Bioruptor_UCD-200超声波破碎仪说明书

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bioruptor pico使用手册

bioruptor pico使用手册Bioruptor Pico使用手册第一章：产品概述1.1 产品介绍Bioruptor Pico是一种高性能的超声波细胞破碎仪，用于破碎生物样品，提取DNA、RNA和蛋白质等目标分子。

它采用超声波震荡原理，能够快速、高效地破碎生物细胞，同时保持目标分子的完整性和活性。

1.2 主要特点- 高效破碎：Bioruptor Pico能够以超声波频率震动细胞，使得细胞壁破碎，释放内部的目标分子。

- 稳定性：仪器设计紧凑、结构稳定，可长时间连续运行，保证了实验的稳定性。

- 可控性：Bioruptor Pico提供多种工作模式和参数设置，以满足不同实验需求。

- 易于操作：仪器采用触摸屏和用户友好的操作界面，操作简便。

1.3 技术参数- 最大功率输出：500 W- 频率范围：10 kHz - 1 MHz- 破碎时间范围：1 s - 10 min- 体积：300 mm × 200 mm × 200 mm- 重量：5 kg第二章：安装与启动2.1 安装- 将Bioruptor Pico放置在水平平整的台面上，保证其稳定。

- 连接电源线，接通电源。

- 根据实验需要，选择合适的破碎器皿，将样品放入破碎器皿中。

2.2 启动- 使用手指点击仪器触摸屏上的电源按钮，启动仪器。

- 在仪器启动完成后，进入主界面。

- 在界面上选择所需的工作模式和工作参数，点击确认按钮即可开始实验。

第三章：使用方法3.1 操作界面- 在主界面上，显示当前的操作模式和参数设置。

- 点击“模式设置”按钮，可以选择不同的工作模式，如常规模式、高能模式等。

- 点击“参数设置”按钮，可以调整破碎时间、功率等参数。

3.2 样品处理- 将待处理样品放入破碎器皿中，确保样品的浓度和体积适宜。

- 盖上破碎器皿的盖子，确保样品不会溅出。

- 将装有样品的破碎器皿放入试样架中。

3.3 实验操作- 在主界面上选择工作模式和参数设置。

超声波使用说明书

N&DN系列(LCD)超声波细胞粉碎机使用说明书宁波新芝生物科技股份有限公司地址：宁波市国家高新技术园区木槿路65号 315013电话：7传真：3网址：一. 概述随着生物产业的发展，应用超声波细胞粉碎机所做的实验要求也随之提高，如对样品温度的测定、控制，低温冷却样品及整机的智能化程度的提高等等，都提出了新的要求，为进一步完善此类仪器的各项性能，我公司在现有各种型号的超声波细胞粉碎机的基础上，吸收国外最新技术，结合微电脑控制、选频、测温、保护等软硬件技术而研制的超声波细胞粉碎机，它具有技术先进、性能可靠、操作简便、外型美观、显示清晰明亮、测温控温精确等优点。

超声波细胞粉碎机是一种利用强超声在液体中产生空化效应，对物质进行超声处理的多功能、多用途的仪器，能用于动植物组织、细胞、细菌、芽胞菌种的破碎，同时可用来乳化、分离、分散、匀化、提取、脱气、清洗及加速化学反应等等。

该机广泛应用于生物化学、微生物学、药物化学、表面化学、物理学、动物学、农学、医学、制药等领域教学、科研、生产。

Ф12 1/2”20-25KHz 100-950W 100 - 200mlФ15 5/8”20-25KHz- 500mlФ18 3/4”20-25KHz 200-950W 200 - 500mlФ20 3/4” -1000mlФ25 1” -1200ml本机由超声波发生器和超声波换能器组件两大部分组成。

超声波发生器（电源）是将220VAC、50Hz的单相电通过变频器件变为20-25kHz、约600V的交变电能、并以适当的阻抗与功率匹配来推动换能器，作纵向机械振动，振动波通过浸入在样品溶液中的钛合金变幅杆对被破碎的各类细胞产生空化效应，从而达到破碎细胞之目的。

其电原理由整流电源，开关电源、变频系统、功率放大器、锁相频率自动跟踪器、功率调节器、功率检测器、功率保护器及微电脑控制等组成。

换能器组件是由压电振子、变幅放大器所组成的产生机械能的聚能变幅装置。

超声波破碎仪使用说明书

USER’S GUIDEAUTOTUNE SERIESHIGH INTENSITY ULTRASONIC PROCESSORMICROPROCESSOR CONTROLLED100 Watt Model • 130 Watt ModelTABLE OF CONTENTSWarrantyImportant SafeguardsLow Surface Tension Liquids - Organic SolventsSECTION 1 – INSTALLATIONInspectionElectrical RequirementsInstalling the Ultrasonic ProcessorSECTION II – OPERATIONPrinciples of Ultrasonic DisruptionFunctions of Controls, Indicators, and ConnectorsPreparations for UseUsing the Ultrasonic ProcessorSECTION III – SERVICE INFORMATIONOverload ConditionReturn of EquipmentSECTION IV – OPERATING SUGGESTIONS AND TECHNIQUESThe Ultrasonic Processor supplied with this instruction manual is constructed of the finest material and the workmanship meets the highest standards. It has been thoroughly tested and inspected before leaving the factory and when used in accordance with the procedures outlined in this manual, will provide you with many years of safe and dependable service.Rev. April 2004IMPORTANT SAFEGUARDSREAD BEFORE INSTALLING ORUSING THE EQUIPMENTYour Ultrasonic Processor has been designed with safety in mind. However, no design can completely protect against improper usage, which may result in bodily injury and/or property damage. For your protection and equipment safeguard, observe the following warnings at all times, read the operating instructions carefully before operating the equipment, and retain this instruction manual for future reference. If the ultrasonic Processor is used in a manner contrary to that specified in this instruction manual, the protection features designed into the unit may be impaired.• When mounting the probe, always clamp the converter housing. Never clamp the probe.• Make sure the Ultrasonic Processor is properly grounded via a 3-prong outlet.• High voltage is present in the power supply. Do not remove the cover. Refer all servicing to qualified service personnel.• To avoid electric shock, disconnect the electrical power cord before removing the cover prior to servicing.• Never operate the power supply unless it is connected to the converter.• Never secure anything to the probe, except at the nodal point (point of no activity).• Never touch a vibrating probe.• Never allow a microtip to vibrate in air for more than 10 seconds.• Never operate a threaded probe without a replaceable tip.• Air-cool the converter when sample temperature exceeds 100° C, and when working at high intensity for more than 30 minutes.• It is recommended that a sound abating enclosure or ear protection be used when operating the Ultrasonic Processor.CAUTIONLOW SURFACE TENSION LIQUIDS – ORGANIC SOLVENTS The probes (solid or with a replaceable tip) are tuned elements that resonate at a specific frequency. When using a ½” (13 mm) probe with a replaceable tip, if the replaceable tip is removed or isolated from the rest of the probe, the probe will no longer resonate at that frequency, and the power supply will fail.Unlike aqueous (water based) solutions, which rarely cause problems, samples containing solvents or low surface tension liquids are problematic. These liquids penetrate the probe/replaceable tip interface, and force the particulates into the threaded section isolating the tip from the probe.When processing samples containing solvents or low surface tension liquids ALWAYS use a solid probeSECTION 1 – INSTALLATIONINSPECTIONPrior to installing the Ultrasonic Processor, perform a visual inspection to detect any evidence of damage, which might have occurred during shipment. Before disposing of any packaging material, check it carefully for small items.The Ultrasonic Processor was carefully packed and thoroughly inspected before leaving our factory. The carrier, upon acceptance of the shipment, assumed responsibility for its safe delivery. Claims for loss or damage sustained in transit must be submitted to the carrier.If damage has occurred, contact your carrier within 48 hours of the delivery date. DO NOT OPERATE DAMAGED EQUIPMENT. Retain all packing materials for future shipment.ELECTRICAL REQUIREMENTSThe Ultrasonic Processor requires a fused, single phrase 3-terminal grounding type electrical outlet capable of supplying 50/60 Hz at 100 volts, 115 volts, 220 volts, or 240 volts, depending on the voltage option selected. For power requirements, check the label on the back of the unit.Should it become necessary to convert the unit for different voltage operation, proceed as follows.1. Ensure that the power cord is not connected to the electrical outlet.2. Open the fuse holder cover using a small screwdriver.3. Pull out the red fuse holder from its housing.4. To convert from 100/115V to 220/240V replace the two 3 Amp slow blow fuses,with 1.6 Amp fuses.5. To convert from 220/240V to 100/115V reverse the procedure above.6. Rotate the fuse holder 180º from its original position, and reinsert it into itshousing. For 100/115V operation the voltage displayed should be 115. For220/240V operation the voltage display should be 220.7. Change the electrical power cord as required.The Ultrasonic Processor should be installed in an area that is free from excessive dust,dirt, explosive and corrosive fumes, and extremes of temperature and humidity.SECTION II – OPERATIONPRINCIPLES OF ULTRASONIC DISRUPTIONThe ultrasonic power supply converts 50/60 Hz line voltage to high frequency electrical energy. This high frequency electrical energy is transmitted to the piezoelectric transducer within the converter, where it is changed to mechanical vibrations. The vibrations from the converter are intensified by the probe, creating pressure waves in the liquid. This action forms millions of microscopic bubbles (cavities), which expand during the negative pressure excursion, and implode violently during the positive excursion. As the bubbles implode, they cause millions of shock waves and eddies to radiate outwardly from the site of collapse, as well as generate extremes in pressures and temperatures at the implosion sites. Although this phenomenon, known as cavitation, lasts but a few microseconds, and the amount of energy released by each individual bubble is minimal, the cumulative amount of energy generated is extremely high. The larger the probe tip, the larger the volume that can be processed but at a lesser intensity. For information regarding the processing capability of each probe, consult the tables below.FUNCTIONS OF KEYS, CONTROLS, INDICATORS, AND CONNECTORS (cont.)REAR PANEL9 pin D-sub connector Connects to external actuation device, and enable power and frequency monitoring.Footswitch jack Connects to the footswitch cable.Coax connector Connects to the converter.Power module Connects to the electrical line cord and encases the fuse(s).9-PIN D-SUB CONNECTORPin No. Description1 Notconnected2 Notconnected3 Notconnected4 Enables connection to a frequency counter.5 Enables connection to an external power monitor (5 mv = 1 watt)6 Ground7 Energizestheultrasonicswhen connected to ground.8 and 9 Enables the intensity to be remotely adjusted using an external 10k potentiometer. See belowPREPARATION FOR USE1. Plug the electrical line cord into the electrical outlet, if the unit is already on; depress2. If the optional footswitch is used, insert the plug into the jack located on the rearpanel. Make sure that the plug is inserted forcefully all the way in.3. If the converter / probe assembly is not already assembled; screw the probe into theconverter and secure with wrenches provided.4. Mount the converter / probe assembly in a laboratory stand, secure the clamp to theupper section of the converter housing only. Do not secure the clamp to the probe.5. Connect the converter cable to the power supply.USING THE ULTRASONIC PROCESSORand the following control parameters.AMPLITUDE: The amplitude is the only parameter that must be set in order for the Ultrasonic Processor to be operational. The other control parameters – Timeand Pulse, do not have to be set for continuous operation. AMPL. displays theamplitude selected e.g. 40%. To set the amplitude when the ultrasonics is off,press the AMPL key and the numeric keys for a 40% reading on the screen –Ampl 40%, and the ENTER/REVIEW key.The screen will display:The Ultrasonic Processor is now ready for continuous operation. To energize the ultrasonics, press the START key or the footswitch. To de-energize the ultrasonics, press the key or release the footswitch. If the Time or Pulse functions must be used, refer to the appropriate paragraph(s) below.To increase or decrease the amplitude in small increments when the ultrasonics is on, depress the AMPL key to display Amplitude Setting on the screen, then depress the ▲or ▼key, as required.Immerse the microtip half way into the sample. If the probe is immersed to an insufficient depth, air will be injected into the sample, causing the sample to foam.Since the amplitude required is application dependent and subject to the volume and composition of the sample, it is recommended that the amplitude be selected empirically, by increasing or decreasing the level of intensity as required as the sample is being processed.TIMER:In the pulsed mode the processing time will be different from theelapsed time because the processing time function monitors and controls only the ON portion of the duty cycle. For example, for 1 hour processing time, theelapsed time will be 2 hours if the ON and OFF cycle are set for 1 second. To set the processing time, press the TIMER key.The screen will display:Using the numeric keys, set the processing time as required:Press the ENTER/REVIEW key. The screen will display:PULSER:By inhibiting heat build-up in the sample, the pulse function enablessafe treatment of temperature sensitive samples at high intensity. In addition,pulsing enhances processing by allowing the material to settle back under theprobe after each burst. The ON and OFF pulse duration can be set independently from 01 second to 59 seconds. During the OFF portion of the cycle, the redindicator on the key will illuminate. If the OFF portion of the cycleexceeds three seconds, a cautionary message - Sonics in OFF Cycle - will warnthe operator against touching the ultrasonic probe. To set the pulser, press PULSE key.The screen will display:Using the numeric keys, set the ON portion of the cycle, and press the ENTER/REVIEW key.The screen will display:Using the numeric keys set the OFF portion of the cycle.The screen will display:Press the ENTER/REVIEW key.The screen will display:REVIEW: The REVIEW function provides a “window” on the process by displaying various operating parameters without process interruption. Pressing theENTER/REVIEW key repeatedly during processing will consecutively display the following information.a) Selected amplitude:e.g. Amplitude 40%b) Selected processing time and elapsed processing time:e.g. Set 0:30:00 Time 0:22:10c) Selected pulsing cycle, and actual pulsing cycle:e.g. Pulse 01 01 / 01 00d) Amount of power in watts, and accumulated amount of energy in JOULESdelivered to the probe:e.g. 20 watts 0000000 Joulese) Elapsed time since processing was initiated:e.g. Elapsed time 0:44:20SECTION III – SERVICE INFORMATIONYour Ultrasonic Processor was designed to provide you with years of safe anddependable service. Nevertheless, because of component failure or improper usage, the possibility does exist that it might not perform as it should, shut down due to an overload condition or that it will stop working all together. The most probable causes formalfunction are listed below and should be investigated.The unit was plugged into an electrical outlet that provides a different voltage fromthat required. See Electrical Requirements.The probe and/or microtip is not secured properly.If the probe has a replaceable tip, the tip is not secured properly, or the probe hasbeen used with samples containing solvents or low surface tension liquids.1. the electrical outlet.2. holder from its housing.3.Replace the fuse(s).4.disconnect the probe from the converter.5. reads above 10 watts, either the converter or power supply has failed and thecomplete Ultrasonic Processor should be returned for repair.OVERLOAD CONDITIONIf the Ultrasonic Processor stops working, and an OVERLOAD indication is displayed onRETURN OF EQUIPMENTIt is suggested that an Ultrasonic Processor in need of repair be sent back to the factory.In order to receive prompt service; always contact the factory before returning anyinstrument. Include date of purchase, model number and serial number. For instruments not covered by the 3-year warranty, a purchase order should be forwarded to avoidunnecessary delay. Care should be exercised to provide adequate packing to insureagainst possible damage in shipment. The Ultrasonic Processor should be sent to the “Service Department” with all transportation charges prepaid and return of shipment indicated.Please obtain a Return Authorization Number prior to returning the instrument.SECTION IV - OPERATING SUGGESTIONS AND TECHNIQUES DISRUPTING CELLSThe disruption of cells is an important stage in the isolation and preparation of intracellular products. From research levels through to production, many areas of biotechnology, particularly recombinant technology, necessitate the use of ultrasonics for cell disruption. Although some biological products are secreted from the cell or released during autolysis, many others require sonication to release intracellular material. Cell disruption focuses on obtaining the desired product from within the cell, and it is the cell wall that must be disrupted to allow cell contents extraction.Single-cell organisms (micro-organisms) consist of a semipermeable, tough, rigid outer cell wall surrounding the protoplasmic membrane and cytoplasm. The cytoplasm is made up of nucleic acid, protein, carbohydrates, lipids, enzymes, inorganic ions, vitamins, pigments, inclusion bodies, and about 80% water. In order to isolate and extract any of these substances from inside the cell, it is necessary to break the cell wall and protoplasmic membrane. In some cases the cell may excrete the desired substance without assistance, but in most cases, the cells must be lysed and sonicated in order for these substances to be released. Breaking cell membranes and releasing the contents present significant challenges. The process must be fast and thorough to maximize the protein yield. Because the energy applied must be great enough to break the cell membranes or walls, yet gentle enough to avoid physically or chemically damaging cell contents, the Vibra-Cell with its variable intensity capability is ideally suited for this application.The level of intensity that should be used is application dependent. For example high intensity might be recommended for the break up of cells, but should never be used when the release of intracellular components might be objectionable e.g. Organelle isolation. The ability to control the amplitude at the probe tip is a prerequisite for process optimization. And because each application requires its own set of processing parameters, due to variation in volume and composition, the optimum amplitude can only be determined empirically. When processing a new sample, it is recommended that the amplitude be set first at 50% (30% with a microtip) and then increased of decreased as required.Yeast, gram-positive bacteria, and to a lesser extent, gram-negative bacteria have considerably harder cell walls in comparison to animal cells, and require relatively high power for cell disruption.Gram negative bacteria typically require 10 to 15 minutes of processing, while staphylococcus requires 20 to 30 minutes.Microorganisms differ greatly in their sensitivity to ultrasonic disintegration. For example, the most readily disintegrated are the rod-like forms (bacilli), while the spherical organisms (cocci) are much more resistant. The group Mycobacteria, to which the tuberculosis organism belongs, is particularly difficult to disrupt. Generally, animal cells are more easily disintegrated that plant cells, and red blood cells are more readily disintegrated than muscle cells because they lack a protective cell wall.Cellular disruption is the first step in RNA isolation and one of the most critical steps affecting yield and quality of the isolated RNA. Typically, cell disruption needs to be fast and thorough. Slow disruption, for example placing cells or tissue in guanidinium isothiocyanate (GITC) lysis solution for a long time prior to sonication, may result in RNA degradation by endogenous RNases released internally. This is especially a concern when working with tissues high in endogenous RNase such as spleen and pancreas. Disrupting frozen tissue is more time consuming and cumbersome that processing fresh tissue, but freezing samples is sometimes necessary. Samples are usually frozen when, 1) they are collected over a period of time and thus, cannot be processed simultaneously; 2) there are many samples, 3) samples are collected in the field, or 4) mechanical processing of fresh samples is insufficient for thorough disruption. A mortar and pestle or bag and hammer are typically used when the starting material is frozen. RNA will remain intact in tissues for a day at 37ºC, a week at 25ºC a month at 4ºC and indefinitely at subzero temperatures.Ultrasonic processing will typically cause the temperature of the sample to increase especially with small volumes. Since high temperatures inhibit cavitation, the sample temperature should be kept as low as possible - preferably just above its freezing point. This can be accomplished by immersing the sample vessel in an ice-salt-water bath. Temperature elevation can also be minimized by using the pulser.Increasing hydrostatic pressure (typically 15-60 psi) and viscosity can enhance cell disruption. For microorganisms, the addition of glass beads in the 0.5 to 1mm size range promotes cell disruption. Beads are almost a prerequisite when working with spores and yeast. A good ratio is one volume of beads to two volumes of liquid. Glass beads are available from Cataphote, Inc. P.O. Box 2369, Jackson, Mississippi 39225-2369 USA, phone (800) 221-2574 or (601) 939-4612, FAX (601) 932-5339, Jayco Inc. 675 Rahway Ave., Union NJ 07083 USA, phone (908) 688-3600, FAX (908) 688-6060 or Sigmund Lindner GmbH. P.O. Box 29. D-95483 Warmensteinach, Germany. Phone (49) 0 92 77 9 94 10, FAX (49) 0 92 77 9 94 99.When processing difficult cells such as yeast, pretreatment with an enzyme is beneficial. Lysozyme, byaluronidase, glycosidase, glucalase, lyticase, zymolase and lysostaphin digestion are among the enzymatic methods frequently used with yeast and Lysozyme with bacteria. Enzymatic treatment is usually followed by sonication in a GITC lysis buffer. Collogenase may be used with collogen, lysostaphin with staphylococcus, and trypsin hyaluronidase with liver and kidney.If enzymes cannot be used, the following procedures should be considered: Freezing the sample at -70°C overnight, then thawing it in water immediately prior to ultrasonic processing.Most animal tissues can be processed fresh (unfrozen). It is important to keep fresh tissue cold and to process it quickly (within 30 minutes) after dissection. When working with fresh tissue, the cells must be sonicated immediately at the time the GITC lysis solution is added. This can be done by dispensing the lysing solution in the tube, adding the tissue and immediately sonicating. Samples should never be left sitting in lysis solution, undisrupted. Large samples of hard tissues should be first treated in a blender or a mechanical homogenizer.Animal tissues that have been frozen after collection should be disrupted by grinding in liquid nitrogen with a mortar and pestle. During this process, it is important that the equipment and tissue remain at cryogenic temperatures. The tissue should be dry and powdery after grinding. Grinding should be followed by thorough sonication in a GITC lysis buffer. Processing frozen tissue in this way is cumbersome and time consuming, but effective.Cultured cells are normally easy to disrupt. Cells grown in suspension are collected by centrifugation, rinsed with PBS to remove culture medium, and then lysed by sonicating in a GITC lysis buffer. Placement of the vessel on ice while washing and lysing the cells will further protect the RNA from endogenous RNases released during the disruption process.Soft, fresh plant tissue can often be disrupted by sonicating in a lysis buffer. Other plant tissues, like pine needles, need to be ground dry, without liquid nitrogen. Some hard, woody plant materials require freezing and grinding in liquid nitrogen prior to being ultrasonically processed. Plant cell suspension cultures and calluses can typically be sonicated in a lysis buffer within 2 minutes. The diversity of plants and plant tissue make it impossible to give a single recommendation for all. However, most plant tissues typically contain polysaccharides and polyphenols that can coprecipitate with RNA and inhibit downstream assays. Treating a plant tissue lysate with polyvinylpyrrolidone (PVP) will precipitate such problematic components from the lysate before the actual RNA isolation is carried out.Whenever possible, the tissues should be diced very small to permit movement within the liquid. Tough tissues such as skin and muscle should be macerated first in a blender or the like for about 10 seconds, and confined to a small vessel during ultrasonic treatment. If sub-cellular particles are desired intact, the amplitude should be kept low, and the processing time increased.Yeast can be extremely difficult to disrupt because their cell walls may form capsules or nearly indestructible spores. To process yeast, sonicate in a tube containing the sample, guanidinium-based lysis buffer and small glass beads (0.5 – 1 mm). Pretreatment withzymolase, glucalase and / or lyticase to produce spheroplasts that are readily lysed may also be useful.To disrupt filamentous fungi, scrape the mycelial mat into a cold mortar, add liquid nitrogen and grind to a fine powder with a pestle. The powder can then be thoroughly sonicated in lysis buffer to solubilize completely. As fungi may also be rich in polysaccharides, pretreatment with polyvinylpyrrolidone (PVP) may be beneficial. Bacteria, like plants, are extremely diverse; therefore, it is difficult to make one recommendation for all bacteria. Ultrasonic processing will lyse most Gram positive and Gram negative bacteria, including mycobacteria. Although it is recommended that glass beads and lysis solution be used; it is possible to lyse some Gram negative bacteria by sonicating in lysis solution without beads. Bacteria cell walls can be digested with lysozyme to form spheroplasts. Gram positive bacteria usually require more rigorous digestion and longer processing time. The spheroplasts are then lysed with sonication in GITC lysis buffer.Disruption of cells found in soil and sediments is accomplished one of two ways. One technique isolates the bacterial cells from the material prior to the RNA isolation procedure. This is accomplished by homogenization of wet soil in a mechanical blender followed by a slow speed centrifugation to remove fungal biomass and soil debris. The supernatant is centrifuged again at a higher speed to pellet the bacteria cells. Cells can then be lysed as described above for bacteria. Other techniques describe RNA isolation from the soil or sediment directly. For example, one method requires soil to be added to a diatomaceous earth and lysis buffer, and then sonicated. The sample is then centrifuged to remove solid debris.Always immerse the probe deep enough below the surface of the sample to inhibit aerosoling or foaming, foaming substantially reduces cavitation. Processing at a lower power setting without foam is much more effective than processing at a higher power setting with foam. Decreasing the power, increasing processing time and lowering the temperature of the sample will usually prevent aerosoling and foaming. Do not use any antifoaming agents or surfactants.During cavitation, free radicals are formed which, if they are allowed to accumulate, can greatly affect the biological integrity of the sample by reacting with proteins, polysaccharides, or nucleic acids. Although during short periods of processing their formation is not normally considered a problem; for longer durations, the addition of free radical scavengers such as, carbon dioxide, N2O, cysteine, reduced glutahione, dithiothreitol or other SH compounds, might be beneficial. Saturating the sample with a protective atmosphere of helium or nitrogen gas, or dropping a small pellet of dry ice in the sample, will also inhibit free radical formation. Whereas it is true that gas is required for effective cellular disruption, it is not necessary that the vapor phase be oxygen or air since any gas except carbon dioxide will work just as well.21Various methods can be used to measure the efficiency of the disruption. For example, a visual count can be made using a microscope.For greater accuracy, a protein assay could be used. This procedure is widely recognized as a good method for measuring cell disruption by taking into account the amount of protein released after disruption. The disrupted cells are then tested and checked against this number for percentage breakage.There are several types of protein assays. One commonly used is the Folin Reaction (Lowry Assay) method, as it is comparatively simple and provides consistent results. This colorimetric method has a sensitivity to protein of around 8 µg / mL in the assay solution.The assay turns blue in the presence of proteins due to the reaction of copper ions in the alkaline solution with protein and the reduction of phosphomolybdate- phosphotungstic acid in the Folin reagent by aromatic amino acids in the treated protein.Fractional protein release, Rp, is calculated using the following equation and multiplying the result by 100:Rp = Cf – CbCt – CbCf = Free proteinCt = total proteinCb = Background proteinThis gives the actual disruption percentage, taking into account the background levels of protein before disruption.Since the greatest concentration of energy is beneath the probe, it is imperative that the sample be kept as close to the tip as possible, liquids are easily processed because the free moving cells circulate repeatedly below the probe. Solid materials however have a tendency to be repelled by the ultrasonic, and should be processed in a vessel large enough to accommodate the probe, yet small enough to restrict sample movement. For small samples, conical shaped test tubes are recommended.Allowing the probe to contact the vessel will decrease the power output, and cause minute grey glass particles to migrate into the sample. Although these glass particles will not adversely affect the chemical composition of the sample, they will form a thin grey layer on centrifuging. If the probe has to come in contact with a solid sample, use a standard 20mm (3/4”) diameter stainless steel centrifuge tube cut to 70mm (3”) length. Do not use a glass tube. Microtips must never allowed to come in contact with anything but the liquid, because the stress resulting at the point of contact with a hard surface will cause the microtip to fracture. Although larger probes will not fracture if they come in contact with a glass vessel, they may cause the vessel to fracture.Before each application, place the tip in water or alcohol and energize the power supply for a few seconds to remove any residual substances. If concerned about contamination from previous use, clean the probe with a 20% Virkon solution and rinse with distilled water. Because Virkon®, Dupont's broad spectrum cleaner / disinfectant is effective against a wide range of virus, bacteria and fungi, it is ideally suited for that purpose. For further information regarding Virkon® contact BiosafetyUSA, Inc. 10220 NW 50thStreet, Sunrise Florida 33351, 954-578-6580 *124 or e-mail info@For critical application, probes may be autoclaved.22。

超声波破碎仪使用方法

超声波破碎仪使用方法嘿，朋友们！今天咱来聊聊超声波破碎仪的使用方法。

话说前几天，我在实验室里可真是跟这个小家伙来了一场“亲密接触”。

咱先说说这超声波破碎仪长啥样吧。

它呀，就像一个方方正正的小盒子，上面有各种按钮和显示屏，看着还挺有科技感。

不过一开始，我看着它心里还直犯嘀咕：“这家伙能好使不？”好了，言归正传，说说咋用它。

首先呢，你得把要破碎的东西准备好。

我当时是要破碎一些细胞，就把细胞混悬液小心翼翼地倒进一个专用的小杯子里。

这倒的时候可得注意了，不能洒出来，不然那可就麻烦了。

接着，把这个小杯子放进超声波破碎仪里面。

这里面有个专门的卡槽，得把杯子放正了，不然等会儿工作起来可不得乱晃嘛。

放好杯子后，就开始设置参数了。

这参数设置可不能马虎，得根据你要破碎的东西来调整。

我当时是一边看着说明书，一边小心翼翼地在那按按钮。

哎呀，这感觉就像在玩一个高科技的游戏机，紧张又刺激。

设置好参数后，按下启动按钮，这时候就听见“嗡嗡” 的声音。

嘿，这声音还挺有节奏感。

看着显示屏上的数字在不断变化，我心里那个期待呀。

就好像在等着一个神秘的礼物拆开一样。

在等待的过程中，我可不敢掉以轻心。

一直盯着那个小杯子，生怕出啥问题。

过了一会儿，感觉时间过得可真慢呀。

不过，当听到“叮” 的一声，就知道完成了。

赶紧把小杯子拿出来，看看里面的细胞被破碎得咋样。

哇，效果还真不错！用超声波破碎仪这事儿吧，其实也不难。

只要你按照步骤来，小心操作，就肯定能成功。

下次要是再用到它，我肯定就轻车熟路了。

嘿嘿，这就是我使用超声波破碎仪的经历，希望对大家有帮助哦！。

超声波细胞粉碎仪操作步骤及使用说明

超声波细胞粉碎仪操作步骤及使用说明一、操作步骤：1、打开电源开关，指示灯亮。

2、按“设定”键，显示窗1（工作/间歇）显示“-1”，进入间隔时间设定状态，此时显示窗3 （温度）显示的是原始数据或“--”（以下同），按置数键设定间隔时间（建议1秒）。

3、按功能键，显示窗1显示“-2”，进入超声时间设定状态，按置数键设定超声时间（最好5秒以下，建议1秒）。

4、按功能键，显示窗1显示“-3”，进入全程时间设定状态，按置数键设定全程时间（此时时间单位为分）。

5、按功能键，显示窗1显示“-4”，进入温度保护设定状态，按置数键设定保护温度（0-40℃）。

6、按设定键结束设定并按“复位”键复位，按启动/暂停键开始超声粉碎，全程时间到后显示显示窗闪烁跳动并自动报警停止工作。

7、如需重复上述实验，先按清零再按启动键。

如工作中需要暂停，再次按启动/暂停键。

二、注意事项：1、本机应安放在干净、干燥处，不可放置在潮湿、高温、灰尘及有腐蚀性气体的地方。

2、仪器后面板上变幅杆选择开关应在变幅杆相应位置，新变幅杆应与选择开关对应。

3、容器容量及形状的选择与样品的对少相协调，采用细长形容器破碎效果最好。

4、建议采用短时间多次粉碎，超声时间在5秒以内，间隔时间在5秒以下。

5、变幅杆擦入液面深度在10-15mm 之间，末端离容器底部大于30 mm。

严禁在末端没有插入液体时开机。

6、换能器在支架上要固定牢靠，防止从立杆上滑下。

变幅杆末端切勿碰撞防止变形或损伤。

7、用一段时间后变幅杆末端会被空化腐蚀而发毛，可用锉刀锉平。

多次锉磨后变幅杆变短，则应根据实际情况把选择开关拔到相应位置。

8、破碎量在5ml 以下选用Φ2 变幅杆，此时变幅杆的插入深度在1 mm 左右，末端离底部不得小于5 mm，超声时间最好在0.5-1 秒之间。

9、旋转功率调节按钮调节功率，应把功率开得小些，以免变幅杆过载而断裂。

切不可超过右侧窗口显示的最大功率。

10、当由于外部信号出现故障或出现保护指示情况时，保护指示灯亮，仪器停止工作，提示工作人员排除故障，待故障排除后按保护复位键恢复正常工作。

超声波细胞破碎仪使用说明

超声波细胞破碎仪使用说明
超声波细胞破碎仪是一种常用的实验室设备，可以用于细胞、组织等生物样品的破碎和分散。

以下是超声波细胞破碎仪的使用说明： 1. 准备工作：将所需细胞、组织等样品放入破碎管中，并加入适量缓冲液或生理盐水，注意不要过多，以免影响破碎效果。

2. 超声波参数设置：根据不同的样品和试验需要，设置合适的超声波参数，包括功率、时间、循环间隔等。

在设置时应当参考相关的文献、经验和实验结果，避免超声波过度破坏样品。

3. 破碎操作：将破碎管插入超声波探头，调整超声波位置，按下启动按钮开始破碎。

在破碎过程中应当定期检查破碎样品状态，避免过度破坏或样品污染。

4. 清洗和消毒：破碎结束后，将超声波探头取出并清洗，以避免残留样品污染下一次破碎。

同时还要对破碎管和其他部件进行清洗和消毒，以保证实验的准确性和可靠性。

5. 维护保养：定期检查超声波设备的电源线、探头等部件是否正常，如发现异常应及时维修或更换，以延长设备使用寿命和保证实验效果。

总之，使用超声波细胞破碎仪需要严格遵守相关操作规程和注意事项，注意样品处理和超声波参数设置，以保证实验结果的准确性和稳定性。

超声破碎仪使用方法

超声破碎仪使用方法
超声破碎仪啊，这可是个厉害的家伙！它就像是一个无声的超级英雄，能把那些顽固的物质打得粉碎。

你知道怎么用它吗？嘿，其实很简单啦！先把你要处理的东西放进去，就像给它送上一份特别的礼物。

然后呢，调节好各种参数，这就像是给这个超级英雄设定好战斗模式。

超声破碎仪工作起来可带劲了！那强烈的声波，就如同无数看不见的小拳头，不停地捶打着目标。

你能想象吗？就好像是一场微小世界里的激烈战斗。

它能处理各种样本，不管是液体还是固体，都能被它轻松搞定。

这不就像是一个全能选手，什么场面都能应对自如吗？
在使用的时候，可一定要注意安全哦！别不小心碰到那些正在激烈战斗的区域，不然可能会被误伤呢。

而且要仔细设置参数，可不能马虎，不然怎么能达到最好的效果呢？
它的应用范围那可广了去了，在生物领域、化学领域都有着重要的地位。

就好像是一个无处不在的秘密武器，为科学研究和实验立下汗马功劳。

超声破碎仪真的是个神奇的工具，它能帮助我们突破很多难题，让我们的实验和研究更加顺利。

不用它，那可真是太可惜啦！所以啊，大家都要好好了解它，好好利用它，让它发挥出最大的威力！。

超声破碎仪使用说明

使用说明：1.旋转功率调节旋钮以调节输出功率大小；间隙时间和超声时间由超声时间设定旋钮来调节，总工作时间由总时间设定置数码开关来设置。

设置好后，按工作复位键进入超声工作；2.按样品量的多少选择适当的容器（试管或各种烧杯及离心管），固定或安放好后，调节振动系统位置，使变幅杆末端插入样品液面10-15mm并使其在容器的中心位置，不得让变幅杆与容器相接触。

变幅杆末端离容器一般应大于30mm。

量少时，功率开得较小的情况下可小于30mm；3.将功率调节旋钮向逆时针方向转至最小位置，工作时间、超声时间和间隙时间调至所需的合适时间（一般工作时间不宜开的过长，且间隙时间应大于工作时间）。

上述准备就绪即可按电源开关开机，再按一次保护复位按钮及工作复位按钮，待设定的间隙时间过后即可进入振荡状态，显示屏开始显示工作时间，将功率调节旋钮慢慢向顺时针方向转动，调至所需的功率位置上，以达到理想的工作效果，待设定的工作时间过后，时间显示窗所设定的总时间。

仪器处于停振状态，如需重复上述实验，可按工作复位键，如不需要重复，应关机并切断电源。

4.如在工作是保护指示灯亮（在保护复位键上），说明仪器的功率开得过大而进入保护状态。

减少功率，按一次保护复位键及工作复位键，即开始工作；5.调换探头时，按探头的规格，相应调节变幅杆选择开关（机箱背面）；注意事项1.严禁在变幅杆未插入液体内（空载）时开机，否则会损坏换能器或超声波发生器；2.换能器在支架上要固定牢靠，防止从立柱上突然下滑，变幅杆末端切勿碰撞，防止变形或损伤；3.对各种细胞量的多少、时间长短和功率大小，有待用户根据各种不同介质摸索确定，选取最佳值；4.用一定时间后变幅杆末端会被空化腐蚀而发毛，可用油石或细什景锉刀锉平，否则会影响工作效果；5.功率表显示的数值与电压、变幅杆插入页面深度及负载（被破碎样品的浓度、稠度）有关，电源电压低于220V，变幅杆插入液面深，负载浓度太浓，显示数值可能要稍小；反之稍高。

超声波细胞破碎仪操作规程

1、目的规范实验室超声波细胞破碎仪的操作维护方法，保证实验检测的有效性。

2、适用范围：适用于实验室超声波细胞破碎仪的使用和维护。

3、职责分子生物学实验室PCR检测岗位人员必须熟练掌握超声波细胞破碎仪的使用方法，并定期做好维护保养和状态检查工作。

4、作业程序4.1操作规程4.1.1确认ON/OFF电源开关置于ON。

4.1.2把电源线插进电源插座。

4.1.3如果使用选配的脚踏开关，把插头插入后面板上的插座中。

确认插头插牢和插到位。

4.1.4检查变换器和探头或者直微端头配合表面的清洁度，以及螺杆螺孔的清洁度。

检查螺杆是否拧紧。

4.1.5用手把探头或者直微端头组件（由连接器和直端头组成）组装到变换器上，用随机附送的搬手拧牢靠4.1.6向探头上固定可更换端头、延长器或者锥形的微端头使用活搬子或者花搬子.4.1.7把变换器电缆连接到电源单元上。

4.1.8把变换器、探头组件安装在实验室支架上。

只把夹具固定到1/2英寸（63毫米）直径的变换器壳上。

不要把夹具固定到变换器/探头组件的其它部分上4.2 维护保养规程4.2.1如果探头必须与固体样品接触，请使用切成70毫米长的20毫米直径不锈钢管。

不要用玻璃管.4.2.2微端头只能与液接触，不得与其它物体接触4.2.3在每次使用前，把探头端头放在水或者酒精中并且通电源数秒以去除残余物4.2.4探头可以用高压蒸锅消毒，也可以浸入开水中消毒，也可以用消毒灭菌剂消毒.4.2.5不得把锥形微端头用在连接器上。

没有连接器不要用直端头。

在处理低表面张力液体时不得使用带螺纹端和可更换的端头的探头.。

超声波细胞粉碎机使用

超声波细胞粉碎机使用
⒈使用前，用清水清洗超声波探头，并用纸将探头擦干净。

⒉将烧杯装的待破碎溶液与盒装冰水混合物放入探头下，抬升底座，使探头深入溶液内，与烧杯的底部有1CM的间距，并将探头放置烧杯中央。

随手关上门。

⒊打开仪器开关，连续按两次设置键，设置总时间，超声时间，超声间隔时间，并按下确定键。

⒋点击“开”，通过玻璃观察超声情况，如有异常，立即点“停”。

重新摆放，确认无误后，开始超声。

⒌超声结束后，拿出破碎溶液，用20%乙醇清洗超声波探头。

注意事项：⑴严禁在超声波探头未插入溶液中时开机，否则会损坏仪器。

⑵根据细胞破碎的量的多少，选择时间长短。

⑶盒装冰水混合物中的水不能超过烧杯中待破摔液的高度，否则在超声破碎时，烧杯会不停的旋转，使探头不在超声的最佳位置。

⑷烧杯底部与探头的间距不能太接近，否则超声时，烧杯底部会破裂。

超声波细胞粉碎机使用说明书

超声波细胞粉碎机使用说明书一、引言超声波细胞粉碎机是一种高效、安全的实验室设备，广泛应用于生物医学研究领域。

本文将详细介绍超声波细胞粉碎机的使用方法和注意事项。

二、设备介绍超声波细胞粉碎机是一种利用超声波振荡原理将生物样本细胞破碎的实验设备。

它由以下几个主要部分组成： 1. 超声波发生器：产生高频超声波信号； 2. 超声波探头：将超声波信号传递给样本； 3. 操作面板：用于设置超声波的参数和控制设备。

三、使用方法1. 准备工作在使用超声波细胞粉碎机之前，需要进行以下准备工作： - 确保设备已连接电源并处于正常工作状态； - 清洁超声波探头，确保其表面干净； - 准备待处理的生物样本。

2. 设置参数在操作面板上设置超声波的参数，包括： - 超声波功率：根据样本的特性和所需破碎程度进行调整； - 超声波时间：设置超声波处理的时间； - 工作模式：选择连续工作模式或脉冲工作模式。

3. 进行样本处理将待处理的生物样本放置在超声波探头下方的样本槽中，确保样本与超声波探头接触良好。

然后按下启动按钮，超声波细胞粉碎机开始工作。

4. 注意事项•在操作过程中，避免将手指或其他物体接触到超声波探头；•根据样本的特性和所需破碎程度，适时调整超声波的参数；•定期清洁超声波探头，避免污染样本；•在处理高浓度样本时，可以适当降低超声波功率，以防止样本过热。

四、常见问题解答1. 超声波细胞粉碎机可以处理哪些样本？超声波细胞粉碎机可以处理多种生物样本，包括细胞、组织等。

2. 如何选择超声波的功率和时间？选择超声波的功率和时间需要根据样本的特性和所需破碎程度来决定。

一般来说，样本越难破碎，需要使用更高的功率和更长的时间。

3. 超声波细胞粉碎机是否需要定期维护？是的，超声波细胞粉碎机需要定期清洁超声波探头，避免污染样本。

同时，也需要定期检查设备的工作状态，确保其正常运行。

4. 超声波细胞粉碎机是否适用于敏感样本？超声波细胞粉碎机在处理敏感样本时需要谨慎操作，可以降低超声波的功率，以避免样本受损或过热。

超声波破碎仪使用操作步骤

超声波破碎仪使用操作步骤1.插上电源线。

2.打开仪器后下方的电源开关，指示灯亮。

3.通过“▲▼”键来设定“超声时间、间隙时间、工作次数”。

一般超声时间不宜开的过长，控制在1-5秒为宜，工作时最好选用间隙时间大于工作时间进行实验。

4．通过仪器底下的旋钮“●”来调整功率，功率不要超过200W。

5．根据样品量选用适配容器（试管、烧杯及离心管），固定好后，调节隔音箱内的升降台来确定高低位置，使变幅杆末端插入样品液面一定深度，并使其在容器的中心位置，不得让变幅杆与容器壁及容器底相接触。

6.关上隔音箱门，按“启动/暂停”键启动超声破碎，超声结束后按“复位“键。

超声过程中可按“启动/暂停”键停止超声，若要恢复超声则再按“启动/暂停”键。

7.样品超声结束后，关掉仪器后下方的电源开关。

注：超声前后请用水冲洗变幅杆，并擦干甩片机使用操作步骤1.插上电源线。

2.打开仪器前方的电源开关，指示灯亮。

3.程序设置：重复按“设置”键，显示屏上的“转速”“时间”“离心力”“转头”等设置项目依次闪动，这时可通过“▲▼”上下键对闪动的项目进行设置。

设置完所有项目后，按“确定”键保存。

4.向外抽仪器右上侧的拉动杆，打开盖子，把准备好的甩片装置放到架子上。

5. 将细胞悬液加入甩片装置中，盖上盖子，按“启动”键开始运行。

仪器运行过程中可按“停止”键暂停。

6. 运行结束后，打开盖子，取出甩片装置。

盖上盖子，关掉电源开关。

低速台式离心机使用操作步骤1.插上电源线。

2.打开仪器左下方的电源开关，指示灯亮。

3.程序设置：重复按“SET”键，显示屏上的“Roter””Speed/Rcf”等设置项目的数值依次闪动，这时可通过“▲▼”上下键对闪动的项目进行设置。

设置完所有项目后，按“ENTER”键保存。

4．按“STOP”键打开离心机的盖子，将配平好的离心管放入离心机。

用力盖紧盖子后，按”START”键启动运行。

5.离心结束后，按“STOP”键打开离心机的盖子，取出离心管。

超声波使用说明书

N&DN系列(LCD)超声波细胞粉碎机使用说明书宁波新芝生物科技股份有限公司地址：宁波市国家高新技术园区木槿路65号 315013电话：传真：网址：一. 概述随着生物产业的发展，应用超声波细胞粉碎机所做的实验要求也随之提高，如对样品温度的测定、控制，低温冷却样品及整机的智能化程度的提高等等，都提出了新的要求，为进一步完善此类仪器的各项性能，我公司在现有各种型号的超声波细胞粉碎机的基础上，吸收国外最新技术，结合微电脑控制、选频、测温、保护等软硬件技术而研制的超声波细胞粉碎机，它具有技术先进、性能可靠、操作简便、外型美观、显示清晰明亮、测温控温精确等优点。

该机广泛应用于生物化学、微生物学、药物化学、表面化学、物理学、动物学、农学、医学、制药等领域教学、科研、生产。

Ф18 3/4”20-25KHz 200-950W 200 - 500mlФ20 3/4” -1000mlФ25 1” -1200ml本机由超声波发生器和超声波换能器组件两大部分组成。

其电原理由整流电源，开关电源、变频系统、功率放大器、锁相频率自动跟踪器、功率调节器、功率检测器、功率保护器及微电脑控制等组成。

换能器组件是由压电振子、变幅放大器所组成的产生机械能的聚能变幅装置。

仪器功能键说明（见附图）：变幅杆选择开关航空插(超声)保险丝-8A保险丝-5A电源插座五. 参数设置：图2.面板和按键1.按安装图说明安装好本仪器，用专用的电源线连接发生器背面的电源插座，把换能器组件的信号输入接头与信号输出接口连接好。

超声波破碎仪操作流程

超声波破碎仪操作流程超声波破碎仪是一种常用的实验室设备，用于将样品破碎成微小颗粒或分子。

本文将介绍超声波破碎仪的操作流程，以帮助使用者正确使用该设备并获得高质量的样品破碎结果。

一、设备准备1. 确保超声波破碎仪处于稳定的工作台面上，并接通电源。

2. 检查超声波探头是否正确安装在破碎仪的振荡杆上，并确保其与样品接触的部分干净且无污染。

3. 准备好所需的样品，并将其放入适合的破碎仪容器中。

确保容器干净，并在破碎前根据需要添加适量的溶液或缓冲液。

二、设定操作参数1. 打开破碎仪的控制面板，选择所需的工作模式（如连续模式或脉冲模式）和破碎时间。

2. 根据样品的特性和需要，调整超声波功率和振幅。

一般来说，较硬或较难破碎的样品需要较高的功率和振幅。

三、进行破碎操作1. 将装有样品的容器放入超声波破碎仪的固定夹具中，并确保夹具牢固地固定容器，避免破碎过程中的晃动或跌落。

2. 关闭破碎仪的密封盖或安全盖，并确保其紧密密封。

3. 启动破碎仪，开始进行样品破碎。

在破碎过程中，可以通过控制面板监控功率、振幅和破碎时间等参数，并根据需要进行调整。

4. 监视破碎过程中的溶液温度，并确保其在可接受范围内，以避免样品的过度加热或损坏。

5. 根据所需的破碎程度和时间，适时关闭破碎仪并取出破碎后的样品。

注意在取出样品之前关闭超声波发生器以避免伤害。

四、清洁和维护1. 使用清洁剂和干净的布或纸巾清洁超声波探头和容器，将残留的样品和杂质完全清除。

2. 检查超声波探头是否有损坏或磨损，如有需要及时更换。

3. 定期检查和清洁破碎仪的工作台面、控制面板和电缆等部分，保持设备整洁并延长其使用寿命。

总结：超声波破碎仪操作流程包括设备准备、设定操作参数、进行破碎操作以及清洁和维护。

正确使用超声波破碎仪可以获得高质量的样品破碎结果，并保证设备的正常运行和寿命。

在操作过程中请务必注意安全，遵循设备的使用说明和操作规范。

bioruptor 非接触式超声破碎

Bioruptor是一种非接触式超声破碎设备，利用超声波在液体中产生的高频振动来破碎细胞和组织。

与传统的破碎方法相比，Bioruptor具有许多优点。

首先，它是一种非接触式破碎方法，可以避免对样品产生污染和交叉污染。

其次，Bioruptor 的破碎效果非常好，可以快速破碎细胞和组织，且破碎程度均匀，能够提高实验的重复性和准确性。

此外，Bioruptor还具有操作简便、易于清洁和维护等特点，能够大大提高实验效率。

在实际应用中，Bioruptor可以用于各种需要破碎细胞和组织的实验，如蛋白质组学、基因组学、细胞培养等。

它不仅可以用于实验室研究，还可以应用于生物技术、制药、农业等领域。

此外，Bioruptor还可以与其他实验设备结合使用，如离心机、细胞培养箱等，以实现更全面的实验方案。

总之，Bioruptor作为一种非接触式超声破碎设备，具有破碎效果好、操作简便、易于清洁和维护等特点。

它能够提高实验的重复性和准确性，大大提高实验效率。

在各个领域的研究和实验中，Bioruptor已经成为一种不可或缺的实验工具。

超声细胞粉碎机操作规程

超声细胞粉碎机使用说明打开电源开关(机箱后面板左下角)，此时屏幕显示“新芝商标”。

从启动界面进入换能器选择界面，首先必须选择变幅杆的型号，即“变幅杆：ΦX”中的X会不停的跳动，请选择与您安装上去相应的变幅杆型号，通过键盘上的数字键来选择变幅杆的规格。

选择完后按SET或ENTER确定即进入工作界面。

参数设置按设定键(SET)进入参数设置进入后，通过(▲)或(▼)来选择不同的设置项，某项设置数值的末位不停的跳动，表示已进入该项的设置状态。

1.超声开时间设定通过(▲)或(▼)来选择超声开时间设置项(“超声开 0.1s”的首位跳动)，同样只需键入需要的数字即可。

2.超声关时间设定通过(▲)或(▼)来选择超声关时间设置项(“超声关 1.0s”的首位跳动)，同样只需键入需要的数字即可。

3.槽温度设定通过(▲)或(▼)来选择槽温度设置项(设置界面里温度计上方的“30℃”的首位跳动)，同样只需键入需要的数字即可。

4.超声波输出功率设定通过(▲)或(▼)来选择超声波输出功率设置项(设置界面里功率输出条下方的“10%”的首位跳动)，同样只需键入需要的数字即可。

5.设置参数存储、保存在工作界面下，按ENTER，进入预设参数调用，选择相应的模式数(即存储单元)按(SET)实施调用。

调用某一存储单元，对其设置参数进行改动后，系统将自动存入。

超声工作中的设置1.超声工作中的功率调节在超声工作中用户只需要按(▲)或(▼)即可调节超声波的输出功率(按(▲)是增加功率，按(▼)是减少功率，本功能用与功率微调)，在调节时仪器的的输出功率是不停止的，在用户调节到需要的值后即可超声输出。

2.超声停止输出用户只需按停止，即可停止超声输出。

超温保护后的恢复设置在非超声工作状态中，如出现超温保护现象，用户只需要按SET或ENTER键，从新设置槽温度即可。

在超声工作状态中，出现用户超温保护现象必须先按停止，然后在按SET或ENTER键来修改槽温度即可。

超声破碎仪使用说明

使用说明：旋转功率调节旋钮以调节输出功率大小；间隙时间和超声时间由超声时间设定旋钮来调节，总工作时间由总时间设定置数码开关来设置。

设置好后，按工作复位键进入超声工作；按样品量的多少选择适当的容器（试管或各种烧杯及离心管），固定或安放好后，调节振动系统位置，使变幅杆末端插入样品液面10-15mm并使其在容器的中心位置，不得让变幅杆与容器相接触。

变幅杆末端离容器一般应大于30mm。

量少时，功率开得较小的情况下可小于30mm；将功率调节旋钮向逆时针方向转至最小位置，工作时间、超声时间和间隙时间调至所需的合适时间（一般工作时间不宜开的过长，且间隙时间应大于工作时间）。

仪器处于停振状态，如需重复上述实验，可按工作复位键，如不需要重复，应关机并切断电源。

如在工作是保护指示灯亮（在保护复位键上），说明仪器的功率开得过大而进入保护状态。

减少功率，按一次保护复位键及工作复位键，即开始工作；调换探头时，按探头的规格，相应调节变幅杆选择开关（机箱背面）；注意事项严禁在变幅杆未插入液体内（空载）时开机，否则会损坏换能器或超声波发生器；换能器在支架上要固定牢靠，防止从立柱上突然下滑，变幅杆末端切勿碰撞，防止变形或损伤；对各种细胞量的多少、时间长短和功率大小，有待用户根据各种不同介质摸索确定，选取最佳值；用一定时间后变幅杆末端会被空化腐蚀而发毛，可用油石或细什景锉刀锉平，否则会影响工作效果；功率表显示的数值与电压、变幅杆插入页面深度及负载（被破碎样品的浓度、稠度）有关，电源电压低于220V，变幅杆插入液面深，负载浓度太浓，显示数值可能要稍小；反之稍高。

此数据为模拟参数，它的大小不影响超声波发射的实际功率；本机不需预热，使用应有良好的接地；在超声破碎时，由于超声波在液体中起空化效应，使液体温度会很快升高，用户对各种细胞的温度要多加注意，建议采用短时间（每次不超过5秒）的多次破碎，同时可外加冰浴冷却；本机采用无工频变压器的开关电源，在打开发生器机壳切勿乱摸，以防触电；本机安放在干燥处，不可放置于潮湿、高温、灰尘较多及有腐蚀性气体等地方；。

细胞破碎使用说明

超声波细胞粉碎机
超声波细胞粉碎机产品说明:
超声波细胞粉碎机是一种利用超声波在液体中产生空化效应的多功能、多用途仪器。

它能用于多种动植物、病毒、细胞、细菌及组织的破碎，同时可用来乳化、分立、匀化、提取、消泡、清晰、纳米材料的制备、分散及加速化学反应等。

仪器被广泛应用于生物学、微生物学、物理学、动物学、农学、制药、化工、污水处理、纳米材料等领域。

超声波细胞粉碎机主要特征:
●超声探头为进口钛合金材质,经久耐用。

●高能效换能器,确保功效强劲。

●振幅自动调节，在不同的负载状况时振幅保持一致。

●工作时间,超声间歇均可设置。

●微机控制,超声功率连续可调。

●集成温度控制防止样品过热。

●隔音箱均采用特殊隔音材料,隔音效果好。

★均可选配低温恒温槽，控温范围：-20~100℃★如选配低温恒温槽,均赠送特制双层破碎杯一只。