引物设计及合成常识及一般问题

其中红色区域为CDS序列

Rabbit IL-4:

AF169170.1

1 ccactgcact attg atgggg ctccctgccc agctgcctgt cactctgctc tgcctcctcg

61 caggcaccgc gcacttcatc caggggcgca ggggcgacat catcctaccc gaagtcatca

121 aaaccctgaa catcctcaca gagagaaaga acacaaccga gagagaggcc gtctgcaggg 181 ccgcgactgc actccggcag ttctacctcc accacaaggt gtcttggtgc ttcaaggagc

241 acggagagct cggtgacctc agactcctga gaggactcga caggaacctc tgcagcatgg

301 cgaagctgag caactgtcct gggaaggaag ccagacagac tacactggaa gacttcttag

361 acaggctaaa gaccgccatg caagagaaat actcaaagcg tcagagctga

Rabbit IL-10:

GenBank: AF068058.1

1 aaagcaaacc acaaggcgga ctcgtagaag caggcagagt tccacc atgc tcagctcagc

61 tctgctatgt tgcctggtct tcctgggtgg gacaggggcc agccgaggcc aggacacccc

121 tgctgagaac agctgcattc actttccagg cggcctgccc cacatgctcc gcgagctccg

181 tgctgccttt ggcagggtga agactttctt tcaatcgaag gatcagctga acagcatgtt

241 gttaaccgag tccctgctgg aggaccttaa gggttacctg ggatgccaag ccttgtcgga

301 gatgatccag ttttacctga aggacgtgat gccgcaagct gagaaccaca gtccagccat

361 cagggagcac gtgaactccc tgggggaaaa cctgaagacc ctcaggctga ggctgcgaca

421 atgtcaccga tttctcccct gtgaaaacaa gagcaaggca gtggagcagg tgaagagcgc

481 cttcagcaag ctgcaagagg aaggcgtcta caaagccatg agtgagtttg acatcttcat

541 caactacata gaaacctaca tgacaatgaa gataaaaagc taaa agcccc aggatggcaa

601 ctcggctaga gtctaggaca tcagttaggg acctgcacac cctgggtcag ctgacccagc

661 accttggaaa gctgttgtac ctctcaatat ttattacctc tgatacctca gctcccgatc

721 ctatttattt accgagcttc tctgtgaact ctttagaaag aagcccacta ttataatttt

781 ttcagtattt attattttca cctgcattta agctgtaccc atggggtgat gccctgtggg

841 atttgagtgt cttaggagaa attataattt atgtgaaagg gaaaatgtgc cttggggagc

901 cgactgaggc ttccattcct tctgtgcctg accacacttt ctaactccta agccgagctc

961 cctcttaccc tctggagccc ggacctgggt ctcgagtgtt ccagagactc ctagcctctt

1021 aggaagagag accggaagcc cttgggtggt gaccttccgg cagctcagag ggaggctcct 1081 gacctcgat

Rabbit TNF-α :

GenBank: M12845.1

1 aagctccctc agtgaggaca cgggcaccag ctaggaggga gagcagcaac tccaggctac

61 cccggcagta actcccagac gccgccccgc cagacaagct gccagccggt gtgccgtctt

121 cctctctcac gcaccagccc ccggttccac cacctccctc tggagagagc gcc atgagca

181 ctgagagtat gatccgggac gtcgagctgg cggaggggcc gctccccaag aaggcagggg 241 ggccccaggg ctccaagcgc tgcctctgcc tcagcctctt ctctttcctg ctcgtggctg

301 gagccaccac gctcttctgc ctgctgcact tcagggtgat cggccctcag gaggaagagt

361 ccccaaacaa cctccatcta gtcaaccctg tggcccagat ggtcaccctc agatcagctt

421 ctcgggccct gagtgacaag cctctagccc acgtagtagc aaacccgcaa gtggagggcc

481 agctccagtg gctgagccag cgtgcgaacg ccctgctggc caacggcatg aagctcacgg