Agilent表达谱介绍

康成生物
KANGCHEN®
Excellence for Research
表达谱芯片应用
CNS Disease Immune diseases
Cardiovascular Disease Cancer research
康成生物
KANGCHEN®
Excellence for Research
表达谱芯片应用-有效辅助治疗的工具
5000个样本基因筛选
295例乳腺癌病例样本验证
筛选出70个基因的芯片产品化
产品化的芯片临床验证
康成生物
KANGCHEN®
Excellence for Research
Publication used by Agilent whole genome microarray
• • Regional Variation in Gene Expression in the Healthy Colon is Dysregulated in Ulcerative Colitis. Gut. 2008 Jun 3 Leucine-rich repeat receptor-like kinase1 is a key membrane-bound regulator of abscisic acid early signaling in Arabidopsis Plant Cell. 17 (4) , 2005 Hepatic Gene Expression Changes in Hypothyroid Juvenile Mice: Characterization of a Novel NegatiΒιβλιοθήκη Baidue Thyroid-Responsive Element Endocrinology 148(8), 2007 2-Methoxyestradiol Inhibits the Anaphase-Promoting Complex and Protein Translation in Human Breast Cancer Cells Cancer Res 67(2), 2007
康成生物
KANGCHEN®
Excellence for Research
基于mRNA水平的研究方法
芯片技术-高通量
康成生物
KANGCHEN®
Excellence for Research
KangChen ——Excellence for your Research
康成生物
KANGCHEN®
Excellence for Research
Thank you
康成生物
KANGCHEN®
Excellence for Research
Strict QC validation
康成生物
KANGCHEN®
Excellence for Research
The microarray quality control
康成生物
KANGCHEN®
Excellence for Research
Flexible of Agilent expression microarray
Genome-Whole Expression Microarrays Focused Pathway or Application-Specific Arrays
Pathway or Disease Survey
Study (Verification) of Individual Genes
Other Molecular Biology Techniques (Real-Time PCR)
eArray: Design your personal Microarrays !
康成生物
KANGCHEN®
Excellence for Research
Agilent’s Whole Solution
Flexible system
60-mer probe design (Identified optimized probe length)
康成生命科学实验中心 Service – from Sample to Results
全基因表达谱基因芯片技术服务
康成生物
KANGCHEN®
Excellence for Research
Pathway-Focused design
Application: Genome-Wide Survey
Methodology:
康成生物
KANGCHEN®
Excellence for Research
The microarray quality control—— Accuracy
康成生物
KANGCHEN®
Excellence for Research
The microarray quality control—— Accuracy
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康成生物
KANGCHEN®
Excellence for Research
严格的质量控制 • RNA浓度及完整性控制
• • Nanodrop 检测 甲醛琼脂糖变性凝胶电泳检测
• RNA标记效率的检测
芯片扫描质控标准
背景值均一 玻片没有划痕和污染 对照显著 红色通道和绿色通道荧光重叠
康成生物
KANGCHEN®
Summary Slide QC Differentially Expressed gene List GO analysis Pathway analysis
Raw data and target Hierarchical cluster date Scatter plot
Thank you for your attention
Probe Number Matrix Dye System Specification
44,000 Glass One color/two color 4 array per slide
康成生物
KANGCHEN®
Excellence for Research
The microarray quality control
Agilent whole genome expression microarray
康成生物
KANGCHEN®
Excellence for Research
Agilent whole genome expression microarray
Agilent E. coli M. grisea 2 yeast C. elegans Drosophila Frog Zebrafish Dog Sheep Pig Rhesus Monkey Arabidopsis Rice mosquito Salmon Tobacco Array format 8 x 15K 4 x 44K 4 x 44K 4 x 44K 4 x 44K 4 x 44K 4 x 44K 4 x 44K 8 x 15K 4 x 44K 4 x 44K 4 x 44K 4 x 44K 4 x 44K 4 x 44K 4 x 44K Genes coverage 10,000 15,170 6,256 40,000 13,936 21,000 43,000 42,000 15,000 40,000 18,000 40,000 42,000 26,000 43,000 20,000 Database source TIGR Comprehensive Microbial Resources database Fungal Genomics Laboratory database Saccharomyces Genome Database WormBase, RefSeq , Unigene, Ensembl, UCSC and TIGR FlyBase, BDGP, Unigene, Ensembl, UCSC and TIGR Gene Index databases Unigene, and TIGR RefSeq, Unigene Build, and TIGR Gene Index databases RefSeq, Unigene, Ensembl, and TIGR RefSeq (Release 27), Unigene RefSeq, Unigene, and TIGR the Macaca mulatta sequenced transcriptome the TIGR ATH1 v.5 database and Arabidopsis MPSS databases Rice Annotation Project Database RefSeq (Release 27), Ensembl (Release 49), Unigene (Build 10) and UCSC (anoGam1) RefSeq (Release 29), Unigene (Build 20) and TIGR (Release 14) RefSeq, TIGR and Unigene
Excellence for Research
专业的数据分析
• 康成将经过扫描之后的图像转成初始数字信号，经过标准 化处理之后导入Genespring软件中分析,最终筛选出具有 差异性表达的基因，并进行聚类、GO、Pathway等进一 步生物学分析，以便客户根据表达谱的研究结果开展下一 步深入研究 Final report