酶切位点汇总
常见分子实验限制性内切酶酶切位点大全
Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI识别位点BanI识别位点BanII识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点与大家共享（非原创）。
酶切位点汇总
酶切位点汇总
酶切位点，又称为限制性内切酶位点，是指DNA分子上特定的序列，这些序列是限制
性内切酶可以识别和切割的地方。
限制性内切酶是一种在细菌和其它生物中广泛存在的酶，能够切割或切除一个或多个DNA碱基对。
这些限制性内切酶在生物技术领域广泛应用，用
于DNA序列分析、DNA重组、基因工程等方面。
以下是常见的几种酶切位点：
1. EcoRI切割位点是5′-GAATTC-3′，这是一种广泛应用的限制性内切酶，通常用于DNA纯化、制备DNA载体等。
2. BamHI切割位点是5′-GGATCC-3′，BamHI能够切割链间，产生具有黏性末端的DNA 序列。
常被用于制备双链DNA的黏性末端。
4. PstI切割位点是5′-CTGCAG-3′，PstI是一种双切酶，可以切割成不同长度的DNA 序列，适用于构建多种不同长度的DNA分子。
总之，酶切位点及其对应的限制性内切酶在现代生物领域有着广泛的应用和重要的作用。
了解不同的酶切位点是有很大帮助的，它可以为实验设计和分子生物学研究提供基础。
同时，也让我们更好地理解限制性内切酶在DNA分子上的作用，帮助我们在生物技术领域
更加熟练地掌握其应用。
酶切位点——精选推荐
PCR引物设计时酶切位点的保护碱基表
不同内切酶对识别位点以外最少保护碱基数目的要求（在本表中没有列出的酶，则通常需在识别位点两端至少加上6个保护碱基，以确保酶切反应的进行。
）
注释
1.如果要加在序列的5’端，就在酶切位点识别碱基序列（红色）的5’端加上相
应的碱基（黑色），如果要在序列的3’端加上保护碱基，就在酶切位点识别碱基序列（红色）的3’端加上相应的碱基（黑色）。
2.切割率：正确识别并切割的效率。
3.加保护碱基时最好选用切割率高时加的相应碱基。
常用限制性内切酶酶切位点汇总
Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI 识别位点BanI识别位点BanII识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I 识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点Welcome!!!欢迎您的下载，资料仅供参考！。
常用限制性内切酶酶切位点总结
常用限制性内切酶酶切位点总结————————————————————————————————作者：————————————————————————————————日期：Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI识别位点BanI识别位点BanII识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。
常用限制性内切酶酶切位点汇总复习过程
常用限制性内切酶酶切位点汇总Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI识别位点BanI识别位点BanII识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点 KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI 识别位点MnlI识别位点MscI 识别位点MseI 识别位点MslI识别位点MspA1I识别位点MspI 识别位点MwoI识别位点NaeI识别位点NarI 识别位点NciI 识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII 识别位点NlaIV识别位点NmeAIII识别位点NotI 识别位点NruI识别位点NsiI识别位点NspI 识别位点PacI 识别位点PaeR7I 识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI 识别位点PmlI识别位点PpuMI 识别位点PshAI 识别位点PsiI识别位点PspGI识别位点PspOMI 识别位点PspXI识别位点PstI识别位点PvuI 识别位点PvuII 识别位点RsrII识别位点SacI 识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI 识别位点ScaI 识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI 识别位点SmlI识别位点SnaBI识别位点SpeI 识别位点SphI识别位点SspI识别位点StuI 识别位点StyD4I 识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI 识别位点TseI识别位点Tsp45I 识别位点Tsp509I 识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI 识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。
常用酶切位点表(含保护碱基)课件.doc
切割率%酶寡核苷酸序列2 hr 20 hrAcc I G GTCGAC C 0 0CG GTCGAC CG 0 0CCG GTCGAC CGG 0 0Afl III C ACATGT G 0 0CC ACATGT GG >90 >90CCC ACATGT GGG >90 >90Asc I GGCGCGCC >90 >90A GGCGCGCC T >90 >90TT GGCGCGCC AA >90 >90Ava I C CCCGGG G 50 >90CC CCCGGG GG >90 >90TCC CCCGGG GGA >90 >90BamH I C GGATCC G 10 25CG GGATCC CG >90 >90CGC GGATCC GCG >90 >90Bgl II C AGATCT G 0 0GA AGATCT TC 75 >90GGA AGATCT TCC 25 >90BssH II G GCGCGC C 0 0AG GCGCGC CT 0 0TTG GCGCGC CAA 50 >90BstE II G GGT(A/T)ACC C 0 10BstX I AACTGCAGAA CCAATGCATTGG 0 0 AAAACTGCAG CCAATGCATTGG AA 25 50CTGCAGAA CCAATGCATTGG ATGCAT 25 >90Cla I C ATCGAT G 0 0GATCGAT C 0 0CC ATCGAT GG >90 >90CCC ATCGAT GGG 50 50EcoR I G GAATTC C >90 >90>90 >90CG GAATTC CGCCG GAATTC CGG >90 >90Hae III GG GGCC CC >90 >90AGC GGCC GCT >90 >90TTGC GGCC GCAA >90 >90Hind III C AAGCTT G 0 0CC AAGCTT GG 0 0CCC AAGCTT GGG 10 75Kpn I G GGTACC C 0 0GG GGTACC CC >90 >90CGG GGTACC CCG >90 >90Mlu I G ACGCGT C 0 0CG ACGCGT CG 25 50Nco I C CCATGG G 0 0CATG CCATGG CATG 50 75Nde I C CATATG G 0 0CC CATATG GG 0 0CGC CATATG GCG 0 0GGGTTT CATATG AAACCC 0 0GGAATTC CATATG GAATTCC 75 >90GGGAATTC CATATG GAATTCCC 75 >90Nhe I G GCTAGC C 0 0CG GCTAGC CG 10 25CTA GCTAGC TAG 10 50切割率%酶寡核苷酸序列2 hr 20 hrNot I TT GCGGCCGC AA 0 0ATTT GCGGCCGC TTTA 10 10AAATAT GCGGCCGC TATAAA 10 10 ATAAGAAT GCGGCCGC TAAACTAT 25 90 AAGGAAAAAA GCGGCCGC AAAAGGAAAA 25 >90Nsi I TGC ATGCAT GCA 10 >90 CCA ATGCAT TGGTTCTGCAGTT >90 >90Pac I TTAATTAA 0 0G TTAATTAA C 0 25CC TTAATTAA GG 0 >90Pme I GTTTAAAC 0 0G GTTTAAAC C 0 25GG GTTTAAAC CC 0 50AGCTTT GTTTAAAC GGCGCGCCGG 75 >90Pst I G CTGCAG C 0 0TGCA CTGCAG TGCA 10 10 AA CTGCAG AACCAATGCATTGG >90 >90AAAA CTGCAG CCAATGCATTGGAA >90 >90CTGCAG AACCAATGCATTGGATGCAT 0 0Pvu I C CGATCG G 0 0AT CGATCG AT 10 25TCG CGATCG CGA 0 10 Sac I C GAGCTC G 10 10Sac II GCCGCGG C 0 0TCC CCGCGG GGA 50 >90Sal I GTCGAC GTCAAAAGGCCATAGCGGCCGC 0 0 GC GTCGAC GTCTTGGCCATAGCGGCCGCG 10 50G 10 75ACGC GTCGAC GTCGGCCATAGCGGCCGCGGAASca I GAGTACT C 10 25AAA AGTACT TTT 75 75Sma I CCCGGG 0 10CCCCGGG G 0 10CC CCCGGG GG 10 50TCC CCCGGG GGA >90 >90Spe I GACTAGT C 10 >90GG ACTAGT CC 10 >90CGG ACTAGT CCG 0 50CTAG ACTAGT CTAG 0 50Sph I G GCATGC C 0 0CAT GCATGC ATG 0 25ACAT GCATGC ATGT 10 50Stu I AAGGCCT T >90 >90GA AGGCCT TC >90 >90AAA AGGCCT TTT >90 >90Xba I CTCTAGA G 0 0GC TCTAGA GC >90 >90TGC TCTAGA GCA 75 >90CTAG TCTAGA CTAG 75 >90Xho I C CTCGAG G 0 0CC CTCGAG GG 10 25CCG CTCGAG CGG 10 75Xma I CCCCGGG G 0 0CC CCCGGG GG 25 75CCC CCCGGG GGG 50 >90TCCC CCCGGG GGGA >90 >90。
限制性内切酶酶切位点汇总
ApaI识别位点Acc65I识别位点ApaLI识别位点AccI识别位点ApeKI识别位点AciI识别位点ApoI识别位点AclI识别位点AscI识别位点AcuI识别位点AseI识别位点AfeI识别位点AsiSI识别位点AflII识别位点AvaI识别位点AflIII识别位点AvaII识别位点AgeI识别位点AvrII识别位点AhdI识别位点BaeI识别位点AleI识别位点BamHI识别位点AluI识别位点BanI识别位点AlwI识别位点BanII识别位点AlwNI识别位点BmrI识别位点BbvCI识别位点BmtI识别位点BbvI识别位点BpmI识别位点BccI识别位点Bpu10I识别位点BceAI识别位点BpuEI识别位点BcgI识别位点BsaAI识别位点BciVI识别位点BsaBI识别位点BclI识别位点BsaHI识别位点BfaI识别位点BsaI识别位点BfuAI识别位点BsaJI识别位点BglI识别位点BsaWI识别位点BglII识别位点BsaXI识别位点BlpI识别位点BseRI识别位点Bme1580I识别位点BseYI识别位点BmgBI识别位点BspMI识别位点BsiEI识别位点BspQI识别位点BsiHKAI识别位点BsrBI识别位点BsiWI识别位点BsrDI识别位点BslI识别位点BsrFI识别位点BsmAI识别位点BsrGI识别位点BsmBI识别位点BsrI识别位点BsmFI识别位点BssHII识别位点BsmI识别位点BssKI识别位点BsoBI识别位点BssSI识别位点Bsp1286I识别位点BstAPI识别位点BspCNI识别位点BstBI识别位点BspDI识别位点BstEII识别位点BspEI识别位点BstNI识别位点BspHI识别位点DdeI识别位点BstXI识别位点DpnI识别位点BstYI识别位点BstZ17I识别位点DpnII识别位点Bsu36I识别位点DraI识别位点BtgI识别位点DraIII识别位点BtgZI识别位点DrdI识别位点BtsCI识别位点EaeI识别位点BtsI识别位点EaeI识别位点Cac8I识别位点EagI识别位点ClaI识别位点EarI识别位点CspCI识别位点EciI识别位点CviAII识别位点EcoNI识别位点CviKI-1识别位点EcoO109I识别位点CviQI识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。
史上最全限制性内切酶酶切位点汇总
HgaI识别位点 HhaI识别位点 HincII识别位点 HindIII识别位点 HinfI识别位点 HinP1I识别位点 HpaI识别位点 HpaII识别位点 HphI识别位点 Hpy188I识别位点 Hpy188III识别位点 Hpy99I识别位点 HpyAV识别位点 HpyCH4III识别位点 HpyCH4IV
HpyCH4V识别位点 KasI识别位点 KpnI识别位点 MboI识别位点 MboII识别位点 MfeI识别位点 MluI识别位点 MlyI识别位点 MmeI识别位点 MnlI识别位点 MscI识别位点 MseI识别位点 MslI识别位点 MspA1I识别位点
MspI识别位点 MwoI识别位点 NaeI识别位点 NarI识别位点 NciI识别位点 NcoI识别位点 NdeI识别位点 NgoMIV识别位点 NheI识别位点 NlaIII识别位点 NlaIV识别位点 NmeAIII识别位点
SbfI识别位点 ScaI识别位点 ScrFI识别位点 SexAI识别位点 SfaNI识别位点 SfcI识别位点 SfiI识别位点 SfoI识别位点 SgrAI识别位点 SmaI识别位点 SmlI识别位点 SnaBI识别位点 SpeI识别位点 SphI识别位点
SspI识别位点 StuI识别位点 StyD4I识别位点 StyI识别位点 SwaI识别位点 TaqαI识别位点 TfiI识别位点 TliI识别位点 TseI识别位点 Tsp45I识别位点 Tsp509I识别位点 TspMI识别位点
NotI识别位点 NruI识别位点
NsiI识别位点 NspI识别位点 PacI识别位点 PaeR7I识别位点 PciI识别位点 PflFI识别位点 PflMI识别位点 PhoI识别位点 PleI识别位点 PmeI识别位点 PmlI识别位点 PpuMI识别位点 PshAI识别位点 PsiI识别位点
史上最全限制性内切酶酶切位点汇总
专业资料分享A系列Aatll识别位点…G ACGT匕…一丁3：.. CJGC AG,, .5^Acc65l识别位点5…GFMK AC …3，3一. CA KMJG. . 5JAccl识别位点5…Gl\lK AC …丁3J. . CA KMJG. . 5JAcil识别位点5 …dtGC …3’ 宙…GG空…莎Acll识别位点5 …A A\；GTT .33\ .. T TG* A (X)Acul识别位点5；. CTG A AG(N). \ . . 3J 3d.. ,GACTTC(N)U A... 5J Afel识别位点5\.. AGC^CT..,3,3 …TCGJCGA..,5JAflll 识别位点5 …(fTT A AG…3，3：…G A A T T& …5"Afllll 识别位点5；.. ^CRYGT , ,3 3：…TGYRqA. . ,5Agel识别位点5P. . /fCCGGT , . .3J3；. . TGGCC^A. , .5^ Ahdl识别位点5P. G ACNN F/N NGTC... 3P 3- CTGNI^NNNCAG^.S^ Alel识别位点5；. .CACNt/NNGTG 3' 3. ..GTGNI^NNCAC, .. h Alul识别位点5 . .. A(jfcT….3P 3…TQSA-SAlwl识别位点5 r,GGATC(N)7「3"3. .. CCTAGIN)^..^AlwNl识别位点5：. CAGNNN T CTG..3F 3；. GTC^NNNG AC.. .5*Apal识别位点5 .., GGGCtft. ..3P3\., C^CCGGG .ApaLl识别位点5\.. (^TGCAC.. ,3J3 .. CACGTjGApeKl识别位点5P. . . t^CWGC., .3r3\. CGWCjp., .5^Apol识别位点5；,. FLATTY,, ,3J3 , _ Y TTA AJl. T.5"Ascl识别位点5：., GG^bGCGCC, .33 CCGCGCJ J G, .5JAsel识别位点5 . B 4A T't A A T t. , 3J3 . .. T A A TJ A .. 5JAsiSl识别位点5...GCGAl1SGC r. 3r3...CGCJAGCG . .5Aval识别位点可…C TV CGRG…3」3：..GRGCYJ>..hAvall识别位点5：…dfewCC,.,3r3；., CCWG^.. 5JAvrll识别位点5... C T CT AGG .3’…GGATCf…5」Bael识别位点5；^(NJACIN^GTAYCCNjJ 33.^^NJTGjN^CATRGtN)^ .5^识别位点吕,"・G E AT CC…3’BamHl专业资料分享3’…C C T A Gp -.. 5"Banl识别位点吕…G^YRCC^ 33 …CCRYQp^ .5JBanII识别位点5：.. GRGC Y^t …3",C A Y C G R G . ,,Bbsl识别位点5. .,GAAGAC (N)/ . .3r3J. .. 0 T T C T G 减丄…S' BbvCI识别位点5 …C(T T CAGC.”.3*3 …GGAGT A CG.,S JBbvl识别位点5 …GCAGCHN}；…3* 計…CQTCG(N)iar .5^Bccl识别位点5P. . CCATC(N)/,. 33\ , .GGTAG(N). -5rkBceAl识别位点5... ACGGCtNjJ. ,3^3：.. TGCCG(N)F「5*Bcgl识别位点5 . T4N)CGA(NK TGC(N)J . .3J 3；.^(N)GCT(N)；ACG{N)^.b5^BciVl识别位点5. r,GT ATCC (N)J .3F3. ..C AT AGG (N}t^ ..5PBcll识别位点5... T'feATC A,- 3J3：…ACT AGJ., 5JBfal识别位点5：.. (?T AG . .3r苗…GA.C…hBfuAl识别位点5；. . ACCTGC(N)J 3’3. ..TGG ACG(N)^, .5^Bgll识别位点5；. GCCNNNN^JGGC., 3P3 . . CGGr^hlNNNCCG. , 5*Bglll识别位点莎…A T G T…3’3：”. TCTAt^A.. 5rBlpl识别位点5•…G(7TN AGO., -3J3；., CGAN T；CG , .5^Bme1580l识别位点5 . . GKGCWit …才3 . . Q^MCGKG ,5JBmgBl识别位点h…C AC令TC…孑3 . . .GTGJ： AG. . 5*Bmrl识别位点5... ACTGGG(N) T r.3r3... TGACCC(N)4r..5-Bmtl识别位点h…GCTA(?b …3’3\, C/3 ATCG …5」Bpml识别位点5 .… CTGGAG(N)1P T 3r3... GACCTC(N)14j...5rBpulOI识别位点5’…CCT T N AGC …*33\., GGAN^CG .. 5BpuEl识别位点5；.. CT TG AG(N)r T,.3'3\.. GAACTC(N)…A...S'BsaAl识别位点5；.. YAC'GTR . .3^BsaBl识别位点5： ..GATNN^NATC , .3^3：, ,CTANN/1NTAG,. . 5^BsaHl识别位点5P. . .GFitG YC . . ,3^3...C YGC^RG.. .5JBsal识别位点5... GGTCTC (N)/. .3^3... C C A G A G (N)^. . . 5' BsaJl识别位点5 c'b NNGG.. 3J3； GGNNC/3,. 5BsaWl识别位点5 . ., w'bCGGW, 3J3•…WGGC即…hBsaXl识别位点5 . ^NjACfN^CTCCtN),；. 3-3r ..(N)TG(N)r GAGG(N). .，& 丄p ABseRl识别位点5…GAG GAG的「…3"3... CTCCTC{N)-A….5P BseYl识别位点5\, CV： CAGC.,. 3J CT…GGGTQ3…可Bsgl识别位点5；.. GTGCAG^N^T. 3J3 ….CACGTC{Nj i4i.. . 5JBsiEI识别位点5, . , CGRY'fcG,, 3r3 . .. GC A YRGC . ,5"BsiHKAI识别位点5；…GWGCW七■…J3：..C^WCGWG.. .5BsiWI识别位点5.C'feT ACG, . 3r 3：…GC A TGJS. 5’Bsll识别位点5' . , CCNNNN I^INGG. , .3r 3；., GGN\NNNNNCC.. ,5J BsmAI识别位点5r ^GTCTC(N)7 .3ri3 . .. C AG AG(N).…h▲BsmBI识别位点5. r,CGTCTC (N)J .3F3. ..GC AGAG (N)s^ ..5BsmFI识别位点覚…GGGAC(N)(D T . 3* 占…CCCTG(N),-A. . .5JBsmI识别位点5 . . . G A A T G C N* . . 3J3. . CT T AC^SN ..5JBsoBI识别位点5P. ..CVCG AG. .,3P3 . ^GRGCY^C . .5^Bsp1286l识别位点5 ….GDGC“b_・F 3〔…qHCGDG …5’BspCNI识别位点5\. CTCAG(N)/ . .3 3：…G AGTC (叽…BspDI识别位点5\ . A T T C G A T , , . 3"3：…T A G qj A「SBspEI识别位点5 …T T CCGGA. . ,3J3 . r AGGCCJ- . 5JBspHI识别位点5 ... T T C ATGA3’…A G T A CJ…翼BspMI识别位点5. ACC TGC (N)/. r. 33. .. TGG ACGBspQI识别位点5 . GCTCTTClNjJ .33LXGAGAAGtN)*^ r MiBsrBI识别位点5 . . . CCdtTC, . .3r3 GGC^pAG. . .5^BsrDI识别位点5；. . GC AATGNN*, .3r3 ：…C G T T A QN N …乳BsrFI识别位点5；, .R^ CGG Y . .. 3"3 ., YGGCC^R …5“BsrGI识别位点5 . lIST ACA .3…AC ATGJ…创BsrI识别位点5：.. ACTGGN T, r孑3：. . TG ACJDN .,. 5^BssHII识别位点5P.., G*CGCGC .. ,3P3.,.CGCGqp.,5^BssKI识别位点5 . . .'tCNGG . .3：.. GGNCq…hBssSI识别位点5 .(T A CGAG ,, .3"3F GTGCTJC .. .5^BstAPI识别位点5..GCANNNIS T NTGCCGT\NNNNACG8G5 阴BI识别位点5 . k. T T'b G A A t, 3J3. . AAGCJ T T5JBstEII识别位点5：.. G'fe T N A C C .. . 3^3 ... CCAhl TGJS.. .5^BstNI识别位点5：..cd\VGG. . . 3P3 . ..GGWCC . . 55：..CTCAG(N)tt^-专业资料分享BstUI识别位点5：.. CG'faG,. .3^3 . .. GCJ3C.. .5^BstXI识别位点5 ANNNNI S T N TGG.,3J3 . f.GGTN A NNNNNACC (5)BstYI识别位点5…R先ATdS3\, YCTAG^R.. .5JBstZ17l识别位点5…GT fT AC…卽3\. CA T^ATG.. .5-Bsu36l识别位点5.…CC?TN AGG. . . 3r3-…G G A N T& C. 5"Btgl识别位点5；,. C?bHYGG,. .33；, GG YRC^C , ,5JBtgZI识别位点5…GCGATG㈣「…3」3；..CGCTAC(N),4J...5,BtsCI识别位点5；. .GCAGTG N N T33：.. C G T C A (^N N ... 5'BtsI识别位点5.,,GC AGTG N N T.3P3；.. C G T C A <^N N ...Cac8l识别位点GCN T NGC . 3J3：…CGN/ICG…密ClaI识别位点ATfcGAT.. . 3J3 …T AGCJ A T. 5CspCI识别位点5 :的CAA (N>( GTGG (N)^.,33：・+严JN) GTT (M), CACC(N}^1V，,5J CviAII识别位点5…C T G…:T3 r.. G T A^C . . S PCviKI-1 识别位点5...RG T C Y (3)才…¥C#GR…5’CviQI识别位点S'-.J J TAC ,.3J3：5枣…5’Ddel识别位点5；-. C^TNAG.. .3J3；, G AN\C.Dpnl识别位点Ch,5 G A T C 3 3；. CT AG 5’DpnII识别位点5；r T GATC..33：r, CT AG^ . 5JDral识别位点5；.. T T T^AA …3’3\Rt A A A/TT . , 5JDralll 识别位点5；h CACNNN'bTG. . ,3“3…GT砂Nhl SC…5」Drdl识别位点5 . , GACNNNN T NWGTC, ,3 3：. . CTGNN^NNNNCAG., .5^Eael识别位点5“…gGCCR …;T3 . ..RCCGG/ .. 5JEael识别位点5, ^YfeGCCR, . 3J..RCCGG/ .Eagl识别位点5... C^SGCCG .专业资料分享3 .. GCCGGf. .Earl识别位点5 …CTCTTC (N)t\ .r3... GAGA AG (N)^. ..5PEcil识别位点5. . .GGCGGA(N)J . .3^GCCT (N)S A.., 5JEcoNI识别位点5： . . CCTNt/NNNAGG 33 . . GG ANNN^NNTCC . 5E CO O109I识别位点5P. . R(jfeNCC Y …:TS^.YCCNG/SR . . .5专业资料分享EcoP15l识别位点5 r C AGCAG(N)/ . P3r3 …GTCGTC(Wy 5EcoRI识别位点5 . ., A T T C ... 3 r 3；. . CTT AEcoRV识别位点5.GAT^TC -,3*3：.. CT AJ AG.. .5JFatl识别位点5：・先ATG…3」3\t GTAC^ .. 5’Faul识别位点5J CCCGC (N}J.. .3' 3；.. GGGCG (N)#A. . ,5J Fnu4HI识别位点昭…GC’NGC…朝3... CGN&G -SFokl识别位点5\L.GGATG(N)；1T3J3... CCTACtN),^ .5Fsel识别位点5 …GGCCGG'tc, .33 . CC/3GCCGG . . 5J Fspl识别位点5；,. TGC^bC A .. .3P3 ； ,. A C GJC G T (5)Haell识别位点5 RGCGC T Y. . .33 …LCGCGR …5，HaelllHgal识别位点5 . . .GACGC^N}/. 3... CTGCG<N}1(W. . 5" Hhal识别位点5 …GCdt …3" 宙…中CG…5，Hindi识别位点5：f GT Y T R AC.. .3r3\ .. CAR A YTG fHin dlll 识别位点5；. , A'A G C T T .…3" 3... TTCGA^A., .5JHi nfl识别位点5’ …GklMTC …3 3\., CT NAJG., .5* HinPII识别位点亦…dtGC…:r3 ... CGCp . . 5* Hpal识别位点5. .. GTT> AC.. .T3.., C A AJTG. _5J Hpall识别位点5“...cffeGG (3)3. GGC A C. 5JHphl识别位点51 …GGTGA (N)/.,.3, 3；.. CC AC T (N)7i,., 5" Hpy188l识别位点5 …T G “G A「3A0J CT …£Hpy188lll识别位点5.,TtfNNGA. ..3^3.,AGNN^CT. , ,5JHpy99l识别位点5；.. CGWCG T3：F i GCWGC.. ,5^HpyA V识别位点5.h CCTTC(N)J,3-3：. GGAAG(Nk ...5*%HpyCH4III识别位点h…A C N’G T …3"3 .,. T G^N C A … .5 HpyCH4IVHpyCH4Vm别位点5：. . TC?t A ,. .33■…A QG T…5」Kasl识别位点5.., G^J CGCC.,3P3r.^CCGCG^,..5J Kpnl识别位点5.,, GGT AC't... 3^3■…QCATGG …5，Mbol识专业资料分享别位点5P. .^3ATC .3^3P.. CT AG^. .5^Mboll识别位点专业资料分享5；., GA AGA (N「…3* 3；,, C T T C T (N)J A，. .5* Mfel识别位点5；.. C'X ATTG. ..3^3；T . GTT A Ajp . . 5JMlul识别位点5... /T CGCGT.,，3r3 ：.. T G C G C^A ... 5JMlyl识别位点5 …GAGTC(N, 一33\. .CTCA G (N) . 5-Mmel识别位点5； . .TCC RAC(N)M T.3' 3： . ,AG G YTG(NJ ia>. ..5*MnII识别位点5 ….CC「C (N}/ . 3 3…GGAG㈣立,5-Mscl识别位点5 , F TG(?fc：C A t,3P 3"…ACC^J GT.+J5JMsel识别位点5... f*T A A.. 3P3；T. A A TJ .. . 5^Msll识别位点5 …CAYNNV IN RYG. .,3J3 ...GTRNN^NNYAC (5)MspAII识别位点5P. . . CMCSfcKG, . .3 3•…GKYMC…扩Mspl识别位点5；F. dfcGG …3’3；. . GGqp …5’Mwol识别位点5 . . G C N N N N NVj N G C.. . 3\_ CGNN^NNN NNCG., Nael识别位点5 ... GCC\3GC,. .3r 3：…GGG&GG-.hNarl识别位点5 …GG^t GCC.. .3 3…CCGQGG …5“Ncil识别位点5 .C(7SGG,. ,3r 肚…GGS&C…・h Ncol识别位点5. ,,C T CATGG,. .3*3\ ,. G G T A CJ3 ... 5JNdel识别位点5； ., C /T T A T G ,. 3^ 3... GT A\AC…hNgoMlV识别位点5 . _ (itCGGC,. 3^ 3•…CGGCC^G. . 5Nhel识别位点5 . • T AGO . .,3"3 …GGATqp …hNlalll 识别位点亍…CATCf…3’AG…hNlalV识别位点5\,. GGt/NCC-.3 .. CCN^NGG.. .5JNmeAlll识别位点5：.. GCCGAG{N—G 3 ...CGGCTC(N)ia.^.^5rNotl识别位点5〔GdtGCCGC …;r3 ...CGCCGtyCG (5)Nrul识别位点5 . BT TCG^GA …3J3 …MCFCT …hNsil识别位点5 .ATGC^T T .. .3^ 3... I^ACGTA .. ,5JNspl识别位点5 . RCATG T Y. . 3J 3；.，X h GTACR.,.5rPacl识别位点5...TTAAf r TAA...3-3：+ , A ATJ A ATT T J+5JPaeR7l识别位点专业资料分享5；.. CG AG . ,3P. +G AGCTJC , . 5JPcil识别位点5：…A%島TGT….3*3* . . TGT A CA.. .S,APfIFl识别位点5；, .GAChiVlNGTC .…扩3 . . . C T G N N A N C A G ... 5* PflMI识别位点5； .. C C A N N N wTl T G G.. . 3^ 3；^GGTN A NNNNACC., .5^ Phol识别位点5：...GGfcC (3)3：. CCjjG.. 5JPlel识别位点5\.. GAGTC .3* 3：. CTCAG (N),,. hMiPmel识别位点5：. GTTT^AAC. . .3J3；.T C AAAJ T TG.T 5JPmll识别位点5；,, C AC^TG.. .33P.. GTGJ：AC- .5HPpuMI识别位点5 , h RdTj W c C Y ・”，3“3；_ YCC WGJ3R ., 5J PshAI识别位点5；ACNl7NNGTC . +.3J3 , . .CTGNNJNNC AG . .5'Psil识别位点5；., TT^T A A…X .. A A T A T T ... S HJLPspGl识别位点5 …1tC WGG …3“3；r,GG WCC^ ,.5PspOMI识别位点5；,. (fGGCCC.,. 33：…CCCGGJ3…5’PspXl识别位点5 V(7T CGAGB . b3J3. 8GAGC\CV.. .5*Pstl识别位点5 …CTGC 屆.33；. . G^ACGTC-. S-Pvul识别位点5 …CG ATEG …3" 3；.. GCJ AGC. . . 5JPvull识别位点5；…CAtSfcTG…富. . G T CJ3 A C ... 5^Rsal识别位点5...GT^C (3)3... CAJG.. .5*Rsrll识别位点5；, ,C(?fewCCG.. .3' 3：…GGC W枣C…hSacl识别位点5 GAGCT^ ,3J3；CJCGAG, . 5JSacll识别位点热…CCGC七G 23 .. GGjpGCC . 5JSall识别位点5 G T TCGAC,. 3J 3…CAGCT^S …hSapl识别位点5； .-GCTCTTCtN)^ ,3J 3 . r,CGAGAAG(N)(j.TJ5JSau3AI识别位点5 .. 7G A T c .,. 3^ 3：.. CT AG^..5JSau96l识别位点5... G^SNCC . . 33 …CCNGa ・h专业资料分享Sbfl识别位点TGCA T GG.,y3.., GG/CGTCC , 5’Seal识别位点5：.. A G T鼻C T ...甞3\,. TC AJGA . _5JScrFI识别位点5 ... CCT N GG.. 33\. GGNJSC , . 5JSexAl识别位点5 , .rfbCWGGT., ,3r3 …TGGWCqA. - SSfaNI识别位点5.., GC ATC (N)/ ,.. 3*3J.,. CGT AG (N)9A，.. 5*Sfcl识别位点5：…(?TR¥AG …;T3：. .GAYRT^C. . ,5Sfil识别位点专业资料分享5…GGCCNNNhTNGGCC .,3 3. ,CCGGN/tNNNCCGG .5'Sfol识别位点5；., GGC^CC ... 3J 3：…CCGfGG …5，SgrAI识别位点5 …C R’C CG G ¥ G …3"3. ,.G YGGCC jt AC,,,5HSmal识别位点5；., CCC^GG. . 3r3 …GEGfCC 一5"Smll识别位点S^.C T T Y R AG (3)3"…GARY .C …/SnaBI识别位点5 …T Ad A …3’3P r. A T G^： A T .. S HSpel识别位点5；. . zTCJ AGT . ,3r 3：…TGATC^A …hSphl识别位点5.GCATG'fe.. 3J3... CpT ACG . 5Sspl识别位点5 . A AT^TT 3P3. . . T T AJ AA . .5*Stul识别位点5. R1AGdtCT . ,.3f 3：…TCCJSGA. .5HStyD4l识别位点5 .. T CCNGG.,.『3’…GGNCq…5“Styl识别位点5仁C T CWWG G.. ,3r3；, GGWWC A C..Swal识别位点5 . , , A T T TV A A T ・・33 . h. T A A A J T T A ... 5^TaqaI识别位点5：.. T^G A . . ,33：…AGCJ ., 5JTlil识别位点5... <T T CGAG,,. 3J 3…GAGC"…5"Tsel识别位点5 …gfcwGC …3，3 …CGWQG …Tsp45l识别位点5；, ^STS AC.. 33\. .C ASTG^ . 5JTsp509l识别位点5….V A T T… 3-于…TT A. 5“TspMI识别位点C T CCGGG…3’3’…GGGCCf •…5’TspRI识别位点5 .NNCASTGNN T. ,3J3 .. ^NNGTSACNN., .5*Tth111l识别位点5P. . .GACN T NNGTC..3P3'…GTGN 叫NGAG-hXbal识别位点ftT AGA .3’…A G A T GJ…亍Xcml识别位点ANNNN N T J NNNTGG. 33. . .GGTNN N N^NNNNNACC. . 6Xhol识别位点占…C T TCGAG- 33 …GAGC T& …5"Xmal识别位点专业资料分享5：…CtCGGG…計3；.. GGGCqC . . .5* Xmnl识别位点5..,GA ANNVlN T TC . . 3r 3\ .. C T T N NJM N A A G .,. Zral识别位点5：. ,G AC T GTC■…孑3；, CTG A CAG...5-Tfil识别位点5 , . .G^WTC., .3 3 , . XT W .5。
限制性内切酶酶切位点汇总
Alel 识别位点Aatll 识别位点 5「・GACGTt ・・”3‘ G …CJGCAG …却Acc65l 识别位点5 _ GrtaKAC - 31 3：..CAKMJG..,5Accl 识别位点5. .-GfSnCAC .T.Sl3：..CAKMJG (5)Acil 识别位点S. - .C'feGC, ..3 3： ..GGCG. ,.5JAcll 识别位点$…从二 3“p ： .. TTGC/A .. .5'Acul 识别位点5： , XTG A AG(N)…\. . 3 p\. GACTTC<N}^ ・■ 5Afel 识别位点B -,AGC^JCT .. .3 忙…TCqp" . 5Aflll 识别位点5 ,.(TTI^AC,r.3-3：「G A A T 1T& (5)Afllll 识别位点吕…总RW.3UGgqA (5)Agel 识别位点&'、, Q GGGGT …E3： . . TGGCCi t A...5,Ahdl 识别位点6 . G ACNN L T NN GTC ,r 3 3： .XTGNb<NNNCAG. -515 . ..CACNrTNNGTG- ..33 ..GTGNN^hlNCAC . 5^Alul 识别位点5： .. AGtT ..*3 3.. . TCpA...5JAlwl 识别位点5 ..GGATC<N )7 . 3' 3： .. CC7 AG{N)，A . . 6HAlwNl 识别位点rT7CAGNNfftTG77.3" 3J ...GTq fc NNNGAC....5JApal 识别位点5：…GGGC<?t ,…h 3.. XpCGGG...5^ApaLl 识别位点$…密TGCAC …了 3」…CAGjG …5’ApeKl 识别位点5：…GSGC …3 扩…百GWGQ …护Apol 识别位点V — FTAAT T ¥ _ _3" 計…■节TTAAf …hAscl 识别位点5 …GGtGCGg …3" 3. . CCGC3CJ3G . 5JAsel 识别位点5 …A TT A AT …3" 扩…TA A7JAAsiSl 识别位点5 …GCGAffcGC.. 3 CGqr的CG .亍Aval 识别位点5 …CT¥CGRG,・3 3：…GHGCWC …5「Bcgi识别位点5. ..\/N)CGA{N>fl TGC(N)17，丁霞.十㈣GCT㈣』C®叭苫■ ■BciVI识别位点5；.. GTA TCC(Nj T33. .,CAT AGG(N}^. .5'Bcli识别位点5’…ft ATCA ■一3「3：.. ACTAGJ.…5JBamHI Bfai识别位点5：・.(Tr AG …丁3：”. G負咚；…亍BfuAI识别位点5：. ACCTGC(N>7 T3：…TGG ACG(N}g .hBgli识别位点5；. GCCMNhN^lGGC., ,3^3：. CGG^NhNNCCG 5Bglii识别位点5；. A^3 ATCT tt +3*3；.. TCTAi^A .. .5^Blpi识别位点5 …GgTNAGC …3"3：.. CGANT^CG .5"Bme1580l识别位点亍…GKGCWfc…;T扩…qMCGKG.. 5"BmgBI识别位点5：,.CAC?feTC.^3 扩…GTqfAG・5"Bmri识别位点5 ACTGGG(N)3T3"3. .. TGACCC(N)^ 5Bmti识别位点5；. ,GCTACrC,..3^3：. .CGATCG.-.5^Avail识别位点&…富...心CWGf (5)Avril识别位点比TAGG…旷P：..GGATCJD...5'Baei识别位点5； ^.^NlACfN^GTAYCfM)^ 一3”3 =JN)TG(NhCATRG(N)『..5“识别位点6.. .G^ATCC...3HOCTAQp…SBanl识别位点S：^G'bYRCC^,3-3.. .CCRY(\G...6*Ban II识别位点KRGCe …L y..C"GRG …亍Bbsi识别位点|5：.G A AG AC…:TB. …CT TCTG (Nk …弓-BbvCI识别位点H…CC T TCAGC…劭扩…右G由GLUG…亍Bbvl识别位点S P. .GCAGC<N)7 . ,33. . .CGT€G^N)l?i..5rBcci识别位点5 ATC(N)7 .3一卜-.GGTAG叫・hBceAI识别位点，…ACGGU(M)二3 3「.TGCCG(N)鉴「5”Bpml识别位点|5： .CTGG AG{N)V T.3^ p： ..G ACCTC(N)1Jk. .5 BpulOI识别位点5 …ccTrzGC …w GGANl^CG …引BpuEl识别位点p：C TTG AG(N)/. . r3*p. ..GAACTC(M)v^.t5-BsaAl识别位点t；YAC^GT A , 3P：.,RT(\CA? .5^BsaBI识别位点5： . .GATNN'klNATC . 3'p j. XTANN a NNTAG * hBsaHI识别位点亍…GhtGM…丁S^.CYGC^RG. -5JBsal识别位点B：. G G T C T C (肝,.3 p：. . CCAGAG |叽…hBsaJI识别位点5J. ..C'tNNGG.. 3p... GGNNC^. .5BsaWI识别位点b.「W\；UGG 內.・.3一WGGCC^W 6BsaXI识别位点p：\(N)AC (N)&CTCC («)f¥3’3 . rj(NHG(N)" GAGG(M). .BseRI识别位点5 .GAGG AG(N|/ T. . C TCCTC (N)q』…亍BseYI识别位点5 .X\：QAGC---3fl乳. £GGTqp…宙BsgI识别位点5. ..GTGC AG(N)16T.3^ 3 . .«C ACGTCtM),^ . ,5H BsiEI识别位点GGRA T G,3”3’…GCJFIGC；…5’BsiHKAI识别位点5：・.GWGCW七…古a^.^WCGWG ...5"BsiWI识别位点「庞TZG；…3"3’…右CA TG$ 5"BslI识别位点5J . CCNNNNlThJNGG . 3* 3r . GGNN^NNNNNCC …5" BsmAI识别位点5：GTCTC(NjJ 3"3. .. C AG AG (⑷脣…亍BsmBI识别位点5 ..CGTCTC (N)/ , 33 . ..GCAGAG (NK k.. 6' BsmFI识别位点5 …GGGAC^N)./. -3 驭…CCC1GR1-A..5 BsmI识别位点5. G AATQCM T3"3：CTTACGN 亍■BsoBI识别位点5： ..CYMRG ~3"3. ..GRGCY^C .5^Bsp1286l识别位点5】・「G D G C (3)3：…QHGGDG .…5’BspCNI识别位点and BssKI 识别位点BtgI 识别位点5： . ,CTC AG(N}J .33 . .G AGTC (N)s - .3 5…1tCMG …旷3.. GGNCq...5JBssSI 识别位点5 …疗2GAG •，丁 3JGTGG 即…亍BstAPI 识别位点5 .,GCAIMMN hfN TGC ...3 3.. .CGT\NNNNACG. (5)BstBI 识别位点5…TT 七GAA …3^ Y …AAGQTT …hBstEII 识别位点5r Tt l?feTNACC 3\,CGANTG j G r ..5*BstNI 识别位点5 …CtfwGG …丁 3. GG\\CC. 5BstUI 识别位点亍…CGtG, 33’…cqpc …5’BstXI 识别位点5., ,CCANNNNN T NTG G 77,33； GGTN^NNNNNACC £BstYI 识别位点5：「RST£¥ …3"YOTAQP …国BstZ17l 识别位点5. h f AC …3 3：.XAT ；ATG,..51Bsu36l 识别位点5 ...NAGG (3)3；..GGANT A OC.. 5BspDI 识别位点5；, . AffeG AT .3*.. . TAGCJA . ..5fBspEI 识别位点畀一代CGG 山■…丁3：.. AGGCCJ^.5^BspHI 识别位点|511T ftATGA.,3' P ：.. AGTACJ. . .51BspMI 识别位点6 . ACC7GC (N)J. .y r. .. TGGACG Bhj , SBspQI 识别位点fr ：..GCTOTTC(N^..T S\a .QQAGAAG^J .S ,BsrBI 识别位点5； ~CC^V T C,-3- 3J ...GGC^AG.-S^BsrDI 识别位点|5-.. GC A ATGNN^,.^* 卜…CGTTAqNN …5」BsrFI 识别位点AtCGG”…3" YGGCC^R ^5BsrGI 识别位点5 ：, UtTACA r -3" 3：.. AGATGJ. ,5JBsrI 识别位点S AC TGG N T. .3*3： . . TG ACf N …5，BssHII 识别位点5 VCGCGC., .3 J ,Dpnl 识别位点3： ..GGYRC^C ,5JBtgZI 识别位点i5P .GCGATG(N)/ 3* p ： ..CGCTAC(N),^ 5「BtsCI 识别位点fc ； CAG TG NN T,, 3 &\.CGTC ACNN..,5JBtsI 识别位点|5； ,T G CAG TG NN T,, J b, ,.CGTC AGNN r .,5JdkCac8l 识别位点GCN^GC.^3 扩…CGI^NCGClal 识别位点6；.. A T\3 GAT -.3^ 扩…TAGCJ A..SCspCI 识别位点5： 一 \n (N) CAA (N), G TGG(H)1；| 二3* ^.u^GTT^CACC^^^, 5^CviAII 识别位点fc^.C'XTG , . b 3* 卜…GTAf …hCviKI-1 识别位点5…R<TC¥ 一3 3"…¥GJ3Fl 一5CviQI 识别位点5：. <fTAC^,3' 孑...CATf (5)Ddel 识别位点5： — CfTfrJ AG.…：T 扩…昨冉蝌T 卫…玄CH, I ；5 G A T C ,33： CT AG 5"A lCH 3DpnII 识别位点5… $ AT 匚…丁 3；., CT AG a .. .5,DraI 识别位点煮「TT T% AR …了3\t AAAJTT ,.-5JDraIII 识别位点5 …CACNNN 力 TG …丁 勞…GTG&NM 忖CAG-眷DrdI 识别位点5 . ,.GAC MN MlAlNQTC, ,,3 3.. .CTGNN i NNNNCAG (5)EaeI 识别位点5 …ZbXCR …3“ 3.. .RCCGG^ . 5JEaeI 识别位点6；^/bGCCR...3J 3.. .RCCGG^.. 5JEagI 识别位点亍…西GGGG,…丁 扩…GCCGqp …5’EarI 识别位点5 ^CTCTTC (N) T …3 3. GAG A AG (N)^ .5EciI 识别位点5 \ GGCGGA^N^^ …3"3•…CCGCC"叽…5"EcoNI 识别位点5 …CCTN 枷 NNAGG …3"3 . . GG ANNNJM NTCC .,5' EcoO109I识别位点Hgal识别位点9：YCCNG^R.-S^ EcoP15l识别位点5 H CAGCAG(N)/ y 3\..GTCGTC(N)a^. (5)EcoRI识别位点5；, ,<?A AT TC ,3^ 扩…CTTA Af…亍EcoRV识别位点帀GAT^TOP^.CT AJ AG (5)Fatl识别位点5：・N A TG (3)3 .. GTAC iL..5JFaul识别位点IS CCCGC ... 3* A「GGGCG(N)蛀…亍Fnu4HI识别位点F …GbNGC：…3" 护…CG M&G…5"Fokl识别位点.. G G A TG(N)/ 一3 p J. . . CCT AC(N),^ , .5' Fsel识别位点■….GGCCGCTbC；…•才a：.. CGpGCCGG . 5^ Fspl识别位点TG氏C—聞3：.. AC<^pGT...,5Haell识别位点5 …RGCGC TV…：r 扩…YpGGGR-仔5 .G ACGC (忖3： .,CTGCG^N}^ . 5r Hhal识别位点5 …GCGt：, ..3P 3：..CpCG..k5^Hin cll识别位点5…GT 沖2・33：…C為堂TG —hHin dlll 识别位点5…/C A GCTT・・・3「孑…T …hHi nfl识别位点5…G^NTC …b3’..."N比G (5)HinPII识别位点5, ..Gt GO ・・3 3：…CGCQ …hHpal识别位点亍…G* Tk AC…3’3.. .CAAJTG...5^Hpall识别位点5 ….C^GG , . .3' 扩…GgG…中Hphl识别位点5 GGTGA (呵「…3’31…X ACT叽…hHpy188l识别位点新...T C『G A (3)3 .., AGjlCT (5)Hpy188lll识别位点5.. r7CTNNGA...3H 計…典GNNfT-hHpy99l识别位点5r. r r CGWCG T -3'S-.^GCWGC. ,.5JHaelllMnII 识别位点HpyAV 识别位点S ：.,CCTTC(N)/,.,3, 3\ GGA AG{N)^ . 5^HpyCH4III 识别位点|5*.,. ACN^ST ...M 卜...T(^N C A (5)HpyCH4IV HpyCH4V 识别位点出…3“人砂丁…hKasl 识别位点亍…翻CGCC., 丁 缶…CCGC<\p …hKpnl 识别位点6. ..GGT AC^T. ,3' 吿…qpATdG …刖Mbol 识别位点乔0ATC …3” 卜…GTg ,.5"Mboll 识别位点|5■…GAAGA (N)J ,3 忙…CTTCT (N)“ _SMfel 识别位点C ATTG …丁 3…GYTAAJ3…柠Mlul 识别位点y.fCGCGT 3 3J ^TGCG^A., 51Mlyl 识别位点5 . G AGTC{NJ7 3' 3 . GTCAG (NL ・.EMmel 识别位点h …TCC RAC (州广…3" p ； . . AGGYTG(N)tir ..5J 5r . CCTC (N)J 3 扩…GGAG ㈣;Mscl 识别位点 5 TGC^CCA.,,3*3：.. ACCpGT...5JMsel 识别位点5"…TP AA …丁 3. h . A AT^T 5FMsll 识别位点S ;.1CAYNN 1F MNRTG ^.3^3r .. CTRhll^NN YAC hMspAll 识别位点5 .. T CM<St KG ,…3’ 茁…GKqp 衲C..6Mspl 识别位点5.. .C?bGG.,,3^ 扩…GGqC ….5’Mwol 识别位点5.. .GCNNNNN*NNGC ・_3 3r . CGNN^NNNNNCG .5"Nael 识别位点5…GC 祠GC …3’ 3：..CGG b CCG...5-Narl 识别位点亍,G 比GGC …3^ccotyse (x)Ncil 识别位点亍…CC*SGG …;r3 .. .GGS^CC. .5*Ncol 识别位点亍…牝ATGG …聞 扩…GGTACQ …SNdel 识别位点C/fT ATG …了 3：..GT AT ；AC …5’NgoMlV 识别位点6：..<?tCGGC ••卫ET GGG右於…5」Nhel识别位点6："Gt TAGC...3" 缶…CGATQG…hNlalll 识别位点S. ..CATC T ..aNlalV识别位点$p GGEg…y3：.. GCh|b NGG...5JNmeAlll识别位点5^GCCGAG(N)SM*-.3,gGQTC(N)s ・ FNotl识别位点吕….GCtGCCGC,「歹工…©右CCGGjf右…亍Nrul识别位点y…TCCftG島…丁3.. . AGCpGT .月Nsil识别位点杖…ATGG肝「丁3.. . V CQT* -5JNspl识别位点$…RCAT(?¥…旷p\ ..^GTACR . .5^Pacl识别位点砧…TTAA F T片山『3 ... A A T J A A T T (5)PaeR7l识别位点5\r^TCGAG r3'3\ GAGCT b C (5)Pcil识别位点$…心AT GT ■二① 卜…TG"qA.. E PfIFI识别位点^-.GAGhTNNQTC ...3*a^.CTGNN^NCAG (5)PfIMl识别位点5；ANNN^NTGG ・・，3 3 ^GGTN A NNhlNACC..,5Phol识别位点5：・・GGtC…孑Y…CCQD…弓Plel识别位点6 . . GAGTC (NjJ 33「…GTCAG ㈣.…5’Pmel识别位点5 …GTTT% 応AG …3“3…GA A AJT TG…弓Pmll识别位点S .-.CAC'GTC .•同3J ..GTG^AC …亍PpuMl识别位点Y...¥CC WQGB (6)PshAl识别位点5；..GACNN T NNGTC, ..a* 3 …CTGNN』NC2.-£Psil识别位点5 二・TTfT八WATT.•同PspGl识别位点5：. ECWGG -糾3’…GGWgSPspOMI识别位点&,.,<fGGCCC7.,3J.CCGGqp-. +5'PspXl识别位点Sbfl 识别位点3^ .GG/CGTCC 5^Seal 识别位点5 .. T AGrtCT , ir 3' 3.. . TCAJGA ...5^ScrFl 识别位点5…“NGG …3 3：..GGy SSexAl 识别位点5,—XtCWGGT?.^' 3：..TGGWCqA (5)SfaNl 识别位点5 .. GCATC (N)J. .3-GGTAG (N)^...5_Sfcl 识别位点5 …(7TR¥AG …3" 3r .. G AYR*QC .. .5"Sfil 识别位点5 …GGCCMN N EGGCC …3" 3 .CCGGI^NNNNCCGG 5JSfol 识别位点WGG 显CC …3” 3「CgCGG …5」SgrAl 识别位点亍…Crft GGGg.33' .GYGGCC^RC . 5*Smal 识别位点TCC^&GG ?3 ..GGGpCC.. 5'Smll 识别位点亍…brifRAG …暫3'. . ,G A R Y T ；C, ..5FSnaBl 识别位点5二・TAC^GTA ・・・3 3J . ..A TGC AT... S'&…VtfTCGAGB3F .BGAGCT^CV r 5^Pstl 识别位点F …CTGC R …：rIT …qkUGTC . 5’Pvul 识别位点・.一 CGATtG.「孑3.. -GCJ AG J .5*Pvull 识别位点15, , CA<?tTG. ,.3 P ： . GTCpAC.,. 5^Rsal 识别位点F ・・・GTN C ,「才3\.CAJG..5JRsrll 识别位点b …GGCWCipC …中Sacl 识别位点S ： ..G AGCT'fc., .3 扩…C.TCG 占G.. SSacll 识别位点S ；,.CCGtfbG.,.3 3 . ..GGJ3GCC . , .5JSall 识别位点$…G T TCGAC …:rp J ...CAGCT (i G (5)Sapl 识别位点$…GCTUT TC 慣卓3(3. ,,CGAGAAG(N)Jk .,5^Sau3AI 识别位点5 .. 7c A T c - 3^ S- .-CTAG^ . .5*Sau96l 识别位点S^-GlSNCC-. ,3rSpel识别位点6；../fCTAGT, r.3'TGATG/ .-S^ Sphl识别位点6；.. G C A T dt...3^CjST ACG …5 5.. ^TS AC.. .3r Tsp509l识别位点5’…1r T …丁雷…T T A A^. . 5H TspMl识别位点5\. C*CCGGG 3…GGGCC&Sspl识别位点6：,. AA T工TT …N 缶…TT AJAA . ..5J Stul识别位点TspRl识别位点5…村MCASTGN3 3.,^MGTSACNN-..5^ Tthllll识别位点StyD4l识别位点5..,GACN T NNGTC rrT3" S.-XTONN^NCAG. ..5'$：”乂CNGG (3)3：..GGNCq..6Styl识别位点WCWWGG …3,3 +. .6 G WWC^C 一5「Swal识别位点5 ：.. A T T A A T ,.. 3 3：.. T A AAJ TT A.. 5 Taq aI识别位点S…让GA…$3：.. AGCJ (5)Tfil识别位点5： ,,G^W rc ,,3 3...CTWAJ3...5"Tlil识别位点6：T.C T CGAG-7,3*3R .GAGCTJ3 . 5^Tsel识别位点•・・G'bWG£・*3"a p ^CGWCjG …5 Xbal识别位点5 …ffcT AGA …3“3... AGA TC^T . ..5'Xcml识别位点5, ANNNNN'NNNNTGG...3,3r GGT NNNh^NN NN NACC. . 5^ Xhol识别位点5“…疗TCGAG…可31…G負GC“ …亍Xmal识别位点煮…比QGGG…;T 勞…GGGCqC…5’Xmnl识别位点5.1+GAANN^MTTC .…3"3：. XT TN N^N MA AG .. 5"Zral识别位点h…G現龙TC…①3：. CTGjGAG--. 5'Tsp45l识别位点。
史上最全限制性内切酶酶切位点汇总情况
A系列AatII识别位点Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI 识别位点BanI识别位点BanII识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I 识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。
限制性内切酶酶切位点汇总
RsaI 识别位点 RsrII 识别位点 SacI 识别位点 SacII 识别位点 SalI 识别位点 SapI 识别位点 Sau3AI 识别位点 Sau96I 识别位点 SbfI 识别位点 ScaI 识别位点 ScrFI 识别位点 SexAI 识别位点 SfaNI 识别位点 SfcI 识别位点 SfiI 识别位点
AatII 识别位点 Acc65I 识别位点 AccI 识别位点 AciI 识别位点 AclI 识别位点 AcuI 识别位点 AfeI 识别位点 AflII 识别位点 AflIII 识别位点 AgeI 识别位点 AhdI 识别位点 AleI 识别位点 AluI 识别位点 AlwI 识别位点 AlwNI 识别位点
BstUI 识别位点 BstXI 识别位点 BstYI 识别位点 BstZ17I 识别位点 Bsu36I 识别位点 BtgI 识别位点 BtgZI 识别位点 BtsCI 识别位点 BtsI 识别位点 Cac8I 识别位点
ClaI 识别位点 CspCI 识别位点 CviAII 识别位点 CviKI-1 识别位点 CviQI 识别位点
ApaI 识别位点 ApaLI 识别位点 ApeKI 识别位点 ApoI 识别位点 AscI 识别位点 AseI 识别位点 AsiSI 识别位点 AvaI 识别位点 AvaII 识别位点 AvrII 识别位点 BaeI 识别位点 BamHI 识别位点 BanI 识别位点 BanII 识别位点
BbsI 识别位点 BbvCI 识别位点 BbvI 识别位点 BccI 识别位点 BceAI 识别位点 BcgI 识别位点 BciVI 识别位点 BclI 识别位点 BfaI 识别位点 BfuAI 识别位点 BglI 识别位点 BglII 识别位点 BlpI 识别位点 Bme1580I 识别位点 BmgBI 识别位点
酶切位点汇总
B yPe t e r XuAcc65I 识别位点AccI 识别位点AciI 识别位点AclI 识别位点AcuI 识别位点AfeI 识别位点AflII 识别位点AflIII 识别位点AgeI 识别位点AhdI 识别位点AleI 识别位点AluI 识别位点AlwI 识别位点AlwNI 识别位点ApaI 识别位点ApaLI 识别位点ApeKI 识别位点ApoI 识别位点AscI 识别位点AseI 识别位点AsiSI 识别位点AvaI 识别位点AvaII 识别位点AvrII 识别位点BaeI 识别位点BamHI 识别位点BanI 识别位点BanII 识别位点B yPe t e r XuBbvCI 识别位点BbvI 识别位点BccI 识别位点BceAI 识别位点BcgI 识别位点BciVI 识别位点BclI 识别位点BfaI 识别位点BfuAI 识别位点BglI 识别位点BglII 识别位点BlpI 识别位点Bme1580I 识别位点BmgBI 识别位点BmrI 识别位点BmtI 识别位点BpmI 识别位点Bpu10I 识别位点BpuEI 识别位点BsaAI 识别位点BsaBI 识别位点BsaHI 识别位点BsaI 识别位点BsaJI 识别位点BsaWI 识别位点BsaXI 识别位点BseRI 识别位点BseYI 识别位点B yPe t e r XuBsiEI 识别位点BsiHKAI 识别位点BsiWI 识别位点BslI 识别位点BsmAI 识别位点BsmBI 识别位点BsmFI 识别位点BsmI 识别位点BsoBI 识别位点Bsp1286I 识别位点BspCNI 识别位点BspDI 识别位点BspEI 识别位点BspHI 识别位点BspMI 识别位点BspQI 识别位点BsrBI 识别位点BsrDI 识别位点BsrFI 识别位点BsrGI 识别位点BsrI 识别位点BssHII 识别位点BssKI 识别位点BssSI 识别位点BstAPI 识别位点BstBI 识别位点BstEII 识别位点BstNI 识别位点B yPe t e r XuBstXI 识别位点BstYI 识别位点BstZ17I 识别位点Bsu36I 识别位点BtgI 识别位点BtgZI 识别位点BtsCI 识别位点BtsI 识别位点Cac8I 识别位点ClaI 识别位点CspCI 识别位点CviAII 识别位点CviKI-1识别位点CviQI 识别位点DdeI 识别位点DpnI 识别位点DpnII 识别位点DraI 识别位点DraIII 识别位点DrdI 识别位点EaeI 识别位点EaeI 识别位点EagI 识别位点EarI 识别位点EciI 识别位点EcoNI 识别位点EcoO109I 识别位点B yPe t e r XuEcoP15I 识别位点EcoRI 识别位点EcoRV 识别位点FatI 识别位点FauI 识别位点Fnu4HI 识别位点FokI 识别位点FseI 识别位点FspI 识别位点HaeII 识别位点HaeIIIHgaI 识别位点HhaI 识别位点HincII 识别位点HindIII 识别位点HinfI 识别位点HinP1I 识别位点HpaI 识别位点HpaII 识别位点HphI 识别位点Hpy188I 识别位点Hpy188III 识别位点Hpy99I 识别位点HpyAV 识别位点HpyCH4III 识别位点HpyCH4IVHpyCH4V 识别位点KasI 识别位点KpnI 识别位点MboI 识别位点MboII 识别位点B yPe t e r XuMfeI 识别位点MluI 识别位点MlyI 识别位点MmeI 识别位点MnlI 识别位点MscI 识别位点MseI 识别位点MslI 识别位点MspA1I 识别位点MspI 识别位点MwoI 识别位点NaeI 识别位点NarI 识别位点NciI 识别位点NdeI 识别位点NgoMIV 识别位点NheI 识别位点NlaIII 识别位点NlaIV 识别位点NmeAIII 识别位点NotI 识别位点NruI 识别位点NsiI 识别位点NspI 识别位点PacI 识别位点PaeR7I 识别位点PciI 识别位点PflFI 识别位点B yPe t e r XuPflMI 识别位点PhoI 识别位点PleI 识别位点PmeI 识别位点PmlI 识别位点PpuMI 识别位点PshAI 识别位点PsiI 识别位点PspGI 识别位点PspOMI 识别位点PspXI 识别位点PstI 识别位点PvuI 识别位点PvuII 识别位点RsrII 识别位点SacI 识别位点SacII 识别位点SalI 识别位点SapI 识别位点Sau3AI 识别位点Sau96I 识别位点SbfI 识别位点ScaI 识别位点ScrFI 识别位点SexAI 识别位点SfaNI 识别位点SfcI 识别位点SfiI 识别位点B yPe t e r XuSfoI 识别位点TseI 识别位点SgrAI 识别位点Tsp45I 识别位点SmaI 识别位点Tsp509I 识别位点SmlI 识别位点TspMI 识别位点SnaBI 识别位点TspRI 识别位点SpeI 识别位点Tth111I 识别位点SphI 识别位点XbaI 识别位点SspI 识别位点XcmI 识别位点StuI 识别位点XhoI 识别位点StyD4I 识别位点XmaI 识别位点StyI 识别位点XmnI 识别位点SwaI 识别位点ZraI 识别位点Taq αI 识别位点TfiI 识别位点。
限制性内切酶酶切位点汇总(优选材料)
AatII识别位点Acc65I识别位点AccI识别位点AciI 识别位点AclI 识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII 识别位点AgeI 识别位点AhdI识别位点AleI识别位点AluI 识别位点AlwI识别位点AlwNI 识别位点ApaI 识别位点ApaLI 识别位点ApeKI 识别位点ApoI 识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI 识别位点AvaII 识别位点AvrII识别位点BaeI识别位点BamHI识别位点BanI识别位点BanII识别位点BbsI识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BspCNI识别位点BseRI 识别位点BspDI识别位点BseYI 识别位点BspEI识别位点BsgI识别位点BspHI识别位点BsiEI 识别位点BspMI识别位点BsiHKAI识别位点BspQI识别位点BsiWI识别位点BsrBI 识别位点BslI 识别位点BsrDI 识别位点BsmAI识别位点BsrFI识别位点BsmBI识别位点BsrGI识别位点BsmFI识别位点BsrI 识别位点BsmI 识别位点BssHII识别位点BsoBI识别位点BssKI识别位点Bsp1286I识别位点BssSI识别位点BstAPI识别位点BstBI 识别位点BstEII 识别位点BstNI识别位点BstUI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI 识别位点BtgZI识别位点BtsCI 识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI 识别位点DdeI识别位点DpnI识别位点DpnII 识别位点DraI识别位点DraIII 识别位点DrdI识别位点。
常见分子实验限制性内切酶酶切位点大全
AatII识别位点Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI 识别位点BanI识别位点BanII识别位点BbsI识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsgI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstUI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点 ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I 识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IVHpyCH4V识别位点 KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI 识别位点MluI 识别位点MlyI 识别位点MmeI 识别位点MnlI 识别位点MscI 识别位点MseI 识别位点MslI 识别位点MspA1I 识别位点MspI 识别位点MwoI 识别位点NaeI 识别位点NarI 识别位点NciI 识别位点NcoI 识别位点NdeI 识别位点NgoMIV 识别位点NheI 识别位点NlaIII 识别位点NlaIV 识别位点NmeAIII 识别位点NotI 识别位点NruI 识别位点NsiI 识别位点NspI 识别位点PacI 识别位点PaeR7I 识别位点PciI 识别位点PflFI 识别位点PflMI 识别位点PhoI 识别位点PleI 识别位点PmeI 识别位点PmlI 识别位点PpuMI 识别位点PshAI 识别位点PsiI 识别位点PspGI 识别位点PspOMI 识别位点PspXI 识别位点PstI 识别位点PvuI 识别位点PvuII 识别位点RsaI 识别位点RsrII 识别位点SacI 识别位点SacII 识别位点SalI 识别位点SapI 识别位点Sau3AI 识别位点Sau96I 识别位点SbfI 识别位点ScaI 识别位点ScrFI 识别位点SexAI 识别位点SfaNI 识别位点SfcI 识别位点SfiI 识别位点SfoI 识别位点SgrAI 识别位点SmaI 识别位点SmlI 识别位点SnaBI 识别位点SpeI 识别位点SphI 识别位点SspI 识别位点StuI 识别位点StyD4I 识别位点StyI 识别位点SwaI 识别位点TaqαI 识别位点TfiI 识别位点TliI 识别位点TseI 识别位点Tsp45I 识别位点Tsp509I 识别位点TspMI 识别位点TspRI 识别位点Tth111I 识别位点XbaI 识别位点XcmI 识别位点XhoI 识别位点XmaI 识别位点XmnI 识别位点ZraI 识别位点与大家共享（非原创）。
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Acc65I 识别位点
AccI 识别位点
AciI 识别位点
AclI 识别位点
AcuI 识别位点
AfeI 识别位点
AflII 识别位点
AflIII 识别位点
AgeI 识别位点
AhdI 识别位点
AleI 识别位点
AluI 识别位点
AlwI 识别位点
AlwNI 识别位点
ApaI 识别位点
ApaLI 识别位点
ApeKI 识别位点
ApoI 识别位点
AscI 识别位点
AseI 识别位点
AsiSI 识别位点
AvaI 识别位点
AvaII 识别位点
AvrII 识别位点
BaeI 识别位点
BamHI 识别位点
BanI 识别位点
BanII 识别位点
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BbvCI 识别位点
BbvI 识别位点
BccI 识别位点
BceAI 识别位点
BcgI 识别位点
BciVI 识别位点
BclI 识别位点
BfaI 识别位点
BfuAI 识别位点
BglI 识别位点
BglII 识别位点
BlpI 识别位点
Bme1580I 识别位点
BmgBI 识别位点
BmrI 识别位点
BmtI 识别位点
BpmI 识别位点
Bpu10I 识别位点
BpuEI 识别位点
BsaAI 识别位点
BsaBI 识别位点
BsaHI 识别位点
BsaI 识别位点
BsaJI 识别位点
BsaWI 识别位点
BsaXI 识别位点
BseRI 识别位点
BseYI 识别位点
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BsiEI 识别位点
BsiHKAI 识别位点
BsiWI 识别位点
BslI 识别位点
BsmAI 识别位点
BsmBI 识别位点
BsmFI 识别位点
BsmI 识别位点
BsoBI 识别位点
Bsp1286I 识别位点
BspCNI 识别位点
BspDI 识别位点
BspEI 识别位点
BspHI 识别位点
BspMI 识别位点
BspQI 识别位点
BsrBI 识别位点
BsrDI 识别位点
BsrFI 识别位点
BsrGI 识别位点
BsrI 识别位点
BssHII 识别位点
BssKI 识别位点
BssSI 识别位点
BstAPI 识别位点
BstBI 识别位点
BstEII 识别位点
BstNI 识别位点
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BstXI 识别位点
BstYI 识别位点
BstZ17I 识别位点
Bsu36I 识别位点
BtgI 识别位点
BtgZI 识别位点
BtsCI 识别位点
BtsI 识别位点
Cac8I 识别位点
ClaI 识别位点
CspCI 识别位点
CviAII 识别位点
CviKI-1识别位点
CviQI 识别位点
DdeI 识别位点
DpnI 识别位点
DpnII 识别位点
DraI 识别位点
DraIII 识别位点
DrdI 识别位点
EaeI 识别位点
EaeI 识别位点
EagI 识别位点
EarI 识别位点
EciI 识别位点
EcoNI 识别位点
EcoO109I 识别位点
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EcoP15I 识别位点
EcoRI 识别位点
EcoRV 识别位点
FatI 识别位点
FauI 识别位点
Fnu4HI 识别位点
FokI 识别位点
FseI 识别位点
FspI 识别位点
HaeII 识别位点
HaeIII
HgaI 识别位点
HhaI 识别位点
HincII 识别位点
HindIII 识别位点
HinfI 识别位点
HinP1I 识别位点
HpaI 识别位点
HpaII 识别位点
HphI 识别位点
Hpy188I 识别位点
Hpy188III 识别位点
Hpy99I 识别位点
HpyAV 识别位点
HpyCH4III 识别位点
HpyCH4IV
HpyCH4V 识别位点
KasI 识别位点
KpnI 识别位点
MboI 识别位点
MboII 识别位点
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MfeI 识别位点
MluI 识别位点
MlyI 识别位点
MmeI 识别位点
MnlI 识别位点
MscI 识别位点
MseI 识别位点
MslI 识别位点
MspA1I 识别位点
MspI 识别位点
MwoI 识别位点
NaeI 识别位点
NarI 识别位点
NciI 识别位点
NdeI 识别位点
NgoMIV 识别位点
NheI 识别位点
NlaIII 识别位点
NlaIV 识别位点
NmeAIII 识别位点
NotI 识别位点
NruI 识别位点
NsiI 识别位点
NspI 识别位点
PacI 识别位点
PaeR7I 识别位点
PciI 识别位点
PflFI 识别位点
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PflMI 识别位点
PhoI 识别位点
PleI 识别位点
PmeI 识别位点
PmlI 识别位点
PpuMI 识别位点
PshAI 识别位点
PsiI 识别位点
PspGI 识别位点
PspOMI 识别位点
PspXI 识别位点
PstI 识别位点
PvuI 识别位点
PvuII 识别位点
RsrII 识别位点
SacI 识别位点
SacII 识别位点
SalI 识别位点
SapI 识别位点
Sau3AI 识别位点
Sau96I 识别位点
SbfI 识别位点
ScaI 识别位点
ScrFI 识别位点
SexAI 识别位点
SfaNI 识别位点
SfcI 识别位点
SfiI 识别位点
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SfoI 识别位点
TseI 识别位点
SgrAI 识别位点
Tsp45I 识别位点
SmaI 识别位点
Tsp509I 识别位点
SmlI 识别位点
TspMI 识别位点
SnaBI 识别位点
TspRI 识别位点
SpeI 识别位点
Tth111I 识别位点
SphI 识别位点
XbaI 识别位点
SspI 识别位点
XcmI 识别位点
StuI 识别位点
XhoI 识别位点
StyD4I 识别位点
XmaI 识别位点
StyI 识别位点
XmnI 识别位点
SwaI 识别位点
ZraI 识别位点
Taq αI 识别位点
TfiI 识别位点。