常用酶切位点表含保护碱基
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CGG GGTACC CCG
>90
>90
Mlul
GACGCGT C
0
0
CG ACGCGT CG
25
50
Ncol
CCCATGG G
0
0
CATG CCATGG CATG
50
75
Ndel
CCATATG G
0
0
CC CATATG GG
0
0
CGC CATATG GCG
0
0
GGGTTT CATATG AAACCC
75
>90
GGA AGATCT TCC
25
>90
BssHII
GGCGCGC C
0
0
AG GCGCGC CT
0
0
TTG GCGCGC CAA
50
>90
BstEII
G GGT(A/T)ACC C
0
10
BstXI
AACTGCAGAA CCAATGCATTGG
0
0
AAAACTGCAG CCAATGCATTGG AA
0
0
GGAATTC CATATG GAATTCC
75
>90
GGGAATTC CATATG GAATTCCC
75
>90
Nhel
G GCTAGC C
0
0
CG GCTAGC CG
10
25
CTA GCTAGC TAG
10
50
切割率%
酶
寡核苷酸序列
2hr
20hr
Notl
TTGCGGCCGC AA
0
0
ATTT GCGGCCGC TTTA
10
10
AA CTGCAG AACCAATGCATTGG
>90
>90
AAAA CTGCAG CCAATGCATTGGAA
>90
>90
CTGCAG AACCAATGCATTGGATGCAT
0
0
Pvul
CCGATCG G
0
0
AT CGATCG AT
10
25
TCG CGATCG CGA
0
10
Sacl
CGAGCTC G
酶
寡核苷酸序列
切割率%
2hr
20hr
Accl
G GTCGAC C
0
0
CG GTCGAC CG
0
0
CCG GTCGAC CGG
0
0
AflIII
CACATGT G
0
0
CC ACATGT GG
>90
>90
CCC ACATGT GGG
>90
>90
AscI
GGCGCGCC
>90
>90
AGGCGCGCC T
>90
GAGTACT C
10
25
AAAAGTACT TTT
75
75
Smal
CCCGGG
0
10
CCCCGGG G
0
10
CC CCCGGG GG
10
50
TCC CCCGGG GGA
>90
>90
Spel
GACTAGT C
10
>90
GGACTAGT CC
10
>90
CGG ACTAGT CCG
0
50
CTAG ACTAGT CTAG
>90
>90
Haelll
GG GGCC CC
>90
>90
AGC GGCC GCT
>90
>90
TTGC GGCC GCAA
>90
>90
Hindlll
CAAGCTT G
0
0
CC AAGCTT GG
0
0
CCC AAGCTT GGG
10
75
Kpnl
G GGTACC C
0
0
GG GGTACC CC
>90
>90
10
10
Sacll
GCCGCGG C
0
0
TCC CCGCGG GGA
50
>90
Sall
GTCGAC GTCAAAAGGCCATAGCGGCCGC
0
0
GC GTCGAC GTCTTGGCCATAGCGGCCGCG
10
50
G
10
75
ACGC GTCGAC GTCGGCCATAGCGGCCGCG
GAA
Seal
TTAATTAA
0
0
GTTAATTAA C
0
25
CC TTAATTAA GG
0
>90
Pmel
GTTTAAAC
0
0
G GTTTAAAC C
0
25
GG GTTTAAAC CC
0
50
AGCTTT GTTTAAAC GGCGCGCCGG
75
>90
Pstl
GCTGCAG C
0
0
TGCA CTGCAG TGCA
>90
TTGGCGCGCC AA
>90
>90
Aval
CCCCGGG G
50
>90
CCCCCGGG GG
>90
>90
TCC CCCGGG GGA
>90
>90
BamHI
CGGATCC G
10
25
CG GGATCC CG
>90
>90
CGC GGATCC GCG
>90
>90
Bglll
CAGATCT G
0
0
GA AGATCT TC
10
10
AAATAT GCGGCCGC TATAAA
10
10
ATAAGAAT GCGGCCGC TAAACTAT
25
90
AAGGAAAAAA GCGGCCGC AAAAGGAAAA
25
>90
Nsil
TGC ATGCAT GCA
10
>90
CCA ATGCAT TGGTTCTGCAGTT
>90
>90
Pacl
0பைடு நூலகம்
50
Sphl
GGCATGC C
0
0
CAT GCATGC ATG
0
25
ACAT GCATGC ATGT
10
50
Stul
AAGGCCT T
>90
>90
GAAGGCCT TC
>90
>90
AAAAGGCCT TTT
>90
>90
Xbal
CTCTAGA G
0
0
GCTCTAGA GC
>90
>90
TGC TCTAGA GCA
25
50
CTGCAGAA CCAATGCATTGG ATGCAT
25
>90
Clal
CATCGAT G
0
0
GATCGAT C
0
0
CC ATCGAT GG
>90
>90
CCC ATCGAT GGG
50
50
EcoRI
G GAATTC C
>90
>90
CG GAATTC CG
>90
>90
CCG GAATTC CGG
75
>90
CTAG TCTAGA CTAG
75
>90
Xhol
CCTCGAG G
0
0
CCCTCGAG GG
10
25
CCG CTCGAG CGG
10
75
Xmal
CCCCGGG G
0
0
CC CCCGGG GG
25
75
CCC CCCGGG GGG
50
>90
TCCC CCCGGG GGGA
>90
>90
>90
>90
Mlul
GACGCGT C
0
0
CG ACGCGT CG
25
50
Ncol
CCCATGG G
0
0
CATG CCATGG CATG
50
75
Ndel
CCATATG G
0
0
CC CATATG GG
0
0
CGC CATATG GCG
0
0
GGGTTT CATATG AAACCC
75
>90
GGA AGATCT TCC
25
>90
BssHII
GGCGCGC C
0
0
AG GCGCGC CT
0
0
TTG GCGCGC CAA
50
>90
BstEII
G GGT(A/T)ACC C
0
10
BstXI
AACTGCAGAA CCAATGCATTGG
0
0
AAAACTGCAG CCAATGCATTGG AA
0
0
GGAATTC CATATG GAATTCC
75
>90
GGGAATTC CATATG GAATTCCC
75
>90
Nhel
G GCTAGC C
0
0
CG GCTAGC CG
10
25
CTA GCTAGC TAG
10
50
切割率%
酶
寡核苷酸序列
2hr
20hr
Notl
TTGCGGCCGC AA
0
0
ATTT GCGGCCGC TTTA
10
10
AA CTGCAG AACCAATGCATTGG
>90
>90
AAAA CTGCAG CCAATGCATTGGAA
>90
>90
CTGCAG AACCAATGCATTGGATGCAT
0
0
Pvul
CCGATCG G
0
0
AT CGATCG AT
10
25
TCG CGATCG CGA
0
10
Sacl
CGAGCTC G
酶
寡核苷酸序列
切割率%
2hr
20hr
Accl
G GTCGAC C
0
0
CG GTCGAC CG
0
0
CCG GTCGAC CGG
0
0
AflIII
CACATGT G
0
0
CC ACATGT GG
>90
>90
CCC ACATGT GGG
>90
>90
AscI
GGCGCGCC
>90
>90
AGGCGCGCC T
>90
GAGTACT C
10
25
AAAAGTACT TTT
75
75
Smal
CCCGGG
0
10
CCCCGGG G
0
10
CC CCCGGG GG
10
50
TCC CCCGGG GGA
>90
>90
Spel
GACTAGT C
10
>90
GGACTAGT CC
10
>90
CGG ACTAGT CCG
0
50
CTAG ACTAGT CTAG
>90
>90
Haelll
GG GGCC CC
>90
>90
AGC GGCC GCT
>90
>90
TTGC GGCC GCAA
>90
>90
Hindlll
CAAGCTT G
0
0
CC AAGCTT GG
0
0
CCC AAGCTT GGG
10
75
Kpnl
G GGTACC C
0
0
GG GGTACC CC
>90
>90
10
10
Sacll
GCCGCGG C
0
0
TCC CCGCGG GGA
50
>90
Sall
GTCGAC GTCAAAAGGCCATAGCGGCCGC
0
0
GC GTCGAC GTCTTGGCCATAGCGGCCGCG
10
50
G
10
75
ACGC GTCGAC GTCGGCCATAGCGGCCGCG
GAA
Seal
TTAATTAA
0
0
GTTAATTAA C
0
25
CC TTAATTAA GG
0
>90
Pmel
GTTTAAAC
0
0
G GTTTAAAC C
0
25
GG GTTTAAAC CC
0
50
AGCTTT GTTTAAAC GGCGCGCCGG
75
>90
Pstl
GCTGCAG C
0
0
TGCA CTGCAG TGCA
>90
TTGGCGCGCC AA
>90
>90
Aval
CCCCGGG G
50
>90
CCCCCGGG GG
>90
>90
TCC CCCGGG GGA
>90
>90
BamHI
CGGATCC G
10
25
CG GGATCC CG
>90
>90
CGC GGATCC GCG
>90
>90
Bglll
CAGATCT G
0
0
GA AGATCT TC
10
10
AAATAT GCGGCCGC TATAAA
10
10
ATAAGAAT GCGGCCGC TAAACTAT
25
90
AAGGAAAAAA GCGGCCGC AAAAGGAAAA
25
>90
Nsil
TGC ATGCAT GCA
10
>90
CCA ATGCAT TGGTTCTGCAGTT
>90
>90
Pacl
0பைடு நூலகம்
50
Sphl
GGCATGC C
0
0
CAT GCATGC ATG
0
25
ACAT GCATGC ATGT
10
50
Stul
AAGGCCT T
>90
>90
GAAGGCCT TC
>90
>90
AAAAGGCCT TTT
>90
>90
Xbal
CTCTAGA G
0
0
GCTCTAGA GC
>90
>90
TGC TCTAGA GCA
25
50
CTGCAGAA CCAATGCATTGG ATGCAT
25
>90
Clal
CATCGAT G
0
0
GATCGAT C
0
0
CC ATCGAT GG
>90
>90
CCC ATCGAT GGG
50
50
EcoRI
G GAATTC C
>90
>90
CG GAATTC CG
>90
>90
CCG GAATTC CGG
75
>90
CTAG TCTAGA CTAG
75
>90
Xhol
CCTCGAG G
0
0
CCCTCGAG GG
10
25
CCG CTCGAG CGG
10
75
Xmal
CCCCGGG G
0
0
CC CCCGGG GG
25
75
CCC CCCGGG GGG
50
>90
TCCC CCCGGG GGGA
>90
>90