切向流过滤装置介绍
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典型切向流超滤装置，使用及操作条件
Ultrafiltration Devices超滤装置
Cassettes (Pellicon XL and Pellicon 2)盒式 (Pellicon XL and Pellicon 2) − Flat sheet device with平板式 − fine screened channel (1-100 kD)致密筛网 (1-100 kD) − coarse screened channel (100-1000 kD)粗糙筛网 (100-1000 kD)
– Flat plate device with coarse screened channel 带有粗糙筛网的平板
– Series/parallel configuration to limit cross flow
requirements 系列配置限定了切向流速的要求
All thermoplastic construction eliminates
adhesives, limits extractables
– 热塑性结构无粘合剂，不易裂开
– 可耐受溶媒 (仅限纤维素材料的UF膜)
– 从20平方英尺线形放大
– 最小工作体积为5L
MILLIPORE
17
Typical TFF Devices, Uses and Operating Conditions
Absolute linear scalability at all sizes – 各孔径呈现绝对线形放大
Smallest minimum working volumes at all sizes – 最小的最小工作体积
MILLIPORE
12
Typical TFF Devices, Uses and Operating Conditions
Î use open channel to prevent plugging if total suspended
solids >2-5% by volume Î 如果固状悬浊物总量大于2-5%, 使用开放流道型防止堵
塞
Availability of chosen membrane可选用相应的膜 usually available in only 1 or 2 devices通常只有一两种超滤装
MILLIPORE
4
Microporous (MF) TFF Devices 切向流微滤装置(MF)TFF
MILLIPORE
5
TFF Devices, Uses and Operating Conditions
切向流(微滤)装置,使用及操作条件
Microporous (MF) TFF Devices
− suspended (open) screen channel (viscous solutions) 悬浮 (开放型) 筛网 (粘性溶液)
Highest efficiency (highest flux at given
cross flow velocity in feed channel) – 高效率 (在固定的切向流速下表现最高的通透量)
MILLIPORE
16
Typical TFF Devices, Uses and Operating Conditions
典型切向流超滤装置，使用及操作条件
Ultrafiltration Devices超滤装置
ÎThermoplastic flat plate (Prostak-UF) 热塑性平板式 (Prostak-UF)
– Aseptic applications灭菌应用
MILLIPORE
6
Microporous (MF) TFF Devices – Prostak 切向流微滤装置 - Prostak
Permeate
透过
Retentate回流
, Uses and Operating Conditions
切向流微滤装置(MF)
ÎGlass Reinforced (PES) flat plate (Prostak)
改良聚醚砜外壳平板膜 (Prostak)
– Flat plate, open flow channel平板式,开放流道
– (SIP) Steamable in place(SIP) 在线蒸汽灭菌
典型切向流超滤装置，使用及操作条件
Applications for Pellicon UF cassettes: PELLICON UF 盒式超滤装置的应用: High-value, low-to-medium volume product concentration / diafiltration, where high recovery required at small final volumes 高价值、低产量产品的浓缩及透析, 浓缩后最终体积较小时要求 保证良好的蛋白收率 – Where compact systems needed due to floor space restrictions 由于可放置设备的空间有限，紧凑型的超滤设备为最佳选择。
置可选
Mechanical complexity, reliability and ease-of-use构造复杂,可 靠但使用简单
Simplicity of scale-up线形放大的简单性
Cost (device AND operating system)成本(设备本身及相应操
作系统)
MILLIPORE
18
Typical TFF Devices, Uses and
– Bacterial, yeast, mammalian cMeIlLlLsI细PO菌RE，酵母和哺乳动物细胞
10
Ultrafiltration (UF) TFF Devices 切向流超滤装置（TFF）
MILLIPORE
11
Typical TFF Devices, Uses and Operating Conditions
TFF Devices切向流过滤装置
Basic Concepts of Tangential Flow Filtration (TFF) 切向流过滤的基本概组件(TFF)
MILLIPORE
1
Outline概要
Types of TFF Devices切向流过滤装置的种类 Îopen channel开放型流道 Îturbulence-promoted channels湍流增强型流道 Î"enhanced" by secondary flow次流道加强型
MILLIPORE
9
TFF Devices, Uses and Operating Conditions
切向流装置,使用及操作条件
Microporous TFF Devices切向流微滤
装置
ÎHollow fibers中空纤维
– Open flow channel (diameters vary)开放流道 (孔的直径大小各异)
典型切向流超滤装置，使用及操作条件
Prostack UF 的应用:
– High-value, medium-to-high volume product 高价值、高产量的产品
– Solvent-containing streams含溶媒的溶液
– Where cassette filter costs become too high 当盒式超滤膜成本太高时
Spiral-wound (screen
between flat sheets wound in
a spiral pattern)
卷式膜(卷起的膜每层之间有筛网)
MILLIPORE
3
TFF Device Selection-Considerations 超滤装置的选择-
从全面考虑的角度进行
Suspended solids in feed含悬浊的固状物进料
Maximum suspended solids
<5% to avoid plugging 最大悬浮固状物的量<5% 避免堵塞孔道
MILLIPORE
8
Microporous (MF) TFF Devices – Pellicon
切向流微滤装置 - Pellicon
Feed进口
Permeate透过
Retentate回流
Where very fast, easy scale-up needed 当要求快速、简单地实现线形放大时
MILLIPORE
13
Millipore提供从研发到生产规模的全 套超滤装置
搅拌式超滤杯 Labscale
Cogent M
离心式超滤管
Cogent SV
Lab
Pilot
Production
MILLIPORE
Stacks of Pellicon 2
20 cm 10 cm each way
cassette - from 0.5
from center to 80m² per holder
MILLIPORE
15
Millipore超滤系统真正实现线性放大
0.1m²
800x
80m²
Pellicon
Cassettes
from center
20 cm 10 cm each way
from center
Pellicon XL50 - 0.05 m²
Pellicon 2 “Mini” - 0.1 m²
20 cm 10 cm each way
from center
Pellicon 2 cassette - 0.5 m²
Open channel 开放流道
Flat plates (space between
membranes)平板 (膜中间有空
隙)
Hollow fibers and
tubes中空纤维及管 Turbulence-promoted channel 湍流增强型流道
Screen between flat
sheets/plates每层平板之间有筛网
– Limited burst pressures (25-50 psi)可承受的
压力有限 (25-50 psi)
– Steamability varies蒸汽灭菌性不稳定
– Minimum volumes vary widely 最小工作体积变化大
ÎApplications:应用:
– Cell harvest and clarification细胞收集和澄清
切向流(微滤)装置,使用及操作条件
Microporous TFF Devices
切向流微滤装置
Î Cassettes (Pellicon)盒式膜包 (Pellicon) – Flat sheet, 20-mesh screened channel平板式,20-MESH筛网 – Not steamable不能蒸汽灭菌
14
线性放大
All flow paths are identical in length, width, height and spacer & are fed in parallel.
Legend
Feed Flow Path Permeat Flow Path
20 cm 10 cm each way
– Minimum volumes > 5 L
– 最小工作体积 > 5 L
Î Applications:应用
– Cell harvest and clarification细胞收集和澄清
– Bacterial, yeast, mammalian cells细菌,酵母,
哺乳动物细胞
– Low shear applications低剪切力应用
– Lower minimum volumes than Prostak 比PROSTAK膜的残留体积更小
Î Applications:应用:
Clarification or bacterial cell harvest 澄清或细菌,细胞收集 - When minimum volumes
must be 100 mL-5 L 处理量范围 100 mL-5 L
Millipore's devices and their performance密理博公司 TFF装置及其性能
Recommended operating conditions for Millipore's TFF devices密理博公司超滤装置的建议操作条件
MILLIPORE
2
Types of TFF Devices TFF装置的类型
Ultrafiltration Devices超滤装置
Cassettes (Pellicon XL and Pellicon 2)盒式 (Pellicon XL and Pellicon 2) − Flat sheet device with平板式 − fine screened channel (1-100 kD)致密筛网 (1-100 kD) − coarse screened channel (100-1000 kD)粗糙筛网 (100-1000 kD)
– Flat plate device with coarse screened channel 带有粗糙筛网的平板
– Series/parallel configuration to limit cross flow
requirements 系列配置限定了切向流速的要求
All thermoplastic construction eliminates
adhesives, limits extractables
– 热塑性结构无粘合剂，不易裂开
– 可耐受溶媒 (仅限纤维素材料的UF膜)
– 从20平方英尺线形放大
– 最小工作体积为5L
MILLIPORE
17
Typical TFF Devices, Uses and Operating Conditions
Absolute linear scalability at all sizes – 各孔径呈现绝对线形放大
Smallest minimum working volumes at all sizes – 最小的最小工作体积
MILLIPORE
12
Typical TFF Devices, Uses and Operating Conditions
Î use open channel to prevent plugging if total suspended
solids >2-5% by volume Î 如果固状悬浊物总量大于2-5%, 使用开放流道型防止堵
塞
Availability of chosen membrane可选用相应的膜 usually available in only 1 or 2 devices通常只有一两种超滤装
MILLIPORE
4
Microporous (MF) TFF Devices 切向流微滤装置(MF)TFF
MILLIPORE
5
TFF Devices, Uses and Operating Conditions
切向流(微滤)装置,使用及操作条件
Microporous (MF) TFF Devices
− suspended (open) screen channel (viscous solutions) 悬浮 (开放型) 筛网 (粘性溶液)
Highest efficiency (highest flux at given
cross flow velocity in feed channel) – 高效率 (在固定的切向流速下表现最高的通透量)
MILLIPORE
16
Typical TFF Devices, Uses and Operating Conditions
典型切向流超滤装置，使用及操作条件
Ultrafiltration Devices超滤装置
ÎThermoplastic flat plate (Prostak-UF) 热塑性平板式 (Prostak-UF)
– Aseptic applications灭菌应用
MILLIPORE
6
Microporous (MF) TFF Devices – Prostak 切向流微滤装置 - Prostak
Permeate
透过
Retentate回流
, Uses and Operating Conditions
切向流微滤装置(MF)
ÎGlass Reinforced (PES) flat plate (Prostak)
改良聚醚砜外壳平板膜 (Prostak)
– Flat plate, open flow channel平板式,开放流道
– (SIP) Steamable in place(SIP) 在线蒸汽灭菌
典型切向流超滤装置，使用及操作条件
Applications for Pellicon UF cassettes: PELLICON UF 盒式超滤装置的应用: High-value, low-to-medium volume product concentration / diafiltration, where high recovery required at small final volumes 高价值、低产量产品的浓缩及透析, 浓缩后最终体积较小时要求 保证良好的蛋白收率 – Where compact systems needed due to floor space restrictions 由于可放置设备的空间有限，紧凑型的超滤设备为最佳选择。
置可选
Mechanical complexity, reliability and ease-of-use构造复杂,可 靠但使用简单
Simplicity of scale-up线形放大的简单性
Cost (device AND operating system)成本(设备本身及相应操
作系统)
MILLIPORE
18
Typical TFF Devices, Uses and
– Bacterial, yeast, mammalian cMeIlLlLsI细PO菌RE，酵母和哺乳动物细胞
10
Ultrafiltration (UF) TFF Devices 切向流超滤装置（TFF）
MILLIPORE
11
Typical TFF Devices, Uses and Operating Conditions
TFF Devices切向流过滤装置
Basic Concepts of Tangential Flow Filtration (TFF) 切向流过滤的基本概组件(TFF)
MILLIPORE
1
Outline概要
Types of TFF Devices切向流过滤装置的种类 Îopen channel开放型流道 Îturbulence-promoted channels湍流增强型流道 Î"enhanced" by secondary flow次流道加强型
MILLIPORE
9
TFF Devices, Uses and Operating Conditions
切向流装置,使用及操作条件
Microporous TFF Devices切向流微滤
装置
ÎHollow fibers中空纤维
– Open flow channel (diameters vary)开放流道 (孔的直径大小各异)
典型切向流超滤装置，使用及操作条件
Prostack UF 的应用:
– High-value, medium-to-high volume product 高价值、高产量的产品
– Solvent-containing streams含溶媒的溶液
– Where cassette filter costs become too high 当盒式超滤膜成本太高时
Spiral-wound (screen
between flat sheets wound in
a spiral pattern)
卷式膜(卷起的膜每层之间有筛网)
MILLIPORE
3
TFF Device Selection-Considerations 超滤装置的选择-
从全面考虑的角度进行
Suspended solids in feed含悬浊的固状物进料
Maximum suspended solids
<5% to avoid plugging 最大悬浮固状物的量<5% 避免堵塞孔道
MILLIPORE
8
Microporous (MF) TFF Devices – Pellicon
切向流微滤装置 - Pellicon
Feed进口
Permeate透过
Retentate回流
Where very fast, easy scale-up needed 当要求快速、简单地实现线形放大时
MILLIPORE
13
Millipore提供从研发到生产规模的全 套超滤装置
搅拌式超滤杯 Labscale
Cogent M
离心式超滤管
Cogent SV
Lab
Pilot
Production
MILLIPORE
Stacks of Pellicon 2
20 cm 10 cm each way
cassette - from 0.5
from center to 80m² per holder
MILLIPORE
15
Millipore超滤系统真正实现线性放大
0.1m²
800x
80m²
Pellicon
Cassettes
from center
20 cm 10 cm each way
from center
Pellicon XL50 - 0.05 m²
Pellicon 2 “Mini” - 0.1 m²
20 cm 10 cm each way
from center
Pellicon 2 cassette - 0.5 m²
Open channel 开放流道
Flat plates (space between
membranes)平板 (膜中间有空
隙)
Hollow fibers and
tubes中空纤维及管 Turbulence-promoted channel 湍流增强型流道
Screen between flat
sheets/plates每层平板之间有筛网
– Limited burst pressures (25-50 psi)可承受的
压力有限 (25-50 psi)
– Steamability varies蒸汽灭菌性不稳定
– Minimum volumes vary widely 最小工作体积变化大
ÎApplications:应用:
– Cell harvest and clarification细胞收集和澄清
切向流(微滤)装置,使用及操作条件
Microporous TFF Devices
切向流微滤装置
Î Cassettes (Pellicon)盒式膜包 (Pellicon) – Flat sheet, 20-mesh screened channel平板式,20-MESH筛网 – Not steamable不能蒸汽灭菌
14
线性放大
All flow paths are identical in length, width, height and spacer & are fed in parallel.
Legend
Feed Flow Path Permeat Flow Path
20 cm 10 cm each way
– Minimum volumes > 5 L
– 最小工作体积 > 5 L
Î Applications:应用
– Cell harvest and clarification细胞收集和澄清
– Bacterial, yeast, mammalian cells细菌,酵母,
哺乳动物细胞
– Low shear applications低剪切力应用
– Lower minimum volumes than Prostak 比PROSTAK膜的残留体积更小
Î Applications:应用:
Clarification or bacterial cell harvest 澄清或细菌,细胞收集 - When minimum volumes
must be 100 mL-5 L 处理量范围 100 mL-5 L
Millipore's devices and their performance密理博公司 TFF装置及其性能
Recommended operating conditions for Millipore's TFF devices密理博公司超滤装置的建议操作条件
MILLIPORE
2
Types of TFF Devices TFF装置的类型