四川部分晾晒烟种质遗传关系的SRAP分析

作物学报ACTA AGRONOMICA SINICA 2009, 35(1): 173−178 /zwxb/ ISSN 0496-3490; CODEN TSHPA9E-mail: xbzw@

本研究由四川省教育厅自然科学研究基金项目(2006B006), 四川农业大学青年科技创新基金项目(00130900)资助。*通讯作者(Corresponding author): 黄玉碧, E-mail: yubihuang@

Received(收稿日期): 2008-03-28; Accepted(接受日期): 2008-07-13.

DOI: 10.3724/SP.J.1006.2009.00173

四川部分晾晒烟种质遗传关系的SRAP分析

龙腾1刘雷2黄玉碧1,2,*

1 四川农业大学玉米研究所;

2 四川农业大学农学院, 四川雅安625014

摘要: 对25份四川地方性晾晒烟材料、1份黄花烟和1份普通烤烟材料进行了SRAP分析。共检测到3 368条扩增带, 其中特异性条带为998条, 平均多态检出率为29.6%。27个应试材料间遗传相似系数在0.26~0.97间, 平均为0.62, 表现出明显的遗传差异; 其中黄花烟、烤烟和晾晒烟3个栽培类型间遗传相似系数为0.38~0.78; 在25份晾晒烟品种间遗传相似性较高为0.75~0.97, 显示出种内的遗传基础相对比较狭窄, 但苍龙毛烟与其他供试材料有较大的遗传差异。经聚类分析, 可将黄花烟、烤烟和晾晒烟品种划分为3个栽培类别, 反映出栽培类型间的遗传差异; 进一步将25份晾晒烟材料分为3个亚类和2个单一品种的个类; 但按传统方法命名的多个毛烟品种和柳烟品种未被明显地分别聚为两类。SRAP标记技术能高效揭示亲缘关系很近的晾晒烟种质资源的遗传背景和亲缘关系。

关键词: 晾晒烟; 种质资源; 遗传关系; SRAP

Genetic Analysis of Sichuan Sun-Cured Tobacco Germplasm by SRAP LONG Teng 1, LIU Lei 2, and HUANG Yu-Bi 1,2,∗

1 Maize Research Institute of Sichuan Agricultural University; 2Agronomy College of Sichuan Agricultural University, Ya’an 625014, China Abstract: SRAP was used to analyze genetic relationships of 25 sun-cured tobacco varieties, one Nicotiana rustica variety, and one flue-cured tobacco variety collected from Sichuan province. PCR products were detected by polyacrylamide gel electrophore-sis (PAGE). A total of 3 368 DNA fragments were obtained, 998 of them were specific bands (polymorphic ratio was 29.6%), the average number of amplified DNA bands was 40 per primer. Genetic similarity ranged from 0.26 to 0.97 for all varieties, from 0.38 to 0.78 between Nicotiana rustica varieties, flue-cured tobacco varieties and sun-cured tobacco varieties, and from 0.75 to 0.97 among 25 sun-cured tobacco varieties. Cluster analysis was carried out with unweighted pair group method with arithmetic mean (UPGMA)．The results showed that all 27 varieties could be clustered into three groups, which revealed the genetic diversity among three cultivar-type tobacco germplasms. Furthermore, 25 sun-cured tobacco varieties could be classified into three sub-groups and two single varieties. Maoyan and Liuyan were two traditional famous groups of Sichuan sun-cured tobacco species. However, Maoyan and Liuyan varieties named by traditional method were not obviously grouped into two types. All results indi-cated that SRAP analysis may be used to reveal the genetic relationships among sun-cured tobacco varieties, providing a scientific basis for tobacco genetic research and breeding.

Keywords: Sun-cured tobacco; Germplasm; Genetic relationship; SARP

晾晒烟是生产雪茄烟和混合型卷烟的重要原料, 也是开发新式卷烟的原料资源。四川种植的地方性晾晒烟品种属于普通烟草亚属的普通烟草种(Nicotiana tabacum L.), 其典型代表毛烟、柳烟是中国特有的晾晒烟类型品种, 已于2003年被列入我国的“名晾晒烟名录”。然而, 长期以来四川省晾晒烟品种的引种、繁种、留种和命名等工作都是由烟农自己完成, 缺乏系统性的管理和记录。当地烟农往往根据烟草植株形态或叶片特征进行命名, 如将烟叶长宽比较大的品种称为柳烟, 将叶片多毛的品种称为毛烟; 这种命名方法极易造成品种命名混乱的现象, 如现有已知被冠以毛烟或柳烟的品种都各有10个左右。由于这些原因, 使得许多四川地方性晾晒烟的品种来源不明、品种间遗传关系不清, 这种状况已成为进一步改良和利用晾晒烟的限制性因素。

鉴定并分析晾晒烟种质的亲缘关系是加快晾晒烟品种改良和利用的基础。在种质鉴定和分析方面, 分子标记是在DNA分子水平上对产生的变异直接进行标记, 不受基因表达与否的限制, 具有信息量大、多态性强、