Protectin DX可以通过改善炎症和调节巨噬细胞表型提高脓毒症小鼠的存活率

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Protectin D X可以通过改善炎症和调节巨噬细胞表型

提局胺毒症小鼠的存活率

近期的一系列研究表明，源自Omega-3脂肪酸二十二碳六烯酸（docosahexaenoic acid, DHA)的脂质介质在多种炎症性疾 病中具有消炎或抗炎作用。因此，夏海发等试图评估Protectm DX(PDX;Pr〇tectm D1的异构体，一种新的脂质介质）是否可以 保护小鼠对抗脓毒症（sepsis),并探索其潜在机制。他们采用盲肠结扎穿孔（cecum ligation and puncture, CLP)建立脓毒症动 物模型，结果发现PDX可以提高脓毒症小鼠8d内的总体生存率，并减轻多器官损伤。除此之外，PDX可以在CLP术后24 h 减少促炎症因子和细菌载量。同时,PDX提高脓毒症小鼠腹腔巨噬细胞的吞噬能力，并增加M2型巨噬细胞的比例。在体外，PDX可以上调Raw264.7细胞中M2型巨噬细胞标志物（A rgl和Y m l)及其转录调节因子（过氧化物酶体增生物激活受体7,

peroxisome proliferator-activated receptor-7, PPAR-~y)的表达；反之，PPAR-~y 抑制剂 GW9662 和 PPAR-~y siRNA 可以消除 PDX 对 Raw264. 7细胞中A rgl和Y m l的诱导。综上所述，该研究结果表明PDX能够促进巨噬细胞的M2极化，增强其吞噬活性，加 快炎症消散，从而提高脓毒症小鼠的存活率。

Sci Rep, 2017, 7(1) :99(戴晓萌）