引物设计1

相关主题

1、下载文档前请自行甄别文档内容的完整性，平台不提供额外的编辑、内容补充、找答案等附加服务。
2、"仅部分预览"的文档,不可在线预览部分如存在完整性等问题,可反馈申请退款(可完整预览的文档不适用该条件!)。
3、如文档侵犯您的权益，请联系客服反馈,我们会尽快为您处理(人工客服工作时间：9:00-18:30)。

1-2890(引物1)

#1: Product of length 640 (rating: 171)

Contains region of the molecule from 1 to 640

Tm: 72.1 C TaOpt: 48.8 C GC: 32.3

Sense Primer:

CCTGGTTAATCCAAATCAC

Similarity: 100.0%

Length: 19 Tm: 44.1 C GC: 42.1

dH: -142.0 kcal/mol dS: -372.4 cal/mol dG: -29.2 kcal/mol

Antisense Primer:

GACAGGCCCTAATTAAGTT

Similarity: 100.0%

Length: 20 Tm: 45.0 C GC: 42.1

dH: -158.0 kcal/mol dS: -418.4 cal/mol dG: -31.5 kcal/mol

Tm Difference: 0.9

GC Difference: 0

#1: Product of length 540 (rating: 171)

Contains region of the molecule from 1 to 540

Tm: 72.2 C TaOpt: 49.4 C GC: 33.1

Sense Primer:

CCTGGTTAATCCAAATCACT

Similarity: 100.0%

Length: 20 Tm: 45.8 C GC: 40.0

dH: -149.8 kcal/mol dS: -393.2 cal/mol dG: -30.8 kcal/mol

Antisense Primer:

ATAAGATTTGAGGTCAGCCA

Similarity: 100.0%

Length: 20 Tm: 46.4 C GC: 40.0

dH: -147.7 kcal/mol dS: -386.3 cal/mol dG: -30.7 kcal/mol

Tm Difference: 0.6

GC Difference: 0.0

1-2890(引物2)

#1: Product of length 603 (rating: 171)

Contains region of the molecule from 514 to 1116

Tm: 73.9 C TaOpt: 50.3 C GC: 37.0

Sense Primer:

TTGAAGATGGCTGACCT

Similarity: 100.0%

Length: 18 Tm: 42 C GC: 47.1

dH: -129.6 kcal/mol dS: -335.0 cal/mol dG: -27.9 kcal/mol

Antisense Primer:

GGAGGCCCTTTAACTTAA

Similarity: 100.0%

Length: 20 Tm: 43.7 C GC: 44.4

dH: -165.8 kcal/mol dS: -438.8 cal/mol dG: -33.2 kcal/mol

Tm Difference: 2.0

GC Difference: 10.0

1-2890(引物3)

#1: Product of length 703 (rating: 171)

Contains region of the molecule from 1063 to 1765

Tm: 72.7 C TaOpt: 51.0 C GC: 33.6

Sense Primer:

GATGCAAATTCAAATCCAGTGG

Similarity: 100.0%

Length: 22 Tm: 53.6 C GC: 40.9

dH: -164.5 kcal/mol dS: -425.5 cal/mol dG: -35.9 kcal/mol

Antisense Primer:

GGTATGCATGGAGGTGATTTAT

Similarity: 100.0%

Length: 22 Tm: 50.0 C GC: 40.9

dH: -163.9 kcal/mol dS: -428.7 cal/mol dG: -34.3 kcal/mol

Tm Difference: 3.6

GC Difference: 0.0

1-2890(引物4)

#1: Product of length 550 (rating: 171)

Contains region of the molecule from 1644 to 2193

Tm: 73.3 C TaOpt: 49.4 C GC: 35.6

Sense Primer:

GAAGGCAATCAATCTACTTG

Similarity: 100.0%

Length: 20 Tm: 44.0 C GC: 40.0

dH: -145.8 kcal/mol dS: -383.7 cal/mol dG: -29.6 kcal/mol

Antisense Primer:

CTTAAGCCACTTTCTGCA

Similarity: 100.0%

Length: 18 Tm: 43.4 C GC: 44.4

dH: -136.3 kcal/mol dS: -356.4 cal/mol dG: -28.3 kcal/mol

Tm Difference: 0.6

GC Difference: 4.4

1-2890(引物5)