Antiviral activities of flavonoids and organic acid from Trollius chinensis Bunge

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Journal of Ethnopharmacology79(2002)365–368

Antiviral activities ofﬂavonoids and organic acid from Trollius

chinensis Bunge

Yao-Lan Li a,b,Shuang-Cheng Ma b,Yi-Ting Yang a,Shao-Ming Ye a,

Paul Pui-Hay But b,*

a Department of Chemistry,Jinan Uni6ersity,Guangzhou,People’s Republic of China

b Department of Biology and Institute of Chinese Medicine,The Chinese Uni6ersity of Hong Kong,Shatin,N.T.,Hong Kong

Received23July2001;received in revised form30October2001;accepted23November2001

Abstract

Theﬂower of Trollius chinensis Bunge is used for treating upper respiratory infections,pharyngitis,tonsillitis,and bronchitis in Chinese folk medicine.The antiviral activities of the crude extract,totalﬂavonoids,orientin,vitexin and proglobeﬂowery acid isolated from theﬂowers of T.chinensis against parainﬂuenza type3(Para3)virus were investigated.The results showed that the crude extract and totalﬂavonoids exhibited a weak antiviral activity against Para3.Orientin and vitexin demonstrated potent or moderate antiviral activity against Para3.Proglobeﬂowery acid showed weak antiviral activity against Para3.©2002Elsevier Science Ireland Ltd.All rights reserved.

Keywords:Trollius chinensis Bunge;Cytotoxicity;Antiviral activity;Para3

/locate/jethpharm

1.Introduction

Trollius chinensis Bunge(Ranunculaceae)is an en-demic growing widely in northern China.Itsﬂowers are often used to treat upper respiratory infections,pharyn-gitis,tonsillitis,and bronchitis in Chinese folk medicine (Beijing Pharmacy Factory,1978).Previous studies in-dicated antibacterial activities in the crude aqueous extract of theﬂowers(Beijing Pharmacy Factory,1978; Bai,1994).These activities were ascribed to the ﬂavonoids present in the plant of Trollius species(Su et al.,1996;Yang et al.,2000).The crude aqueous extract of theﬂowers was also tested for antiviral activities against adenovirus,coxsackie,and ECHO virus,but the antiviral activities were found only against cox-sackie B3(Wen et al.,1999).

Until now,only a few compounds have been re-ported from this herb,including orientin,vitexin,3,4-dimethoxybenzamide,palmitic acid,veratric acid and volatile oil(Kang et al.,1984;Li,1982;Feng,1998). Clinical studies in the treatment of upper respiratory infections showed that the combined efﬁcacy is92.7% (Beijing Pharmacy Factory,1978).Upper respiratory infection,especially in children,is a common disease worldwide.Parainﬂuenza type3(Para3)virus has been recognized as a major cause of such infections(Couch, 1997).No chemotherapy agents and vaccine have been used to treat Para3infections.Therefore,the develop-ment of new antiviral agents is most desirable.In this paper,we report the extraction,isolation and identiﬁca-tion ofﬂavonoids and organic acid from theﬂowers of T.chinensis,and their antiviral activities against Para3.

2.Materials and methods

2.1.Plant material

Theﬂowers of T.chinensis Bunge were collected from Hebei Province,People’s Republic of China,in1999.It has been authenticated by a voucher specimen (20012422)kept at the Museum,Institute of Chinese Medicine,The Chinese University of Hong Kong.

*Corresponding author.Tel.:+852-2609-6140;fax:+852-2603-

5248.

E-mail address:paulbut@.hk(P.P.-H.But).