RT-PCR常用的内参引物

Human actin beta F305ctgggacgacatggagaaaa305-32452.3

BC002409R868aaggaaggctggaagagtgc868-84952.659.4564 F1379agcgagcatcccccaaagtt1379-139857.3

R1663gggcacgaaggctcatcatt1663-164456.354285 Rat actin beta F18cacccgcgagtacaaccttc18-3754.5

NM_031144R224cccatacccaccatcacacc224-20554.460.4207 F694gagagggaaatcgtgcgtgac694-71454

R1146catctgctggaaggtggaca1146-112753.257.1452 Mouse actin beta F91atatcgctgcgctggtcgtc91-11057.5

NM_007393R607aggatggcgtgagggagagc607-58857.860.4517 F1566gtccctcaccctcccaaaag1566-158554.5

F1831gctgcctcaacacctcaaccc1831-181154.455.7266 human GAPDH F369agaaggctggggctcatttg369-38855.6

BC004109R626aggggccatccacagtcttc626-60755.157.5258 F66aggtcggagtcaacggatttg66-8652.5

R597gtgatggcatggactgtggt597-57852.657532 Rat GAPDH F50cagtgccagcctcgtctcat50-6955

BC059110R644aggggccatccacagtcttc644-62555.158.3595 F1029acagcaacagggtggtggac1029-104854.4

R1280tttgagggtgcagcgaactt1280-126154.857.7252 Mouse GAPDH F306aggccggtgctgagtatgtc306-32554

M32599R835tgcctgcttcaccaccttct835-81654.658.7530 F49ggtgaaggtcggtgtgaacg49-6855.1

R281ctcgctcctggaagatggtg281-26254.556.8233 Rat 18S rRNA F1865tgcggaaggatcattaacgga1865-188658.6

M11188R2164agtaggagaggagcgagcgacc2164-21435959.3300 human 18S rRNA F1565cagccacccgagattgagca1565-158458.3

M10098R1816tagtagcgacgggcggtgtg1816-179758.359253 mouse 18S rRNA F56aggggagagcgggtaagaga56-7554.4

K013364R296ggacaggactaggcggaaca296-2775462241

大鼠432bp A001rat actin f TCAGGTCATCACTATCGGCAAT A002rat actin r AAAGAAAGGGTGTAAAACGCA 大鼠150bp A003rat actin f CCCATCTATGAGGGTTACGC

A004rat actin r TTTAATGTCACGCACGATTTC 人205bp A005h actin f TGACGTGGACATCCGCAAAG

A006h actin r CTGGAAGGTGGACAGCGAGG 人434 bp A007h actin f CGGGAAATCGTGCGTGAC

A008h actin r TGGAAGGTGGACAGCGAGG 小鼠263 bp A009m actin f GAGACCTTCAACACCCCAGC

A010m actin r ATGTCACGCACGATTTCCC

小鼠446 bp A011m actin f GAGACCTTCAACACCCCAGC

A012m actin r CCACAGGATTCCATACCCAA

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