Invitrogen中国测序通用引物序列

Invitrogen中国测序通用引物序列
引物名称序列(5'-3')
M13R CAG GAA ACA GCT A TG ACC
M13F TGT AAA ACG ACG GCC AGT
M13F(-47) CGC CAG GGT TTT CCC AGT CAC GAC M13R(-48) AGC GGA TAA CAA TTT CAC ACA GGA M13(-96) CCC TCA TAG TTA GCG TAA CG
SP6 A TT TAG GTG ACA CTA TAG
T7 TAA TAC GAC TCA CTA TAG GG
T7 terminator TGC TAG TTA TTG CTC AGC GG
T3 A TT AAC CCT CAC TAA AGG GA pGEX-4T-5' GGG CTG GCA AGC CAC GTT TGG TG pGEX-4T-3' CCG GGA GCT GCA TGT GTC AGA GG GLp1 TGT A TC TTA TGG TAC TGT AAC TG GLp2 CTT TA T GTT TTT GGC GTC TTC CA RVp3 CTA GCA AAA TAG GCT GTC CC
RVp4 GAC GA T AGT CA T GCC CCG CG pcDNA3.1R TAG AAG GCA CAG TCG AGG PinPoint primer CGT GAC GCG GTG CAG GGC G pCMV-F TCT AAA AGC TGC GGA A TT GT pCMV-R TCCAAACTCA TCAA TGTA TC
pTRC99C-F: TTG CGC CGA CA T CA T AAC
pTRC99C-R: CTGCGTTCTGA TTTAA TCTG
pCEP-F: AGA GCT CGT TTA GTG AAC CG
EBV-R : GTG GTT TGT CCA AAC TCA TC pIRES2-EGFP.P5’:GTA GGC GTG TAC GGT GGG AG pIRES2-EGFP.P3’: AAC GCA CAC CGG CCT TA T TC
3'AD: AGA TGG TGC ACG A TG CAC AG
CMV -F CGC AAA TGG GCG GTA GGC GTG
S1 CAA CGT GAA AAA A TT A TT A TT CGC S6 GTA AA T GAA TTT TCT GTA GTA GG
5`AOX1 GAC TGG TTC CAA TTG ACA AGC
3`AOX1 GCA AA T GGC A TT CTG ACA TCC
α-Factor TAC TA T TGC CAG CA T TGC TGC
GAL4 AD TAC CAC TAC AA T GGA TG
pACT2-R GTGCACGA TGCACAGTTGAA
pB42ADF: CCA GCC TCT TGC TGA GTG GAG A TG
pB42ADR: AAG CCG ACA ACC TTG A TT GGA G pEGFP-N-5’: TGG GAG GTC TA T A TA AGC AGA G pEGFP-N-3’: CGT CGC CGT CCA GCT CGA CCA G pEGFP-C-5’: CA T GGT CCT GCT GGA GTT CGT G pEGFP-C-3' : TA T GGC TGA TTA TGA TCA GT
PBV220F: AAG AAG GGC AGC A TT CAA AG
PBV220R: CTG CGT TCT GA T TTA A TC TG
U6 A TG GAC TA T CA T A TG CTT ACC GTA
2.0rev primer: AGG CGA TTA AGT TGG GTA
3.0rev: GAG TTA GCT CAC TCA TTA GGC
S.tag GAA CGC CAG CAC A TG GAC
5'TriplEx2 CTC CGA GA T CTG GAC GAG C
RVP4 GAC GA T AGT CA T GCC CCG CG
RVP3 CTA GCA AAA TAG GCT GTC CC
pQE30-R GTT CTG AGG TCA TTA CTG G
pQE30-F TGA GCG GA T AAC AA T TTC AC
pEF-F TCA AGC CTC AGA CAG TGG TTC pDSRED-N1-R TGA AGC GCA TGA ACT CCT TG pDsRED-express-C1-F TCC CAC AAC GAG GAC TAC AC
pCMV5R A TT A TA GAG GAC ACC TAG TC
pCMV5F TTC CAA AA T GTC GTA A TA AC
P5' TGC GTA CTG CGG TGA TCA AC
P3' CTG CAA GGC GA T TAA GTT GG
BAC2 ACG CAC AGA A TC TAG CGC TT
BAC1 AAC CA T CTC GCA AA T AAA TA
3'BD: TAA GAG TCA CTT TAA AA T TTG TA T AC pTarget.F CCA GGA TTT TCC CAG TCA C pTarget.R GGC TTT ACA CTT TA T GCT TC
T7(17base) ACA TCC ACT TTG CCT TTC TC
pY es2.R TCG GTT AGA GCG GA T GTG
GAL4 BD TCA TCG GAA GAG AGT AG
pAS2-1.R AAG AGT TAC TCA AGA ACA AGA A pFlag-CMV-F: GGT AGG CGT GTA CGG TGG
pFlag-CMV-R: GCA CTG GAG TGG CAA CTT
pIRES-F: CTT ACT GAC A TC CAC TTT GC pIRES.R: CAC TGC A TT CTA GTT GTG GT
P1 CCA GGC TTT ACA CTT TA T GC
P2 GCG A TT AAG TTG GGT AAC GC
pLEXA-F CGT CAG CAG AGC TTC ACC A T
pLEXA-R TAA AAC CTA AGA GTC ACT TT
pLNCX-F AGC TCG TTT AGT GAA CCG TCA GA T CG pLNCX-R ACC TAC AGG TGG GGT CTT TCA TTC CC pLXSN-F CTT GAA CCT CCT CGT TCG AC
pLXSN-R GTT GCT GAC TAA TTG AGA TG pSHUTTLE-CMV-F GGT CTA TA T AAG CAG AGG TG pSHUTTLE-CMV-R GTG GTA TGG CTG A TT A TG A TC AG MalE Primer GGT CGT CAG ACT GTC GA T GAA GCC Invitrogen中国使用的测序载体和引物
Primer of V ector
V ector Primer(F) Primer(R) pACT T7 T3
pACT2 GAL4 AD pACT2-R
pAS2-1 GAL4 BD pAS2-1.R
pB42AD pB42ADF pB42ADR
pBACPAK8 BAC1 BAC2
pBK-CMV T7 T3
PBS(SK/KS)/M13- M13F/T7 T3/M13R
pBV220 PBV220F PBV220R
pCAMBIA 1301(1300) P1 P2
pCAMBIA 2300 M13R(-48) M13F(-47)
PCANTB5E S1/M13R S6
pCA T3-enhancer RVP3 此载体无反向引物pcDNA3.0 CMV-F/T7 SP6/BGH
pcDNA3.1 T7 BGH
pcDNA4 T7/CMV-F BGH
pcDNA6 T7/CMV-F(J21025在T7前面）BGH
pcDNAII T7 SP6
pCE2.1 M13F M13R
pCEP4 pCEP-F EBV-R
pCF-T M13F M13R
pCI T7（17Base）此载体无反向引物
pCI-neo T7（17Base）T3
pCMS-EGFP T7 T3
pCMV-3Tag-4A T3 /PFLAG-CMV-F T7
pCMV5 pCMV5F pCMV5R
pCMV5-Flag pCMV5F pCMV5R
pCMV-MYC/HA pCMV-F pCMV-R
pCMV-Sport M13F/T7 SP6/M13R pCMV-Tag(KAN+) T3 T7
pCR2.1-TOPO M13F/T7 M13R pCR3.1 T7 BGH pCS2 SP6 T7 pDonar M13F M13R pDONR221 M13F M13R pDrive T7 SP6 pDRIveR(KAN+) T7 SP6
pDsRED1-C1 pDsRED-ex-C1-F pEGFP-N-3’(距离很近,一般不用)
pDsRED2-C1(KAN+) pDsRED-ex-C1-F pEGFP-N-3’pDSRED-N1(KAN+) pEGFP-N-5’PDSRED-N-R pECFP-C pEGFP-C-5’pECFP-C-3' pECFP-N1 pEGFP-N-5’pEGFP-N-3’pEF/myc/ER pEF-F pCDNA3.1R pEGFP-C(KAN+) pEGFP-C-5’pEGFP-C-3' pEGFP-N(KAN+) pEGFP-N-5’pEGFP-N-3’pENTR/D-TOPO(KAN+) M13F M13R
pET-*(His)(KAN+) T7 T7 Ter
pET22(a,b,c)(AMP+) T7 T7 Ter
pET28,30,24,49(KAN+) T7 T7 Ter
pET32(a,b,c)(AMP+) T7/S.tag T7 Ter
pET50(amp+) T7/s.tag T7TER
pET44(AMP+) T7/s.tag T7 Ter
pEYFP-N1 pEGFP-N-5’pEGFP-N-3’pEYFP-C1 pEGFP-C-5’pEGFP-C-3' pFLAG-CMV pFLAG-CMV -F pFLAG-CMV -R pGAD424 GAL4 AD 3'AD pGADGH GAL4 AD 3'AD
pGADT7-Rec GAL4 AD/T7 3'AD
pGAPZa-A a-FACTOR 3'AOX
pGBKT7(Kana+) T7 3'BD
pGEM-T(-Easy) M13F/T7 SP6/M13R pGEX-(*)T pGEX-4T-5’pGEX-4T-3’pGL2 GLP1 GLP2
pGL3 RVP3 GLP2(RVP4)(没有特别说明用GLP2)
PinpointTM V ector Pinpoint Primer SP6
pIRES PIRES-F/T7 T3/PIRES-R (J40720) pIRES2-EGFP pIRES2-EGFP.P5’pIRES2-EGFP.P3’pLXSN pLXSN-F pLXSN-R
pMAL-c2E MalE primer M13F(-47)
pMAL-C2x P5’P3’
pMAL-p2X MalE primer M13F(-47)
PMD18-T M13R(-48) M13F(-47)
PMD19-T M13F(-47) M13R(-48)
pPIC9K(AMP+、ZEO+）5’AOX/a-factor 3’AOX
pPICZa 5'AOX/PPICZa-F 3'AOX pPROEXHTA M13R(-48) 无反向引物
pQE30or40 pQE30-F pQE-R:
pRSET T7 T7TER
pSilencer3.1-H1 hygro M13F(-47) 3.0REV
pSILENCEV2.0-U6 T7 2.0REV
pSK01-T M13F M13R
pSK-CMV(KAN+) T7 T3
pSP72 T7 SP6
pSport1 SP6/M13F(-47) T7/M13R
pSUPER T7 T3
pTA2 M13F/T7(优先使用M13F）T3/M13R
pT-Adv M13F /T7 M13R
pTARGET TM pTarget.F(在T7前面）/T7 pTarget.R
pTO-T7 T7 T7TER
pTRC99a-c pTRC99C-F pTRC99C-R pTriPLEx2 5'pTriPLEx2 T7
pTWIN-1 T7 T7TER(客户确认再用）pUC18(19)/118(119) M13F(-47) M13R(-48)
pUCm-T M13F/T7 M13R
pV AX1 T7 BGH
pWSK29 T7/M13F/M13F(-47) T3/M13R/M13R(-48) pXT7 T7 SP6
pYES2 T7 pY es2.R
pLEXA pLEXA-F pLEXA-R
pLNCX pLNCX-F pLNCX-R pRECEIVER CMV-F 此载体无反向引物pSHUTTLE-CMV PSHUTTLE-CMV-F PSHUTTLE-CMV-R pSP64/65 SP6 此载体无反向引物pSTBlue-1 T7 SP6
pT7Blue(R) T7 M13F(-47)。