Bioinformatics生信案例
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精子生成障碍病例及其对照人群
基因组DNA甲基化谱分析
Roche-NimbleGen HG18 Meth 3×720K CpG plus Promoter
DNA甲基化模式异常基因的筛选 人群验证
异常DNA甲基化模式基因与EDCs 交互作用分析
Genetics
Biochemistry
Bioinformatics
Molecular biology
Computer science
molecular biology genetics biochemistry other biological science computer science mathematics statistics ……
Bioinformatics
What is Bioinformatics?
Bioinformatics is a new subject of genetic data collection, analysis and dissemination to the research community Dr. Hwa A. Lim，1987
Application in bioinformatics
or Case Control
Case
Control
Thousands of genes, Thousands of problems, while Thousands of papers
1
2 Gene annotation
2 Microarray presentation
A very brief history of bioinformatics:
1965 1970 1974 ~1979 Margaret Dayhoff and collaborators at National Biomedical Research Foundation develop first protein sequence database Saul Needleman and Christian Wunsch develop first sequence alignment algorithm Chou and Fasman develop first protein structure prediction algorithm GenBank established as a nucleic acid sequence database at Los Alamos National Laboratory
Epigenetic involved with TP53?
Methylation for example, first we need to detect the CpG island in promotor region
CpG island is obtained
四、下一步研究计划
3
18
精子生成障碍的全基因组DNA甲基化谱分析
Bioinformatics involves the technology that uses computers for storage, retrieval, manipulation, and distribution of information related to biological macromolecules such as DNA, RNA, and proteins Dr. Jin Xiong, Essential Bioinformatics
1987
1988 1990
mid-90s
Perl computer programming language released by Larry Wall
National Center for Biotechnology Information (NCBI) is established at NIH/NLM Stephen Altschul develops BLAST for searching sequence databases The term “bioinformatics” becoming widely used First bacterial genomes sequenced
Cluster analysis
Pathway analysis
Detailed analysis by softwear
We have selected the P53 gene as a candidate gene for our search
Location of the p53
miRNA regulated with p53
Does TP53 could interacted with transcription factors? Find the promotor of TP53
Obtain the DNA sequence of TP53
Change the site by plus 2000bp to get the promotor sequence
1995 2001
Human genome sequence is published, Dataved in bioinformatics
What can we obtain from Bioinformatics
• • • • • • Research design Microarray analysis Structure description Sequence searching Subcellular location Proteomics
基因组DNA甲基化谱分析
Roche-NimbleGen HG18 Meth 3×720K CpG plus Promoter
DNA甲基化模式异常基因的筛选 人群验证
异常DNA甲基化模式基因与EDCs 交互作用分析
Genetics
Biochemistry
Bioinformatics
Molecular biology
Computer science
molecular biology genetics biochemistry other biological science computer science mathematics statistics ……
Bioinformatics
What is Bioinformatics?
Bioinformatics is a new subject of genetic data collection, analysis and dissemination to the research community Dr. Hwa A. Lim，1987
Application in bioinformatics
or Case Control
Case
Control
Thousands of genes, Thousands of problems, while Thousands of papers
1
2 Gene annotation
2 Microarray presentation
A very brief history of bioinformatics:
1965 1970 1974 ~1979 Margaret Dayhoff and collaborators at National Biomedical Research Foundation develop first protein sequence database Saul Needleman and Christian Wunsch develop first sequence alignment algorithm Chou and Fasman develop first protein structure prediction algorithm GenBank established as a nucleic acid sequence database at Los Alamos National Laboratory
Epigenetic involved with TP53?
Methylation for example, first we need to detect the CpG island in promotor region
CpG island is obtained
四、下一步研究计划
3
18
精子生成障碍的全基因组DNA甲基化谱分析
Bioinformatics involves the technology that uses computers for storage, retrieval, manipulation, and distribution of information related to biological macromolecules such as DNA, RNA, and proteins Dr. Jin Xiong, Essential Bioinformatics
1987
1988 1990
mid-90s
Perl computer programming language released by Larry Wall
National Center for Biotechnology Information (NCBI) is established at NIH/NLM Stephen Altschul develops BLAST for searching sequence databases The term “bioinformatics” becoming widely used First bacterial genomes sequenced
Cluster analysis
Pathway analysis
Detailed analysis by softwear
We have selected the P53 gene as a candidate gene for our search
Location of the p53
miRNA regulated with p53
Does TP53 could interacted with transcription factors? Find the promotor of TP53
Obtain the DNA sequence of TP53
Change the site by plus 2000bp to get the promotor sequence
1995 2001
Human genome sequence is published, Dataved in bioinformatics
What can we obtain from Bioinformatics
• • • • • • Research design Microarray analysis Structure description Sequence searching Subcellular location Proteomics