常见载体引物与序列

3’AOX
3'AOX 无反向引物 pQE-R: T7TER
3.0REV
2.0REV M13R T3 SP6 T7/M13R T3 T3/M13R M13R pTarget.R T7TER pTRC99C-R T7 T7TER(客户确认再用）
M13R(-48)
M13R BGH T3/M13R/M13R(-48) SP6
GTT CTG AGG TCA TTA CTG G TGA GCG GAT AAC AAT TTC AC TCA AGC CTC AGA CAG TGG TTC TGA AGC GCA TGA ACT CCT TG TCC CAC AAC GAG GAC TAC AC ATT ATA GAG GAC ACC TAG TC TTC CAA AAT GTC GTA ATA AC TGC GTA CTG CGG TGA TCA AC CTG CAA GGC GAT TAA GTT GG ACG CAC AGA ATC TAG CGC TT AAC CAT CTC GCA AAT AAA TA TAA GAG TCA CTT TAA AAT TTG TAT AC CCA GGA TTT TCC CAG TCA C GGC TTT ACA CTT TAT GCT TC ACA TCC ACT TTG CCT TTC TC TCG GTT AGA GCG GAT GTG TCA TCG GAA GAG AGT AG AAG AGT TAC TCA AGA ACA AGA A GGT AGG CGT GTA CGG TGG GCA CTG GAG TGG CAA CTT CTT ACT GAC ATC CAC TTT GC CAC TGC ATT CTA GTT GTG GT CCA GGC TTT ACA CTT TAT GC
pDSRED-N1(KAN+) pEGFP-N-5’
pECFP-C
pEGFP-C-5’
pECFP-N1
pEGFP-N-5’
pEF/myc/ER
pEF-F
pEGFP-C(KAN+)
pEGFP-C-5’
pEGFP-N(KAN+)
pEGFP-N-5’
pENTR/DTOPO(KAN+)
M13F
pET-*(His)(KAN+) T7
Invitrogen中国使用的测序载体和引物
Primer of Vector
Vector
Primer(F)
pACT
T7
pACT2
GAL4 AD
pAS2-1
GAL4 BD
pB42AD
pB42ADF
pBACPAK8
BAC1
pBK-CMV
T7
PBS(SK/KS)/M13- M13F/T7
pBV220
PBV220F
GAG TTA GCT CAC TCA TTA GGC
GAA CGC CAG CAC ATG GAC
CTC CGA GAT CTG GAC GAG C
RVP4
GAC GAT AGT CAT GCC CCG CG
RVP3
CTA GCA AAA TAG GCT GTC CC
pQE30-R pQE30-F pEF-F pDSRED-N1-R pDsRED-express-C1pCMV5R pCMV5F P5' P3' BAC2 BAC1 3'BD: pTarget.F pTarget.R T7(17base) pYes2.R GAL4 BD pAS2-1.R pFlag-CMV-F: pFlag-CMV-R: pIRES-F: pIRES.R: P1
ATT AAC CCT CAC TAA AGG GA
GGG CTG GCA AGC CAC GTT TGG TG CCG GGA GCT GCA TGT GTC AGA GG TGT ATC TTA TGG TAC TGT AAC TG CTT TAT GTT TTT GGC GTC TTC CA CTA GCA AAA TAG GCT GTC CC GAC GAT AGT CAT GCC CCG CG TAG AAG GCA CAG TCG AGG CGT GAC GCG GTG CAG GGC G TCT AAA AGC TGC GGA ATT GT TCC AAA CTC ATC AAT GTA TC TTG CGC CGA CAT CAT AAC CTG CGT TCT GAT TTA ATC TG AGA GCT CGT TTA GTG AAC CG GTG GTT TGT CCA AAC TCA TC GTA GGC GTG TAC GGT GGG AG AAC GCA CAC CGG CCT TAT TC AGA TGG TGC ACG ATG CAC AG CGC AAA TGG GCG GTA GGC GTG CAA CGT GAA AAA ATT ATT ATT CGC GTA AAT GAA TTT TCT GTA GTA GG GAC TGG TTC CAA TTG ACA AGC GCA AAT GGC ATT CTG ACA TCC TAC TAT TGC CAG CAT TGC TGC TAC CAC TAC AAT GGA TG GTG CAC GAT GCA CAG TTG AA CCA GCC TCT TGC TGA GTG GAG ATG AAG CCG ACA ACC TTG ATT GGA G TGG GAG GTC TAT ATA AGC AGA G CGT CGC CGT CCA GCT CGA CCA G CAT GGT CCT GCT GGA GTT CGT G TAT GGC TGA TTA TGA TCA GT AAG AAG GGC AGC ATT CAA AG CTG CGT TCT GAT TTA ATC TG ATG GAC TAT CAT ATG CTT ACC GTA AGG CGA TTA AGT TGG GTA
T7
pSport1
SP6/M13F(-47)
pSUPER
T7
pTA2
M13F/T7(优先使用M13F）
pT-Adv
M13F /T7
pTARGET TM
pTarget.F(在T7前面）/T7
pTO-T7
T7
pTRC99a-c
pTRC99C-F
pTriPLEx2
5'pTriPLEx2
pTWIN-1
T7
pUC18(19)/118(119 )
pCMV-Tag(KAN+) T3
pCR2.1-TOPO
M13F/T7
pCR3.1
T7
pCS2
SP6
pDonar
M13F
pDONR221
M13F
pDrive
T7
pDRIveR(KAN+)
T7
pDsRED1-C1
pDsRED-ex-C1-F
pDsRED2-C1(KAN+) pDsRED-ex-C1-F
M13F(-47)
pUCm-T
M13F/T7
pVAX1
T7
pWSK29
T7/M13F/M13F(-47)
pXT7
T7
T7 Ter
T7 Ter
T7TER T7 Ter pEGFP-N-3’ pEGFP-C-3' pFLAG-CMV -R 3'AD 3'AD 3'AD 3'AOX 3'BD SP6/M13R pGEX-4T-3’ GLP2 GLP2(RVP4)(没有特别说明用GLP2) SP6 T3/PIRES-R (J40720) pIRES2-EGFP.P3’ pLXSN-R M13F(-47) P3’ M13F(-47) M13F(-47) M13R(-48)
Invitrogen中国测序通用引物序列
引物名称 M13R M13F M13F(-47) M13R(-48) M13(-96) SP6 T7 T7 terminator
T3
pGEX-4T-5' pGEX-4T-3' GLp1 GLp2 RVp3 RVp4 pcDNA3.1R PinPoint primer pCMV-F pCMV-R pTRC99C-F: pTRC99C-R: pCEP-F: EBV-R : pIRES2-EGFP.P5’: pIRES2-EGFP.P3’: 3'AD: CMV -F S1 S6 5`AOX1 3`AOX1 α-Factor GAL4 AD pACT2-R pB42ADF: pB42ADR: pEGFP-N-5’: pEGFP-N-3’: pEGFP-C-5’: pEGFP-C-3' : PBV220F: PBV220R: U6 2.0rev primer:
pCAMBIA 1301(1300)
P1
pCAMBIA 2300
M13R(-48)
PCANTB5E
S1/M13R
pCAT3-enhancer RVP3
pcDNA3.0
CMV-F/T7
pcDNA3.1
T7
pcDNA4
T7/CMV-F
pcDNA6
T7/CMV-F(J21025在T7前
pcDNAII
T7
pCE2.1
pYES2 pLEXA pLNCX pRECEIVER pSHUTTLE-CMV pSP64/65 pSTBlue-1 pT7Blue(R)
T7 pLEXA-F pLNCX-F CMV-F PSHUTTLE-CMV-F SP6 T7 T7
pYes2.R pLEXA-R pLNCX-R 此载体无反向引物 PSHUTTLE-CMV-R 此载体无反向引物 SP6 M13F(-47)
pFLAG-CMV -F
pGAD424
GAL4 AD
pGADGH
GAL4 AD
pGADT7-Rec
GAL4 AD/T7
pGAPZa-A
a-FACTOR
pGBKT7(Kana+)
T7
pGEM-T(-Easy)
M13F/T7
pGEX-(*)T
pGEX-4T-5’
pGL2
GLP1
pGL3
RVP3
PinpointTM Vector Pinpoint Primer
pIRES
PIRES-F/T7
pIRES2-EGFP
pIRES2-EGFP.P5’
pLXSN
pLXSN-F
pMAL-c2E
MalE primer
pMAL-C2x
P5’
pMAL-p2X
MalE primer
PMD18-T
M13R(-48)
PMD19-T
M13F(-47)
pPIC9K(AMP+、 ZEO+）
pET22(a,b,c)(AMP+ )
T7
Primer(R) T3 pACT2-R pAS2-1.R pB42ADR BAC2 T3 T3/M13R PBV220R
P2
M13F(-47) S6 此载体无反向引物 SP6/BGH BGH BGH BGH SP6 M13R EBV-R M13R 此载体无反向引物 T3 T3 T7 pCMV5R pCMV5R pCMV-R SP6/M13R T7 M13R BGH T7 M13R M13R SP6 SP6 pEGFP-N-3’(距离很近,一般不用) pEGFP-N-3’ PDSRED-N-R pECFP-C-3' pEGFP-N-3’ pCDNA3.1R pEGFP-C-3' pEGFP-N-3’
3.0rev:
S.tag
5'TriplEx2
序列(5'-3') CAG GAA ACA GCT ATG ACC TGT AAA ACG ACG GCC AGT CGC CAG GGT TTT CCC AGT CAC GAC AGC GGA TAA CAA TTT CAC ACA GGA CCC TCA TAG TTA GCG TAA CG ATT TAG GTG ACA CTA TAG TAA TAC GAC TCA CTA TAG GG TGC TAG TTA TTG CTC AGC GG
M13R
T7 Ter
T7 Ter
pET28,30,24,49(KA N+)
T7
pET32(a,b,c)(AMP+ )
T7/S.tag
pET50(amp+)
T7/s.tag
pET44(AMP+)
T7/s.tag
pEYFP-N1
pEGFP-N-5’
pEYFP-C1
pEGFP-C-5’
pFLAG-CMV
M13F
pCEP4
pCEP-F
pCF-T
M13F
pCI
T7（17Base）
pCI-neo
T7（17Base）
pCMS-EGFP
T7
pCMV-3Tag-4A
T3 /PFLAG-CMV-F
pCMV5
pCMV5F
pCMV5-Flag
pCMV5F
pCMV-MYC/HA
pCMV-F
pCMV-Sport
M13F/T7
5’AOX/a-factor
pPICZa
5'AOX/PPICZa-F
pPROEXHTA
M13R(-48)
pQE30or40
pQE30-F
pRSET
T7
pSilencer3.1-H1 hygro
M13F(-47)
pSILENCEV2.0-U6 T7
pSK01-T
M13F
pSK-CMV(KAN+)
T7
pSP72