《生物实验技术》牡蛎组织切片示例

2. Each oyster is measured from the hinge to the bill in millimeters, following the curve of the shell.
3. Oystewk.baidu.coms are shucked from the hinge to avoid damaging delicate tissues.
13. Paraffin is removed from the slides with solvents. The tissues 14. A permanent protective glass coverslip is mounted over the are routinely stained with Mayer’s hematoxylin (purple) and stained section. eosin (orange).
4. The oyster rectum is excised to culture for Perkinus marinus, which causes dermo-disease.
固定
7. Cross sections are fixed, dehydrated, infiltrated with molten 8. Each tissue cross section is moved from its labeled cassette
paraffin, and embedded in blocks of paraffin.
into a mold filled with molten paraffin.
9. The labeled cassette is placed atop the mold and it is placed 10. Ribbons of 5-6 micron thick sections of the cross section are
on a chilling plate to solidify the paraffin block
cut from the paraffin block on a specialized instrument called a
microtome.
11. The ribbon is floated on a waterbath to spread the sections 12. Unstained paraffin sections of razor clam tissues drying. and individual sections are attached to glass slides.
牡蛎组织切片示例
1. There are 30 oyster in a standard sample. They are scrubbed free of fouling organisms, separated from one another, and layed out on a tray.
15. Finished microscope slides of oyster tissues stained with Mayer’s hematoxylin and eosin.