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Rho-GEF homology domain
精选 Sprouty
Sprouty domain
PTB
PTB domain
PH
Plekstrin homology domain
Protein separation using SDS-PAGE
(Laemmli system)
1. Apply protein/dye samples into polyacrylamide gel wells
binding
STAT
binding
Jak
Jak
3
3
STAT
binding
STAT
binding
Jak 3
STAT
binding
STAT
binding
SHP SHP-binding
Ig Ig - like
Adaptor proteins - selected (human)
1
SH3
SH2
217
SH3
BLNK
SLP-76
SHC-1
APS
845
SH3
Vav-1
SH3
Src-homology 3 domain
SH2
Src-homology 2 domain
SAM
C-1
SAM domain
Cysteine-rich region 1
Calponin
Calponin homology domain
Rho-GEF
further utilization
Potential use for
therapeutic clonin精g 选
Use for In Vitro fertilization
Freeze in N2 for long-term storage
2. Run the electrophoresis until dye reaches the end of the gel
Stacking gel
Resolving gel
3. Remove the gel from the apparatus and stain for proteins
精选
A Column Chromatography Toolkit
III
FN
WS-WS
III FN
III
FN
III
FN
III
LIF-R 1053 aa
FN
CCCC
III FN
WS-WS
III
Ig
FN
CCCC
III
FN
WS-WS
III FN
III
FN
III
FN
III
Tyk Jak
2
1
STAT
binding
Jak Tyk
1
2
Jak Jak
Jak
1
2
1
Jak
Tyk
Jak
FN
IL-10R2 325 aa
III
FN FN
FN FN
III III
III III
FN FN
FN FN
III III
III III
INFR1 489 aa
IL-22R1 574 aa
INFR2 332 aa
FN FN
FN
III III
III
FN FN
FN
III III
III
IL-13R1 427 aa
IL-4R 825 aa
Ig
FN
CCCC
III
FN
WS-WS
III
Ig
FN
CCCC
III
FN
WS-WS
III
IL-13R2 380 aa
c 349 aa
Ig
FN
CCCC
III
FN
WS-WS
III
Ig
FN
CCCC
III
FN
WS-WS
III
gp130 896 aa
Ig
FN
CCCC
III FN
WS-WS
III FN III FN III FN III
1
276
SH3
SH2
1
SH2
SH3
304
SH3
1
SH3
SH2
330
SH3
1
SH3
SH3
SH3
377
SH2
1
Sprouty
456
SH2
1 SAM
533
SH2
1
PTB
584
SH2
1
632
PH
SH2
1 Calponin
Rho-GEF
PH
C-1
SH3
SH2
GRB2
SLAP
Crk
GADS (GRID)
Nck1
精选
Embryonic stem cells
Sperm donor Egg donor
Egg fertilization
Culture fertilized cells for several cell divisions
Dissociate cell cluster for subculturing and
OSM-R 952 aa
IL-12 662 aa
IL-6R 449 aa
IL-11R 400 aa
FN
CCCC
III
FN
WS-WS
III
FN
Ig Ig III
FN
CCCC
III
FN FN
CS WS-WS
III
III III
FN
WS-WS
III
Ig
FN
CCCC
Ca
Ca Ca Ca
Ca
Ca
Ca CaM
Ca Ca
Ca Ca Ca
Ca
MLCK
Ca
Ca
Ca
精选
Ca
CaM
Ca
P MLCK
P
myosin
myosin
P
myosin
RGS function and regulation
Ligand
S
GDP RGS
TNF
14-3-3 P
RGS
Ligand
S
GTP RGS
NO – mediated attenuation of adrenergic Gq signaling in VSMC
ANP
K+ BKCa
pGC
sGCCa
cGMP
PKG
PLC
q
GTP
q
GTP
PA
1,4,5-Ins
PKC
q
GDP
Ca Ca
Ca Ca Ca
Ca
Ca Ca Ca
Ca Ca Ca
Ca Ca Ca Ca
III with WSXWS
IRS IRS-binding SOCS SOCS-binding
STAT
binding
STAT
binding
STAT
binding
SHP
Jak
2
精选
STAT STAT-binding
Tyk
binding
2
JAK/TYK -binding
Jak 1
SOCS SHP
STAT
1
2
3
IRS
SOCS
STAT
binding
STAT
binding
FN Fibronectin III type III domain
FN Fibronectin
CCCC type III domain
III with cysteines
STAT
binding
FN Fibronectin
WS-WS type III domain
P
RGS
精选
S
GTP AC AC
cAMP cAMP
cAMP cAMP
cAMP
cAMP
cAMP
cAMP cAMP
cAMP
PKA
14-3-3
Modular Structure of Cytokine Receptors
IFNR1 530 aa
IL-10R1 557 aa
FN
III
IFNR2 515 aa