ELISA标准曲线制作方法.pdf

ELISA标准曲线制作方法

一般而言，我们拟合ELISA标准曲线选用比较经典的Curve Expert 1.3或者Curve Expert 1.4软件。现在我们以Curve Expert 1.4为例，对ELISA标准曲线绘制方法进行详述。

Generally speaking, the standard fitting curve of ELISA is based on the classic software

of Curve Expert 1.3 or Curve Expert 1.4. Now we will use the Curve Expert 1.4 to

illustrate how to construct the ELISA standard curve.

1.点击Curve Expert 1.4，打开应用程序，截图界面如下：

Click Curve Expert 1.4, and then open the application programs. You will see the

following screen shot.

2.在X轴输入标准品的OD值，Y轴输入相应的标准品的浓度，截图界面如下：

Input the value of OD on the X-axis against the concentration of samples on the Y-axis. The following screen shot will appear like this.