免疫胶体金法实验流程

免疫胶体金法实验流程
(中英文实用版)
英文文档内容：
Immunogold Colloidal Gold Assay Experimental Procedure
The immunogold colloidal gold assay is a widely used technique in immunochemistry, introduced by Faulk and Taytor in 1971.This method has gained increasing application in various fields of biomedical research.The main applications in medical testing include immunochromatography and rapid immunogold filtration assay (DIGFA), which are used for the detection of HBsAg, HCG, and anti-double-stranded DNA antibodies, among others.This assay is characterized by its simplicity, rapidity, accuracy, and lack of pollution.
The immunogold colloidal gold assay is based on the synthesis of gold nanoparticles from chloroauric acid (HAuCl4) in the presence of reducing agents such as white phosphorus, ascorbic acid, sodium citrate (柠檬酸钠), and tannic acid.These gold nanoparticles are formed with a certain size and stabilized in a colloidal state due to electrostatic interactions, resulting in a negatively charged hydrophobic colloidal solution.The colloidal gold is considered an ideal immunological marker for immunoelectron microscopy.
To perform the immunogold colloidal gold assay, the following experimental procedure is typically followed:
1.Prepare the gold nanoparticles by reducing chloroauric acid with a suitable reducing agent.
bel the target antigens or antibodies with the gold nanoparticles.
3.Coat the gold-labeled antigens or antibodies onto a solid support, such as a nitrocellulose membrane or a microplate.
4.Prepare the sample containing the target analyte to be tested.
5.Perform the immunochromatography or rapid immunogold filtration assay according to the manufacturer"s instructions.
6.Interpret the results by visualizing the presence or absence of the target analyte on the solid support.
This assay provides a rapid and sensitive method for the detection and characterization of various biological targets, making it an essential tool in diagnostic medicine and research.
中文文档内容：
免疫胶体金法实验流程
免疫胶体金法是一种在免疫化学中广泛应用的技术，由Faulk和Taytor在1971年引入。

该方法在生物医学研究的各个领域中日益得到应用。

在医学检验中，主要应用于免疫层析法和快速免疫金渗滤法（DIGFA），用于检测HBsAg、HCG和抗双链DNA抗体等，具有简单、快速、准确和无污染等优点。

该实验方法基于在氯金酸（HAuCl4）存在的条件下，利用还原剂如白磷、抗坏血酸、柠檬酸钠（柠檬酸钠）和鞣酸合成金纳米颗粒。

这些金纳米颗粒具有一定的大小，并通过静电作用稳定在胶体状态下，形成带负电的疏水胶溶液。

免疫胶体金被认
为是免疫电镜技术中理想的免疫标记物。

要进行免疫胶体金法实验，通常遵循以下实验流程：
1.通过合适的还原剂还原氯金酸以制备金纳米颗粒。

2.用金纳米颗粒标记目标抗原或抗体。

3.将金标记的抗原或抗体涂覆在固体支持物上，如硝化纤维素膜或微板。

4.准备包含目标分析物的样品进行测试。

5.根据制造商的说明执行免疫层析法或快速免疫金渗滤法。

6.通过视觉化固体支持物上目标分析物的存在或不存在来解释结果。