如何测定荧光量子效率

如何测定荧光量子效率

首先要有一个已知量子产率的标准物质，一般用硫酸奎宁。分别做标准物质和未知样品的吸收和荧光光谱。

① u=O s(Yu/Ys)(As/Au)

①u ----- 待求物质的荧光量子产率；

①s ―-准物质的荧光量子产率；

Yu ――待求物质的荧光积分面积；

Ys ――标准物质的荧光积分面积；

Au ――待求物质的荧光激发波长处的吸收值；

As ――标准物质的荧光激发波长处的吸收值。

测定激发光强度和荧光强度就可以了知道吧？

如果已知一标准物质的量子产率，可以配置相同浓度的标准和待测物质，在相同的测量条件下，测量两者的荧光强度•未知物质的荧光量子产率=F未知/F标准*标准的量子产率。

量子效率是指发射的光子数Nf与激发时吸收的光子数NX之比，即

量子效率=Nf/NX

我们知道，一般的发光总有一定的能量损失，激发光子的能量总是大于发射光子的能量.所以，量子效率通常都是小于100%.在实际测量时我们不好去测量发射的光子数Nf与激发时吸收的光子数NX,而通常用用下面的公式求算：

量子效率=发射峰的最大发光强度/激发峰的最大发光强度

但是注意根据待测物的激发和发射波长的范围，选择不同激发和发射波长的基准物。请问在有机电致发光中

关于量子效率中的外量子效率是如何计算的

公式是什么样子的

exp(eV/kT)=aP2+bP+c(1)

其中V为光生电压，k为玻尔兹曼常数，T为绝对温度，e为电子电荷，a，b，c为常数。

1. Additio nally, -NPN thin film emits bright blue fluoresce nee (461 nm)

with a quantum efficiency of 8.5%,higher than that of -NPD(5.5%). I 该化合物可发明亮的蓝色荧光(461 nm),积分球测定其薄膜荧光量子效率为8.5%, 比a-NPD的5.5%高。收藏指正

2. Their fluoresce nee emissi on properties were studied and their photolysis kin etics

was in vestigated in differe nt media by means of steady state photolysis method.

考察了其荧光光谱特性，发现甲氧基苯甲酰亚甲基光产碱剂的荧光量子效率较低，

而萘甲酰亚甲基光产碱剂较高。收藏指正

3. The fluoresce nee qua ntum efficie ncy of oxaz ine 1 perohlorate in 1,2- dichloro-etha ne was measured with photoacoustic speetroscopy in which the fluoresce nee que nchi ng wag used.

利用光声光谱技术，采取猝灭的方法对(口恶)嗪1高氯酸盐的二氯乙烷溶液进行荧光

量子效率的测量。收藏指正

4. The experimental results indicate that the quantum efficiency for the emission at 1.5 卩m is higher upon excitation at 0.98 卩m than that at 0.80 owi ng to the much weaker excited state absorpti on (ESA) compared with

those of excitation at 0.80 卩m.

研究表明：由于在0 .98卩谥激发下，激发态吸收较0 .80卩谥激发下小得多,因而其1.5 荧光发射量子效率也比0 .80 yn激发下高得多；收藏指正

5. Fluoresce nee property of coumari n derivative CND_(500) with 节cyclodextri n ( -CD) in aqueous solution and CND_(500) with ethyl alcohol,

n-saf Trit on X-100 micelle soluti on are exam ined by fluoresce nee method. It

is observed that the fluoresce nee peak, relative inten sity (F), polarizati on (P), relative quantum yield ((?)叮心匚

用荧光法研究了香豆素衍生物CND_(500)在伕CD水溶液中和在乙醇及非离子表面活性剂(n-Saf)Triton X-100等胶束溶液中的荧光特性,发现CND_(500)在伕CD 水溶液中的

荧光强度(F),荧光偏振度(P)、相对量子效率((?)收藏指正

6. Abstract: Polyphenylacetylenes were synthesized by [ Rn(nbd)Cl ] 2 and WCI6/Ph4S n catalyst systems in N2 at room temperature.Polymers with

high yield and high molecular weight were obtai ned.Their structures were characterized by UV,IR,NMR and GPC,respectively.The fluoresce nee of the polymer solutio n with differe nt concen tratio n was in vestigated using variable excitation wavelength from UV to visible region.The relation betwee n fluoresce nee

and polymer structure was studied.The results

showed that the molecular cha in structure has great in flue nee on fluoresce nee of the polymers.The emitt ing peak wavele ngth of fluoresce nee for the polymer of high stereoregularity is in depe ndent of solution concentration and excitation wavelength.The emitting peak wavele ngth of fluoresce nee for the polymer with disordered molecular cha in arran geme nt varies with cha nging the solutio n concen tratio n and excitati on wavele ngth,a nd differe nt emissi on sites in molecular cha ins and low emitti ng yield are show n. The measureme nt and an alysis of fluoresce nee spectroscopy of polyphe ny lacetyle nes may provide some useful information to judge the molecular chain arrangement in some

I打；

eon jugated polymers.

文摘:使用有机金属铑、金属钨/四苯基锡催化剂体系聚合苯乙炔，分别获得了高产率和高分子量的聚苯乙炔.使用UV, IR, NMR , GPC等分别对聚合物结构进行了

表征.采用不同波长的激发光对聚合物荧光性能进行研究，详细分析了聚合物结构与荧