水培系统中纳米银颗粒对湿地植物香蒲抗逆特性的影响
- 1、下载文档前请自行甄别文档内容的完整性,平台不提供额外的编辑、内容补充、找答案等附加服务。
- 2、"仅部分预览"的文档,不可在线预览部分如存在完整性等问题,可反馈申请退款(可完整预览的文档不适用该条件!)。
- 3、如文档侵犯您的权益,请联系客服反馈,我们会尽快为您处理(人工客服工作时间:9:00-18:30)。
JournalofSoutheastUniversity EnglishEdition ㊀Vol.35 No.3 pp.381388Sept.2019㊀ISSN1003 7985
EffectsofsilvernanoparticlesonresistancecharacteristicsofthewetlandplantTyphaorientalisinahydroponicsystem
MaYixuan㊀
HuangJuan㊀
CaoChong㊀
CaiWenshu㊀
XiaoJun㊀
YanChunni
(SchoolofCivilEngineeringꎬSoutheastUniversityꎬNanjing210096ꎬChina)
Abstract:Toprobetheinfluenceandtheadverse ̄resistancecharacteristicsofwetlandplantsinpresenceofsilvernanoparticles(AgNPs)ꎬthechangesinthephysiologicalandbiochemicalcharacteristics(includingthesuperoxidedismutase(SOD)activityꎬcatalase(CAT)activityꎬperoxidase(POD)activityꎬsolubleproteincontentꎬandchlorophyllcontent)ofTyphaorientalisexposedtodifferentconcentrationsofAgNPssolutions(0ꎬ0.1ꎬ1ꎬ20and40mg/L)wereexplored.Meantimeꎬtheaccumulationofsilvercontentintheseplantswasrevealed.TheresultsshowthatunderlowconcentrationsofAgNPsꎬtheSODandPODactivitiesintheleavesofTyphaorientalisarestrengthenedtodifferentdegrees.HoweverꎬhighconcentrationsofAgNPsinhibittheactivitiesofSODandPOD.UnderthestressofdifferentconcentrationsofAgNPsꎬtheCATactivitiesareinhibitedinitiallyandlaterrecoveredtosomeextent.UnderthestressoflowconcentrationsofAgNPsꎬthesolubleproteincontentintheleavesofTyphaorientalisincreasessignificantlyꎬbutdecreasesmoresignificantlywithincreasingconcentrationsofAgNPs.LowconcentrationsofAgNPspromotechlorophyllsynthesisintheleavesofTyphaorientalisꎬbutthechlorophyllcontentsubsequentlyfallstopre ̄stresslevels.IncontrastꎬhighconcentrationsofAgNPscauseacertaininhibitiontogeneratechlorophyll.MeanwhileꎬtheresultsshowthatthesilverconcentrationsofplanttissuesincreasewiththeexposureofconcentrationsofAgNPsandtheyhaveapositiverelationshipwiththeexposureofconcentrationsofAgNPs.
Keywords:silvernanoparticles(AgNPs)ꎻwetlandplantsꎻTyphaorientalisꎻphysiologicalandbiochemicalcharacteristicsꎻantioxidase
DOI:10.3969/j.issn.1003-7985.2019.03.015
Received2018 ̄06 ̄03ꎬRevised2019 ̄08 ̄12.
Biographies:MaYixuan(1995 )ꎬmaleꎬPh.D.candidateꎻHuangJuan(correspondingauthor)ꎬfemaleꎬdoctorꎬassociateprofessorꎬseu070703@126.com.
Foundationitem:TheNationalNaturalScienceFoundationofChina(No.51479034ꎬ5151101102).
Citation:MaYixuanꎬHuangJuanꎬCaoChongꎬetal.EffectsofsilvernanoparticlesonresistancecharacteristicsofthewetlandplantTyphaori ̄entalisinahydroponicsystem[J].JournalofSoutheastUniversity(EnglishEdition)ꎬ2019ꎬ35(3):381388.DOI:10.3969/j.issn.1003-7985.2019.03.015.
S
ilvernanoparticles(AgNPs)havebecomeoneofthemostwidelyusedandquicklydevelopednanomateri ̄alsduetotheirhighsurfactivityꎬsurfaceenergyandcata ̄
lyticperformance.Globallyꎬthetotalmassofthecon ̄sumerproductscontainingsilvernanoparticlesisapproxi ̄mately320teveryyear[1]ꎬwhichcanbeusedinawiderangeofapplicationsꎬfromconsumerproducts(clothingꎬtextilesandhygieneproductsꎬetc.)todisinfectingmedi ̄caldevicesꎬhomeappliancesꎬandwatertreatment[2].Withtheincreasinguseofnanosilverproductsꎬthepossi ̄bilityofAgNPsreleasedintotheenvironmentisalsoin ̄creasing.PreviousresearchhasindicatedthatAgNPsmayentertheenvironmentthroughsewagedischargeandsur ̄facerunoff[34].Asaconventionalwastewatertreatmentecologicalsystemꎬtheconstructedwetlands(CWs)arealsofacingAgNPspollution[5].InadditionꎬthewetlandplantsareoneofthemostessentialcomponentsinCWsꎬwhichhaveseveralpropertiesrelatedtothetreatmentprocess[6].Manystudiesshowedthatwetlandplantscanpromotetheremovalefficiencyofpollutantssuchaschemicaloxygendemand(COD)ꎬtotalnitrogen(TN)andtotalphosphorus(TP)[7].AgNPsareoneofthemosttoxicmetallicnanoparticlestoaquaticorgan ̄isms[89].Baoetal.[10]foundthatAgNPscanenterplanttissuesandtendtoaccumulatepredominantlyintheap ̄oplastofroottissueswherebyaminorportionwastrans ̄portedtoshoottissues.HenceꎬitisnecessarytoevaluatetheriskoftoxiceffectsofAgNPsonthewetlandplants.㊀TheeffectsofAgNPshavebeenwidelystudiedinmanyaspectsꎬsuchasmicroorganismsꎬmammaliancellsandplants[1113].Inthetermsofplantsꎬtherewassomeresearchaboutthetoxiceffectsoncropsꎬvegetablesandforagegrassꎬetc.[1216]andtheexposureofAgNPscancausethegenedamageꎬlowergerminationofseedsꎬthedecreaseofrootelongationandreductionofbio ̄mass[1112ꎬ17].El ̄TemsahandJoner[18]treatedryegrassseedwith10mg/LAgNPsandobserveda20%decreaseintheirgermination.MeantimeꎬotherstudiesfoundthatAgNPsaffectedthephysiologicalandbiochemicalindicesofplantssuchassuperoxidedismutase(SOD)activityꎬcatalase(CAT)activityꎬperoxidase(POD)activityꎬsolubleproteincontentandchlorophyllcontent.Whentheplantssufferedthestressꎬsuchasfromheavymetalsꎬtheplant scellaccumulatedreactiveoxygenspecies(ROS)suchashydroxylradicals(OH )andhydrogenperoxide(H2O2).Itcanleadtotheincreaseinlipidperoxidationandproteinꎬresultinginbiologicalmacromolecule(i.e.nucleicacidandenzyme)damageꎬwhichseriouslyinter ̄