冠心舒通胶囊对高脂饮食ApoE- -小鼠动脉粥样硬化形成的影响-孙玉婵

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第22卷 第12期 2020 年 12 月

辽宁中医药大学学报

JOURNAL OF LIAONING UNIVERSITY OF TCM

Vol. 22 No. 12 Dec .，

2020

冠心舒通胶囊对高脂饮食ApoE-/-小鼠动脉粥样硬化形成的影响

孙玉婵1，张丹丹1，2

，路迎冬1，蒋之林1，林虹辰1，崔向宁1

（1.中国中医科学院广安门医院，北京 100053；2.北京市大兴区黄村医院，北京 102613）

基金项目：国家自然科学基金面上项目（81973842）

作者简介：孙玉婵（1992-），女，河北衡水人，硕士，研究方向：中西医结合防治心血管疾病。通讯作者：崔向宁（1969-），女，山东烟台人，主任医师、副教授，博士，研究方向：中西医结合防治心血管疾病。

摘要：目的 研究冠心舒通胶囊对高脂饮食诱导的ApoE 基因敲除（ApoE-/-）小鼠动脉粥样硬化形成的影响及

相关作用机制。方法 ApoE-/-小鼠高脂饲料喂养12周建立动脉粥样硬化模型，造模成功后随机分为3组：模型组（Model）、冠心舒通组（GXST）和阿托伐他汀组（Atorvastatin），同时以C57BL/6小鼠作为对照组（Sham），冠心舒通组和阿托伐他汀组分别灌胃给药8周，模型组和对照组给予生理盐水灌胃8周。采用酶联免疫吸附测定法（ELISA）检测血清中总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）等脂质代谢指标及丙二醛（MDA）、谷胱甘肽（GSH）和超氧化物歧化酶（SOD）等氧化应激指标；使用油红O 染色法观察小鼠主动脉斑块面积比值。结果 与对照组相比，模型组TC、TG、LDL-C 水平和MDA 含量明显升高（P <0.01），HDL-C、SOD 水平明显降低（P <0.01），GSH 水平下降（P <0.05），斑块面积比值、胶原纤维面积比值和CD68蛋白表达明显增加（P <0.01）；与模型组相比，冠心舒通组和阿托伐他汀组TC、TG、LDL-C 水平和MDA 含量均明显降低（P <0.01），HDL-C、SOD、GSH 水平均明显升高（P <0.01），斑块面积比值和胶原纤维面积比值明显下降（P <0.01），CD68蛋白表达降低（P <0.05）。结论 冠心舒通胶囊能够抑制高脂饮食诱导的ApoE-/-小鼠动脉粥样硬化的形成，其机制可能与改善血脂代谢及抑制氧化应激反应有关。

关键词：冠心舒通胶囊；动脉粥样硬化；血脂；氧化应激；斑块面积

中图分类号：R743 文献标志码：A 文章编号：1673-842X (2020) 12- 0028- 05

Effect of Guanxin Shutong Capsule on Atherosclerosis in ApoE-/-Mice with High-fat Diet

SUN Yuchan 1，ZHANG Dandan 1，2

，LU Yingdong 1，JIANG Zhilin 1，LIN Hongchen 1，CUI Xiangning 1

（1.Guang'anmen Hospital，China Academy of Chinese Medical Sciences，Beijing 100053，

China；2.Beijing Huangcun Hospital of Daxing District，Beijing 102613，China）

Abstract：Objective To study the effect and correlation mechanism of Guanxin Shutong Capsule on

atherosclerosis in apolipoprotein E knockout（ApoE-/-）mice with high-fat diet. Methods ApoE-/- mice were fed high-fat diet for 12 weeks to establish the animal model of atherosclerosis. Then，these mice were randomly divided into 3 groups：a model group（model），Guanxin Shutong group（GXST），and atorvastatin group（atorvastatin），and C57BL/6 mice were treated with routine diets as the sham group（sham）.The GXST group and atorvastatin group were given the corresponding treatment by gavage respectively for 8 weeks. And the control group and model group were given normal saline gavage for 8 weeks. After 8 weeks of treatment，serum lipid metabolic indexes such as total cholesterol（TC），triglyceride（TG），low density lipoprotein cholesterol（LDL-C），high density lipoprotein cholesterol（HDL-C）and oxidative stress indexes such as malondialdehyde（MDA），glutathione（GSH）and superoxide dismutase（SOD）were detected by ELISA. The ratio of plaque area in the aortic of mice was observed by oil red O staining. Results Compared with the sham group，the levels of TC，TG，LDL-C and the content of MDA in model group were significantly increased（P <0.01），and the levels of HDL-C and SOD were significantly reduced （P <0.01），the level of GSH was decreased（P <0.05），the ratio of plaque area，the area ratio of collagen fibers and the expression of CD68 protein were significantly increased（P <0.01）. Compared with the model group，the levels of TC，TG，LDL-C and the content of MDA in GXST and atorvastatin group were significantly reduced （P <0.01），the levels of HDL-C，SOD and GSH were significantly increased （P <0.01），the ratio of plaque area and the area ratio of collagen fibers were significantly decreased（P <0.01），and the expression of CD68 protein was lower（P <0.05）. Conclusion Guanxin Shutong Capsule can inhibit the formation of atherosclerosis induced by high fat diet in ApoE-/- mice，and its mechanism may be related to improving lipid metabolism and inhibiting the reflection of oxidative stress.

Keywords：Guanxin Shutong Capsule；atherosclerosis；blood lipid；oxidative stress；plaque area

DOI：10.13194/j.issn.1673-842x.2020.12.007