贺宪益气补肾活血法防治家兔膝骨性关节炎的实验研究

益气补肾活血法防治家兔膝骨性关节炎的实验研究
益气补肾活血法防治家兔膝骨性关节炎的实验研究
中文摘要
目的：观察益气补肾行血方（北芪、牛膝、石斛、芍药、狗脊、红花、骨碎补、 青风藤）对家兔膝骨性关节炎模型的治疗作用及其可能的作用机制。
方法：将 60 只新西兰大耳兔随机分成四组，A 组 9 只，B～D 组各 17 只，采用 左后肢过伸位石膏管型固定法复制骨关节炎（Osteoarthritis,OA）动物模型。造 模一周后，A、B 组用生理盐水 8ml/天灌胃，C 组用壮骨关节丸 1.2g/天灌胃，D 组 用益气补肾行血方 15.6g/天灌胃。分别于灌胃后第 7 周和第 13 周，各组随机挑选 一半处死，各组关节进行大体观察和手术显微镜下观察，参照关节镜下标准进行分 级，取股骨髁负重区软骨进行组织病理学观察并做各组 C.Colombo 评分比较；用免 疫组织化学方法检测各组软骨细胞增殖情况，同时用末端原位标记法检测各组软骨 细胞凋亡情况。
Results: The finding of histological study showed that there were more or less degenerative changes of OA in group B～D. No degenerative changes occurred in group A. Surgical microscopic results of cartilage surface
结果：灌胃后第 7 周和第 13 周，B～D 组大体及镜下观察均有不同程度的 OA 软骨退变，A 组正常。在手术显微镜下 A～D 组关节软骨表面分级比较有统计学意 义（第 7 周，P＝0.001；第 13 周,P＝0.000）。D 组和 C 组病理总积分均明显低于 B 组（P<0.01），D 组低于 C 组（第 7 周，P＝0.004；第 13 周,P＝0.002）。灌胃后第 7 周，在增殖阳性细胞指数上， C、D 组高于 B 组（P<0.01）, D 组高于 C 组（P＝ 0.000）；在凋亡阳性细胞指数上，C、D 组低于 B 组（P<0.01），C 组与 D 组无明显 差异（P=0.567）。灌胃后第 13 周，在增殖阳性细胞指数上， B、C、D 各组之间均 无统计学差异（P >0.05）；在凋亡阳性细胞指数上，C、D 组低于 B 组（P<0.01）， D 组低于 C 组（P＝0.025）。
关键词：益气补肾活血法 骨性关节炎 关节软骨 细胞增殖 细胞凋亡 中医内治
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益气补肾活血法防治家兔膝骨性关节炎的实验研究
Experimental Studies on Preventing and Treatment of Knee
Osteoarthritis in Rabbit Models By Supplementing Qi, Tonifying Kidney
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益气补肾活血法防治家兔膝骨性关节炎的实验研究
英文缩略词
英文缩写 英文全称
中文全称
OA
osteoarthritis
骨性关节炎
ቤተ መጻሕፍቲ ባይዱ
MMPs
matrix metalloproteinase
基质金属蛋白酶
Methods : 60 New Zealand rabbits were randomly divided into four groups. Group A had 9 rabbits ,and each of group B～D had 17 ones. All animals in group B～D were managed by the plaster immobilization with left posterior knee joint in extend excessive position. One weeks later , group D were treated with YQBSXX recipe 15.6g once daily, group C with Zhuangguguanjie recipe 1.2g(a standard herbal prescription for OA in China), group A～B were received 8 ml normal saline. Half of each group were decapitated in the 7th weeks and the 13th weeks respectively. The gross morphological condition was observed and grade of cartilage surface was evaluated with surgical microscope according to arthroscope grade method. Specimens were taken from the weight-bearing portion of the femoral condylar and were examined histologically and graded with C.Colombo criteria. Immunohistochemical method was used to detect the proliferation of chondrocytes in all groups. At the same time , terminal-deoxynocleotidyl transferase mediated nick end labeling method(TUNEL) was used to detect the apoptosis of chondrocytes.
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益气补肾活血法防治家兔膝骨性关节炎的实验研究
showed significant difference among group A～D(P=0.001 in the 7th week and P=0.000 in the 13th week). In the 7th week and 13th week after consumption , according to C.Colombo score criterion, the average scores in group C～ D were lower than group B （P<0.01）, and group D than group C(P=0.004 in the 7th week and P=0.002 in the 13th week). In the 7th week after consumption , the chondrocytes proliferative index in group C～D were obviously higher group B（P<0.01） , and group D than group C（P=0.000）by immunohistochemical method; the chondrocytes apoptosis index in group C～D were obviously lower than group B（P<0.01）by TUNEL assay , but there was no obvious difference between group D and group C（P=0.567）. In the 13th week after consumption, there were no obvious difference in the chondrocytes proliferative index in group B～D, but the chondrocytes apoptosis index in group C～D were obviously lower than group B（P<0.01） , and group D than group C（P=0.025）.
Keywords: supplementing qi, tonifying kidney and promoting blood movement, osteoarthritis , articular cartilage, chondrocytes proliferation, chondrocytes apoptosis, internal treatment of TCM
and Promoting Blood Movement Abstract
Objiective: To investigate the effects and possible mechanisms of a new herbal recipe, yiqibushenxingxue recipe (YQBSXX) (Radix Astragali, Radix Achyranthis Bidentatae , Herba Dendrabii, Radix Paeoniae Alba, Rhizoma Cibotii, Flos Carthami, Rhizoma Drynariae, Caulis Sinomenii) on osteoarthritis (OA) in the knee joint in rabbit models.
Results: The method of plaster immobilization with the knee joint in extend excessive position can successfully induce the model of OA. The treatment of OA in a rabbit model with YQBSXX recipe shows significantly better histologic results , it can restrain effectively chondrocytes apoptosis and promote chondrocytes proliferation. To the mechanisms of treatment by supplementing qi, tonifying kidney and promoting blood movement, both restraining chondrocytes apoptosis and promoting proliferation probably have great meanings in the early/middle stage , but restraining chondrocytes apoptosis probably plays a main role in middle/late stage. Therefore, it is a useful agent for prevention and treatment of OA.Further research is needed, to assess side effects and to reveal the excact mechanisms of action of this Chinese herbal prescription.