英文摘要论文翻译——革兰氏染色原理

Disscusion about Gram stain principle

This paper discusses the Gram stain principle in terms of the specific molecular structure and the different ionization state in different PH solutions of the triphenylmethane dye, which will ionize an ion with a positive electric when in the solution with PH higher than 3.2; the substance composition variance of the bacteria cell walls, the distinction of the molecular composition and the structure of peptidoglycan which is the specific composition of bacteria cell walls, the distinction of acid dissociation and iso-electric point of different bacteria; and the interaction between the stain and bacteria led by the above. This examination will perfect the principle and render it more guiding significance in the corresponding operations. Keywords

Gram stain; crystal violet; peptidoglycan; alcohol; cell wall; lip polysaccharide;

革兰氏染色原理

从染色剂三苯甲烷特有的分子结构、三苯甲烷染色剂在不同pH溶液中有不同的电离状态,当pH>3．2溶液时它将电离为带一个正电荷的离子的特质;以及不同细菌细胞壁的物质组成差异,构成细胞壁特征性物质——肽聚糖分子组成和空间结

构存在的差异,不同细菌酸式解离以及等电点的差异;由此引发染色剂与细菌之间相互作用的角度论述了革兰氏染色的原理。使该理论更加完善,对相应操作和结果分析更具指导意义。

关键词

革兰氏染色; 结晶紫; 肽聚糖; 乙醇; 外膜; 脂多糖;