常见载体的测序引物

常见载体的测序引物：

Primer of Vector:

Vector：Primer(F)；Primer(R)

pACT T7 T3

pACT2 GAL4 AD pACT2-R

pAS2-1 GAL4 BD pAS2-1.R

pB42AD pB42ADF pB42ADR

pBACPAK8 BAC1 BAC2

pBK-CMV T7 T3

PBS(SK/KS)/M13- M13F/T7 T3/M13R

pBV220 PBV220F PBV220R

pCAMBIA 1301(1300) P1 P2

pCAMBIA 2300 M13R(-48) M13F(-47) PCANTB5E S1/M13R S6

pCAT3-enhancer RVP3 此载体无反向引物pcDNA3.0 CMV-F/T7 SP6/BGH

pcDNA3.1 T7 BGH

pcDNA4 T7/CMV-F BGH

pcDNA6 T7/CMV-F(J21025在T7前面）BGH pcDNAII T7 SP6

pCE2.1 M13F M13R

pCEP4 pCEP-F EBV-R

pCF-T M13F M13R

pCI T7（17Base）此载体无反向引物

pCI-neo T7（17Base）T3

pCMS-EGFP T7 T3

pCMV-3Tag-4A T3 /PFLAG-CMV-F T7

pCMV5 pCMV5F pCMV5R

pCMV5-Flag pCMV5F pCMV5R

pCMV-MYC/HA pCMV-F pCMV-R

pCMV-Sport M13F/T7 SP6/M13R

pCMV-Tag(KAN+) T3 T7

pCR2.1-TOPO M13F/T7 M13R

pCR3.1 T7 BGH

pCS2 SP6 T7

pDonar M13F M13R

pDONR221 M13F M13R

pDrive T7 SP6

pDRIveR(KAN+) T7 SP6

pDsRED1-C1 pDsRED-ex-C1-F pEGFP-N-3’(距离很近,一般不用) pDsRED2-C1(KAN+) pDsRED-ex-C1-F pEGFP-N-3’

pDSRED-N1(KAN+) pEGFP-N-5’ PDSRED-N-R

pECFP-C pEGFP-C-5’ pECFP-C-3'

pECFP-N1 pEGFP-N-5’ pEGFP-N-3’

pEF/myc/ER pEF-F pCDNA3.1R

pEGFP-C(KAN+) pEGFP-C-5’ pEGFP-C-3' pEGFP-N(KAN+) pEGFP-N-5’ pEGFP-N-3’pENTR/D-TOPO(KAN+) M13F M13R

pET-*(His)(KAN+) T7 T7 Ter

pET22(a,b,c)(AMP+) T7 T7 Ter

pET28,30,24,49(KAN+) T7 T7 Ter

pET32(a,b,c)(AMP+) T7/S.tag T7 Ter

pET50(amp+) T7/s.tag T7TER

pET44(AMP+) T7/s.tag T7 Ter

pEYFP-N1 pEGFP-N-5’ pEGFP-N-3’

pEYFP-C1 pEGFP-C-5’ pEGFP-C-3'

pFLAG-CMV pFLAG-CMV -F pFLAG-CMV -R pGAD424 GAL4 AD 3'AD

pGADGH GAL4 AD 3'AD

pGADT7-Rec GAL4 AD/T7 3'AD

pGAPZa-A a-FACTOR 3'AOX

pGBKT7(Kana+) T7 3'BD

pGEM-T(-Easy) M13F/T7 SP6/M13R

pGEX-(*)T pGEX-4T-5’ pGEX-4T-3’

pGL2 GLP1 GLP2

pGL3 RVP3 GLP2(RVP4)(没有特别说明用GLP2)

PinpointTM Vector Pinpoint Primer SP6

pIRES PIRES-F/T7 T3/PIRES-R (J40720)

pIRES2-EGFP pIRES2-EGFP.P5’ pIRES2-EGFP.P3’pLXSN pLXSN-F pLXSN-R

pMAL-c2E MalE primer M13F(-47)

pMAL-C2x P5’ P3’

pMAL-p2X MalE primer M13F(-47)

PMD18-T M13R(-48) M13F(-47)

PMD19-T M13F(-47) M13R(-48)

pPIC9K(AMP+、ZEO+）5’AOX/a-factor 3’AOX pPICZa 5'AOX/PPICZa-F 3'AOX

pPROEXHTA M13R(-48) 无反向引物

pQE30or40 pQE30-F pQE-R:

pRSET T7 T7TER

pSilencer3.1-H1 hygro M13F(-47) 3.0REV pSILENCEV2.0-U6 T7 2.0REV

pSK01-T M13F M13R

pSK-CMV(KAN+) T7 T3

pSP72 T7 SP6

pSport1 SP6/M13F(-47) T7/M13R

pSUPER T7 T3

pTA2 M13F/T7(优先使用M13F）T3/M13R