统对基因组上基因的抑制功能。

为在氧化葡萄糖酸杆菌中抑制目的基因提供了一种新方法,能够快速、有效抑制目的基因的表达,提高了对氧化葡萄糖酸杆菌的基因编辑效率。

申请人:江南大学
地址:214000 江苏省无锡市滨湖区蠡湖大道1800号
国籍:CN
代理机构:哈尔滨市阳光惠远知识产权代理有限公司
代理人:林娟
更多信息请下载全文后查看。

PROMEGA逆转录试剂盒说明书

PROMEGA逆转录试剂盒说明书

Reverse Transcription System
INSTRUCTIONS FOR USE OF PRODUCT A3500.
1. Description..........................................................................................................1 2. Product Components and Storage Conditions ............................................2 3. Reverse Transcription Protocol.......................................................................2
Printed in USA. Revised 3/09 Part# TB099 Page 1
2.
Product Components and Storage Conditions
Size 100 reactions Cat. # A3500
Product Reverse Transcription System
3.A. Reverse Transcri magnesium concentration may be optimized for any given sequence to achieve better yields. **Final concentration of reaction components: 5mM MgCl2; 1X Reverse Transcription Buffer (10mM Tris-HCl [pH 9.0 at 25°C]; 50mM KCl; 0.1% Triton® X-100); 1mM each dNTP; 1u/μl Recombinant RNasin® Ribonuclease Inhibitor; 15u/μg AMV Reverse Transcriptase (High Conc.); 0.5μg Oligo(dT)15 Primer or Random Primers per microgram RNA; 50ng/μl 1.2kb Kanamycin Positive Control RNA, poly(A)+ mRNA or total RNA.

木材蓝变生物控制菌康氏木霉胞外几丁质酶的纯化及特性

木材蓝变生物控制菌康氏木霉胞外几丁质酶的纯化及特性

德 国 、英 国和 日本 等 国家 的许 多学者 相 继 开展 了这
方 面的研 究 ,取 得一 些研 究成 果 。经过 研究 有 些生
物 防治菌 株 已应 用到 防蓝 变 的生 产实 践 中 ,如蓝 变 菌 的 白化 菌株 在加 拿 大 已经商 业 化生 产 ,用 于 防治
木材蓝 变 【 ;美 国林 产 品实验 室 ( P )开 发研 制 1 F L 的 2种 含 有木 霉属 真 菌繁殖 体 的 菌丸 ,现 已应 用 于
酶活 测 定 采 用 DNS法 L l ,酶 活 力 单 位 ( U) 定义 :1酶 活 力单 位 U 定 义 为 每 分 钟 在 3 ℃ 下 每 7 小 时水 解 几 丁质 产 生 1 mo 还 原 糖 所 需 的酶 蛋 白 / l  ̄
量。 1 4 蛋 白 质 浓 度 的 测 定 .
滤液 经 过 硫 酸 铵 分 级 沉 淀 、Q H 强 阴离 子 交 换 柱 层 析 、P e y S p ao e疏 水 层 析 、C S p aoe弱 阳 离 子 交 -P h n l h rs 。 c M- e h r s
换 柱 层 析 、P e y S paoe疏 水 柱 层 析 后 得 到 凝 胶 电 泳 ( D -A E) 谱 带 单 一 的 几 丁 质 酶 ,其 分 子 量 为 hn l hrs — c S SP G 3k a 6 D ,最 适 温 度 为 4 1 ,最 适 p 为 4 0 0 2 H . 。多 种 金 属 阳 离 子 对 该 酶 具 有 影 响 ,C 2 a 、F z 酶 活力 有 比较 明 显 e 对
遍存 在并 有 重要 经 济意义 的生 防益 菌 。木霉 ( r Ti —
c o ema s p ) 作 为 一 种 重 要 的 植 病 生 防 因 子 hdr p .

植物病害诊断试剂盒

植物病害诊断试剂盒

植物病害诊断试剂盒美国阿格迪agdia 公司是全球最大的植物病害诊断试剂生产商,产品品种最多,可检测项目多达200多个。

包装规格最全,不同的包装规格适合不同规模的实验室。

从中您一定能发现适合您使用的产品。

选购试剂说明,请仔细阅读。

1,kit, 订货号PSAxxxxx/xxxx 或PSPxxxxx/xxxx 为完整的试剂盒包装,包括样品提取缓冲液、包被好抗体的微孔板(可拆分)、酶标记物、稀释液、缓冲液、底物发色剂、阳性质控(如果应该供应)。

特别注明Indirect ELISA 方法的kit 包括未包被的微孔板及联接用的抗体,所含有的其他组分同上。

2, Reagent Set, 订货号SRAxxxxx/xxxx ,XRAxxxxx/xxxx或SRPxxxxx/xxxx 只含有未包被的微孔板、包被需要的抗体或联接用的抗体、酶标记物。

其他试剂如样品提取缓冲液、稀释液、缓冲液、底物发色剂、质控物需另外订购或自己配制。

我公司销售原厂的上述试剂,详见目录。

3, Bacterial Reagent Set, 订货号BRAxxxxx/xxxx 只含有未包被的微孔板、包被需要的抗体或联接用的抗体、酶标记物。

其他试剂如样品提取缓冲液、稀释液、缓冲液、底物发色剂、质控物需另外订购或自己配制。

我公司销售原厂的上述试剂,详见目录。

4, Bacterial ID订货号BIDxxxxx/xxxx 为完整的试剂盒包装,包括样品提取缓冲液、包被好抗体的微孔板(可拆分)、酶标记物、稀释液、缓冲液、底物发色剂、质控(如果应该供应)。

用于鉴定培养基中或有病症植物提取液中的细菌。

操作简便快速。

5, PS A或SR A中的A代表碱性磷酸酶标记;PS P或SR P中的P代表过氧化物酶标记。

6, Immunostrip test, 为检试纸条,操作简单,几分钟内得到结果,非常适合于现场检测。

该试条必须与相应的样品提取缓冲液配套使用。

实验室需要单独购买该样品提取缓冲液,详见目录。

宝赛菌株和质粒目录-2014

宝赛菌株和质粒目录-2014

pET-3a(+)pET-3c-sumo pET-3d(+)pET-11a(+)pET-12a(+)pET-15b(+)pET-16b(+)pET-17b(+)pET-19b(+)pET-20b(+)pET-21a(+)pET-21b(+)pET-21d(+)pET-22b(+)pET-23a(+)pET-23b(+)pET-24a(+)pET-25b(+)pET-26b(+)pET-27b(+)pET-28a(+)pET-28b(+)pET-28c(+)pET-28a(+)-sumo pET-28a(+)-GFP pET-28a(+)-SMT3 pET-29a(+)pET-30a(+)pET-30c(+)pET-31b(+)pET-32a(+)pET-32a(+)pelB pET32a-LC3pET-32a(+)LicpET-35b(+)pET-38b(+)pET-39b(+)pET-40b(+)pET-41a(+)pET-42a(+)pET-43.1a(+)pET-44a(+)pET-49b(+)pET-50b(+)pET-52b(+)pMCSF1pMSCF3pRSFDuet1 pCDFduet1pColA duetE.coli EPI300Ecoli MC1061BL21BL21goldBL21(DE3)BL21(DE3)plysSBL21(DE3)GoldplysS BL21AIBL21SIBL21codonplusRIPL BL21codonplus(DE3) BL21TrxB(DE3)BL21(DE3)RDBL21Star(DE3)BL21RPBL21RILRosetta(DE3)Rosetta(DE5)Rosetta Blue(DE3) Rosetta(DE3)plysS Rosetta2(DE3)Rosetta2(DE3)plysS Rosetta-gami(DE3) Origami(DE3)Origami2(DE3) OrigamiB(DE3)Origami(DE3)plysS Rosetta-gami 2(DE3)placI Rosetta-gami 2(DE3)pLysS Rosetta-gami B(DE3)pLysS BLR(DE3)Novablue(DE3)B834(DE3)JM83AD494(DE3)HM174(DE3)HM174(DE3)plysSC41(DE3)C41(DE3)plysSC43(DE3)Turner(DE3)Turner(DE3)plysSJM101JM105JM109JM109(DE3)DH5aλpirK12MG1655XL1blueXL10 goldTop10Top10F’BM25.8BW23473SG1117TurboT1TG1TB1M15ER2566C2566ER2529ER2738HB2151S-17SM10JF1125BJ5183HB101K802C600JM110TH1AM1W3110DH10bacY1089Y1090Antarctic Express Antarctic Express(DE3) Antarctic Express(DE3)RP Antarctic Express(DE3)RILDH5αJM110TOP10XL2-Blue MRF’Mach1 T1OmniMAX2 T1 Phage-Resistant Cells EZ10DH10BSUREpubs 520DB3.1pCold IpCold IIpColdIIIpCold-sumopCold-TFpPin point xa1pPin point xa2pPin point xa3pPin point CATpMAL-c2xpMAL-p2xpMAL-p5xpMAL-c5xpMAL-c4xpMAL-p4xpMAL-p5epTWIN1pTWIN2pLLP-OmpApLLP-STIIpMBP-PpMBP-CpET-TrxpET-HispTrc-CKSpET-DsbApET-MBPpET32M3CpGEX-4T-3pGEX-5x-1pGEX-6p-1pGEX4T-1/Gst-His-HA pGEX3XpGEX-1λT-6His-GST-PP pGEX2tkpkk223-3pkk232-8pBV220pBV221pBV222pRsetApRsetBpRsetCpRsetB td Tomato pBBRMCS pBBRMCS2 pBBRMCS3 pBBRMCS4 pBBRMCS5pTrcHisApTrcHisBpTrcHisCpBADHis ApBADHis BpBADHis CpBADMycHisA pQE2pQE9pQE30lacIqpQE30pQE31pQE32pQE40pQE60pQE70pQE80L pQETrisystem pEZZ18pSE380pWHM3pQBI63pTrc99A pTrcMECTpTrcpTYB1pTYB2pTYB11pTYB12pCYB1pBAD18pBAD24pBAD33pBAD43pBAD24-GFP pDest15-N-throbin pDest22pDest32pED-Trx-pp-air pED-pppED-DsbA-pp-airpED-GST-pp-airpCWori+pITG TrxpT7tspTT5pALEXpUC18pUC19-GFPpUC19pUC18-p43pUC118pGEM3ZF+pEGM-7ZF(+)pEGM-11ZF(+)pKT100pME6032SuperCosmidpBR322pACYC184pACYC177pBluescript II SK(+) pBluescript SK(+) pBluescript II KS(+)pG-KJE8pGro7pKJE7pG-Tf2pTf16pEC86pET-28a-fabppETcoco-1酵母杂交系统pACTpACT-MyoDpBIND-IdpG5 lucpCMV-BDpCMV-ADpBD-p53pBD-NF−κBpAD-SV40TpAD-TRAFpFR-lucpGBKT7pGADT7pCL1pGBKT7-53pGADT7-TpGBKT7-LampACT2 ADY187AH109pSospMyrpSos MAFBpHis2pHisSi-1p53bluepGAG424pLaczip53hispGAD53mpBridgeY2H Gold Yeast strain Y187pINDpOPRSVIpOPI3CATpCMVLacIpSwitchpGene v5-His BpcDNA4/TO/Myc-His A pcDNA4/TO/Myc-His B pcDNA4/TO/Myc-His C pcDNA4/TO/Myc-His/LacZ pcDNA6/TRpTet-OnpTet-OffpTRE2pTRE2 hygropTK-hygpRevTet-OnpRevTet-OffpRevTREpTet on advancedpTRE TightpTRE Tight Luciferase pCMV-Tet3GpTRE3GpTRE3G-LucpNI v2pNG v2pNN v2pHD v2pTALETF v2 (NN) pTALETF v2 (NG) pTALETF v2 (NI) pTALETF v2 (HD) pTALEN v2 (NI) pTALEN v2 (NG)pTALEN v2 (NN)pTALEN v2 (HD)pX335pX260pX320pShuttle-CMVpShuttlepAdTrack-CMVpAdTrackpAdEasy-1BJ5183AdEasy-1pAd/BLOCK-iT-DEST RNAi Gateway Vector pDC315pBHGloxdelE13crepShuttle-IRES-hrGFP2pAAV-MCSpAAV-RC2pAAV-RCpHelperpAAV-LacZpAAV-IRES-hrGFPpCMV-MCSRNAi-Ready pSIREN-RetroQRNAi-Ready pSIREN-RetroQ-DsRed-Express RNAi-Ready pSIREN-RetroQ-ZsGreen pSilencer5.1-U6-RetropSilencer5.1-H1-RetropRetroX-IRES-ZsGreen1pRetroX-IRES-DsRed ExpresspLNCX2pQCXIHplvx-RNA1plvx-RNA2pMSCVpuropSuperpSuper retro GFP-neo pSupeior puropBabe puropCMV-Gag-polpCMV-VSV-GpCL AmphopSuper retro puropLVX-shRNA1pLVX-shRNA2pSicoRpSicoR pGK puro pLentilox 3.7pLKO.1 puroPLKO.1pLKO.3GpTet-PLKO-puro pPRIME-TET-GFP-FF3 pPRIME-TREX-GFP-FF3 pSIH1-H1-CoGFPpGIPZ emptypGIPZ controlpTRIPZ emptypTRIPZ controlpLVX-DsRed-Monomer-N1 pLVX-AcGFP1-N1pLVX-IRES-ZsGreen1 pLVX-IRES-td Tomato pLVX-IRES-mCherry pLVX-IRES-neopLVX-IRES-puropLVX-puropLVX-Tight-PuropLVX-Tet-On-AdvancedpLVX-TRE 3GpLVX-Tet 3Gplvx-i-TDpLenti4 TO/V5 DESTpLenti6 V5 DESTpLenti6-UbC-V5-DESTpCDH-CMV-MCS-EF1-copGFP pCDH-CMV-MCS-EF1-puro pCDH-CMV-MCS-EF1-GFP+Puro pCDH-EF1-MCS-T2A-Puro pWPXLFUGWpLVTHpLVTHMpLp1pLp2pLp VSV GpCgpVpVSV GpCMV-dR8.91pCMV-VSV-GpSPAX2pMD2.GpMDLg pRREpRSV-revpCMV-VSV-GpLentG-KOSMTetO-FUW-OSKMFUW-M2rtTAFUW-tetO-hOCT4FUW-tetO-hSOX2FUW-tetO-hKLF4FUW-tetO-hMYCpCDF1-MCS2-EF1-copGFPpFP93LeGO-iC2pLOX-CW-CREpLOX-CWBmi1pLOX-TERT-iresTK pLOX-Ttag-iresTK pGensil-1Stbl3Stbl4Stbl4PiggyBac Dual promoter Super PiggyBac Transposase pGAS-TA-LucpSTAT3-TA-LucpISRE-TA-LucpTA-LucpIκB-EGFPpNFAT-TA-Luc pCaspase3-sensorpAP1(PMA)-TA-Luc pCRE-LucpGRE-LucpHSE-Lucp53-LucpAP-1-LucpNF-κB-Lucp38-betap38-бp38-γπp-JNKK2pCDNA3.1-flag-TRAP2 pCDNA3.1-flag-TAK1HA-ASK1pCDNA3-myc-MEKK3pCMV-MDMZpEGFP-N1-raf通路pSRE-LucpGL4.10pGL4.13pGL4.19pGL4.26pGL4.27pGL4.29pGL4.30pGL4.75TOPFlashFOPFlash SuperTopFlashpE2F-TA-LucpSilencer1.0pSilencer 2.1-U6 hygro pSilencer3.0-H1 pSilencer 3.1-H1 hygro pSilencer 3.1-H1 neo pSilencer 4.1-CMV neo pSilencer 4.1-CMV puro pMIR-REPORT Luciferase pSV-β-galacosidase psiCHECK-2pmir GLOpGenesil-1pmR mCherrypmiRZip anti-microRNA pRNATin-H1.2/Neo pRNATin-H1.2/Hygro pRNATin-H1.2/Retro pRNA-H1.1/Adeno pYES2pYES2-EGFPpYES2-KanpYES2-flagpYES3/CTpYES6/CTpYES2 NTApYES2 NTBpYES2 NTCpFA6a-GFP(S65T)-His3MX6_1x pFA6a-GFPS65T-KanMX6 pESC-LeupESC-TrppESC-HispESC-UrapRS316pRS316HApRS426pRS426galpRS41HpRS41H-启动子pYCP211pYIP5pYRP7pYX212pYIP211pADH2PACT2-ADpDR195p416GFDpYEplac112pYEplac195Ycp22lac-EGFPYcplac33pAUR123pUG6pUG35pSH65pSH63pSH47 INVSc1S288cW303-1ABY4741BY4742FY837YPH499YS58YM4271 pPIC9pPIC9kpPIC9khis pPIC9kmut pPICZalphaA pPICZalphaB pPICZalphaC pPICZA pPICZB pPICZC pGAPZalphaA pGAPZalphaB pGAPZalphaC pGAPZA pGAPZB pGAPZC pPIC.5k pPIC3.5pAO815 pPink HCpPink LCpPink HC alphapMET αBpMET αCpPIC ZαGBpPIC ZαFCpPIC ZαDpHIC-PILA503ZαGApFLD-catp334BFD15pink1pink2pink3pink4SMD1168SMD1168HSMD1163KM71KM71HGS115GS190GS200JC308JC220pENTR 1ApENTR3CpDONR221pDONR ZeopcDNA6.2-GWEmGFP-miR negative pLenti6/TRDB3.1pYD1EBY100pDisplay植物载体pcambia35s-EGFP pcambia2301-101 pcambia35s-EYFP pcambia35s-ECFPpTCK303pBI101pBI221-GFPpBI121pBI121-GFPpcambia1300-221Super1300pcambia1300GFP pcambia 1200pcambia1201pcambia12811Zpcambia1300pcambia1301pcambia1302pcambia1303pcambia1304pcambia1305.1pcambia1305.2pcambia1380pcambia1381xcpcambia1381xapcambia1381pcambia1381xbpcambia1381Zpcambia1390pcambia1391pcambia1391Zpcambia1391xapcambia1391xcpcambia1391xbpcambia2200pcambia2300pcambia2301pcambia0380pcambia0390pcambia2201pcambia1291ZATCC15834 发根农杆菌LBA9402pKANNIBALpHANNIBALpGreen029pSPYCE(M)pSPYCE(MR)pspYCEMpspYCEMRpspYNE173pspYNE173RpSAT4 nVenus-CpSAT1 CCFP-NpSAT1 CCFP-CpPZp-RCS2-BarpSAT6 nCerulean C(A+)p416GFDpDF15枯草芽孢杆菌表达系统pMUTIN4pMUTIN4GFPpCSN44pMA5pMA5MCSpMA-H3pMA09-JpMA09-HpHY300pHY300plkpXMJ19pIL253pZL507pDG1363pSG1154pHT304pHCMC04 pHCMC05pAD43-25pMA5pMA5MCSpGFP315pGFP22pHT01pHT43BCL10501A75WB600BS168WB600WB800WB800NpHIS1525pK18mobSacB巨大芽孢菌 WH320巨大芽孢菌YYB pHT1谷氨酸棒杆菌ATCC13032其他酵母表达系统pKLAC1GG499pSEP1pSEP2pSEP3粟酒酵母SP01粟酒酵母SPQ01T载体系列pCXSNpCUXNpXDGpXDR细胞通路载体p38-betap38-бp38-γπp-JNKK2pCDNA3.1-flag-TRAP2 pCDNA3.1-flag-TAK1HA-ASK1pCDNA3-myc-MEKK3 pCMV-MDMZpEGFP-N1-raf通路JM109 MEKK1 fl pcDNA3-HA-TAB1ERK (WT)Pgex4t1-GST-IKB pSicheck2pQCXINpDONR221Top10 pROSEJM109 MEKK1 fl ERK (WT)pBabc-puroHA A8F1HAcdc37pcMV flag MEKK1 pBARK1pBD67其他表达系统pOS7001pHGF9050pIJ8860pRK2013pVLT33pJLA 502pIL253PSM-402pAP-B03pECX99EpXJM19pCS2-HApDG148pMK3S17-lamda pir pMG36ePYCTpNZ8048MG1363NZ9000pBIG2R-HPH2pAN7-1pAN8-1pKC1139哺乳动物真核表达质粒pSR-GFP/NeopVAX1pBudCE4.1pSFV1pCEP4pUB6/V5 His ApUB6/V5 His BpUB6/V5 His CpUB6/V5 His lacZpCMV5pCMV-Tag 2 BpCMV-Tag 5BpSecTag2 ApNTAP-BpBK-CMVpTracer CMV2pSG5pCI-neopCIpSIpCMV-mycpCMV-HApIRESpIRES neopIRES neo3pIRES hyg3pIRES puro3pIRES2-EGFPpIRES-hrGFP-1a pIRES-Dsred-Express pBI CMV1pFLAG CMV2pVitro2-neo-mcs pCAGGSpWHEREpBC1pcDNA4T/0pcDNA4/V5-His A pcDNA6TRpCDNA3.0-GFPpCDNA3.0pcDNA3 flagpcDNA3 EGFPpcDNA3.1HApcDNA3.1MycpcDNA 3.1-c-His/myc pcDNA3.1(+)/Myc HisB pcDNA3.1(zeo)+ pcDNA3.1/GSpcDNA3.1(-)/myc-His A pcDNA3.1-V5-HisA pcDNA3.1-CT-GFP pcDNA3mito RFP pcDNA4/HisMax B pcDNA6-Myc/His B pIE1pIEX-bac3pCDNA6 myc His C pfastbacIpFastBacIIIpFastBacI-Gus pFastBacHT ApFastBacHT B pFastBacHT C pFastBacHT-CAT pFastBacDualpFastBac-c-His-TEV pFastBac-N-GST-TEV pBlueBacHis2 A pBlueBacHis2 B pBlueBacHis2 CpIEXBac-1pIEXBac-c-EGFP-3 pIEXBac-c-EGFP-1 pIEXBac-c-EGFP-4pVL1392pVL1393pVL1392-XyIE control vector pCo-blastpAc5.1apAc5.1bpAc5.1 V5-His BpMT/V5 HispMT-Bip-V5-HisApEF1/myc-His BpEF1/V5 HisApEF4/V5 HisApEF6/Myc-HisCpMIB v5-His BpIZT/V5-Hissf9细胞High Five细胞DH10BacpFBDMpUCDMpBADZ-His6CreDH10MultiBac pDsRed2-N1 pDsRed2-C1 pDsRED-Monomer-N1 pDsred2-4T-1 pDsred2-32a pscherry1pmCherry-N1 pmCherry-C1pRFP-N3pEYFP-N1pEYFP-C1pEGFP-N1pEGFP-C1pEGFP-N3pEGFP-C3pECFP-N1pECFP-C1pEGFP-1pAcGFP1-1pDsRed2-1 pZsYellow-N1 pAmCyan-N1 pLEGFP-N1 pLEGFP-C1 pDsRED2-ER pDsRED2-Mito pDsRED2-Nuc pDsRED2-Peroxi pDsred-Express C1 pEYFP-GolgipEYFP-MempEYFP-ERpEYFP actinpEYFP tubpEGFP-ActinpECFP-ERpECFP-Mito pPAmCherry-Tubulin mRFP-ERDkeima fluorescent protein mAmetrinepDsRed-Monomer pEBFP-N1pEBFP-C1pEBFP-C2pAcGFP1-1pGreen0029pSicor EGFPpCDNA3.1(-)-MRFP pGL3-basicpGL3-controlpGL3-enhancerpGL3-promoterPGL3-SV40PGL3-CM 转DH6αpRL-TKpRL-CMVpRL-SV40pGluc-basicpSIM5pSIM6pKD3pKD4pKD20pKD46PCP20基因野生型P53myc-hTERTSV40 Large TTaqklentaqPfuKODT4 DNA ligaseT4 PNKTEVSUMOSUMO protease 2pP-αSUMO3GST-3CLYZ-pPICZα(溶菌酶表达毕赤酵母载体)Vaccinia viru Topoisomerase Imouse RITNFTGFLysozymeM-MLVM-MLV(-)CreCCDB-TsfGFP大肠杆菌DNA聚合酶I大片段(klenow)大肠杆菌DNA聚合酶I普通微生物菌种红发夫酵母(自己分离)酿酒酵母S288c(自己分离)葡萄牙假丝酵母(自己分离)多形汉逊酵母 NRRL Y-11170白色念珠菌ATCC 10231酿酒酵母 S.c197管囊酵母(自己分离)原毛黄孢平革菌 ATCC 34540热带假丝酵母 NRRL Y-12968马其顿假丝酵母CICC 1765巴斯德假丝酵母 NRRL Y1603三角酵母(自己分离)南极假丝酵母NRRL Y-7954 Ash bya gossypii NRRL Y-1056酿酒酵母 CGMCC2.1多形汉逊酵母 NRRL Y-11170出芽短梗霉 CICC40333黑曲霉(自己分离)简青霉CGMCC3.4402热带假丝酵母 CICC 1272树干毕赤酵母 NRRL Y-11545光滑假丝酵母NRRL Y-65长娄德酵母CGMCC2.1589季也蒙毕赤酵母CGMCC2.1801克鲁维酵母K.aestuarii Y-5370出芽短梗霉 NRRL Y-2311-2出芽短梗霉(自己分离)出芽短梗霉 ATCC 62921美季假丝酵母CGMCC2.1919安格斯毕赤酵母 NRRL Y-2214德巴利汉逊酵母NRRL Y-2021解脂亚罗酵母 CICC1441白色假丝酵母CGMCC 2.538卡尔斯博厄酵母 CICC1746赭色掷孢酵母CGMCC2.2113赭色掷孢酵母NRRL Y-5483简青霉(自己分离)乳酸克鲁维酵母CICC1773近平滑假丝酵母 CGMCC2.1786构巢曲霉ATCC38163博伊丁假丝酵母 NRRL Y-2332-2荚膜毕赤酵母 NRRL Y-1842博伊丁假丝酵母 NRRL Y-2332安格斯毕赤酵母 NCYC 495绿色木霉 NRRL3652大毛霉 NRR3635黑曲霉 NRR566米根霉(自己分离)嗜热脂肪地衣芽孢杆菌 NRRL B-1102嗜热脂肪地衣芽孢杆菌 NRRL B-1172嗜热脂肪地衣芽孢杆菌 NRRL B-14316嗜热脂肪地衣芽孢杆菌 NRRL B-14318解淀粉芽孢杆菌 CGMCC1.1099地衣芽孢杆菌 ATCC33632巨大芽孢杆菌NRRL B-14308巨大芽孢杆菌CGMCC 1.223蜡状芽孢杆菌 NRRL B-3711嗜碱芽孢杆菌ATCC BAA-125蜡状芽孢杆菌 CICC10040地衣芽孢杆菌 ATCC 14580巨大芽孢杆菌 ATCC12872芽孢杆菌 NRRL B-11291紫红红球菌 NICMB 11216紫红红球菌DSM11097紫红红球菌DSM44541赤红红球菌 CGMCC4.1187红串红球菌 CGMCC 1.2362红串红球菌 DSM 43297紫红红球菌 CGMCC 1.2348紫红红球菌 DSM6263紫红红球菌 DSM46022紫红红球菌 DSM363紫红红球菌DSM43002嗜吡啶红球菌JCM10940恶臭假单胞菌 NRRL-18668荧光假单胞菌 DSM7155荧光假单胞菌CGMCC 1.758洋葱假单胞菌(自己分离)绿叶假单胞菌 CICC10216洋葱假单胞菌(自己分离)野油菜黄单胞菌 DSM3586丁香假单胞菌 ATCC BAA-978粪产碱杆菌CGMCC1.767粪产碱杆菌ATCC8750反硝化产碱杆菌N4谷氨酸棒杆菌ATCC13032金黄节杆菌ATCC BAA-1386藤黄节杆菌 CGMCC1.1525柠檬黄节杆菌CGMCC1.1893植物乳杆菌 NCIMB8826鲍曼不动杆菌 CICC22934鲍曼不动杆菌CICC22934醋酸钙不动杆菌NCIMB9871糖多孢菌 NRRL 2338四联球菌 NRRL B-108肺炎链球菌ATCC 49619肺炎克雷伯氏菌(自己分离)敏捷食酸菌72w睾丸酮丛毛单胞菌 ATCC55746运动发酵单胞菌 ZM4嗜水气单胞菌2(自己分离)嗜水气单胞菌1(自己分离)睾丸酮丛毛单胞菌 ACCC10192睾丸酮丛毛单胞菌 ATCC 55744聚团斯塔普氏菌JCM 20685耐辐射甲烷菌JCM2831四联球菌NRRL B-108金黄色葡萄球菌 ATCC 6538金黄色葡萄球菌ATCC29213臭鼻克雷氏菌CGMCC1.1734哈维氏弧菌ATCC33842河流弧菌CGMCC1.1608副溶血弧菌(自己分离)嗜热假诺卡氏菌CGMCC10280沉积物希瓦氏菌 DSM17055脆壁希瓦氏菌 NCIMB 400农杆菌 NRRL B-11291铅青链霉菌阿维链霉菌 NRRL 8165茎瘤固氮根瘤菌 DSM 5975放射根瘤菌ATCC 33970粪肠球菌ATCC29212粪肠球菌 ATCC 7080洋葱伯克霍尔德菌ACCC 10506根瘤菌ATCC BAA-1182结核分枝杆菌 JCM13017 Prosthecochloris vibrioformis DSM263 Oceanicola granulosus ATCC863质控标准菌株人伤寒沙门菌(自己分离)猪伤寒沙门菌(自己分离)大肠杆菌ETEC大肠杆菌EIEC大肠杆菌EHEC大肠杆菌EPEC苏云金芽孢杆菌ATCC10792枸橼酸杆菌CMCC48001小肠耶尔森菌ATCC23715黄色微球菌ATCC58166宋内志贺ATCC9290阴沟肠杆菌ATCC45031溶藻胶弧菌ATCC17749枯草芽孢杆菌ATCC63501白色葡萄球菌8032鲍曼不动杆菌ATCC19606肺炎克雷伯氏菌ATCC13883甲型副伤寒ATCC9250拟志弧菌ATCC33847河弧菌NCTC11218大肠杆菌ATCC25922创伤弧菌ATCC27526铜绿假单胞菌ATCC27853金黄色葡萄球菌ATCC25923鼠伤寒杆菌ATCC14028单增李斯特菌CMCC54002变形杆菌CMCC49027白色念珠菌ATCC10231副溶血弧菌ATCC17082福氏志贺菌ATCC12022鲍氏志贺菌ATCC9207蜡样芽孢杆菌ATCC11778阪琦肠杆菌ATCC51329表皮葡萄球菌ATCC12228粪链球菌CMCC3200马红球菌ATCC6939大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达宿主菌大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌报告基因质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌表达质粒大肠杆菌克隆质粒大肠杆菌克隆质粒大肠杆菌克隆质粒大肠杆菌克隆质粒大肠杆菌克隆质粒大肠杆菌克隆质粒大肠杆菌克隆质粒大肠杆菌克隆质粒大肠杆菌克隆质粒大肠杆菌克隆质粒大肠杆菌克隆质粒大肠杆菌克隆质粒分子伴侣分子伴侣分子伴侣分子伴侣分子伴侣大肠杆菌克隆质粒大肠杆菌表达质粒大肠杆菌表达质粒哺乳动物双杂交系统哺乳动物双杂交系统哺乳动物双杂交系统哺乳动物双杂交系统哺乳动物双杂交系统哺乳动物双杂交系统哺乳动物双杂交系统哺乳动物双杂交系统哺乳动物双杂交系统哺乳动物双杂交系统哺乳动物双杂交系统哺乳动物双杂交系统酿酒酵母双杂交系统酿酒酵母双杂交系统酿酒酵母双杂交系统酿酒酵母双杂交系统酿酒酵母双杂交系统酿酒酵母双杂交系统酿酒酵母双杂交系统酿酒酵母双杂交系统酿酒酵母双杂交系统酿酒酵母双杂交系统酿酒酵母双杂交系统酿酒酵母双杂交系统酿酒酵母单杂交系统酿酒酵母单杂交系统酿酒酵母单杂交系统酿酒酵母单杂交系统酿酒酵母单杂交系统酿酒酵母单杂交系统酿酒酵母单杂交系统酿酒酵母三杂交系统酿酒酵母三杂交系统酿酒酵母三杂交系统蜕皮激素诱导表达系统蜕皮激素诱导表达系统LacSwith II哺乳动物诱导表达系统LacSwith II哺乳动物诱导表达系统LacSwith II哺乳动物诱导表达系统GeneSwith 哺乳动物诱导系统GeneSwith 哺乳动物诱导系统四环素诱导系统四环素诱导系统四环素诱导系统四环素诱导系统四环素诱导系统四环素诱导系统四环素诱导系统四环素诱导系统四环素诱导系统四环素诱导系统四环素诱导系统四环素诱导系统四环素诱导系统四环素诱导系统四环素诱导系统四环素诱导系统四环素诱导系统四环素诱导系统四环素诱导系统Tale ToolBox kitTale ToolBox kitTale ToolBox kitTale ToolBox kitTale ToolBox kitTale ToolBox kitTale ToolBox kitTale ToolBox kitTale ToolBox kitTale ToolBox kitTale ToolBox kit Tale ToolBox kit CAS9系统CAS9系统CAS9系统腺病毒系统腺病毒系统腺病毒系统腺病毒系统腺病毒系统腺病毒系统腺病毒系统腺病毒系统腺病毒系统腺病毒系统腺病毒系统腺相关病毒系统腺相关病毒系统腺相关病毒系统腺相关病毒系统腺相关病毒系统腺相关病毒系统腺相关病毒系统逆病毒系统逆病毒系统逆病毒系统逆病毒系统逆病毒系统逆病毒系统逆病毒系统逆病毒系统逆病毒系统逆病毒系统逆病毒系统逆病毒系统逆病毒系统逆病毒系统逆病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统慢病毒系统PiggyBac转座子系统PiggyBac转座子系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统信号通路报告系统RNAi系统RNAi系统RNAi系统RNAi系统RNAi系统RNAi系统RNAi系统RNAi系统RNAi系统RNAi系统RNAi系统RNAi系统RNAi系统RNAi系统RNAi系统RNAi系统RNAi系统RNAi系统酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒酿酒酵母表达质粒。

碧云天MG-132 Proteasome抑制剂产品说明书

碧云天MG-132 Proteasome抑制剂产品说明书

碧云天生物技术/Beyotime Biotechnology订货热线:400-168-3301或800-8283301订货e-mail:******************技术咨询:*****************碧云天网站微信公众号网址:MG-132 (Proteasome抑制剂)产品编号产品名称包装S1748-1mg MG-132 (Proteasome抑制剂) 20mg/ml×0.05mlS1748-5mg MG-132 (Proteasome抑制剂) 5mgS1748-25mg MG-132 (Proteasome抑制剂) 25mg产品简介:MG-132也写作MG132,也称Z-LLL-CHO、Z-Leu-Leu-Leu-CHO、Z-Leu-Leu-Leu-al或Carbobenzoxy-L-leucyl-L-leucyl-L-leuc inal,是一种常用的Proteasome抑制剂,即蛋白酶体抑制剂。

MG-132可以通透细胞,选择性抑制Proteasome,Ki=4nM。

可以激活JNK1并抑制NF-κB的激活(IC50=3µM)。

在10µM时可以诱导PC12细胞产生neurite outgrowth。

MG-132分子量为475.62,分子式为C26H41N3O5,CAS Number:133407-82-6。

本产品纯度大于99%。

本MG-132为进口分装,其中20mg/ml包装产品用DMSO配制,共0.05ml。

5mg和25mg包装为粉末装。

包装清单:产品编号产品名称包装S1748-1mg MG-132 (Proteasome抑制剂) 20mg/ml×0.05mlS1748-5mg MG-132 (Proteasome抑制剂) 5mgS1748-25mg MG-132 (Proteasome抑制剂) 25mg—说明书1份保存条件:-20ºC避光保存,半年有效。

英国普隆防腐剂有效物

英国普隆防腐剂有效物

PROMEX 产品列表Actives(单体)PROMEXTM BIT 1,2-Benzisothiazolin-3-onePROMEXTM BNP 2-Bromo-2-nitropropane-1,3-diol (Bronopol)PROMEXTM CMT 5-Chloro-2-methyl-4-isothiazolin-3-one (CMIT)+2-Methyl-4-isothiazolin-3-one (MIT)PROMEXTM CTL ChlorothalonilPROMEXTM CZ Methyl 1H-benzimidazol-2-ylcarbamate (Carbendazim) PROMEXTM DBNPA 2,2-Dibromo-3-nitrilopropionamidePROMEXTM DN N-(3,4-dichlorophenyl)-N,N-dimethylurea (Diuron)PROMEXTM DZ 3,5-Dimethyl-2H-1,3,5-thiaziazine-2-thionePROMEXTM FS Ethylenedioxy-1,2-dimethanolPROMEXTM IPBC 3-Iodo-2-propynylbutyl carbamatePROMEXTM MIT 2-Methyl-4-isothiazolinonePROMEXTM OIT 2-n-Octyl-4-isothiazolin-3-onePROMEXTM PHMB Poly(imino imido carbonylimino hexamethylene Biguanide) PROMEXTM TZ 1,3,5-Tris-(2-hydroxyethyl)-1,3,5-hexahydrotriazine (Triazine)Eco-friendly VOC free Dispersions(零VOC水分散体)PROMEXTM 10D Aqueous dispersion of 10% 1,2-Benzisothiazolin-3-one (BIT) PROMEXTM 20D Aqueous dispersion of 20% 1,2-Benzisothiazolin-3-one (BIT) PROMEXTM 33D Aqueous dispersion of 33% 1,2-Benzisothiazolin-3-one (BIT) PROMEXTM 45D Low viscosity aqueous dispersion of45% 1,2-Benzisothiazolin-3-one (BIT) (frost stable to -30℃)PROMEXTM BBD Aqueous dispersion of 1,2-Benzisothiazolin-3-one +2-Bromo-2-nitropropane-1,3-diol (BNP)PROMEXTM BMD Aqueous dispersion of 2.5% 1,2-Benzisothiazolin-3-one (BIT) +2.5% 2-Methyl-4-isothiazolin-3-one (MIT)PROMEXTM BMBD Aqueous dispersion of 2.5% 1,2-Benzisothiazolin-3-one (BIT) +2.5% 2-Methyl-4-isothiazolin-3-one (MIT) + 2-Bromo-2-nitropropane-1,3-diol (BNP)PROMEXTM BCD Aqueous dispersion of 1,2-Benzisothiazolin-3-one (BIT) +5-Chloro-2-methyl-4-isothiazolin-3-one (CMIT) + 8.0% 2-Methyl-4-isothiazolin-3-one (MIT)PROMEXTM BID Aqueous dispersion of 1,2-Benzisothiazolin-3-one (BIT) +3-Iodo-2-propynylbutyl carbamate (IPBC)PROMEXTM CTL40D Aqueous dispersion of 40% ChlorothalonilPROMEXTM FPD Aqueous dispersion of 2-n-Octyl-4-isothiazolin-3-one(OIT), Diuron and CarbendazimPROMEXTM IGZ Aqueous dispersion of N’-tert-butyl-N-cyclopropyl-6-(methylthio)-1,3,5-triazine-2,4-diamine(Cybutryne) + Zinc pyrithione + 2-n-Octyl-4-isothiazolin-3-one (OIT)Solutions(溶液,其中绿色的都是零VOC的产品)PROMEXTM Na5S Eco-friendly VOC free aqueous solutions of 5% 1,2-Benzisothiazolin-3-one (BIT) sodium saltPROMEXTM Na20S Glycolic solution of 20% 1,2-Benzisothiazolin-3-one (BIT) sodium salt New!PROMEXTM Na20TS Eco-friendly VOC free solution of 20% 1,2-Benzisothiazolin-3-one (BIT) sodium saltPROMEXTM K10A Eco-friendly VOC free aqueous solution of 10% 1,2-Benzisothiazolin-3-one (BIT) potassium saltPROMEXTM K10G Eco-friendly VOC free aqueous solution of 10% 1,2-Benzisothiazolin-3-one (BIT) potassiumsalt (frost stable to -5℃)PROMEXTM K20S Glycolic solution of 20% 1,2-Benzisothiazolin-3-one (BIT) potassium saltPROMEXTM BMG Eco-friendly VOC free aqueous solution of 2.5% 1,2-Benzisothiazolin-3-one (BIT) +2.5% 2-Methyl-4-isothiazolin-3-one (MIT)PROMEXTM BTZ Aqueous solution of 1,2-Benzisothiazolin-3-one (BIT) +1,3,5-Tris-(2-hydroxyethyl)-1,3,5-hexahydrotriazine (Triazine)PROMEXTM BHS Glycolic solution of 1,2-Benzisothiazolin-3-one (BIT) + Ethylenedioxy-1,2-dimethanolPROMEXTM BNC Eco-friendly VOC free aqueous solution of 2-Bromo-2-nitropropane-1,3-diol (BNP) +5-Chloro-2-methyl-4-isothiazolin-3-one (CMIT) + 2-Methyl-4-isothiazolin-3-one (MIT)PROMEXTM BNP30 Glycolic solution of 30% 2-Bromo-2-nitropropane-1,3-diol (BNP) PROMEXTM CHS3/CHS4 Glycolic solution of 5-Chloro-2-methyl-4-isothiazolin-3-one (CMIT) +2-Methyl-4-isothiazolin-3-one (MIT) + Ethylenedioxy-1,2-dimethanolPROMEXTM CMT1.4 Aqueous solution of 1.4% 5-Chloro-2-methyl-4-isothiazolin-3-one (CMIT) +2-Methyl-4-isothiazolin-3-one (MIT)PROMEXTM CMT1.5/2.5SF Aqueous solution of 1.5%/2.5% 5-Chloro-2-methyl-4-isothiazolin-3-one (CMIT) +2-Methyl-4-isothiazolin-3-one (MIT)PROMEXTM COS Optimised aqueous solution of Octyl-4-isothiazolin-3-one (OIT) +5-Chloro-2-methyl-4-isothiazolin-3-one (CMIT) + 2-Methyl-4-isothiazolin-3-one (MIT) PROMEXTM DFG Glycolic solution of 2-n-Octyl-4-isothiazolin-3-one (OIT) +Methyl 1H-benzimidazol-2-ylcarbamate (Carbendazim)PROMEXTM ED33S Aqueous solution of 33% Ethylenedioxy-1,2-dimethanol PROMEXTM FS Aqueous solution of 50% Ethylenedioxy-1,2-dimethanol PROMEXTM IPBC20S Glycolic solution of 20% 3-Iodo-2-propynyl butyl carbamate PROMEXTM IPBC30S Glycolic solution of 30% 3-Iodo-2-propynyl butyl carbamate PROMEXTM IPO Glycolic solution of 3-Iodo-2-propynyl butyl carbamate (IPBC) +2-n-Octyl-4-isothiazolin-3-one (OIT)PROMEXTM MIT10 Aqueous solution of 10% 2-Methyl-4-isothiazolin-3-one PROMEXTM OIT14 Glycolic solution of 14% 2-n-Octyl-4-isothiazolin-3-one PROMEXTM OIT45 Glycolic solution of 45% 2-n-Octyl-4-isothiazolin-3-one PROMEXTM PH20S Aqueous solution of 20% Poly(imino imido carbonylimino hexaMethylene Biguanide) (PHMB)PROMEXTM PCB20S Glycolic solution of 20% DBNPAPROMEXTM MIT10 Aqueous solution of 1,3,5-Tris-(2-hydroxyethyl)-1,3,5-hexahydrotriazine (Triazine)。

普洛麦格TnT

普洛麦格TnT

中 文 说 明 书适用产品目录号:L1170, L1171, L2080 和 L20812020 版 CTM045原英文技术手册TM045TnT ®Quick Coupled Transcription/Translation Systems G9711, G9712 and G9713普洛麦格(北京)生物技术有限公司Promega (Beijing) Biotech Co., Ltd 地址:北京市东城区北三环东路36号环球贸易中心B 座907-909电话:************网址: 技术支持电话:800 810 8133(座机拨打),400 810 8133(手机拨打)技术支持邮箱:*************************TM0452020制作1TnT ®Quick Coupled Transcription/Translation Systems 1. 产品描述 (2)2. 产品组成和储存条件 (6)3. 一般注意事项 (7)3.A. DNA 模板注意事项 (7)3.B. 创建无核糖核酸酶的环境 (8)3.C. 裂解物的处理 (8)4. 翻译流程 (8)4.A. 使用质粒DNA 进行TnT ®Quick Coupled Transcription/Translation Reaction 的一般操作流程 (9)4.B. 使用PCR 产生的DNA 进行TnT ®Quick Coupled Transcription/Translation Reaction 的一般操作流程 (10)4.C. 说明 (11)5. 使用萤光素酶的阳性对照翻译反应 (12)5.A. 放射性萤光素酶对照反应 (12)5.B. 非放射性萤光素酶对照反应 (12)6. 使用犬胰腺微粒体膜的共翻译加工 (13)6.A. 使用微粒体膜进行翻译的一般操作流程 (13)7. 翻译后分析 (14)7.A. 蛋白印迹分析 (15)7.B. 放射性标记物掺入百分比的测定 (16)7.C. 放射性标记翻译产物的变性凝胶分析 (17)7.D. 使用FluoroTect™Green Lys 体外翻译标记系统标记的翻译产物的变性凝胶分析 (18)7.E. 使用Transcend™非放射性翻译检测系统标记翻译产物的变性凝胶分析 (19)8. 阳性对照萤光素酶检测 (20)8.A. 使用发光检测仪 (20)8.B 使用闪烁计数器 (20)9. 疑难解答 (21)10. 参考文献 (22)11. 附录 (24)11.A. 缓冲液和溶液的组成 (24)11.B. 萤光素酶SP6 / T7对照DNA (25)11.C. 相关产品 (27)12. 变更概要................................................................................................................................................................30所有技术文献的英文原版均可在/ protocols 获得。

乳腺癌细胞株整理

乳腺癌细胞株整理
加入10%的胎牛血清
具有CD44+/hi/CD24-
/低癌症干细胞标记
LY2
浸润性导管癌
胸腔积液
DMEM培养基,90%;优质胎牛血清,10%。气相:37C,5%CO2
ER(+);PR(-);TP53(+/-)
MCF7
乳腺腺癌细胞
浸润性导管癌
乳腺,从转移位点(胸水)处获得/上皮细胞样,
MEM培养基中加入0.01mg/ml人重组胰岛素和10%胎牛血清。
细胞角蛋白19
HCC202
腺癌
乳房,乳腺
RPMI-1640,90%;优质胎牛血清,10%。气相:37°C,5%CO2
ER(-);PR(-);HER2(+);
TP53-
HCC2157
腺癌
乳房,乳腺
RPMI-1640,90%;优质胎牛血清,10%。气相:37°C,5%CO2
ER(-);PR(-);TP53+
贴壁生长,三阴,转移性乳腺导管腺癌
MDA-MB-436
乳腺腺癌细胞
浸润性导管癌
乳腺腺癌胸水转移灶/
有多核成分的多形细胞
L-15培养添加10ug/ml胰岛素和16ug/ml谷胱甘肽,90%;优质胎牛血清,10%。气相:空气,100%。温度:37°C。
ER(-);PR(-);TP53-
MDA-MB-453
DMEM培养基,90%;优质胎牛血清,10%。气相:37C,5%CO2
ER(+);PR(+);P53(+/-Wt表达WNT7B表达胰岛素样生长因子结合蛋白
(IGFBP)BP-2;BP-4;BP-
5;TNF-a可以抑制MCF-7细胞的生长;
MCF-10A

Seppro

Seppro

Seppro®IgY 14 LC10 ColumnCatalog Number SEP040Storage Temperature 2–8 °CTECHNICAL BULLETINProduct DescriptionThe Seppro®IgY 14 Liquid Chromatography 10 (LC10) Column is based on avian antibody (IgY)-antigen interactions and optimized buffers for sample loading, washing, eluting, and column regeneration. The column is specifically designed to remove fourteen highly abundant proteins from human fluids such as serum or plasma. The following proteins are depleted in a single step:Albumin IgGα1-Antitrypsin IgAIgM TransferrinHaptoglobin α2-Macroglobulin Fibrinogen Complement C3α1-Acid Glycoprotein (Orosomucoid)HDL (Apolipoproteins A-I and A-II)LDL (mainly Apolipoprotein B)The targeted highly abundant proteins are simultaneously removed by the immobilized specific IgYs when crude biological samples are passed through the column.Selective immunodepletion provides an enriched pool of low abundance proteins for downstream proteomic analyses. Specific removal of these fourteen highly abundant proteins depletes ∼95% of the total protein mass from human serum or plasma. The low abundance proteins in the flow-through fractions can then be studied. Removal of highly abundant proteins enables improved resolution and dynamic range for one dimensional electrophoresis (1DGE), two dimensional (2DGE) electrophoresis, and liquid chromatography/ mass spectrometry (LC/MS). The collected flow-through fractions may need to be concentrated dependent upon the downstream application.Characteristics of the IgY 14 LC10 ColumnSize: 12.7 ×79.0 mm(10 ml bed volume)Capacity: 10.0 mg of total protein or ∼143 µl of human plasma based on an average protein concentration of 70 mg protein/ml.Note: If the protein concentration of the sample is unknown and the total serum protein level is potentially elevated, a reduction of the serum load to 100 µl is recommended for initial study to avoid potential abundant protein bleed through.Total protein mass removal: ∼95%Targeted depletion efficiency: 95% (average) Maximum operation pressure: 350 psi (21 bars) Antibody-modified resin only withstands 100 psi Flow rate: 0.5–2.0 ml/minuteOperating temperature: 18–25 °CShipping Buffer: 1×Dilution Buffer with 0.02% sodium azideColumn body materials: Polycarbonate column cylinder, Polyethylene frit, Tefzel®caps, Buna-N-rubberO-rings, Delrin®nut fittings, ETFE ferrules, andPTFE PFA tubing.Usage: Column may be used 100 times.2ComponentsSeppro IgY 14 LC10 Column 1 each (Catalog Number S5074)10×Dilution Buffer 3 ×200 ml Tris-Buffered Saline (TBS) -100 mMTris-HCl with 1.5 M NaCl, pH 7.4(Catalog Number S4199)10×Stripping Buffer 3 ×200 ml1 M Glycine, pH 2.5(Catalog Number S4324)10×Neutralization Buffer 3 ×80 ml1 M Tris-HCl, pH 8.0(Catalog Number S4449)Corning®Spin-X®Centrifuge Tube Filters 1 pack0.45 µm, pack of 100(Catalog Number CLS8163)Precautions and DisclaimerThis product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.Specimen collection needs to utilize universal precautions.Preparation InstructionsPreparation of 1×concentration buffers -Separately dilute the three 10×buffers (Dilution, Stripping, and Neutralization Buffers) 10-fold with water. If precipitation occurs in the 10×buffers, allow the bottle to warm to room temperature and mix untilcompletely dissolved prior to use. Do not dilute all of the 10×Neutralization Buffer, save a volume of the 10×neutralization buffer for neutralization of eluted bound proteins if analysis of bound proteins is desired. Sample Preparation -It is not recommended to load unfiltered serum directly onto the column. Human serum samples should be diluted 5-fold with 1×Dilution Buffer. Samples may contain particulate materials, which can be removed with a 0.45 µm spin filter, centrifuge for 1 minute at 9,000 ×g.Storage/StabilityStore the column at 2–8 °C. After use, equilibrate the column with 1×Dilution Buffer containing 0.02% sodium azide and store the column at 2–8 °C with the end-caps tightly sealed. Do Not Freeze the column.ProcedureNote: Always use the three 1×buffers as the mobile phases for the LC procedure. Adjust the LC procedure appropriately for the instrumentation being used. Do not expose the column to solvents other than the three 1×buffers. Do not expose the column to organic solvents (like alcohols, acetonitrile, etc.), strong oxidizers, acids, or reducing agents and other protein denaturing agents (urea).1.Set up the three 1×buffers as the only mobilephases.2.Purge lines with the three 1×buffers and run the1×Dilution Buffer at 2 ml/minute without a columnto check the system back pressure.Note: The maximum operation pressure includesthe pressure introduced by the column and thesystem backpressure from the instrument. Usually, the pressure introduced by the column is less than50 psi. It is important to first check the systembackpressure of the instrument before using thiscolumn. If the system backpressure is more than300 psi, use tubing with a larger I.D.or change the flow-cell to tubing with a larger I.D. to reduce thesystem backpressure.3.Attach the column to chromatography instrument(see Appendix) and equilibrate it with1×DilutionBuffer for 20 minutes at a flow rate of 2.0 ml/minute to obtain a flat baseline.4.Set up a LC timetable (see Table 1) and run twomethod blanks by injecting 1,250µl of1×DilutionBuffer.Note: Adjust LC timetable based on instrumentation available, if necessary.5.Inject 1,250 µl of the diluted and filtered serum (seeSample Preparation), start with a flow rate of0.5 ml/minute for 30 minutes, wash the column at aflow rate of 2.0 ml/minute for 5 minutes, collectflow-through fraction, and store collected fractionsat –70 °C if not analyzed immediately.Note: Due to high salt concentration in the1×Dilution Buffer, buffer exchange of the flow-through fractions to a volatile buffer (for example,ammonium bicarbonate) is recommended prior tolyophilization.36.Elute bound proteins from the column with1×Stripping Buffer at a flow rate of 2.0 ml/minutefor 15 minutes and neutralize the eluted fractionswith 0.1×fraction volume of 10×Neutralizing Buffer.Note: Do not expose the column to the 1×Stripping Buffer for more than 20 minutes.7.Neutralize the column with 1×Neutralizing Buffer ata flow rate of 2.0 ml/minute for 10minutes.8.Re-equilibrate the column with 1×Dilution Buffer foran additional 10 minutes at a flow rate of2.0 ml/minute. The re-equilibrated column may bestored with 1×Dilution Buffer with 0.02% sodiumazide at 2–8 °C. Do Not Freeze the column.Table 1.Timetable for IgY 14 LC10 columnDetector Model: 166.ResultsFigure 1.Typical Depletion ChromatogramPeaks A & B aredepleted fractions.Peak C is boundfraction.ABC4AppendixTips for fitting column to most chromatography instruments1.Adapting the column (M6 fitting) to most HPLCsystems• Option 1: female M6 to male 10-32 one-piece adapter (Catalog Number 55069) -Most HPLCinstruments use 10-32 fittings with 1/16″tubing,so an adapter (one-piece fitting) can be used toconnect the column to the instrument. Thisinexpensive approach uses an adapter, a one-piece fitting with female M6 threading at oneend, to accept the male M6 fitting on the tubingconnected to the column, and 10-32 malethreading on the other end, to fit into thedetector or injector.Note: Use of this adapter will require removal ofthe fitting on the injector or detector.• Option 2: female M6/male 10-32 fitting tofemale 10-32/female 10-32 fitting two-pieceadapter (Catalog Number55068) –A two-pieceadapter can be used without changing thedetector or injector fittings on the HPLC. Again,the inexpensive approach is a two-pieceadapter. The female M6 end of the female/malefitting accepts the male M6 fitting from thecolumn, the male 10-32 end fits into one end ofthe female/ female 10-32 fitting. The other endof the female/female fitting accepts the 10-32fitting on the HPLC tubing.2.Adapting the column (M6 fitting) to non-metricmedium/low pressure liquid chromatographysystems• Option 1: female 1/4-28 to male 10-32 one-piece adapter (Catalog Number 55071) plus10-32 to female M6 two-piece adapter (CatalogNumber 55068) -Many medium/low pressuresystems use 1/4-28 fittings. An one-pieceadapter along with a two-piece adapter can beused to connect the column with M6 fittings tothese instruments. This method requires havingtubing with 1/4-28 fittings on the injector anddetector. These fittings will thread into thefemale 1/4-28 to male 10-32 fitting (CatalogNumber 55071). The male 10-32 will tread intothe two-piece adapter (Catalog Number 55068)that will then join to the M6 fitting.• Option 2: female 1/4-28 to female M6 one-piece adapter(Catalog Number 59259-U) -Like Option 1, this option requires the tubing onthe injector and detector already have male1/4-28 fittings. The male 1/4-28 fitting willthread into the adapter that will then join it tothe male M6 fitting.Note:Be sure to order two of these parts, onefor each end of the column.Troubleshooting GuideHigh backpressure -Clogged inlet frits may result in high backpressure, distorted peak shape, and diminished column lifetime. To prevent these problems, remove particulates from samples with a spin filter before loading.No bound fraction peak -Bound proteins can only be removed from the column by eluting with 1×Stripping Buffer. Check LC timetable to ensure enough column exposure time to the 1×Stripping Buffer for complete removal of bound proteins.Abnormal peak height -∼95% of serum/plasma proteins will be removed as the bound fraction. The peak height of the bound fraction is expected to be much greater than that of the flow-through fraction. If this order is reversed, two possibilities may be checked:• Column may not have been regenerated properly after previous use,resulting in lost capacity. Tocorrect this, elute bound proteins with 2 additionalcolumn volumes of 1×Stripping Buffer and thenneutralize and re-equilibrate the column with1×Neutralizing Buffer and 1×Dilution Buffer.• Check for signs of biological growth in the buffer reservoirs. Replace with fresh buffers for optimized column performance.Seppro is a registered trademark of Sigma-Aldrich®Biotechnology LP and Sigma-Aldrich Co.Corning and Spin-X are registered trademarks of Corning, Inc.Delrin and Tefzel are registered trademarks of E.I. du Pont de Nemours & Co., Inc.TD,KR,DEC,MAM,MJM 11/14-2©2014 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at and/or on the reverse side of the invoice or packing slip.。

GT—4310微量快速血糖分析仪的临床应用

GT—4310微量快速血糖分析仪的临床应用

GT—4310微量快速血糖分析仪的临床应用
鄂玉泉;张玉霞
【期刊名称】《中国卫生检验杂志》
【年(卷),期】1993(0)6
【摘要】一种简便、快速、准确的测定血中葡萄糖浓度的方法——积分球式反射光强度法。

检查除用GT—4310血糖仪外,仅用Gluco Pat试带及末梢血50/μl,利用积分球处理的反射光强度,在2min内即求得“BG”浓度。

用本方法测定已知的标准浓度Gluco Pat试带(低、高两个浓度)的值,其SD分别为0.69、0.12;CV%分别为1.55、0.54;测定自配的已知浓度Glucosl标准液(低、
【总页数】1页(P346-346)
【关键词】标准浓度;末梢血;GT;葡萄糖浓度;已知浓度;强度法;反射光;球式;标准液;临床应用
【作者】鄂玉泉;张玉霞
【作者单位】郑州市第二砂轮厂职工医院;郑州棉纺五厂职工医院
【正文语种】中文
【中图分类】R115
【相关文献】
1.微量血糖仪与全自动生化分析仪在糖尿病患者血糖检测中的应用价值 [J], 黄伟忠;廖苑妮;唐景云
2.微量血糖仪与全自动生化分析仪在糖尿病患者血糖检测中的应用价值 [J], 喻彬
3.强生稳步倍加型快速血糖分析仪的临床应用评价 [J], 杨霞
4.东芝TBA-120FR全自动生化分析仪与快速血糖仪检测血糖在糖尿病患者中的临床应用 [J], 邢桂媛;张连凤
5.末梢血快速血糖仪与生化分析仪测定高血压患者血糖的临床比对研究进展 [J], 李嘉
因版权原因,仅展示原文概要,查看原文内容请购买。

亚铁氧化酶(HP)活性检测试剂盒说明书 微量法

亚铁氧化酶(HP)活性检测试剂盒说明书 微量法

亚铁氧化酶(HP )活性检测试剂盒说明书微量法货号:BC4345规格:100T/48S产品组成:使用前请认真核对试剂体积与瓶内体积是否一致,有疑问请及时联系索莱宝工作人员。

试剂名称规格保存条件试剂一液体20 mL×1瓶2-8℃保存试剂二液体3 mL×1瓶2-8℃保存试剂三粉剂×1瓶2-8℃保存标准品液体1 mL×1支2-8℃保存溶液的配制:1、试剂三:临用前加入6 mL 蒸馏水溶解备用,用不完的试剂2-8℃保存4周。

2、标准品:9 µmol/mL 的亚铁离子标准液。

产品说明:亚铁氧化酶(Hsphasetin ,HP )是铜蓝蛋白的同系物,催化亚铁离子(Fe 2+)氧化为三价铁离子(Fe 3+),从而三价铁与转铁蛋白结合,参与细胞铁释放。

以一定浓度的Fe 2+ 为底物,在亚铁氧化酶的催化作用下Fe 2+ 被氧化为Fe 3+,Fe 2+ 与菲咯嗪形成有色复合物,在562 nm 处有特征吸收峰,先计算出未被氧化的Fe 2+ 的含量,进而得出被氧化的Fe 2+ 的含量,可通过Fe 2+被氧化的速率来反映亚铁氧化酶活性。

FeFe 3+Fe 2+[Fe (phen)3]2+ (562nm)注意:实验之前建议选择2-3个预期差异大的样本做预实验。

如果样本吸光值不在测量范围内建议稀释或者增加样本量进行检测。

需自备的仪器和用品:可见分光光度计/酶标仪、微量玻璃比色皿/96孔板、低温离心机、水浴锅/恒温培养箱、可调式移液枪、研钵/匀浆器、冰和蒸馏水、EP 管。

操作步骤:一、样本处理(可适当调整待测样本量,具体比例可以参考文献)1、组织:按照质量(g )︰蒸馏水体积(mL)为1︰5~10的比例(建议称取约0.1 g ,加入1 mL 蒸馏水)加入蒸馏水,冰浴匀浆后于10000 rpm ,4℃离心10 min ,取上清置于冰上待测。

2、血清或血浆:建议用蒸馏水将血清或血浆稀释2~4倍后直接检测。

培养基促生长试验操作规程

培养基促生长试验操作规程

1.0 目的制订培养基灵敏度实验操作,是为了规范、统一培养基灵敏度检测,确保微生物检测准确、安全进行。

2.0 适用范围适用于所有配制好并灭菌的培养基。

3.0 职责3.1. 研究人员负责依据本文件进行培养基促生长试验操作;3.2. 项目负责人负责依据研究项目特性审核本文件的适宜性;3.3. 质量负责人负责监督项目实施过程中的试验符合本文件规定。

4.0 工作流程4.1. 设备、器具压力蒸汽灭菌器、生物安全柜、无菌培养皿、无菌刻度吸管或无菌注射器、无菌玻璃涂布器、酒精灯等。

4.2. 试剂0.9%NaCl、对照培养基、沙氏琼脂、胰酪蛋白大豆消化肉汤培养基4.3. 使用的菌株4.3.1. EP检测用ATCC的菌株:金黄色葡萄球菌Staphylococcus aureus ATCC 6538大肠埃希菌Escherichia coli ATCC 8739铜绿假单胞菌Pseudomonas aeruginosa ATCC 9027枯草芽孢杆菌Bacilllus subtilis ATCC 6633沙门氏菌Salmonella enterica subsp ATCC 14028白色念珠菌Candida albicans ATCC 10231黑曲霉Aspergillus niger ATCC 164044.3.2. 培养基灵敏度检测所用的菌株传代次数不得超过5代, 并采用适宜的菌种保藏技术,以保证试验用菌株的生物学特性。

4.4. 检测频率每批配制、灭菌后的培养基均应做灵敏度测试。

4.5. 操作方法4.5.1. 对照标准培养基用已经过实验证明合格的培养基或购买的有质量证书的成品平皿培养基作为对照标准培养基。

4.5.2. 实验培养基将琼脂类的实验培养基加热融化后,冷却至45℃左右,以无菌的方式在无菌培养皿中注入15~20 mL,备用。

液体类的培养基直接使用。

4.5.3. 菌悬液的制备4.5.3.1. 细菌菌悬液的制备取细菌的斜面培养物1耳匙接种在胰酪蛋白大豆消化肉汤培养基中30~35℃培养18~24h,取上述培养物用0.9%无菌NaCl按10倍系列稀释,分别制成10-7~10-8的菌悬液备用。

  1. 1、下载文档前请自行甄别文档内容的完整性,平台不提供额外的编辑、内容补充、找答案等附加服务。
  2. 2、"仅部分预览"的文档,不可在线预览部分如存在完整性等问题,可反馈申请退款(可完整预览的文档不适用该条件!)。
  3. 3、如文档侵犯您的权益,请联系客服反馈,我们会尽快为您处理(人工客服工作时间:9:00-18:30)。

FEATURE SUMMARYl Most processing power ofPentium-M in a single slot.Reduces $/slot thus addressingmaximum subscribers in asingle slotl Flexibility in configuration anddesign enables reconfigurationand improves re-use ofdevelopment investmentl Powerful storage functionalitybuilt in to enable onboard SASHDD AMCs with fully redundantat a module levell Fibre channel support provideshigh performance storage optionwhen used with a Switch andControl Module with FibreChannel hubl Carrier grade platforms usingrear I/O connectivity Promentum ATCA-4310 ATCA 10-Gigabit Dual-Core Compute Processing Module The ATCA-4310 is a high performance single slot AdvancedTCA compute module. It is ideal for Intel Architecture (IA) based compute intensive applications in IMS (CSCF, Application and Media Servers), IPTV and other network elements such as RNC/BSC and Media Gateways. The ATCA-4310 provides 10-Gigabit fabric connectivity to two dual-core processors, and built in flexibility with dual AMCs sites that can be utilized to support a multitude of application requirements. The NEBS compliant design with rear I/O capability also makes the product a great fit for carrier grade requirements.APPLICATIONS & REQUIREMENTS Next generation communication infrastructure is a packet oriented one in which applications or services such as IMS, IPTV, Radio Network Controllers, VoIP Media Gateways, VPN, speech processing and a multitude of media servers will co-exist in a seamless, unified network. Other applications include SS7 signaling gateways, traffic management/shaping blades, switching and advanced call center applications. The subscriber and traffic growths associated with these services demand significant processing density per slot in order to meet the dollar per slot and the MIPS per slot growth targets placed upon these infrastructure elements. Additionally, designers are seeking flexibility to allow common processing modules to be used across multiple applications in order to gain economies of scale and architectural re-use. Such a requirement demands I/O capability through AMCs with clocking support, front and rear I/O, and bandwidth scalability.PROCESSING DENSITY With dual, dual-core processors and two AMCs for co-processing, I/O and storage, the ATCA-4310 provides exceptional processing density in a thermally efficient design, which equates to great performance per watt in a single slot solution. The ATCA-4310 is fully compliant with PICMG 3.0 and PICMG 3.1 for use with Ethernet base interface and fabric interface systems. The module utilizes two Dual-Core Intel Xeon LV/ULV processors along with the Intel E7520 chipset, and provides support for up to 16GB of DDR2-400 ECC SDRAM. Configuration flexibility is supported with two mid-size AMC sites, or one double-wide mid-size AMC site, which allows a variety of AMC module options, such as co-processors, disk, LAN or WAN adapters. The module also optionally supports a local and remote SAS interfaceenabling SAS disk support via an AMC disk module.CONFIGURATION FLEXIBILITYThe ATCA-4310 and its configuration capability make it a versatile option for a multitude of applications. Theconfiguration capabilities include:l Number of processors: one or two dual-core processorsl Memory configurations for DRAM: Up to 16 GB via 4 DIMM socketsl PICMG 3.1 Fabric configurations supporting options 1 and 9l AMC configurations including fabric connectivity and storagel Network Timing Subsystem for clocking support for WAN I/O AMCsl Storage configurations: Secure Digital (SDHC) socket for optional flash card, redundant on-board 256MB flashmodules, SAS on board and off board with rear I/O connectivityThe functionality of the ATCA-4310 can be configured through the use of two AMC sites, eliminating the need foradditional slots. Optional AMC modules include SAS and SATA storage, Co-Processors, LAN and WAN I/O adapters.For applications requiring local storage, the ATCA-4310 can accommodate an optional SAS or SATA based AMC or optional secure digital (SD) flash card. The SAS connectivity is designed to make use of Rear Transition Module to provide redundancy through cross cabling to another compute module in an adjacent slot. Such cross cabling enables redundancy in storage through dual-hosted disk drives.REAR TRANSITION MODULEThe ATCA-4310 is designed for carrier grade applications demanding rear I/O connectivity. It includes an independently hot swappable RTM for rear I/O connectivity. The RTM includes standard LED support and connectors for RS-232, SAS and alarm I/O.SYSTEM MANAGEMENT, RELIABILITY AND HIGH AVAILABILITY The ATCA-4310 is designed for High Availability (HA) applications providing 99.999% up time. An Intelligent PlatformPromentum ATCA-4310 SpecificationsPromentum ATCA-4310 DATA SHEET | © 2008 RadiSys Corporation | ID: 10-30-2008 7 | Page 1 of 4Management Controller (IPMC) provides system management functionality compliant with the IPMI specification, andincludes features such as standard e-keying, remote upgrade capability, IPMI-over-LAN, Serial-over-LAN, and message bridging and messaging support.SOFTWARE SUPPORTThe ATCA-4310 supports RedHat Linux, Wind River PNE LE 1.x, and MontaVista Carrier Grade Linux 4.x. Support forother Linux versions is available through RadiSys service options.FEATURE FUNCTION DESCRIPTIONPHYSICAL Dimensions8Ux6HPx280mm ATCA, single slot Compliance PICMG 3.0, PICMG 3.1 (option 1 and 9), AMC.0, AMC.1, AMC.2, AMC.3PROCESSOR SYSTEM CPU x2 2.0GhzDual-Core Intel Xeon LV Processors (Sossaman)Cache Integrated 2MB L2Chipset Intel E7520 with 6300ESB FSB 667MHzMEMORY Technology Registered DDR2-400, with ECC Capacity Up to 16GB Sockets Four 240-pin DIMMETHERNET BASE Interface Dual 10/100/1000BaseBX ControllerIntel 82571EB ETHERNET FABRIC Fabric (PICMG 3.1, option 1, 9)10GBase-BX4, 1000Base-BX Controller Intel 82598EBAMC SITES Site 1Single Mid-size, AMC.0 compliant. AMC.1 Type 4 x4 PCI Express to E7520, AMC.3 SAS or SATA to SAS controller Site 2Single Mid-size, AMC.0 compliant. AMC.1 Type 4 x4 PCI Express to E7520, AMC.2 Type E2 dual GbE to CPU, AMC.3 SATA to 6300ESB SATA controller HARD DRIVE INTERFACE InterfaceSAS and SATA ControllerIntegrated ConnectorPer AMC.3FRONT PANEL I/O USBOne Type A connector SerialRJ-45, RS-232EthernetRJ-45, 10/100/1000BaseT ResetRecessed push button RTM I/O (OPTIONAL) ConnectorsRS-232 (RJ-45), SAS and Alarm I/O Hot SwapSupported FRU IDIndependent FRU ID LEDsHot Swap (Blu), Power Good, and Fault CONNECTORS,BACKPLANE Power, IPMB ATCA Zone 1, P10 connector Ethernet Base Fabric ATCA Zone 2, J23 connectorPromentum ATCA-4310 SpecificationsFEATURE FUNCTION DESCRIPTIONPHYSICAL Dimensions8Ux6HPx280mm ATCA, single slotCompliance PICMG 3.0, PICMG 3.1 (option 1 and 9), AMC.0, AMC.1, AMC.2, AMC.3PROCESSOR SYSTEM CPU x2 2.0GhzDual-Core Intel Xeon LV Processors (Sossaman)Cache Integrated 2MB L2Chipset Intel E7520 with 6300ESBFSB667MHzMEMORY Technology Registered DDR2-400, with ECCCapacity Up to 16GBSockets Four 240-pin DIMMETHERNET BASE Interface Dual 10/100/1000BaseBXController Intel 82571EBETHERNET FABRIC Fabric (PICMG 3.1, option 1, 9)10GBase-BX4, 1000Base-BXController Intel 82598EBAMC SITES Site 1Single Mid-size, AMC.0 compliant. AMC.1 Type 4 x4 PCI Express to E7520, AMC.3 SAS or SATA to SAS controllerSite 2Single Mid-size, AMC.0 compliant. AMC.1 Type 4 x4 PCI Express to E7520, AMC.2 Type E2 dual GbE to CPU, AMC.3 SATA to6300ESB SATA controllerHARD DRIVE INTERFACE Interface SAS and SATAController IntegratedConnector Per AMC.3FRONT PANEL I/O USB One Type A connectorSerial RJ-45, RS-232Ethernet RJ-45, 10/100/1000BaseTReset Recessed push buttonRTM I/O (OPTIONAL)Connectors RS-232 (RJ-45), SAS and Alarm I/OHot Swap SupportedFRU ID Independent FRU IDLEDs Hot Swap (Blu), Power Good, and FaultCONNECTORS,BACKPLANEPower, IPMB ATCA Zone 1, P10 connectorEthernet Base Fabric ATCA Zone 2, J23 connectorEthernet Fabric ATCA Zone 2 J23 connectorSynchronization Clock ATCA Zone 2, J20 connectorUpdate Channel ATCA Zone 2, J20 connectorRTM ATCA Zone 3, J30 connectorLEDS-Hot Swap (Blu), OOS (Red/Yel), Power Good (Grn), Blade Status (Tri)POWER-200 W (maximum)ENVIRONMENT Ambient Temperature+5° to +45°C (operating)-40° to +70°C (non-operating)Relative Humidity (non-condensing)5% to 85% (operating) 5% to 90% (non-operating)REGULATORY Safety UL/EN/IEC 60950-1, CSA 22.2EMC FCC Part 15, Class B, EN 55022: 2006, Class BPromentum ATCA-4310 DATA SHEET | © 2009 RadiSys Corporation | ID: 10-30-2008 7 | Page 2 of 4 * All other trademarks are the properties of their respective owners.Promentum ATCA-4310 DATA SHEET | ©2009 RadiSys Corporation | ID: 10-30-2008 7 | Page 4 of 4EN 61000-4-2, EN 61000-4-3, EN 61000-4-4, EN 61000-4-5, EN 61000-4-6, EN 61000-4-8 WARRANTY-Two years, parts onlyOrdering InformationCall for order code configuration, pricing and availability.2009 RadiSys Corporation. RadiSys is a registered trademark of RadiSys Corporation. Convedia, Microware and OS-9are registered trademarks of RadiSys Corporation. Promentum, and Procelerant are trademarks of RadiSysCorporation. *All other trademarks are the properties of their respective owners. All specifications within this documentare subject to change without notice.Promentum ATCA-4310 DATA SHEET | ©2009 RadiSys Corporation | ID: 10-30-2008 7 | Page 4 of 4Ordering InformationCall for order code configuration, pricing and availability.2009 RadiSys Corporation. RadiSys is a registered trademark of RadiSys Corporation. Convedia, Microware and OS-9 are registered trademarks of RadiSys Corporation. Promentum, and Procelerant are trademarks of RadiSysCorporation. *All other trademarks are the properties of their respective owners. All specifications within this document are subject to change without notice.。

相关文档
最新文档