Effects of umbilical cord blood epc following myocardial infarction

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心肌梗死后心肌组织内环境对移植干细胞心肌内存活、分化影响

心肌梗死后心肌组织内环境对移植干细胞心肌内存活、分化影响艾旗;袁春菊【摘要】近年实验研究与初步临床研究表明,包括骨髓间充质干细胞等干细胞移植治疗急性心肌梗死,可减少心肌梗死体积,促进心肌梗死区域血运重建,增加有功能的心肌细胞数量,并改善心功能,为心肌梗死的治疗开辟了一条新途径.但干细胞移植治疗心肌梗死总体疗效有限,其重要原因之一是移植干细胞在心肌内存活率低、定向分化不足.近期国内外研究证实,心肌梗死后心肌组织内环境是移植干细胞在心肌内存活、分化的重要影响因素.【期刊名称】《医学综述》【年(卷),期】2014(020)021【总页数】3页(P3908-3910)【关键词】心肌梗死;干细胞;心肌内环境;存活;分化【作者】艾旗;袁春菊【作者单位】中南大学湘雅医院心内科,长沙410008;中南大学湘雅医院心内科,长沙410008【正文语种】中文【中图分类】R542.22目前多个实验及临床研究[1-2]表明，经心肌、冠状动脉及静脉三类干细胞移植途径治疗急性心肌梗死，干细胞在心肌内存活、分化不足，心功能增加仅达10%，甚至近期国外实验与临床研究报道的结论为阴性结果[3-4]。

干细胞在心肌内存活、分化不足使干细胞移植疗法停滞不前。

因此，如何增加干细胞在心肌内存活、分化，提高干细胞移植治疗心肌梗死疗效是目前急需解决的主要问题。

1 心肌梗死后心肌内环境变化心肌内环境对干细胞在心肌内存活、分化起决定性作用。

心肌内环境是心肌细胞赖以生存的环境，心肌细胞、间质细胞、成纤维细胞产生或分泌的细胞外基质成分及某些生物活性分子,对心肌细胞发挥支持、连接、营养和保护等作用[5]。

急性心肌梗死发生后，继发于心肌细胞坏死，外周血中性粒细胞和单核细胞短时间内募集到受损坏死心肌组织。

心肌梗死急性期心肌组织内微环境突出表现为大量中性粒细胞、单核细胞浸润和肿瘤坏死因子α、白细胞介素(interleukin,IL)1β、IL-6等促炎因子显著增加。

碧云天细胞计数试剂盒CCK-8说明书

碧云天生物技术/Beyotime Biotechnology 订货热线：400-168-3301或800-8283301 订货e-mail ：****************** 技术咨询：***************** 网址：碧云天网站微信公众号Cell Counting Kit-8 (CCK-8试剂盒)产品编号产品名称包装 C0038Cell Counting Kit-8 (CCK-8试剂盒)500次产品简介：Cell Counting Kit-8，简称CCK-8试剂盒或CCK8试剂盒，是一种基于WST-8而广泛应用于细胞增殖和细胞毒性的快速、高灵敏度检测的试剂盒。

WST-8是一种类似于MTT 的化合物，在电子耦合试剂存在的情况下，可以被线粒体内的一些脱氢酶还原生成橙黄色的formazan (参考图1)。

细胞增殖越多越快，则颜色越深；细胞毒性越大，则颜色越浅。

对于同样的细胞，颜色的深浅和细胞数目呈线性关系。

图1. WST-8检测原理图 (EC=electron coupling reagent ，即电子耦合试剂)WST-8是MTT 的一种升级替代产品，和MTT 或其它MTT 类似产品如XTT 、MTS 等相比有明显的优点。

首先，MTT 被线粒体内的一些脱氢酶还原生成的formazan 不是水溶性的，需要有特定的溶解液来溶解；而WST-8和XTT 、MTS 产生的formazan 都是水溶性的，可以省去后续的溶解步骤。

其次，WST-8产生的formazan 比XTT 和MTS 产生的formazan 更易溶解。

再次，WST-8比XTT 和MTS 更加稳定，使实验结果更加稳定。

另外，WST-8和MTT 、XTT 等相比线性范围更宽，灵敏度更高。

WST-8和WST-1相比，检测灵敏度更高，更易溶解，并且更加稳定。

本试剂盒可以用于细胞因子等诱导的细胞增殖检测，也可以用于抗癌药物等对细胞有毒试剂诱导的细胞毒性检测，或一些药物诱导的细胞生长抑制检测。

间充质干细胞治疗膝骨关节炎的临床研究进展

间充质干细胞治疗膝骨关节炎的临床研究进展2.天津博纳戈恩生物科技有限公司，天津 300042；摘要：膝骨关节炎是一种以退行性病理改变为基础的疾患，发病率随年龄增大而升高，因此常见于中老年人群。

目前通常的治疗手段是通过消除炎症或减轻疼痛来缓解症状，从而阻止和延缓疾病的发展，进而保护关节功能，以防功能丧失，然而现有的临床治疗方式远期效果大多并不理想。

近年来随着对间充质干细胞的研究逐步深入，人们发现其可促进软骨再生的特点，因而以间充质干细胞移植为主的治疗方法，在国内外逐渐兴起，并开始进入临床实验阶段。

本文将主要就间充质干细胞治疗膝骨关节炎的原理、以及相关临床研究进行汇总，并为膝骨关节炎相关的研究和间充质干细胞的应用提供参考。

关键词：膝骨关节炎；间充质干细胞；临床研究；自体；同种异体前言膝骨关节炎（Knee Osteoarthritis, KOA）是一种以退行性病理改变为基础的疾患，常见于中老年人群。

该疾病初期症状较轻，临床表现多为膝盖部位肿胀、酸痛、行动不适，坐立姿势改变时疼痛、弹响等，严重时出现活动受限、积液、关节畸形，不及时得到有效治疗病情可能发展为残疾[1]。

KOA常由长期姿势僵化、劳累、外伤、或其它关节退行性病变如软骨退化、半月板磨损等原因导致。

因此该病的治疗关键在于保护软骨，防止进一步受损，同时尽可能使其自我修复和再生。

但由于膝关节部位软骨组织无血管，因此软骨部位自我修复和再生的能力很差，且关节软骨缺损的再生方法很少[2]。

KOA一般采用综合治疗，包括病人教育，药物治疗，理疗或外科手术治疗，现有的治疗方式包括软骨保护剂硫酸氨基葡萄糖、透明质酸关节腔注射、中医针灸推拿、膝关节置换以及间充质干细胞治疗等，其远期效果大多并不理想，而近年来随着对间充质干细胞的研究逐步深入，人们发现其可促进软骨再生的特点，因而以间充质干细胞移植为主的治疗方法，在国内外逐渐兴起，并开始进入临床实验阶段。

本综述将主要就间充质干细胞治疗KOA的原理、以及相关临床研究进行汇总，并为KOA相关的研究和间充质干细胞的应用提供参考。

SPM促脑发育训练介导脑剪切力通路对缺氧缺血性脑病的作用机制研究

SPM促脑发育训练介导脑剪切力通路对缺氧缺血性脑病的作用机制研究（1.哈尔滨医科大学附属第五医院，黑龙江大庆 163000）（2.哈尔滨医科大学大庆校区，黑龙江大庆 163000）摘要：目的探究新生儿缺氧缺血性脑病病理机制中脑血管内皮的功能，或许与SPM促脑发育训练增加脑血管剪切力介导的PECAM-1/eNOS/NO通路调控相关联，并最终实现了脑保护作用。

方法选择成年雄性蒙古沙鼠（60-80 克），沙鼠腹腔注射戊巴比妥钠1% (v/w)麻醉，夹闭双侧颈总动脉5分钟诱导缺血，后松解恢复血流。

不同组采用SPM促脑发育训练手段，最终分别检测各组的组织形态、蛋白及凋亡检测等相关数据。

结果 SPM促脑发育训练可以增加剪切力，继而作用于PECAM-1/eNOS/NO通路影响脑血管内皮功能，内皮功能改善有助于缺氧缺血性脑病的康复。

结论 SPM促脑发育训练增加脑剪切力，剪切力通过PECAM-1/eNOS/NO通路改善血管内皮功能，继而改善缺氧缺血性脑病介导的脑损伤。

关键词：缺氧缺血性脑病；PECAM-1/eNOS/NO；剪切力；脑损伤SPM brain development training mediates the effects of brain shear stress pathway on the mechanism of hypoxic ischemic encephalopathyAbstract: Objective To explore the function of cerebral vascular endothelium in the pathological mechanism of neonatal hypoxic ischemic encephalopathy, which may be related to the regulation of PECAM-1/eNOS/NO pathway mediated by SPM promoting brain development training and increasing cerebral vascular shear force, and ultimately achieve brain protection. Methods Adult male Mongolian gerbils (60-80g) were selected and anesthetized by intraperitoneal injection ofpentobarbital sodium 1% (v/w). Bilateral common carotid arteries wereoccluded for 5 minutes to induce ischemia, and then released torestore blood flow. Different groups were trained with SPM to promote brain development, and finally relevant data such as tissue morphology, protein, and apoptosis detection were detected in each group. Results SPM brain development training can increase shear stress, which inturn acts on the PECAM-1/eNOS/NO pathway and affects the endothelial function of cerebral blood vessels. The improvement of endothelial function is conducive to the rehabilitation of hypoxic ischemic encephalopathy. Conclusion SPM brain development training increases brain shear force, which improves vascular endothelial functionthrough PECAM-1/eNOS/NO pathway, thereby improving brain injury mediated by hypoxic ischemic encephalopathy.Keywords: Hypoxic ischemic encephalopathy; PECAM-1/eNOS/NO； Shear force; brain damage新生儿缺氧缺血性脑病（HIE）是新生儿科常见多发疾病[1]，当前较为公认的HIE病理学说包括：神经元细胞钠-钾ATP泵障碍导致的细胞毒性水肿[2]；钙离子通道异常所致的钙离子超载[3]；氧化应激过激反应介导的损伤[4]；兴奋性氨基酸的持续性释放[5]；在HIE的病理过程中上述机制最终导致了神经元细胞的凋亡和坏死[6]，继而诱发不同程度的临床症状和体征，严重的HIE可致患儿死亡，幸存者仍遗留严重的永久性神经系统损害[7]，如智力低下、运动障碍、脑瘫及癫痫等。

美国妇产科协会关于脐带血库专家意见——Umbilical Cord Blood Banking

VOL. 126, NO. 6, DECEMBER 2015 OBSTETRICS & GYNECOLOGY e127Umbilical Cord Blood BankingABSTRACT: Once considered a waste product that was discarded with the placenta, umbilical cord blood is now known to contain potentially life-saving hematopoietic stem cells. When used in hematopoietic stem cell transplantation, umbilical cord blood offers several distinct advantages over bone marrow or peripheral stem cells. However, umbilical cord blood collection is not part of routine obstetric care and is not medically indicated. Umbilical cord blood collection should not compromise obstetric or neonatal care or alter routine practice for the timing of umbilical cord clamping. If a patient requests information on umbilical cord blood banking, balanced and accurate information regarding the advantages and disadvantages of public and private umbilical cord blood bank-ing should be provided. The routine storage of umbilical cord blood as “biologic insurance” against future disease is not recommended.RecommendationsThe American College of Obstetricians and Gynecologists makes the following recommendations regarding umbili-cal cord blood banking:• Umbilical cord blood collection should not com-promise obstetric or neonatal care or alter routine practice for the timing of umbilical cord clamping. • If a patient requests information on umbilical cordblood banking, balanced and accurate information regarding the advantages and disadvantages of public and private umbilical cord blood banking should be provided.• The current indications for cord blood transplant arelimited to select genetic, hematologic, and malignant disorders.• Patients should be aw are that in certain instances,use of one’s own stem cells is contraindicated. Most conditions potentially treated by a patient’s ow n umbilical cord blood already exist in his or her own cells and, therefore, the stored blood cannot be used to treat the same individual. • Counseling should include disclosure that the chancea child or family member develops a condition that could be treated w ith an autologous transfusion of umbilical blood is rare.• The routine storage of umbilical cord blood as “bio-logic insurance” against future disease is not recom-mended.• Directed cord blood banking is available throughprivate and public umbilical cord blood banks for any pregnant patient who has a family member with a disease potentially treated by hematopoietic stem cell transplant.• Some states have passed legislation requiring physi-cians to inform their patients about umbilical cord blood banking options. Obstetrician–gynecologists and other obstetric care providers should consult their state medical associations for more information regarding state laws.• As a variety of circumstances may arise during theprocess of labor and delivery that may preclude adequate collection, it is important to obtain w ell-documented informed consent that various medicalC OMMITTEE OPINIONNumber 648 • December 2015 (Replaces Committee Opinion Number 399, February 2008)Committee on GeneticsCommittee on Obstetric PracticeThis document reflects emerging clinical and scientific advances as of the date issued and is subject to change. The information shouldnot be construed as dictating an exclusive course of treatment or procedure to be followed.The American College ofObstetricians and GynecologistsWOMEN’S HEALTH CARE PHYSICIANScircumstances of the mother or the neonate may prevent umbilical cord blood collection.• Physicians or other professionals who recruit preg-nant women and their families for for-profit umbili-cal cord blood banking should disclose any financial interests or other potential conflicts of interest. IntroductionOnce considered a w aste product that w as discarded with the placenta, umbilical cord blood is now known to contain potentially life-saving hematopoietic stem cells. When used in hematopoietic stem cell transplantation, umbilical cord blood offers several distinct advantages over bone marrow or peripheral stem cells. Biologically, a greater degree of human leukocyte antigen mismatch is tolerated by the recipient and the incidence of acute graft-versus-host reaction is decreased w hen umbilical cord blood is used compared with unrelated donor bone marrow (1, 2). The predominant disadvantage of umbili-cal cord blood use is that there is often a low yield of stem cells acquired per unit. Only 8–12% of umbilical cord blood units have sufficient cell volume for transplant to a person weighing 80 kg (176 lb) (3). However, the use of combined units of umbilical cord blood allows for the expansion of umbilical cord blood volume (and increased number of stem cells) to be used for adult hematopoi-etic transplants. Since the first successful umbilical cord blood transplant in 1988, it has been estimated that more than 30,000 transplants have been performed in children and adults for the correction of inborn errors of metabolism, hematopoietic malignancies, and genetic disorders of the blood and immune system (4). Umbilical cord blood stem cells also are being studied in the areas of regenerative medicine and infectious disease (www. ).Public Versus Private Umbilical Cord Blood BankingTwo types of banks have emerged for the collection and storage of umbilical cord blood: 1) public banks and 2) private banks. The first public bank w as established at the New York Blood Center in 1991 and other public banks have since been established in various regions of the country. In December 2005, federal legislation, the C.W. Bill Young Cell Transplantation Act, w as enacted that provides funding for continued growth of a national umbilical cord blood registry in the United States. Some states have passed legislation requiring physicians to inform their patients about umbilical cord blood banking options. Obstetrician–gynecologists and other obstetric care providers should consult their state medical associa-tions for more information regarding state laws.Public banks promote allogeneic (related or unre-lated) donation, analogous to the current collection of whole blood units in the United States. These banks typi-cally are associated with a local network of obstetric hos-pitals that send their units of blood to a central processing facility. A minority of public banks w ill accept units through shipment by an overnight express courier (5).A list of participating hospitals is maintained by the National Marrow Donor Program (6). Public banks are supported through government grants, private dona-tions, and compensation for cord blood units used for transplant. Units of umbilical cord blood collected for public banks must meet rigorous standards of donor screening and infectious disease testing as outlined by the U.S. Food and Drug Administration. As of October 20, 2011, every unrelated donor cord blood unit to be transplanted in the United States must be either licensed or covered under an investigational new drug application approved by the U.S. Food and Drug Administration (7). Initial human leukocyte antigen typing of these units allows them to be entered into computerized registries so that when the need arises, a specific unit can be rapidly located for a patient.Private for-profit banks w ere initially developed to store stem cells from umbilical cord blood for autologous use (taken from an individual for subsequent use by the same individual) if the child develops disease later in life or for use by other family members. Private banks adver-tise directly to consumers often encouraging parents to bank their infants’ cord blood as a form of “biological insurance.” The routine storage of umbilical cord blood as biological insurance against future disease is not rec-ommended by the American Academy of Pediatrics, given the lack of scientific data to support its use and availability of allogeneic transplantation (8). Physicians or other professionals who recruit pregnant women and their families for for-profit umbilical cord blood banking should disclose any financial interests or other potential conflicts of interest.Considerations for Patients Regarding Umbilical Cord Blood BankingIf a patient requests information about umbilical cord blood banking, balanced and accurate information regarding the advantages and disadvantages of public and private banking should be provided. Patients should be aware that in certain instances, use of one’s own stem cells is contraindicated. Most conditions potentially treated by a patient’s own umbilical cord blood already exist in his or her own cells and, therefore, the stored blood can-not be used to treat the same individual. The chance of an autologous unit of umbilical cord blood being used for a child or a family member is remote, unless a fam-ily member is know n to have a medical condition that could be treated with transplant, and this fact should be disclosed to the patient (9). Directed cord blood banking should be encouraged when there is knowledge of a full sibling in the family with a medical condition (malignant or genetic) that could potentially benefit from cord blood transplantation. Patients should be made aw are of the financial obligation for processing and annual storage feese128Committee Opinion Umbilical Cord Blood Banking OBSTETRICS & GYNECOLOGYpopulations are significantly underrepresented in publicbanks. Families may consider the societal benefit frompublic umbilical cord blood donation to increase thechance for all groups of finding a matched cord bloodunit.Technique and Informed ConsentTo ensure that there will be enough cells for transplan-tation, at least 40 mL of cord blood must be collected.Collection can be performed before or after removing theplacenta. In either case, thorough cleansing of a section ofumbilical cord is performed, and blood is obtained fromthe umbilical vein by venipuncture and allowed to drainby gravity into a bag supplied by the bank. Blood shouldbe collected as soon as feasible after birth to minimizecoagulation and maximize volume (10). If the specimenis not sterile or is not of sufficient quantity, it will be dis-carded by the bank.Umbilical cord blood collection is not part of routineobstetric care and is not medically indicated. Umbilicalcord blood collection should not compromise obstetricor neonatal care or alter routine practice for the timing ofumbilical cord clamping. A variety of circumstances mayarise during the process of labor and delivery that maypreclude adequate collection. Therefore, it is importantto obtain well-documented informed consent that vari-ous medical circumstances of the mother or neonate mayprevent umbilical cord blood collection.For More InformationThese resources are for information only and are not meant to be compre-hensive. Referral to these resources does not imply the American Collegeof Obstetricians and Gynecologists’ endorsement of the organization, theorganization’s web site, or the content of the resource. The resources maychange without notice.ACOG has identified additional resources on topicsrelated to this document that may be helpful for ob-gyns,other health care providers, and patients. You may viewthese resources at /More-Info/CordBloodBanking.References1. Laughlin MJ, Eapen M, Rubinstein P, Wagner JE, Zhang MJ,Champlin RE, et al. Outcomes after transplantation of cordblood or bone marrow from unrelated donors in adultswith leukemia. N Engl J Med 2004;351:2265–75. [PubMed][Full Text] AVOL. 126, NO. 6, DECEMBER 2015Committee Opinion Umbilical Cord Blood Banking e129。

中、美脐带血造血干细胞质量控制标准比对分析

标准比对中、美脐带血造血干细胞质量控制标准比对分析■ 曾庆想1 李婵2 李佩芳1 徐绍坤2〔1. 个体化细胞治疗技术国家地方联合工程实验室（深圳）；2. 深圳市北科生物科技有限公司〕摘要：脐带血造血干细胞移植技术在人类血液疾病、先天性疾病方面的应用越来越多，而作为一种直接输注入人体的产品，脐带血造血干细胞的质量控制必须考虑采集、运输、制备等各个环节存在的风险。

本文研究了中、美两国在脐带血造血干细胞质量控制方面的标准现状，并对两国相关的主要质量控制标准检测指标进行对比分析，从标准化的角度找出两国之间的差异并提出参考建议。

关键词：脐带血造血干细胞，质量，标准比对DOI编码：10.3969/j.issn.1002-5944.2021.15.029Comparative Study of Quality Control Standards of Umbilical Cord Blood Stem Cells between China and the United StatesZENG Qing-xiang1 LI Chan2 LI Pei-fang1 XU Shao-kun2（1. National-local Associated Engineering Laboratory for Personalized Cellular Therapy (Shenzhen);2. Shenzhen Beike Biotechnology Co., Ltd.）Abstract: Umbilical cord blood hematopoietic stem cell (UCB-HSC) transplantation technology has been widely used in human blood diseases and congenital diseases. As a kind of product directly injected into human body, the quality control of umbilical cord blood stem cells must consider the risks of collection, transportation, preparation and other aspects. This paper studies the current situation of quality control standards of umbilical cord blood stem cells in China and the United States, compares the quality control standards in the two countries, finds out the differences between the two countries from the perspective of standardization, and puts forward some suggestions.Keywords: UCB-HSC, quality, standard comparison1 背景1974年，科学家Knudtzon首次发现在脐带血中存在造血干细胞。

新生儿脐带血气分析正常值【脐带血气分析】

新生儿脐带血气分析正常值【脐带血气分析】脐带血气分析脐血气分析已经成为一个评估婴儿出生状态时候的方法。

在有些医疗机构，脐血气测定已经成为常规。

美国妇产科学院（xx）推荐在新生儿Apgar评分低的时候使用。

尽管脐血气无论是对即刻和远期的神经系统损伤均不具有良好的预测价值，但是对于理解导致酸中毒的产中以及分娩事件是有帮助的。

表D-1列出了足月和早产儿脐血气动脉血的正常值（均值±标准差）。

脐带血的收集在分娩后立即用2把钳子钳夹胎儿侧的脐带，另外2把钳子钳夹胎盘侧，切断后，留取10～20cm长度的脐带。

把血从脐动脉里面留到一个1～2ml肝素化了的注射器里面，加上针帽，注射器放到一个加有碎冰的塑料盒里面，立即转运到实验室。

胎儿酸中毒的生理胎儿可以通过胎盘迅速清除CO2，如果CO2不能快速得到清除，H2CO3（碳酸）在体内蓄积，就会导致呼吸性酸中毒。

由机体厌氧代谢产生的有机酸，包括乳酸和β羟基丁酸从胎儿血中清除缓慢，蓄积造成了代谢性酸中毒。

随着代谢性酸中毒的发展，碳酸氢根（HCO3）因与有机酸缓冲结合而下降。

随着H2CO3和有机酸（表现为HCO3下降）的增加，表现为混合性呼吸性——代谢性酸中毒。

为了临床的目的，HCO3代表的是代谢性的成分，以mEq/L报告，H2CO3浓度代表的是呼吸性的成分，以PCO2来报告，单位为mmHg。

碱剩余是用来衡量HCO3的缓冲能力的，譬如，随着代谢性酸中毒的加重，HCO3将下降以维持正常的pH值。

碱不足是指在HCO3浓度下降至正常水平以下，碱过多是指HCO3高于正常。

呼吸性酸中毒呼吸性酸中毒通常是在胎盘气体交换急性中断时发生的，继发CO2潴留。

脐带一过性受压是发生胎儿呼吸性酸中毒的最常见事件。

通常情况下，呼吸性酸中毒不会对胎儿产生损害，表D-2显示了呼吸性酸中毒时脐动脉血每一成分的阈值。

代谢性酸中毒代谢性酸中毒是在氧气供应无法满足胎儿细胞能量需要的厌氧代谢所需要的时间和幅度时产生的，代谢性酸中毒和缺血缺氧性脑病以及新生儿的功能障碍发生有关，但是即便是重度的代谢性酸中毒也不能预测继发的脑瘫。

脐带血造血干细胞的采集、浓缩与低温冷冻保存

脐带血造血干细胞的采集、浓缩与低温冷冻保存背景：日期：2010-5-11 作者：佚名编辑：exiber 点击次数：15 销售价格：免费论文论文编号：lw201005111438582963论文字数：8000论文属性：职称论文论文地区：中国论文语种：中文收藏： google书签雅虎搜藏百度搜藏新浪vivi 和讯网摘poco网摘天极网摘qq书签饭否mister-wong365网摘LiveDiggDiglog关键词：胎血低温保藏造血干细胞中国论文职称论文P>随着CBT的临床应用推动了CBB的建立。

截止到1998年,美国纽约中心冻存CB已超过8 000份,提供临床应用600余份[2]。

CB已成为骨髓和外周血后的第3种造血干细胞来源,日益受到人们的重视,CBB在全世界范围内广泛建立起来。

成为近十年来造血干细胞移植领域中的重要进展之一。

CBB的建立涉及到脐血的采集、分离、冷冻、复苏、检测、建库等诸多环节。

1997年至2000年5月,我们已冻存经HLA检测的CB 3 744份。

CB采集量是CBB的重要指标之一。

CBB量多,造血干细胞含量多,移植效果好。

伦敦CBB 1998年采集量为1 000份样本,平均666份CB采集量为(70±23) ml(40~181 ml)[3]。

本研究根据国内外文献建立了一套CB采集的分离技术。

通常CB的采集分为胎盘娩出本论文由无忧论文网整理提供前和娩出后采集,有文献报道胎盘娩出后所采集的CB有核细胞数低于胎盘娩前所采集到的有核细胞数[4]。

本研究采用的是胎盘娩前采集的方法,平均采集量为(93±22) ml(45~198 ml),平均采集到的有核细胞数为11.2×108,且采集过程对母亲和新生儿均无影响。

同时我们也观察到采集的CB中的有核细胞数与CB量之间存在线性相关,r=0.67,P<0.01。

因此,尽可能多的采集CB量可增加采集到的有核细胞数。

碧云天生物技术碱性磷酸酶检测试剂盒产品说明书

碧云天生物技术/Beyotime Biotechnology 订货热线：400-1683301或800-8283301 订货e-mail ：******************技术咨询：*****************网址：碧云天网站微信公众号碱性磷酸酶检测试剂盒产品编号产品名称包装 P0321S 碱性磷酸酶检测试剂盒 100次 P0321M碱性磷酸酶检测试剂盒500次产品简介：碧云天生产的碱性磷酸酶检测试剂盒(Alkaline Phosphatase Assay Kit)是一种用于快速、便捷地检测细胞或组织样品的裂解或匀浆产物的上清液、血清、血浆、尿液等样品中内源性的碱性磷酸酶活性的试剂盒。

碱性磷酸酶(Alkaline Phosphatase, AP/ALP/AKP/ALKP/ALPase/Alk Phos)也称碱性磷酸酯酶(EC 3.1.3.1)，可以在碱性条件下催化磷酸酯键的水解。

哺乳动物中，肝脏、胆管、肾脏、骨头和胎盘中的碱性磷酸酶活性比较高。

常见的碱性磷酸酶包括肠道碱性磷酸酶(alkaline phosphatase, intestinal, ALPI)、非组织特异性碱性磷酸酶(alkaline phosphatase, tissue-nonspecific isozyme, ALPL)和胎盘碱性磷酸酶(alkaline phosphatase, placental type, 也称placental alkaline phosphatase, PLAP)。

常见的小牛肠碱性磷酸酶(Calf Intestinal Alkaline Phosphatase, CIAP/CIP)被广泛用于二抗等的标记最终用于蛋白和核酸等的检测，也常用于DNA 或RNA 5´和3´末端的去磷酸化(去单磷酸化)，特别是质粒的5´末端去磷酸化以避免质粒自连等。

干细胞，如iPS 中，碱性磷酸酶的活性很高，常被用作iPS 成功诱导的标志。

水中分娩与常规分娩脐带血采集质量的比较分析

水中分娩与常规分娩脐带血采集质量的比较分析（作者:___________单位: ___________邮编: ___________）作者：陈亮，沈丽敏，王峰蕾，陆丽宏，李冉，高峰【摘要】目的探讨水中分娩和常规分娩两种不同分娩方式对脐带血采集质量的影响。

方法收集200例产妇作为入选对象，其中100例为水中分娩组，对应100例常规分娩作为对照组。

收集两种不同分娩方式的脐带血，比较两者脐带血采集的质量。

结果水中分娩方式采集的脐带血采集量、有核细胞数、细菌污染率和CD34+造血干细胞数及活性均优于常规分娩方式采集的脐带血。

结论分娩方式是影响脐带血质量的重要因素，水中分娩是提高脐带血采集质量的有效分娩方法，可为临床移植提供更高质量的脐带血造血干细胞。

【关键词】脐带血采集方法;水中分娩;脐带血质量【Abstract】 Objective To evaluate the effect of delivery style (water delivery or conventional delivery) on the quality of umbilical cord blood. Methods Total of 200 pregnant women were studied and divided into two groups: water delivery group (n=100) and conventional delivery group(n=100). Humanumbilical cord blood was collected from both groups and analyzed in quality. Results The amount of collected cord blood, the number of nucleated cells, the rate of bacterial contamination，the activity and number of CD34+ hematopoietic stem cells from water delivery group were over to those from conventional delivery group. Conclusion Delivery style is an important factor that affects the quality of umbilical cord blood. Water delivery is an effective delivery style to improve the quality of cord blood and provide clinic with good quality of cord blood stem cell.【Key words】collection method of cord blood; water delivery; quality of cord blood自从1988年在巴黎为一位范可尼贫血患者施行了第一例脐带血造血干细胞移植手术以来，到2007年，全球造血干细胞移植1/4均来自脐带血。

免疫监控指导治疗脐血移植后急性移植物抗宿主病一例并文献复习

DOI： 10.3969/j.issn.0253-9802.2024.03.011研究论著免疫监控指导治疗脐血移植后急性移植物抗宿主病一例并文献复习吴正宙詹丽萍阙丽萍吴晓君徐宏贵黄科【摘要】目的探讨以皮疹为主要表现的急性移植物抗宿主病（aGVHD）在免疫监控指导下的精准诊疗效果。

方法回顾1例因高危急性髓系白血病接受非血缘脐血移植、其后出现顽固性皮疹的患儿的临床资料及诊治过程，以“造血干细胞移植”“免疫重建”和“急性移植物抗宿主病”的中英文为检索词，对以下数据库的相关论文进行检索：PubMed、Web of Science、CNKI、万方数据知识服务平台，收集检索到的病例资料并进行分析。

结果该例1岁9月龄女性患儿成功获得造血重建，移植后22 d患儿头部、后颈部出现密集红色丘疹（面积约19%）、瘙痒明显，口服他克莫司、外用激素类药物后皮疹无好转（面积>90%），此时监测CD3+细胞、CD8+细胞、CD3+CD69+细胞、CD3+HLA-DR+细胞比例明显升高，调节性T细胞（Treg）比例下降。

免疫指标支持T淋巴细胞活化，考虑Ⅱ度aGVHD（皮肤3级），遂予加强免疫抑制治疗方案。

期间患儿皮疹有消退，但仍反复，并伴皮肤明显脱屑，复查CD3+细胞、CD8+细胞、CD3+HLA-DR+细胞比例仍偏高，遂再次调整治疗方案、加强抗排斥，患儿皮肤aGVHD好转。

定期监测原发病完全缓解、植入比例100%、免疫重建稳定，随访至移植后32个月，患儿无病存活。

检索到相关文献9 篇，均论证了早期CD3+细胞、CD8+细胞、活化T淋巴细胞比例升高以及Treg下调与aGVHD发生相关。

结论对异基因造血干细胞移植术后患者的免疫状态进行动态监测，有利于评估其免疫重建情况及疾病状态（如aGVHD），有助于制定合理的免疫抑制治疗方案，使患者获得良好预后。

【关键词】造血干细胞移植；脐血；急性移植物抗宿主病；免疫监控；免疫重建Treatment of acute graft-versus-host disease after umbilical cord blood transplantation under the guidance of immune monitoring： a case report and literature review Wu Zhengzhou， Zhan Liping， Que Liping， Wu Xiaojun， Xu Honggui， Huang Ke. Department of Pediatrics， Sun Yat-sen Memorial Hospital， Sun Yat-sen University， Guangzhou 510120， China Corresponding author， Huang Ke， E-mail:*************【Abstract】Objective To explore the e ff ect of the precise diagnosis and treatment of a case of acute graft-versus-host disease （aGVHD） with rash as the main manifestation under the guidance of immune monitoring. Methods Clinical data and diagnosis and treatment of a child with high-risk acute myeloid leukemia who developed intractable rash after receiving unrelated umbilical cord blood transplantation were retrospectively analyzed. Using the keywords of “hematopoietic stem cell transplantation”“immune reconstitution” and “acute graft-versus-host disease”， relevant literatures were searched from PubMed， Web of Science， CNKI and Wanfang databases， and relevant cases were collected and analyzed. Results Hematopoietic reconstruction was successfully achieved in this 1 year 9 months old girl. On day 22 after transplantation， the skin of head and back neck of the patient showed dense red papules （approximately 19% in area）， with obvious itching. Under the treatment of oral tacrolimus and topical hormone drugs， the rash was not alleviated （area>90%）. Meanwhile， the proportion of CD3+ cells， CD8+ cells， CD3+CD69+cells and CD3+HLA-DR+cells was signi fi cantly increased， and the proportion of Treg cells was decreased. The immune indexes supported T lymphocyte activation， then diagnosis of gradeⅡaGVHD （skin grade 3） was made， and the immunosuppressive regimen was strengthened. During this period，the skin rash of the child subsided， but recurred， and accompanied by obvious skin desquamation. The proportion of CD3+ cells， CD8+ cells， and CD3+HLA-DR+cells remains relatively high， supporting the diagnosis of aGVHD. Therefore， the anti-GVHD regimen was adjusted and strengthened， and the aGVHD of the child’s skin was mitigated. Regular monitoring showed that the primary disease was completely relieved， the implantation rate was 100%， and the immune reconstitution was stable. During 32-month follow-up after transplantation， the child obtained disease-free survival. Nine relevant literatures were retrieved， all of which demonstrated that基金项目：广州市科技计划项目（2023A04J2085）作者单位：510120 广州，中山大学孙逸仙纪念医院儿科通信作者，黄科，E-mail:*************the increased proportion of early CD3+ cells ， CD8+ cells ， activated T lymphocytes and Treg downregulation were associated with the occurrence of aGVHD. Conclusion The dynamic monitoring of the immune status of patients after allogeneic hematopoietic stem cell transplantation is helpful to evaluate their immune reconstitution and disease status （such as ， aGVHD ）， thereby assisting clinicians to formulate a reasonable immunosuppressive regimen and bring favorable prognosis to patients.【Key words 】 Hematopoietic stem cell transplantation ； Cord blood ； Acute graft -versus -host disease ； Immune monitoring ；Immune reconstitution结果一、1例以顽固性皮疹为主要表现的aGVHD 患儿的病历资料1.一般情况患儿女，1岁9月龄，因“确诊AML 4月余，拟行造血干细胞移植术”于2019年8月19日入我院治疗。

丁香酚增强MSCs_对肝星状细胞炎性活化的抑制作用

第 44卷第5期2023 年9月Vol.44 No.5September 2023中山大学学报（医学科学版）JOURNAL OF SUN YAT⁃SEN UNIVERSITY（MEDICAL SCIENCES）丁香酚增强MSCs对肝星状细胞炎性活化的抑制作用吴逐宇1，汪显耀1，陈艳2，何志旭3（1. 遵义医科大学免疫学教研室，贵州遵义 563099； 2. 贵州省儿童医院 // 遵义医科大学附属医院小儿内科，贵州遵义 563099； 3. 遵义医科大学 // 教育部组织损伤与再生医学协同创新中心，贵州遵义 563099）摘要：【目的】　研究丁香酚对人脐带间充质干细胞（HUC-MSCs）抑制肝星状细胞（HSCs）炎性活化以及巨噬细胞促炎表型作用的影响及其相关机制；【方法】　体外培养并鉴定HUC-MSCs，并采用MTT法评估丁香酚对HUC-MSCs的毒力作用；体外划痕实验探究丁香酚对HUC-MSCs迁移能力的影响；丁香酚处理HUC-MSCs后的旁分泌产物（EU-MSCs-CM）、HUC-MSCs旁分泌产物（MSCs-CM）处理经TGF-β1处理活化的LX-2细胞，WB实验检测LX-2的α-SMA、COL1A1、Smad2/3、p-Smad2/3表达的变化。

EU-MSCs-CM、MSCs-CM处理经脂多糖（LPS）诱导的THP-1巨噬细胞，流式细胞术检测THP-1巨噬细胞表面标志物CD11b、CD86、CD206的表达情况，qPCR检测THP-1巨噬细胞促炎基因TNF-α、IL-1β、IL-6的表达情况。

【结果】　MTT法结果显示在0、7.5、15 µg/mL浓度处理细胞24 h、48 h 后，细胞活力保持在90%以上；体外划痕显示，丁香酚处理可以增强HUC-MSCs迁移能力。

WB结果显示，与MSCs-CM处理相比，EU-MSCs-CM处理对活化状态的HSCs的α-SMA、COL1A1、Smad2/3、p-Smad2/3表达抑制作用更加显著。

孕期母体补充益生菌及分娩方式对新生儿胆红素代谢的影响

孕期母体补充益生菌及分娩方式对新生儿胆红素代谢的影响李文平１，武红利２，曹迪２，张新颖２，梁淑新２，李瑞２（１．河北大学临床医学院，河北保定　０７１０００；２．河北大学附属医院产科，河北保定　０７１０００）［摘　要］目的：探讨孕期母体补充益生菌及分娩方式对新生儿胆红素代谢的影响。

方法：将符合入组标准的２００例孕妇均分为观察组（妊娠３２～３６周每日口服益生菌制剂１袋）及对照组（不予干预），再依据分娩方式分为剖宫产组与经阴道分娩组，收集各组孕妇一般资料［孕妇年龄、孕期增重、丙氨酸氨基转移酶（ＡＬＴ）、天冬氨酸氨基转移酶（ＡＳＴ）、血红蛋白（ＨＧＢ）含量及肌酐水平］及新生儿一般资料［新生儿性别比例、出生孕周、体质量、身长、阿氏（Ａｐｇａｒ）评分］、新生儿脐静脉血总胆红素（ＴＢ）、结合胆红素（ＣＢ）、未结合胆红素（ＵＣＢ），比较新生儿出生后２４、４８及７２ｈ的经皮胆红素（ＴＣＢ）值。

结果：各组孕妇及新生儿一般情况比较，差异无统计学意义（Ｐ＞００５）；观察组中剖宫产及经阴道分娩新生儿脐带血ＴＢ、ＵＣＢ均低于对照组相同分娩方式新生儿，差异有统计学意义（Ｐ＜００５）；观察组中剖宫产及经阴道分娩新生儿出生后２４、４８及７２ｈ的ＴＣＢ均低于对照组相同分娩方式新生儿，４８ｈ与７２ｈ的ＴＣＢ比较，差异有统计学意义（Ｐ＜００５）；２组新生儿中，经阴道分娩新生儿脐带血ＴＢ、ＵＣＢ及ＣＢ均低于剖宫产新生儿，但差异无统计学意义（Ｐ＞００５）；观察组经阴道分娩新生儿出生后２４、４８及７２ｈ的ＴＣＢ均低于本组剖宫产新生儿，４８ｈ与７２ｈ的ＴＣＢ比较，差异有统计学意义（Ｐ＜００５）；对照组经阴道分娩新生儿４８及７２ｈ的ＴＣＢ低于本组剖宫产新生儿，差异有统计学意义（Ｐ＜００５）。

结论：孕母妊娠３２～３６周补充益生菌制剂，可促进新生儿胆红素的代谢，降低新生儿胆红素水平；且经阴道分娩方式可以促进新生儿胆红素代谢。

［关键词］怀孕期间；婴儿，新生；胆红素；黄疸；肝功能；益生菌［中图分类号］Ｒ７１４［文献标识码］Ａ［文章编号］２０９６８３８８（２０２０）０８０９６７０６ＤＯＩ：１０．１９３６７／ｊ．ｃｎｋｉ．２０９６８３８８．２０２０．０８．０１９ＥｆｆｅｃｔｓｏｆＭａｔｅｒｎａｌＰｒｏｂｉｏｔｉｃＳｕｐｐｌｅｍｅｎｔａｔｉｏｎｄｕｒｉｎｇＰｒｅｇｎａｎｃｙａｎｄＤｅｌｉｖｅｒｙＭｏｄｅｏｎＢｉｌｉｒｕｂｉｎＭｅｔａｂｏｌｉｓｍｉｎＮｅｏｎａｔｅｓＬＩＷｅｎｐｉｎｇ１，ＷＵＨｏｎｇｌｉ２，ＣＡＯＤｉ２，ＺＨＡＮＧＸｉｎｙｉｎｇ２，ＬＩＡＮＧＳｈｕｘｉｎ２，ＬＩＲｕｉ２（１．ＳｃｈｏｏｌｏｆＣｌｉｎｃｌｉａｌＭｅｄｉｃｉｎｅ，ＨｅｂｅｉＵｎｉｖｅｒｓｉｔｙ，Ｂａｏｄｉｎｇ０７１０００，Ｈｅｂｅｉ，Ｃｈｉｎａ；２．ＤｅｐａｒｔｍｅｎｔｏｆＯｂｓｔｅｔｒｉｃｓ，ｔｈｅＡｆｆｉｌｉａｔｅｄＨｏｓｐｉｔａｌｏｆＨｅｂｅｉＵｎｉｖｅｒｓｉｔｙ，Ｂａｏｄｉｎｇ０７１０００，Ｈｅｂｅｉ，Ｃｈｉｎａ）［Ａｂｓｔｒａｃｔ］Ｏｂｊｅｃｔｉｖｅ：Ｔｏｉｎｖｅｓｔｉｇａｔｅｔｈｅｅｆｆｅｃｔｓｏｆｍａｔｅｒｎａｌｐｒｏｂｉｏｔｉｃｓｓｕｐｐｌｅｍｅｎｔａｔｉｏｎｄｕｒｉｎｇｐｒｅｇｎａｎｃｙａｎｄｄｅｌｉｖｅｒｙｍｏｄｅｏｎｂｉｌｉｒｕｂｉｎｍｅｔａｂｏｌｉｓｍｉｎｎｅｏｎａｔｅｓ．Ｍｅｔｈｏｄｓ：２００ｐｒｅｇｎａｎｔｗｏｍｅｎｗｈｏｍｅｔｔｈｅｉｎｃｌｕｓｉｏｎｃｒｉｔｅｒｉａｗｅｒｅｄｉｖｉｄｅｄｉｎｔｏａｎｏｂｓｅｒｖａｔｉｏｎｇｒｏｕｐ（ｄａｉｌｙｏｒａｌａｄｍｉｎｉｓｔｒａｔｉｏｎｏｆａｂａｇｏｆｐｒｏｂｉｏｔｉｃｓｆｏｒ３２ｔｏ３６ｗｅｅｋｓ＇ｇｅｓｔａｔｉｏｎ）ａｎｄａｃｏｎｔｒｏｌｇｒｏｕｐ（ｗｉｔｈｏｕｔｉｎｔｅｒｖｅｎｔｉｏｎ）．Ａｆｔｅｒｇｉｖｉｎｇｂｉｒｔｈ，ｔｈｅｙｗｅｒｅｄｉｖｉｄｅｄｉｎｔｏａｃｅｓａｒｅａｎｓｅｃｔｉｏｎｇｒｏｕｐａｎｄａｖａｇｉｎａｌｄｅｌｉｖｅｒｙｇｒｏｕｐａｃｃｏｒｄｉｎｇｔｏｔｈｅｄｅｌｉｖｅｒｙｍｏｄｅ．Ｇｅｎｅｒａｌｉｎｆｏｒｍａｔｉｏｎｏｆｐｒｅｇｎａｎｔｗｏｍｅｎｉｎｅａｃｈｇｒｏｕｐｓｕｃｈａｓａｇｅ，ｗｅｉｇｈｔｇａｉｎｄｕｒｉｎｇｐｒｅｇｎａｎｃｙ，ａｌａｎｉｎｅａｍｉｎｏｔｒａｎｓｆｅｒａｓｅ（ＡＬＴ），ａｓｐａｒｔａｔｅａｍｉｎｏｔｒａｎｓｆｅｒａｓｅ（ＡＳＴ），ｈｅｍｏｇｌｏｂｉｎ（ＨＧＢ）ａｎｄｃｒｅａｔｉｎｉｎｅａｓｗｅｌｌａｓｇｅｎｅｒａｌｉｎｆｏｒｍａｔｉｏｎｏｆｎｅｗｂｏｒｎｓｓｕｃｈａｓｓｅｘｒａｔｉｏａｔｂｉｒｔｈ，ｇｅｓｔａｔｉｏｎａｌａｇｅ，ｂｏｄｙｍａｓｓ，ｂｏｄｙｌｅｎｇｔｈ，ａｎｄＡｐｇａｒｓｃｏｒｅｗｅｒｅｃｏｌｌｅｃｔｅｄ．Ｔｈｅｔｏｔａｌｂｉｌｉｒｕｂｉｎ（ＴＢ），ｂｏｕｎｄｂｉｌｉｒｕｂｉｎ７６９第４５卷　第８期２０２０年８月贵州医科大学学报ＪＯＵＲＮＡＬＯＦＧＵＩＺＨＯＵＭＥＤＩＣＡＬＵＮＩＶＥＲＳＩＴＹＶｏｌ．４５　Ｎｏ．８２０２０．８［基金项目］河北省自然科学基金项目（Ｈ２０１８２０１１７９）通信作者Ｅｍａｉｌ：ｆｙｃｋｗｈｌ＠１６３．ｃｏｍ（ＣＢ）ａｎｄｕｎｂｏｕｎｄｂｉｌｉｒｕｂｉｎ（ＵＣＢ）ｉｎｕｍｂｉｌｉｃａｌｖｅｎｏｕｓｂｌｏｏｄｏｆｎｅｏｎａｔｅｓｗｅｒｅａｌｓｏｃｏｌｌｅｃｔｅｄ．Ｔｈｅｖａｌｕｅｓｏｆｔｒａｎｓｄｅｒｍａｌｂｉｌｉｒｕｂｉｎ（ＴＣＢ）ａｔ２４，４８ａｎｄ７２ｈａｆｔｅｒｂｉｒｔｈｗｅｒｅｃｏｍｐａｒｅｄ．Ｒｅｓｕｌｔｓ：Ｔｈｅｃｏｍｐａｒｉｓｏｎｏｆｇｅｎｅｒａｌｃｏｎｄｉｔｉｏｎｓｏｆｐｒｅｇｎａｎｔｗｏｍｅｎａｎｄｎｅｗｂｏｒｎｓｉｎｅａｃｈｇｒｏｕｐｓｈｏｗｅｄｎｏｓｔａｔｉｓｔｉｃａｌｌｙｓｉｇｎｉｆｉｃａｎｔｄｉｆｆｅｒｅｎｃｅ（Ｐ＞０．０５）；Ｉｎｔｈｅｏｂｓｅｒｖａｔｉｏｎｇｒｏｕｐ，ＴＢａｎｄＵＣＢｏｆｕｍｂｉｌｉｃａｌｃｏｒｄｂｌｏｏｄｏｆｎｅｗｂｏｒｎｓｄｅｌｉｖｅｒｅｄｂｙｃｅｓａｒｅａｎｓｅｃｔｉｏｎａｎｄｖａｇｉｎａｌｄｅｌｉｖｅｒｙｗｅｒｅｌｏｗｅｒｔｈａｎｔｈｏｓｅｉｎｔｈｅｃｏｎｔｒｏｌｇｒｏｕｐ，ａｎｄｔｈｅｄｉｆｆｅｒｅｎｃｅｓｗｅｒｅｓｔａｔｉｓｔｉｃａｌｌｙｓｉｇｎｉｆｉｃａｎｔ（Ｐ＜０．０５）．ＴＣＢａｔ２４，４８ａｎｄ７２ｈａｆｔｅｒｂｉｒｔｈｏｆｃｅｓａｒｅａｎｓｅｃｔｉｏｎａｎｄｔｒａｎｓｖａｇｉｎａｌｄｅｌｉｖｅｒｙｎｅｗｂｏｒｎｓｉｎｔｈｅｏｂｓｅｒｖａｔｉｏｎｇｒｏｕｐｗａｓｌｏｗｅｒｔｈａｎｔｈａｔｏｆｔｈｅｃｏｎｔｒｏｌｇｒｏｕｐ，ａｎｄＴＣＢａｔ４８ａｎｄ７２ｈａｆｔｅｒｂｉｒｔｈｓｈｏｗｅｄｓｔａｔｉｓｔｉｃａｌｌｙｓｉｇｎｉｆｉｃａｎｔｄｉｆｆｅｒｅｎｃｅ（Ｐ＜００５）；Ｉｎｔｈｅｔｗｏｇｒｏｕｐｓｏｆｎｅｗｂｏｒｎｓ，ＴＢ，ＵＣＢａｎｄＣＢｏｆｕｍｂｉｌｉｃａｌｃｏｒｄｂｌｏｏｄｏｆｎｅｗｂｏｒｎｓｄｅｌｉｖｅｒｅｄｔｈｒｏｕｇｈｖａｇｉｎａｗｅｒｅａｌｌｌｏｗｅｒｔｈａｎｔｈｏｓｅｄｅｌｉｖｅｒｅｄｂｙｃｅｓａｒｅａｎｓｅｃｔｉｏｎ，ｂｕｔｔｈｅｄｉｆｆｅｒｅｎｃｅｓｗｅｒｅｎｏｔｓｔａｔｉｓｔｉｃａｌｌｙｓｉｇｎｉｆｉｃａｎｔ（Ｐ＞０．０５）．ＴＣＢｏｆｎｅｗｂｏｒｎｓｄｅｌｉｖｅｒｅｄｔｈｒｏｕｇｈｖａｇｉｎａａｔ２４，４８ａｎｄ７２ｈａｆｔｅｒｂｉｒｔｈｉｎｔｈｅｏｂｓｅｒｖａｔｉｏｎｇｒｏｕｐｗａｓｌｏｗｅｒｔｈａｎｔｈａｔｏｆｎｅｗｂｏｒｎｓｄｅｌｉｖｅｒｅｄｔｈｒｏｕｇｈｃｅｓａｒｅａｎｓｅｃｔｉｏｎ，ａｎｄＴＣＢａｔ４８ａｎｄ７２ｈｏｆｌｉｆｅｗａｓｓｔａｔｉｓｔｉｃａｌｌｙｓｉｇｎｉｆｉｃａｎｔ（Ｐ＜０．０５）．Ｉｎｔｈｅｃｏｎｔｒｏｌｇｒｏｕｐ，ｔｈｅＴＣＢｏｆｎｅｗｂｏｒｎｓｄｅｌｉｖｅｒｅｄｂｙｖａｇｉｎａｆｏｒ４８ａｎｄ７２ｈｏｆｌｉｆｅｗａｓｌｏｗｅｒｔｈａｎｔｈａｔｏｆｔｈｅｎｅｗｂｏｒｎｓｄｅｌｉｖｅｒｅｄｂｙｃｅｓａｒｅａｎｓｅｃｔｉｏｎ，ａｎｄｔｈｅｒｅｗａｓａｓｔａｔｉｓｔｉｃａｌｌｙｓｉｇｎｉｆｉｃａｎｔｄｉｆｆｅｒｅｎｃｅ（Ｐ＜０．０５）．Ｃｏｎｃｌｕｓｉｏｎ：Ｐｒｏｂｉｏｔｉｃｓｓｕｐｐｌｅｍｅｎｔａｔｉｏｎａｔ３２ｔｏ３６ｗｅｅｋｓ＇ｇｅｓｔａｔｉｏｎｃａｎｐｒｏｍｏｔｅｔｈｅｍｅｔａｂｏｌｉｓｍｏｆｂｉｌｉｒｕｂｉｎｉｎｎｅｏｎａｔｅｓａｎｄｒｅｄｕｃｅｔｈｅｂｉｌｉｒｕｂｉｎｌｅｖｅｌ．Ｔｒａｎｓｖａｇｉｎａｌｄｅｌｉｖｅｒｙｃａｎｂｏｏｓｔｔｈｅｍｅｔａｂｏｌｉｓｍｏｆｂｉｌｉｒｕｂｉｎｉｎｎｅｏｎａｔｅｓ．［Ｋｅｙｗｏｒｄｓ］ｐｅｒｉｐａｒｔｕｍｐｅｒｉｏｄ；ｉｎｆａｎｔ，ｎｅｗｂｏｒｎ；ｂｉｌｉｒｕｂｉｎ；ｊａｕｎｄｉｃｅ；ｌｉｖｅｒｆｕｃｔｉｏｎ；ｐｒｏｂｉｏｔｉｃｓ新生儿黄疸是新生儿疾病中的常见病，分为生理性和病理性，生理性黄疸虽可自行消退，但长时间较高浓度的胆红素水平也会对新生儿的智力发育产生影响［１］。

脐血间充质干细胞移植脊髓损伤恢复期患者的免疫功能变化

中国组织工程研究第16卷第1期 2012–01–01出版Chinese Journal of Tissue Engineering Research January 1, 2012 Vol.16, No.1 ISSN 1673-8225 CN 21-1581/R CODEN: ZLKHAH171 1Department of Rehabilitation Medicine, Shenzhen Nanshan Affiliated Hospital of Guangdong Medical University, Shenzhen 518052, Guangdong Province, China;2Department of Rehabilitation Medicine, Longgang Central Hospital of Shenzhen, Shenzhen 518000, Guangdong Province, China Zhao Ning★, Master, Attending physician, Department of Rehabilitation Medicine, Shenzhen Nanshan Affiliated Hospital of Guangdong Medical University, Shenzhen 518052, Guangdong Province, China zhloo7692@yahoo. Correspondence to: Yang Wan-zhang, Doctor, Professor, Chief physician, Master’s supervisor, Department of rehabilitation medicine, Shenzhen Nanshan Affiliated Hospital of Guangdong Medical University, Shenzhen 518052, Guangdong Province, China Supported by: Foundation of Science and Technology Bureau of Shenzhen, No. 200405219* Received: 2011-07-28 Accepted: 2011-09-15脐血间充质干细胞移植脊髓损伤恢复期患者的免疫功能变化*★赵宁1，杨万章1，张敏1，盛佑祥1，唐映2Effect of umbilical cord blood mesenchymal stem cells transplantation on immune function of spinal cord injury patientsZhao Ning1, Yang Wan-zhang1, Zhang Min1, Sheng You-xiang1, Tang Ying2AbstractBACKGROUND: Mesenchymal stem cells (MSCs) transplantation has certain immunogenicity. However, there was no in-depthand system reports about the effect of MSCs transplantation on spinal cord injury patient’s immune function.OBJECTIVE: To observe the effects of umbilical cord blood MSCs (UCB-MSCs) transplantation on immune function of spinalcord injury (SCI) patients.METHODS: Sixty-one spinal cord injury (SCI) patients were treated by intervenous drop infusion and lumbar subarachnoid spacepuncture to UCB-MSCs. Flow-cytometry and immunoturbidimetry (ITM) were used to detect the content of T lymphocyticsubgroup and immune globulin and the alexinic in plasma of all the patients prior and post-transplantation.RESULT AND CONXLUSION: Compare with pre-transplantation, the content of CD3+, IgA and IgG was decreased and thedifferences were significant; The content of CD4+, CD8+, CD4+/CD8+, IgM, C3 and C4 was also changed and the differences werenot significant. It indicates that UCB-MSCs transplantation could not activate the immunological response of cell and humoralimmunity, so the transplantation is safety. Negative accommodation is exist, but the relevant indicators need a furtherimprovement, expand samples, and clearly relevant mechanism.Zhao N, Yang WZ, Zhang M, Sheng YX, Tang Y.Effect of umbilical cord blood mesenchymal stem cells transplantation onimmune function of spinal cord injury patients.Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(1): 171-174.[ ]摘要背景：间充质干细胞移植因其具有一定的免疫原性，对脊髓损伤患者免疫功能的影响尚无深入系统的报道。

新生儿脐带血血气指标分析在预后评估中的应用进展

1 新生儿脐带血血气分析的操作与意义
脐带血血气分析指的是胎儿分娩出以后，快速的采集脐带以及检测血气，临床上主要用来分娩出但是无法建立自主呼吸的胎儿中，在直视的情况下，在胎儿的一侧长度大约为 10 厘米的脐带处，用两把消毒止血钳钳夹主外侧脐带，并剪断；再应用肝素化注射器对脐动脉或静脉血进行穿刺，密闭封好后送检，得出血气分析值 [3]。一般正常情况下，胎儿体内的酸碱处于平衡状态；胎儿若是处于缺氧状态的话，胎盘和血管内可能出现缓冲的情况，从而产生过度的① CO2、②乳酸堆积，直至酸碱平衡失去代偿能力，进而表现出酸中毒的现象。脐动脉的血流是从胎儿流出到胎盘绒毛间隙，可以有效的将胎儿具体情况反映出来；脐静脉血流主要是从胎盘流向胎儿，可以有效的将产妇的酸碱平衡与胎盘功能反映出来 [4]。因此，可以通过检测动脉血气分析值，监测新生儿是否有窒息现象
存在，以及其他不良预后情况存在。
2 新生儿脐带血血气分析正常范围参考值
因研究对象不同，再加上脐带血气分值参数容易受到诸多因素的影响，例如①胎龄、②分娩方式等，导致各参数正常值，目前未能形成统一的参考范围。以往有研究数据显示：正常新生儿脐带血血气分析正常值的测值范围为：①动脉血 SO2 平均值为：24.3%；② PH 平均值为：7.25；③ BE 值为：-4.3mol； ④ PO2 值为：16mmHg；⑤ PCO2 值为：50mmHg[5]。新生儿脐动脉血气指标研究写作组，经期研究脐动脉血 pH 值与 BE 值的统计学参考范围分别为 (7.20±0.20) 与 (-7.64±10.02)；新生儿窒息脐动脉血，pH 临床校正正常范围约为 >7.00~>7.20， BE 分布范围为 >-10~>-18[6]。

脐带血干细胞治疗心梗后心源性休克合并重度水肿1例

脐带血干细胞治疗心梗后心源性休克合并重度水肿1例林萍;周鑫【期刊名称】《当代医学》【年(卷),期】2014(000)022【总页数】2页(P26-26,27)【关键词】脐带血干细胞;心源性休克【作者】林萍;周鑫【作者单位】辽宁 110032 辽宁中医药大学附属医院;辽宁 110032 辽宁中医药大学附属医院【正文语种】中文干细胞移植是目前新兴的一种治疗方法，也是一种前景乐观的治疗方法。

人脐血干细胞（HUCBC）移植则是干细胞移植中的热点。

虽然相关研究多为基础研究[1-7]，但临床效果也不容忽视。

1.1 入院时情况患者男性，56岁，因“反复发作胸闷、胸痛7个月加”于2013年8月5日入院。

患者7个月前劳累后出现胸闷、胸痛，呈持续性，就诊于“沈阳军区总医院”，诊断为“急性下壁心肌梗死”，予以急诊行冠状动脉造影检查示：左主干(LM)（-），左前降支（LAD）近中段多处狭窄50%～60%，冠状动脉血流（TIMI血流）3级，回旋支(LCX)近中段狭窄70%，TIMI血流3级，右冠状动脉(RCA)近段80%狭窄，中段100%闭塞，TIMI血流0级，右优势型。

干预RCA，于RCA置入2枚支架，术中患者出现持续性血压下降，最低至80/60mmHg，予以升压药对症治疗（0.9%生理盐水500mL，多巴胺40mg，阿拉明40mg25mL/ h静脉泵入），并予以IABP支持治疗。

术后患者胸闷、胸痛症状缓解，病情好转出院。

出院后一直系统内科药物治疗。

1个月前无明显诱因再次出现胸闷、气短症状，伴有周身水肿、夜间不能平卧，遂来本院就诊，门诊以“冠心病、心力衰竭”收入院。

既往史：有糖尿病病史5年，自行应用胰岛素治疗，血糖控制欠佳。

入院查体：血压98/55mmHg，心率109次/min，双肺可闻及湿性啰音及少量干鸣音，心界向两侧扩大，心音低钝，律齐，双侧上、下肢及阴囊水肿明显。

辅助检查：心电图示：Ⅱ、Ⅲ、AVF 可见病理性Q波，伴有ST段抬高0.1～0.2mV。

Umbilical Cord Blood An Alternate Source of Stem Cells

Drawbacks of UCSC

Longer time to immune system recovery due to immaturity of cells Leads to increased GVHD and infection risk (but diseases less severe than with BM) Less effective for adults Cell dose of one sample usually inadequate for patients with increased body mass Possible unknown genetic conditions of newborn donor

Your Chances of Finding a Donor

10 million registered BM donors worldwide Caucasion has a 50% chance of finding a donor Minorities are lower, donor programs targeting subpopulations to increase diversity of donor pool. Basically, <50% chance of finding a donor under any circumstance.
Bone Marrow Stem Cells

Standard stem cell source for over 40 years Obtained by bone marrow aspiration of the pelvis of donor Relatively no risk to donor other than pain (no anesthetics used) From beginning search to actual transplant takes 3-5 months

程控硬膜外间歇脉冲注入模式在分娩镇痛中的应用及其对脐血流及脐动脉血气的影响

第47卷第5期2021年9月吉林大学学报（医学版）Journal of Jilin University（Medicine Edition）Vol.47No.5Sep.2021DOI：10.13481/j.1671‑587X.20210525程控硬膜外间歇脉冲注入模式在分娩镇痛中的应用及其对脐血流及脐动脉血气的影响潘雪琳1,刘庆2,张英2,唐勇1（1.四川锦欣妇女儿童医院麻醉科，四川成都610000；2.西南医科大学附属中医医院麻醉科，四川泸州646699）［摘要］目的目的：探讨程控硬膜外间歇脉冲注入（PIEB）模式用于分娩镇痛的效果，以及对脐血流及脐动脉血气的影响，为临床寻找更为理想的硬膜外分娩镇痛给药模式提供依据。

方法方法：选择要求行分娩镇痛的足月单胎初产妇90例陆续入组观察，采用随机数字表法将研究对象产妇随机分为PIEB组和持续性硬膜外输注（CEI）组，每组45例。

2组产妇均给予首次剂量（0.08g·L－1罗哌卡因+0.4mg·L－1右美托咪定）10mL。

PIEB组产妇注入首次剂量后1h连接PIEB镇痛泵，脉冲剂量8mL·h－1，速度7mL·min－1。

CEI组产妇注入首次剂量后立即连接CEI镇痛泵，持续背景剂量8mL·h－1。

比较2组产妇镇痛前（T0）、镇痛后30min（T1）、镇痛后1h（T2）、镇痛后2h（T3）和宫口开全时（T4）的视觉模拟评分（VAS）。

比较2组T0~T3的胎儿脐动脉波动指数（PI）、脐动脉阻力指数（RI）和脐动脉血流速度收缩峰值与舒张峰值比值（S/D）；比较2组胎儿娩出即刻的脐动脉血气pH值、剩余碱（BE）值和新生儿Apgar评分；比较2组产妇第一产程时间、第二产程时间、满意度和不良反应。

结果结果：2组产妇在T0、T1、T2和T3的VAS评分比较差异无统计学意义（P>0.05）；PIEB组T4时间点的VAS评分低于CEI组（P<0.05）。

脐带间充质干细胞移植治疗初发1型糖尿病_于文龙

中国组织工程研究与临床康复第15卷第23期 2011–06–04出版Journal of Clinical Rehabilitative Tissue Engineering Research June 4, 2011 Vol.15, No.23 ISSN 1673-8225 CN 21-1539/R CODEN: ZLKHAH4363 1Department of Endocrine,2Chinese-American Research Center for Stem Cells, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, ChinaYu Wen-long★, Studying for master’s degree, Department of Endocrine, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China wenlongyuwenlong@ Correspondence to: Wang Yan-gang, Doctor, Chief physician, Doctoral supervisor, Department of Endocrine,Chinese-American Research Center for Stem Cells, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China wangyg1966@ Received: 2010-03-19 Accepted: 2010-05-24脐带间充质干细胞移植治疗初发1型糖尿病★于文龙1，高宏2，余霄龙1，王丽2，阎胜利1，王颜刚1，2Umbilical cord mesenchymal stem cells transplantation for newly-onset type 1 diabetes Yu Wen-long1, Gao Hong2, Yu Xiao-long1, Wang Li2, Yan Sheng-li1, Wang Yan-gang1,2AbstractBACKGROUND: Autologous hematopoietic stem cells transplantation can enhance function of beta cells of islet and prolongtime independent of insulin.OBJECTIVE: To observe the clinical efficacy of human umbilical cord mesenchymal stem cells (hUCMSCs) transplantation intreatment of newly-onset type 1 diabetes.METHODS: A total of 12 patients with newly-onset type 1 diabetes mellitus (less than 3 months) were selected. Six cases weretreated by hUCMSCs transplantation and insulin, and the other 6 were treated by insulin alone. Before and 9 months after transplantation, fasting and postprandial blood glucose, insulin dosage, C peptide, HbA1c changes and adverse events were measured.RESULTS AND CONCLUSION: Patients were followed up for 9 months. Fasting blood glucose, HbA1c and C peptide levelswere significantly improved in stem cell therapy group compared with control group. In addition to significantly reduced C peptide levels, other indexes remained unchanged in control group. Results showed that hUCMSCs transplantation has positive effectson treatment of newly-onset type l diabetes.Yu WL, Gao H, Yu XL, Wang L, Yan SL, Wang YG.Human umbilical cord mesenchymal stem cells transplantation fornewly-onset type 1 diabetes.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2011;15(23): 4363-4366.[ ]摘要背景：研究证实，新诊断的1型糖尿病患者，接受自体造血干细胞治疗后，绝大多数患者胰岛β细胞功能增强，不依赖胰岛素的时间也延长。