imagej的使用分析荧光图片
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ImageJ Features
Runs Everywhere:
ImageJ is written in Java, which allows it to run on Linux, Mac OS X and Windows, in both 32-bit and 64-bit modes.
Open Source:
If you know the size of a feature (previously applied scale bar for instance) you can use this command to apply a calibration.
Using the line selection tool, draw a ling along the length of the feature/scale bar.
via plugins installed in the Import submenu.
Subtract background
Merge channel
Scale bar
Scale bars should be present on all publication/presentation images/movies. It’s worth putting them in sooner rather than later. Choose a standard scale bar size for all your images if possible to avoid confusion.
ImageJ and its Java source code are freely available and in the public domain. No license is required.
Plugins:
Extend ImageJ by developing plugins using ImageJ's built in text editor and Java compiler. More than 500 plugins are available.
this process. Use Image>Adjust>Threshold
to threshold an image.
Selection and crop
Thank you !
2011-4-19
Run the menu command “Plugins/Spatial calibration/Set scale”[1].
Enter the dimensions of the object/scale bar in
the “known distance” box and set the units in the Unit of length box.
Open
Reads an image and displays it in a separate window. Files must be in TIFF, GIF, JPEG,
DICOM, BMP, PGM or FITS format. Also opens ImageJ and NIH Image lookup tables (with ".lut" extension). Additional file formats are supported
Analysis:
Measure area, mean, standard deviation, min and max of selection or entire image. Measure lengths and angles. Use real world measurement units such as millimeters. Calibrate using density standards. Generate histograms and profile plots.
Do not check Global unless you wish all your
images to have this calibration! Click OK.
Hale Waihona Puke Baidu
Analyze Particles and count cell
This command counts and measures objects in binary or thresholded images. It works by scanning the image or selection until it finds the edge of an object. It then outlines the object using the wand tool, measures it using the Measure command, fills it to make it invisible, then resumes scanning until it reaches the end of the image or selection. Press the esc key to abort
Installation
Linux Mac OS X Mac OS 9 Windows
Windows http://imagej.nih.gov/ij/download.html
Download ImageJ 1.44 bundled with 32-bit Java 1.6.0_20 (28MB), with 64bit Java 1.6.0_20 (24MB; requires 64bit Windows) or without Java (3MB).
Runs Everywhere:
ImageJ is written in Java, which allows it to run on Linux, Mac OS X and Windows, in both 32-bit and 64-bit modes.
Open Source:
If you know the size of a feature (previously applied scale bar for instance) you can use this command to apply a calibration.
Using the line selection tool, draw a ling along the length of the feature/scale bar.
via plugins installed in the Import submenu.
Subtract background
Merge channel
Scale bar
Scale bars should be present on all publication/presentation images/movies. It’s worth putting them in sooner rather than later. Choose a standard scale bar size for all your images if possible to avoid confusion.
ImageJ and its Java source code are freely available and in the public domain. No license is required.
Plugins:
Extend ImageJ by developing plugins using ImageJ's built in text editor and Java compiler. More than 500 plugins are available.
this process. Use Image>Adjust>Threshold
to threshold an image.
Selection and crop
Thank you !
2011-4-19
Run the menu command “Plugins/Spatial calibration/Set scale”[1].
Enter the dimensions of the object/scale bar in
the “known distance” box and set the units in the Unit of length box.
Open
Reads an image and displays it in a separate window. Files must be in TIFF, GIF, JPEG,
DICOM, BMP, PGM or FITS format. Also opens ImageJ and NIH Image lookup tables (with ".lut" extension). Additional file formats are supported
Analysis:
Measure area, mean, standard deviation, min and max of selection or entire image. Measure lengths and angles. Use real world measurement units such as millimeters. Calibrate using density standards. Generate histograms and profile plots.
Do not check Global unless you wish all your
images to have this calibration! Click OK.
Hale Waihona Puke Baidu
Analyze Particles and count cell
This command counts and measures objects in binary or thresholded images. It works by scanning the image or selection until it finds the edge of an object. It then outlines the object using the wand tool, measures it using the Measure command, fills it to make it invisible, then resumes scanning until it reaches the end of the image or selection. Press the esc key to abort
Installation
Linux Mac OS X Mac OS 9 Windows
Windows http://imagej.nih.gov/ij/download.html
Download ImageJ 1.44 bundled with 32-bit Java 1.6.0_20 (28MB), with 64bit Java 1.6.0_20 (24MB; requires 64bit Windows) or without Java (3MB).