常见载体引物表

合集下载

载体通用引物序列

PCDNA3.0-R2
5'-GGC AAC TAG AAG GCA CAG TC-3'
PCDNA3.1F
5'-CTA GAG AAC CCA CTG CTT AC-3’
PCDNA3.1R
5'-TAG AAG GCA CAG TCG AGG-3’
pCEP Frorward
5'-AGC AGA GCT CGT TTA GTG AAC CG-3'
P31430
5'-CGT TTT AAAACCTAAGAGTCAC-3'
P12584
5'-TTT TCA GTA TCT ACG AT-3’
P17110
5'-TAC CAC TAC AAT GGA TG-3’
PAS2-1F
5'-TCA TCG GAA GCG AGT AG-3'
PAS2-1R
5'-CGT TTT AAA ACC TAA GAG TCA C-3'
PQE30R
5'-GTT CTG AGG TCA TTA CTG G-3'
pTARGET-SEQ
5'-TTA CGC CAA GTT ATT TAG GTG ACA-3’
PTRC99C-F
5'-TTG CGC CGA CAT CAT AAC-3’
PTRC99C-R
5'-CTG CGT TCT GAT TTA ATC TG-3’
PEGFP-C-3’
5'-TAT GGC TGA TTA TGA TCA GT-3'
PEGFP-C-5’
5'-CAT GGT CCT GCT GGA GTT CGT G-3'

载体及其上下游引物

载体名称上游引物（5'primer）下游引物（3'primer）P3*FLAG-CMV CMV-30(825-854)CMV-24(1129-1152)pAc5.1/V5-His(_A,_B,_C) pAc5-5 BGH revpAcG2T pGEX-5 not availablepACT T7EEV T3/EBVrevpACT2GAL4 ADfor pGAL4-ADrvpACT2p17110p12584pACYC184(BamHI-site) PBRforBam PBRrevBampACYCDuet-1(MCSI)ACYCDuetUP1 Primer DuetDOWN1 pACYCDuet-1(MCSII) DuetUp2 T7-TerminatorpAdEasy-1 not available not availablepAdTrack-CMV not available not availablepAS2-1GAL4 Bdfor/P24990pGAL4-BDrv/P31430pB42AD pB42ADF PB42ADRpBABE pBMN_5'　pBacPAK8BAC1BAC2pBAD pBAD-F pBAD-RpBAD/HisABC pBAD-5'　pBBR1MCS T3/M13rev T7/M13forpBD-GAL4pBD-GAL4-F PBD-GAL4-RpBI12135S NOSpBIND GAL4-Bdfor T3,EBVrevpBK-CMV M13F/T7M13R/T3 pBluescriptIIKS(+/-) T3/M13rev T7/M13for（优先用M13F）pBluescriptIISK(+/-) T3/M13rev T7/M13for pBluescriptKS(+/-) T3/M13rev T7/M13for pBluescriptSK(+/-) T3/M13rev T7/M13forpBR322(BamHI-site) PBRforBam PBRrevBampBR322(EcoRI/HindIII-sites) pBRforEco pBRrevHindpBR325(BamHI-site) PBRforBam PBRrevBampBR329(BamHI-site) PBRforBam PBRrevBampBridge(MCSI) GAL4-Bdfor3'BDpBridge(MCSII) not available not availablepBS-T II T3/M13rev T7/M13forpBS（AMP）T7T3pBSR T3T7terpBudCE4.1(MCSI) CMV-Profor,T7 c-mycrev,EBVrevpBudCE4.1(MCSII)EF-1α Fwd BGHpBudCE4.1/lacZ/CAT CMV-Profor,T7 c-mycrev,EBVrevpBV220pBV220F PBV220RpCAL-c T7/Tac-Profor T7-TerminatorpCAL-n T7/Tac-Profor T7-Terminator pCAMBIA1301(1300)pCAMBIA1301-5'(ECORI)/M13R pCAMBIA1301-3'(HindIII) pCAMBIA1304pCAMBIA1301-5'(ECORI)pCAMBIA1301-3'(HindIII) pCAMBIA2300M13R(-48)M13F(-47)/M13F(-49) pCANTB5E S1S6pCAT3-enhancer RVP3此载体无反向引物pCDFDuet-1(MCSI)ACYCDuetUP1 Primer DuetDOWN1 pCDFDuet-1(MCSII)DuetUP2T7TERpcDNA1.1 CMV-Profor,T7 pCDM8-3pcDNA2.1 M13for/T7 M13rev/Tac-Profor pcDNA3 T7/CMV-Profor BGH rev/SP6pcDNA3.1(+/-) pcDNA3.1F/T7/CMV-Profor BGH revpcDNA3.1/His(_A,_B,_C) T7/CMV-Profor BGH revpcDNA3.1/myc-His(_A,_B,_C) T7/CMV-Profor BGH rev/c-mycrev pcDNA3.1/V5-His(_A,_B,_C) T7/CMV-Profor BGH rev/V5revpcDNA3.1/Hygro(+,-) T7/CMV-Profor BGH rev/V5revpcDNA3.1/Zeo(+.-) T7BGHrevpcDNA3.3TOPOTA CMV-Profor TKPAreversepcDNA4/HisMax(_A,_B,_C) Xpressfor BGH revpcDNA4/HisMax-TOPO Xpressfor BGH revpcDNA4/HisMax-TOPO/lacZ Xpressfor BGH revpcDNA4/myc-His(_A,_B,_C)T7,CMV-Profor BGH rev,c-mycrev pcDNA4/TO TREfor/CMV-Profor BGH revpcDNA4/V5-His(_A,_B,_C) CMV-Profor,T7 BGH rev,V5revpcDNA4-TET/ON/AMP+CMV-Profor　pcDNA5/FRT T7/CMV-Profor BGH revpcDNA6/myc-His(_A,_B,_C) CMV-Profor,T7 BGH rev,c-mycrev pcDNA6/V5-His(_A,_B,_C) CMV-Profor,T7 BGH rev,V5revpCEP4pCEP-F/CMV-Profor EBVrevpCI T7（17BASE） EBVrevpCI-neo T7（17BASE） EBVrev,T3pCMS-EGFP T7 T3pCMS-EGFP T7 EBVrev,T3pCMV-3Tag-4A T3 /PFLAG-CMV-F T7pCMV5pCMV5F PCMV5RpCMV6 CMV-Profor pCMV6-3、HGHrev pCMV6-XL4 CMV-Profor/T7 pCMV6-3、HGHrev pCMV-HA PCMV-F,CMV-Profor,TREfor PCMV-R，EBVrev pCMV-MYC(AMP+)PCMV-F PCMV-R, EBVrev pCMVmyc/nuc CMV-Profor BGH rev,c-mycrevpcmv-scriptEXVector T3 T7pCMV-Sport6 T7/M13for SP6/M13rev pCOLADuet-1(MCSI)ACYCDuetUP1 Primer DuetDOWN1pCOLADuet-1(MCSII)DuetUP2T7TERpCR2.1 T7,M13for M13revpCR2.1-TOPO M13for,T7 M13revpCR3.1 T7 BGH revpCR3.1-Uni T7 BGH revpCR4 T3/M13rev T7/M13forpCR4-Blunt T3/M13rev T7/M13forpCR4-TOPO M13rev,T3 M13for,T7pCR-Blunt M13rev T7,M13forpCR-BluntII M13rev/SP6 T7/M13forpCR-BluntII-TOPO M13rev/SP6 M13for/T7pCRII M13rev/SP6 T7/M13forpCRII-TOPO M13rev/SP6 M13for/T7pCR-ScriptSK(+) T7/M13for T3/M13revpCR-XL-TOPO M13rev T7,M13forpCS2+(MCSI) SP6 EBVrev,T7?pCS2+(MCSII) T3 pSG5-3pDB_GAL4cam T7P　pDB_leu PDBLeu-5 PDBLeu-3pDEST22DEST22F DEST22RpDEST15 pGEX-5 T7-TerminatorpDEST20 pGEX-5 pFastBac-3pDEST26 TREfor/CMV-Profor T7/M13forpDisplay T7/CMV-Profor c-mycrevpDNR-LIB(MCS_A) M13for,T7 pSG5-3,M13RpDonar M13F M13RpDONR201PDONR-F PDONR-RpDONR207PDONR-F PDONR-RpDONR223M13F M13RpDrive T7/M13rev SP6/M13forpDsRED1-C1(DsRed1-N-f)DsRed1-C-rpDsRed1-N1 CMV-Profor RFP-Nrev,CMV-R? pDsRed2-N1(DsRed1-N-f)DsRed1-C-rpDsRed2-C1(DsRed1-N-f)DsRed1-C-rpDsRED2-C1(KAN+)pDsRED-ex-C1-F pEGFP-N-3’pDsRed-Express dsRed1_N_primer/CMV-F dsRed1_C_primer/CMV-R pDSRED-monomer-N1(KAN+)dsRed1_N_primer　pDSRED-N1(KAN+)pEGFP-N-5’ pDSRED-N-RpEASY-Blunt M13F T7/ M13RpEASY-BluntSimple M13F T7/ M13R（距离插入位点合适，优先安排）pEASY-T1 Simple M13F M13R（距离插入位点合适，优先安排）pEASY-T1M13F/T7M13RpEASY-T3M13F/T7M13R/SP6pECFP-N(C)PECFP-N-5.PECFP-N-3,pEF/myc/cyto/ER/mito/nuc pEF-5Pcdna3.1R/BGH revpEF1(4,6/myc-His) T7/pEF-5Pcdna3.1R/BGH revpEF1(4,6/V5-His) T7/pEF-5Pcdna3.1R/BGH revpEF1(4;6/His_A;_B;_C) T7/Xpressfor Pcdna3.1R/BGH revpEF5/FRT/V5-D(-TOPO) T7/pEF-5Pcdna3.1R/BGH rev pEGFP M13rev EGFP-NrevpEGFP-1 not available EGFP-Nrev/ PEGFP-N-3′pEGFP-C PEGFP-C-5′PEGFP-C-3′pEGFP-C1 EGFP-Cfor SV40-pArevpEGFP-C2 EGFP-Cfor SV40-pArevpEGFP-C3 EGFP-Cfor SV40-pArevpEGFP-F EGFP-Cfor SV40-pArevpEGFP-N PEGFP-N-5′PEGFP-N-3′pEGFP-N1 CMV-Profor EGFP-NrevpEGFP-N2 CMV-Profor EGFP-NrevpEGFP-N3 CMV-Profor EGFP-NrevpENTR M13F M13RpET-11(-a,-b,-c,-d) T7 T7-TerminatorpET-12(-a,-b,-c) T7 T7-TerminatorpET-14b T7 T7-TerminatorpET-15b T7 T7-TerminatorpET-16b T7 T7-TerminatorpET-17b T7 T7-TerminatorpET-17xb T7 T7-TerminatorpET-19b T7 T7-TerminatorpET-20b(+) T7 T7-TerminatorpET-21(-a,-b,-c,-d)(+) T7 T7-TerminatorpET-22b(+) T7 T7-TerminatorpET-23(-a,-b,-c,-d)(+) T7 T7-TerminatorpET-24(-a,-b,-c,-d)(+) T7 T7-TerminatorpET-25b(+) T7 T7-TerminatorpET-26b(+) T7 T7-TerminatorpET-27b(+) T7 T7-TerminatorpET-28a(-b,-c)(+) T7 T7-TerminatorpET-29a(-b,-c)(+)S.tag/T7 T7-TerminatorpET-3(-a,-b,-c,-d) T7 T7-TerminatorpET-30_Ek/LIC T7 T7-TerminatorpET-30_Xa/LIC T7 T7-TerminatorpET-30a(-b,-c)(+)S.tag/T7 T7-TerminatorpET-31b(+) not available T7-TerminatorpET-32_Ek/LIC TRXfor T7-Terminator pET-32_XA/LIC TRXfor T7-Terminator pET-32a(-b,-c)(+)S.tag/TRXfor T7-Terminator pET-33b(+) T7 T7-Terminator pET-34b(+)S.tag T7-Terminator pET-35b(+)S.tag T7-Terminator pET-36b(+)S.tag T7-Terminator pET-37b(+)S.tag T7-Terminator pET-38b(+) not available T7-Terminator pET-39b(+)S.tag T7-Terminator pET-40b(+)S.tag T7-Terminator pET-41_Ek/LIC GST-Cfor T7-Terminator pET-41a(-b,-c)(+) S.tag/pGEX-5 T7-Terminator pET-42a(-b,-c)(+)S.tag/pGEX-5 T7-Terminator pET-43.1a(-b,-c)(+)S.tag coliDOWNpET-43.1a_EK/LIC S.tag not availablepET-44a(-b,-c)(+)S.tag T7-Terminator pET-44a_EK/LIC S.tag not availablepET-45b(+) T7 T7-Terminator pET-46_Ek/LIC T7 T7-Terminator pET-5(-a,-b,-c) T7 T7-Terminator pET-52b(+) T7 T7-Terminator pET-7 T7 T7-Terminator pET-9(-a,-b,-c,-d) T7 T7-Terminator pETcoco-1 T7 T7-Terminator pETDuet-1(MCSI)pETUP1DuetDOWN1 pETDuet-1(MCSII)DuetUp2T7-Terminator pET-T4 T7 T7-Terminator pEYFP-C1pEGFP-C-5’ pEGFP-C-3' pEYFP-N1pEGFP-N-5’,CMV-Profor pEGFP-N-3’ pFLAG-ATS N26for pTrcHis-rv；C24rev pFLAG-CTC N26for pTrcHis-rv；C24rev pFLAG1N26for pTrcHis-rv；C24rev pFLAG-CMV(-2)CMV-30；CMV-Profor CMV-24pFLAG-CMV1CMV-30CMV-24pGAD424GAL4_ADfor3'ADpGADGH GAL4_ADfor3'ADpGADT7T7/GAL4_ADfor3'ADpGADT7-Rec T7/GAL4_ADfor3'ADpGADT-T T73,BDpGAD10GAL4_ADfor3'ADpGAPZa-A a-FACTOR3'AOXpGBKT7T73,BDpGBT9MATCHMAKER5'DNA-BD/GAL4BD MATCHMAKER3'DNA-BDpGEM-1 T7 SP6pGEM-3 T7 SP6pGEM-3Zf(+)(-) SP6/M13rev T7/M13for pGEM-4 T7 SP6pGEM-4Z T7/M13for SP6/M13rev pGEM-5Zf(+)(-) SP6/M13rev T7/M13for pGEM-7Zf(+)(-) SP6/M13rev T7/M13for pGEM-9Zf(+)(-) T7/M13for SP6/M13rev pGEM-T T7/M13for SP6/M13rev pGEM-T_Easy T7/M13for SP6/M13rev pGene/V5-His_A(_B,_C) pGENE-5 BGH rev,V5rev pGEX-2T pGEX-5 pGEX-3 pGEX-2TK pGEX-5 pGEX-3 pGEX-3X pGEX-5 pGEX-3 pGEX-4T-1(-2,-3) pGEX-5 pGEX-3 pGEX-5X-1(-2,-3) pGEX-5 pGEX-3 pGEX-6P-1(-2,-3) pGEX-5 pGEX-3pGL2GLP1 GLP2pGL3RVP3GLP2(RVP4)(没有特别说明用GLP2)pGL3-Basic(MCSI) RVP3(PGL3+)? GLP2(PGL3-)?pGL3-Basic(MCSII) RVP4, PLXSN-3'?pGL4 pGlow(-TOPO) T7 pGlow-3pGM-T T7SP6pGWC SP6T7pHook-2 T7/CMV-Profor not availablepIND/V5-His_A(_B,_C) not available BGH rev,V5rev PinpointTMVector PinpointPrimer SP6pIRES(MCSI) T7 pIRES-3pIRES(MCSII) not available T3,EBVrevpIRES2-EGFP CMV-Profor,pIRES2-EGFP.P5’ IRESrev,pIRES2-EGFP.P3’pIRES-hrGFP-2a CMV-Profor,T3 pIRES-hrGFP-3 pIRESneo2 T7/CMV-Profor pIRES-3pIVEX(all) T7/pBRrevBam T7-TerminatorpLEXA pLEXA-F pLEXA-RpLNCX pLNCX-F pLNCX-RpLNCX2CMV-Profor pRevTRE-3pLVTHM（H1启动子下游）H1primer SP6pLXSN pLXSN-F pLXSN-RpMALc MalE primer M13F(-47)pMAL-c2E MalE primer M13F(-47)pMAL-C2x Pmal-c2x-F(P5’)Pmal-C2X-R(P3’ ) pMALd MalE primer M13F(-47)pMALe MalE primer M13F(-47)pMAL-P4X MalE primer M13F(-47)pMAL-p2X MalE primer M13F(-47)pMET A/B/C AUG1F AUG1RpMD18-T M13R(-48)ECORI M13F(-47)(HINDⅢ) pMD19-T M13F47M13R48pMD20-T M13F47M13R48 pMOSBlue T7/M13rev M13forpMSCV-puro pLXSNfw pMSCVrevpMT/V5-His_A(_B,_C) Mtfor BGH rev,V5rev pNTAP T3T7pCTAP pCTAP5'primer T7pOTB7 M13rev/T7 M13for/SP6 pOptiVEC-TOPO TA CMV-F/CMV-Profor EMCVIRES reverse pPC86PPC86-F PPC86-RpPC97PBD-GAL4-F PBD-GAL4-RpPIC3.5K5'AOX3'AOXpPic9k(A+,Z+)5’AOX(Ppic9k-f)/a-factor3’AOX(Ppic9k-R) pPICZa5'AOX/PPICZa-F3'AOX pPROEXHTA M13R(-48)无反向引物pProEX-HTa(b,c)M13R48/ Tac-Profor pBADrv/pTrcHis-3 pPD129.36　M13F20/M13FpQE30or40or60(AMP+)PQE30-F PQE-30RpRC/CMV2/CAT CMV/T7　pRECEIVER CMV-F 此载体无反向引物pRECEIVER-M03pRECEIVER-M03F pRECEIVER-M03R pREP4pREP4 forward primer EBV reverse primer pRK5 SP6 pSG5-3pRSET T7/Xpressfor T7terpRSFDuet-1(MCSI)DuetUP1DuetDOWN1 pRSFDuet-1(MCSII)DuetUP2T7TERpSecTac2 T7/CMV-Profor BGH rev/c-mycrev pSG5 pSG5-5,/T7PSG5-R(SV40) pSHlox1 T7 SP6pSHUTTLE-CMV PSHUTTLE-CMV-F PSHUTTLE-CMV-R pSI T7 EBVrevpSilencer1.0-U6 T7 T3pSilencer2.0-U6 T7 M13forpSilencer2.1-U6neo T7 M13forpSilencer3.1-H1hygro M13F(-47) 3.0REVpSilencer4_1-CMVneo pSilener4.1-5' CMV-Profor pSILENCEV2.0-U6T7 2.0REVpSIneo T7 EBVrev,T3Psit T7 T7TpSK01-T M13F M13RpSK-CMV(KAN+)T7 T3pSOS Psos5'Psos3'pSP64 SP6 M13revpSP64/65SP6 此载体无反向引物pSP65 SP6 M13revpSP70 SP6 T7pSP71 SP6 T7pSP72 SP6 T7pSP73 SP6 T7pSport1SP6/M13F(-47)T7/M13R pSPORT1 SP6/M13for T7/M13revpSPT18 SP6 T7pSTBlue T7/M13rev SP6/M13for pSUPER.Basic T7/M13F M13RpSUPER T7T3pSURE-T M13F M13RpSURE-Tsimple M13F-47M13R-48 pSuperCos pBRforEco T7pT3T7 M13rev/T3 M13for/T7pT7-7 T7 not availablepT7Blue®T7 M13F(-47)pT7T3_18U M13rev/T7 M13for/T3pT7T3D-PAC M13rev/T7 M13for/T3pTA2M13F/T7(优先使用M13F）T3/M13RpT-Adv M13rev T7,M13forpTAg T7/M13rev M13for pTARGETTM pTarget.F(在T7前面）/T7 pTarget.RpTG19-T M13F(-47)M13R(-48) pTARGETTM T7/M13F PTARGET-SEQ pThioHisA,B,andC Trx Forward Trx Reverse / M13for pTO-T7T7 T7TERpTRC99A PTRC99A-F pTrcHis-rvpTrc99a-c PTRC99C-F PTRC99C-RpTRC99a-c PTRC99C-F PTRC99C-R pTrcHisB PTrcHisA-F/Xpressfor PTrcHisA-R pTrcHisA PTrcHisA-F PTrcHisA-RpT-REX-DEST30 TREfor T7pTriEx1 T7/TriExup TriExdownpTriEx1.1 T7/TriExup TriExdownpTriEx1.1_Hygro T7/TriExup not availablepTriEx1.1_Neo T7/TriExup IRESrevpTriplEX1 TriplEx-LD-5 T7,TriplEx-LD-3pTriplEX2 TriplEx-LD-5；pTR5‘ T7,TriplEx-LD-3pTWIN-1T7 T7TERpTXB1 T7 MxeInteinrevpTXB2 T7 MxeInteinrevpTXB3 T7 MxeInteinrevpTYB1 T7 InteinrevpTYB2 T7 InteinrevpTYB3 T7 InteinrevpTYB4 T7 InteinrevpTZ_18U T7/M13rev M13forpUB6/V5-His UB-F BGH rev,V5revpUC13 M13rev/M13rev(-48) M13for/M13for(-49)pUC18 M13rev/M13rev(-48) M13for/M13for(-49)pUC18(19)/118(119)M13F(-47)M13R(-48)/PUC19(HINDⅢ) pUC19 M13rev/M13rev(-48) M13for/M13for(-49)pUC57 M13rev/M13rev(-48) M13for/M13for(-49)pUC8 M13rev/M13rev(-48) M13for/M13for(-49)pUC9 M13rev/M13rev(-48) M13for/M13for(-49)pUM-T M13F(-20)M13R(-20)pUCm-T(AMP+)M13F/T7M13RpUNI/V5-His not available BGH rev,V5revpVAX1T7 T7/CMV-Profor PCDNA3.1R/BGHRpVP22/mycHis VP22-Cfor BGH rev,c-mycrevpVP22/mycHis2 T7/CMV-Profor VP22-NrevpWE15 pBRrevHind T7pYES2 T7pYes2.RpYES-DEST52 T7/GAL1-Profor V5rev/CYC1-Terminator pYEX_4T_1 Clontech pYEX_4T-3pYEX_4T_2 pGEX-5 pYEX_4T-4pYEX_4T_3 pGEX-5 pYEX_4T-4pYEX_4T_4 pGEX-5 pYEX_4T-4pZeoSV2+/hu-Tyr T7 not availablepZERO-1 M13rev/SP6 T7/M13forpZERO-2 M13rev/SP6 M13for/T7pZome-1-C Zome-F Zome-RpZome-1-N Zome-F Zome-RYCp50(BamHI-site) PBRforBam PBRrevBamYEp13(BamHI-site) PBRforBam PBRrevBamYEp24(BamHI-site) PBRforBam PBRrevBamYIp5(BamHI-site) PBRforBam PBRrevBam RNAi-ReadypSIREN-RetroQ-U6ZsGreen U6　菌PCDB T7BGH天为时代TA载体T7T3m13（+）T7？T3？pMSCV-neo pMSCV-5'pMSCV-3'。

通用引物列表

引物名称 M13R M13F
序列(5'-3') CAG GAA ACA GCT ATG ACC TGT AAA ACG ACG GCC AGT
M13F(-47) CGC CAG GGT TTT CCC AGT CAC GAC M13R(-48) AGC GGA TAA CAA TTT CAC ACA GGA M13(-96) SP6 CCC TCA TAG TTA GCG TAA CG ATT TAG GTG ACA CTA TAG
TAT GGC TGA TTA TGA TCA GT AAG AAG GGC AGC ATT CAA AG ATG GAC TAT CAT ATG CTT ACC GTA AGG CGA TTA AGT TGG GTA GAG TTA GCT CAC TCA TTA GGC GAA CGC CAG CAC ATG GAC CTC CGA GAT CTG GAC GAG C GAC GAT AGT CAT GCC CCG CG CTA GCA AAA TAG GCT GTC CC GTT CTG AGG TCA TTA CTG G TGA GCG GAT AAC AAT TTC AC TCA AGC CTC AGA CAG TGG TTC TGA AGC GCA TGA ACT CCT TG
T7 TAA TAC GAC TCA CTA TAG GG T7 terminator TGC TAG TTA TTG CTC AGC GG T3 pGEX-4T5' pGEX-4T3' GLp1 GLp2 RVp3 RVp4 pcDNA3.1 R PinPoint primer pCMV-F ATT AAC CCT CAC TAA AGG GA GGG CTG GCA AGC CAC GTT TGG TG CCG GGA GCT GCA TGT GTC AGA GG TGT ATC TTA TGG TAC TGT AAC TG CTT TAT GTT TTT GGC GTC TTC CA CTA GCA AAA TAG GCT GTC CC GAC GAT AGT CAT GCC CCG CG TAG AAG GCA CAG TCG AGG CGT GAC GCG GTG CAG GGC G TCT AAA AGC TGC GGA ATT GT

载体与引物表(完全)

M13R T3/M13R/M13R(-48)
SP6 PYES2.R
M13R T3
载体抗性 Amp+
Amp+ Amp+ Amp+ Amp+ Amp+ Amp+ Amp+ Amp+
Amp+ Amp+ Amp+ Amp+
共3第3页
PM
PM-F
pMAL-c2E
MalE primer
pMAL-C2x
P5’
pMAL-p2X
MalE primer
pMD18-T
PQE30F/M13R(-48)/RV-M
Pmep4
pMEP4-F
pPIC9K(A+,Z+)
5’AOX(pPIC9K-F)
PPICZa pPROEXHTA
5'AOX/PPICZa-F M13R(-48)
pcDNA3.1R BGH
Amp+
M13F
M13R
pCEP-F pCI-F2/ T7(17base)
EBV-R (此载体没有反向引物)EBR
pCI-F2/ T7(19/17base)
T3
Amp+
T7 2G
T3
pCMV-F
pCMV-R
T3 /PFLAG-CMV-F
T7
Pcmv5f
Pcmv5r
Amp+
Pcmv5f
PDC316.R Primer(R)
载体抗性
pDonar
M13F
M13R
pDONR207
FORWARD
REVERSE
Pdonr221
M13F

常见的通用引物序列

T7TER
5’TGCTAGTTATTGCTCAGCGG3'
S.TAG
5’CGAACGCCAGCACATGGACA3’
T3
5'ATTAACCCTCACTAAAGGGA3’
M13F（47）
5'CGCCAGGGTTTTCCCAGTCACGAC3’
M13R（48)
5'AGCGGATAACAATTTCACACAGGA3'
TGC GTA CTG CGG TGA TCA AC
P3'
CTG CAA GGC GAT TAA GTT GG
DuetDOWN1
5’-GATTATGCGGCCGTGTACAA-3'
PET Upstream
ATG CGT CCG GCG TAG AGG AT
DuetUP2
5'-TTGTACACGGCCGCATAATC-3’
PTRCHISAF
5’CAT GGT ATG GCT AGC ATG AC3’
PTRCHISAR
5'CAG GCT GAA AAT CTT CTC TC3'
pDC316 F
5’ —TAA CTT GTT TAT TGC AGC —3'
pDC316 A
5' -TAT GCT TAC CGT AAC TTG -3'
PEFF
5' TCA AGC CTC AGA CAG TGG TTC3’
PCEPF
5’ AGA GCT CGT TTA GTG AAC CG3’
EBVR
5’ GTG GTT TGT CCA AAC TCA TC3’
CMVF
5'CGCAAATGGGCGGTAGGCGTG3'

常见载体引物表

CMV-F
无
pDSRED-PRESS-C1
pDSRED -C1-F
pDSRED-C1-R
pCR-Blunt
M13R
M13F/T7
pMT/V5-His_A (_B,_C)
MT-Profor
BGHrev
pEASY-T3
T7/M13F
SP6/M13R
PSILENCE-1.0-U6
T7
2.0REV(同P2(pCAMBIA 1301))
pMSCV
MSCV5'
MSCV-rev
pBABE
pBABE5’
pBABE3’
pFastBac HT_A (_B, _C)
pFastBac-F
pFastBac-R
pFastBac1
PH-Profor/BaculoVfor
pFastBac-R
pACYCDuet-1 (MCS I)
ACYCDuetUP 1
T7/S.tag
T7 Ter
pET-42a (-b, -c)(+)
Stag
T7 Ter
pET50(amp+)
T7/s.tag
T7TER
pET44(AMP+)
T7/s.tag
T7 Ter
pEYFP-N1
pEGFP-N-5’
pEGFP-N-3’
PETBlue
PETBlueT7UP(太靠前，在T7前)/ PETBlueUP
pLEXA-R
pCDH
pCDH-EF1
无
PVX
LBA
LBB
pAcGP67 (_A, _B, _C)
PH
pAcGP67rv
pAD-Track

常用载体测序引物列表

常用载体测序引物列表载体名称正向引物反向引物pACT T7 T3pACT2 GAL4 AD(5’AD) 3'AD/pACT2-R PACYCDUET-1(MCSII) DuetUP2 T7TERpAD/pl-DEST CMV-FpAD-Track pAD-Track-F 无pAS2-1 5’BD M13-26/48 PB42AD PB42ADF PB42ADR PBAD PBAD-F PBAD-R pBABE pBABE5’ pBABE3’ PBACPAK8 BAC1BAC2 pBK-CMV T7/M13-20 T3/M13-26 PBS(SK/KS)/M13- M13F/T7T3/M13R pBV220 PBV220F PBV220R pBI121需根据酶切位点确认需根据酶切位点确认 pCAMBIA 1301(1300)需根据酶切位点确认需根据酶切位点确认 pCAMBIA 2300 需根据酶切位点确认需根据酶切位点确认PCANTB5E S1/M13R S6pCAT3-enhancer pGL3+pcDNA3.0 pEGFP-N5/T7 SP6/BGHpcDNA3.1 pEGFP-N5/T7 BGHpcDNA4 T7/pEGFP-N5 BGHpcDNA6 T7/pEGFP-N5 BGHpcDNAII T7SP6 pCE2.1 M13FM13R pCEP4 pCMV5R/PCEP-F pEGFP-N5/EBV-R pCF-T M13FM13R pCIT7（17Base ） pCMV5R pCI-neo T7（17Base ） T3pCMS-EGFP T7T3 pCMV-3Tag-4A T3 /pFlag-CMV-F T7pCMV5 pCMV5F pCMV5R pCMV5-Flag pCMV5FpCMV5R pCMV-MYC/NUC pCMV5FpCMV5R PCMV-HA pCMV5FpCMV5R pCMV-SportSP6/M13RpCMV-Tag T3T7 pCMV-TNT T7/SP6pCMV5R pCR2.1-TOPO M13F/T7M13R pCR3.1 T7BGH pCS2 SP6T7PDNR-LIB M13F M13RpDonar M13F M13R pDONR201 pDONR-F pDONR-R pDONR221 M13F M13RpDrive T7 SP6 pDRIveR T7 SP6pDsRED1-C1 pDsRED-C1-F pECFP-C-3 pDsRed1-N1 pEGFP-N-5 RFP-NrevpDsRed-Monomer-N1 pEGFP-N-5 pDsRed1-NpDsRED2-C1 pDsRED-ex-C1-F pEGFP-N-3’pEGFP-N-5 pDsRed1-N pDSRED2-N1pDSRED-N1 pEGFP-N-5’ PDSRED-N-R pEASY-BLUNT M13F/T7 M13RTerpEASY-E1 T7 T7pEASY-T1 M13F/T7 M13RpEASY-T3 M13F/T7 M13R/SP6 pECFP-C pEGFP-C-5’ pECFP-C-3' pECFP-N1 pEGFP-N-5’ pEGFP-N-3’pEF/myc/ER pEF-F pCDNA3.1R pEGFP-C pEGFP-C-5’ pEGFP-C-3' pEGFP-N pEGFP-N-5’ pEGFP-N-3’ pENTR/D-TOPO M13F M13RTerpET-*(His) T7 T7pET22(a,b,c) T7 T7TerTerpET28,30,24,49 T7 T7TerpET32(a,b,c) T7/S.tag T7pET-42a (-b, -c)(+) Stag T7 TerpET50 T7/s.tag T7TERpET44 T7/s.tag T7Ter pEYFP-N1 pEGFP-N-5’ pEGFP-N-3’ PETBlue M13-20 PETBlueDOWN PETDUET-1(MCSI) PBRrevBam DuetDOMN1PETDUET-1(MCSII) DuetUP2 T7TERpEYFP-C1 pEGFP-C-5’ pEGFP-C-3' pEYFP-N1 pEGFP-N-5’ pEGFP-N-3’ pFastBac pFastBac-F pFastBac-R pFastBac HT_A,B,C pFastBac-PH pECFP-C-3 pFastBac1 pFastBac-PH pECFP-C-3 pFastBac Dual (MCSI) pFastBac-PH pECFP-C-3 pFLAG-CMV CMV30/pFLAG-CMV-F CMV-24/pFLAG-CMV-Rp3xFlag-CMV-7.1 CMV-F/CMV24 CMV30pGAD424 5’AD 3'ADpGADGH 5’AD 3'ADpGADT7-Rec 5’AD/T7 3'ADpGAPZa-A/B/C a-FACTOR 3'AOXforward 3'AOXpGAPZ-A/B/C pGAPpGBKT7 T7 3'BDpGEM-T(-Easy) M13F/T7 SP6/M13RpGEX-(*)T pGEX-5’ pGEX-3’pGL2 pCMV5R PGL3-Pgl3-BASIC pGL3+ PGL3-pGL3-Enhancer pGL3+ PGL3-PinPoint TM PinPoint Primer SP6pIRES PIRES-F/T7 T3/PIRES-RpIRES2-EGFP pIRES2-EGFP.P5’ pIRES2-EGFP.P3’ pLenti6/v5-Dest pEGFP-N-5’PLEXA PLEXA-F PLEXA-R PLNCX PLNCX-F PLNCX-R pLXSN pLXSN-F pLXSN-RPjc1-tac T7pJet.1.21.Blunt T7primer M13F(-47) pMAL-c2E MalEP3/PMAL-C2X-R pMAL-C2x P5/PMAL-C2X-Fprimer M13F(-47) pMAL-p2X MalEPMD18-T M13R(-48) M13F(-47) PMD19-T M13F(-47) M13R(-48) pMIR-report M13FPPC86 PPC86-F PPC86-R3’AOXpPIC9K 5’AOX/a-factor3'AOX pPICZa 5'AOX/PPICZa-FpPROEXHTA M13R(-48)pQE30or40 pQE30+ pQE30- pRECEIVER CMV-FpRSET T7 T7TER PRSFDUET-1(MCSI) DuetUP1 DuetDOWN1 PRSFDUET-1(MCSII) DuetUP2 T7TERpShuttle-CMV pShuttle-CMV-F pShuttle-CMV-R PSILENCE-1.0-U6 T7 T3pSilencer3.1-H1 hygro M13F(-47) 3.0revpSILENCEV2.0-U6 T7 2.0revpSK01-T M13F M13RpSK-CMV T7 T3Psos H1PSP64/65 SP6pSP72 T7 SP6T7/M13RpSport1 SP6/M13F(-47)pSTBlue-1 T7 SP6pSUPER T7 T3pT7Blue(R) T7 M13F(-47)pTA2 M13F/T7 T3/M13R/T7 M13RpT-Adv M13FpTARGET TM pTarget-F/T7 arget-RPTHIOHISA,B,C TRX-FORWARD TRX-REVERSEpTO-T7 T7 T7TERPBV220-/PTRC99C-R pTRC99a-c PQE30+/PTRC99C-FpTriPLEx2 PT5/5'TriplEx2 T7pTz57r/t M13+ M13-pTWIN-1 T7 T7TERpUC18(19)/118(119) M13F(-47) M13R(-48)M13F(-47)/M13F pUC57 M13R(-48)/M13RpUCm-T M13F/T7 M13RpVAX1 T7 BGHT3/M13R/M13R(-48) pWSK29 T7/M13F/M13F(-47)pXT7 T7 SP6pYES2 T7 pYes2.R。

表4 主要使用的引物及序列

表4 主要使用的引物及序列序列技术在现代生物学研究中发挥着越来越重要的作用。

在进行序列分析之前，需要先选择合适的引物，以确保检测到所需的目标序列，并避免检测到不必要的杂交或引物间的相互杂交。

本文将介绍主要使用的引物及其序列。

1. 基因测序引物基因测序引物是用于测序DNA或RNA的引物。

这些引物通常包括一个DNA或RNA序列和一个与之匹配的引物序列。

常用的基因测序引物包括以下几种：- M13引物M13引物是一种通用的测序引物，用于测序单链DNA。

其序列为：5'-GTAAAACGACGGCCAGT-3'。

- T7和SP6引物T7和SP6引物用于测序DNA文库中的克隆DNA。

其中T7引物的序列为：5'-TAATACGACTCACTATAGGG-3'，SP6引物的序列为：5'-ATTTAGGTGACACTATAG-3'。

- M13F和M13R引物M13F和M13R引物也用于测序单链DNA。

M13F引物的序列为：5'-GTAAAACGACGGCCAGT-3'，M13R引物的序列为：5'-CAGGAAACAGCTATGAC-3'。

- T3和T7引物T3和T7引物与RNA兼容，并用于测序RNA和RNA-DNA杂交分子。

T3引物的序列为：5'-AATTAACCCTCACTAAAGGGA-3'，T7引物的序列为：5'-TAATACGACTCACTATAG-3'。

2. PCR引物PCR引物是用于荧光定量PCR、数字PCR、标准PCR等技术的引物。

以下是几种常用的PCR引物：- GAPDH引物GAPDH引物用于检测人体组织中的GAPDH基因表达水平。

其序列为：5'-GAAGGTGAAGGTCGGAGT-3'（前引物）、5'-GAAGATGGTGATGGGATTTC-3'（后引物）。

载体及通用引物

pCANTB5E
S1
S6
pCAT3-enhancer
RVP3
此载体无反向引物
pCDFDuet-1(MCSI)
ACYCDuetUP1 Primer
DuetDOWN1
pCDFDuet-1(MCSII)
DuetUP2
T7TER
pcDNA1.1
CMV-Profor,T7
pCDM8-3
pcDNA2.1
M13for/T7
EGFP-Nrev/ PEGFP-N-3′
pEGFP-C
PEGFP-C-5′
PEGFP-C-3′
pEGFP-C1
EGFP-Cfor
SV40-pArev
pEGFP-C2
EGFP-Cfor
SV40-pArev
pEGFP-C3
EGFP-Cfor
SV40-pArev
pEGFP-F
EGFP-Cfor
SV40-pArev
T7
T7-Terminator
pET-25b(+)
T7
T7-Terminator
pET-26b(+)
T7
T7-Terminator
pET-27b(+)
T7
T7-Terminator
pET-28a(-b,-c)(+)
T7
T7-Terminator
pET-29a(-b,-c)(+)
S.tag/T7
T7-Terminator
T7-Terminator
pET-20b(+)
T7
T7-Terminator
pET-21(-a,-b,-c,-d)(+)
T7

常见载体引物表

P2(pCAMBIA 1301)
pMIR-report
M13F
PSI-CHECK2
PSI-CHECK2-F
PSICHECF-2-R1
PLK0.1
LK0.1-5
p3xFlag-CMV-7.1
CMV-F
CMV-F
无
pDSRED-PRESS-C1
pDSRED -C1-F
pDSRED-C1-R
pCR-Blunt
M13R
M13F/T7
pMT/V5-His_A (_B,_C)
MT-Profor
BGHrev
pEASY-T3
T7/M13F
SP6/M13R
PSILENCE-1.0-U6
T7
2.0REV(同P2(pCAMBIA 1301))
T7/S.tag
T7 Ter
pET-42a (-b, -c)(+)
Stag
T7 Ter
pET50(amp+)
T7/s.tag
T7TER
pET44(AMP+)
T7/s.tag
T7 Ter
pEYFP-N1
pEGFP-N-5’
pEGFP-N-3’
PETBlue
PETBlueT7UP(太靠前，在T7前)/ PETBlueUP
常见载体引物表
Vector
Primer(F)
Primer(R)
PBAD
PBAD-F/TRXF
PBAD-R
pACT
T7
T3
pAc5.1/V5-His
Ac5-Profor
BGH
pACT2
GAL4-AD-Cfor
pGAL4-ADrv
pAS2-1

常用载体构建说明书

常用载体构建说明书步骤：一、基本耗材准备（抗生素、LB液体培养基、LB固体培养基、离心管、枪头、三角瓶）二、制备感受态大肠杆菌三、设计引物四、Pcr扩增五、Pcr产物检测六、Pcr产物回收七、双酶切pcr产物和质粒八、酶切产物回收九、目的基因与载体连接十、转化感受态大肠杆菌十一、单克隆检测十二、测序比对十三、提质粒十四、酶切验证十五、转化感受态农杆菌十六、单克隆检测十七、侵染液配制一、基本耗材准备1、抗生素的制备（抗生素为索来宝公司）常用抗生素Kan（卡那）Amp（氨苄）Rif（利福平）母液浓度：Kan 50mg/ml Amp 100 mg/ml Rif 100 mg/ml工作浓度：Kan 50ng/ul Amp 100 ng/ul Rif 100 ng/ul举例：称取kan固体1g 于注射器中，加入20ml ddH2O，溶解后用过滤器注入灭过菌的离心管中，-20度保存，使用比例1:1000注意：Rif溶解时加入DMSO2、培养基的配制LB液体培养基1000ml 200ml牛肉膏5g 1g蛋白胨10g 2g氯化钠10g 2gLB固体培养基1000ml 200ml牛肉膏蛋白胨氯化钠琼脂粉5g10g10g15g1g2g2g3g120℃高温高压灭菌15-20min，固体培养基冷却后加入相应抗性，加入比例1:1000，然后把培养基倒90cc的培养皿中，一般情况下一个培养皿可倒20ml培养基。

3、离心管、枪头、三角瓶0.2ml、1.5ml、2.0ml离心管各200-500个，50ml离心管2个10ul、200ul、1000ul 枪头各2盒50ml、100ml、250ml 、500ml三角瓶各2个去离子水或双蒸水500ml二、制备感受态大肠杆菌（所用超级感受态细胞制备试剂盒购于上海生工）BT Media 培养基制备：1支BT Media加50 ml蒸馏水配制，放入250ml三角瓶，高压灭菌即可准备工作：将BT Buffer A和BT Buffer B 放冰上遇冷，遇冷低温离心机至4℃1、用超低温冰箱中保存的菌种在LB平板上进行划线，置37℃培养箱中静置培养12-16h待菌落生长到1-2mm大小。

常用载体通用引物

pBR322(BamHI-site)
PBRforBam
PBRrevBam
pBR322(EcoRI/HindIII-sites)
pBRforEco
pBRrevHind
pBR325(BamHI-site)
PBRforBam
PBRrevBam
pBR329(BamHI-site)
PBRforBam
PBRrevBam
pDONR223
M13F
M13R
pDrive
T7/M13rev
SP6/M13for
pDsRED1-C1
(DsRed1-N-f)
DsRed1-C-r
pDsRed1-N1
CMV-Profor
RFP-Nrev,CMV-R?
pDsRed2-N1
(DsRed1-N-f)
DsRed1-C-r
pDsRed2-C1
BGH rev
pcDNA6/myc-His(A,B,C)
CMV-Profor,T7
BGH rev,c-mycrev
pcDNA6/V5-His(A,B,C)
CMV-Profor,T7
BGH rev,V5rev
pCEP4
pCEP-F/CMV-Profor
EBVrev
pCI
T7(17BASE)
EBVrev
T7,CMV-Profor
BGH rev,c-mycrev
pcDNA4/TO
TREfor/CMV-Profor
BGH rev
pcDNA4/V5-His(A,B,C)
CMV-Profor,T7
BGH rev,V5rev
pcDNA4-TET/0N/AMP+

常见载体的测序引物

常见载体的测序引物常见载体的测序引物：Primer of Vector:Vector：Primer(F)；Primer(R)pACT T7 T3pACT2 GAL4 AD pACT2-RpAS2-1 GAL4 BD pAS2-1.RpB42AD pB42ADF pB42ADRpBACPAK8 BAC1 BAC2pBK-CMV T7 T3PBS(SK/KS)/M13- M13F/T7 T3/M13RpBV220 PBV220F PBV220RpCAMBIA 1301(1300) P1 P2pCAMBIA 2300 M13R(-48) M13F(-47)PCANTB5E S1/M13R S6pCAT3-enhancer RVP3 此载体无反向引物pcDNA3.0 CMV-F/T7 SP6/BGHpcDNA3.1 T7 BGHpcDNA4 T7/CMV-F BGHpcDNA6 T7/CMV-F(J21025在T7前面〕 BGHpcDNAII T7 SP6pCE2.1 M13F M13RpCEP4 pCEP-F EBV-RpCF-T M13F M13RpCI T7〔17Base〕此载体无反向引物pCI-neo T7〔17Base〕 T3pCMS-EGFP T7 T3pCMV-3Tag-4A T3 /PFLAG-CMV-F T7pCMV5 pCMV5F pCMV5RpCMV5-Flag pCMV5F pCMV5RpCMV-MYC/HA pCMV-F pCMV-RpCMV-Sport M13F/T7 SP6/M13RpCMV-Tag(KAN+) T3 T7pCR2.1-TOPO M13F/T7 M13RpCR3.1 T7 BGHpCS2 SP6 T7pDonar M13F M13RpDONR221 M13F M13RpDrive T7 SP6pDRIveR(KAN+) T7 SP6pDsRED1-C1 pDsRED-ex-C1-F pEGFP-N-3’(距离很近,一般不用) pDsRED2-C1(KAN+) pDsRED-ex-C1-F pEGFP-N-3’pDSRED-N1(KAN+) pEGFP-N-5’ PDSRED-N-RpECFP-C pEGFP-C-5’ pECFP-C-3'pECFP-N1 pEGFP-N-5’ pEGFP-N-3’pEF/myc/ER pEF-F pCDNA3.1RpEGFP-C(KAN+) pEGFP-C-5’ pEGFP-C-3'pEGFP-N(KAN+) pEGFP-N-5’ pEGFP-N-3’pENTR/D-TOPO(KAN+) M13F M13RpET-*(His)(KAN+) T7 T7 TerpET22(a,b,c)(AMP+) T7 T7 TerpET28,30,24,49(KAN+) T7 T7 TerpET32(a,b,c)(AMP+) T7/S.tag T7 TerpET50(amp+) T7/s.tag T7TERpET44(AMP+) T7/s.tag T7 TerpEYFP-N1 pEGFP-N-5’ pEGFP-N-3’pEYFP-C1 pEGFP-C-5’ pEGFP-C-3'pFLAG-CMV pFLAG-CMV -F pFLAG-CMV -RpGAD424 GAL4 AD 3'ADpGADGH GAL4 AD 3'ADpGADT7-Rec GAL4 AD/T7 3'ADpGAPZa-A a-FACTOR 3'AOXpGBKT7(Kana+) T7 3'BDpGEM-T(-Easy) M13F/T7 SP6/M13RpGEX-(*)T pGEX-4T-5’ pGEX-4T-3’pGL2 GLP1 GLP2pGL3 RVP3 GLP2(RVP4)(没有特别说明用GLP2) PinpointTM Vector Pinpoint Primer SP6pIRES PIRES-F/T7 T3/PIRES-R (J40720)’’pLXSN pLXSN-F pLXSN-RpMAL-c2E MalE primer M13F(-47)pMAL-C2x P5’ P3’pMAL-p2X MalE primer M13F(-47)PMD18-T M13R(-48) M13F(-47)PMD19-T M13F(-47) M13R(-48)pPIC9K(AMP+、ZEO+〕 5’AOX/a-factor 3’AOX pPICZa 5'AOX/PPICZa-F 3'AOXpPROEXHTA M13R(-48) 无反向引物pQE30or40 pQE30-F pQE-R:pRSET T7 T7TERpSilencer3.1-H1 hygro M13F(-47) 3.0REVpSILENCEV2.0-U6 T7 2.0REVpSK01-T M13F M13RpSK-CMV(KAN+) T7 T3pSP72 T7 SP6pSport1 SP6/M13F(-47) T7/M13RpSUPER T7 T3pTA2 M13F/T7(优先使用M13F〕 T3/M13RpT-Adv M13F /T7 M13RpTARGET TM pTarget.F(在T7前面〕/T7 pTarget.R pTO-T7 T7 T7TERpTRC99a-c pTRC99C-F pTRC99C-RpTriPLEx2 5'pTriPLEx2 T7pTWIN-1 T7 T7TER(客户确认再用〕pUC18(19)/118(119) M13F(-47) M13R(-48)pUCm-T M13F/T7 M13RpVAX1 T7 BGHpWSK29 T7/M13F/M13F(-47) T3/M13R/M13R(-48)pXT7 T7 SP6pYES2 T7 pYes2.RpLEXA pLEXA-F pLEXA-RpLNCX pLNCX-F pLNCX-RpRECEIVER CMV-F 此载体无反向引物pSHUTTLE-CMV PSHUTTLE-CMV-F PSHUTTLE-CMV-RpSP64/65 SP6 此载体无反向引物pSTBlue-1 T7 SP6pT7Blue(R) T7 M13F(-47)引物名称序列(5'-3')：M13R：CAG GAA ACA GCT ATG ACC。

常见载体引物表

常见载体引物表
Vector
Primer（F）
Primer（R）
PBAD
PBAD—F/TRXF
PBAD—R
pACT
T7
T3
pAc5.1/V5-His
Ac5-Profor
BGH
pACT2
GAL4—AD—Cfor
pGAL4-ADrv
pAS2—1
GAL4 BD
pAS2-1.R
pB42AD
pB42ADF
pB42ADR
M13F(-47）
3。0REV
pSILENCEV2。0-U6
T7
2.0REV
pSK01-T
M13F
M13R
pSK—CMV(KAN+)
T7
T3
pSP72
T7
SP6
pSport1
SP6/M13F（—47）
T7/M13R
pSUPER
T7
T3
pTA2
M13F/T7（优先使用M13F)
T3/M13R
pT—Adv
DuetDOWN1
pDSRED2—N1（KAN+）
pEGFP—N-5
DsRed1—N
pFastBac Dual (MCSI)
BaculoVfor/PH
pFastBac-R/SV40-pArev
pBSN—T
T7
T3
pHISi－1
PHISi—1
无
pRSV—Rev(根据酶切位点选择不同引物）
pREPF/M13F
pLVX-IRES—ZsGreen1
PIRES2—EGFP-F
PIRES2-EGFP-R
pDC316
pDC316F

常用载体及相关通用引物

常用载体及相关通用引物默认分类2008-10-06 13:16:31 阅读512 评论0 字号：大中小载体一端另一端pBlueScriptSK(+) M13F(-77)/M13F(-47)/M13F/T7 M13R(-88)/M13R(-48)/M13R/T3 pcDNA3 H1.3F/pCMV-F /pEGFP-N-5/T7 BGHpcDNA3.1(+) H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.0(+)/myc-His C/A/B H1.3F/pCMV-F /pEGFP-N-5/T7 BGHpcDNA3.1(+)/myc-His C/A/B H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1(+)/myc-His/LacZ H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/His C/A/B H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/His/LacZ H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/Hygro(-) H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/Hygro(+) H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/Hygro/LacZ H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/V5-His C/A/B H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/V5-His/LacZ H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/V5-His-TOPO H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/V5-His-TOPO/LacZ H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/Zeo(-) H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/Zeo(+) H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpcDNA3.1/Zeo/CAT H1.3F/pCMV-F /pEGFP-N-5/T7 pCDNA3.1R/BGHpColdI DNA p-GEX3’pColdII DNA p-GEX3’pColdIII DNA p-GEX3’pColdIV DNA p-GEX3’pDream2.1 H1.3F/pCMV-F /pEGFP-N-5/T7 SP6pEGFP-N1,2,3 H1.3F/pCMV-F /pEGFP-N-5 pEGFP-N-3’/pEGFP-C-5’/pEGFP-C-3' pET15b T7 T7terpET20b T7 T7terpET22b T7 T7terpET28a T7 T7terpET28b T7 T7terpET30b T7 T7terpFastBac Dual pEGFP-C-3'pFastBac HT A,B,C pEGFP-C-3'pFastBac1 pEGFP-C-3'pGEM-T Easy M13F(-77)/M13F(-47)/M13F/T7 M13R(-88)/M13R(-48)/M13RpGEX-4T-1/3 p-GEX5’ p-GEX3’pGS-21a p-GEX5’ T7terpLenti6/V5-D-TOPO M13F(-47)/M13F/T7 M13R(-48)/M13R/T3pLenti6/V5-GW/lacZ M13F(-47)/M13F/T7 M13R(-48)/M13R/T3pMAL-p2x M13F(-47)/M13F/PBV220R M13R(-48)pMD18-T M13F(-77)/M13F(-47)/M13F M13R(-88)/M13R(-48)/M13RpMD19-T M13F(-77)/M13F(-47)/M13F M13R(-88)/M13R(-48)/M13RpMTBiPV5-His GFP p-GEX3’/ M13F(-47)/M13F M13R(-88)/M13R(-48)/M13R /pEGFP-C-3'/BGH pPCR script M13F(-77)/M13F(-47)/M13F/T7 M13R(-88)/M13R(-48)/M13R/T3pPIC3.5 5’AOX 3’AOXpPIC9 5’AOX 3’AOXpPICZ C/A/B 5’AOX 3’AOXpPICZalpha C/A/B 5’AOX/α-Factor 3’AOXpSecTag2 A,B,C H1.3F/pCMV-F /pEGFP-N-5/T7 BGHpTWIN T7 T7terpUC57 M13F(-77)/M13F(-47)/M13F M13R(-88)/M13R(-48)/M13RpUC18 M13F(-47)/M13F M13R(-48)/M13RpUC19 M13F(-47)/M13F M13R(-48)/M13R其他…… ……注：1）排列顺序为：有左到右==（离插入为点）由远到近；2）若有多个引物，红色的为首选引物：3）选择测序通用引物时，以距离插入位点约50碱基为佳；4）同时有T7，T7ter的载体，单反应首选T7ter；5）同时有T7，pCDNA3.1R的载体，单反应首选pCDNA3.1R ；6）同时有T7，BGH的载体，单反应首选BGH；7）同时有pCMV-F，BGH的载体，单反应首选BGH；。

GA引物载体对照表1

AmpR AmpR
AmpR ZeocinR ZeocinR ZeocinR ZeocinR ZeocinR ZeocinR
KanaR
pGEM-1 pGEM-11z pGEM-3 pGEM-3z pGEM-3Zf(+)(-) pGEM-4 pGEM-4Z pGEM-4z pGEM-5z pGEM-5Zf(+)(-) pGEM-7z pGEM-7Zf(+)(-) pGEM-9z pGEM-9Zf(+)(-) pGEM-T pGEM-T pGEM-T_Easy pGEM-Teasy pGEX-4T-1(-2,-3) pGEX-5X-1(-2,-3) pGEX-6P-1(-2,-3) pGL2 pGL2-Basic pGL3(MCSI,luc+upstream) pGL3(MCSII,luc+downstream) pGL3-Basic(MCSI) pGL3-Basic(MCSII) pGL4(MCSI,luc+upstream) pGL4(MCSII,luc+downstream) pIRES(MCSI) pIRES(MCSII) pIRESneo2 pLVX-IRES-ZsGreen1 pMALc PMAL-C2X pMALd pMALe pMB438 Pmd18/19-T pPIC3.5K pPIC6A pPIC6alphaA pPIC6alphaB pPIC6alphaC
AmpR&KanaR AmpR&KanaR AmpR AmpR AmpR&KanaR AmpR&KanaR AmpR AmpR&KanaR
pET-3(-a,-b,-c,-d) pET-30_Ek/LIC pET-30_Xa/LIC pET-30a(-b,-c)(+) pET-31b(+) pET-32a(-b,-c)(+) pET-33b(+) pET-34b(+) pET-35b(+) pET-36b(+) pET-37b(+) pET-38b(+) pET-39b(+) pET-40b(+) pET-41a(-b,-c)(+) pET-42a(-b,-c)(+) pET-44a(-b,-c)(+) pET-44a_EK/LIC pET-45b(+) pET-46_Ek/LIC pET-5(-a,-b,-c) pET-7 pET-9(-a,-b,-c,-d) pETDuet-1(MCSI) pETDuet-1(MCSII) pFastBac-Dual pFastBacDual(MCSI,PHpromoter) pFastBacDual(MCSII,p10promoter) pFastBac-GT pFastBac-HT PFLAG-CMV4 pGADT7 pGADT7 pGAD-T7 pGAPZA pGAPZalphaA pGAPZalphaB pGAPZalphaC pGAPZB pGAPZC pGBD-T7 pGBKT7 pGBKT7

常用载体测序引物列表

常⽤载体测序引物列表常⽤载体测序引物列表载体名称正向引物反向引物pACT T7 T3pACT2 GAL4 AD(5’AD) 3'AD/pACT2-R PACYCDUET-1(MCSII) DuetUP2 T7TERpAD/pl-DEST CMV-FpAD-Track pAD-Track-F ⽆pAS2-1 5’BD M13-26/48 PB42AD PB42ADF PB42ADR PBAD PBAD-F PBAD-R pBABE pBABE5’ pBABE3’ PBACPAK8 BAC1BAC2 pBK-CMV T7/M13-20 T3/M13-26 PBS(SK/KS)/M13- M13F/T7T3/M13R pBV220 PBV220F PBV220R pBI121需根据酶切位点确认需根据酶切位点确认 pCAMBIA 1301(1300)需根据酶切位点确认需根据酶切位点确认 pCAMBIA 2300 需根据酶切位点确认需根据酶切位点确认PCANTB5E S1/M13R S6pCAT3-enhancer pGL3+pcDNA3.0 pEGFP-N5/T7 SP6/BGHpcDNA3.1 pEGFP-N5/T7 BGHpcDNA4 T7/pEGFP-N5 BGHpcDNA6 T7/pEGFP-N5 BGHpcDNAII T7SP6 pCE2.1 M13FM13R pCEP4 pCMV5R/PCEP-F pEGFP-N5/EBV-R pCF-T M13FM13R pCIT7（17Base ） pCMV5R pCI-neo T7（17Base ） T3pCMS-EGFP T7T3 pCMV-3Tag-4A T3 /pFlag-CMV-F T7pCMV5 pCMV5F pCMV5R pCMV5-Flag pCMV5FpCMV5R pCMV-MYC/NUC pCMV5FpCMV5R PCMV-HA pCMV5FpCMV5R pCMV-SportSP6/M13RpCMV-Tag T3T7 pCMV-TNT T7/SP6pCMV5R pCR2.1-TOPO M13F/T7M13R pCR3.1 T7BGH pCS2 SP6T7PDNR-LIB M13F M13RpDonar M13F M13R pDONR201 pDONR-F pDONR-R pDONR221 M13F M13RpDrive T7 SP6 pDRIveR T7 SP6pDsRED1-C1 pDsRED-C1-F pECFP-C-3 pDsRed1-N1 pEGFP-N-5 RFP-NrevpDsRed-Monomer-N1 pEGFP-N-5 pDsRed1-NpDsRED2-C1 pDsRED-ex-C1-F pEGFP-N-3’pEGFP-N-5 pDsRed1-N pDSRED2-N1pDSRED-N1 pEGFP-N-5’ PDSRED-N-R pEASY-BLUNT M13F/T7 M13RTerpEASY-E1 T7 T7pEASY-T1 M13F/T7 M13RpEASY-T3 M13F/T7 M13R/SP6 pECFP-C pEGFP-C-5’ pECFP-C-3' pECFP-N1 pEGFP-N-5’ pEGFP-N-3’pEF/myc/ER pEF-F pCDNA3.1R pEGFP-C pEGFP-C-5’ pEGFP-C-3' pEGFP-N pEGFP-N-5’ pEGFP-N-3’ pENTR/D-TOPO M13F M13RTerpET-*(His) T7 T7pET22(a,b,c) T7 T7TerTerpET28,30,24,49 T7 T7TerpET32(a,b,c) T7/S.tag T7pET-42a (-b, -c)(+) Stag T7 TerpET50 T7/s.tag T7TERpET44 T7/s.tag T7Ter pEYFP-N1 pEGFP-N-5’ pEGFP-N-3’ PETBlue M13-20 PETBlueDOWN PETDUET-1(MCSI) PBRrevBam DuetDOMN1 PETDUET-1(MCSII) DuetUP2 T7TERpEYFP-C1 pEGFP-C-5’ pEGFP-C-3' pEYFP-N1 pEGFP-N-5’ pEGFP-N-3’ pFastBac pFastBac-F pFastBac-R pFastBac HT_A,B,C pFastBac-PH pECFP-C-3 pFastBac1 pFastBac-PH pECFP-C-3 pFastBac Dual (MCSI) pFastBac-PH pECFP-C-3 pFLAG-CMV CMV30/pFLAG-CMV-F CMV-24/pFLAG-CMV-Rp3xFlag-CMV-7.1 CMV-F/CMV24 CMV30pGAD424 5’AD 3'ADpGADGH 5’AD 3'ADpGADT7-Rec 5’AD/T7 3'ADpGAPZa-A/B/C a-FACTOR 3'AOXforward 3'AOXpGAPZ-A/B/C pGAPpGBKT7 T7 3'BDpGEM-T(-Easy) M13F/T7 SP6/M13RpGEX-(*)T pGEX-5’ pGEX-3’pGL2 pCMV5R PGL3-Pgl3-BASIC pGL3+ PGL3-pGL3-Enhancer pGL3+ PGL3-PinPoint TM PinPoint Primer SP6pIRES PIRES-F/T7 T3/PIRES-RpIRES2-EGFP pIRES2-EGFP.P5’ pIRES2-EGFP.P3’ pLenti6/v5-Dest pEGFP-N-5’PLEXA PLEXA-F PLEXA-R PLNCX PLNCX-F PLNCX-R pLXSN pLXSN-F pLXSN-RPjc1-tac T7pJet.1.21.Blunt T7primer M13F(-47) pMAL-c2E MalEP3/PMAL-C2X-R pMAL-C2x P5/PMAL-C2X-Fprimer M13F(-47) pMAL-p2X MalEPMD18-T M13R(-48) M13F(-47) PMD19-T M13F(-47) M13R(-48) pMIR-report M13FPPC86 PPC86-F PPC86-R3’AOXpPIC9K 5’AOX/a-factor3'AOX pPICZa 5'AOX/PPICZa-FpPROEXHTA M13R(-48)pQE30or40 pQE30+ pQE30- pRECEIVER CMV-FpRSET T7 T7TER PRSFDUET-1(MCSI) DuetUP1 DuetDOWN1 PRSFDUET-1(MCSII) DuetUP2 T7TER pShuttle-CMV pShuttle-CMV-F pShuttle-CMV-R PSILENCE-1.0-U6 T7 T3pSilencer3.1-H1 hygro M13F(-47) 3.0revpSILENCEV2.0-U6 T7 2.0revpSK01-T M13F M13RpSK-CMV T7 T3Psos H1PSP64/65 SP6pSP72 T7 SP6T7/M13RpSport1 SP6/M13F(-47)pSTBlue-1 T7 SP6pSUPER T7 T3pT7Blue(R) T7 M13F(-47)pTA2 M13F/T7 T3/M13R/T7 M13RpT-Adv M13FpTARGET TM pTarget-F/T7 arget-RPTHIOHISA,B,C TRX-FORWARD TRX-REVERSE pTO-T7 T7 T7TERPBV220-/PTRC99C-R pTRC99a-c PQE30+/PTRC99C-F pTriPLEx2 PT5/5'TriplEx2 T7pTz57r/t M13+ M13-pTWIN-1 T7 T7TERpUC18(19)/118(119) M13F(-47) M13R(-48)M13F(-47)/M13F pUC57 M13R(-48)/M13RpUCm-T M13F/T7 M13RpVAX1 T7 BGHT3/M13R/M13R(-48) pWSK29 T7/M13F/M13F(-47)pXT7 T7 SP6pYES2 T7 pYes2.R。

常用引物名称

常用引物名称
引物名称序列(5ห้องสมุดไป่ตู้-3')：
M13R：CAG GAA ACA GCT ATG ACC
M13F：TGT AAA ACG ACG GCC AGT
M13F(-47)：CGC CAG GGT TTT CCC AGT CAC GAC M13R(-48)：AGC GGA TAA CAA TTT CAC ACA GGA M13(-96)：CCC TCA TAG TTA GCG TAA CG
常用引物名称引物名称序列(5'-3'): m13r:cag gaa aca gct atg acc m13f:tgt aaa acg acg gcc agt m13f(-47):cgc cag ggt ttt ccc agt cac gac m13r(-48):agc gga taa caa ttt cac aca gga m13(-96):ccc tca tag tta gcg taa cg sp6:att tag gtg aca cta tag t7:taa tac gac tca cta tag gg t7 ter:tgc tag tta ttg ctc agc gg t3:att aac cct cac taa agg ga pgex-4t-5':ggg ctg gca agc cac gtt tgg tg pgex-4t-3':ccg gga gct gca tgt gtc aga gg glp1:tgt atc tta tgg tac tgt aac tg glp2:ctt tat gtt ttt ggc gtc ttc ca
SP6：ATT TAG GTG ACA CTA TAG
T7：TAA TAC GAC TCA CTA TAG GG