胚胎干细胞研究及应用

胚胎干细胞研究及应用

摘要:胚胎干细胞是来源于早期胚胎内细胞团或原始生殖细胞的一种多能细胞，具有体外无限

复制和高度分化潜能的特性。目前，胚胎干细胞已逐渐应用胚胎干细胞体外测试与新药开发，移植治疗与细胞替代治疗，转基因动物与克隆上，并已取得了一定的研究成果。

关键词：胚胎干细胞; 生物学特性; 胚胎干细胞体外测试; 新药开发; 移植治疗; 细胞替代Embryonic stem cell research and application Abstract:Embryonic stem cells are derived from the early embryonic cells within the group or the original reproductive cells of a kind of pluripotent cells, has unlimited replication in vitro and the characteristics of high differentiation potential. At present, embryonic stem cells has been gradually used embryonic stem cells in vitro test and new drug development, transplantation and cell replacement therapy treatment, transgenic animals and clone, and have achieved some results.

Keywords: Embryonic stem cell; The biological characteristics; Embryonic stem cell in vitro test; New drug development; Transplantation therapy; Cell replacement threapy

目录

摘要……………………………………………………………………………………………………I Abstract………………………………………………………………………………………………I

第1章绪论.................................................................... III

1.1胚胎干细胞的概念 ..................................................................................................................................... III

1.2胚胎干细胞的发展简史. ............................................................................................................................ III

1.3胚胎干细胞的研究现况 ............................................................................................................................. III

第2章胚胎干细胞的生物学特性 ........................................................................................................................... V

2.1胚胎干细胞的形态学特征 .......................................................................................................................... V

2.2胚胎干细胞的分化 ...................................................................................................................................... V

第3章胚胎干细胞建系的技术要求 ........................................................................................................................ V I

3.1分化抑制物对胚胎干细胞的影响 ............................................................................................................. V I

3.2胚胎干细胞建系的技术要点..................................................................................................................... V I

3.2.1饲养层的制备和分化抑制物的选择 ............................................................................................. V I

3.2.2早期胚胎选择和PGCs的分离...................................................................................................... V I

3.2.3早期胚胎和PGCs的培养条件及胚胎干细胞的分离传代………………………………………VI

第4章胚胎干细胞的应用 .................................................................................................................................... V III

4.1胚胎干细胞体外测试 ............................................................................................................................... V III

4.2胚胎干细胞的新药开发…………………………………………………………………………………VIII

4.3胚胎干细胞的细胞治疗 ........................................................................................................................... V III

4.4生产转基因动物…………………………………………………………………………………………VIII

结论 ........................................................................................................................................................................... X

致谢 ............................................................................................................................................................................ XI

参考文献： ............................................................................................................................................................... XII