微阵列数据分析工具PPT演示

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需要有一组微阵列实验数据的解释工具
1. GOstat 2. Panther 3. MILANO 4. Genomatix
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Prepare input data
• Gene identifiers 1. Gene symbols (e.g. icam3) 2. Gene description (e.g. mitogen-activated protein kinase 1) 3. Entrez gene identifier or GeneID (e.g. LOC5166 or 5166) 4. RefSeq ID (e.g. NM_02044) 5. ……
GO:0048513 organ development
TBX1 TLE2 CD1D DRD2 STRBP TNNT2 NRCAM MAP4K2 HLA-DMA VGF EGR1 ITPKB CTNNBIP1 ADAMTS9 SEMA6A FLI1 ELN NPAS2 MEOX1 TAGLN ANKRD2 NEB DCT EGR3 DKK2 CRIP2 KRT4 INHA
PEX6 DAPK2 CMTM5 PI3 TLE2 TLN2 LIN7B CFTR RARA NRCAM MAP4K2 HLA-DMA CTNNBIP1 SEMA6A RNF24 SIPA1L1 FMNL3 KCNB2 TAGLN ITFG1 KLHL22 MXD4 GAS6 GRM1 FGD3 ACCN2 ZNRF3 TBX1 MAML2 CD1D CNTN5 CLIC5 LMNA TNNT2 VGF MYO1F PHACTR1 MUC4 ITPKB CRLF1 HSPB9 ABLIM2 STRN AKAP8 TNNC1 ITPR3 TRIM29 GRB7 NEB CD84 WISP2 LOC148709 MBD1 JUP KRT4 LRRC27 ARRB1 INHA TBX1 TLE2 CD1D TNNT2 MAP4K2 HLA-DMA VGF EGR1 ITPKB SEMA6A FLI1 ELN ANKRD2 TAGLN NEB DCT EGR3 CRIP2 INHA
GO:0005886 plasma membrane
Genes
FGD3 TBX1 TAOK2 TLE2 CD1D DRD2 TNNT2 NRCAM MAP4K2 HLA-DMA VGF EGR1 ITPKB SEMA6A FLI1 ELN NPAS2 TAGLN ANKRD2 NEB DCT WISP2 EGR3 CRIP2 GAS6 KRT4 INHA
ACCN2 KIR2DL4 FXYD3 SLC2A5 TLN2 CD1D LIN7B DRD2 CFTR NRCAM HLA-DMA SLC2A9 MUC4 MC3R KCNB2 PSCA ITPR3 KCNQ1 SLC22A18 CD84 MMP24 CRHR2 ABCA4 WISP2 PRSS8 SLC6A17 JUP GRM1 ALOX15 ARRB1
……
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Benefits of Ontology Stats
TLE2 LIN7B CFTR NRCAM MAP4K2 HLA-DMA EGR1 ADAMTS9 CTNNBIP1 SEMA6A C2 FLI1 NPAS2 KCNB2 TAGLN KCNQ1 MYL9 SLC22A18 EGR3 GRM1 TBX1 CD1D DRD2 CLIC5 STRBP CNTN5 TNNT2 VGF ITPKB ELN TNNC1 MEOX1 ANKRD2 NEB DCT ABCA4 DKK2 CRIP2 KRT4 INHA ARRB1
TBX1 TLE2 CD1D DRD2 TNNT2 NRCAM MAP4K2 HLA-DMA VGF EGR1 ITPKB SEMA6A FLI1 NPAS2 ELN TAGLN ANKRD2 NEB DCT EGR3 CRIP2 INHA
KCNB2 TNNC1 MYL9 KCNQ1 CD1D TNNT2 HLA-DMA ITPKB GRM1 C2 INHA
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GOstat
• http://gostat.wehi.edu.au/ • GO数据库是注释和分析基因功能的有效工具.微阵列实验
往往会产生大量的基因列表.为了获得这类型数据的生物 学意义,我们需要找到这些数据的功能注释或是能代表它 们的GO分组. • GOstat程序就是用来分析这个的基因列表, 根据GO注释的 功能进行分类.
GO:0007275 multicellular organismal development
GO:0048731 system development
GO:0051239 regulation of multicellular organisma百度文库 process
GO:0005515 protein binding
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Best GOs (Max: 30)
GO:0048856 anatomical structure development
GO:0032501 multicellular organismal process
微阵列数据分析工具
2007-5-31 闫微
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Experiment vs Contro Log Ratiol
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Gene identifiers
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• 表达下调2倍以上的基因有3810个、8倍以上的基因有197 • 如何对这些差异表达的的基因的生物学意义进行解释？