大豆品种资源对疫霉根腐病抗病性评价毕业论文

黑龙江大学

本科生毕业论文

论文题目：大豆品种资源对疫霉根腐病抗病性评价

学院：农业资源与环境学院

年级：2011级

专业：植物保护

姓名：郭亮

学号：

指导教师：马淑梅

2014年5 月7日

摘要

通过对大豆品种抗疫霉根腐病鉴定，旨在为抗病育种提供优良抗源和挖掘新的抗病基因。采用苗期下胚轴接种方法，对黑龙江和吉林大豆品种接种疫霉根腐病菌优势生理小种。结果表明：黑龙江省的137份大豆品种对1号生理小种表现抗病的有54个，占%，表现感病的有83个，占%。吉林省的54份大豆品种对1号生理小种表现抗病的有34个，占63%，表现感病的有20个，占37%。

大豆品种对多个生理小种（5个）的抗性鉴定结果表明，参鉴的黑龙江省99个大豆品种中，有41个品种抗1个或1个以上的小种，占鉴定总数的%。其中，有9个品种对5个小种表现为抗病，占%；有3个品种对4个小种表现为抗病，占%；有5个品种对3个小种表现为抗病，占%；有14个品种对2个小种表现为抗病，占%；有10个品种对1个小种表现为抗病，占%。

关键词

大豆品种；疫霉根腐病；抗性评价

Abstract

We identify soybean cultivar resistant to Phytophthora sojae to supply good anti-disease resource for breeding for disease resistance and discover new disease-resistant genes. We use seedling stage hypocotyl inoculation method to vaccinate advantage physiological seeds of Phytophthora sojae in Heilongjiang and Jilin soybean cultivar. Our results showed that there were 54 soybean cultivars in 137 cultivars in Heilongjiang Province resistant to Number 1 physiological seed, occupied %, and 83 cultivars were susceptible to disease, occupied %. While in Jilin Province, 34 cultivars in 54 soybean cultivars were resistant to Number 1 physiological seed, occupied 63%, 20 ones were susceptible, occupied 37%.

The identification results of soybean cultivar resistant to 5 physiological seeds revealed that in 99 soybean cultivars from Heilongjiang identified, there were 41 cultivars were resistant to one or more than one seed, occupied % of the total identified, and 5 cultivars resisted to 3 seeds, occupied %, as well as 14 cultivars were resistant to 2 seeds, occupied %, and 10 cultivars was able to resist to 1 seed, occupied %.

Key words

soybean cultivar; Phytophthora sojae; resistant evaluation

目录

摘要 ........................................................................................................................................ I 关键词 ........................................................................................................................................ I Abstract .................................................................................................................................... II 第一章前言 .. (1)

大豆疫霉根腐病概述 (1)

1.1.1大豆根腐病的分布和危害 (1)

1.1.2大豆根腐病菌的生物学特性 (1)

1.1.3大豆根腐病菌的侵染和流行规律 (2)

大豆疫霉根腐病致病性变异与研究目的 (2)

1.2.1大豆根腐病致病性变异 (2)

1.2.2研究目的 (3)

第二章试验材料与方法 (4)

供试材料 (4)

试验方法 (4)

2.2.1大豆植株培植 (4)

2.2.2鉴定方法 (4)

调查评价标准 (4)

第三章结果分析 (5)

大豆品种对疫霉根腐病的抗病性鉴定 (5)

3.1.1大豆品种对1号优势生理小种的抗性鉴定与评价 (5)

3.1.2大豆品种对多个生理小种的抗性鉴定与评价 (9)

3.1.3黑龙江省、吉林省以及国外的大豆品种抗性比较 (14)

第四章结论 (15)

参考文献 (17)

致谢 (19)