GST标签蛋白纯化全面解决方案
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GST
Sepharose
51
Glutathione Sepharose
GST
Glutathione Sepharose GST
GST
51Glutathione Sepharose
SH Sepharose
Sepharose
GST
GST标签蛋白纯化全面解决方案-纯化篇
GST
GST
16
Glutathione Sepharose
2
16GST
Sepharose High Performance Glutathione Sepharose 4
Sepharose 4
B
Glutathione Sepharose 4B
FF
4B
25ml 100ml
25ml 100ml 100ml
300ml
2ml
5ml
rGST
10mg rGST
10mg GST
(rGST)
(rGST)
rGST
50mg rGST
2
Glutathione Sepharose 4B
Sepharose 4Fast Flow
96
Sepharose 4B
Glutathione Sepharose High Performance
FF16/10
GST
GST
/
5ml
5ml
HRP GST GST
3000cm2
/50mg rGST
>25mg (GST)
rGST
1:5000
A
ECL Plus B
Sepharose 4Fast
Flow
Sepharose 4B
Sepharose 4Fast
GST ECL
PreScission
Xa
GST GST
HRP
GST
Protease
Benzamidine FF High
Sub
Benzamidine Sepharose 4Fast Flow High
Sub
/
5ml
25ml
/
90%100g GST 1mM EDTA 1mM
DTT
150mM
,50mM Tris-(pH7.0)5°C
16
90%100g GST
22°C
1×PBS
16
90%100g
GST
1mM 100mM 50mM Tris-(pH8.0)22°C
175mg
35mg
/
Protease
Benzamidine Sepharose4Fast Flow High Sub
Phe-Gln-Gly-Pro
Gln
Gly
GST
PreScssion
pGEX-6P
GST
pGEX-T
GST
Xa
pGEX-X
GST
Xa
Xa
GST
1mg/l
10mg/l 17 2.5mg/l
17
Glutathione
Sepharose
10ml
2ml
200
l
10l
0.45μm DNase
RNase
GST
Glutathione Sepharose
GST
HiTrap Desalting 5ml PD-10Desalting HiPrep 26/10Desalting
20-
50mM
15mM pH 6.58
SDS-PAGE
GST
SDS-PAGE
1-
-2
4-CDNB
GST
GST
Glutathione Sepharose
GST
GST
Lowry BCA
Bradford Lowry BCA
HiTrap Desalting 5ml
2000
PBS
Bradford
HiTrap
GST
GST
GST MultiTrap FF
GST MultiTrap 4B 960.5mg
GST 50
l Glutathione Sepharose
4B GST SpinTrap
400g
GST GST GSTrap
GSTPrep FF 16/10GSTrap
1ml
5ml GSTPrep FF 16/10
20ml
Glutathione Sepharose
ÄKTAprime plus
GST
UV
ÄKTApuri er
ÄKTAexplorer
法检
测提取物和纯化过程中样品的GST标签蛋白的浓度。