大鼠肝损伤动物模型的建立

陈小伍;李利红;朱达坚;戎祯祥;剧永乐

【期刊名称】《中华实验外科杂志》

【年(卷),期】2009(026)012

【摘要】Objective To explore the methods of establishing the model of surgical hepatic lesions in rats. Methods 40 SD rats,distributed into 2 groups randomly,The model group (re=20) :to be resected 70% of the whole liver;the control group (n =20) ;to be opened and closed the peritoneal cavity under the same condition, the levels of ALT, AST, ALP,TB, DBIL of the both groups were assayed pre-op-eration and at 12 hours and days 1,3,5,7,14 after operation respectively. Results The levels of AST, ALP,DBIL were statistical significances in the model group within 12 h to 7 d and ALT,TB were statistical significances within 12 h to 5 d after operation compared with the control group (P<0.05). There were no statistical significances for all of the indicators between both groups at the 14th d. The histological slices of the liver section of the control group showed that it was loose among the hepatocytes, the edge of which were sharp,the hepatic sinusoid was wider, whereas the model group indicated that the hepatocyte were hydropic,hypertrophic.eosinophilic change. Conclusion To resect 70% volume of the whole liver of SD rats, which could establish the model of the surgical hepatic lesions effectively.%目的探讨制作SD大鼠肝损伤动物