微生物基因组denovo测序分析流程

#流程大放送#微生物基因组Denovo测序分析

知因无限

一介绍

微生物基因组De novo测序分析也叫微生物基因组从头测序分析，指不依赖于任何参考序列信息就可对某个微生物进行分析的测序分析技术，用生物信息学的方法进行序列拼接获得该物种的基因组序列图谱，然后进行注释等后续一系列的分析。微生物Denovo基因组测序及分析技术可以应用于医药卫生等领域。

二技术应用领域

1、基因组图谱的系统性构建

例子：过去几个月，肠病毒D68令数百名美国儿童患病。华盛顿大学的研究人员测序和分析了肠病毒D68（EV-D68）的基因组，这一成果将发表在新一期的Emerging Infectious Diseases杂志上。（Genome Sequence of Enterovirus D68 from St. Louis, Missouri, USA）肠病毒D68（EV-D68）能在儿童中引起严重的呼吸道疾病。其基因组序列可以“帮助人们开发更好的诊断测试，”共同作者Gregory Storch说。“有助于解释病毒感染为什么会造成严重的疾病，以及EV-D68为什么比过去传播得更广。”（来自于生物通的报道）

2、微生物致病性和耐药性位点检测及相关基因功能研究

例子：根据分泌蛋白、毒力因子、致病岛、必需基因等结果去探讨所测物种致病性和耐药性。

3、微生物的比较基因组分析，确定各个近缘微生物中的系统发育关系

二基本分析流程图

三可能的结果展示图

示例图1 微生物基因组的功能注释

示例图2 微生物基因组的系统进化关系

注：以上图片和文字来自参考文献21。

六参考文献

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