脾气虚论文：脾气虚变应性鼻炎模型益气脱敏汤嗜酸性粒细胞白细胞介素-5嗜酸细胞阳离子蛋白P物质

脾气虚论文：脾气虚变应性鼻炎模型益气脱敏汤嗜酸性粒细胞白细胞介素-5 嗜酸细胞阳离子蛋白 P物质【中文摘要】脾气虚是变应性鼻炎发病的主要病机,建立脾气虚变应性鼻炎病证复合模型,观察在脾气虚状态下变应性鼻炎的发病特点以及鼻腔黏膜的病理变化,运用健脾益气脱敏的中药进行药物干预,从病理、炎症介质的角度来评价益气脱敏汤对脾气虚变应性鼻炎的效应及作用机理,为临床运用益气脱敏法治疗变应性鼻炎提供实验依据。

方法：将健康SD大鼠随机分为正常对照组、变应性鼻炎(AR)组、脾气虚模型组、脾气虚AR组、脾气虚AR中药组。

AR组以卵白蛋白做为致敏原对大鼠进行实验性变应性鼻炎造模,脾气虚模型组利用大黄泻下法、饮食不节加劳倦过度综合因素的方法造模,经评价造模成功后进行AR模型的复制,复制病证结合之模型(脾气虚AR组)。

基础致敏成功后,再将脾气虚AR组随机分为：①脾气虚AR模型组,②脾气虚AR中药组。

大鼠鼻腔局部免疫激发前,脾气虚AR中药组,每日给予益气脱敏汤5.85g/kg,共7天,正常对照组与AR组、脾气虚组、脾气虚AR组给予等容积生理盐水。

除正常对照组、脾气虚组外,其他各组在给药结束后1小时再予以5%卵蛋白溶液激发；正常对照组、脾气虚组以生理盐水代替卵白蛋白。

造模第一天开始,观察动物一般情况及体重的变化,并在滴入卵白蛋白溶液30分钟内,着重观察鼻部症状。

于末次鼻腔局部激发后30分钟,大鼠眼眶取血,观察血中及鼻分泌物嗜酸粒细胞(EOS),留血清待测白细胞介素5(IL-5)、嗜酸性细胞阳离子蛋白(ECP)的含量。

处死动物,剥离上颌骨摘取鼻中隔黏膜多聚甲醛固定,HE染色作组织病理学检查,观察在光镜下的病理形态变化；采用免疫组化法检测鼻中隔黏膜P物质(SP)在脾气虚型变应性鼻炎大鼠鼻黏膜中的表达变化。

结果：观察AR组、脾气虚AR组动物出现的鼻部症状(鼻痒、喷嚏、清涕),鼻分泌物及血中EOS数量,被动皮肤免疫反应(PCA)试验作用明显,大鼠血清中IL-5、ECP含量升高(P<0.01)；鼻黏膜间质有较多EOS及少量淋巴细胞浸润,血管扩张充血；SP的表达均增高,与AR组相比,脾气虚AR组鼻中隔黏膜SP表达有进一步增强的趋势,而经过益气脱敏中药干预治疗后,改善了大鼠脾气虚症状,AR症状减轻,大鼠血清中IL-5、ECP含量降低(P<0.01)；明显降低大鼠鼻黏膜EOS浸润的病理状态及SP的表达。

结论：①利用大黄泻下、饮食不节加劳倦过度法及以卵白蛋白为致敏原的复合方法,建立脾气虚变应性鼻炎大鼠病证结合模型是可行的。

在同样致敏原的作用下,脾气虚模型动物更易于发生变应性鼻炎。

②益气脱敏汤对脾气虚变应性鼻炎模型大鼠有一定治疗效应,其疗效的产生与该方改善鼻局部黏膜病理炎症及SP表达、降低EOS、及血清IL-5、ECP的含量有关,这一结果也反证了脾气虚造模的成功。

【英文摘要】:According to Traditional Chinese Medicine (TCM), spleen qi deficiency (SQD) is purported to be the main pathogenesis of allergic rhinitis (AR), establish onspleen-deficiency disease and syndrome compound model of allergic rhinitis, to observe on spleen-deficiency condition in the morbidity of allergic rhinitis’characteristics and thenasal mucosa’s pathological changes. Using traditional Chinese medicine of tonifying the spleen and the qi and desensitization to drug intervention, which from pathology, inflammation angle to evaluate the effect of Yiqi Tuoming Prescription on spleen-deficiency allergic rhinitis and function mechanism. Providing the basis for clinical use of yi-qi desensitization method to treat allergic rhinitis.Methods:Normal SD rats were randomly divided into normal controls, allergic rhinitis (AR) group, spleen qi deficiency (SQD) model group, spleen qi deficiency combined with allergic rhinitis (AR+SQD) and AR+SQD treated with TCM. The AR group was modeled by using ovalbumin as the sensibiligen. The SQD group was underwent rhubarb purgation, eating and drinking without temperance and overstrains. The AR+SQD group was made by combining ovalbumin injection with Rhubarb purgation, eating and drinking without temperance and overstrains. After the succeed of modeling, divide the AR+SQD group randomly into AR+SQD group and AR+SQD treated with TCM. Before the sdtimulation of part immunization in the nasal cavity, The AR+SQD+TCM group were give TCM treatment (rats given Yiqi Tuoming prescription of 5.58g/kg/day for seven days). The control group、the AR group、the SQD group and the AR+SQD group were given equal amount ofnormal saline. Except the normal controls and the SQD group, the other groups were stimulated an hour after giving the drug by 5% ovalbumin solution. The normal controls and the SQD group used the normal saline instead of ovalbumin.From the first day of the modeling, Observe the change of the weight and the common condition on the rats. And especially watch the symptom in the nasal in the period of 30 minutes driping the ovalbumin solution.30 minutes after the last stimulation in the nasal cavity, take the blood from the rats’eye socket, survery the Eosinophils levels, detective the content of interleukine 5 (IL5) and eosinophil cationic protein (ECP) in serum. Execut the animal, strip the maxilla and get the nasal septum mucous membrane, fastened by paraformaldehyde, do the HE staining before pathological morphology. Watch the change of pathomorphism by light microscope. Test the Substance P from nasal septum mucous membrane by Immunohistochemical measurement in the SQD group.Results:The rats in AR and AR+SQD groups were positive for symptoms in the nasal(itch、sneeze、nasal discharge), the EOS in nasal mucus and blood、passive cutaneous anaphylaxis (PCA). The measurements showed increased IL5 and ECP in serum compared with control (P<0.01).There were more EOS and less lymphocyte soaking with vasodilatation andhyperemia in nasal septum mucous membrane. SP rised in allgroups, the expression of substance P was increased in AR+SQDanimals compared to AR animals. After the treated of TCM, the symptoms of SQD were improved, the symptoms of AR were lightened, increased IL5 and ECP in serum compared with control (P<0.01).The soaking of EOS in nasal septum mucous membrane andexpression of substance P were obviousllylightened.Conclusion:(1)It is feasible that use rhubarbpurgation, eating and drinking without temperance andoverstrains and ovalbumin as the sensibiligen to build themodel of AR+SQD. The animals with SQD easier occur AR than thenormal animals. (2) Yiqi Tuoming prescription was effective intreating the rats with AR+SQD. The effection is related toimprove inflammation in part of nasal septum mucous membraneand decrease the content of interleukin-5, eosinophil cationic protein, and substance P. This result also reverse proved thesucceed of building the model of SQD.【关键词】脾气虚变应性鼻炎模型益气脱敏汤嗜酸性粒细胞白细胞介素-5 嗜酸细胞阳离子蛋白 P物质【采买全文】1.3.9.9.38.8.4.8 1.3.8.1.13.7.2.1 同时提供论文写作一对一辅导和论文发表服务.保过包发.【说明】本文仅为中国学术文献总库合作提供，无涉版权。