NEB公司提供的保护碱基序列表
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/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized
Table. Cleavage Efficiency Close to the Termini of PCR Fragments
Enzyme
Bp from the recognition site to fragment end
1 2 3 4
5
AanI (PsiI)0 0-20 20-50 AarI
20-50 50-100
AasI (DrdI)50-100
AatII
0-20 20-50 50-100
Acc65I (Asp718I)0-20 50-100 AdeI (DraIII)50-100
AjiI (BmgBI)50-100
AluI
0-20 20-50 50-100
Alw21I (BsiHKAI)50-100
Alw26I 50-100
Alw44I 0 20-50 50-100
ApaI
50-100
BamHI 50-100
BauI (BssSI)
0-20 20-50 50-100
BclI
50-100
BcnI (NciI)20-50 50-100 BcuI (SpeI)50-100
BfiI (BmrI)50-100
BfmI (SfcI)50-100
BfuI (BciVI)50-100
BglI20-50 50-100
BglII0 50-100
Bme1390I (ScrFI)20-50 50-100 BoxI (PshAI)0 50-100
BpiI (BbsI)50-100
Bpu10I20-50 50-100
Bpu1102I (BlpI)50-100
BseDI (BsaJI)0 50-100
BseGI (BtsCI)50-100
BseJI (BsaBI)0 50-100
BseLI (BslI)0 50-100
BseMI (BsrDI)0-20 50-100
BseMII (BspCNI)50-100
BseNI (BsrI)0 50-100
BseSI (Bme1580I)50-100
BseXI (BbvI)20-50 50-100
Bsh1236I (BstUI)50-100
Bsh1285I (BsiEI)0-20 50-100
BshNI (BanI)50-100
BshTI (AgeI)20-50 50-100
Bsp119I (BstBI)50-100
Bsp120I (PspOMI)20-50 50-100
Bsp1407I (BsrGI)20-50 50-100
Bsp143I (Sau3AI)50-100
Bsp68I (NruI)0 50-100
BspLI (NlaIV)50-100
BspOI (BmtI)0 20-50 50-100 BspPI (AlwI)0 50-100
BspTI (AflII)0 0-20 50-100 Bst1107I (BstZ17I)0-20 50-100
BstXI0 50-100 Bsu15I (ClaI)50-100
BsuRI (HaeIII)0-20 20-50 50-100
BveI (BspMI)0-20 50-100
CaiI (AlwNI)0 0-20 50-100
Cfr10I (BsrFI)20-50 50-100
Cfr13I (Sau96I)50-100
Cfr42I (SacII)50-100
Cfr9I (XmaI)20-50 50-100
CfrI (EaeI)0 50-100
CpoI (RsrII)50-100
CseI (HgaI)50-100
Csp6I (CviQI)50-100
DpnI50-100
DraI0 0-20 50-100
Eam1104I (EarI)0 50-100
Eam1105I (AhdI)0 50-100
Ecl136II (EcoICRI)50-100
Eco105I (SnaBI)20-50 50-100
Eco130I (StyI)0 50-100
Eco147I (StuI)0 50-100
Eco24I (BanII)50-100
Eco31I (BsaI)20-50 50-100
Eco32I (EcoRV)20-50 50-100
Eco47I (AvaII)50-100
Eco47III (AfeI)0 0-20 50-100
Eco52I (EagI)0-20 50-100
Eco57I (AcuI)50-100
Eco57MI50-100
Eco72I (PmlI)0-20 50-100
Eco81I (Bsu36I)50-100
Eco88I (AvaI)50-100
Eco91I (BstEII)20-50 50-100
EcoO109I (DraII)50-100
EcoRI50-100
EcoRII0 0-20 20-50 50-100 EheI (SfoI)20-50 50-100
Esp3I (BsmBI)50-100
FaqI (BsmFI)0-20 50-100