常用引物信息列表

3’AOX5′-GGCAAATGGCATTCTGACAT-3‘

3‘AD5′- AGA TGG TGC ACG ATG CAC AG-3‘

3‘BD5′-TAA GAG TCA CTT TAA AAT TTG TAT C-3‘5’AOX5′-GACTGGTTCCAATTGACAAGC-3‘

A-FACTOR5′-TAC TAT TGC CAG CAT TGC TGC-3‘

CMV-F5′-CGCAAATGGGCGGTAGGCGTG-3‘

CMV-R5′-GTTCACGGTGCCCTCC-3‘

EGFP-Nrev5′-CGT CGC CGT CCA GCT C-3‘

GLP15′-TGT ATC TTA TGG TAC TGT AAC TG-3‘GLP25′-CTT TAT GTT TTT GGC GTC TTC CA-3‘

M13(-96)5′-CCCTCATAGTTAGCGTAACG-3‘

M13-205′-GTA AAA CGA CGG CCA GTG-3‘

M13F5′-TGTAAAACGACGGCCAGT-3‘

M13F（-47）5′-CGCCAGGGTTTTCCCAGTCACGAC-3‘

M13F-215′-TGT AAA ACG ACG GCC AGT-3‘

M13R5′-CAGGAAACAGCTATGACC-3‘

M13R（-48）5′-AGCGGATAACAATTTCACACAGGA-3‘

P125845′-TTTTCAGTATCTACGAT-3’

P171105′-TACCACTACAATGGATG-3’

pbd-gal4-f5′-GCCTCTAACATTGAGACAGC-3‘

pbd-gal4-r5′-AAGAGTTACTCAAGAACAAGAA-3‘

pbi-121(35s)5′-GACGCACAATCCCACTATCC-3‘

PBV220F5′-AAGAAGGGCAGCATTCAAAG-3‘

PBV220R5′-CTG CGT TCT GAT TTA ATC TG-3‘

PCDNA3.0-R25′-GGC AAC TAG AAG GCA CAG TC-3‘PCDNA3.1F5′-CTAGAGAACCCACTGCTTAC-3’

PCDNA3.1R5′-TAGAAGGCACAGTCGAGG-3’

PCMV-F5′-TCTAAAAGCTGCGGAATTGT-3‘

PCMV-5F5′-TTCCAAAATGTCGTAATAAC-3‘

PCMV-R5′-TCCAAACTCATCAATGTATC-3‘

PCMV-5R5′-ATTATAGAGGACACCTAGTC-3‘

PEGFP-C-3’5′-TATGGCTGATTATGATCAGT-3‘

PEGFP-C-5’5′-CATGGTCCTGCTGGAGTTCGTG-3‘

PEGFP-N-3’5′-CGTCGCCGTCCAGCTCGACCAG-3‘

PEGFP-N-5’5′-TGGGAGGTCTATATAAGCAGAG-3‘

PGEX-3’5′-CCGGGAGCTGCATGTGTCAGAGG-3‘

PGEX-5’5′-GGGCTGGCAAGCCACGTTTGGTG-3‘PIRES2-EGFP-F5′-GTA GGC GTG TAC GGT GGG AG-3‘PIRES3-EGFP-R5′-AAC GCA CAC CGG CCT TAT TC-3‘

pMAL-C2X-F5′-TGC GTA CTG CGG TGA TCA AC-3’

pMAL-C2X-R5′-CTG CAA GGC GAT TAA GTT GG-3’

PQE30F5′- TGA GCG GAT AAC AAT TTC AC-3‘

PQE30R5′- GTT CTG AGG TCA TTA CTG G-3‘

RVP35′-CTA GCA AAA TAG GCT GTC CC-3‘

RVP45′-GAC GAT AGT CAT GCC CCG CG-3‘

S.TAG5-CGAACGCCAGCACATGGACA-3‘

SP65-ATTTAGGTGACACTATAG-3‘

T35-ATTAACCCTCACTAAAGGGA-3‘

T75‘-TAATACGACTCACTATAGGG-3‘

T7（17BASE）5‘-ACATCCACTTTGCCTTTCTC-3‘

T7TER5‘-TGCTAGTTATTGCTCAGCGG-3‘

NOSR AAT CAT CGC AAG ACC GGC

M13 Forward (-20) 5'-GTA AAA CGA CGG CCA GTG-3'

M13 Reverse (-27) 5'-GGA AAC AGC TAT GAC CAT G-3'

M13 Forward (-47) 5'-CGCCAGGGTTTTCCCAGTCACGAC-3'

M13 Reverse (-48) 5'-AGC GGA TAA CAA TTTC ACA C-3'

SP6 5'-TAC GAT TTA GGT GAC ACT ATA G-3'

T3 5'-CAA TTA ACC CTC ACT AAA GG-3'

T7 5'-TAA TAC GAC TCA CTA TAG GG-3'

T7Term 5'-GCT AGT TAT TGC TCA GCG G-3' pBluescript KS 5'-TCG AGG TCG ACG GTA TC-3' pBluescript SK 5'-CGC TCT AGA ACT AGT GGA TC-3'

3'pGEX 5'-CCG GGA GCT GCA TGT GTC AGA GG-3' 5'pGEX 5'-GGG CTG GCA AGC CAC GTT TGG TG-3' GST-Tag 5'-ACC CAA TGT GCC TGG ATG CG-3' pTrcHis-Forward 5'-GAG GTA TAT ATT AAT GTA TCG-3' pTrcHis-Reverse 5'-GAT TTA ATC TGT ATC AGG

CMV-Forward 5'-CGC AAA TGG GCG GTA GGC GTG-3' CMV-Reverse 5'-AGT AGG AAA GTC CCG TAA GG-3' EGFP-C 5'-CAT GGT CCT GCT GGA GTT CGT G-3' EGFP-N 5'-CGT CGC CGT CCA GCT CGA CCA-3' BGH-Reverse 5'-TAG AAG GCA CAG TCG AGG-3' pQEproseq 5'-CCC GAA AAG TGC CAC CTG-3' pQErevseq 5'-GTT CTG AGG TCA TTA CTG G-3'

Intein Forward 5'-CCC GCC GCT GCT TTT GCA CGT GAG-3' 5'-pBabe-Seq 5'-CTT TAT CCA GCC CTC AC-3'

3'-pBabe-Seq 5'-ACC CTA ACT GAC ACA CATT CC-3'

-96 glll Sequencing Primer 5'-CCC TCA TAG TTA GCG TAA CG-3'

GAL1 Forward 5'-AAT ATA CCT CTA TAC TTT AAC GTC-3' pBAD Forward 5'-ATG CCA TAG CAT TTT TAT CC-3'

pBAD Reverse 5'-GATTTAATCTGTATCAGG

pTRE 3' 5'-CCA CAC CTC CCC CTG AAC-3'

pTRE 5' 5'-CGC CTG GAG ACG CCA TCC-3' pYESTrp Forward 5'-GAT GTT AAC GAT ACC AGC C-3' pYESTrp Reverse 5'-GCG TGA ATG TAA GCG TGA C-3' RVprimer3 5'-CTA GCA AAA TAG GCT GTC CC-3' Rvprimer4 5'-GAC GAT AGT CAT GCC CCG CG-3' GLprimer 1 5'-TGT ATC TTA TGG TAC TGT AAC TG-3' GLprimer 2 5'-CTT TAT GTT TTT GGC GTC TTC CA-3' SeqL-A (ATTL1) 5'-GCG AGA GTA GGG AAC TGC-3'