检验医学课件

Epo imcompensatory increase
This is found in kidney disease and cancer. such as liver cancer , renal carcinoma and ovarian cancer ,et al.
Primary erythrocytosis Polycythemia vera
First General examination of blood
Collection of specimen
•Venipuncture specimen: • Syringe Collection •Vacuum Collection tube
Anticoagulants
• The blood should be collected into a tube containing an anticoagulant and thoroughly mixed.
Etiologic of ຫໍສະໝຸດ Baidunemia
Decreased Production
–Aplastic anemia –Deficiency anemia Iron, B12, Folate etc. –Lack of erythropoiesis - Kidney disease –Marrow disease, malignancy, radiation
Erythrocytosis
Secondary erythrocytosis Primary erythrocytosis
Secondary erythrocytosis
Epo Epo Epo Epo
Epo compensatory increase Epo imcompensatory increase
Epo compensatory increase
Physiological increase Pathological increase
Physiological increase
Pathological increase
pulmonary heart disease
cyanotic congenital heart disease
• The choice of anticoagulants for hematologic studies:
EDTA
Blood cell counting
The process of performing a basic hematologic analysis of peripheral blood involves four primary steps:
RBC
4. 0~5. 5x1012/L 3. 5~5. 0x1012/L 6. 0~7. 0x1012/L
Hb
120~160g/L 110~150g/L 170~200g/L
Clinical significance of RBC and Hb
Increased RBC and Hb
Decreased RBC and Hb
Destruction of senile RBC
Mononuclear-macrophage system
RBC count and Hb detecting (emphasis)
Reference interval of RBC count and Hb
Adult male Adult female At birth
Increased RBC and Hb
Adult male : RBC>6.0x1012/L Hb>170g/L Adult female :RBC>5.5x1012/L Hb>160g/L
Relative increase
Absolute increase
Relative increase
Absolute increase
Epo Epo
Erythropoietin,EPO
Epo Epo
Feature and function of RBC
1.Number of red blood cells are more 2.RBC posses feature of deformability 3.RBC are used as respiratory carrier 4.RBC running in blood circulation about 150 km 5.erythrocyte life span is about 120 day 6.RBC are abundantly destroyed— Hemolysis Exceed marrow compensation ability—— Hemolytic anemia
RBC
PLT
WBC
Decreased RBC and Hb
physiological decrease
Pathological decrease
Anemia (emphasis)
“Anemia is decreased red cell
mass affecting tissue oxygenation”
1.collection and processing of the peripheral blood sample
2.determination of the CBC:RBC,WBC,PLT. 3.determination of the differential WBC 4.blood film examination
Department of Laboratory diagnosis
Huanglijuan
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Composition of blood
Anticoagulants Hematocyte
Plasma（55%） WBC、PLT
RBC
Second chapter Clinical hematology examination
Parameter of red blood cell
Parameter of red blood cell （RBC）
•including determination of RBC， Hemoglobin（Hb），Hematocrit （Ht）， MCV, MCHC, MCH and RDW
Erythropoiesis